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Preventative and Therapeutic Treatment of Resveratrol on Alzheimers Disease using CL2006 and CL4176 C. elegan models Bradford Greaves

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Need http://www.searo.who.int/en/Section1174/Section1199/Section1567/Section1823_8066.htm

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The APP Processing Senile Plaques Modified from Laurn et al., Nature (2009) Figure 1 Resveratrol Promotes Clearance of Alzheimers Disease Amyloid- Peptides, Marambaud 2005 The amyloid peptide can act as a modular aggregation domain, Link 2008

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Alzheimers Disease cont. http://www.nia.nih.gov/NR/rdonlyres/A294D332-71A2-4866-BDD7-A0DF216DAAA4/0/Alzheimers_Disease_Unraveling_the_Mystery.pdf

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Oxidative Damage http://www.nia.nih.gov/NR/rdonlyres/A294D332-71A2-4866-BDD7-A0DF216DAAA4/0/Alzheimers_Disease_Unraveling_the_Mystery.pdf

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Purpose To preventatively and therapeutically treat Alzheimers Disease with resveratrol using CL2006 and CL4176 C. elegan models

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Hypothesis Alternate Hypothesis: Both preventive and therapeutic treatment of resveratrol on CL2006 will lead to a decrease in amyloid beta production and for CL4176 there will be a delay of the onset of paralysis. Null Hypothesis: There will be no change in amyloid beta production nor paralysis with the addition of resveratrol.

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Resveratrols Coloration With Alzheimers Disease http://www.chiro.org/nutrition/ABSTRACTS/Resveratrol_in_Prevention_and_Treatment.pdf

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Figure 2 Soy isoflavone glycitein protects against beta amyloid-induced toxicity and oxidative stress in transgenic Caenorhabditis elegans, Gutierrez-Zepeda 2005 Transgenic C. elegans as a Model in Alzheimers Research, Wu 2005

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Do Ability CL2006 and CL4176: Staining and Paralysis Control food and Resveratrol available Fluorescent Microscope at school

Slide 13 . Duchen, Michael R. "Roles of Mitochondria in Health and Disease." Whereas some of the genes identified in the control of longevity appear to target mitochondria or at least to alter antioxidant defenses of the cell (Duchen, 2004. 2004. Diabetes. 7 May 2009. Halliwell, Barry, and John M.C. Gutteridge. Free Radicals in Biology and Medicine. 3rd ed. New York: Oxford UP, 2004. Lagouge, Marie, Carmen Argmann, Zachary Gerhart-Hines, Hamid Meziane, Carles Lerin, Frederic Daussin, Nadia Messadeq, Jill Milne, Philip Lambert, Peter Elliott, Bernard Geny, Markku Laakso, Pere Puigserver, and Johan Auwerx. "Resveratrol Improves Mitochondrial Function and Protects Against Metabolic Disease by Activating SIRT1 and PGC-1a." Cell (2006). 20 May 2008. Lenker, David P. Professional Guide to Diseases. 8th ed. New York: Williams & Wilkins., 2005 Marambaud, Phillipe. Wine Compound May Help Cut Risk of Alzheimers Disease. 16 Nov. 2005. 24 Apr. 2009.http://www.sciencedaily.com/releases/2008/06/080617165716.htm>. McKenzie, Maureen A. "Insulin Receptor-Like Protein." Patent Storm. 5 Oct. 1992. 18 Apr. 2008. O,Neill, Bill. "In Methuselahs Mould." Feature 2 (2004): 0014-017. PLoS Biology..http://www.culturagalega.org/imaxes/docs/plos_lonxevidade.pdf>. "Resveratrol Studies Suggest Broad Implications for SIRT1 Activation,in Neurodegenerative Diseases of Aging Such as Alzheimer's Disease ( whether any of the Company's product ca...)." Bio-Medicine - latest biology and medical news/technology. 26 June 2007. 29 Apr. 2009. Rogers, Kara, ed. "Alzheimer's Disease." Encyclopdia Britannica. 15th ed. 2002. 306-07. Society for Neuroscience. "Grape Seed Extract May Reduce Cognitive Decline Associated With Alzheimer's Disease." ScienceDaily 18 June 2008. 29 April 2009.">

Bibliography Bundschuh, K. "The worm in humans." 11 Nov. 2008. Biotechnology and Life Sciences in Baden-Wrttemberg. 6 May 2009. Cheng, Louse. Compound in Red Wine May Fight Alzeimers. WebMD. 4 Nov. 2005. 15 Apr. 2009.http://www.webmd.com/alzheimers/news/20051104/compound-in-red-wine-may-fight-alzheimer>. Duchen, Michael R. "Roles of Mitochondria in Health and Disease." Whereas some of the genes identified in the control of longevity appear to target mitochondria or at least to alter antioxidant defenses of the cell (Duchen, 2004. 2004. Diabetes. 7 May 2009. Halliwell, Barry, and John M.C. Gutteridge. Free Radicals in Biology and Medicine. 3rd ed. New York: Oxford UP, 2004. Lagouge, Marie, Carmen Argmann, Zachary Gerhart-Hines, Hamid Meziane, Carles Lerin, Frederic Daussin, Nadia Messadeq, Jill Milne, Philip Lambert, Peter Elliott, Bernard Geny, Markku Laakso, Pere Puigserver, and Johan Auwerx. "Resveratrol Improves Mitochondrial Function and Protects Against Metabolic Disease by Activating SIRT1 and PGC-1a." Cell (2006). 20 May 2008. Lenker, David P. Professional Guide to Diseases. 8th ed. New York: Williams & Wilkins., 2005 Marambaud, Phillipe. Wine Compound May Help Cut Risk of Alzheimers Disease. 16 Nov. 2005. 24 Apr. 2009.http://www.sciencedaily.com/releases/2008/06/080617165716.htm>. McKenzie, Maureen A. "Insulin Receptor-Like Protein." Patent Storm. 5 Oct. 1992. 18 Apr. 2008. O,Neill, Bill. "In Methuselahs Mould." Feature 2 (2004): 0014-017. PLoS Biology..http://www.culturagalega.org/imaxes/docs/plos_lonxevidade.pdf>. "Resveratrol Studies Suggest Broad Implications for SIRT1 Activation,in Neurodegenerative Diseases of Aging Such as Alzheimer's Disease ( whether any of the Company's product ca...)." Bio-Medicine - latest biology and medical news/technology. 26 June 2007. 29 Apr. 2009. Rogers, Kara, ed. "Alzheimer's Disease." Encyclopdia Britannica. 15th ed. 2002. 306-07. Society for Neuroscience. "Grape Seed Extract May Reduce Cognitive Decline Associated With Alzheimer's Disease." ScienceDaily 18 June 2008. 29 April 2009.