15
E. Orlova E Orlo ov ov v va v va a a Lecture 9 Image processing for cryo microscopy Sep 5 - Sept 15, 2017 Birkbeck College London Practical Course Point group symmetries Symmetry in plane Symmetry in 2D space Symmetry in 3D space Point group symmetries Symmetry in space symmetry in projections WHAT IS SYMMETRY The term Symmetry has Greek origins. For the Greeks it meant the harmony of parts , proportions and rhythm. Another more mathematical way, that is related to our image analysis and crystallography is: An object can be divided by a point, or line, or radiation lines or planes into to or more parts EXACTLY SIMILAR in size and shape, and in position relative to the dividing element. Repetition of exactly similar parts facing each other or a centre. Why is it Important? We can classify molecules (biocomplexes) according to the level of their symmetry elements, so they can be grouped together having the same set of symmetry elements. This classification is very important, because it allows to make some general conclusions about molecular properties without extra calculation. On the atomic level it helps to reveal the molecular properties without any calculations. We will be able to decide if a molecule has a dipole moment or not , and to know how these property are reflected on their surfaces. What sort of interaction hold biomolecules together in huge biocomlexes such as viruses, secretion systems, etc. Symmetry around us

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Page 1: Point group symmetries Why is it Important? reveal the

E. O

rlova

E

Orloo

vovvvavvaaa

Lect

ure 9

Imag

e pr

oces

sing

fo

r cry

o m

icro

scop

y Se

p 5

- Sep

t 15,

201

7 Bi

rkbe

ck C

olle

ge

Lond

on

Pra

ctic

al C

ours

e

Poin

t gro

up s

ymm

etrie

s

Sym

met

ry in

pla

ne

Sym

met

ry in

2D

spa

ce

Sym

met

ry in

3D

spa

ce

Po

int g

roup

sym

met

ries

Sym

met

ry in

spa

ce –

sym

met

ry in

pro

ject

ions

WH

AT IS

SYM

MET

RY

The

term

Sym

met

ry h

as G

reek

orig

ins.

For

the

Gre

eks

it m

eant

the

harm

ony

of p

arts

, pr

opor

tions

an

d rh

ythm

. An

othe

r mor

e m

athe

mat

ical

way

, tha

t is

rela

ted

to

our i

mag

e an

alys

is a

nd c

ryst

allo

grap

hy is

: An

obj

ect c

an b

e di

vide

d by

a p

oint

, or l

ine,

or

radi

atio

n lin

es o

r pla

nes

into

to o

r mor

e pa

rts

EXA

CTL

Y SI

MIL

AR

in s

ize

and

shap

e, a

nd in

po

sitio

n re

lativ

e to

the

divi

ding

ele

men

t. R

epet

ition

of

exa

ctly

sim

ilar p

arts

faci

ng e

ach

othe

r or a

cen

tre.

Why

is it

Impo

rtan

t?

We

can

clas

sify

mol

ecul

es (b

ioco

mpl

exes

) acc

ordi

ng to

the

leve

l of t

heir

sym

met

ry e

lem

ents

, so

they

can

be

grou

ped

toge

ther

hav

ing

the

sam

e se

t of s

ymm

etry

ele

men

ts.

This

cla

ssifi

catio

n is

ver

y im

porta

nt, b

ecau

se it

allo

ws

to

mak

e so

me

gene

ral c

oncl

usio

ns a

bout

mol

ecul

ar p

rope

rties

w

ithou

t ext

ra c

alcu

latio

n.

On

the

atom

ic le

vel i

t hel

ps to

reve

al th

e m

olec

ular

pro

perti

es

with

out a

ny c

alcu

latio

ns. W

e w

ill be

abl

e to

dec

ide

if a

mol

ecul

e ha

s a

dipo

le m

omen

t or

not

, an

d to

kno

w h

ow

thes

e p

rope

rty a

re re

flect

ed o

n th

eir s

urfa

ces.

Wha

t sor

t of

inte

ract

ion

hold

bio

mol

ecul

es to

geth

er in

hug

e bi

ocom

lexe

s su

ch a

s vi

ruse

s, s

ecre

tion

syst

ems,

etc

.

