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Electronic Journal of Biotechnology 17 (2014) 14 – 18

Production and characterization of algae extract fro !hlaydoona" #einhardtii

Weston Kightlinger, Kai Chen, Azadeh Pourmir, Daniel W. Crunkleton, Geoffrey L. Price, Tyler W. ohannes

De!artment of Chemical "ngineering, The #ni$ersity of Tulsa, %&& 'outh Tucker Dri$e, Tulsa, (K )*+&*,

#'A

Pre"ented $y %

&r'an"yah Putra

Po"t raduate Progra ndala" *ni+er"ity

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Effect" of algae extract on $acterial eta$oli"

ero$ic gro'th of E. coli  on

,B ediu containing - g.,

of either yea"t extract (/E)

or algae extract (E) Each

ea"ureent rere"ent" the

ean of trilicateexerient" "tandard

de+iation"

3ecific gro'th rate" and rotein roduction fro E. coli 

 gro'n on ,B ediu containing - g., of either yea"textract (/E) or algae extract (E) Each ea"ureent rere"ent" the ean of trilicate exerient" "tandard

de+iation"

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Effect" of algae extract on yea"t eta$oli"

naero$ic gro'th

of S. cerevisiae on

/P ediu

containing 10 g.,

of either yea"t

extract (/E) or

algae extract

(E) Each

ea"ureent

rere"ent" the

ean of trilicate

exerient" "ta

ndard de+iation"

3ecific gro'th rate" and ethanol roduction fro S. cerevisiae

 gro'n on /P ediu containing 10 g., of eitheryea"t extract (/E) or algae extract (E) Each ea"ureent rere"ent" the ean of trilicate

exerient" "tandard de+iation"

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Concluding remarks

  Algae e-tract !roduced greater groth and meta/olism saturation $alues as ell

as !rolonged groth and !rotein !roduction in com!arison to yeast e-tract

hen used in com!le- media reci!es for ". Coli cultures. When used as a

com!le- media su!!lement in '. Cere$isiae cultures, algae e-tract !roducedsu!erior groth and com!ara/le ethanol !roduction rates and saturation $alues.

The !rolonged groth !eriod afforded to yeast and /acteria cultures gron in

algae e-tract may /e useful for some enzyme e-!ression systems or secondary

meta/olite !roduction.

left to right: asks containing the same concentration of Chlamydomonasreinhardtii (i) wild strain CC 125 and mutants* (ii) tla1, (iii) 3!"1#tla1, (i$)

tla%&*