· Priloga B k PK VF, datum: 26.08.2020 1/79 Attachment B to PK VF, date: 26.08.2020 Seznam metod s podano merilno negotovostjo/mejami metode The list of methods with measurement

Priloga B k PK VF, datum: 26.08.2020 1/79

Attachment B to PK VF, date: 26.08.2020 Seznam metod s podano merilno negotovostjo/mejami metode The list of methods with measurement uncertainty/method limits

* Trenutno veljavni obseg akreditacije je dostopen na spletni strani SA (http://www.slo-akreditacija.si/acreditation/univerza-v-ljubljani-veterinarska-fakulteta/) Z rumeno so označene spremembe od predhodne verzije priloge B.

Inštitut za varno hrano, krmo in okolje; Enota za varno hrano

Št.

No.

Oznaka preskusne metode

Internal/standard reference

number

Preskusna metoda

Test method

Materiali, proizvodi; Merilna negotovost (podana kot razširjena

merilna negotovost U); Meje metode

Material, products; Measurement uncertainty (expressed as expanded

measurement uncertainty U); Method limits

Akreditirano*

Da/Ne

Accredited Yes/No

1. SOP 173, verzija 3

interna metoda/in-house

method

Določanje histamina v ribah in ribjih izdelkih

s HPTLC– presejalna metoda

Determining histamine in fish and fish

products with HPTLC – screening method

ribe in ribji proizvodi (razen proizvodi v slanici) /

fish and fish products (except products in salt water)

(50–200 mg/kg)

LOQ = 50 mg/kg

±28 mg/kg

DA / YES


interna metoda

in-house method

Določanje ostankov skupnih metabolitov

nitrofuranov z LC-MS/MS,

Determining residues of total nitrofurans

metabolites with LC-MS/MS

meso / meat

(0,5–5,0) μg/kg

3-amino-2-oxolidinone (AOZ)

CCα = 0,63 μg/kg

CCβ = 0,76 μg/kg

(1,0 ± 0,37) μg/kg

(1,5 ± 0,65) μg/kg

(2,0 ± 0,43) μg/kg

5-methylmorfolino-3-amino-2-oxazolidinone

(AMOZ)

CCα = 0,57 μg/kg

CCβ = 0,63 μg/kg

(1,0 ± 0,64) μg/kg

(1,5 ± 0,50) μg/kg

(2,0 ± 1,00) μg/kg

1-amino-hydantoine hydrochloride (AHD)

CCα = 0,58 μg/kg

CCβ = 0,66 μg/kg

(1,0 ± 0,38) μg/kg

(1,5 ± 0,77) μg/kg

(2,0 ± 0,59) μg/kg

semicarbazide hydrochloride (SEM)

CCα = 0,59 μg/kg

CCβ = 0,68 μg/kg

(1,0 ± 0,61) μg/kg

(1,5 ± 0,46) μg/kg

(2,0 ± 0,55) μg/kg

3,5-dinitro-salicylic

acid hydrazine (DNSAH)

presejalna metoda / screening method:

CCβ = 0,5 μg/kg

AOZ, AMOZ, AHD, SEM

DNSAH

jajca / eggs

(0,5–5,0) μg/kg

3-amino-2-oxolidinone (AOZ)

CCα = 0,54μg/kg

CCβ = 0,59 μg/kg

(0,5 ± 0,10) μg/kg

(1,0 ± 0,10) μg/kg

(1,5 ± 0,19) μg/kg

5-methylmorfolino-3-amino-2-oxazolidinone

(AMOZ)

CCα = 0,57 μg/kg

CCβ = 0,64 μg/kg

(0,5 ± 0,15) μg/kg

(1,0 ± 0,25) μg/kg

(1,5 ± 0,34) μg/kg

1-amino-hydantoine hydrochloride (AHD)

CCα = 0,57 μg/kg

CCβ = 0,63 μg/kg

(0,5 ± 0,16) μg/kg

(1,0 ± 0,13) μg/kg

(1,5 ± 0,30) μg/kg

semicarbazide hydrochloride (SEM)

CCα = 0,50 μg/kg

CCβ = 0,51 μg/kg

(0,5 ± 0,004) μg/kg

(1,0 ± 0,02) μg/kg

(1,5 ± 0,03) μg/kg

3,5-dinitro-salicylic

acid hydrazine (DNSAH)


CCβ = 0,5 μg/kg

AOZ, AMOZ, AHD, SEM

DNSAH

DA / YES

DA / YES

DA / YES

DA / YES

NE / NO

DA / YES

NE / NO

DA / YES

DA / YES

DA / YES

DA / YES

NE / NO

DA / YES

NE / NO


Določanje domoične kisline v školjkah s

HPLC z UV detektorjem

školjke / shellfish

užitna klapavica (Mytillus galloprovincialis)

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

Determination of Domoic acid in mussles and

finfish by HPLC with UV detection

domoična kislina / domoic acid

(3–75) μg/kg

LOQ = 3 μg/kg


interna metoda

in-house method

Določanje vsebnosti klorida (kot NaCl) v

mesnih izdelkih – Mohrova metoda

Determination of chloride (as NaCl) content

in meat products – Mohr method

mesni izdelki / meat products

(1 – 7) g/100g

± 9 % za vsebnost NaCl ≤ 1,5 g/100 g

± 5 % za vsebnost NaCl > 1,5 g/100 g

DA / YES

5. ISO 1871: 2009 Določanje dušika in beljakovin po Kjeldahlu

Preračun za % beljakovin: faktor 6,25

Determination of nitrogenand proteins by the

Kjeldahl method

Calculation to % of protein: factor 6,25

SOP 424, verzija 3

meso in mesni izdelki /

meat and meat products

N: (1 – 6,5) g/100g

± 3,4 %

mleko / milk

N: (0,4–1) g/100g

±2,5 %

DA / YES

NE / NO

6. AOAC Official Method

2007.04: 2007

Postopek za določanje maščobe, vode in

beljakovin z NIR spektrometrijo

Procedure for determination of fat, moisture

and protein in meat and meat products

SOP 484, verzija 1

meso in mesni izdelki/


Beljakovine/protein

9 – 30 g/100 g

± 6,7 %

Maščobe/fat:

6 – 40 g/100 g

± 10 %

1 – 5,9 g/100 g

± 28 %

Voda/moisture:

30 – 80 g/100 g

± 4,5 %

DA / YES



method

Ugotavljanje ostankov makrocikličnih

laktonov s HPLC-fluorescenco

Determining macrocyclic lactone residues by

HPLC-fluorescence

mleko / milk

ivermektin / ivermectin (IVM)

(0,1–60) μg H2B1a/kg

CCα = 0,1 μg H2B1a/kg

CCβ = 0,14 μg H2B1a/kg

±0,27 μg H2B1a/kg

(2 μgH2B1a/kg)

doramektin / doramectin (DOR)

(0,1–60) μg/kg

CCα = 0,1 μg/kg

CCβ = 0,14 μg/kg

±0,35 μg/kg (2 μg/kg)

abamektin / abamectin (ABM)

(0,1–60) μg B1a /kg

CCα = 0,1 μg/kg

CCβ = 0,14 μg B1a /kg

±0,35 μg B1a /kg (2 μg B1a /kg)

eprinomektin / eprinomectin (EPR)

(0,2–60) μg EPR B1a/kg

CCα = 24,8 μg EPR B1a/kg

CCβ = 27,8 μg EPR B1a/kg

±3,7 μg EPR B1a/kg

(20 μg EPR B1a/kg)

LOD = 0,2 μg EPR B1a/kg

moksidektin / moxidectin (MOX)

(0,2–60) μg/kg

CCα = 50,6 μg/kg

CCβ = 55,2 μg/kg

±6,2 μg/kg (40 μg/kg)

LOD = 0,2 μg/kg

emamektin / eprinomectin (EMA)

(0,2–60) μg EMA B1a/kg

CCα = 0,2 μg EMA B1a/kg

CCβ = 0,27 μg EMA B1a/kg

±0,6 μg EMA B1a/kg

(2 μg EMA B1a/kg)

nemadektin / nemadectin (NEM)

(0,2–60) μg/kg

CCα = 0,2 μg/kg

CCβ = 0,25 μg/kg

±0,3 μg/kg (2 μg/kg)

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

maščoba perutnine / poultry fat


(1–100) μg H2B1a/kg

CCα = 1,1 μg/kg

CCβ = 1,2 μg/kg

doramectin / doramectin (DOR)

(1–100) μg/kg

CCα = 1,1 μg/kg

CCβ = 1,3 μg/kg

abamectin / abamectin (ABM)

(1–100) μg B1a /kg

CCα = 1,1 μg/kg

CCβ = 1,4 μg/kg


(1–100) μg EPR B1a/kg

CCα = 1,2 μg/kg

CCβ = 1,3 μg/kg


(1–100) μg/kg

CCα = 1,1 μg/kg

CCβ = 1,2 μg/kg

emamektin / emamectin (EMA)

(1–100) μg EMA B1a/kg

CCα = 1,2 μg/kg

CCβ = 1,5 μg/kg

nemadektin / nemadectin (1–100) μg/kg

CCα = 1,1 μg/kg

CCβ = 1,2 μg/kg

prašičja maščoba / porcine fat


(1–200) μg H2B1a/kg

CCα = 108 μg/kg

CCβ = 118 μg/kg


(1–250) μg/kg

CCα = 183 μg/kg

CCβ = 204 μg/kg


(1–100) μg B1a /kg

CCα = 1,1 μg/kg

CCβ = 1,3 μg/kg



CCα = 1,2 μg/kg

CCβ = 1,4 μg/kg


(1–100) μg/kg

CCα = 1,1 μg/kg

CCβ = 1,2 μg/kg

emamektin /emamectin (EMA)


CCα = 1,2 μg/kg

CCβ = 1,5 μg/kg


(1–100) μg/kg

CCα = 1,2 μg/kg

CCβ = 1,3 μg/kg

goveja maščoba / bovine fat


(1–200) μg H2B1a/kg

CCα = 107 μg/kg

CCβ = 114 μg/kg


DA / YES

DA / YES

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

(1–250) μg/kg

CCα = 166 μg/kg

CCβ = 179 μg/kg


(1–100) μg B1a /kg

CCα = 12 μg/kg

CCβ = 14 μg/kg



CCα = 276 μg/kg

CCβ = 294 μg/kg


(1–800) μg/kg

CCα = 531 μg/kg

CCβ = 572 μg/kg

emamektin / emamectin (EMA)


CCα = 1,2 μg/kg

CCβ = 1,5 μg/kg


(1–100) μg/kg

CCα = 1,2 μg/kg

CCβ = 1,4 μg/kg



method

Določanje ostankov sulfonamidov s HPLC

fluorescenčno detekcijo

Determining sulfonamide residues by HPLC-

fluorescence

meso / meat

(25–250) μg/kg

sulfatiazol / sulfathiazole (STZ),

±16 μg/kg (100 μg/kg)

CCα = 113 μg/kg

CCβ = 126 μg/kg

sulfadiazin / sulfadiazine (SDZ),

±17 μg/kg (100 μg/kg)

CCα = 112 μg/kg

CCβ = 124 μg/kg

sulfamerazin / sulfamerazine (SRZ),

±15 μg/kg (100 μg/kg)

CCα = 112 μg/kg

CCβ = 124 μg/kg

sulfametazin / sulfamethazine (SMZ),

±14 μg/kg (100 μg/kg)

CCα = 111 μg/kg

CCβ = 123 μg/kg

sulfamoksol / sulfamoxole (SMO),

±20 μg/kg (100 μg/kg)

CCα = 116 μg/kg

CCβ = 133 μg/kg

sulfamonometoksin / sulfamonomethoxine (SMM),

±15 μg/kg (100 μg/kg)

CCα = 113 μg/kg

CCβ = 125 μg/kg

sulfametizol / sulfamethizole (SMTZ),

±38 μg/kg (100 μg/kg)

CCα = 112 μg/kg

CCβ = 124 μg/kg

sulfakloropiridazin / sulfachloropyridazine (SCP),

±17 μg/kg (100 μg/kg)

CCα = 114 μg/kg

CCβ = 128 μg/kg

sulfadoksin / sulfadoxine (SDX),

±16 μg/kg (100 μg/kg)

CCα = 113 μg/kg

CCβ = 127 μg/kg

sulfametoksazol / sulfamethoxazole (SMX),

±17 μg/kg (100 μg/kg)

CCα = 114 μg/kg

CCβ = 129 μg/kg

sulfisoksazol / sulfisoxasole (SFX),

±20 μg/kg (100 μg/kg)

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

CCα = 115 μg/kg

CCβ = 131 μg/kg

sulfadimetoksin / sulfadimethoxine (SDM),

±16 μg/kg (100 μg/kg)

CCα = 113 μg/kg

CCβ = 126 μg/kg

sulfakinoksolin / sulfaquinoxaline (SQX),

±22 μg/kg (100 μg/kg)

CCα = 118 μg/kg

CCβ = 137 μg/kg

sulfafenazol / sulfaphenazole (SPZ)

±18 μg/kg (100 μg/kg)

CCα = 114 μg/kg

CCβ = 129 μg/kg

jajca / eggs

presejalna metoda / screening method

CCβ = 20 μg/kg

sulfisoksazol / sulfisoxasole (SFX)

CCβ = 10 μg/kg

sulfamoksol / sulfamoxole (SMO)

sulfakloropiridazin / sulfachloropyridazine (SCP)

CCβ = 5 μg/kg

sulfatiazol / sulfathiazole (STZ)

sulfadiazin / sulfadiazine (SDZ)

sulfamerazin / sulfamerazine (SRZ)

sulfametazin / sulfamethazine (SMZ)

sulfamonometoksin / sulfamonomethoxine (SMM)

sulfadoksin / sulfadoxine (SDX)

sulfametoksazol / sulfamethoxazole (SMX)

sulfadimetoksin / sulfadimethoxine (SDM)

sulfakinoksalin / sulfaquinoxaline (SQX)


sulfapiridin / sulfapyridine (SPY)

sulfanilamid / sulfanilamide (SAD)

sulfagvanidin / sulfaguanidine (SG)

DA / YES



method

Ugotavljanje prisotnosti sulfonamidov

HPTLC - presejalna metoda

Determining of sulfonamides

HPTLC - screening method

meso / meat

sulfagvanidin / sulfaguanidine (SG)

sulfanilamid / sulfanilamide (SAD)



sulfadiazin / sulfadiazine (SDZ)




sulfametizol / sulfamethizole (SMTZ)





sulfakvinoksalin // sulfaquinoxaline (SQX)

sulfadoksin / sulfadoxine (SDX)


sulfamoksol / sulfamoxole (SMO)

sulfametoksipiridazin / sulfachloropyridazine (SMP)

CCβ = 25 μg/kg

DA / YES


interna metoda

in-house method

Določanje PSP toksinov v školjkah s HPLC-

FLD – presejalna metoda

Determination of PSP toxins in shellfish with

HPLC-FLD – screening method

školjke / mussels

užitna klapavica (Mytilus galloprovincialis)

CCβ = 300 μg/kg

neosaxitoxin (NEO)

gonyautoxin 1 in / and gonyautoxin 4 (GTX1,4)

CCβ = 100 μg/kg

saxitoxin (STX) decarbamoylsaxitoxin (dc STX)

gonyautoxin 5 (GTX 5)

gonyautoxin 2 in / and gonyautoxin 3 (GTX2,3)

decarbamoylgonyautoxin 2 in / and

decarbamoylgonyautoxin 3 (dc GTX2,3)

N-sulfocarbamoyl-gonyautoxin 1 in / and N-

sulfocarbamoyl-gonyautoxin 2 (C1,2)

DA / YES

11. SOP 439, verzija 3 Določanje lipofilnih toksinov v školjkah s školjke / mussels





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

interna metoda

in-house method

LC-MS/MS - presejalna metoda

Determination of lipophilic toxins in shellfish

with LC-MS/MS – screening method

Potrditvena metoda / Confirmatory method

užitna klapavica (Mytillus galloprovincialis)

Presejalna metoda/ screening method

CCβ = 32 μg/kg

okadaic acid group (OA, DTX1, DTX2, DTX3)

pectenotoxins group (PTX1, PTX2)

azaspiracids group (AZA1, AZA2, AZA3)

SPX1

GYM

CCβ = 200 μg/kg

yessotoxins group (YTX, 45 OH YTX, homo YTX,

45 OH homo YTX)

Potrditvena metoda /Confirmatory method

okadaic acid group(OA)

(32–320) μg/kg

CCα = 173 μg/kg

CCβ = 186 μg/kg

(80 ± 8.6 μg/kg)

(160 ± 17 μg/kg)

(240 ± 25 μg/kg)

pectenotoxins group (PTX)

(32–320) μg/kg

CCα = 187 μg/kg

CCβ = 214 μg/kg

(80 ± 15 μg/kg)

(160 ± 36 μg/kg)

(240 ± 42 μg/kg)

azaspiracids group (AZA)

(32–320) μg/kg

CCα = 182 μg/kg

CCβ = 203 μg/kg

(80 ± 11 μg/kg)

(160 ± 26 μg/kg)

(240 ± 50 μg/kg)

SPX1

(32–320) μg/kg

CCα = 179 μg/kg

CCβ = 199 μg/kg

(80 ± 17 μg/kg)

(160 ± 41 μg/kg)

(240 ± 43 μg/kg)

GYM

(32–320) μg/kg

CCα = 182 μg/kg

CCβ = 204 μg/kg

(80 ± 7.9 μg/kg)

(160 ± 27 μg/kg)

(240 ± 32 μg/kg)

yessotoxins group (YTX)

(200–2000) μg/kg

CCα = 4450 μg/kg

CCβ = 5150 μg/kg

(500 ± 99 μg/kg)

(1000 ± 186 μg/kg)

(2000 ± 341 μg/kg)

DA / YES

DA / YES

DA / YES

NE / NO NE / NO

NE / NO NE / NO

NE / NO



method

Določanje ostankov nitroimidazolov in

pripadajočih metabolitov s HPLC-DAD

Determining nitroimidazoles and methabolites

with HPLC-DAD

plazma / plasma: (2 – 12) ng/mL dimetridazol / dimetridazole (DMZ): CCα = 2 ng/ml CCβ = 2,5 ng/ml ±1,2 ng/ml (5 ng/ml) ronidazol / ronidazole (RNZ): CCα = 2 ng/ml CCβ = 2,4 ng/ml ±1,3 ng/ml (5 ng/ml) hidroksidimetridazol / hydroxymetridazole (DMZ-OH): CCα = 2 ng/ml CCβ = 2,3 ng/ml ±1,6 ng/ml (5 ng/ml) metronidazol / metronidazole (MNZ): CCα = 2 ng/ml CCβ = 2,3 ng/ml ±1,3 ng/ml (5 ng/ml) hidroksimetronidazol / hydroxymetronidazole (MNZ-OH): CCα = 2 ng/ml

DA / YES Da / yes Da / yes Da / yes Da / yes Da / yes





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

CCβ = 2,2 ng/ml ±0,8 ng/ml (5 ng/ml) seknidazol / secnidazole (SNZ) CCα = 2 ng/ml tinidatzol / tinidazole (TNZ) CCα = 2 ng/ml hidroksiipronidazol / hydroxyiprondazole (IPZOH) CCα = 2 ng/ml ornidazol / ornidazole (ONZ) CCα = 2 ng/ml mleko / milk dimetridazol / dimetridazole (DMZ) (0,5–12) µg/kg CCα = 0,5 µg/kg CCβ = 0,6 µg/kg (1–12) µg/kg ronidazol / ronidazole (RNZ) CCα = 1 µg/kg CCβ = 1,3 µg/kg hidroksidimetridazol / hydroxymetridazole (DMZ-OH) CCα = 1 µg/kg CCβ = 1,2 µg/kg metronidazol / metronidazole (MNZ) CCα = 1 µg/kg CCβ = 1,3 µg/kg hidroksimetronidazol / hydroxymetronidazole (MNZ-OH) CCα = 1 µg/kg CCβ = 1,2 µg/kg seknidazol / secnidazole (SNZ) CCα = 1 µg/kg tinidatzol / tinidazole (TNZ) CCα = 1 µg/kg hidroksiipronidazol / hydroxyiprondazole (IPZOH) CCα = 1 µg/kg ornidazol / ornidazole (ONZ) CCα = 1 µg/kg jajca / eggs dimetridazol / dimetridazole (DMZ) (0,5–12) µg/kg CCα = 0,5 µg/kg CCβ = 1,0 µg/kg (1–12) µg/kg ronidazol / ronidazole (RNZ) CCα = 1 µg/kg CCβ = 1,4 µg/kg hidroksidimetridazol / hydroxymetridazole (DMZ-OH) CCα = 1 µg/kg CCβ = 1,1 µg/kg metronidazol / metronidazole (MNZ) CCα = 1 µg/kg CCβ = 1,2 µg/kg hidroksimetronidazol / hydroxymetronidazole (MNZ-OH) CCα = 1 µg/kg CCβ = 1,2 µg/kg seknidazol / secnidazole (SNZ) CCα = 1 µg/kg tinidatzol / tinidazole (TNZ) CCα = 1 µg/kg hidroksiipronidazol / hydroxyiprondazole (IPZOH) CCα = 1 µg/kg ornidazol / ornidazole (ONZ) CCα = 1 µg/kg

Ne / No Ne / No Ne / No Ne / No DA / YES Da / yes Da / yes Da / yes Da / yes Da / yes Ne / No Ne / No Ne / No Ne / No DA / YES Da / yes Da / yes Da / yes Da / yes Da / yes Ne / No Ne / No Ne / No Ne / No



method

Ugotavljanje ostankov kinolonov s HPLC-

fluorescenco

Determining quinolone residues by HPLC-

fluorescence

meso / meat

norfloksacin / norfloxacin (NOR)

(20–60) µg/kg

LOQ = 20 μg/kg

±2,5 μg/kg (20 μg/kg)

±3,2 μg/kg (40 μg/kg)

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

±5,9 μg/kg (60 μg/kg)

ofloksacin / orbifloxacin (OFC)

(30–90) µg/kg

LOQ = 30 μg/kg

±5,8 μg/kg (30 μg/kg)

±10,0 μg/kg (60 μg/kg)

±19,4 μg/kg (90 μg/kg)

ciprofloksacin / ciprofloxacin (CPF)

(20–150) µg/kg

±3,6 μg/kg (50 μg/kg)

±5,9 μg/kg (100 μg/kg)

±13,0 μg/kg (150 μg/kg)

perutnina, svinjina, zajci / poultry, porcine, rabbit:

CCα = 105 µg/kg

CCβ = 110 µg/kg

govedina, ovčetina, koze / bovine, ovine, caprine:

CCα = 104 µg/kg

CCβ = 109 µg/kg

ribe / fish:

CCα = 106 µg/kg

CCβ = 112 µg/kg

enrofloksacin / enrofloxacin (EFC)

(5–150) µg/kg

±7,9 μg/kg (50 μg/kg)

±12,0 μg/kg (100 μg/kg)

±24,6 μg/kg (150 μg/kg)

perutnina, ovčetina, koze / poultry, ovine, caprine:

CCα = 110 µg/kg

CCβ = 121 µg/kg

govedina, zajci / bovine, rabbit:

CCα = 106 µg/kg

CCβ = 111 µg/kg

svinjina / porcine:

CCα = 107 µg/kg

CCβ = 115 µg/kg

ribe / fish:

CCα = 108 µg/kg

CCβ = 116 µg/kg

sarafloksacin / sarafloxacin (SAR)

(10–30) µg/kg

LOQ = 10 μg /kg

±1,1 μg/kg (10 μg/kg)

±1,3 μg/kg (20 μg/kg)

±2,8 μg/kg (30 μg/kg)

difloksacin / difloxacin (DIF)

(5–450) µg/kg

±35,1 μg/kg (150 μg/kg)

±68,4 μg/kg (300 μg/kg)

±124,0 μg/kg (450 μg/kg)

perutnina / poultry:

CCα = 358 µg/kg

CCβ = 416 µg/kg

ovčetina in koze / ovine and caprine:

CCα = 463 µg/kg

CCβ = 526 µg/kg

govedina / bovine:

CCα = 423 µg/kg

CCβ = 446 µg/kg

zajci / rabbit:

CCα = 326 µg/kg

CCβ = 352 µg/kg

svinjina / porcine:

CCα = 436 µg/kg

CCβ = 473 µg/kg

ribe / fish:

CCα = 327 µg/kg

CCβ = 354 µg/kg

oksolinska kislina / oxolinic acid (OXO)

(20–150) µg/kg

±8,8 μg/kg (50 μg/kg)

±14,5 μg/kg (100 μg/kg)

±31,3 μg/kg (150 μg/kg)


CCα = 112 µg/kg

CCβ = 125 µg/kg

svinjina, ovčetina in koze / porcine, ovine and

caprine:





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

CCα = 105 µg/kg

CCβ = 110 µg/kg

govedina in zajci / bovine and rabbit:

CCα = 104 µg/kg

CCβ = 109 µg/kg

ribe / fish:

CCα = 105 µg/kg

CCβ = 110 µg/kg

nalidiksična kislina / nalidixic acid (NAL)

(20–60) µg/kg

LOQ = 20 μg /kg

±5,1 μg/kg (20 μg/kg)