Sym

met

ry

arou

nd u

s

Page 2: Point group symmetries Why is it Important? reveal the

We

say

that

suc

h an

obj

ect i

s sy

mm

etric

with

re

spec

t to

a gi

ven

oper

atio

n if

this

ope

ratio

n,

whe

n ap

plie

d to

the

obje

ct, d

oes

not a

ppea

r to

cha

nge

it.

Sym

met

ry m

ay d

epen

d on

the

prop

ertie

s un

der c

onsi

dera

tion:

fo

r an

imag

e w

e m

ay c

onsi

der j

ust t

he

shap

es, o

r als

o th

e co

lors

; fo

r an

obje

ct (3

D),

we

may

add

ition

ally

co

nsid

er d

ensi

ty, c

hem

ical

com

posi

tion,

co

ntac

ts b

etw

een

dom

ains

, etc

.

Bio

logi

cal o

bjec

ts

The

pers

on -

here

afte

r ref

erre

d to

as

‘it’ h

as a

mirr

or p

lane

, pr

ovid

ed it

sta

nds

stra

ight

(and

th

at w

e ig

nore

its

inte

rnal

or

gans

). Ea

ch h

alf o

f the

figu

re is

an

asy

mm

etric

uni

t.

Mov

ing

an a

rm o

r leg

des

troys

th

e sy

mm

etry

and

the

who

le

figur

e ca

n th

en b

e tre

ated

as

an

asym

met

ric u

nit.

Th

is li

ttle

‘obj

ect’,

bot

h w

ith a

nd

with

out i

ts m

irror

pla

ne w

ill be

us

ed to

illu

stra

te fu

rther

sy

mm

etry

ele

men

ts, a

nd to

bu

ild m

ore

com

plic

ated

gro

ups.

1D

2D

3D

Ope

ratio

ns w

ith M

ultim

eric

Fun

ctio

ns

One

dim

ensi

onal

func

tion

-> a

cur

ve

1 5

2 3

4 6

10

7 8

9 11

12

14

13

15

1 5

2 3

4 6

10

7 8

9 11

12

14

13

15

Int

Int

X X X

Ther

e ar

e tw

o ty

pes

oper

atio

ns w

ith fu

nctio

ns in

on

e-di

men

sion

al s

pace

.

1. T

rans

latio

n

1D

2D

3D

)a

x(g)x(g

a g(

x)

g(x+

a)

a g(

x+2a

)

Ope

ratio

ns w

ith M

ultim

eric

Fun

ctio

ns

Page 3: Point group symmetries Why is it Important? reveal the

2.

Ref

lect

ion

(mirr

or)

Thes

e o

pera

tions

can

be

com

bine

d

)xb(g

)x(g

b

a

)xa

b(g)

ax(g

)a

x(g

)x(g

11

)xb(g

)x(g)x(

g1

Ope

ratio

ns w

ith M

ultim

eric

Fun

ctio

ns

Ther

e ar

e th

ree

type

s op

erat

ions

with

im

ages

in p

lane

: 1.

Tran

slat

ion

in tw

o di

rect

ions

2.

Ref

lect

ion

3.

Rot

atio

n

in p

lane

1D

2

D

3D

Ope

ratio

ns w

ith M

ultim

eric

Fun

ctio

ns

Tran

slat

ion

in O

NE

dire

ctio

n

)a

r(g)

r(g

a

Ope

ratio

ns w

ith M

ultim

eric

Fun

ctio

ns

Tran

slat

ion

in tw

o di

rect

ions

a b

)bl

akr(g

)r(g

Ope

ratio

ns w

ith M

ultim

eric

Fun

ctio

ns

Page 4: Point group symmetries Why is it Important? reveal the

Ref

lect

ion

= m

irror

)

ra(g

)r(g

Ther

e is

a p

lain

bet

wee

n ob

ject

s. S

o th

e di

stan

ce fr

om a

ny p

oint

of

the

obje

ct a

nd th

e pl

ain

is th

e sa

me

as th

e di

stan

ce b

etw

een

the

sam

e po

int o

f the

mirr

ored

obj

ect a

nd th

e pl

ain

Ope

ratio

ns w

ith M

ultim

eric

Fun

ctio

ns

T T

M

irror

-imag

e sy

mm

etry

Th

is is

the

mos

t fam

iliar a

nd

conv

entio

nally

taug

ht ty

pe o

f sy

mm

etry

. It a

pplie

s fo

r ins

tanc

e

for t

he le

tter T

: whe

n th

is le

tter i

s re

flect

ed a

long

a v

ertic

al a

xis,

it

appe

ars

the

sam

e. T

has

a

verti

cal s

ymm

etry

axi

s.

