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PigmentWhy does this bacteria change color?
Garcia, AngelaUrbina, Maria
2
Who?
• Angela Garcia (right)• Maria Urbina (left)
• Northeast Magnet High School
• Bio-Research Club 2006-2007
• Juniors
Garcia, AngelaUrbina, Maria
3
Experiments• Experiment # 1 Salinity Concentration
– To what extent does the salt concentration affect marinococcus coloration?
• Experiment # 2 Darkness– To what extent does the light in the room affect
marinococcus coloration?• Experiment # 3 Temperature (°C)
– To what extent does the temperature affect marinococcus coloration?
• Experiment # 4 pH level– To what extent does the pH level affect marinococcus
coloration?
Garcia, AngelaUrbina, Maria
4
Marinococcus• Flavin pigment
– (natural yellow pigment)
• Halophilic – (grows only in salt rich environment)
• Gram positive cocci – (Spherical, spiral, or curved shaped bacteria
that stained purple/blue during iodine staining)
Garcia, AngelaUrbina, Maria
5
Items being tested• Marinococcus
– # 31 (light yellow)– # 32 (bright orange)
• Rate at which Growth can be Achieved – 1% - 20% Salt Content– 4° - 50° C– pH 9 - pH 11
• Control– 37°C– pH 9– 10% Salt Concentration
Garcia, AngelaUrbina, Maria
6
Marinococcus
Our bacteria, here you can see the difference in the colors.
Garcia, AngelaUrbina, Maria
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Ex: 1 Salinity Concentration
• To want extent does salinity concentration affect coloration of marinococcus? – H1 If salt content is increased then the
coloration of marinococcus will be affected.
– H0 If salt content is increased then the coloration of marinococcus will not be affected.
– H2 If salt content is decreased then the coloration of marinococcus will be affected.
Garcia, AngelaUrbina, Maria
8
The Test• Testing
– Minimum 1% salinity – 5% salinity– Optimum 10% salinity– Maximum 20% salinity
• Control– Temperature - 37° C– pH level - 9
• Amount Tested– 24 petri dishes will be set up. 12 for each type, #31 &
#32, of the bacteria.
Garcia, AngelaUrbina, Maria
9
Preliminary Data
• 2-8-07– Started Colonies
on 1%,5%,10%, and 20% agar plates. Also began new colonies and placed the older ones in freezer to prevent further growth.
Garcia, AngelaUrbina, Maria
10
Collecting bacteria from the original slants.
Proper technique for creating individual colonies.
Preparing to flame loop for transfer.
Fun in the lab.
Garcia, AngelaUrbina, Maria
11
First Week Results
• 2-15-07 • #31
– 1% - lead sized individual colonies with diminutive, small, growth
– 5% - lumped colonies in a strip line but no individual growth
• 2-15-07• #32
– 1% - diminutive growth better than the 20%
– 5% - an abundant of tiny colonies sparsely spread
Garcia, AngelaUrbina, Maria
12
• 2-15-07• #31
– 10% - As expected, colonies grew quite well, many useable individual colonies
– 20% - One mass colony, most likely the first streak when transferred, no individual colonies
• 2-15-07• #32
– 10% - As expected, colonies grew quite well, many useable individual colonies
– 20% - Two mass colonies, most likely the first streak when transferred, no individual colonies
First Week Results
Garcia, AngelaUrbina, Maria
13
Other Notes• 2-15-07
– The temperature had settled to 30°C on 2-15-07 so we moved it up to the control 37°C.
– Conclusion after first week• The salinity concentration has no affect on
coloration, just on the growth of marinococcus.
Garcia, AngelaUrbina, Maria
14
Images• 2-21-07
• 2-22-07
Garcia, AngelaUrbina, Maria
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Images• 2-21-07
Garcia, AngelaUrbina, Maria
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Images• 2-21-07
Garcia, AngelaUrbina, Maria
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Images• 2-21-07
Garcia, AngelaUrbina, Maria
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Other Notes• 2-21-07
– Temperature was back at 30°C, moved up to the control
– No useable image of 1% #31 was taken so 2-22-07 another picture will be collected
– Agar has dried out, crystals have formed on most plates
Garcia, AngelaUrbina, Maria
19
Ex : 2 Darkness
• To want extent does the light in the room affect coloration of marinococcus? – H1 If colonies of the marinococcus are kept in
the dark then coloration will be affected.
– H0 If colonies of the marinococcus are kept in the dark then coloration will not be affected.
– H2 If colonies of the marinococcus are kept in the light then coloration will not be affected.
Garcia, AngelaUrbina, Maria
20
The Test• Testing
– Dark (dark box)– Light (Florescent light)
• Control– Salt Concentration - 10%– Temperature - 37° C– pH level - 9
• Amount Tested– 6 petri dishes will be set up. 3 for each type, #31 &
#32, of the bacteria.