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Genetic Transformation
What is transformation? When a cell takes up and
expresses a new piece of genetic material—DNA— in order to change the organism’s traits
A gene is a piece of DNA which provides the instructions for making (coding for) a particular protein
DNA Protein Trait
History of Transformation 1928 – Fredrick Griffth first
described transformation Pneumococcus bacteria
(pneumonia) and mice 1944 – Oswald Avery,
Colin MacLeod, Maclyn McCarty identified the “transforming principle” as DNA
History of Transformation
1952 – Alfred Hershey and Martha Chase provided final proof that DNA was the molecule of heredity
1970 – Morton Mandel and Akiko Higa developed a protocol for transforming E. coli bacteria
What is transformation used for? Agricultural
Genes coding for traits such as frost, pest or drought resistance can be genetically transformed into plants
What is transformation used for? Environmental
Bacteria can be genetically transformed with genes enabling them to digest oil spills or remove pollutants from the environment
What is transformation used for? Medical
Production of human proteins to treat genetic diseases Protein Disease/
DisorderHuman insulin Diabetes mellitusHuman Growth Hormone
Deficiency in children
Erythropoietin AnemiaDNase I Cystic fibrosisHuman antibody blocker
Asthma
What are we doing? We will transform bacteria (E. coli),
giving it the ability to make green fluorescent proteins
Green Fluorescent Protein Used in science as a visual
marker… Biological processes (protein
production) Localization and regulation of gene
expression Cell movement Cell fate during development Formation of different organs Marker to identify transgenic
organisms
How does it work?
*plasmids*
Bacterial chromosomal DNA
Cell membrane
Making Plasmids
Gene
Plasmid
+=
pGLO Plasmid
GFP
araC
To make green fluorescent proteins
To survive with Ampicillin (antibiotic)