A re

flect

ion

"flip

s" a

n ob

ject

ove

r a

line

(in 2

D) o

r pla

ne (i

n 3D

), in

verti

ng

it to

its

mirr

or im

age,

as

if in

a m

irror

. If

the

resu

lt is

the

sam

e th

en w

e ha

ve m

irror

-imag

e sy

mm

etry

(als

o kn

own

in th

e te

rmin

olog

y of

mod

ern

phys

ics

as P

-sym

met

ry).

T

T

Ope

ratio

ns w

ith M

ultim

eric

Fun

ctio

ns

Ref

lect

ion

(mirr

or o

pera

tion)

Tr

ansl

atio

n

Shift

and

refle

ctio

n

Ope

ratio

ns w

ith M

ultim

eric

Fun

ctio

ns

73o

Rot

atio

n in

pla

ne

Ope

ratio

ns w

ith M

ultim

eric

Fun

ctio

ns

Page 5: Point group symmetries Why is it Important? reveal the

Inve

rsio

n

g(r)

=g(-r

)

Ther

e is

a p

oint

bet

wee

n ob

ject

s. S

o th

e di

stan

ces

from

any

po

int o

f the

obj

ect a

nd th

e po

int a

re th

e sa

me

as th

e di

stan

ces

betw

een

the

sim

ilar p

oint

s of

the

inve

rsed

obj

ect a

nd th

e po

int

Ope

ratio

ns w

ith M

ultim

eric

Fun

ctio

ns

http

s://r

eadi

ngfe

ynm

an.o

rg/ta

g/p-

sym

met

ry/

Rot

atio

nal s

ymm

etry

Cn

A ro

tatio

n ro

tate

s an

obj

ect a

bout

a p

oint

(in

3D: a

bout

an

axis

). R

otat

iona

l sym

met

ry o

f ord

er n

, als

o ca

lled

n-fo

ld

rota

tiona

l sym

met

ry, w

ith re

spec

t to

a pa

rticu

lar p

oint

or a

xis

mea

ns th

at ro

tatio

n by

an

angl

e of

360

/n d

oes

not c

hang

e th

e ob

ject

.

(180

, 120

, 90

, 72

, 60

, 51.

43,4

5, 4

0 …

..

2

,

3,

4

, 5

, 6

,

7,

8

, 9

et

c )

For e

ach

poin

t or a

xis

of s

ymm

etry

the

sym

met

ry g

roup

is

isom

etric

with

the

cycl

ic g

roup

Cn o

f ord

er n

. The

fu

ndam

enta

l dom

ain

is a

sec

tor o

f 360

/n.

C

2,

C

3, C

4, C

5, C

6,

C

7,

C8,

C9

One

poi

nt re

mai

ns u

nmov

ed, w

hich

is th

e ro

tatio

n po

int

Rot

atio

nal s

ymm

etry

2-fo

ld

3-fo

ld

5-fo

ld

6-fo

ld

C2

C3

C5

C6

Sym

met

ry c

ombi

natio

ns

Mor

e co

mpl

ex s

ymm

etrie

s ar

e co

mbi

natio

ns o

f re

flect

iona

l, ro

tatio

nal,

trans

latio

nal,

and

glid

e re

flect

ion

sym

met

ry.

Mirr

or-im

age

sym

met

ry in

com

bina

tion

with

n-fo

ld

rota

tiona

l sym

met

ry, w

ith th

e po

int o

f sym

met

ry o

n th

e lin

e of

sym

met

ry, i

mpl

ies

mirr

or-im

age

sym

met

ry w

ith re

spec

t to

line

s of

refle

ctio

n ro

tate

d by

mul

tiple

s of

180

/n, i

.e. n

re

flect

ion

lines

whi

ch a

re ra

dial

ly s

pace

d ev

enly

(for

odd

n

this

alre

ady

follo

ws

from

app

lyin

g th

e ro

tatio

nal s

ymm

etry

to

a s

ingl

e re

flect

ion

axis

, but

it a

lso

hold

s fo

r eve

n n)

.

