8/9/2019 PI480031_NMDA NR2A, N-terminus, Rabbit Polyclonal
Antibody Rev 0.0
1/1
This product is for research use only. Not for use in diagnostic
procedures.
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NMDA NR2A, N-terminus, RabbitPolyclonal AntibodyCatalog no.
480031(See product label for lot information)
Product DescriptionAffinity purified rabbit polyclonal
antibody
Clone/PAD: N/AIsotype: N/AQty: 100l
Formulation10 mM HEPES (pH 7.5), 150 mM NaCl,100 g per ml BSA
and 50% glycerol.
Purification Method
Prepared from rabbit serum by affinitypurification using a
column to which thepeptide immunogen was coupled.
ValidationSee www.invitrogen.com/antibodiesfor protocols
Validated for use in WB.
WB: 1:1000
ReactivityThis product had been directly tested forreactivity
with Rat.
ImmunogenPeptide from the N-terminus of the NR2Asubunit of rat
NMDA receptor.
Storage2-8C for up to 1mo, -20C for long termstorage. Avoid
repeated freezing andthawing.
Expiration DateExpires one year from date of receipt whenstored
as instructed.
BackgroundThe ion channels activated by glutamate are typically
divided into twoclasses. Glutamate receptors that are activated by
kainate and -amino-3-hydroxy-5-methyl-4-isoxalone propionic acid
(AMPA) are known askainate/AMPA receptors (K/AMPAR). Those that are
sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA
receptors (NMDAR). TheNMDAR plays an essential role in memory,
neuronal development and it hasalso been implicated in several
disorders of the central nervous systemincluding Alzheimers,
epilepsy and ischemic neuronal cell death (Grosshanset al., 2002;
Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDAreceptor
is also one of the principal molecular targets for alcohol in the
CNS(Lovinger et al., 1989; Alvestad et al., 2003; Snell et al.,
1996). The NMDARis also potentiated by protein phosphorylation (Lu
et al., 1999). The ratNMDAR1 (NR1) was the first subunit of the
NMDAR to be cloned. The NR1protein can form NMDA activated channels
when expressed in Xenopusoocytes but the currents in such channels
are much smaller than those seenin situ. Channels with more
physiological characteristics are produced whenthe NR1 subunit is
combined with one or more of the NMDAR2 (NR2 A-D)subunits.
Western blot of
specific immunol
NMDA receptor.
10 ug of rat hippocampal lysate showing
abeling of the ~180k NR2A subunit of the
ReferencesAlvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T,
Yamamoto T, Browning MD (2003
Tyrosine dephosphorylation and ethanol inhibition of
N-methyl-D-aspartate receptor functionJ Biol Chem
278:11020-11025.
Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and
targeting: implications for synaptictransmission and plasticity.
Trends Neurosci 25:571-577.
Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP
leads to rapid surfaceexpression of NMDA but not AMPA receptors in
adult rat CA1. Nat Neurosci 5:27-33.
Lovinger DM, White G, Weight FF (1989) Ethanol inhibits
NMDA-activated ion current in
hippocampal neurons. Science 243:1721-1724.Lu W-Y, Xiong Z-G,
Lei S, Orser BA, Browning MD, MacDonald JF (1999) G-protein
coupled
receptors act via protein kinase C and Src to regulate NMDA
receptors. Nature Neurosc2:331-338.
Snell LD, Nunley KR, Lickteig RL, Browning MD, Tabakoff B,
Hoffman PL (1996) Regional andsubunit specific changes in NMDA
receptor mRNA and immunoreactivity in mouse brainfollowing chronic
ethanol ingestion. Mol Brain Res 40:71-78.
Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS
(2003) Trafficking of NMDAreceptors. Annu Rev Pharmacol Toxicol
43:335-358.
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