±8,8 μg/kg (40 μg/kg)

±18,6 μg/kg (60 μg/kg)

flumekvin / flumequin (FLU)

(10–600) µg/kg

±53,7 μg/kg (200 μg/kg)

±120,8 μg/kg (400 μg/kg)

±220,3 μg/kg (600 μg/kg)


CCα = 501 µg/kg

CCβ = 616 µg/kg

ovčetina in koze / ovine and caprine:

CCα = 241 µg/kg

CCβ = 281 µg/kg

govedina / bovine:

CCα = 220 µg/kg

CCβ = 240 µg/kg

zajci / rabbit:

CCα = 227 µg/kg

CCβ = 255 µg/kg

svinjina / porcine:

CCα = 229 µg/kg

CCβ = 258 µg/kg

ribe / fish:

CCα = 686 µg/kg

CCβ = 772 µg/kg

marbofloksacin / marbofloxacin (MAR)

(20–60) µg/kg

±2,1 μg/kg (20 μg/kg)

±3,1 μg/kg (40 μg/kg)

±5,8 μg/kg (60 μg/kg)

govedina / bovine:

CCα = 158 µg/kg

CCβ = 165 µg/kg

Svinjina:

CCα = 160 µg/kg

CCβ = 170 µg/kg

danofloksacin / danofloxacin (DAN)

(5–300) µg/kg

±7,8 μg/kg (100 μg/kg)

±12,2 μg/kg (200 μg/kg)

±27,9 μg/kg (300 μg/kg)

perutnina, govedina, ovčetina in koze / poultry,

bovine, ovine and caprine:

CCα = 212 µg/kg

CCβ = 224 µg/kg

svinjina in zajci / porcine and rabbit:

CCα = 107 µg/kg

CCβ = 115 µg/kg

ribe / fish:

CCα = 109 µg/kg

CCβ = 118 µg/kg

orbifloksacin / orbifloxacin (ORB)

10–30 µg/kg

LOQ = 10 μg /kg

±2,6 μg/kg (10 μg/kg)

±4,2 μg/kg (20 μg/kg)

±6,9 μg/kg (30 μg/kg)

jajca / eggs

norfloksacin / norfloxacin (NOR)

(20–1000) µg/kg

LOQ = 20 μg /kg

CCα = 20,7 µg/kg

CCβ = 21,4 µg/kg

±1,0 μg/kg (20 μg/kg)

±1,9 μg/kg (40 μg/kg)

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

±2,5 μg/kg (60 μg/kg)

ofloksacin / ofloxacin (OFC)

(30–1000) µg/kg

LOQ = 30 μg /kg

CCα = 33,3 µg/kg

CCβ = 36,5 µg/kg

±5,9 μg/kg (30 μg/kg)

±5,2 μg/kg (60 μg/kg)

±5,8 μg/kg (90 μg/kg)

ciprofloksacin / ciprofloxacin (CPF)

(10–1000) µg/kg

LOQ = 10 μg /kg

CCα = 10,8 µg/kg

CCβ = 11,5 µg/kg

±1,4 μg/kg (10 μg/kg)

±1,9 μg/kg (20 μg/kg)

±1,4 μg/kg (30 μg/kg)


(5–1000) µg/kg

LOQ = 5 μg /kg

CCα = 6,03 µg/kg

CCβ = 7,05 µg/kg


±1,2 μg/kg (10 μg/kg)

±1,4 μg/kg (15 μg/kg)


(10–1000) µg/kg

LOQ = 10 μg /kg

CCα = 10,2 µg/kg

CCβ = 10,5 µg/kg

±0,31 μg/kg (10 μg/kg)

±0,97 μg/kg (20 μg/kg)

±1,6 μg/kg (30 μg/kg)


(5–1000) µg/kg

LOQ = 5 μg /kg

CCα = 5,35 µg/kg

CCβ = 5,69 µg/kg

±0,28 μg/kg (5 μg/kg)

±0,62 μg/kg (10 μg/kg)

±0,92 μg/kg (15 μg/kg)

oksolinska kislina / oxolinic acid (OXO)

(20–1000) µg/kg

LOQ = 20 μg /kg

CCα = 21,1 µg/kg

CCβ = 22,3 µg/kg

±0,87 μg/kg (20 μg/kg)

±1,7 μg/kg (40 μg/kg)

±3,4 μg/kg (60 μg/kg)

nalidiksična kislina / nalidixic acid (NAL)

(20–1000) µg/kg

LOQ = 20 μg /kg

CCα = 21,3 µg/kg

CCβ = 22,7 µg/kg

±1,4 μg/kg (20 μg/kg)

±2,1 μg/kg (40 μg/kg)

±3,8 μg/kg (60 μg/kg)


(10–1000) µg/kg

LOQ = 10 μg /kg

CCα = 11,7 µg/kg

CCβ = 13,3 µg/kg

±2,5 μg/kg (10 μg/kg)

±1,3 μg/kg (20 μg/kg)

±1,7 μg/kg (30 μg/kg)


(20–1000) µg/kg

LOQ = 20 μg /kg

CCα = 21,7 µg/kg

CCβ = 23,3 µg/kg

±2,8 μg/kg (20 μg/kg)

±4,2 μg/kg (40 μg/kg)

±6,2 μg/kg (60 μg/kg)





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No


(5–300) µg/kg

LOQ = 5 μg /kg

CCα = 5,23 µg/kg

CCβ = 5,47 µg/kg

±0,39 μg/kg (5 μg/kg)

±0,67 μg/kg (10 μg/kg)

±0,95 μg/kg (15 μg/kg)


(10–1000) µg/kg

LOQ = 10 μg /kg

CCα = 10,5 µg/kg

CCβ = 11,0 µg/kg

±0,54 μg/kg (10 μg/kg)

±0,98 μg/kg (20 μg/kg)

±1,4 μg/kg (30 μg/kg)


interna metoda

in-house method

Določanje ostankov NSAID z LC-MS/MS

Determining residues of NSAID with LC-

MS/MS

mleko / milk:

karprofen/carprofen (CPF):

(50–1000) μg/kg

CCα = 64,7 μg/kg

CCβ = 84,1 μg/kg

(50 ± 23,1 μg/kg)

(100 ± 31 μg/kg)

(150 ± 41 μg/kg)

ketoprofen / ketoprofen (KPF):

(2–1000) μg/kg

CCα = 2,49 μg/kg

CCβ = 2,97μg/kg

(1,0 ± 0,50 μg/kg)

(2,0 ± 0,66 μg/kg)

(3,0 ± 0,90 μg/kg)

fluniksin / flunixin (FLU):

(1–1000) μg/kg

CCα = 45,4 μg/kg

CCβ = 51,9 μg/kg

(20 ± 2,3 μg/kg)

(40 ± 3,6 μg/kg)

(60 ± 9,3 μg/kg)

tolfenamska kislina / tolfenamic acid (TFA):

(5–1000) μg/kg

CCα = 55,7 μg/kg

CCβ = 62,6 μg/kg

(25 ± 4,5 μg/kg)

(50 ± 8,1 μg/kg)

(75 ± 13,1 μg/kg)

meloksikam / meloxicam (MLX):

(1–1000) μg/kg

CCα = 16,5 μg/kg

CCβ = 17,9 μg/kg

(7,5 ± 1,38 μg/kg)

(15 ± 1,7 μg/kg)

(23 ± 3,2 μg/kg)

5-hidroksifluniksin / 5-hydroxyflunixin (FLU-OH):

(1–1000) μg/kg

CCα = 47,1 μg/kg

CCβ = 50,7 μg/kg

(20 ± 4,1 μg/kg)

(40 ± 8,2 μg/kg)

(60 ± 12,6 μg/kg)

oksifenilbutazon / oxyphenbutazon (OBZ):

(5–1000) μg/kg

CCα = 5,89 μg/kg

CCβ = 6,89 μg/kg

(5,0 ± 3,74 μg/kg)

(10 ± 3,8 μg/kg)

(15 ± 2,9 μg/kg)

diklofenak / diclofenac (DCL):

(2–1000) μg/kg

CCα = 3,03 μg/kg

CCβ = 3,65 μg/kg

(1,0 ± 1,12 μg/kg)

(2,0 ± 1,08 μg/kg)

(3,0 ± 1,16 μg/kg)

fenilbutazon / phenylbutazone (PBZ):

(5–1000) μg/kg

CCα = 6,52 μg/kg

CCβ = 8,31 μg/kg

(5,0 ± 1,70 μg/kg)

(10 ± 2,4 μg/kg)

(15 ± 2,7 μg/kg)

plazma / plasma

DA / YES

NE / NO





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

karprofen / carprofen CPF):

(50–1000) μg/kg

CCα = 62 μg/kg

CCβ = 79 μg/kg

(50 ± 7,1 μg/kg)

(100 ± 6,9 μg/kg)

(150 ± 6,2 μg/kg)

ketoprofen / ketoprofen

(KPF):

(10–100) μg/kg

CCα = 11 μg/kg

CCβ = 12 μg/kg

(10 ± 1,7 μg/kg)

(20 ± 3,0 μg/kg)

(30 ± 3,6 μg/kg)


(1–20) μg/kg

CCα = 1,1 μg/kg

CCβ = 1,3 μg/kg

(1,0 ± 0,16 μg/kg)

(2,0 ± 0,31 μg/kg)

(3,0 ± 0,38 μg/kg)

tolfenamska kislina /

tolfenamic acid (TFA):

(20–200) μg/kg

CCα = 23 μg/kg

CCβ = 24 μg/kg

(20 ± 1,6 μg/kg)

(40 ± 1,7 μg/kg)

(60 ± 4,2 μg/kg)

meloksikam / meloxicam

(MLX):

(5–100) μg/kg

CCα = 5,6 μg/kg

CCβ = 6,2 μg/kg

(5,0 ± 1,0 μg/kg)

(10 ± 1,6 μg/kg)

(15 ± 2,6 μg/kg)

5-hidroksifluniksin / 5-

hydroxyflunixin (FLU-OH):

(1–20) μg/kg

CCα = 1,2 μg/kg

CCβ = 1,4 μg/kg

(1,0 ± 0,32 μg/kg μg/kg)

(2,0 ± 0,38 μg/kg)

(3,0 ± 0,38 μg/kg)

oksifenilbutazon /

oxyphenbutazon (OBZ):

(20–200) μg/kg e

CCα = 25 μg/kg

CCβ = 29 μg/kg

(20 ± 6,2 μg/kg)

(40 ± 8,6 μg/kg)

(60 ± 7,2 μg/kg)

diklofenak / diclofenac

(DCL):

(10–200) μg/kg

CCα = 12 μg/kg

CCβ = 13 μg/kg

(10 ± 3,1 μg/kg)

(20 ± 3,2 μg/kg)

(30 ± 3,5 μg/kg)

fenilbutazon /

phenylbutazone (PBZ):

(20–200) μg/kg

CCα = 26 μg/kg

CCβ = 33 μg/kg

(20 ± 4,0 μg/kg)

(40 ± 8,0 μg/kg)

(60 ± 5,5 μg/kg)

meso / meat:

karprofen / carprofen CPF):

(250–2500) μg/kg

CCα = 592 μg/kg

CCβ = 684 μg/kg

(250 ±32 μg/kg)

(500 ±127 μg/kg)

(750 ±262 μg/kg)

ketoprofen / ketoprofen (KPF):

(2,5–25) μg/kg

CCα = 2,71 μg/kg

CCβ = 2,91μg/kg

NE / NO





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

(2,5 ± 0,46 μg/kg)

(5,0 ± 0,75 μg/kg)

(7,5 ± 1,91 μg/kg)


(5–50) μg/kg

CCα = 12,8 μg/kg

CCβ = 15,7 μg/kg

(5,0 ± 2,0 μg/kg)

(10 ± 3,8 μg/kg)

(15 ± 4,5 μg/kg)

tolfenamska kislina / tolfenamic acid (TFA):

(25–250) μg/kg

CCα = 68,5 μg/kg

CCβ = 87,0 μg/kg

(25 ± 16 μg/kg)

(50 ± 23 μg/kg)

(75 ± 35 μg/kg)

meloksikam / meloxicam (MLX):

(10–100) μg/kg

CCα = 24,7 μg/kg

CCβ = 29,4 μg/kg

(10 ± 3,2 μg/kg)

(20 ± 6,9 μg/kg)

(30 ± 10,6 μg/kg)

5-hidroksifluniksin / 5-hydroxyflunixin (FLU-OH):

(2,5–25) μg/kg

CCα = 3,53 μg/kg

CCβ = 4,55 μg/kg

(2,5 ± 1,75 μg/kg)

(5,0 ± 1,72 μg/kg)

(7,5 ± 2,15 μg/kg)

oksifenilbutazon / oxyphenbutazon (OBZ):

(2,5–25) μg/kg e

CCα = 3,06 μg/kg

CCβ = 3,61 μg/kg

(2,5 ± 0,86 μg/kg)

(5,0 ± 2,12 μg/kg)

(7,5 ± 2,34 μg/kg)

diklofenak / diclofenac (DCL):

(2,5–25) μg/kg

CCα = 2,98 μg/kg

CCβ = 3,46 μg/kg

(2,5 ± 0,72 μg/kg)

(5,0 ± 1,37 μg/kg)

(7,5 ± 2,63 μg/kg)

fenilbutazon / phenylbutazone (PBZ):

(2,5–25) μg/kg

CCα = 3,00 μg/kg

CCβ = 3,43 μg/kg

(2,5 ± 0,81 μg/kg)

(5,0 ± 0,97 μg/kg)

(7,5 ± 2,15 μg/kg)


interna metoda

in-house method

Določanje ostankov kokcidiostatikov z LC-

MS/MS

Determining residues of coccidiostats with

LC-MS/MS

jajca / eggs

potrditvena metoda / confirmatory method:

amprol / amprolium (AMP):

(5–50) µg/kg

CCα = 11 µg/kg

CCβ = 13 µg/kg


±1,7 μg/kg (10 μg/kg)

±3,1 μg/kg (15 μg/kg)

klopidol / clopidol CL):

(5–50) µg/kg

CCα = 11 µg/kg

CCβ = 13 µg/kg


±2,5 μg/kg (10 μg/kg)

±3,8 μg/kg (15 μg/kg)

dekokvinat / decoquinate (DEQ)::

(10–100) µg/kg

CCα = 23 µg/kg

CCβ = 26 µg/kg

±4,2 μg/kg (10 μg/kg)

±3,4 μg/kg (20 μg/kg)

±8,1 μg/kg (30 μg/kg)

diklazuril / diclazuril (DI):

(1–10) µg/kg

DA / YES

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

CCα = 2,2 µg/kg

CCβ = 2,5 µg/kg

±0,29 μg/kg (1 μg/kg)

±0,32 μg/kg (2 μg/kg)

±0,49 μg/kg (3 μg/kg)

nikarbazin / nicarbazin (DNC):

(50–500) µg/kg

CCα = 107 µg/kg

CCβ = 114 µg/kg

±12,0 μg/kg (50 μg/kg)

±10,0 μg/kg (100 μg/kg)

±12,5 μg/kg (150 μg/kg)

halofuginon / halofuginone (HAL):

(3–30) µg/kg

CCα = 6,8 µg/kg

CCβ = 7,7 µg/kg

±1,49 μg/kg (3 μg/kg)

±1,01 μg/kg (6 μg/kg)

±1,87 μg/kg (9 μg/kg)

lasalocid / lasalocid (LA)

(75–750) µg/kg

CCα = 172 μg/kg

CCβ = 196 μg/kg

±29,0 μg/kg (75 μg/kg)

±27,9 μg/kg (150 μg/kg)

±50,7 μg/kg (225 μg/kg)

maduramicin / maduramicin (MA):

(1–10) µg/kg

CCα = 2,1 µg/kg

CCβ = 2,3 µg/kg

±0,27 μg/kg (1 μg/kg)

±0,28 μg/kg (2 μg/kg)

±0,35 μg/kg (3 μg/kg)

monensin / monensin (MO):

(1,5–15) µg/kg

CCα = 3,2 µg/kg

CCβ = 3,5 µg/kg

±0,30 μg/kg (1,5 μg/kg)

±0,33 μg/kg (3 μg/kg)

±0,51 μg/kg (4,5 μg/kg)

narasin / narasin (NA):

(1–10) µg/kg

CCα = 2,3 µg/kg

CCβ = 2,7 µg/kg

±0,28 μg/kg (1 μg /kg)

±0,40 μg/kg (2 μg /kg)

±0,39 μg/kg (3 μg /kg)

robenidin / robenidin (ROB):

(12,5–125) µg/kg

CCα = 29 µg/kg

CCβ = 33 µg/kg

±3,5 μg/kg (12,5 μg/kg)

±4,6 μg/kg (25 μg/kg)

±10,9 μg/kg (37,5 μg/kg)

salinomicin / salinomicin (SA):

(1,5–15) µg/kg

CCα = 3,6 µg/kg

CCβ = 4,3 µg/kg

±0,72 μg/kg (1,5 μg/kg)

±0,98 μg/kg (3 μg/kg)

±1,13 μg/kg (4,5 μg/kg)

semduramicin / semduramicin (SED):

(1,0–10) µg/kg

CCα = 2,2 µg/kg

CCβ = 2,4 µg/kg

±0,35 μg/kg (1 μg/kg)

±0,48 μg/kg (2 μg/kg)

±0,34 μg/kg (3 μg/kg)



CCβ = 10 µg/kg

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No


CCβ = 10 µg/kg

dekokvinat / decoquinate (DEQ):

CCβ = 10 µg/kg


CCβ = 1,0 µg/kg


CCβ = 50 µg/kg


CCβ = 3,0 µg/kg

lasalocid / lasalocid (LA):

CCβ = 75 μg/kg


CCβ = 1,0 µg/kg


CCβ = 1,5 µg/kg


CCβ = 1,0 µg/kg


CCβ = 25 µg/kg


CCβ = 1,5 µg/kg


CCβ = 1,0 µg/kg

jetra / liver



perutnina, govedo, svinjina, kunci, ostalo / poultry,

bovine, porcine, rabbit, other

(10–100) µg/kg

CCα = 25 µg/kg

CCβ = 29 µg/kg

±3,3 μg/kg (10 μg/kg)

±6,1 μg/kg (20 μg/kg)

±7,8 μg/kg (30 μg/kg)




(10–100) µg/kg

CCα = 26 µg/kg

CCβ = 32 µg/kg

±4,6 μg/kg (10 μg/kg)

±7,6 μg/kg (20 μg/kg)

±8,2 μg/kg (30 μg/kg)




(10–100) µg/kg

CCα = 25 µg/kg

CCβ = 29 µg/kg

±3,9 μg/kg (10 μg/kg)

±5,8 μg/kg (20 μg/kg)

±6,1 μg/kg (30 μg/kg)


purani, govedo, svinjina, ostalo / poultry, bovine,

porcine, other

(20–200) µg/kg

CCα = 48 µg/kg

CCβ = 57 µg/kg

±4,8 μg/kg (20 μg/kg)

±10,1 μg/kg (40 μg/kg)

±16,9 μg/kg (60 μg/kg)

piščanci, kunci / broiler, rabbit

(250–2000) µg/kg

CCα = 551 µg/kg

CCβ = 602 µg/kg

±35,6 μg/kg (250 μg/kg)

±125,5 μg/kg (500 μg/kg)

±136,4 μg/kg (1000 μg/kg)


purani, govedo, svinjina, kunci, ostalo / poultry,


(50–500) µg/kg

CCα = 107 µg/kg

CCβ = 114 µg/kg

±12,5 μg/kg (50 μg/kg)

DA / YES

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

±12,9 μg/kg (100 μg/kg)

±24,3 μg/kg (150 μg/kg)

piščanci / broiler

(250–2000) µg/kg

CCα = 538 µg/kg

CCβ = 575 µg/kg

±75,3 μg/kg (250 μg/kg)

±92,4 μg/kg (500 μg/kg)

±121,5 μg/kg (1000 μg/kg)




(15–150) µg/kg

CCα = 34 µg/kg

CCβ = 38 µg/kg

±4,9 μg/kg (15 μg/kg)

±5,0 μg/kg (30 μg/kg)

±13,4 μg/kg (45 μg/kg)


svinjina, kunci, ostalo / porcine, rabbit, other

(50–400) µg/kg

CCα = 63 μg/kg

CCβ = 76 μg/kg

±8,1 μg/kg (25 μg/kg)

±16,0 μg/kg (50 μg/kg)

±25,5 μg/kg (100 μg/kg)

perutnina, govedo / poultry, bovine

(25–250) µg/kg

CCα = 113 μg/kg

CCβ = 127 μg/kg

±9,4 μg/kg (50 μg/kg)

±20,1 μg/kg (100 μg/kg)

±22,5 μg/kg (150 μg/kg)


purani, govedo, svinjina, kunci, ostalo / turkey,


(1–10) µg/kg

CCα = 2,6 µg/kg

CCβ = 3,2 µg/kg

±0,38 μg/kg (1 μg/kg)

±0,71 μg/kg (2 μg/kg)

±0,67 μg/kg (3 μg/kg)

piščanci / broiler:

(75–600) µg/kg

CCα = 175 μg/kg

CCβ = 200 μg/kg

±12,7 μg/kg (75 μg/kg)

±30,7 μg/kg (150 μg/kg)

±45,4 μg/kg (225 μg/kg)


perutnina, svinjina, kunci, ostalo / poultry, porcine,

rabbit, other:

(4–40) µg/kg

CCα = 11 µg/kg

CCβ = 14 µg/kg



±2,8 μg/kg (12 μg/kg)

govedo / bovine:

(15–90) µg/kg

CCα = 35 μg/kg

CCβ = 41 μg/kg

±2,6 μg/kg (15 μg/kg)

±7,5 μg/kg (30 μg/kg)

±14,7 μg/kg (45 μg/kg)




(25–250) µg/kg

CCα = 66 µg/kg

CCβ = 83 µg/kg

±11,4 μg/kg (25 μg/kg)

±19,8 μg/kg (50 μg/kg)

±33,8 μg/kg (75 μg/kg)


govedo, svinjina, kunci, ostalo / bovine, porcine,





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

rabbit, other:

(25–250) µg/kg

CCα = 29 µg/kg

CCβ = 33 µg/kg

±16,8 μg/kg (25 μg/kg)

±41,0 μg/kg (50 μg/kg)

±44,3 μg/kg (75 μg/kg)


(200–1600) µg/kg

CCα = 491 μg/kg

CCβ = 583 μg/kg

±92,4 μg/kg (200 μg/kg)

±121,3 μg/kg (400 μg/kg)

±243,7 μg/kg (600 μg/kg)



bovine, porcine, rabbit, other:

(2,5–25) µg/kg

CCα = 7,1 µg/kg

CCβ = 9,3 µg/kg

±1,50 μg/kg (2,5 μg/kg)

±3,09 μg/kg (5 μg/kg)

±2,95 μg/kg (7,5 μg/kg)




(1,0–10) µg/kg

CCα = 2,7 µg/kg

CCβ = 3,4 µg/kg

±0,49 μg/kg (1 μg/kg)

±0,87 μg/kg (2 μg/kg)

±0,95 μg/kg (3 μg/kg)





CCβ = 20 µg/kg

clopidol / clopidol (CL):



CCβ = 20 µg/kg


perutnina, govedo, svinjina, kunci, ostalo

/ poultry, bovine, porcine, rabbit, other:

CCβ = 10 µg/kg


purani, govedo, svinjina, ostalo / turkey, bovine,

porcine, other:

CCβ = 20 µg/kg

piščanci, kunci / broiler, rabbit:

CCβ = 500 µg/kg




CCβ = 50 µg/kg


CCβ = 500 µg/kg




CCβ = 15 µg/kg


svinjina, kunci, ostalo / porcine, rabbit, other:

CCβ = 25 μg/kg

perutnina, govedo / poultry, bovine

CCβ = 50 μg/kg




CCβ = 1,0 µg/kg


DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

CCβ = 75 µg/kg


perutnina, svinjina, kunci, ostalo / poultry, porcine,

rabbit, other:

CCβ = 4,0 µg/kg

govedo / bovine:

CCβ = 15 µg/kg




CCβ = 25 µg/kg


govedo, svinjina, kunci, ostalo / bovine, porcine,

rabbit, other:

CCβ = 25 µg/kg


CCβ = 200 μg/kg




CCβ = 2,5 µg/kg

semduramicin / / semduramicin (SED):



CCβ = 1,0 µg/kg


interna metoda

in-house method

Postopek za določanje vsebnosti zaostankov

dapsona v vzorcih mesa, jajc,urina in mleka,

presejalna metoda (HPLC-UV)

Procedure for the determination residues of

dapson in meet, eggs urin and milk, screening

method (HPLC-UV)

jajca / eggs

CCβ = 2,5 µg/kg

meso / meat

CCβ = 2,5 µg/kg

mleko / milk

CCβ = 2,5 µg/kg

DA / YES

DA / YES

NE / NO


interna metoda

in-house method

Ugotavljanje in potrditev ostankov

tetraciklinov z LC-MS/MS

Identification and confirmation of

Tetracyclines residues with Lc-MS/MS

mleko / milk

klortetraciklin / chlortetracycline (CTC):