Page 6: Point group symmetries Why is it Important? reveal the

Com

bina

tion

of s

ymm

etrie

s in

pla

ne

P3

Com

bina

tion

of s

ymm

etrie

s in

pla

ne

P3

(from

R. H

ende

rson

)

The

five

basi

c la

ttice

type

s Th

ere

are

17 s

pace

gro

ups

in th

e pl

ane,

but

thei

r uni

t ce

lls fa

ll in

to o

ne o

f fiv

e ba

sic

shap

es a

s fo

llow

s:

repe

atin

g pa

ttern

s in

the

plan

e ca

n on

ly h

ave

1, 2

, 3, 4

or

6-fo

ld s

ymm

etry

. In

parti

cula

r, re

peat

ing

patte

rns

in th

e pl

ane

cann

ot h

ave

five-

fold

sym

met

ry.

W

hat i

s ab

out f

ive-

fold

sym

met

ry?

The

elem

ent o

f the

uni

t cel

l may

hav

e 5-

fold

sym

met

ry

but t

he o

vera

ll sy

mm

etry

of t

he p

atte

rn d

oes

not.

In

rece

nt y

ears

som

e m

ater

ials

hav

e tu

rned

up

with

fiv

e-fo

ld s

ymm

etry

. The

se m

ater

ials

, ter

med

qu

asic

ryst

als,

do

not h

ave

repe

atin

g pa

ttern

s.

http

://w

ww.

spsu

.edu

/mat

h/til

e/sy

mm

/iden

t17.

htm

Page 7: Point group symmetries Why is it Important? reveal the

Com

bina

tion

of s

ymm

etrie

s in

pla

ne

Prot

eins

don

’t do

this

– p

ack

by tr

ansl

atio

ns

Sym

met

ry in

3D

spa

ce

Thre

e ty

pes

oper

atio

ns w

ith o

bjec

ts in

spa

ce

Tran

slat

ion

in th

ree

dire

ctio

ns

Ref

lect

ion

(mirr

or)

in s

pace

R

otat

ion

(In

vers

ion

)

Rot

atio

ns c

an b

e co

mbi

ned

with

tran

slat

ions

an

d re

flect

ions

, how

ever

they

can

not b

e co

mbi

ned

in a

n ar

bitra

ry w

ay.

Sym

met

ry o

pera

tors

impo

se c

onst

rain

s.

Com

bina

tion

of o

pera

tors

can

gen

erat

e in

finite

latti

ces

as w

e ca

n se

e th

at in

cry

stal

s.

Basi

c sy

mm

etry

ope

ratio

ns in

spa

ce Tran

slat

ion

in

thre

e di

rect

ions

Unit

cell

a

B A

C

Cry

stal

l

Page 8: Point group symmetries Why is it Important? reveal the

Sym

met

ry in

3D

spa

ce

One

poi

nt re

mai

ns u

ncha

nged

. Th

ere

are

no tr

ansl

atio

nal o

pera

tors

C

ombi

natio

n of

rota

tion,

mirr

or a

nd in

vers

ion

give

s 32

com

bina

tions

Bu

t for

the

prot

eins

we

will

have

onl

y 11

co

mbi

natio

ns: n

o in

vers

ion

or m

irror

A sp

ace

rela

tions

hip

bet

wee

n el

emen

ts in

eac

h ol

igom

eric

mol

ecul

e ca

n be

des

crib

ed b

y a

set

sym

met

ry o

pera

tions

tha

t de

scrib

es t

he o

vera

ll m

olec

ular

sy

mm

etry

. Th

is

com

bina

tion

of

oper

atio

ns d

efin

e th

e PO

INT

GR

OU

P of

the

m

olec

ule.

