8/9/2019 PI480031_NMDA NR2A, N-terminus, Rabbit Polyclonal Antibody Rev 0.0

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NMDA NR2A, N-terminus, RabbitPolyclonal AntibodyCatalog no. 480031(See product label for lot information)

Product DescriptionAffinity purified rabbit polyclonal antibody

Clone/PAD: N/AIsotype: N/AQty: 100l

Formulation10 mM HEPES (pH 7.5), 150 mM NaCl,100 g per ml BSA and 50% glycerol.

Purification Method

Prepared from rabbit serum by affinitypurification using a column to which thepeptide immunogen was coupled.

ValidationSee www.invitrogen.com/antibodiesfor protocols

Validated for use in WB.

WB: 1:1000

ReactivityThis product had been directly tested forreactivity with Rat.

ImmunogenPeptide from the N-terminus of the NR2Asubunit of rat NMDA receptor.

Storage2-8C for up to 1mo, -20C for long termstorage. Avoid repeated freezing andthawing.

Expiration DateExpires one year from date of receipt whenstored as instructed.

BackgroundThe ion channels activated by glutamate are typically divided into twoclasses. Glutamate receptors that are activated by kainate and -amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known askainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). TheNMDAR plays an essential role in memory, neuronal development and it hasalso been implicated in several disorders of the central nervous systemincluding Alzheimers, epilepsy and ischemic neuronal cell death (Grosshanset al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDAreceptor is also one of the principal molecular targets for alcohol in the CNS(Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDARis also potentiated by protein phosphorylation (Lu et al., 1999). The ratNMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1protein can form NMDA activated channels when expressed in Xenopusoocytes but the currents in such channels are much smaller than those seenin situ. Channels with more physiological characteristics are produced whenthe NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D)subunits.

Western blot of

specific immunol

NMDA receptor.

10 ug of rat hippocampal lysate showing

abeling of the ~180k NR2A subunit of the

ReferencesAlvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003

Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor functionJ Biol Chem 278:11020-11025.

Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptictransmission and plasticity. Trends Neurosci 25:571-577.

Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surfaceexpression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.

Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in

hippocampal neurons. Science 243:1721-1724.Lu W-Y, Xiong Z-G, Lei S, Orser BA, Browning MD, MacDonald JF (1999) G-protein coupled

receptors act via protein kinase C and Src to regulate NMDA receptors. Nature Neurosc2:331-338.

Snell LD, Nunley KR, Lickteig RL, Browning MD, Tabakoff B, Hoffman PL (1996) Regional andsubunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brainfollowing chronic ethanol ingestion. Mol Brain Res 40:71-78.

Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDAreceptors. Annu Rev Pharmacol Toxicol 43:335-358.

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