(5–200) µg/kg

CCα = 112 μg/kg

CCβ = 124 μg/kg

±3,7 μg/kg (50 μg/kg)

±16,0 μg/kg (100 μg/kg)

±21,6 μg/kg (150 μg/kg)

oksitetraciklin / oxytetracycline (OTC):

(5–200) µg/kg

CCα = 110 μg/kg

CCβ = 120 μg/kg

±6,6 μg/kg (50 μg/kg)

±12,7 μg/kg (100 μg/kg)

±34,2 μg/kg (150 μg/kg)

tetraciklin / tetracycline (TC):

(5–200) µg/kg

CCα = 110 μg/kg

CCβ = 120 μg/kg

±5,6 μg/kg (50 μg/kg)

±12,9 μg/kg (100 μg/kg)

±13,2 μg/kg (150 μg/kg)

doksiciklin / doxycycline (DC)::

(5–20) µg/kg

CCα = 5,8 μg/kg

CCβ = 6,7 μg/kg


±1,8 μg/kg (10 μg/kg)

±3,5 μg/kg (15 μg/kg)

krma/feed

CTC, OTC, TC in DC: LOQ = 50 μg/kg

DA / YES

NE / NO



method

Določanje antimikrobnih substanc z UPLC-

MS/MS - mleko

Z UPLC-MS/MS Determination of

antimicrobic substances

with UPLC-MS/MS - milk

mleko / milk


penicilin G / penicillin G:

(2–20) µg/kg

CCα = 4,3 μg/kg

CCβ = 4,6 μg/kg

±0,20 μg/kg (2 μg/kg)

DA / YES

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

±0,31 μg/kg (4 μg/kg)

±0,62 μg/kg (6 μg/kg)

ampicilin / ampicillin:

(2–20) µg/kg

CCα = 4,5 μg/kg

CCβ = 4,9 μg/kg

±0,33 μg/kg (2 μg/kg)

±0,70 μg/kg (4 μg/kg)

±0,82 μg/kg (6 μg/kg)

linkomicin / lincomicin:

(75–750) µg/kg

CCα = 155 μg/kg

CCβ = 160 μg/kg

±6,0 μg/kg (75 μg/kg)

±8,7 μg/kg (150 μg/kg)

±12,0 μg/kg (225 μg/kg)

kloksacilin / cloxacillin:

(15–150) µg/kg

CCα = 34 μg/kg

CCβ = 37 μg/kg

±2,9 μg/kg (15 μg/kg)

±4,6 μg/kg (30 μg/kg)

±6,9 μg/kg (45 μg/kg)

nafcilin / nafcillin:

(15–150) µg/kg

CCα = 33 μg/kg

CCβ = 36 μg/kg

±1,5 μg/kg (15 μg/kg)

±3,1 μg/kg (30 μg/kg)

±4,0 μg/kg (45 μg/kg)

novobiocin / novobiocin:

(25–250) µg/kg

CCα = 56 μg/kg

CCβ = 62 μg/kg

±4,6 μg/kg (25 μg/kg)

±11 μg/kg (50 μg/kg)


cefoperazon / cefoperazone:

(25–250) µg/kg

CCα = 54 μg/kg

CCβ = 58 μg/kg

±2,4 μg/kg (25 μg/kg)

±3,0 μg/kg (50 μg/kg)


ceftiofur / ceftiofur:

(50–500) µg/kg

CCα = 106 μg/kg

CCβ = 113 μg/kg

±3,5 μg/kg (50 μg/kg)

±8,8 μg/kg (100 μg/kg)

±14 μg/kg (150 μg/kg)

eritromicin / eritromicin::

(20–200) µg/kg

CCα = 42 μg/kg

CCβ = 43 μg/kg

±1,4 μg/kg (20 μg/kg)

±2,2 μg/kg (40 μg/kg)

±4,4 μg/kg (60 μg/kg)


amoksicilin / amoksicillin:

CCβ = 2,0 μg/kg

krma/feed

linkomicin / lincomicin:

LOQ = 75 μg/kg

DA / YES

NE / NO



method

Določanje kovin v živilih z ICP – MS

Determination of metals in food with

ICP-MS

mleko / milk:

Pb:

(0,007–1,000) mg/kg

LOD Pb = 0,002 mg/kg

LOQ Pb = 0,007 mg/kg

± 20 % mg/kg (0,007–0,100 mg/kg)

± 15 % mg/kg (0,101–1,000 mg/kg)

DA/YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

Cd:

(0,002-0,500) mg/kg

LOD Cd = 0,0007 mg/kg

LOQ Cd = 0,002 mg/kg

± 20 % (0,002–0,020 mg/kg)

± 10 % (0,021–0,500 mg/kg)

meso / meat

Pb:

(0,01–5,000) mg/kg



± 15 % mg/kg (0,010–0,100 mg/kg)

± 10 % mg/kg (0,101–5,000 mg/kg)

Cd:

(0,005–2,500) mg/kg



± 25 % (0,005–0,020 mg/kg)

± 10 % (0,021–2,500 mg/kg)

jetra in ledvica / liver and kidney

Pb:

(0,06–4,00) mg/kg


LOQ Pb = 0,06 mg/kg

± 20 % mg/kg (0,06–0,100 mg/kg)

± 10 % mg/kg (0,101–4,00 mg/kg)

Cd:

(0,01–2,00) mg/kg


LOQ Cd = 0,01 mg/kg

± 20 % (0,01–0,050 mg/kg)

± 10 % (0,051–2,00 mg/kg

jajca in med/egg and honey

Pb:

(0,01–5,000) mg/kg


Cd:

(0,005–2,500) mg/kg


morski sadeži/seafood

Pb:

(0,06–4,00) mg/kg

LOQ Pb = 0,06 mg/kg

Cd:

(0,01–2,00) mg/kg

LOQ Cd = 0,01 mg/kg

DA/YES

DA/YES

NE/NO

NE/NO

20. SOP 462, verzija 4 Ugotavljanje ostankov pomirjeval

Determination of sedatives screening method

jajca / eggs

Klorpromazin / Chlorpromazin:

CC = 2,5 μg/kg

Karazolol / Carazolol:

CC = 0,5 μg/kg

urin / urine


CC = 2,5 μg/kg


CC = 0,5 μg/kg

Azaperon / Azaperon:

CC = 2,5 μg/kg

Azaperol / Azaperol:

CC = 2,5 μg/kg

ledvice / kidney


CC = 2,5 μg/kg

DA / YES

DA / YES

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No


CC = 0,5 μg/kg

Azaperon / Azaperon:

CC = 2,5 μg/kg

Azaperol / Azaperol:

CC = 2,5 μg/kg

Acepromazine / Acepromazine:

CC = 25 μg/kg

Propiopromazin / Propiopromazine:

CC = 25 μg/kg

mleko / milk:


CC = 2,5 μg/kg


CC = 0,5 μg/kg

DA / YES


interna metoda /

in-house method

Ugotavljanje ostankov kloramfenikola,

florfenikola in tiamfenikola z LC-MS/MS

Determination of chloramphenicol,

florfenicole and tiamphenicol residues with

LC-MS/MS


jajca / eggs

kloramfenikol / chloramphenicol (CAP)

(0,1–10) μg/kg

CCα = 0,14 μg/kg

CCβ = 0,18 μg/kg

±0,05 μg/kg (0,1 μg/kg)

±0,10 μg/kg (0,3 μg/kg)

±0,12 μg/kg (0,4 μg/kg)

florfenikol / florfenicol (FF)

(0,5–50) μg/kg

CCα = 1,83 μg/kg

CCβ = 2,16 μg/kg

±0,15 μg/kg (0,5 μg/kg)

±0,35 μg/kg (1,5 μg/kg)

±0,69 μg/kg (5,0 μg/kg)

tiamfenikol / tiamphenicol (TP)

(1–100) μg/kg

CCα = 3,62 μg/kg

CCβ = 4,24 μg/kg

±0,45 μg/kg (1 μg/kg)

±0,69 μg/kg (3 μg/kg)

±2,05 μg/kg (10 μg/kg)

mleko / milk


(0.1–10) μg/kg

CCα = 0.15 μg/kg

CCβ = 0.20 μg/kg

±0.06 μg/kg (0.1 μg/kg)

±0.09 μg/kg (0.3 μg/kg)

±0.11 μg/kg (0.4 μg/kg)


(0.5–50) μg/kg

CCα = 2.17 μg/kg

CCβ = 2.48 μg/kg

±0.25 μg/kg (0.5 μg/kg)

±0.67 μg/kg (1,5 μg/kg)

±0.96 μg/kg (5.0 μg/kg)


(1–100) μg/kg

CCα = 59 μg/kg

CCβ = 69 μg/kg

±5.4 μg/kg (25 μg/kg)

±10.3 μg/kg (50 μg/kg)

±14.4 μg/kg (75 μg/kg)


jajca / eggs


CCβ = 0,1 μg/kg


CCβ = 0,5 μg/kg

florfenikol amin / florfenicol amine (FFA)

CCβ = 0,5 μg/kg

DA / YES

DA / YES

DA / YES

DA / YES

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No


CCβ = 1,0 μg/kg

mleko / milk


CCβ = 0.1 μg/kg


CCβ = 0.5 μg/kg

florfenikol amin / florfenicol amine (FFA)

CCβ = 0.5 μg/kg


CCβ = 25 μg/kg

DA / YES



method

Določanje sulfonamidov in dapsona v mleku

(s HPLC - DAD)

Determining sulfonamides and dapsone in

milk

(by HPLC - DAD)

mleko / milk


(25–150) μg/L


±11 μg/l (100 μg/kg)

CCα = 109 μg/kg

CCβ = 117 μg/kg


±9 μg/l (100 μg/kg)

CCα = 107 μg/kg

CCβ = 114 μg/kg

sulfadimetoksin / sulfadimetoxine (SDM)

±8 μg/l (100 μg/kg)

CCα = 107 μg/kg

CCβ = 113 μg/kg

sulfadiazin / sulfadiazne (SDZ)

±9 μg/l (100 μg/kg)

CCα = 107 μg/kg

CCβ = 115 μg/kg

sulfamonometoksin / sulfamonometoxine (SMM)

±9 μg/l (100 μg/kg)

CCα = 108 μg/kg

CCβ = 115 μg/kg


±12 μg/l (100 μg/kg)

CCα = 110 μg/kg

CCβ = 120 μg/kg

sulfametazin / sulfametazine (SMZ)

±11 μg/l (100 μg/kg)

CCα = 109 μg/kg

CCβ = 118 μg/kg


±10 μg/l (100 μg/kg)

CCα = 109 μg/kg

CCβ = 117 μg/kg

sulfakinoksalin / sulfaquinoxaline (SQX)

±10 μg/l (100 μg/kg)

CCα = 109 μg/kg

CCβ = 117 μg/kg

sulfisoksazol / sulfisoxazole (SFX)

±11 μg/l (100 μg/kg)

CCα = 109 μg/kg

CCβ = 118 μg/kg


dapson / dapsone (DP)

CCβ = 2,5 µg/kg

DA / YES

DA / YES

DA / YES



method

Določanje sulfonamidov v medu s HPLC –

fluorescenčno detekcijo

Determining sulfonamides in honey by HPLC

– fluorescence detection

med / honey

(5–50) μg/kg

sulfanilamide / sulfanilamide (SAD)


CCα = 8 μg/kg

CCβ = 11 μg/kg



CCα = 8 μg/kg

CCβ = 11 μg/kg

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No



CCα = 9 μg/kg

CCβ = 13 μg/kg

sulfadiazine / sulfadiazine (SDZ)

±3μg/kg (10 μg/kg)

CCα = 6 μg/kg

CCβ = 7 μg/kg

sulfamerazin / sulfamerazine (SRZ))


CCα = 9 μg/kg

CCβ = 13 μg/kg



CCα = 8 μg/kg

CCβ = 12 μg/kg



CCα = 8 μg/kg

CCβ = 10 μg/kg

sulfametizol / sulfamethizole (SMTZ)


CCα = 7 μg/kg

CCβ = 8 μg/kg



CCα = 6 μg/kg

CCβ = 7 μg/kg



CCα = 6 μg/kg

CCβ = 7 μg/kg



CCα = 6 μg/kg

CCβ = 7 μg/kg



method

Določevanje malahitnega in levkomalahitnega

zelenila v ribah s HPLC z UV-VIS in FLD

Detection of malachite and leuco-malachite

green in fish with HPLC withUV-VIS and

FLD

ribe / fish

malahitno zelenilo / malahit green

0,5–10 μg/kg

CCα = 0,6 μg/kg

CCβ = 1,0 μg/kg

± 0,32 μg/kg (2 g/kg)

± 0,44 μg/kg (3 g/kg)

± 0,57 μg/kg (4 g/kg)

leukomalahitno zelenilo / leucomalahit green

0,5–10 μg/kg

CCα = 0,5 μg/kg

CCβ = 0,9 μg/kg

± 0,46 μg/kg (2 g/kg)

± 0,44 μg/kg (3 g/kg)

± 0,36 μg/kg (4 g/kg)

kristal vijolično

LOQ = 1,0 μg/kg

leukokristal vijolično

LOQ = 1,0 μg/kg

briljant zeleno

LOQ = 1,0 μg/kg

DA / YES

Da / Yes

Da / Yes

Ne / No

Ne / No

Ne / No

25. ISO 13730:1996 Modificirana/Modified

Spektrofotometrična metoda določanja

polifosfatov v mesnih izdelkih

Meat and meat products – Determination of

total phosphorus content – Spectrometric

method

SOP 148, verzija 7


P2O5: 1 – 7 g/kg

± 6 %

sušene mesnine / dry meat products

± 7 %

DA / YES

DA / YES


Določanje vsebnosti hidroksiprolina – meso in

mesni izdelki - spektrofotometrična metoda


meso in mesni izdelki / meat and meat products

(0,05–0,5) g/100g

Vzorci z > 0,1 g/100 g hidroksiprolina

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

hydroxyproline content

SOP 164, verzija 3

± 12 %

Vzorci z ≤ 0,1 g/100g hidroksiprolina

± 18 %


Določanje vsebnosti vode v mesu in mesnih

izdelkih - gravimetrična metoda, sušenje


moisture content

SOP 170, verzija 5


(20–80) g/100g

± 1,0 %

sušene mesnine / dry meat products

± 2,3 %

DA / YES

DA / YES


Določanje vsebnosti celokupne maščobe v

mesu in mesnih izdelkih - gravimetrična

metoda, kislinska hidroliza, ekstrakcija


total fat content

SOP 179, verzija 4


(1 – 50) g/100g

Vzorci s vsebnostjo maščobe < 6 g/100 g

± 14 %

Vzorci s vsebnostjo maščobe ≥ 6 g/100 g

± 5 %

DA / YES

29. AOAC official method

1995 16ed 973.31 (Ch

93 p 8-9) Modificirana/Modified

Določanje vsebnosti nitritov v mesnih izdelkih

- Spektrofotometrična metoda

Nitrites in cured meat – Colorimetric method

SOP 2, verzija 7


(5–140) mg/kg

NaNO2:

(5 – 10) mg/kg

± 50 %

(10 – 30) mg/kg

± 20 %

(30 – 140) mg/kg

± 13 %

DA / YES

30. ISO 936:1998

Določanje vsebnosti celokupnega pepela v

mesu in mesnih izdelkih - gravimetrična, sežig

Meat and meat products -- Determination of

total ash

SOP 281, verzija 2


(1–7,5) g/100g

± 3,6 %

DA / YES


interna metoda

in-house method

Ugotavljanje prisotnosti zaviralnih substanc z

mikrobiološko metodo

Detection of antimicrobial residues with

microbiological method

mleko / milk

tetraciklini / tetracyclines

tetraciklin / tetracycline (TC)

CCβ = 100 µg/kg

klortetraciklin / chlortetracycline (CTC) CCβ = 80

µg/kg

oksitetraciklin / oxytetracycline (OTC)

CCβ = 100 µg/kg

doksiciklin / doxycycline (DC)

CCβ = 30 µg/kg

aminoglikozidi / aminoglycosides

streptomicin / streptomycin

CCβ = 200 µg/kg

dihidrostreptomicin / dihydrostreptomycin

CCβ = 200 µg/kg

gentamicin / gentamycin

CCβ = 100 µg/kg

neomicin / neomycin

CCβ = 100 µg/kg

rifaksimin / rifaximin

CCβ = 50 µg/kg

kanamicin / kanamycin

CCβ = 300 µg/kg (very low sensitivity)

meso / meat



CCβ = 50 µg/kg


µg/kg


CCβ = 70 µg/kg


CCβ = 30 µg/kg

aminoglikozidi / aminoglycosides

apramicin / apramycin

CCβ = 800 µg/kg


CCβ = 500 µg/kg

DA / YES

Da / Yes

Da / Yes

DA / YES

Da / Yes

Da / Yes





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No


CCβ = 500 µg/kg


CCβ = 100 µg/kg

neomicin / neomycin

CCβ = 250 µg/kg


CCβ = 150 µg/kg


CCβ = 200 µg/kg

paramomicin / paramomycin

CCβ = 250 µg/kg

jajca / eggs



CCβ = 200 µg/kg


µg/kg


CCβ = 200 µg/kg


CCβ = 80 µg/kg

NE / NO

Ne/ No


interna metoda

in-house method

Preiskava na kinolone z mikrobiološko

metodo

Microbiological screening method for

quinolones

jajca / eggs:


CCβ = 10 µg/kg


marbofloksacin / marbofloxacin (MAR) sarafloksacin

/ sarafloxacin (SAR)

CCβ = 15 µg/kg


CCβ = 25 µg/kg


CCβ = 60 µg/kg


oksolinska kislina / oxolinic acid (OXO) CCβ = 300

µg/kg

meso / meat:

difloksacin / difloxacin (DIF) enrofloksacin /

enrofloxacin (EFC)


oksolinska kislina / oxolinic acid (OXO) orbifloksacin

/ orbifloxacin (ORB)

CCβ = 50 µg/kg




CCβ = 100 µg/kg

DA / YES

DA / YES

33. ISO 6888-2:1999

Amendment 1: 2003

Preiskava na koagulaza pozitivne stafilokoke

Microbiology of food and animal feeding

stuffs – Horizontal method for the

enumeration of coagulase-positive

staphylococci (S. aureus and other species) by

– Part 2: Technique using rabbit plasma

fibrinogen agar medium

SOP 204, verzija 2


LOD 10 CFU/g

± 0,12 log10 CFU/g

mleko in mlečni izdelki / milk and milk products

LOD 10 CFU/g (ml)

± 0,19 log10 CFU/g

DA / YES

DA / YES

34. ISO 4833-1: 2013

Določanje skupnega števila mikroorganizmov

(tehnika štetja kolonij na ploščah inkubiranih

pri 30 oC)

Microbiology of the food chain – Horizontal

method for the enumeration of

microorganisms Part 1: Colony count at 30 oC

by the pour plate technique

SOP 206, verzija 4


LOD = 10 CFU/g

±0,13 log10 CFU/g


LOD = 10 CFU/g (ml)

±0,08 log10 CFU/g

jajca in jajčni izdelki / eggs and eggs products

LOD = 10 CFU/g (ml)

±0,17 log10 CFU/g (ml)

brisi okolja / environmental swabs

LOD = 5 CFU/površina

±0,17 log10

DA / YES

DA / YES

DA / YES

DA/YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

35. ISO 6579-1:2017

Mikrobiologija v prehranski verigi -

Horizontalna metoda za ugotavljanje

prisotnosti, števila in serotipov Salmonella –

1. del: Ugotavljanje prisotnosti Salmonella

spp.

Microbiology of the food chain - Horizontal

method for the detection and enumeration of

Salmonella spp.

SOP 400, verzija 2/version 2


LOD = (1–5) CFU/25g


LOD = (1–5) CFU/25ml

jajca in jajčni izdelki / eggs and egg products

LOD = (5–10) CFU/25 g

DA / YES

DA / YES

DA / YES

36. ISO 11290-1: 2017



prisotnosti in števila Listeria monocytogenes

in Listeria spp.- 1. del: Ugotavljanje

prisotnosti



Listeria monocytogenes and Listeria spp.-

Part 1: Detection method

SOP 162, verzija 5


LOD = 5 CFU/25 g


LOD = 5 CFU/25g(ml)


LOD = 5-10 CFU/površina

DA / YES

DA / YES

DA / YES

37. ISO 10272-1: 2017

Postopek A

Procedure A



prisotnosti in števila Campylobacter spp. -

1.del: Metoda za ugotavljanje prisotnosti


method for detection and enumeration of

Campylobacter spp.


SOP 187, verzija 4


LOD = 5–10 CFU/10g

mleko / milk

LOD = 5-10 CFU/10g

DA / YES

DA / YES

38. ISO 10272-2 : 2017



prisotnosti in števila Campylobacter spp.-

2.del: Tehnika štetja kolonij



Campylobacter spp.

- Part 2 : Colony-count technique

SOP 385 verzija 3



LOD = 10 CFU/g

± 0,28 log10 CFU/g

DA / YES

39. ISO 11290-2: 2017




in Listeria spp.- 2.del: Ugotavljanje števila



Listeria monocytogenes and of Listeria spp. -

Part 2: Enumeration method

SOP 314, verzija 3


LOD = 10 CFU/g

± 0,19 log10 CFU/g


LOD = 10 CFU/g (ml)

±0,13 log10 CFU/g

DA / YES

DA / YES

40. ISO 16649-2: 2001 Določanje števila β glukuronidaza pozitivnih

bakterij Escherichia coli

Kvantitativna metoda


stuffs - Horizontal method for the enumeration

of β glukuronidase-positive Escherichia coli

Part 2: Colony count technique at 44oC

SOP 315, verzija 2


LOD = 10 CFU/g

±0,18log10CFU/g


LOD = 10 CFU/g (ml)

±0,24 log10 CFU/g (tekočine,liquids)

±0,30 log10 CFU/g (trdne snovi, solid milk products)

DA / YES

DA / YES

41. ISO 16649-3: 2015

Določanje števila β–glucuronidaza-

pozitivnih bakterij Escherichia coli z uporabo

tehnike MPN in 5-bromo-4-chloro-3-indolyl

β–D-glucuronida

Microbiology of food chain - Horizontal

method for the enumeration of β

glucuronidase-positive Escherichia coli

Part 3: Most probable number technique using

5-bromo-4-chloro-3-indolyl β-D-glucuronide

SOP 192, verzija 3


LOD = 18 CFU/100 ml

±0,60 log10 CFU/100 g

DA / YES

42. ISO 21528-2:2017 Mikrobiologija v prehranski verigi -


prisotnost in števila enterobakterij - 2.del:

Metoda štetja kolonij



Enterobacteriaceae - Part 2: Colony count

technique


LOD = 10 CFU/g

±0,13 log10 CFU/g


LOD = 10 CFU/g (ml)

±0,13 log10 CFU/g (tekočine in prašnate snovi / liquids

and powders)

±0,21 log10 CFU/g (trdne snovi / solid milk products)

DA / YES

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

SOP 317, verzija 3

jajca in jajčni izdelki / eggs and eggs products

LOD = 10 CFU/g (ml)

±0,17 log10 CFU/g

hrana za domače živali / pet food and feed

LOD = 10 CFU/g (ml)

±0,17 log10 CFU/g

DA / YES

DA / YES



method

Ugotavljanje zaviralnih substanc v mleku -

DELVOTEST®SP-NT

Determination of antimicrobial substances in

milk - DELVOTEST®SP-NT

mleko / milk

penicilin G / penicillin G

CCβ = 3 µg/kg

ampicilin / ampicillin

CCβ = 4 µg/kg

amoksicilin / amoxicillin

CCβ = 4 µg/kg

kloksacilin / cloxacillin

CCβ = 20 µg/kg

nafcilin / nafcillin

not sensitive

cefapirin / cefapirin

not sensitive

cefkvinom / cefquinome

not sensitive

cefalonij / cefalonium

CCβ = 20 µg/kg

cefoperazon / cefoperazone

CCβ = 50 µg/kg

ceftiofur / ceftiofur

CCβ = 50 µg/kg

cefazolin / cefazolin

CCβ = 10 µg/kg

cefaleksin / cefalexin

CCβ = 70 µg/kg

tetraciklin / tetracycline

very low sensitivity

oksitetraciklin / oxytetracycline


klortetraciklin / chlortetracycline

not sensitive

doksiciklin / doxycycline

not sensitive

neomicin / neomycin

CCβ = 500 µg/kg





gentamicin / gentamicin

not sensitive


not sensitive

kinoloni / quinolones

not sensitive

sulfatiazol / sulfathiazole

CCβ = 90 µg/kg

sulfadiazin / sulfadiazine


sulfadoksin / sulfadoxin


eritromicin / erythromycin


linkomicin / lincomycin


novobiocin / novobiocin




spiramicin / spiramycin


tilozin / tylosin

CCβ = 50 µg/kg

bacitracin / bacitracin


DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No


interna metoda

in-house method

Določanje ostankov bazičnih NSAIDov

(MAA, A, AA, AAA, DMAA, FAA, IPAA, 4-

OH-A) z LC-MS/MS

Determination of basic NSAIDs (MAA, A,

AA, AAA, DMAA, FAA, IPAA, 4-OH-A)

with LC-MS/MS

mleko / milk

(25–250) μg/kg

metilaminoantipirin / methylaminoantipyrine

(MAA)