Basi

c sy

mm

etry

ope

ratio

ns in

spa

ce

Mirr

or p

lane

Mirr

or p

lane

, sho

wn

as

dash

ed li

ne

Com

bina

tion

of

two-

fold

axi

s with

m

irror

pla

nes

Mirr

or p

lane

Ref

lect

ion

(mirr

or)

in

spa

ce

Rota

tions

axi

s

Basi

c sy

mm

etry

ope

ratio

ns in

spa

ce

Rot

atio

n

in s

pace

Mirr

or p

lane

Mirr

or p

lane

Basi

c sy

mm

etry

ope

ratio

ns in

spa

ce

The

grou

p do

es n

ot fo

rm a

mirr

or p

lane

Ref

lect

ion

(mirr

or)

in s

pace

R

otat

ion

in

spa

ce

C2

Cm

Page 9: Point group symmetries Why is it Important? reveal the

In th

ree

dim

ensi

ons

we

can

dist

ingu

ish

cylin

dric

al

sym

met

ry a

nd s

pher

ical

sym

met

ry (n

o ch

ange

whe

n ro

tatin

g ab

out o

ne a

xis,

or f

or a

ny ro

tatio

n).

Ther

e is

no

depe

nden

ce o

n th

e an

gle

usin

g cy

lindr

ical

coo

rdin

ates

and

no

depe

nden

ce o

n ei

ther

an

gle

usin

g sp

heric

al c

oord

inat

es.

Cyl

indr

ical

sym

met

ry is

ver

y of

ten

calle

d as

cyc

lical

sy

mm

etry

. Th

e si

mpl

est s

ymm

etry

is C1,

if th

e ob

ject

doe

s no

t ha

ve a

ny s

ymm

etry

at a

ll.

Sym

met

ry in

3D

spa

ce

Bas

ic s

ymm

etry

ope

ratio

ns in

spa

ce

Rota

tion

axi

s

Mirr

or

plan

e

Inve

rsio

n ce

ntre

– ro

tatio

n ce

ntre

C2m

http

://cs

i.che

mie

.tu d

arm

stad

t.de/

ak/im

mel

/tuto

rials

/sym

met

ry/in

dex4

.htm

l

Basi

c sy

mm

etry

ope

ratio

ns in

spa

ce

Cn

(for c

yclic

) ind

icat

es th

at th

e gr

oup

has

an n

-fold

ro

tatio

n ax

is..

Sn (f

or S

pieg

el, G

erm

an fo

r mirr

or) d

enot

es a

gro

up

that

con

tain

s on

ly a

n n-

fold

rota

tion-

refle

ctio

n ax

is.

D (

for d

ihed

ral,

or tw

o-si

ded)

indi

cate

s th

at th

e gr

oup

has

an n

-fold

rota

tion

axis

plu

s a

two-

fold

axi

s pe

rpen

dicu

lar t

o th

at a

xis.

In c

ryst

allo

grap

hy th

is a

pplie

s on

ly fo

r n =

1, 2

, 3, 4

, 6,

due

to th

e cr

ysta

llogr

aphi

c re

stric

tion

theo

rem

.

Page 10: Point group symmetries Why is it Important? reveal the

sym

met

ry C

2

Rot

atio

nal s

ymm

etry

is C

n, if

the

obje

ct h

as s

ever

al e

lem

ents

, tha

t arra

nged

in

a c

ircul

ar s

yste

m. T

he n

umbe

r of e

lem

ents

det

erm

ines

the

orde

r of

sym

met

ry.

Proj

ectio

ns

Sym

met

ry in

3D

spa

ce

sym

met

ry C

3

C 2 C 3

Proj

ectio

ns o

f the

obj

ect

rota

tiona

l sy

mm

etry

and

thei

r sym

met

ry.

Sym

met

ry in

3D

spa

ce

a-La

troto

xin

52

0kD

a C

a re

leas

e ch

anne

l 2.

4 M

da

C14

C13

C

4

Porta

l pro

tein

SPP

1

C5

Rot

atio

nal s

ymm

etry

Sy

mm

etry

in 3

D s

pace

sy

mm

etry

D2

Sym

met

ry in

3D

spa

ce

Page 11: Point group symmetries Why is it Important? reveal the

Dih

edra

l poi

nt g

roup

sym

met

ry D

n ar

e a

com

bina

tion

of c

yclic

al

sym

met

ries

with

a tw

o-fo

ld a

xis,

whi

ch is

per

pend

icul

ar to

the

axis

of

rota

tion.

