Preparation of competent E. coli cells

Preparation of probes by PCR

Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV

Purification of the digested fragment

Ligation of fragments with pBR322 plasmid

Transformation of the ligated construct into pBR322

Selection of recombinant E. coli cells

Isolation and purification of the pBR322 plasmid with insert

Digestion of pBR322 with restriction enzymes

.Identification of inserts by electrophoreses and Southern blotting

Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV

Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV

Purification of the digested fragment

Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV

Purification of the digested fragment

Ligation of fragments with pBR322 plasmid

Preparation of competent E. coli cells

Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV

Purification of the digested fragment

Ligation of fragments with pBR322 plasmid

Transformation of the ligated construct into pBR322

Preparation of competent E. coli cells

Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV

Purification of the digested fragment

Ligation of fragments with pBR322 plasmid

Transformation of the ligated construct into pBR322

Selection of recombinant E. coli cells

Preparation of competent E. coli cells

Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV

Purification of the digested fragment

Ligation of fragments with pBR322 plasmid

Transformation of the ligated construct into pBR322

Selection of recombinant E. coli cells

Isolation and purification of the pBR322 plasmid with insert

Preparation of competent E. coli cells

Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV

Purification of the digested fragment

Ligation of fragments with pBR322 plasmid

Transformation of the ligated construct into pBR322

Selection of recombinant E. coli cells

Isolation and purification of the pBR322 plasmid with insert

Digestion of pBR322 with restriction enzymes

Preparation of competent E. coli cells

Preparation of probes by PCR

Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV

Purification of the digested fragment

Ligation of fragments with pBR322 plasmid

Transformation of the ligated construct into pBR322

Selection of recombinant E. coli cells

Isolation and purification of the pBR322 plasmid with insert

Digestion of pBR322 with restriction enzymes

.Identification of inserts by electrophoreses and Southern blotting

Preparation of competent E. coli cells

Preparation of probes by PCR

Digestion of pBR322 and plasmid with restrionenzymes BamH1, EcoR1 and EcoRV

Purification of the digested fragment

Ligation of fragments with pBR322 plasmid

Transformation of the ligated construct into pBR322

Selection of recombinant E. coli cells

Isolation and purification of the pBR322 plasmid with insert

Digestion of pBR322 with restriction enzymes

.Identification of inserts by electrophoreses and Southern blotting