CCα = 56 μg/kg

CCβ = 61 μg/kg

±6,3 μg/kg (25 μg/kg)

±8,2 μg/kg (50 μg/kg)

±15,5 μg/kg (75 μg/kg)

antipirin / antipyrine (A)

CCα = 55 μg/kg

CCβ = 59 μg/kg

±12,2 μg/kg (25 μg/kg)

±8,3 μg/kg (50 μg/kg)

±15,6 μg/kg (75 μg/kg)

4-aminoantipirin / 4-aminoantipyrine (AA)

CCα = 54 μg/kg

CCβ = 58 μg/kg

±7,8 μg/kg (25 μg/kg)

±6,4 μg/kg (50 μg/kg)

±9,2 μg/kg (75 μg/kg)

4-acetamidoantipirin / 4-acetamidoantipyrine

(AAA)

CCα = 56 μg/kg

CCβ = 61 μg/kg

±6,3 μg/kg (25 μg/kg)

±8,2 μg/kg (50 μg/kg)

±15,5 μg/kg (75 μg/kg)

4-dimetilaminoantipirin / 4-dimethylaminoantipyrine

(DMAA)

CCα = 53 μg/kg

CCβ = 56 μg/kg

±4,7 μg/kg (25 μg/kg)

±5,1 μg/kg (50 μg/kg)

±10,1 μg/kg (75 μg/kg)

4-formilaminoantipirin / 4-formylaminoantipyrine

(FAA)

CCα = 54 μg/kg

CCβ = 59 μg/kg

±12,3 μg/kg (25 μg/kg)

±5,4 μg/kg (50 μg/kg)

±13,9 μg/kg (75 μg/kg)

isopropilaminoantipirin / isopropylaminoantipyrine

(IPAA)

CCα = 52 μg/kg

CCβ = 54 μg/kg

±4,3 μg/kg (25 μg/kg)

±3,7 μg/kg (50 μg/kg)

±8,0 μg/kg (75 μg/kg)


mleko / milk

metilaminoantipirin / methylaminoantipyrine

(MAA)

antipirin / antipyrine (A)

4-aminoantipirin / 4-aminoantipyrine (AA)

4-acetamidoantipirin / 4-acetamidoantipyrine

(AAA)

4-dimetilaminoantipirin / 4-dimethylaminoantipyrine

(DMAA)

4-formilaminoantipirin / 4-formylaminoantipyrine

(FAA)

isopropilaminoantipirin / isopropylaminoantipyrine

(IPAA)

CCβ = 25 μg/kg

DA / YES

DA / YES

45. Intrna metoda

In-house method

Določanje vsebnosti natrija s plamensko

atomsko emisijsko spektrometrijo

Determination of sodium with flame atomic

emission spectroscopy

Živila / food

LOQ 0,01 g/100 g

NE/NO

46. SOP 169

Izvedbena Uredba

Določanje celokupnega hlapnega bazičnega

dušika (TVB-N) v ribah in ribiških proizvodih

ribe in ribiški proizvodi / fish and fishery products

TVB-N: (5 – 100) mg/100 g

NE/NO





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

Komisije (EU) 2019/627

Commission

Implementing

Regulation (EU)

2019/627

Referenčna metoda

Determination of the TVB-N in fish and

fishery products – reference method

47. ISO 2917 Merjenje pH - meso in mesni izdelki

Measurement of pH – meat and meat products

SOP 191

Meso in mesni izdelki /meat and meat products

NE/NO

48. SOP 299

Interna metoda

In-house method

Določevanje prostih maščobnih kislin

Determination of acid value and acidity

rastlinska in živalska maščoba in olja / animal and

vegetable fats and oils

NE/NO

49. ISO 3960 Določanje peroksidnega števila

Determination of peroxide value

SOP 301

rastlinska in živalska maščoba in olja / animal and

vegetable fats and oils

NE/NO

50. Interna metoda

In-house method

Določanje kovin v živilih z ICP-MS

Determination of elements with ICP-MS

živila / food

NE/NO

51. SOP 456

Interna metoda

In-house method

Določanje HMF v medu, glukozno-

fruktoznem sirupu in hrani za čebele

Determination of HMF in honeylucose-

fructose syrup and bee feed

med in sirup / honey

(1,0 -1000) mg/kg

LOD 0,5 mg/kg

LOQ 1,0 mg/kg

± 10 % (1-50 mg/kg)

± 4 % (50–1000 mg/kg)

hrana za čebele / Bee feed

LOD 0,5 mg/kg

LOQ 1,0 mg/kg

± 10 % (1-50 mg/kg)

± 6 % (50–1000 mg/kg)

NE/NO

52. SOP 476

Interna metoda

In-house method

Določanje vsebnosti maščobnih kislin v

živilih GC-FID

Determination of fatty acids in food GC-FID

Živila / food

LOD 0,05 %

NE/NO

53. SOP 376

interna metoda

in-house method

Ugotavljanje prisotnosti streptomicina z

ELISA metodo, presejalna metoda

Determining of Streptomycin presence by

ELISA method, screening method

med / honey

streptomicin / Streptomicin

dihidrostreptomicin/Dihydrostreptomicin

LOD = 10 μg/kg

NE/ NO

54. SOP 444

interna metoda

in-house method

Določanje nizkomolekularnih sladkorjev v

živilih s HPTLC

Determination of low molecular weigt

carbohidrates in food with HPTLC

živila / food

saharoza / saccharose:

(0,05–5) g/100 g

fruktoza / fructose:

(0,05–5) g/100 g

maltoza / maltose:

(0,05–5) g/100 g

laktoza / lactose:

(0,05–5) g/100 g

glukoza / glucose:

(0,05–5) g/100 g

NE / NO

55. IDF 21B: 1987

Določanje vsebnosti skupne suhe snovi v

mleku

Milk, cream and evaporated milk:

Determination of total solid content (reference

method)

SOP 182, verzija 2

mleko / milk

(0–20) g/100g

±0,11 g/100g

NE / NO

56. SOP 391

interna metoda

in-house method

Preiskava na tetraciklinske in beta-laktamske

antibiotike v mleku s testom TWINSENSOR

Screening method for tetracyclines and beta-

lactams antibiotics in milk - TWINSENSOR

mleko / milk


CCβ = 4 µg/kg


CCβ = 4 µg/kg

amoksicilin / amoksicillin

CCβ = 4 µg/kg


CCβ = 20 µg/kg


CCβ = 60 µg/kg


CCβ = 60 µg/kg


CCβ = 20 µg/kg


CCβ = 20 µg/kg


NE / NO





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

CCβ = 50 µg/kg


CCβ = 50 µg/kg


CCβ = 50 µg/kg


CCβ = 100 µg/kg

klortetraciklin / chlortetracycline (CTC)

CCβ = 100 µg/kg

oksitetraciklin / oxytetracycline (OTC) CCβ = 100

µg/kg


CCβ = 30 µg/kg

dihidrostreptomicin / dihydrostreptomicine

not sensitive


not sensitive


not sensitive

neomicin / neomycin

not sensitive

streptomicin / streptomycine



not sensitive


not sensitive


not sensitive


not sensitive

sulfadimidin / sulfadimidine

not sensitive


not sensitive


not sensitive


not sensitive


not sensitive

tilozin / tylosin

not sensitive

trimetoprim / trimitroprim


57. SOP 421

interna metoda

in-house method

Preiskava na tetraciklinske antibiotike v medu

s testom TETRASENSOR

Screening method for tetracyclines antibiotics

in honey - TETRASENSOR

med / honey


CCβ = 15 µg/kg


CCβ = 10 µg/kg


CCβ = 15 µg/kg


CCβ = 10 µg/kg

NE / NO

58. SOP 312

interna metoda

in-house method

Ugotavljanje prisotnosti lipofilnih toksinov v

školjkah z biološkim poskusom

Determination of lipophilic toxins in shellfish

by biological test


okadajična kislina/okadaic acid

LOD = 160 μg/kg

NE / NO

59. AOAC 959.08

Official Method

Ugotavljanje prisotnosti PSP toksina v

školjkah z biološkim poskusom

Determination of PSP toxins in shellfish by

biological test

SOP 313


LOQ = 800 μg/kg

NE / NO

60. ISO 7932:2005 Določanje števila bakterij Bacillus cereus

Horizontal method for the enumeration of

presumptive Bacillus cereus


LOD = 10 CFU/g (ml)

±0,26 log10 CFU/g


LOD = 10 CFU/g (ml)

±0,26 log10 CFU/g

NE / NO





Št.

No.



number

Preskusna metoda

Test method





Akreditirano*

Da/Ne

Accredited Yes/No

61. ISO 10273:2003

Modifikacija

/ modification

Ugotavljanje prisotnosti bakterije Yersinia

enterocolitica

Horizontal method for the detection Yersinia

enterocolitica




NE / NO

62. ISO 15213 Ugotavljanje prisotnosti in števila sulfit

reducirajoči klostridijev, ki rastejo v

anaerobnih pogojih

Horizontal method for the enumeration of sulfite-

reducing bacteria growing under anaerobic

conditions

Živila, voda / Food, water NE / NO

63. ISO 15216-2 Ugotavljanje prisotnosti virusa hepatitisa A z

uporabo PCR v realnem času

Kvalitativna metoda

Horizontal method for determination of hepatitis A

virus using real-time RT-PCR Part 2: Method for detection

Živila (školjke) / Food (shellfish) NE / NO

64. ISO 15216-2 Ugotavljanje prisotnosti norovirusov z

uporabo PCR v realnem času

Kvalitativna metoda

Horizontal method for determination of

norovirus using real-time RT-PCR

Part 2: Method for detection

Živila (školjke) / Food (shellfish) NE / NO

65. ISO 21807 Določanje aktivnosti vode

Determination of water activity

Živila / Food NE / NO

66. ISO 21872-1 Ugotavljanje prisotnosti bakterije Vibrio

parahaemolyticus

Horizontal method for detection of Vibrio

parahaemolyticus


67. Evropska izločilna

metoda EU-RL za

koagulaza pozitivne

stafilokoke

European screening

method of the EU-RL for

CPS

Ugotavljanje prisotnosti stafilokoknih

enterotoksinov tipa A-E

Detection of staphylococcal enterotoxins types

SEA-SEE


68. interna metoda

in-house method

Ugotavljanje prisotnosti bakterije Clostridium

difficile z uporabo PCR v realnem času

Horizontal method for detection of Clostridium

difficile using real-time RT-PCR

Meso in mesni izdelki / Meat and meat products

LOD ≥ 5 CFU

NE / NO

69. interna metoda

in-house method

Ugotavljanje prisotnosti bakterije Clostridium

difficile – izolacija

Horizontal method for detection of Clostridium

difficile - isolation

Meso in mesni izdelki / Meat and meat products

LOD ≥ 5 CFU

NE / NO

70. ISO 17410 Ugotavljanje števila psihrotropnih

mikroorganizmov Horizontal method for the enumeration of

psychrotropic microorganisms

Živila / Food

LOD ≥ 10 CFU

NE / NO

71. ISO 15214 Ugotavljanje števila mezofilnih

mlečnokislinskih bakterij – štetje na ploščah

pri 30 °C Horizontal method for the enumeration of mesophilic

lactic acid bacteria – Colony-count technique at 30

°C

Živila / Food

LOD ≥ 10 CFU

NE / NO

72. interna metoda

in-house method

Ugotavljanje števila plesni in kvasovk

Horizontal method for the enumeration of molds and

yeasts

Živila / Food

LOD ≥ 100 CFU

NE / NO

73. interna metoda

in-house method

Ugotavljanje števila anaerobnih bakterij

- štetje na ploščah pri 30 °C

Horizontal method for the enumeration of anaerobic

bacteria – Colony-count technique at 30 °C

Živila / Food

LOD ≥ 10 CFU

NE / NO

74. interna metoda

in-house method

Ugotavljanje števila sporogenih bakterij

- štetje na ploščah pri 30 °C

Horizontal method for the enumeration of sporogenic

bacteria – Colony-count technique at 30 °C

Živila / Food

LOD ≥ 10 CFU

NE / NO

75. interna metoda

in-house method

Ugotavljanje prisotnosti tkiv centralnega

živčnega sistema - ELISA

Detection of central nervous system tissue - ELISA

Brisi / Swabs NE / NO




Inštitut za varno hrano, krmo in okolje; Enota za okolje, prehrano, dobrobit in higieno živali

Št.

No.


Internal/standard reference number

Preskusna metoda

Test method

Materiali, proizvodi; Merilna negotovost (podana kot razširjena merilna

negotovost U); Meje metode

Material, products; Measurement uncertainty(expressed as expanded


Akreditirano* Da/Ne

Accredited Yes/No


Interna metoda /

In-house method

Določanje aflatoksina M1 v mleku in

mlečnih izdelkih; HPLC-fluorescenčna

detekcija

Determination of aflatoxin M1 in milk and

milk products; HPLC-fluorescence

Mleko / Milk

0,015–1 g/kg

LOD = 0,005 g/kg

LOQ = 0,015 g/kg

0,004 g/kg (0,015 g/kg)

0,012 g/kg (0,050 g/kg)

0,24 g/kg (1 g/kg)

Mleko v prahu / Milk powder

0,15–10 g/kg

LOD = 0,05 g/kg

LOQ = 0,15 g/kg

0,12 g/kg (0,50 g/kg)

Sir, sladoled, smetana, jogurt /

Cheese, ice-cream, cream, yoghurt

0,045–0,500 g/kg

LOD = 0,015 g/kg

LOQ = 0,045 g/kg

0,022 g/kg (0,050 g/kg )

DA / YES

DA / YES

NE / NO


Interna metoda /

In-house method

Določanje aflatoksina B1 v živilih živalskega

izvora; HPLC-fluorescenčna detekcija

Determination of aflatoxin B1 in food of

animal origin; HPLC-fluorescence

Jetra / Liver

15–75 ng/kg

LOD = 5 ng/kg

LOQ = 15 ng/kg

7 ng/kg (25 ng/kg)

16 ng/kg (50 ng/kg)

23 ng/kg (75 ng/kg)

Jajca / Eggs

6–50 ng/kg

LOD = 2 ng/kg

LOQ = 6 ng/kg

10 ng/kg (50 ng/kg )

DA / YES

DA / YES


Interna metoda /

In-house method

Določanje ohratoksina A v živilih

živalskega izvora; HPLC-fluorescenčna

detekcija

Determination of ochratoxin A in food of

animal origin; HPLC-fluorescence

Ledvice / Kidneys

2,5–7,5 g/kg

LOD = 0,8 g/kg

LOQ = 2,5 g/kg

0,21 g/kg (2,5 g/kg)

0,51 g/kg (5,0 g/kg)

0,62 g/kg (7,5 g/kg)

DA / YES


Interna metoda /

In-house method

Določanje aflatoksinov v krmi in sestavinah

krme; HPLC-fluorescenčna detekcija

Determination of aflatoxins in feed and feed

ingredients; HPLC-fluorescence

Krma / Feed

Aflatoksin B1 / Aflatoxin B1

0,0006–0,030 mg/kg

LOD = 0,0002 mg/kg

LOQ = 0,0006 mg/kg

30 % g/kg (0,0006–0,030 mg/kg)

Aflatoksin B2, G1, G2 / Aflatoxin B2,G1,G2

0,001–0,010 mg/kg

LOD = 0,0004 mg/kg

LOQ = 0,001 mg/kg

30 % (0,001–0,010 mg/kg)

DA / YES

Da / Yes

Ne / No


Interna metoda /

In-house method

Določanje ohratoksina A v krmi in

sestavinah krme; HPLC-fluorescenčna

detekcija

Determination of ochratoxin A in feed and

feed ingredients; HPLC-fluorescence

Krma / Feed

0,03–0,3 mg/kg

LOD = 0,01 mg/kg

LOQ = 0,03 mg/kg

0,023 mg/kg (0,1 mg/kg)

0,047 mg/kg (0,2 mg/kg)

0,072 mg/kg (0,3 mg/kg)

DA / YES


Interna metoda /

In-house method

Določanje zearalenona v krmi in sestavinah

krme; HPLC-fluorescenčna detekcija

Determination of zearalenone in feed and

feed ingredients; HPLC-fluorescence

Krma / Feed

0,05–0,3 mg/kg

LOD = 0,02 mg/kg

LOQ = 0,05 mg/kg

0,017 mg/kg (0,1 mg/kg)

0,037 mg/kg (0,2 mg/kg)

0,052 mg/kg (0,3 mg/kg)

DA / YES

82. ISO 14183:2005

Določanje monenzina, narazina and

salinomicina v krmi, premiksih in

Krma / Feed

Premiksi / Premixes

DA / YES

DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

koncentratih; HPLC-UV detekcija s

pokolonsko derivatizacijo

Determination of monensin, narasin and

salinomycin in feed, premixes and

concentrates; HPLC- UV detection with

post-column derivatization

SOP 272, verzija 4

Koncentrati / Concentrates

Monenzin / Monensin

1 mg/kg–180 g/kg

LOD = 0,3 mg/kg

LOQ = 1 mg/kg

0,1 mg/kg (0,9 mg/kg)

3,1 mg/kg (90 mg/kg)

2,3 g/kg (18 g/kg)

1,7 % (180 g)

Salinomicin / Salinomycin

2 mg/kg–120 g/kg

LOD = 0,5 mg/kg

LOQ = 2 mg/kg

0,2 mg/kg (1,7 mg/kg)


1,7 g/kg (12 g/kg)

1,2 % (120 g)

Krma / Feed

Narazin / Narasin

LOD = 0,5 mg/kg

LOQ = 2 mg/kg


DA / YES

Da /Yes

Da /Yes

DA / YES

Da /Yes


Interna metoda /

In-house method

Določanje semduramicina v krmi, premiksih

in koncentratih; HPLC-UV/VIS detekcija s

pokolonsko derivatizacijo

Determination of semduramicin in feed,

premixes and concentrates; HPLC- UV/VIS

detection with post-column derivatization

Premiksi, koncentrati / Premixes, concentrates

0,1–50 g/kg

LOD = 0,04 g/kg

LOQ = 0,1 g/kg

0,2 g/kg (2,5 g/kg)

0,7 % (50 g)

DA / YES


Interna metoda /

In-house method

Določanje fumonizinov B1 in B2 v krmi in

sestavinah krme; HPLC-fluorescenčna

detekcija

Determination of fumonisins B1 and B2 in

feed and feed ingredients; HPLC-

fluorescence

Koruza, krma na osnovi koruze / Maize, maize based

feed

Fumonizin B1 / Fumonisin B1

0,3–1,7 mg/kg

LOD = 0,1 mg/kg

LOQ = 0,3 mg/kg

25 % (0,3–1,7 mg/kg)


0,3–0,5 mg/kg

LOD = 0,1 mg/kg

LOQ = 0,3 mg/kg

25 % (0,3–0,5 mg/kg)

NE / NE

Ne /No

Ne /No

85. SOP 501

interna metoda

In-house method

Mikotoksini - Ekstrakcija / tekočinska

kromatografija z masno selektivnim

detektorjem (LC-MS/MS)

Mycotoxins - Extraction / liquid

chromatography mass spectrometry (LC-

MS/MS)

Žita, krmne mešanice / Grains, compound feed


0,05–0,5 mg/kg

LOD = 0,015 mg/kg

LOQ = 0,05 mg/kg

0,014 mg/kg (0,05 mg/kg)

0,019 mg/kg (0,10 mg/kg)

0,329 mg/kg (1,0 mg/kg)


0,05–0,5 mg/kg

LOD = 0,015 mg/kg

LOQ = 0,05 mg/kg

0,023 mg/kg (0,05 mg/kg)

0,040 mg/kg (0,10 mg/kg)

0,344 mg/kg (1,0 mg/kg)

NE / NE

Ne /No

Ne /No

86. Uredba Komisije (ES) št.

152/2009, Priloga III,

Metoda A

Commission Regulation

(EC) No 152/2009,

Annex III, Method A

Določanje vlage s tehtanjem

Determination of moisture by gravimetry

SOP 250, verzija 4

Krmne mešanice, sestavine krme, premiksi, dodatki

krmi / Compound feed, feed material, premixtures, feed

additives

6,50–83 %

DA / YES


Interna metoda /

In-house method

Določanje trihotecenov tipa A in B v krmi in

sestavinah krme; GC-MS po derivatizaciji

Determination of type A and type B

trichothecenes in feed and feed ingredients;

GC-MS after derivatization

Krma / Feed

0,1–1,0 mg/kg

LOD = 0,05 mg/kg

LOQ = 0,1 mg/kg

Deoksinivalenol / Deoxynivalenol (DON)

DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

0,09 mg/kg (0,3 mg/kg)

0,17 mg/kg (0,5 mg/kg)

0,33 mg/kg (1,0 mg/kg)

3-Acetildeoksinivalenol / 3-Acetyldeoxynivalenol (3-

AcDON)

0,08 mg/kg (0,3 mg/kg)

0,16 mg/kg (0,5 mg/kg)

0,35 mg/kg (1,0 mg/kg)

Diacetoksiscirpenol / Diacetoxyscirpenol (DAS)

0,07 mg/kg (0,3 mg/kg)

0,12 mg/kg (0,5 mg/kg)

0,23 mg/kg (1,0 mg/kg)

15-Acetildeoksinivalenol / 15-Acetyldeoxynivalenol

(15-AcDON)

0,07 mg/kg (0,3 mg/kg)

0,15 mg/kg (0,5 mg/kg)

0,25 mg/kg (1,0 mg/kg)

Nivalenol / Nivalenol (NIV)

0,08 mg/kg (0,3 mg/kg)

0,18 mg/kg (0,5 mg/kg)

0,27 mg/kg (1,0 mg/kg)

Neosolaniol / Neosolaniol (NSOL)

0,07 mg/kg (0,3 mg/kg)

0,13 mg/kg (0,5 mg/kg)

0,24 mg/kg (1,0 mg/kg)

HT-2

0,07 mg/kg (0,3 mg/kg)

0,18 mg/kg (0,5 mg/kg)

0,26 mg/kg (1,0 mg/kg)

T-2

0,07 mg/kg (0,3 mg/kg)

0,14 mg/kg (0,5 mg/kg)

0,25 mg/kg (1,0 mg/kg)


Interna metoda /

In-house method

Določanje elementov v krmi; ICP - MS

Determination of elements in feed; ICP-MS

Krma / Feed

Mn

3,53−9500 mg/kg

LOD = 1,06 mg/kg

LOQ = 3,53 mg/kg

7,60 mg/kg (65,4 mg/kg)

7,62 mg/kg (71,7 mg/kg)

Fe

6,62−5800 mg/kg

LOD = 1,98 mg/kg

LOQ = 6,62 mg/kg

20,4 mg/kg (180 mg/kg)

21,0 mg/kg (278 mg/kg)

162 mg/kg (957 mg/kg)

Co

0,008−19 mg/kg

LOD = 0,002 mg/kg

LOQ = 0,008 mg/kg

0,028 mg/kg (0,216 mg/kg)

0,029 mg/kg (0,355 mg/kg)

Cu

0,65−1200 mg/kg

LOD = 0,19 mg/kg

LOQ = 0,65 mg/kg

0,79 mg/kg (7,24 mg/kg)

1,37 mg/kg (9,8 mg/kg)

1,23 mg/kg (10,3 mg/kg)

Zn

1,05−7200 mg/kg

LOD = 0,31 mg/kg

LOQ = 1,05 mg/kg

DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

2,22 mg/kg (24,4 mg/kg)

2,63 mg/kg (26,7 mg/kg)

4,45 mg/kg (66,7 mg/kg)

Mo

0,004−7 mg/kg

LOD = 0,001 mg/kg

LOQ = 0,004 mg/kg

0,59 mg/kg (0,78 mg/kg)

0,70 mg/kg (1,85 mg/kg)

0,34 mg/kg (2,36 mg/kg)

Cd

0,005−2,89 mg/kg

LOD = 0,002 mg/kg

LOQ = 0,005 mg/kg

0,004 mg/kg (0,104 mg/kg)

0,30 mg/kg (1,78 mg/kg)

0,11 mg/kg (2,58 mg/kg)

Hg

0,025−1,88 mg/kg

LOD = 0,008 mg/kg

LOQ = 0,025 mg/kg

0,007 mg/kg (0,023 mg/kg)

0,38 mg/kg (1,88 mg/kg)

Pb

0,015−5,65 mg/kg

LOD = 0,005 mg/kg

LOQ = 0,015 mg/kg

0,03 mg/kg (0,19 mg/kg)

0,14 mg/kg (1,67 mg/kg)

0,31 mg/kg (1,81 mg/kg)

As

0,026−2,82 mg/kg

LOD = 0,008 mg/kg

LOQ = 0,026 mg/kg

0,010 mg/kg (0,044 mg/kg)

0,017 mg/kg (0,076 mg/kg)

0,40 mg/kg (1,94 mg/kg)

Se

0,033−44 mg/kg

LOD = 0,010 mg/kg

LOQ = 0,033 mg/kg

0,012 mg/kg (0,032 mg/kg)

0,02 mg/kg (0,12 mg/kg)

0,28 mg/kg (1,94 mg/kg)