22

2 D

2

32

D3

52

D5

Sym

met

ry in

3D

spa

ce

D3

Pal

iniru

s el

epha

s he

moc

yani

n (7

5kD

a x

6)

D5

Ke

yhol

e Li

mpe

t Hem

ocye

anin

Plat

o an

d Ar

istot

le

Plat

onic

and

Ar

chim

edea

n Po

lyhe

dra

Th

e Pl

aton

ic S

olid

s, d

isco

vere

d by

the

Pyth

agor

eans

but

de

scrib

ed b

y Pl

ato

(in th

e Ti

mae

us) a

nd u

sed

by h

im fo

r his

th

eory

of t

he 4

ele

men

ts, c

onsi

st o

f sur

face

s of

a s

ingl

e ki

nd

of re

gula

r pol

ygon

, with

iden

tical

ver

tices

. Th

e Ar

chim

edea

n So

lids,

con

sist

of s

urfa

ces

of m

ore

than

a

sing

le k

ind

of re

gula

r pol

ygon

, with

iden

tical

ver

tices

and

id

entic

al a

rrang

emen

ts o

f pol

ygon

s ar

ound

eac

h po

lygo

n.

Cub

ic p

oint

grou

p sy

mm

etrie

s T

- 2

3 T

etra

hedr

al s

ymm

etry

requ

eire

s a

min

imum

of 1

2 id

entic

al s

ubun

its

O -

432

Oct

ahed

ral p

oint

gro

uop

sym

met

ry,

ne

eds

24 s

ubun

its

I -

532

Ico

sahe

dral

sym

met

ry, 6

0 su

buni

ts

Page 12: Point group symmetries Why is it Important? reveal the

Tetra

hedr

on

3

2 3

3

2

3

Com

bina

tion

of s

ymm

etrie

s in

3D

Com

bina

tion

of s

ymm

etrie

s in

3D

EXPA

ND

ED

CO

MPA

CT

3

2

3

2

Hea

t sho

ck p

rote

in

Hsp

26

Page 13: Point group symmetries Why is it Important? reveal the

Cub

e 2 4 3

4 2

3

Oct

ahed

ron

Com

bina

tion

of s

ymm

etrie

s in

3D

Com

bina

tion

of s

ymm

etrie

s in

3D

Dod

ecah

edro

n

2 5 3

3

2 5

Icos

ahed

ron

Page 14: Point group symmetries Why is it Important? reveal the

Com

bina

tion

of s

ymm

etrie

s in

3D

http

://w

ww.

staf

f.ncl

.ac.

uk/j.

p.go

ss/s

ymm

etry

/

Icos

ahed

rael

sym

met

ry

Dod

ecah

edro

n Ic

osah

edro

n

3

5

2

3

RC

NM

V- R

ed

clov

er n

ecro

tic

mos

aic

viru

s

Her

pes

viru

s

Te

trahe

dron

Oc

tahe

dron

Cu

be

Dode

cahe

dron

Ico

sahe

dron

Grap

hics

Fa

ces

4 tria

ngles

8 t

riang

les

6 squ

ares

12

pen

tago

ns

20 tr

iangl

es

Verti

ces

4 6

8 20

12

Ed

ges

6 12

12

30

30

Po

int G

roup

Td

Oh

Oh

Ih Ih

For e

ach

of th

e po

int g

roup

s T d

, Oh,

and

I h th

ere

exis

ts s

ub-g

roup

s T,

O, a

nd I

whi

ch c

onta

in a

ll C

n sym

met

ry e

lem

ents

, but

non

e of

the

Sn o

pera

tions

(inc

ludi

ng

inve

rsio

n an

d re

flect

ion)

. Add

ing

a σ h

mirr

or p

lane

or a

n in

vers

ion

cent

er to

the

T gr

oup

yiel

ds T

h.

The

high

-sym

met

ry p

oint

gro

ups

in w

hich

mor

e th

an o

ne C

n axi

s w

ith n

≥ 3

is

pres

ent a

re b

est v

isua

lized

by

the

five

regu

lar p

olyh

edra

(Pla

toni

c so

lids)

as

show

n be

low.

