Population genetics of the Population genetics of the wheat leaf rust fungus, wheat leaf rust fungus,

Puccinia triticinaPuccinia triticinain Central Asiain Central Asia

J. A. KolmerJ. A. Kolmer

M. E. OrdonezM. E. Ordonez

USDA-ARS USDA-ARS

Cereal Disease LaboratoryCereal Disease Laboratory

University of Minnesota -St. PaulUniversity of Minnesota -St. Paul

Leaf Rust CollectionsLeaf Rust Collections

Collections from 2002, Collections from 2002, 2004, 20052004, 2005

A. A. Morgounov - CIMMYT, Morgounov - CIMMYT, Almaty KazakhstanAlmaty Kazakhstan

137 isolates tested for 137 isolates tested for virulence - 99 for SSRvirulence - 99 for SSR

Caucasus - (Azb.,Geo. Caucasus - (Azb.,Geo. Arm.) 13 Uzbekistan - 7 Arm.) 13 Uzbekistan - 7

Tajikistan-Kyrgyzstan -15 Tajikistan-Kyrgyzstan -15 S. Kazakhstan - 39 S. Kazakhstan - 39 N. Kazakhstan- 25 N. Kazakhstan- 25 N. America-16 N. America-16

Durum - 2Durum - 2

Clone corrected within Clone corrected within populations populations

(race/SSR genotype)(race/SSR genotype) 20 differentials - 23 SSR 20 differentials - 23 SSR

lociloci

Super highway - Kazakhstan

Virulence and SSR DiversityVirulence and SSR Diversity

Cauc.Cauc. N. Kaz.N. Kaz. S. KazS. Kaz Tjk-KygTjk-Kyg UzbekUzbek N. Am.N. Am.

No. No. isolatesisolates 1313 2525 3939 1515 77 1616

No. No. racesraces 1212 2121 3737 1515 77 1616

No. SSR No. SSR

genotypgenotypee 1111 2121 3434 99 55 1414

ShannoShannon/ n/ Ln(N)Ln(N) 0.920.92 0.930.93 0.940.94 0.700.70 0.760.76 0.930.93

Tjk-Kyg-Uzb.-Tjk-Kyg-Uzb.-Caucasus not sig. Caucasus not sig. differentiated differentiated

S. Kaz - N. Kaz.S. Kaz - N. Kaz.not sig. not sig. differentiateddifferentiated

Differentiation Statistics (Phi) of Pair-wise Populations - Virulence phenotypes

Neighbor joining

Tjk-Kyg-Uzb - Caucasus not signif. differentiated

S. Kaz - N. Kaz. not signif. differentiated

0.82 correlation with virulence tree

Differentiation (Fst) Statistics of Pair-wise Populations SSR Genotypes

Neighbor joining

-Central Asia1 Avir Lr2a Vir Lr2c24Most from Cauc-Tjk-Kry-Uzb.

3 Vir Lr2a Vir Lr2cMost from S.+ N.Kazakhstan

-North America5 Avir Lr2a Vir Lr2c7 Vir Lr2a Lr2c Avir Lr2a Lr2c

-Durum 6 Avir. to almost all Lr genes

Individual Isolates SSR Allele Variation

Neighbor joining

Central AsiaCentral AsiaP. triticinaP. triticina populations populations

Two distinct Two distinct regional regional populations - populations - mountain barrier mountain barrier

Limited migration Limited migration between regions - between regions - very few SSR-very few SSR-virulence virulence genotypes in genotypes in commoncommon

Strong relationship Strong relationship between virulence between virulence and SSR genotypesand SSR genotypes

Come to Kazakhstan!!!

Population Genetics of Population Genetics of P. triticina - P. triticina - Future GoalsFuture Goals

Describe world-wide Describe world-wide populations populations

20 Tc line differentials20 Tc line differentials

23 SSR loci23 SSR loci

• North America, Central North America, Central Asia, Middle East, Asia, Middle East,

South America, EuropeSouth America, Europe

• Determine genetic Determine genetic relationships between relationships between populations - populations -

(MCDS - USA- S. America)(MCDS - USA- S. America)