Interna metoda /

In-house method

Določanje kokcidiostatikov v krmi za

neciljne živali; LC-MS/MS

Determination of coccidiostats in non-target

feed; LC-MS/MS

Krma / Feed

Natrijev lasalocid / Lasalocid sodium

0,125−12,5 mg/kg

LOD = 0,04 mg/kg

LOQ = 0,125 mg/kg

0,29 mg/kg (1,25 mg/kg)

0,31 mg/kg (3,75 mg/kg)

Narazin / Narasin

0,07−7,0 mg/kg

LOD = 0,02 mg/kg

LOQ = 0,07 mg/kg

0,18 mg/kg (0,7 mg/kg)

0,37 mg/kg (2,1 mg/kg)

Natrijev salinomicin / Salinomycin sodium

0,07−7,0 mg/kg

LOD = 0,02 mg/kg

LOQ = 0,07 mg/kg

0,14 mg/kg (0,7 mg/kg)

0,38 mg/kg (2,1 mg/kg)

Natrijev monenzin / Monensin sodium

0,125–12,5 mg/kg

LOD = 0,04 mg/kg

LOQ = 0,125 mg/kg

0,26 mg/kg (1,25 mg/kg)

DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

0,76 mg/kg (3,75 mg/kg)

Natrijev semduramicin / Semduramicin sodium

0,025–2,5 mg/kg

LOD = 0,01 mg/kg

LOQ = 0,025 mg/kg

0,11 mg/kg (0,25 mg/kg)

0,27 mg/kg (0,75 mg/kg)

Amonijev maduramicin / Maduramicin ammonium

0,005–0,5 mg/kg

LOD = 0,002 mg/kg

LOQ = 0,005 mg/kg

0,022 mg/kg (0,05 mg/kg)

0,054 mg/kg (0,15 mg/kg)

Robenidin hidroklorid / Robenidine hydrochloride

0,07–7,0 mg/kg

LOD = 0,02 mg/kg

LOQ = 0,07 mg/kg

0,18 mg/kg (0,7 mg/kg)

0,42 mg/kg (2,1 mg/kg)

Dekokvinat / Decoquinate

0,04–1,77 mg/kg

LOD = 0,01 mg/kg

LOQ = 0,04 mg/kg

0,15 mg/kg (0,4 mg/kg)

0,43 mg/kg (1,2 mg/kg)

Halofuginon hidrobromid / Halofuginone

hydrobromide

0,003–0,3 mg/kg

LOD = 0,001 mg/kg

LOQ = 0,003 mg/kg

0,006 mg/kg (0,03 mg/kg)

0,016 mg/kg (0,09 mg/kg)

Nikarbazin / Nicarbazin

0,05–12,5 mg/kg

LOD = 0,02 mg/kg

LOQ = 0,05 mg/kg

0,12 mg/kg (1,25 mg/kg)

0,20 mg/kg (3,75 mg/kg)

Diklazuril / Diclazuril

0,005–0,1 mg/kg

LOD = 0,002 mg/kg

LOQ = 0,005 mg/kg

0,003 mg/kg (0,01 mg/kg)

0,006 mg/kg (0,03 mg/kg)


Interna metoda /

In-house method

Določanje beta-agonistov v živilih, krmi in

bioloških vzorcih; LC-MS/MS

Determination of beta-agonists; in food, feed

and biological samples; LC-MS/MS

Jetra / Liver


Brombuterol / Brombuterol

0,1–1 µg/kg

CCα = 0,26 μg/kg

CCβ = 0,31 μg/kg

±0,02 μg/kg (0,1 μg/kg)

±0,07 μg/kg (0,2 μg/kg)

±0,11 μg/kg (0,3 μg/kg)

Bromklorbuterol / Bromchlorbuterol

0,1–1 µg/kg

CCα = 0,24 μg/kg

CCβ = 0,27 μg/kg

±0,02 μg/kg (0,1 μg/kg)

±0,04 μg/kg (0,2 μg/kg)

±0,09 μg/kg (0,3 μg/kg)

Cimaterol / Cimaterol

0,25–2,5 µg/kg

CCα = 0,60 μg/kg

CCβ = 0,70 μg/kg

±0,08 μg/kg (0,25 μg/kg)

±0,12 μg/kg (0,5 μg/kg)

±0,19 μg/kg (0,75 μg/kg)

DA / YES

DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

Cimbuterol / Cimbuterol

0,25–2,5 µg/kg

CCα = 0,59 μg/kg

CCβ = 0,68 μg/kg

±0,14 μg/kg (0,25 μg/kg)

±0,11 μg/kg (0,5 μg/kg)

±0,14 μg/kg (0,75 μg/kg)

Klenbuterol / Clenbuterol

0,05–0,5 µg/kg

CCα = 0,12 μg/kg

CCβ = 0,13 μg/kg

±0,02 μg/kg (0,05 μg/kg)

±0,02 μg/kg (0,1 μg/kg)

±0,04 μg/kg (0,15 μg/kg)

Klenciklohekserol / Clencyclohexerol

0,5–5 µg/kg

CCα = 1,53 μg/kg

CCβ = 2,06 μg/kg

±0,57 μg/kg (0,5 μg/kg)

±0,65 μg/kg (1,0 μg/kg)

±0,98 μg/kg (1,5 μg/kg)

Klenhekserol / Clenhexerol

0,5–5 µg/kg

CCα = 1,40 μg/kg

CCβ = 1,79 μg/kg

±0,33 μg/kg (0,5 μg/kg)

±0,48 μg/kg (1,0 μg/kg)

±0,84 μg/kg (1,5 μg/kg)

Klenpenterol / Clenpenterol

0,25–2,5 µg/kg

CCα = 0,72 μg/kg

CCβ = 0,94 μg/kg

±0,13 μg/kg (0,25 μg/kg)

±0,27 μg/kg (0,5 μg/kg)

±0,46 μg/kg (0,75 μg/kg)

Klenproperol / Clenproperol

0,25–2,5 µg/kg

CCα = 0,61 µg/kg

CCβ = 0,73 µg/kg

±0,04 μg/kg (0,25 μg/kg)

±0,14 μg/kg (0,5 μg/kg)

±0,31 μg/kg (0,75 μg/kg)

Fenoterol / Fenoterol

0,5–5 µg/kg

CCα = 1,23 µg/kg

CCβ = 1,47 µg/kg

±0,24 μg/kg (0,5 μg/kg)

±0,28 μg/kg (1,0 μg/kg)

±0,33 μg/kg (1,5 μg/kg)

Izokssuprin / Isoxsuprine

0,25–2,5 µg/kg

CCα = 0,65 µg/kg

CCβ = 0,79 µg/kg

±0,08 μg/kg (0,25 μg/kg)

±0,18 μg/kg (0,5 μg/kg)

±0,16 μg/kg (0,75 μg/kg)

Mabuterol / Mabuterol

0,1–1 µg/kg

CCα = 0,33 µg/kg

CCβ = 0,46 µg/kg

±0,07 μg/kg (0,1 μg/kg)

±0,16 μg/kg (0,2 μg/kg)

±0,17 μg/kg (0,3 μg/kg)

Mapenterol / Mapenterol

0,1–1 µg/kg

CCα = 0,27 µg/kg

CCβ = 0,34 µg/kg

±0,05 μg/kg (0,1 μg/kg)

±0,09 μg/kg (0,2 μg/kg)

±0,14 μg/kg (0,3 μg/kg)





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

Raktopamin / Ractopamine

0,25–2,5 µg/kg

CCα = 0,61 µg/kg

CCβ = 0,72µg/kg

±0,10 μg/kg (0,25 μg/kg)

±0,13 μg/kg (0,5 μg/kg)

±0,14 μg/kg (0,75 μg/kg)

Ritodrin / Ritodrine

0,25–2,5 µg/kg

CCα = 0,69 μg/kg

CCβ = 0,89 μg/kg

±0,09 μg/kg (0,25 μg/kg)

±0,24 μg/kg (0,5 μg/kg)

±0,15 μg/kg (0,75 μg/kg)

Salbutamol / Salbutamol

0,5–5 µg/kg

CCα = 1,07 µg/kg

CCβ = 1,14 µg/kg

±0,07 μg/kg (0,5 μg/kg)

±0,09 μg/kg (1,0 μg/kg)

±0,35 μg/kg (1,5 μg/kg)

Salmeterol / Salmeterol

0,5–5 µg/kg

CCα = 1,28µg/kg

CCβ = 1,56 µg/kg

±0,21 μg/kg (0,5 μg/kg)

±0,34 μg/kg (1,0 μg/kg)

±0,27 μg/kg (1,5 μg/kg)

Terbutalin / Terbutaline

0,5–5 µg/kg

CCα = 1,19 µg/kg

CCβ = 1,37 µg/kg

±0,14 μg/kg (0,5 μg/kg)

±0,23 μg/kg (1,0 μg /kg)

±0,23 μg/kg (1,5 μg /kg)

Tulobuterol / Tulobuterol

0,1–1 µg/kg

CCα = 0,31 µg/kg

CCβ = 0,42 µg/kg

±0,03 μg/kg (0,1 μg/kg)

±0,13 μg/kg (0,2 μg/kg)

±0,14 μg/kg (0,3 μg/kg)

Zilpaterol / Zilpaterol

0,5–5 µg/kg

CCα = 1,21 µg/kg

CCβ = 1,42 µg/kg

±0,25 μg/kg (0,5 μg/kg)

±0,26 μg/kg (1,0 μg/kg)

±0,80 μg/kg (1,5 μg/kg)

Presejalna metoda / Screening method


CCβ = 0,10 µg/kg


CCβ = 0,10 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,05 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,50 μg/kg


CCβ = 0,25 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,25 µg/kg


DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

CCβ = 0,10 µg/kg


CCβ = 0,10 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,10 µg/kg


CCβ = 0,50 µg/kg

Urin / Urine



(0,1–1) µg/kg

CCα = 0,23 μg/kg

CCβ = 0,25 μg/kg

±0,02 μg/kg (0,1 μg /kg)

±0,03 μg/kg (0,2 μg /kg)

±0,04 μg/kg (0,3 μg /kg)


(0,1–1) µg/kg

CCα = 0,23 μg/kg

CCβ = 0,26 μg/kg

±0,02 μg/kg (0,1 μg /kg)

±0,03 μg/kg (0,2 μg /kg)

±0,03 μg/kg (0,3 μg /kg)


(0,25–2,5) µg/kg

CCα = 0,61 μg/kg

CCβ = 0,71 μg/kg

±0,08 μg/kg (0,25 μg /kg)

±0,13 μg/kg (0,5 μg /kg)

±0,19 μg/kg (0,75 μg /kg)


(0,25–2,5) µg/kg

CCα = 0,59 μg/kg

CCβ = 0,67 μg/kg

±0,06 μg/kg (0,25 μg /kg)

±0,11 μg/kg (0,5 μg /kg)

±0,19 μg/kg (0,75 μg /kg)


(0,05–0,5) µg/kg

CCα = 0,11 μg/kg

CCβ = 0,12 μg/kg

±0,01 μg/kg (0,05 μg /kg)

±0,01 μg/kg (0,1 μg /kg)

±0,02 μg/kg (0,15 μg /kg)


(0,5–5,0) µg/kg

CCα = 1,23 μg/kg

CCβ = 1,45 μg/kg

±0,24 μg/kg (0,5 μg /kg)

±0,28 μg/kg (1,0 μg /kg)

±0,51 μg/kg (1,5 μg /kg)


(0,5–5) µg/kg

CCα = 1,51 μg/kg

CCβ = 2,02 μg/kg

±0,23 μg/kg (0,5 μg /kg)

±0,62 μg/kg (1,0 μg /kg)

±0,55 μg/kg (1,5 μg /kg)


(0,25–2,5) µg/kg

CCα = 0,69 μg/kg

CCβ = 0,89 μg/kg

±0,14 μg/kg (0,25 μg /kg)

DA / YES

DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

±0,24 μg/kg (0,5 μg /kg)

±0,61 μg/kg (0,75 μg /kg)


(0,25–2,5) µg/kg

CCα = 0,59 µg/kg

CCβ = 0,69 µg/kg

±0,09 μg/kg (0,25 μg /kg)

±0,11 μg/kg (0,5 μg /kg)

±0,13 μg/kg (0,75 μg /kg)


(0,25–2,5) µg/kg

CCα = 0,62 µg/kg

CCβ = 0,74 µg/kg

±0,09 μg/kg (0,25 μg /kg)

±0,14 μg/kg (0,5 μg /kg)

±0,19 μg/kg (0,75 μg /kg)


(0,1–1) µg/kg

CCα = 0,25 µg/kg

CCβ = 0,30 µg/kg

±0,08 μg/kg (0,1 μg /kg)

±0,06 μg/kg (0,2 μg /kg)

±0,38 μg/kg (0,3 μg /kg)


(0,1–1) µg/kg

CCα = 0,23 µg/kg

CCβ = 0,26 µg/kg

±0,04 μg/kg (0,1 μg /kg)

±0,04 μg/kg (0,2 μg /kg)

±0,08 μg/kg (0,3 μg /kg)


(0,25–2,5) µg/kg

CCα = 0,55 µg/kg

CCβ = 0,61µg/kg

±0,04 μg/kg (0,25 μg /kg)

±0,07 μg/kg (0,5 μg /kg)

±0,24 μg/kg (0,75 μg /kg)


(0,25–2,5) µg/kg

CCα = 0,57 μg/kg

CCβ = 0,65 μg/kg

±0,05 μg/kg (0,25 μg /kg)

±0,09 μg/kg (0,5 μg /kg)

±0,21 μg/kg (0,75 μg /kg)


(0,5–5) µg/kg

CCα = 1,07 µg/kg

CCβ = 1,14 µg/kg

±0,11 μg/kg (0,5 μg /kg)

±0,09 μg/kg (1,0 μg /kg)

±0,23 μg/kg (1,5 μg /kg)


(0,5–5) µg/kg

CCα = 1,17µg/kg

CCβ = 1,34 µg/kg

±0,09 μg/kg (0,5 μg /kg)

±0,21 μg/kg (1,0 μg /kg)

±0,27 μg/kg (1,5 μg /kg)


(0,5–5) µg/kg

CCα = 1,48 µg/kg

CCβ = 1,97 µg/kg

±0,10 μg/kg (0,5 μg /kg)

±0,59 μg/kg (1,0 μg /kg)

±1,29 μg/kg (1,5 μg /kg)


(0,1–1) µg/kg

CCα = 0,32 µg/kg

CCβ = 0,43 µg/kg

±0,08 μg/kg (0,1 μg /kg)

±0,14 μg/kg (0,2 μg /kg)

±0,13 μg/kg (0,3 μg /kg)





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No


(0,5–5) µg/kg

CCα = 1,31 µg/kg

CCβ = 1,61 µg/kg

±0,12 μg/kg (0,5 μg /kg)

±0,37 μg/kg (1,0 μg /kg)

±0,71 μg/kg (1,5 μg /kg)



CCβ = 0,10 µg/kg


CCβ = 0,10 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,05 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,50 μg/kg


CCβ = 0,25 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,10 µg/kg


CCβ = 0,10 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,10 µg/kg


CCβ = 0,50 µg/kg

Mleko / Milk



0,1–1 µg/kg

CCα = 0,26 μg/kg

CCβ = 0,31 μg/kg

±0,02 μg/kg (0,1 μg/kg)

±0,07 μg/kg (0,2 μg/kg)

±0,11 μg/kg (0,3 μg/kg)


0,1–1 µg/kg

CCα = 0,24 μg/kg

CCβ = 0,27 μg/kg

±0,02 μg/kg (0,1 μg/kg)

±0,04 μg/kg (0,2 μg/kg)

±0,09 μg/kg (0,3 μg/kg)


0,25–2,5 µg/kg

CCα = 0,60 μg/kg

CCβ = 0,70 μg/kg

±0,08 μg/kg (0,25 μg/kg)

±0,12 μg/kg (0,5 μg/kg)

±0,19 μg/kg (0,75 μg/kg)


0,1–1 µg/kg

CCα = 0,59 μg/kg

CCβ = 0,68 μg/kg

±0,14 μg/kg (0,25 μg/kg)

DA / YES

DA / YES

DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

±0,11 μg/kg (0,5 μg/kg)

±0,14 μg/kg (0,75 μg/kg)


0,05–0,5 µg/kg

CCα = 0,06 μg/kg

CCβ = 0,08 μg/kg

±0,02 μg/kg (0,05 μg/kg)

±0,02 μg/kg (0,1 μg/kg)

±0,04 μg/kg (0,15 μg/kg)


0,5–5 µg/kg

CCα = 1,53 μg/kg

CCβ = 2,06 μg/kg

±0,57 μg/kg (0,5 μg/kg)

±0,65 μg/kg (1,0 μg/kg)

±0,98 μg/kg (1,5 μg/kg)


0,5–5 µg/kg

CCα = 1,40 μg/kg

CCβ = 1,79 μg/kg

±0,33 μg/kg (0,5 μg/kg)

±0,48 μg/kg (1,0 μg/kg)

±0,84 μg/kg (1,5 μg/kg)


0,25–2,5 µg/kg

CCα = 0,72 μg/kg

CCβ = 0,94 μg/kg

±0,13 μg/kg (0,25 μg/kg)

±0,27 μg/kg (0,5 μg/kg)

±0,46 μg/kg (0,75 μg/kg)


0,25–2,5 µg/kg

CCα = 0,61 µg/kg

CCβ = 0,73 µg/kg

±0,04 μg/kg (0,25 μg/kg)

±0,14 μg/kg (0,5 μg/kg)

±0,31 μg/kg (0,75 μg/kg)


0,5–5 µg/kg

CCα = 1,23 µg/kg

CCβ = 1,47 µg/kg

±0,24 μg/kg (0,5 μg/kg)

±0,28 μg/kg (1,0 μg/kg)

±0,33 μg/kg (1,5 μg/kg)


0,25–2,5 µg/kg

CCα = 0,65 µg/kg

CCβ = 0,79 µg/kg

±0,08 μg/kg (0,25 μg/kg)

±0,18 μg/kg (0,5 μg/kg)

±0,16 μg/kg (0,75 μg/kg)


0,1–1 µg/kg

CCα = 0,33 µg/kg

CCβ = 0,46 µg/kg

±0,07 μg/kg (0,1 μg/kg)

±0,16 μg/kg (0,2 μg/kg)

±0,17 μg/kg (0,3 μg/kg)


0,1–1 µg/kg

CCα = 0,27 µg/kg

CCβ = 0,34 µg/kg

±0,05 μg/kg (0,1 μg/kg)

±0,09 μg/kg (0,2 μg/kg)

±0,14 μg/kg (0,3 μg/kg)


0,25–2,5 µg/kg

CCα = 0,61 µg/kg

CCβ = 0,72 µg/kg

±0,10 μg/kg (0,25 μg /kg)

±0,13 μg/kg (0,5 μg /kg)

±0,14 μg/kg (0,75 μg /kg)





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No


0,25–2,5 µg/kg

CCα = 0,69 μg/kg

CCβ = 0,89 μg/kg

±0,09 μg/kg (0,25 μg/kg)

±0,24 μg/kg (0,5 μg/kg)

±0,15 μg/kg (0,75 μg/kg)


0,5–5 µg/kg

CCα = 1,07 µg/kg

CCβ = 1,14 µg/kg

±0,07 μg/kg (0,5 μg/kg)

±0,09 μg/kg (1,0 μg/kg)

±0,35 μg/kg (1,5 μg/kg)


0,5–5 µg/kg

CCα = 1,28µg/kg

CCβ = 1,56 µg/kg

±0,21 μg/kg (0,5 μg/kg)

±0,34 μg/kg (1,0 μg/kg)

±0,27 μg/kg (1,5 μg/kg)


0,5–5 µg/kg

CCα = 1,19 µg/kg

CCβ = 1,37 µg/kg

±0,14 μg/kg (0,5 μg/kg)

±0,23 μg/kg (1,0 μg/kg)

±0,23 μg/kg (1,5 μg/kg)


0,1–1 µg/kg

CCα = 0,31 µg/kg

CCβ = 0,42 µg/kg

±0,03 μg/kg (0,1 μg/kg)

±0,13 μg/kg (0,2 μg/kg)

±0,14 μg/kg (0,3 μg/kg)


0,5–5 µg/kg

CCα = 1,21 µg/kg

CCβ = 1,42 µg/kg

±0,25 μg/kg (0,5 μg/kg)

±0,26 μg/kg (1,0 μg/kg)

±0,80 μg/kg (1,5 μg/kg)



CCβ = 0,10 µg/kg


CCβ = 0,10 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,025 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,50 μg/kg


CCβ = 0,25 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,10 µg/kg


CCβ = 0,10 µg/kg


CCβ = 0,25 µg/kg


CCβ = 0,25 µg/kg


DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

CCβ = 0,50 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,50 µg/kg


CCβ = 0,10 µg/kg


CCβ = 0,50 µg/kg


Interna metoda /

In-house method

Določanje ergot alkaloidov v živilih in krmi;

LC-MS/MS / Determination of ergot

alkaloids; LC-MS/MS

Žita, krmne mešanice / Grains, compound feed

0,01–0,5 mg/kg

LOD = 0,003 mg/kg

LOQ = 0,01 mg/kg

Ergokornin / Ergocornine

0,007 mg/kg (0,01 mg/kg)

0,10 mg/kg (0,2 mg/kg)

0,25 mg/kg (0,5 mg/kg)

Ergokristin / Ergocristine

0,009 mg/kg (0,01 mg/kg)

0,14 mg/kg (0,2 mg/kg)

0,35 mg/kg (0,5 mg/kg)

Ergokriptin / Ergocryptine

0,006 mg/kg (0,01 mg/kg)

0,11 mg/kg (0,2 mg/kg)

0,28 mg/kg (0,5 mg/kg)

Ergometrin / Ergometrine

0,003 mg/kg (0,01 mg/kg)

0,14 mg/kg (0,2 mg/kg)

0,35 mg/kg (0,5 mg/kg)

Ergozin / Ergosine

0,005 mg/kg (0,01 mg/kg)

0,05 mg/kg (0,2 mg/kg)

0,13 mg/kg (0,5 mg/kg)

Ergotamin / Ergotamine

0,005 mg/kg (0,01 mg/kg)

0,05 mg/kg (0,2 mg/kg)

0,13 mg/kg (0,5 mg/kg)

Ergokorninin / Ergocorninine

0,007 mg/kg (0,01 mg/kg)

0,03 mg/kg (0,2 mg/kg)

0,08 mg/kg (0,5 mg/kg)

Ergokristinin / Ergocristinine

0,005 mg/kg (0,01 mg/kg)

0,05 mg/kg (0,2 mg/kg)

0,13 mg/kg (0,5 mg/kg)

Ergokriptinin / Ergocryptinine

0,007 mg/kg (0,01 mg/kg)

0,05 mg/kg (0,2 mg/kg)

0,13 mg/kg (0,5 mg/kg)

Ergometrinin / Ergometrinine

0,003 mg/kg (0,01 mg/kg)

0,03 mg/kg (0,2 mg/kg)

0,08 mg/kg (0,5 mg/kg)

Ergozinin / Ergosinine

0,003 mg/kg (0,01 mg/kg)

0,06 mg/kg (0,2 mg/kg)

0,15 mg/kg (0,5 mg/kg)

Ergotaminin / Ergotaminine

0,004 mg/kg (0,01 mg/kg)

0,05 mg/kg (0,2 mg/kg)

0,13 mg/kg (0,5 mg/kg)

DA / YES


152/2009, Priloga VI,

dopolnjena z Uredbo

Komisije (EU) št.

51/2013

Postopek za ugotavljanje prisotnosti tkiv

živalskega izvora; mikroskopska metoda

Analytical method for the determination of

costituents of animal origin; microscopic

method

Krma in sestavine krme / Feed and feed ingredients

Kvalitativna metoda / Qualitative method

DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No


(EC) No 152/2009,

Annex VI, amended by


(EU) No 51/2013

SOP 259, verzija 3


152/2009, Priloga IV,

Metoda F


(EC) No 152/2009,

Annex IV, Method F

Določanje diklazurila v krmi, premiksih in

koncentratih; HPLC-UV detekcija

Determination of diclazuril in feed, premixes

and concentrates; HPLC-UV detection

SOP 49, verzija 6

Krma / Feed

Premiksi / Premixes


0,5 mg/kg–5 g/kg

LOD = 0,2 mg/kg

LOQ = 0,5 mg/kg

0,15 mg/kg (0,5 mg/kg)


50 mg/kg (500 mg/kg)

0,03 % (5 g)

DA / YES

DA / YES

DA / YES



Metoda G


(EC) No 152/2009,

Annex IV, Method G

Določanje lasalocida v krmi, premiksih in

koncentratih; HPLC-fluorescenčna detekcija

Determination of lasalocid sodium in feed,

premixes and concentrates; HPLC-

fluorescence

SOP 117, verzija 6

Krma / Feed

Premiksi / Premixes


4,7 mg/kg–160 g/kg

LOD = 1,2 mg/kg

LOQ = 4,7 mg/kg

1 mg/kg (5 mg/kg)

5 mg/kg (100 mg/kg)

2,1 g/kg (20 g/kg)

1,2 % (160 g)

DA / YES

DA / YES

DA / YES



Metoda E


(EC) No 152/2009,

Annex IV, Method E

Določanje robenidina v krmi, premiksih in

koncentratih; HPLC-UV detekcija

Determination of robenidine in feed,

premixes and concentrates; HPLC-UV

detection

SOP 118, verzija 6

Krma / Feed

Premiksi / Premixes


3,2 mg/kg–66 g/kg

LOD = 1,2 mg/kg

LOQ = 3,2 mg/kg

1,2 mg/kg (3,2 mg/kg)

4,4 mg/kg (35,6 mg/kg)


1,3 g/kg (8 g/kg)

0,9 % (66 g)

DA / YES

DA / YES

DA / YES


Interna metoda /

In-house method

Določanje maduramicina v krmi, premiksih

in koncentratih; HPLC-UV detekcija

Determination of maduramicin ammonium

in feed, premixes and concentrates; HPLC-

UV detection

Krma / Feed

Premiksi / Premixes


1,0 mg/kg–10 g/kg

LOD = 0,4 mg/kg

LOQ = 1,0 mg/kg

0,3 mg/kg (1 mg/kg)

1,9 mg/kg (5 mg/kg)

0,3 g/kg (1 g/kg)

0,2 % (10 g)

DA / YES

DA / YES

DA / YES

97. SOP 197

Interna metoda /

In-house method

Določanje amprola; HPLC-UV detekcija

Determination of amprolium; HPLC-UV

detection

Krma / Feed


5 mg/kg–200 g/kg

LOD = 1,1 mg/kg

LOQ = 5 mg/kg

1,2 mg/kg (5,9 mg/kg)

2,1 mg/kg (29,4 mg/kg)

18 g/kg (200 g/kg)

NE / NO

98.