In

thes

e ob

ject

s, a

ll fa

ces,

ver

tices

, and

edg

es a

re s

ymm

etry

re

late

d an

d th

us e

quiv

alen

t. T

he o

ctah

edro

n an

d th

e cu

be a

re c

lose

rela

ted

to

each

oth

er a

s th

ey c

onta

in th

e sa

me

sym

met

ry e

lem

ents

, but

in d

iffer

ent

orie

ntat

ions

. Th

e sa

me

appl

ies

to th

e do

deca

hedr

on a

nd ic

osah

edro

n.

Page 15: Point group symmetries Why is it Important? reveal the

1.Bu

rns,

G.;

Gla

zer,

A. M

. (19

90).

Spa

ce G

roup

s fo

r S

cien

tists

and

Eng

inee

rs (2

nd e

d.).

Bost

on: A

cade

mic

Pr

ess,

Inc.

ISBN

0-1

2-14

5761

-3.

2.C

legg

, W (1

998)

. Cry

stal

Stru

ctur

e D

eter

min

atio

n (O

xfor

d C

hem

istry

Prim

er).

Oxf

ord:

Oxf

ord

Uni

vers

ity

Pres

s. IS

BN 0

-19-

8559

01-1

. 3.

O'K

eeffe

, M.;

Hyd

e, B

. G. (

1996

). C

ryst

al S

truct

ures

; I.

Pat

tern

s an

d S

ymm

etry

. Was

hing

ton,

DC

: Min

eral

ogic

al

Soci

ety

of A

mer

ica,

Mon

ogra

ph S

erie

s. IS

BN 0

-939

950-

40-5

. 4.

Mille

r, W

illard

Jr.

(197

2). S

ymm

etry

Gro

ups

and

Thei

r A

pplic

atio

ns. N

ew Y

ork:

Aca

dem

ic P

ress

. OC

LC 5

8908

1.

Ret

rieve

d 20

09-0

9-28

. 5.

http

://en

.wik

iped

ia.o

rg/w

iki/S

pace

_gro

up

6.ht

tp://

en.w

ikip

edia

.org

/wik

i/Poi

nt_g

roup

Furth

er re

adin

g Sc

hoen

flies

not

atio

n In

Sch

oenf

lies

nota

tion,

poi

nt g

roup

s ar

e de

note

d by

a

lette

r sym

bol w

ith a

sub

scrip

t. Th

e sy

mbo

ls m

ean

the

follo

win

g:

The

lette

r I (f

or ic

osah

edro

n) in

dica

tes

that

the

grou

p ha

s th

e sy

mm

etry

of a

n ic

osah

edro

n.

The

lette

r O (f

or o

ctah

edro

n) in

dica

tes

that

the

grou

p ha

s th

e sy

mm

etry

of a

n oc

tahe

dron

(or c

ube)

. Th

e le

tter T

(for

tetra

hedr

on) i

ndic

ates

that

the

grou

p ha

s th

e sy

mm

etry

of a

tetra

hedr

on.

1.

Loo

k at

the

mol

ecul

e an

d se

e if

it se

ems

to b

e ve

ry

sym

met

ric o

r ver

y un

sym

met

ric. I

f so,

it p

roba

bly

belo

ngs

to

one

of th

e sp

ecia

l gro

ups

(low

sym

met

ry: C

1, C

s, C

i or l

inea

r, C

h, D

h) o

r hig

h sy

mm

etry

(Td,

Oh,

I h).

2. F

or a

ll ot

her m

olec

ules

find

the

rota

tion

axis

with

the

high

est n

, the

hig

hest

ord

er C

n ax

is o

f the

mol

ecul

e.

3. D

oes

the

mol

ecul

e ha

ve a

ny C

2 axe

s pe

rpen

dicu

lar t

o th

e C

n axi

s? If

it d

oes,

ther

e w

ill be

n o

f suc

h C

2 axe

s, a

nd th

e m

olec

ule

is in

one

of D

poi

nt g

roup

s. If

not

, it w

ill be

in o

ne o

f C

or S

poi

nt g

roup

s.

4. D

oes

it ha

ve a

ny m

irror

pla

ne (s

h) p

erpe

ndic

ular

to th

e C

n ax

is. I

f so,

it is

Cnh

or D

nh.

5. D

oes

it ha

ve a

ny m

irror

pla

ne (s

d,sv)?

If s

o, it

is C

nv o

r Dnd