Uredba Komisije (ES) št.


Metoda C

Določanje surovih beljakovin

Determination of crude proteins

SOP 251, verzija 1

Krma / Feed

NE / NO



Metoda H

Določanje surovih maščob

Determination of crude fats

SOP 252, verzija 2

Krma / Feed NE / NO



Metoda I

Določanje surovih vlaknin

Determination of crude fibre

SOP 253, verzija 1

Krma / Feed

NE / NO



Metoda M

Določanje surovega pepela

Determination of crude ash

SOP 254, verzija 1

Krma / Feed

NE / NO





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No


152/2009, Priloga VI,

dopolnjena z Uredbo

Komisije (EU) št.

51/2013


(EC) No 152/2009,

Annex VI, amended by


(EU) No

51/2013

Postopek za ugotavljanje prisotnosti tkiv

živalskega izvora; PCR

Analytical method for the determination of

costituents of animal origin; PCR

Hrana za ribe / Feed and feed material for aquaculture


NE / NO

103. Metode IAG Mikroskopska preiskava krme / Microscopic

feed examination

SOP 380, verzija 1

SOP 396, verzija 1

Krma in sestavine krme / Feed and feed ingredients


NE / NO

104. SOP 257

Interna metoda /

In-house method

Določanje vitamina A in E; HPLC-UV

detekcija / Determination of vitamin A and

E; HPLC-UV detection

Krma / Feed NE / NO

Inštitut za mikrobiologijo in parazitologijo, Enota za bakteriologijo in mikologijo

Št.

No.



Preskusna metoda

Test method



Material, products; Measurement uncertainty (expressed as expanded measurement uncertainty U); Method limits

Akreditirano* Da/Ne

Accredited Yes/No

105. ISO 6579-1:2017



prisotnosti, števila in serotipov Salmonella

– 1. del: Ugotavljanje prisotnosti

Salmonella spp.


method for the detection, enumeration and

serotyping of Salmonella – Part 1:

Detection of Salmonella spp.

SOP 400, verzija 2

Krmila / Feedingstuffs

LOD = (1–5) CFU/25 g

Feces, vpojne prevleke za škornje, stelja, vzorci

živalskega okolja in površin / Faeces, organs, boot

swabs, litter, samples of animal environment and

surfaces

LOD = (5–10) CFU/25 g

Bakterijski izolati / Bacterial isolates

DA / YES

DA / YES

DA / YES

106. Kauffmann-White-Le

Minorjeva shema (WHO)

Kauffmann- White-Le

Minor scheme (WHO)

Serotipizacija sevov Salmonella enterica –

Ugotavljanje somatskih in flagelarnih

antigenov

Serotyping of Salmonella enterica -

Identification of somatic and flagellar

antigens

SOP 7, verzija 5


DA / YES


interna metoda

in-house method

Antibiogram – ugotavljanje občutljivosti

bakterij za antibiotike in kemoterapevtike

po mikrodilucijski metodi

Antimicrobial susceptibility testing of

bacteria – microdilution method

Interna metoda v skladu s priporočili EURL

za ugotavljanje odpornosti bakterij proti

protimikrobnim zdravilom

In-house method according to

recommendations of EURL for

antimicrobial resistance;

Temelji na standardu EUCAST

Based on EUCAST standard


merilna negotovost

± (0–2) log2 (glede na različne protimikrobne

učinkovine)

measurement uncertainty

± (0–2) log2 (regarding different antimicrobials)

DA / YES


interna metoda

in-house method

Preiskava na povzročitelje goveje

tuberkuloze (izolacija in identifikacija)

Examination for aetiologic agents of bovine

tuberculosis (isolation and identification)

Temelji na / based on:

OIE Manual of Diagnostic Tests and

Vaccines for Terrestrial Animals,

poglavje/chapter 3.3.6. (avian tuberculosis),

2014 in/and 3.4.6 (bovine tuberculosis),

2009

Klinični in patološki vzorci / Clinical and pathologic

samples

LOD = 72 CFU/g

Nastil, krma, voda /

litter, feeding-stuffs, water

DA / YES

NE / NO


Preiskava na bakterijo Listeria

monocytogenes in drugih listerij – SOP

Klinični in patološki vzorci, vzorci okolja / Clinical

and pathologic samples, environment samples

DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

interna metoda

in-house method

147: samo izolacija

Examination on Listeria monocytogenes

and Listeria spp. – SOP 147: detection only

LOD = (5–10) CFU/25 g

110. ISO 11290-1:2017

samo potrditev

confirmation only

Mikrobiologija v prehranski verigi –


prisotnosti in števila Listeria

monocytogenes in Listeria spp. – 1. del:

Metoda za ugotavljanje prisotnosti

Microbiology of the food chain –

Horizontal method for the detection and

enumeration of Listeria monocytogenes and

Listeria spp. – Part 1: Detection method

SOP 147, verzija 9

Bakterijski izolati / Bacterial isolates DA / YES

111. OIE Manual of

Diagnostic Tests and

Vaccines for Terrestrial

Animals,

poglavje/chapter 3.3.11.

(S. Gallinarum /

Pullorum), 2018 in/and

poglavje/chapter

3.9.8.(other Salmonellae),

2016

Preiskava kliničnega in patološkega

materiala živalskega izvora ter vzorcev

živalskega okolja na Salmonella spp. –

izolacija in potrditev

Examination of clinical and pathological

samples of animal origin (terrestrial

animals) and samples of animal

environment for Salmonella spp. – isolation

and confirmation

SOP 15 (izolacija/isolation), verzija 7

SOP 400 (potrditev/confirmation), verzija 2

Klinični in patološki vzorci živalskega izvora ter

vzorci živalskega okolja / Clinical and pathological

samples of animal origine (terrestrial animals) and

samples of aminal evironment

LOD = (1–5) CFU/25g

DA / YES

112. ISO 10272-1:2017

Postopek C

Procedure C

Izolacija in identifikacija termotolerantnih

Campylobacter spp.

Isolation and identification of thermophilic

Campylobacter spp.

SOP 187, verzija 4

Feces, organi, površine /

Faeces, organs, surfaces

LOD = 60 CFU/1 g

DA / YES

113. ISO 10272-1:2017

Potrditev in identifikacija

Confirmation and

identification

Potrditev in identifikacija termotolerantnih

Campylobacter spp.

Isolation and identification of thermophilic

Campylobacter spp.

SOP 187, verzija 4

Bakterijski izolati / Bacterial isolates DA / YES

114. OIE Manual of



Animals,

poglavje/chapter 3.1.4.,

2016

Rose bengal test za ugotavljanje protiteles

proti bakterijam Brucella abortus, Brucella

melitensis in Brucella suis

Rose bengal test for detection of antibodies

against Brucella abortus, Brucella

melitensis and Brucella suis

SOP 74, verzija 6

Serum domačih in divjih živali/ Serum of domestic and

wild animalls

DA / YES


interna metoda

in-house method

Dokaz tarčnih nukleinskih kislin

mikroorganizmov z metodo verižne

reakcije s polimerazo v realnem času (PCR

v realnem času)

Detection of target nucleic acids of

microorganisms using real-time polymerase

chain reaction (real-time PCR)

Real-time PCR method for the detection of

animal genome regions and animal

microorganisms genome regions

Molekularno dokazovanje bakterije Escherichia coli, ki

proizvaja Šigove toksine (STEC)

Molecular identification of STEC (Shiga toxin-

producing Escherichia coli)

SOP 461, verzija 5

Temelji na / based on: ISO 13136:2012

Molekularno dokazovanje bakterije

Francisella tularensis

Temelji na / based on: Fujita O, et al. (2006). Jpn J

Infect Dis, 59: 46-51.

Molekularno dokazovanje in determinacija izolatov

brucel

DA / YES

NE / NO

NE / NO

116. ISO 13136:2012 Molekularno dokazovanje bakterije

Escherichia coli, ki proizvaja Šigove

toksine (STEC)

Molecular identification of STEC (Shiga

toxin-producing Escherichia coli)

SOP 431, verzija 4

SOP 461, verzija 5

(faza obogatitve se izvaja na IVHKO, EVH

/ the enrichment step is performed at

IVHKO, EVH)

Živila

LOD = (0,95–10) CFU/25 g

DA / YES

117. interna metoda

in-house method

Ugotavljanje genov bakterije Escherichia

coli, ki proizvaja Šigove toksine (STEC) –

dokazovanje DNA bakterije z multipleks

PCR

Temelji na / based on: ISO 13136

Bakterijski izolati NE / NO





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

118. interna metoda

in-house method

Ugotavljanje genov za določanje

monofazne Salmonella Typhimurium –

dokazovanje DNA bakterije z multipleks

PCR

Temelji na / based on: Tennant SM, et al.

(2010). PloS Negl Trop Dis, 4: e621.


119. interna metoda

in-house method

protokol povzet po

navodilih komercialnega

kompleta / according to

the protocol of the

commercial kit Kylt SE

DIVA 1, AniCon Labor

Molekularna identifikacija vakcinalnega

seva 441/014 Salmonella Enteritidis –

dokazovanje DNA bakterije s PCR v

realnem času

Bakterijski izolati

NE / NO

120. interna metoda

in-house method

Genotipizacija bakterijskih izolatov –

tipizacija s sekvenciranjem celotnih

genomov (WGS)

Bakterijski izolati

NE / NO

121. OIE Manual of



Animals, chapter 3.1.4.

Test ELISA za ugotavljanje protiteles proti

brucelam v hlevskih vzorcih mleka govedi,

SOP 494

ELISA test for the detection of Brucella

antibodies in bovine tank milk samples

Mleko / Milk

NE / NO

122. SOP 103

interna metoda

in-house method

Antibiogram – ugotavljanje občutljivosti

bakterij za antibiotike in kemoterapevtike

po difuzijski metodi z diski

Antimicrobial susceptibilitity testing of

bacteria – disc diffusion method

Temelji na standardih CLSI in EUCAST

Based on CLSI and EUCAST standards


Ugotavljanje občutljivosti bakterij za protimikrobna

zdravila

Antimicrobial susceptibilitity testing of bacteria

NE / NO

123. ISO 16654

Preiskava na Escherichia coli O157

(izolacija in identifikacija)

Detection of Escherichia coli O157

(isolation and identification)

SOP 278

(priprava in inokulacija mesa se opravlja na

IVHKO, EVH / meat preparation and

inoculation at IVHKO, EVH)

Meso / Meat

LOD = (10–20) CFU/10 g

NE / NO

124. SOP 278

interna metoda

in-house method

Preiskava na Escherichia coli O157

(izolacija in identifikacija)

Detection of Escherichia coli O157

(isolation and identification)

Temelji na: ISO 16654 / Based on: ISO

16654

Feces, vsebina črevesa, bakterijski sevi / Faeces,

intestine content, bacterial strains

LOD = (10–20) CFU/10 g

NE / NO

125. interna metoda

in-house method

Molekularna identifikacija Bacillus

anthracis - dokazovanje DNA bakterije s

PCR v realnem času

Temelji na: OIE Manual of Diagnostic

Tests and Vaccines for Terrestrial Animals,

poglavje 3.1.1.

Bakterijski izolati, prah, tkivo, vzorci okolja NE / NO

126. OIE Manual of



Animals, poglavje 3.1.15

Postopek bakteriološke preiskave na

paratuberkulozo - izolacija M. avium subsp.

paratuberculosis

SOP 70

Organi, tkiva, iztrebki, krmila NE / NO

127. interna metoda

in-house method

Molekularna identifikacija Coxiella burnetii

- dokazovanje DNA bakterije s PCR v

realnem času

Mleko, kri, organi, feces, vzorci okolja NE / NO

128. OIE Manual of



Animals, poglavje 3.1.22.

Preiskava na tularemijo - izolacija in

determinacija Francisella tularensis

Kri in parenhimski organi zajcev, kuncev in drugih

občutljivih živali

NE / NO

129. OIE Manual of




Preiskava na povzročitelja hude gnilobe

čebelje zalege v medu - izolacija in

ugotavljanje števila Paenibacillus larvae

Med ali satje z medom, drobir, vosek NE / NO

130. SOP 459

interna metoda

in-house method

Preiskava na brucelozo – B. melitensis, B.

abortus, B. suis, B. ovis - izolacija in

preliminarna identifikacija do rodu

Organi, plod in plodove

ovojnice, seme goveda, prašičev,

ovc, koz in divjih živali

NE / NO

131. OIE Manual of




RVK bruceloza - metoda RVK za

ugotavljanje prisotnosti protiteles proti

bakterijam Brucella abortus, Brucella


SOP 477

Serum NE / NO





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

132. SOP 436

interna metoda

in-house method

Hitra seroaglutinacija (SA) za ugotavljanje

protiteles proti bakteriji Brucella canis

Serum NE / NO

133. OIE Manual of




Preiskava na govejo genitalno

kampilobakteriozo - izolacija in

identifikacija bakterije Campylobacter fetus

subsp. venerealis

Biki: seme, prepucialni izpirek ali bris;

Krave: vaginalna ali cervikovaginalna sluz, abortiran

fetus, placenta

NE / NO

134. OIE Manual of




Preiskava na Tritrichomonas foetus - dokaz

z gojiščno preiskavo

Seme in prepucialni izpirek bika NE / NO

135. OIE Manual of




Preiskava na infekciozni metritis kopitarjev

(metritis contagiosa equorum, CEM) -

izolacija in determinacija bakterije

Taylorella equigenitalis

Kobila: bris cerviksa, bris klitorisa, izcedek iz vagine ali

maternice, placenta;

Žrebec: bris uretre, fose uretralis, sluznice penisa ali

prepucija, preejakulatna tekočina

NE / NO

136. Metoda EURL Preiskava na MRSA - Izolacija MRSA v

kliničnem materialu in potrditev z

ugotavljanjem občutljivosti za antibiotike

Klinični brisi, organi in tkiva, vzorci okolja NE / NO

137. OIE Manual of




Postopek bakteriološke preiskave na

mikobakterije - izolacija mikobakterij

SOP 69

Organi, tkiva, iztrebki, krmila

NE / NO

138. interna metoda

in-house method

Genotipizacija bakterij Escherichia coli -

tipizacija izolatov s PFGE

Temelji na: Caprioli A, et al. (2014). EFSA

supporting publication: EN-704.


139. interna metoda

in-house method

Genotipizacija salmonel - tipizacija izolatov

s PFGE

Temelji na: Jacobs W et al. (2014). EFSA



140. interna metoda

in-house method

Genotipizacija Listeria monocytogenes –

tipizacija izolatov s PFGE

Temelji na: Roussel S, et al. (2014). EFSA



141. interna metoda

in-house method

Genotipizacija Campylobacter jejuni, C.

coli - tipizacija izolatov s PFGE

Temelji na: Ribot EM, et al. (2001). J Clin

Microbiol, 39: 1889-94.


142. interna metoda

in-house method

Preiskava na botulizem - izolacija

Clostridium botulinum in dokaz toksina

Temlji na: Quinn PJ, et al. Clinical

veterinary microbiology, Mosby, 2002:

196-200.

Organi in vsebina prebavil občutljivih živali, vzorci

okolja npr. blato, mulj; bakterijski izolati

NE / NO

143. interna metoda

in-house method

Preiskava na skupno število klic v semenu -

ugotavljanje števila živih bakterij v vzorcu

(predvsem aerobov in fakultativnih

ananerobov)

Metoda štetja na komercialnem gojišču

priznanem od AFNOR

Seme, prepucialni izpirki NE / NO

144. interna metoda

in-house method

Preiskava na dermatofite - mikroskopsko

dokazovanje dermatofitov v kužnini,

izolacija in determinacija dermatofitov

Temelji na: Larone DH. Medically

important fungi, A guide to identification.

Washington: American Society for

Microbiology, 1993.

Dlaka, ostružek in bris kože NE / NO

145. interna metoda

in-house method Molekularna identifikacija Renibacterium

salmoninarum – dokazovanje DNA

bakterije s PCR

Organi (ledvica, vranica, jetra), bakterijski izolati NE / NO

146. interna metoda

in-house method

Ugotavljanje genov Staphylococcus aureus

(MRSA) – dokazovanje DNA bakterije z

multipleks PCR


147. interna metoda

in-house method

Metoda RVK za ugotavljanje protiteles

proti protozoju Trypanosoma equiperdum

SOP 76

Serum NE / NO




Inštitut za mikrobiologijo in parazitologijo, Enota za virologijo

Št.

No

.

Oznaka preskusne metode Internal/standard

reference number

Preskusna metoda

Test method

Materiali, proizvodi; Merilna negotovost (podana kot

razširjena merilna negotovost U); Meje metode

Material, products; Measurement uncertainty(expressed as

expanded measurement uncertainty U); Method limits

Akreditirano* Da/Ne

Accredited Yes/No

148. OIE Manual of Diagnostic Tests

and Vaccines for Terrestiral

Animals chapter 3.4.7., 2015

Test ELISA za dokazovanje protiteles proti

virusu bovine virusne diareje v serumu goveda

ELISA test for detecting antibodies against the

bovine viral diarrhoea virus in bovine sera

SOP 246, verzija 6

Serum / Serum

Mleko / Milk

DA / YES

NE / NO



Animals, chapter 3.1.3., 2014

Protokol povzet po navodilih EURL

IAH Pirbright

according to the protocol of EURL

IAH Pirbright

Reverzna transkripcija in verižna reakcija s

polimerazo v realnem času (RT-qPCR) za dokaz

nukleinske kisline virusa bolezni modrikastega

jezika (BTV)

Reverse transcription and polymerase chain

reaction in real-time (RT-qPCR) for the

detection of nucleic acids of bluetongue virus

(BTV)

SOP 339, verzija 4

Polna kri / whole blood DA / YES



Animals chapter 3.8.3., 2019



nukleinske kisline virusa klasične prašičje kuge

(RT-qPCR KPK)

Reverse transcription and real-time polymerase

chain reaction to prove classical swine fever

virus nucleic acid (RT-qPCR CSFV)

SOP 370, verzija 6

Polna kri, serum, organi / whole blood,

serum, organs

DA / YES






nukleinske kisline virusa bovine virusne diareje

(BVD)



detection of nucleic acids of bovine viral

diarrhoea virus (BVDV)

SOP 465, verzija 3

Serum/ Serum

Organi /Organs

DA / YES

NE / NO




Dokaz protiteles proti virusu klasične prašičje

kuge z metodo ELISA –komercialni komplet

Detection of antibodies against classical

swine fever by ELISA – commercial kit

SOP 230, verzija 10

Serum / Serum

Serološka preiskava za določitev protiteles

proti virusu klasične prašičje kuge

/Serological determination of antibodies

against classical swine fever virus/

titer protiteles /antibody titer = 1: 10

DA / YES





virusu Infekcioznega bovinega rhinotraheitisa /

Infekcioznega bovinega pustulovulvovaginitisa v

serumu goveda


infectious bovine rhinotracheitis/infectious

bovine vulvovaginitis in bovine sera

SOP 271, verzija 8

Serum / Serum


proti virusu Infekcioznega bovinega

rhinotraheitisa / Infekcioznega bovinega

pustulovulvovaginitisa /Serological

determination of antibodies against virus

against the infectious bovine

rhinotracheitis/infectious bovine

vulvovaginitis virus/

Kvalitativna metoda/ Qualitative method

DA / YES




Dokaz protiteles proti virusu bolezni Aujeszkega

z metodo ELISA – komercialni komplet

Pseudorabies/Aujeszky disease virus

Detection of antibodies against Aujeszky disease

virus by ELISA – commercial kit


SOP 311, verzija 6

Serum / Serum

DA / YES




Test imunofluorescence (IF) za dokazovanje

virusa stekline

Fluorescent antibody test (FAT) for rabies virus

diagnosis

SOP 214, verzija 5

Odtis možganov/Brain smear

DA / YES





Št.

No.


reference number

Preskusna metoda

Test method



Material, products; Measurement uncertainty(expressed as expanded measurement uncertainty U); Method limits

Akreditirano* Da/Ne

Accredited Yes/No




Dokaz protiteles proti virusu bolezni

modrikastega jezika z metodo ELISA –

komercialni komplet

Detection of antibodies against Bluetongue virus

by ELISA – commercial kit

SOP 364, verzija 2

Serum / Serum

DA / YES




interna metoda

in-house method


polimerazo (RT-PCR) za dokaz nukleinske

kisline virusa bovine virusne diareje (BVDV)


reaction (RT-PCR) for the detection of nucleic

acids of bovine viral diarrhoea virus (BVDV)

SOP 277

Serum/ Serum

Organi /Organs

NE/ NO

NE/ NO




protokol povzet po navodilih

referenčnega laboratorija EU, IAH

Pirbright


IAH Pirbright

Test LPB ELISA za določanje protiteles proti

virusu slinavke in parkljevke tipov A, O, C

LPB ELISA test for the detection of antibodies

aginst foot and mouth disease against type A, O,

C

SOP 55

Serum / Serum

NE / NO






Pirbright


IAH Pirbright



nukleinske kisline virusa slinavke in parkljevke

(SIP), vseh 7 serotipov



detection of nucleic acids of foot and mouth

disease (FMD), all 7 serotypes

SOP 371

Vsebina afte, probang vzorec, epitelij kože /

Vesicula fluid, probang sample, epithelium,

NE / NO




Test ELISA – 3ABC za dokazovanje protiteles

proti NS proteinom virusa SIP

3ABC ELISA test for the detection of antibodies

aginst NS protein of FMD virus

SOP 500

Serum / Serum

NE / NO




Test ELISA za določanje protiteles proti virusu

vezikularne bolezni prašičev (VBP)

ELISA test for the detection of swine vesicular

disease antibodies (SVD)

SOP 319

Serum / Serum

NE / NO






Pirbright


IAH Pirbright



nukleinske kisline virusa vezikularne bolezni

prašičev (VBP)



detection of nucleic acids of swine vesicular

disease (SVD)

SOP 404

Vsebina afte, probang vzorec, epitelij kože /

Vesicula fluid, probang sample, epithelium,

NE / NO




Test ELISA za določanje protiteles proti virusu

afriške prašičje kuge (APK)

ELISA test for the detection of african swine

fever antibodies (ASF)

SOP 308, verzija 4

Serum / Serum

DA / YES




Test ELISA za določanje virusa afriške prašičje

kuge (APK) v prašičjem serumu

ELISA test for the detection of african swine

fever virus (ASF)

SOP 423

Serum / Serum

NE / NO





Št.

No.


reference number

Preskusna metoda

Test method




Akreditirano* Da/Ne

Accredited Yes/No





referenčnega laboratorija EU,

CISA-INIA Valdeolmos



Verižna reakcija s polimerazo v realnem času

(qPCR) za dokaz nukleinske kisline virusa

afriške prašičje kuge (APK)

Polymerase chain reaction in real-time (qPCR)

for the detection of nucleic acids of african

swine fever virus (ASF)

SOP 405

Polna kri, serum, organi / Whole blood,

serum, organs

NE / NO





referenčnega laboratorija EU,




Verižna reakcija s polimerazo času (PCR) za

dokaz nukleinske kisline virusa afriške prašičje

kuge (APK)

Polymerase chain reaction (PCR) for the

detection of nucleic acids of african swine fever

virus (ASF)

SOP 340

Polna kri, serum, organi / Whole blood,

serum, organs

NE / NO




Dokazovanje protiteles proti virusu afriške

prašičje kuge (APK) z metodo imunobloting (IB)

Detection of african swine fever virus

abntibodies (ASF) using imunobloting methods

SOP 403

Serum / Serum

NE / NO




Postopek testa ELISA za določanje specifičnih

serumskih protiteles proti virusu bolezni

modrikastega jezika - Blutoungue virus VP7

antibody kit

Detection of antibodies against Bluetongue virus

by ELISA – commercial kit Blutoungue virus

VP7 antibody kit

SOP 365

Serum / Serum

NE / NO





virusu stekline (Biopro Rabies ELISA Ab test)

ELISA test for the detection of rabies antibodies

(Biopro Rabies ELISA Ab test)

SOP 449

Serum / Serum

NE / NO




Postopek za dokazovanje protiteles proti virusu

stekline z FAVN testom

Flourescent antibody virus neutralisation test

(FAVN) for the detection of antibodies against

rabies

SOP 321

Serum / Serum

NE / NO




Izolacija virusa stekline na celični kulturi

Isolation of rabies virus on cell culture

SOP 402

Vzorec možgan/ Brain sample NE / NO






kisline virusa stekline



acids of rabies virus

SOP 355

Vzorec možgan/ Brain sample NE / NO




Določanje titra stekline virusa SAD B19 v

cepivu Fucsoral®

Determination of titre of rabies virus SAD B19

in vaccine Fucsoral®

SOP 426

Cepivo / Vaccine NE / NO




Izolacija in virusa IBR/IPV na celični kulturi

Isolation of IBR/IPV on cell culture

SOP 216

Organi, seme / organs, semen NE / NO





Št.

No.


reference number

Preskusna metoda

Test method




Akreditirano* Da/Ne

Accredited Yes/No



Animals, chapter 3.4.11


virusu Infekcioznega bovinega rhinotraheitisa /

Infekcioznega bovinega pustulovulvovaginitisa v

serumu goveda


infectious bovine rhinotracheitis/infectious

bovine vulvovaginitis in bovine sera

SOP 361

Serum / Serum


proti virusu Infekcioznega bovinega


pustulovulvovaginitisa /Serological

determination of antibodies against virus

against the infectious bovine

rhinotracheitis/infectious bovine

vulvovaginitis virus/

Kvalitativna metoda/ Qualitative method

NE / NO




EU Commission decision

(2002/106/EC), chapter VI C in EU

Diagnostic Manual for Classical

swine fever (CSF) diagnosis

Virus nevtralizacijski test (VNT) za dokaz

protiteles proti virusu klasične prašičje kuge

Virus neutralisation test (VNT) for the detection

of antibodies against classical swine fever

SOP 061

Serum / Serum

NE / NO









protiteles proti virusu bovine virusne diareje


of antibodies against bovine viral diarrhoea virus

SOP 306

Serum / Serum

NE / NO









protiteles proti virusu borderske bolezni ovac

(BB)


of antibodies against border disease virus (BDV)

SOP 307

Serum / Serum

NE / NO




Virus nevtralizacijski test (VNT) za dokazovanje

protiteles proti virusu Infekcioznega bovinega


pustulovulvovaginitisa v serumu goveda Virus

neutralisation test (VNT) for detecting

antibodies against the infectious bovine

rhinotracheitis/infectious bovine vulvovaginitis

in bovine sera

SOP 419

Serum / Serum

NE / NO

180. 1

1

OIE Manual of Diagnostic Tests







Izolacija virusa klasične prašičje kuge (KPK) na

celični kulturi

Isolation of classical swine fever (CSF) virus on

cell culture

SOP 060


serum, organs

NE / NO




Izolacija virusa BVD na celični kulturi

Isolation of bovine viral diarrhoea virus on cell

culture

SOP 320


serum, organs

NE / NO










kisline pestivirusov in virusa klasične prašičje

kuge (KPK)



acids of pestiviruses and classical swine fever

virus (CSF)

SOP 293

Serum, organi / Serum, organs

NE / NO




Izolacija virusa kužnega arteritisa kopitarjev na

celični kulturi

Isolation of equine viral arteritis (EAV) on cell

culture

SOP 215

Laringotrahealni birs, abortirani fetus,

sperma / Laringotracheal swabs, aborted

foetuses, semen

NE / NO





Št.

No.


reference number

Preskusna metoda

Test method




Akreditirano* Da/Ne

Accredited Yes/No





protiteles proti virusu kužnega arteritisa

kopitarjev


of antibodies against equine viral arteritis (EAV)

SOP 362

Serum / Serum

NE / NO




Izolacija in virusa bolezni Aujeskega na celični

kulturi

Isolation of Aujeszky disease virus on cell

culture

SOP 359

Organi / Organs

NE / NO





protiteles proti virusu bolezni Aujeszkega (BA)


of antibodies against Aujeszky disease virus

(AD)

SOP 318

Serum / Serum

NE / NO




Dokaz protiteles proti virusu bolezni Aujeszkega

z metodo ELISA – komercialni komplet

Pseudorabies/Aujeszky disease virus kit PRV-

gE-Ab (Svanova)



Pseudorabies/Aujeszky disease virus kit PRV-

gE-Ab (Svanova)

SOP 452

Serum / Serum

NE / NO

188. OIE Manual of Diagnostic Tests for

Aquatic Animals, chapters 2.3.4.,

2.3.10.

Izolacija in virusov VHS, IHN, in IPN na celični

kulturi

Isolation of VHS, IHN, in IPN on cell culture

SOP 360

Organi, ovarialna tekočina / Organs, ovarial

fluids

NE / NO


Aquatic Animals, chapter 2.3.10.



kisline virusne hemoragične septikemije (VHS)



acids of viral haemorrhagic septicemia virus

(VHS)

SOP 352


fluids

NE / NO


Aquatic Animals, chapter 2.3.4..



kisline infekciozne hematopoetske nekroze

(IHN)



acids of infectious haematopoietic necrosis virus

(IHN)

SOP 353


fluids

NE / NO

191. SOP 354

Interna metoda

in-house method



kisline nalezljive nekroze trebušne slinavke

(IPN)



acids of infectious pancreatic necrosis virus

(IPN)


fluids

NE / NO






kisline virusa prašičjega reprodukcijskega in

respiratornega sindroma (PRRS)



acids of porcine reproductive and respiratory

syndrome virus (PRRS)

SOP 418

Organi, serum / Organs, serum

NE / NO


Aquatic Animals, chapter 2.3.7.

Verižna reakcija s polimerazo (PCR) za dokaz

nukleinske kisline KOI herpes virusa (KHV)

Polymerase chain reaction (PCR) for the

detection of nucleic acids of KOI herpes virus

(KHV)

SOP 429

Organi / Organs

NE / NO





Št.

No.


reference number

Preskusna metoda

Test method




Akreditirano* Da/Ne

Accredited Yes/No

194. SOP 406

Interna metoda /

in-house method



kisline čebeljih virusov: ABPV, BQCV, CBPV,

DWV, KBV, SBV



acids of bee viruses: ABPV, BQCV, CBPV,

DWV, KBV, SBV

Čebele mrtvice / dead bees NE / NO

195. SOP 457

Interna metoda /

in-house method


virusu Schmallenberg (ID Screen Schmallenberg

virus indirect Multi species– komercialni

komplet)

ELISA test for the detection of Schmallenberg

virus antibodies (ID Screen Schmallenberg virus

indirect Multi species– commercial kit)

Serum / Serum

NE / NO


and Vaccines for Terrestrial


Interna metoda /

in-house method

Verižna reakcija s polimerazo v realnem času

(qPCR) za dokaz nukleinske kisline virusa

vozličastega dermatitisa (LSD)

Polymerase chain reaction in real-time (qPCR)

for the detection of nucleic acids of Lumpy skin

disease virus (LSD)

SOP 487

Polna kri, brisi, organi /

Whole blood, swabs, organs

NE / NO




ELISA za ugot. protiteles proti virusu

infekciozne anemije kopitarjev (IAK) -

ugotavljanje specifičnih protiteles proti virusu

SOP 229

Serum NE / NO




AGID test za ugot. protiteles proti virusu

infekciozne anemije kopitarjev (IAK) -

ugotavljanje specifičnih protiteles proti virusu

SOP 433

Serum NE / NO




Verižna reakcija s polimerazo v realnem času za

dokaz nukleinske kisline virusa afriške prašičje

kuge (qPCR APK), virotype® ASFV PCR

Real time polymerase chain reaction for the

detection of nucleic acids of african swine fever

virus (qPCR ASF), virotype® ASFV PCR

SOP 490, verzija 1

Polna kri, serum, organi, bris, suspenzija

celične kulture / Whole blood, serum, organs,

swabs, cell culture suspension

DA / YES





virusu slinavke in parkljevke, tip A v serumu

parklarjev

(IZSLER FMD – Ab, type A)


Foot and mouth disease, serotype A

(IZSLER FMD-Ab, type A)

SOP 491

Serum / Serum

NE / NO

201. SOP 493

Interna metoda/

In-house method


virusu hepatitisa E

(PrioCHECK® HEV Ab porcine-komercialni

komplet)

ELISA for detection of hepatitis E antibodies

(PrioCHECK® HEV Ab porcine-commercial

kit)

Serum/Serum NE/NO

Inštitut za mikrobiologijo in parazitologijo, Enota za parazitologijo

Št.

No

.


reference number

Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

202. Uredba komisije (ES) št. 2015/1375

Priloga I,

Poglavje I, referenčna metoda

odkrivanja (digestivna m.)

Commission Regulation (EC) no

2015/1375; Annex I;

Chapter I, reference method of

detection, magnetic stirrer method

for pooled sample digestion

(digestive m.)

Preiskava na Trichinella spp. z digestivno

metodo

Examination for Trichinella spp. by digestive

method

SOP 8, verzija 9

Meso / Meat DA / YES




Inštitut za mikrobiologijo in parazitologijo, Enota za parazitologijo

Št.

No

.


reference number

Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No




Preiskava na ehinokokozo - ugotavljanje

ehinokoka na notranjih organih

SOP 470

Notranji organi NE / NO




Preiskava na babeziozo - dokaz v krvnem

razmazu obarvanem po Giemsi

Kri živali z antikoagulansom NE / NO




Preiskava na cisticerkozo - dokazovanje

prisotnosti cisticerkov v mišičnini

SOP 488

Mišičnina NE / NO




Preiskava na garjavce - dokaz z mikroskopsko

preiskavo kožnega ostružka po obdelavi s KOH

Kožni ostružek različnih vrst živali NE / NO




Inštitut za patologijo, divjad, ribe in čebele; Enota za patologijo ter sodno in upravno veterinarstvo

Št.

No.


reference number

Preskusna metoda

Test method

Materiali, proizvodi; Merilna negotovost (podana

kot razširjena merilna negotovost U); Meje

metode

Material, products; Measurement

uncertainty(expressed as expanded measurement

uncertainty U); Method limits

Akreditirano* Da/Ne

Accredited Yes/No

207. Uredba (EC) št. 999/2001, Aneks III,

Aneks X

Regulation (EC) No. 999/2001,

Annex III, Annex X

Postopek za diagnostiko bovine spongiformne

encefalopatije (BSE) pri govedu in TSE pri

drobnici s testom Prionics - Check WESTERN

Procedure for the diagnostics of bovine

spongiform encephalopathy (BSE )in cattle and

TSE in sheep and goats with Prionics - Check

WESTERN test

SOP 168, verzija 11

Možgani goveda in drobnice /

Bovine and small ruminant brain

kvalitativna metoda/qualitative

method

DA / YES


Aneks X


Annex III, Annex X

OIE Manual of diagnostic tests and

vaccines for terrestrial animals,



drobnici s patohistološko preiskavo možganov

Procedure for the diagnostic of bovine spongiform

encephalopathy (BSE) in cattle and TSE in sheep

and goats with histopathology of the brain

SOP 167, verzija 7




method

DA / YES


Aneks X


Annex III, Annex X

OIE Manual of diagnostic tests and

vaccines for terrestrial animals,

Postopek za ugotavljanje prionov v možganih

goveda in drobnice z imunohistokemično metodo

Procedure for detecting prions in bovine and

small ruminant brains by immunohistochemistry

SOP 234, verzija 7




method

DA / YES


Aneks X


Annex III, chapter A

Annex X, Chapter C



drobnici s testom Idexx HerdChek BSE-Scrapie

Antigen (IDEXX Laboratories)

Procedure for the diagnostics of bovine

spongiform encephalopathy (BSE) in cattle and

TSE in sheep and goats with the test Idexx

HerdChek BSE-Scrapie Antigen (IDEXX

Laboratories)

SOP 177, verzija 6




method

DA / YES

Inštitut za predklinične vede; Enota za biokemijo, molekularno biologijo in genetiko

Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No


interna metoda

in-house method

Genotipizacija gena PRNP pri ovcah s testom

ločevanja alelov z metodo verižne reakcije s

polimerazo v realnem času

Sheep PRNP gene genotyping with allelic

discrimination assay using real time polymerase

chain reaction

Aleli 136, 141, 154 in 171 gena PRNP pri

ovcah

Alleles 136, 141, 154 and 171 of PRNP gene in

sheep

DA / YES


interna metoda

in-house method

Določevanje speciesa v mesu in procesirani hrani

z metodo verižne reakcije s polimerazo v realnem

času

Species determination in meat and processed food

by real time polymerase chain reaction

Določanje mitohondrijske DNA, specifične za

konja (Equus cabalus);

>1 % celokupne DNA

Determination of mitochondrial DNA specific

for horse (Equus cabalus);

> 1 % total DNA

DA / YES




Inštitut za perutnino, ptice, male sesalce in plazilce

Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

213. Council Directive

92/66/EEC, Annex 3

OIE Manual of



Animals, Chapter 3.3.14,

2012

Postopek za test inhibicije hemaglutinacije za

določitev protiteles proti virusu atipične kokošje

kuge (APMV - 1)

Procedure for haemagglutination inhibition test

for detection of antibodies against Newcastle

disease virus (APMV – 1)

SOP 115, verzija 4

Serum perutnine / Poultry serum

DA / YES

214. Council Directive

2005/94/ES

Manual of diagnostic for

Avian Influenza

(Commission decision

2006/437/ES)

OIE Manual of




2015

Postopek za test inhibicije hemaglutinacije za

določitev protiteles proti virusu aviarne influence

podtip H5 in H7

Procedure for haemagglutination inhibition test

for detection of antibodies against Avian Influenza

subtype H5 and H7

SOP 247, verzija 2


DA / YES

215. OIE Manual of



Animals,

Chapter 3.1.12, 2014

modificirana metoda

modified method

Postopek za serološko preiskavo (test

mikroskopske aglutinacije) na prisotnost protiteles

proti leptospirozi

Procedure for serological examination

(microscopic agglutination test) for leptospiral

antibodies

SOP 120, verzija 4

Serum domačih živali / Serum of domestic

animals

DA / YES

216. OIE; Manual of




2015

Postopek za izvajanje testa hitre serumske

aglutinacije za dokaz protiteles proti Mycoplasma

gallisepticum

Procedure for rapid serum agglutination test for

detection antibodies against Mycoplasma

gallisepticum

SOP 275, verzija 3


NE / NO

217. OIE Manual of




2015

Ugotavljanje protiteles proti bakteriji Mycoplasma

gallisepticum (MG) z metodo ELISA

Procedure for rapid serum agglutination test for

detection of antibodies against Mycoplasma

gallisepticum (MG) by ELISA

SOP 483, verzija 1


DA / YES


Interna metoda /

In-house method

Dokaz tarčnih nukleinskih kislin

mikroorganizmov z metodo verižne reakcije s

polimerazo v realnem času (PCR v realnem času)

Detection of target nucleic acids of

microorganisms using real-time polymerase chain

reaction (real-time PCR)

Real-time PCR method for the detection of animal

genome regions and animal microorganisms

genome regions

Odsek gena za protein matrix virusov influence

tipa A, SOP 413, ver.4

Region of matrix protein gene of influenza type

A viruses, SOP 413

Odsek gena za protein hemaglutinin virusov

influence podtipa H7, SOP 414, ver.1

Region of hemagglutinin protein gene of H7

subtype influenza viruses, SOP 414

Odsek gena za protein hemaglutinin virusov

influence podtipa H5, SOP 415, ver.1

Region of hemagglutinin protein gene of H5


Odsek gena za protein neuraminidazo virusov

influence podtipa N1, SOP 416, ver.1

Region of neuraminidase protein gene of N1


DA / YES

NE / NO

NE / NO

NE / NO




Inštitut za perutnino, ptice, male sesalce in plazilce

Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

219. OIE Manual of




2015, Komisija evropskih

skupnosti, 2006. Odločba

komisije z dne 4. avgust

2006 o odobritvi

diagnostičnega priročnika

za aviarno influenco v

skladu z Direktivo Sveta

2005/94/ES,

2006/437/ES. UL L 10,

14.1.2006, str. 16

Določanje gena za protein M virusov influence

tipa A z metodo real-time Taqman RT-PCR

Detection of protein M gene of type A influenza

viruses by real-time Taqman RT-PCR

SOP 431, verzija 4

SOP 413, verzija 4

Izolirana RNA / Isolated RNA DA / YES

220. OIE Manual of



Animals, Chapter 3.3.4.,




2006 o odobritvi




2005/94/ES,

2006/437/ES. UL L 10,

14.1.2006, str. 16.

Določanje evrazijskih virusov aviarne influence

podtipa H7 z metodo real-time Taqman RT-PCR

Detection of Eurasian H7avian influenza viruses

by real-time Taqman RT-PCR

SOP 431, verzija 4

SOP 414 , ver.1

Izolirana RNA / Isolated RNA NE / NO

221. OIE Manual of



Animals,

Chapter 3.3.4., 2015,

Komisija evropskih



2006 o odobritvi




2005/94/ES,

2006/437/ES. UL L 10,

14.1.2006, str. 16.

Določanje evrazijskih virusov aviarne influence

podtipa H5 z metodo real-time Taqman RT-PCR

Detection of Eurasian H5 avian influenza viruses

by real-time Taqman RT-PCR

SOP 431, verzija 4

SOP 415, ver.1


222. OIE Manual of



Animals, Chapter 3.3.4.,




2006 o odobritvi




2005/94/ES,

2006/437/ES. UL L 10,

14.1.2006, str. 16.

Določanje gena za protein N1 virusov aviarne

influence z metodo real-time Taqman RT-PCR

Detection of protein N1 gene of avian influenza

viruses by real-time Taqman RT-PCR

SOP 431, verzija 4

SOP 416, ver.1





Klinika za reprodukcijo in velike živali, Klinika za prežvekovalce in prašiče

Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

223. OIE Manual of



Animals,

poglavje/chapter 3.8.6.

ELISA za dokaz protiteles proti prašičjemu

reprodukcijskemu in respiratornemu sindromu

(PRRS)

ELISA for detection of antibodies against porcine

reproductive and respiratory syndrome (PRRS)

SOP 89

Serum / Serum NE / NO

224. OIE Manual of



Animals,

poglavje/chapter 3.9.8

ELISA za dokaz protiteles proti salmonelam

(serološke skupine O:4 (B), O:7 (C1), O:9 (D1)),

pri prašičih

ELISA test for detecting of antibodies against

Salmonella (serogroups O:4 (B), O:7 (C1), O:9

(D1)) in swine

SOP 358

Serum, mesni sok

Serum, meat juice

NE / NO

Nacionalni veterinarski inštitut, Enota Maribor - Ptuj

Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

225. ISO 6579-1:2017 Mikrobiologija v prehranski verigi -Horizontalna

metoda za ugotavljanje prisotnosti, števila in

serotipov Salmonella – 1. del: Ugotavljanje

prisotnosti Salmonella spp.

Microbiology of the food chain - Horizontal method

for the detection and enumeration of Salmonella spp.

SOP 400, verzija 2

Meso in mesni izdelki / Meat and meat

products

LOD = 5–10 CFU/25 g

Mleko in mlečni izdelki / Milk and milk

products

LOD = 5-10 CFU/25 g (ml)

Jajca in jajčni izdelki / Eggs and egg

products

LOD = 5–10 CFU/vzorec

Feces in vzorci okolja / Faeces and

environmental samples

(oz. vzorec vpojnih prevlek in brisov

površin / or boot swab and surface sample)

LOD = 2–3 CFU/25 g

Kvalitativna metoda/qualitative method

DA / YES

DA / YES

DA / YES

DA / YES


Interna metoda

In-house method

Postopek molekularnega ugotavljanje Salmonel v

živilih, krmi, in vzorcih okolja s sistemom MDS

Salmonella

Molecullar detection system for detection of

Salmonella in food, feed and food process

environmental samples with MDS Salmonella

Vzorci prehranske verige

LOD = 3-5 CFU/25 g

Vzorci primarne proizvodnje


DA / YES

NE / NO

227. ISO 11290-1:2017

Mikrobiologija v prehranski verigi - Horizontalna

metoda za ugotavljanje prisotnosti in števila Listeria

monocytogenes in Listeria spp.- 1. del: Ugotavljanje

prisotnosti


for the detection and enumeration of Listeria

monocytogenes and Listeria spp.-


SOP 162, verzija 5


products

LOD = 5–10 CFU/25 g


products

LOD = 5–10 CFU/25 g (ml)

Brisi okolja / environmental swabs

LOD = 7 CFU/površina

DA / YES

DA / YES

DA / YES

228. ISO 11290-2: 2017


metoda za ugotavljanje prisotnosti in števila Listeria

monocytogenes in Listeria spp.- 2.del: Ugotavljanje

števila


for detection and enumeration of Listeria

monocytogenes and of Listeria spp. - Part 2:

Enumeration method

SOP 314, verzija 3


products

LOD = 10 CFU/g

U= ± 0,18 log10CFU/g


products

LOD = 10 CFU/g (ml)

U= ± 0,24 log10CFU/g (tekočine/liquids)

U= ± 0,24 log10CFU/g (trdni vzorci/solid milk products)

DA / YES

DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

229. ISO 6888-1:1999


Enumeration of coagulase-positive staphylococci (S.

aureus and other species) by Baird-Parker agar

medium

SOP 203, verzija 3


products

LOD = 10 CFU/g (ml)




products

LOD = 10 CFU/g


DA / YES

DA / YES

230. ISO 4833-1: 2013


(tehnika štetja kolonij na ploščah inkubiranih pri 30 oC)

Microbiology of the food chain – Horizontal method

for the enumeration of microorganisms Part 1:

Colony count at 30 oC by the pour plate technique

SOP 206, verzija 4


products

LOD = 10 CFU/g



products

LOD = 10 CFU/g (ml)



DA / YES

DA / YES

231. ISO 16649-2 : 2001

Določanje števila β glukoronidaza pozitivnih bakterij

Escherichia coli


Microbiology of food and animal feeding stuffs -

Horizontal method for the enumeration of β


Part 2:Colony count technique at 44o C

SOP 315, verzija 2

Meso in mesni izdelki /

Meat and meat products

LOD = 10 CFU/g


Mleko in mlečni izdelki /

Milk and milk products

LOD = 10 CFU/g (ml)

U= ± 0,14 log10 CFU/g (tekočine/liquids)

U= ± 0,2 log10 CFU/g (trdne snovi/solid milk products)

DA / YES

DA / YES

232. ISO 21528-2:2017 Mikrobiologija v prehranski verigi - Horizontalna

metoda za ugotavljanje prisotnost in števila

enterobakterij - 2.del: Metoda štetja kolonij


for the detection and enumeration of

Enterobacteriaceae - Part 2: Colony count technique

SOP 317, verzija 3



LOD = 10 CFU/g




LOD = 10 CFU/g (ml)

U= ± 0,21 log10 CFU/g

Brisi in izsečki trupov / Environmental

swabs

LOD = 10 CFU/cm2

U= ± 0,18 log10 CFU/cm2

Jajca in jajčni izdelki / Eggs and eggs

products

LOD = 10 CFU/g (ml)

DA / YES

DA / YES

DA/YES

NE / NO

233. ISO 10272-1: 2017

Postopek A in C

Procedure A and C


metoda za ugotavljanje prisotnosti in števila

Campylobacter spp. -1.del: Metoda za ugotavljanje

prisotnosti


for detection and enumeration of Campylobacter spp.


SOP 187, verzija 4



LOD = 2–3 CFU/g

Feces, površine / Faeces, surfaces

LOD = 60 CFU/g

DA / YES

DA / YES

234. OIE Manual of Diagnostic

Tests and Vaccines for

Terrestiral Animals,

chapter 3.8.3., 2019

Dokaz protiteles proti virusu klasične prašičje kuge z

metodo ELISA –komercialni komplet



SOP 230, verzija 10

Serum / Serum


proti virusu klasične prašičje kuge

/Serological determination of antibodies

against classical swine fever virus/

titer protiteles /antibody titer = 1: 10

DA / YES



Terrestiral Animals,

chapter 3.1.2, 2018

Dokaz protiteles proti virusu bolezni Aujeszkega z

metodo ELISA – komercialni komplet





SOP 311, verzija 6

Serum / Serum

DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No


Interna metoda

In-house method

Ugotavljanje zaviralnih substanc v mleku

(DELVOTEST®SP-NT)

Detection of the presence of inhibitory substances

in milk (DELVOTEST®SP-NT)

mleko / milk


CCβ = 3 µg/kg


CCβ = 4 µg/kg


CCβ = 4 µg/kg


CCβ = 20 µg/kg


not sensitive


not sensitive


not sensitive


CCβ = 20 µg/kg


CCβ = 50 µg/kg


CCβ = 50 µg/kg


CCβ = 10 µg/kg


CCβ = 70 µg/kg






not sensitive


not sensitive

neomicin / neomycin

CCβ = 500 µg/kg






not sensitive


not sensitive


not sensitive


CCβ = 90 µg/kg















tilozin / tylosin

CCβ = 50 µg/kg



DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

237. Uredba komisije (ES) št.

2015/1375 Priloga I,

Poglavje I, referenčna

metoda odkrivanja

(digestivna m.)


(EC) no 2015/1375; Annex

I;

Chapter I, reference

method of detection,

magnetic stirrer method

for pooled sample

digestion (digestive m.)

Preiskava na Trichinella spp. z digestivno metodo


method

SOP 8, verzija 9


238.

ISO 10272-2 : 2017



Campylobacter spp.- 2.del: Tehnika štetja kolonij


for detection and enumeration of Campylobacter spp.


SOP 385 verzija 3

Meso in mesni izdelki


LOD = 10 CFU/g

± 0,23 log10 CFU/g

DA / YES

239. OIE Manual of Standards

for Diagnostic Tests and



Preiskava na povzročitelja hude gnilobe čebelje

zalege v medu - izolacija in ugotavljanje števila

Paenibacillus larvae


240. Larone D.H., Medically

important Fungi, A guide

to identification.

Washington: American

Society for Microbiology


dokazovanje dermatofitov v kužnini, izolacija in

determinacija dermatofitov


241. SOP 147: 1. del

Interna metoda

SOP147: part 1

In-house method

Preiskava na bakterijo Listeria monocytogenes -

izolacija

Isolation of Listeria monocytogenes

SOP 147: 1. del/part 1

potrditev/confirmation: SOP 147, 2. del/part 2

Klinični in patološki vzorci / Clinical and

pathologic samples

LOD = 5–10 CFU/25 g

NE / NO




Nacionalni veterinarski inštitut, Enota Novo mesto

Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No


Interna metoda/in-house

method


(DELVOTEST®SP-NT)

Detection of the presence of inhibitory

substances in milk (DELVOTEST®SP-NT)

mleko / milk


CCβ = 3 µg/kg


CCβ = 4 µg/kg


CCβ = 4 µg/kg


CCβ = 20 µg/kg


not sensitive


not sensitive


not sensitive


CCβ = 20 µg/kg


CCβ = 50 µg/kg


CCβ = 50 µg/kg


CCβ = 10 µg/kg


CCβ = 70 µg/kg






not sensitive


not sensitive

neomicin / neomycin

CCβ = 500 µg/kg






not sensitive


not sensitive


not sensitive


CCβ = 90 µg/kg















tilozin / tylosin

CCβ = 50 µg/kg



DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

243.

SOP 391

interna metoda

in-house method

Preiskava na tetraciklinske in beta-laktamske

antibiotike v mleku s testom TWINSENSOR

Screening method for tetracyclines and beta

lactams antibiotics in milk - TWINSENSOR

mleko / milk


CCβ = 4 µg/kg


CCβ = 4 µg/kg

amoksicilin / amoksicillin

CCβ = 4 µg/kg


CCβ = 20 µg/kg


CCβ = 60 µg/kg


CCβ = 60 µg/kg


CCβ = 20 µg/kg


CCβ = 20 µg/kg


CCβ = 50 µg/kg


CCβ = 50 µg/kg


CCβ = 50 µg/kg


CCβ = 100 µg/kg


CCβ = 100 µg/kg

oksitetraciklin / oxytetracycline (OTC) CCβ =

100

µg/kg


CCβ = 30 µg/kg

dihidrostreptomicin / dihydrostreptomicine

not sensitive


not sensitive


not sensitive

neomicin / neomycin

not sensitive

streptomicin / streptomycine



not sensitive


not sensitive


not sensitive


not sensitive

sulfadimidin / sulfadimidine

not sensitive


not sensitive


not sensitive


not sensitive


not sensitive

tilozin / tylosin

not sensitive

trimetoprim / trimitroprim

NE / NO



Poglavje I, referenčna metoda

odkrivanja (digestivna m.)

Commission Regulation (EC)

no 2015/1375; Annex I;

Chapter I, reference method of

detection, magnetic stirrer

method for pooled sample


Preiskava na Trichinella spp. z digestivno

metodo


method

SOP 8, verzija 9




Terrestrial Animals,

poglavje/chapter 3.1.4, 2016

Rose bengal test za ugotavljanje protiteles

proti bakterijam Brucella abortus, Brucella



against Brucella abortus, Brucella melitensis

and Brucella suis

SOP 74, verzija 6

Serum domačih in divjih živali/ Serum of

domestic and wild animals

DA / YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

246. OIE Manual of



Animals, chapter 3.1.4. – last

version on O.I.E. web-page

Test ELISA za ugotavljanje protiteles proti

brucelam v hlevskih vzorcih mleka govedi,

SOP 494

ELISA test for the detection of Brucella

antibodies in bovine tank milk samples

Mleko / Milk NE / NO

247. Manual of Diagnostic Tests


Animals. World organisation

for animal health Chapter

3.1.3 – last version on O.I.E.

web-page

Dokaz protiteles proti virusu bolezni

modrikastega jezika z metodo ELISA –

komercialni komplet INGEZIM BTV DR

(Ingenasa)

Detection of antibodies against Bluetongue

virus by ELISA – commercial kit INGEZIM

BTV DR (Ingenasa)

SOP 364

Serum / Serum

NE / NO

248. ISO 6888-1 Preiskava na koagulaza pozitivne stafilokoke

Enumeration of coagulase-positive

staphylococci (S. aureus and other species)

by Baird-Parker agar medium

SOP 203


products

LOD = 100CFU/g

u=24,06%

NE / NO

249. ISO 6579-1:2017



prisotnosti, števila in serotipov Salmonella –

1. del: Ugotavljanje prisotnosti Salmonella

spp.



Salmonella spp.

SOP 400, verzija 2


products

LOD = 5–10 CFU/25 g


products

LOD = 5 CFU/25 g (ml)



(oz. vzorec vpojnih prevlek in brisov površin

or boot swab and surface sample)

LOD = 5–10 CFU/25 g


DA / YES

DA / YES

DA / YES

250. ISO 4833-1

Ugotavljanje skupnega števila

mikroorganizmov (SŠMO) z metodo štetja na

ploščah pri 30 oC

Enumeration of the number of

microorganisms at 30 oC by colony count

technique

SOP 206


products

LOD = 10 CFU/ml

±30,6%


products

LOD = 10 CFU/g

±37,0%

NE / NO

NE / NO

251. SOP 243


method

Preiskava na kloramfenikol v surovem mleku

z ELISA Randox testom

Determination of chloramphenicol residues in

raw milk by ELISA Randox test

presejalna metoda/screening method

Mleko / Milk

CCβ=0,27μg/kg

NE / NO

252. SIST EN ISO 8655-6 Interne kalibracije pipet – za notranje

uporabnike

V 180

Prostornina tekočin/Volume of Liquids

Pipete/Pipettes

najboljša merilna zmoglijivost/Best

measurement Capability (BMC)

NAZIVNA PROSTORNINA BMC

1 µl 0,09 µl

2 µl 0,09 µl

5 µl 0,09 µl

10 µl 0,09 µl

20 µl 0,09µl

50 µl 0,10 µl

100 µl 0,16 µl

300 µl 0,16 µl

500 µl 0,26 µl

1000 µl 0,35 µl

2000 µl 1,94 µl

5000 µl 4,60 µl

10000 µl 7,59µl

NE / NO

253. OIE Manual of Standards for

Diagnostic Tests and Vaccines

for Terrestrial Animals

(mammals, birds and bees),

poglavje 3.2.2.

Preiskava na povzročitelja hude gnilobe

čebelje zalege v medu - izolacija in

ugotavljanje števila Paenibacillus larvae


254. Larone D.H., Medically

important Fungi, A guide to

identification. Washington:

American Society for

Microbiology


dokazovanje dermatofitov v kužnini, izolacija

in determinacija dermatofitov






Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

255. OIE Manual of Standards for

Diagnostic Tests and Vaccines

for Terrestrial Animals,

Poglavje 3.1.1.

Preiskava na vranični prisad - ugotavljanje

okužbe in kontaminacije z bakterijo Bacillus

anthracis

Kri, organi, edemska tekočina, peritonealna

tekočina, koža; dlaka, volna

NE / NO

256. ISO 11290-1:2017




in Listeria spp.- 1. del: Ugotavljanje

prisotnosti





SOP 162, verzija 5

meso in mesni izdelki / meat and meat

products

LOD = 1 CFU/25 g

mleko in mlečni izdelki / milk and milk

products

LOD = 1 CFU/25 g

Ribe in ribji izdelki / Fish and fish products

LOD 1 CFU /25g


LOD = 1CFU / površina

DA / YES

DA / YES

DA / YES

DA / YES

257. ISO 11290-2

Amendment 1




in Listeria spp.- 2.del: Ugotavljanje števila



Listeria monocytogenes and of Listeria spp. -

Part 2: Enumeration method

SOP 314


products


products

NE/NO

NE/NO

258. ISO 21528-2 Mikrobiologija v prehranski verigi -


prisotnost in števila enterobakterij - 2.del:

Metoda štetja kolonij



Enterobacteriaceae - Part 2: Colony count

technique

SOP 317


products


products

NE/NO

NE/NO

259. SOP 147: 1. del

Interna metoda

SOP147: part 1

In-house method

Preiskava na bakterijo Listeria

monocytogenes - izolacija



potrditev/confirmation: glej/see SOP 147, 2.

del/part 2


pathologic samples

NE/NO

260. ISO 16649-2

Določanje števila ß glukoronidaza pozitivnih




stuffs - Horizontal method for the

enumeration of ß glucuronidase-positive

Escherichia coli


SOP 315


products



NE/NO

NE/NO




Nacionalni veterinarski inštitut, Enota Nova Gorica

Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

261. ISO 6579-1:2017

Mikrobiologija v prehranski verigi -Horizontalna






Salmonella spp.

SOP 400, verzija 2


products

LOD = (5–10) CFU/25 g


products

LOD = (5–10) CFU/25 g (ml)


products

LOD=(5-10) CFU/25 g



LOD = (5–8) CFU/25 g

(oz. vzorec vpojnih prevlek in brisov površin

or boot swab and surface sample)

Kvalitativna metoda/Qualitative method

DA / YES

DA / YES

DA / YES

DA / YES

262. ISO 11290-1:2017



Listeria monocytogenes in Listeria spp.- 1. del:

Ugotavljanje prisotnosti





SOP 162, verzija 5


products

LOD = (5–10) CFU/25 g


products

LOD = (5–10) CFU/25 g (ml)

Brisi okolja / environmental swabs

LOD = 5-10 CFU/površina

DA / YES

DA / YES

DA / YES

263. ISO 11290-2: 1998

Amendment 1: 2004

Preiskava na Listeria monocytogenes



Horizontal method for the detection of Listeria

monocytogenes

Part 2: enumeration method

SOP 314, verzija 3


products

LOD = 10 CFU/g


products

LOD = 10 CFU/g (ml)

DA / YES

DA / YES

264. ISO 4833-1: 2013


(tehnika štetja kolonij na ploščah inkubiranih pri

30 oC)


method for the enumeration of microorganisms

Part 1: Colony count at 30 oC by the pour plate

technique

SOP 206, verzija 4


products

LOD = 10 CFU/g (ml)

±11,7%


products

LOD = 10 CFU/g

±18,16%

DA / YES

DA / YES

265. ISO 16649-2:2001 Določanje števila β glukoronidaza pozitivnih







SOP 315, verzija 2


products

LOD = 10 CFU/g




LOD = 10 CFU/g (ml)

U= ± 0,07 log10CFU /ml (tekočine,liquids)

U= ± 0,10 log10CFU/g (trdne snovi, solid milk

products)

DA / YES

DA / YES

266. ISO 21528-2:2004 Preiskava na Enterobacteriaceae – števna metoda

Enumeration of Enterobacteriaceae

SOP 317, verzija 3


products

LOD = 10 CFU/g


products

LOD = 10 CFU/g (ml)

Brisi in izsečki trupov

LOD = 10 CFU /cm2


products

LOD = 10 CFU/g (ml)

DA / YES

DA / YES

DA / YES

NE / NO





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

267. ISO 10272-1: 2017

Postopek A

Procedure A



Campylobacter spp. -1.del: Metoda za

ugotavljanje prisotnosti



Campylobacter spp.


SOP 187, verzija 4


products

LOD = (5–8) CFU/10 g

DA / YES

268. ISO 10272-2 : 2017






Campylobacter spp.


SOP 385 verzija 3

Meso in mesni izdelki


LOD = 10 CFU/g

DA / YES




metoda odkrivanja

(digestivna m.)


(EC) no 2015/1375;

Annex I;




for pooled sample




method

SOP 8, verzija 9

Meso / Meat DA/YES





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No


Interna metoda

In-house method


(DELVOTEST®SP-NT)



mleko / milk


CCβ = 3 µg/kg


CCβ = 4 µg/kg


CCβ = 4 µg/kg


CCβ = 20 µg/kg


not sensitive


not sensitive


not sensitive


CCβ = 20 µg/kg


CCβ = 50 µg/kg


CCβ = 50 µg/kg


CCβ = 10 µg/kg


CCβ = 70 µg/kg






not sensitive


not sensitive

neomicin / neomycin

CCβ = 500 µg/kg






not sensitive


not sensitive


not sensitive


CCβ = 90 µg/kg















tilozin / tylosin

CCβ = 50 µg/kg



DA / YES

271. ISO 6888-1


Enumeration of coagulase-positive staphylococci

(S. aureus and other species) by Baird-Parker

agar medium

SOP 203


products

LOD = 10 CFU/g

±21,5%

NE / NO

272. IDF 108B

ISO 5764

Postopek za ugotavljanje zmrziščne točke mleka s

krioskopom

Determination of freezing point – Thermistor

cryoscope method

SOP 242

Kravje mleko / cow milk

-408 do -600 m °C

r ≤ 4 m°C

R ≤ 6 m°C

NE / NO

273. OIE Manual of Standards

for Diagnostic Tests and



Preiskava na povzročitelja hude gnilobe čebelje

zalege v medu - izolacija in ugotavljanje števila

Paenibacillus larvae






Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

274. SOP 147: 1. del

Interna metoda

SOP147: part 1

In-house method

Preiskava na bakterijo Listeria monocytogenes -

izolacija



potrditev/confirmation: glej/see SOP 147, 2.

del/part 2


pathologic samples

LOD = (5–10) CFU/25 g

NE/ NO

275. OIE Manual of




Preiskava na garjavce - dokaz z mikroskopsko

preiskavo kožnega ostružka po obdelavi s KOH

Kožni ostružek različnih vrst živali NE / NO

Nacionalni veterinarski inštitut, Enota Celje

Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

276. ISO 6579-1:2017 Mikrobiologija v prehranski verigi -Horizontalna






Salmonella spp.

SOP 400, verzija 2


products

LOD = (5–10) CFU/25 g

Feces, organi, vpojne prevleke za škornje,

stelja, vzorci živalskega okolja in površin /

Faeces, organs, boot swabs, litter, samples of

animal environment and surgaces

LOD = 9 CFU/25g

Kvalitativna metoda /Qualitative method

DA / YES

DA / YES









SOP 162, verzija 5


products

LOD = ≤8 CFU/25 g

DA / YES

278. ISO 4833-1: 2013



30 oC)




technique

SOP 206, verzija 4


products

LOD = 10 CFU/g

U= ± 0,102 log10 CFU/g.

DA / YES

279. ISO 16649-2:2001

Določanje števila β glukoronidaza pozitivnih







SOP 315, verzija 2


products:

LOD = 10 CFU/g


DA / YES




metoda odkrivanja

(digestivna m.)


(EC) no 2015/1375;

Annex I;




for pooled sample




method

SOP 8, verzija 9






Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

281. OIE Manual of



Animals,

poglavje/chapter 3.1.4,

2016

Rose bengal test za ugotavljanje protiteles proti

bakterijam Brucella abortus, Brucella melitensis

in Brucella suis


against Brucella abortus, Brucella melitensis and

Brucella suis

SOP 74, verzija 6

Serum domačih in divjih živali/ Serum of

domestic and wild animalls

DA / YES

282. ISO 6888-1 Preiskava na koagulaza pozitivne stafilokoke

Enumeration of coagulase-positive staphylococci

(S. aureus and other species) by Baird-Parker

agar medium

SOP 203


products

LOD = 100 CFU/g

U=20,18%

NE / NO





Št.

No.



Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

283. SOP 237

interna metoda/

in-house method


(DELVOTEST®SP-NT)



mleko / milk


CCβ = 3 µg/kg


CCβ = 4 µg/kg


CCβ = 4 µg/kg


CCβ = 20 µg/kg


not sensitive


not sensitive


not sensitive


CCβ = 20 µg/kg


CCβ = 50 µg/kg


CCβ = 50 µg/kg


CCβ = 10 µg/kg


CCβ = 70 µg/kg






not sensitive


not sensitive

neomicin / neomycin

CCβ = 500 µg/kg






not sensitive


not sensitive


not sensitive


CCβ = 90 µg/kg















tilozin / tylosin

CCβ = 50 µg/kg



NE / NO




Nacionalni veterinarski inštitut, Enota Kranj

Št.

No.



number

Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No


interna metoda

in-house method

Postopek ugotavljanja števila bakterij v mleku s

pomočjo fluoro-opto-elektronskega inštrumenta

Bactoscan

Enumeration of bacteria in milk with fluoro-opto-

electronic instrument Bactoscan

Surovo mleko / Raw milk

3.000–1.000.000 CFU/ml

DA / YES


interna metoda

in-house method


(DELVOTEST®SP-NT)



mleko / milk


CCβ = 3 µg/kg


CCβ = 4 µg/kg


CCβ = 4 µg/kg


CCβ = 20 µg/kg


not sensitive


not sensitive


not sensitive


CCβ = 20 µg/kg


CCβ = 50 µg/kg


CCβ = 50 µg/kg


CCβ = 10 µg/kg


CCβ = 70 µg/kg






not sensitive


not sensitive

neomicin / neomycin

CCβ = 500 µg/kg






not sensitive


not sensitive


not sensitive


CCβ = 90 µg/kg















tilozin / tylosin

CCβ = 50 µg/kg



DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No




metoda odkrivanja

(digestivna m.)


(EC) no 2015/1375;

Annex I;




for pooled sample




method

SOP 8, verzija 9


287. ISO 13366-2:2006

Ugotavljanje števila somatskih celic v mleku s

fluoro-opto-elektronskim aparatom

Enumeration of somatic cells –Fluoro-opto-

electronic method

SOP 239, verzija 5


5.000–1.500.000 somatskih celic /ml

± 7 %

DA / YES

288. IDF 108B:2009

ISO 5764:2009

Ugotavljanje zmrziščne točke mleka s krioskopom

Determination of freezing point – Thermistor

cryoscope method

SOP 242, verzija 7

Kravje mleko / Cow Milk

-408 do -600 m °C

± 2 m°C

DA / YES

289. ISO 9622:2013

(IDF 141)

Ugotavljanje vsebnosti mlečne maščobe,

beljakovin, laktoze in zmrziščne točke v mleku s

pomočjo IR spektrometra

Determination of milkfat, protein, lactose content

and freezin point with mid-infrared spectrometry

SOP 245, verzija 9


maščobe/milk fat

(2,0 do 6,0) g/100 ml

beljakovine/proteini

(2,5 do 4,5) g/100ml

laktoza/lactose

(4,0 do 5,2) g/100g

Zmrziščna točka/freezing point

(-0,449°C do -0,547°C)

DA / YES

DA / YES

DA / YES

DA / YES

DA / YES

290. ISO 6579-1:2017







Salmonella spp.

SOP 400, verzija 2


products

LOD = (<3) CFU/25 g


products

LOD = (<6) CFU/25 g (ml)





LOD = 5 CFU/25g

DA / YES

DA / YES

DA / YES


Interna metoda

In-house method

Preiskava na Salmonella spp – modificirana IRIS

metoda Horizontal method for the detection of Salmonella

spp – modified IRIS method


products

LOD = (1-5) CFU/sample 10 or 25 g


products

LOD = (1–5) CFU/25 g (ml)

Brisi trupov / Environmental swabs

LOD = (1–5) CFU/vzorec

DA / YES

292. ISO 11290-1:2017









SOP 162, verzija 5


products

LOD = < 7 CFU/25 g


products

LOD = < 7 CFU/25 g (ml)

DA / YES

DA / YES





Št.

No.



number

Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

293. ISO 16649-2:2001








SOP 315, verzija 2


products

LOD =10 CFU/g

U=±0,15 log10CFU /g


products

LOD =10 CFU/g

poltekoči mlečni izdelki (well-mixed solids);

U=±0,16 log10CFU /ml(g)

trdi mlečni izdelki (other solids)

U= ±0,12 log10CFU /g

DA / YES

DA / YES

294. ISO 4833-1:2013



30 oC)




technique

SOP 206, verzija 4


products

LOD = 10 CFU/g (ml)

±U=0,24 log10 CFU /ml

DA / YES




Nacionalni veterinarski inštitut, Enota Murska Sobota

Št.

No.



number

Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

295. ISO 6579-1:2017







Salmonella spp.

SOP 400, verzija 2


products

LOD = 5–10 CFU/25 g





LOD = 2 CFU/25g


DA / YES

DA / YES









SOP 162, verzija 5


products

LOD=5–10 CFU/25 g

DA / YES

297. ISO 4833-1: 2013



30 oC)




technique

SOP 206, verzija 4


products

LOD=10 CFU/g

U=±0,27 log10CFU /g

DA / YES

298. ISO 16649-2:2001








SOP 315, verzija 2


products:

LOD = 10 CFU/g


DA / YES

299. ISO 10272-2 : 2017






Campylobacter spp.


SOP 385 verzija 3



LOD = 10 CFU/g

± 0,36 log10 CFU/g

DA / YES




metoda odkrivanja

(digestivna m.)


(EC) no 2015/1375;

Annex I;




for pooled sample




method

SOP 8, verzija 9






Št.

No.



number

Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No


Interna metoda/

in–house method


(DELVOTEST®SP-NT)



mleko / milk


CCβ = 3 µg/kg


CCβ = 4 µg/kg


CCβ = 4 µg/kg


CCβ = 20 µg/kg


not sensitive


not sensitive


not sensitive


CCβ = 20 µg/kg


CCβ = 50 µg/kg


CCβ = 50 µg/kg


CCβ = 10 µg/kg


CCβ = 70 µg/kg






not sensitive


not sensitive

neomicin / neomycin

CCβ = 500 µg/kg






not sensitive


not sensitive


not sensitive


CCβ = 90 µg/kg















tilozin / tylosin

CCβ = 50 µg/kg



DA / YES

302. SOP 243


method

Preiskava na kloramfenikol v surovem mleku z

ELISA Randox testom

Determination of chloramphenicol residues in raw

milk by ELISA Randox test

Presejalna metoda/screening method

Mleko / Milk

CCβ=0,25μg/ml

NE / NO





Št.

No.



number

Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No

303. OIE Manual of


Vaccines for Terrestiral

Animals, chapter 3.8.3.,

2019

Dokaz protiteles proti virusu klasične prašičje

kuge z metodo ELISA –komercialni komplet



SOP 230, verzija 10

Serum prašičev /

Pork serum

DA / YES

304. OIE Manual of


Vaccines for Terrestiral

Animals, chapter 3.1.2,

2018

Dokaz protiteles proti virusu bolezni Aujeszkega z

metodo ELISA – komercialni komplet





SOP 311, verzija 6

Serum prašičev /

Pork serum

DA / YES

Nacionalni veterinarski inštitut (NVI), Gerbičeva 60, 1000 Ljubljana

Št.

No.



number

Preskusna metoda

Test method





Akreditirano* Da/Ne

Accredited Yes/No


interna metoda

in-house method

Tehnike vzorčenja za mikrobiološke preiskave živil

skladno z osnutkom ISO TS 17728, ter mleka in

mlečnih izdelkov skladno z ISO 707

Sampling techniques for microbiological analysis

of food samples according to ISO TS 17728 and

for milk and dairy products according to ISO 707

Živila živalskega izvora / Food of animal

origin

DA / YES


interna metoda

in-house method

Tehnike vzorčenja površin okolja skladno z ISO

18593 in Smernicami o vzorčenju živilsko

predelovalnih območij in opreme za detekcijo

bakterije Listeria monocytogenes

Sampling techniques for environmental surfaces

according to ISO 18593 and Guidelines on

sampling the food processing area and equipment

for the detection of Listeria monocytogenes

Vzorci okolja / Environmental samples DA / YES

307. Uredba Komisije (ES)

št. 152/2009, Uredba

Komisije (EU) št.

691/2013, Uredba

Komisije (EU) št.

142/2011, Priloge IX,

X, XIII


(EC) No 152/2009,


(EU) No 691/2013,


(EU) No 142/2011,

Annexes IX, X, XIII

Vzorčenje krme

Sampling of feed

SOP 467, verzija 2

Krma / Feed DA / YES

Documents

· Priloga B k PK VF, datum: 26.08.2020 1/79 Attachment B to PK VF, date: 26.08.2020 Seznam metod s podano merilno negotovostjo/mejami metode The list of methods with measurement