PHR 4600 Tuesday Afternoon Lab SectionRat Stomach Fundus (Smooth Muscle Stimulation!ata "om#iled $n "lass on %%&%'&%4()#eriment *%+!ose,res#onse for A"h,mediated smoth muscle contraction (-Final [ACh] (M) Smooth Muscle Contraction (gramsAverage Std Dev n1.00E-08 0.000 0 0 0 0 33.00E-08 0.000 0 0 0 0 31.00E-07 0.000 0 0 0 0 33.00E-07 0.022 0.23 0.33 0.1940.157124 31.00E-06 0.050 0.41 0.64 0.3666670.297377 33.00E-06 0.080 0.52 0.72 0.440.327414 31.00E-05 0.250 0.36 0.92 0.510.359305 33.00E-05 1.050 0.78 1.25 1.0266670.235867 31.00E-04 1.350 1.83 1.97 1.7166670.325167 33.00E-04 2.460 2.47 2.98 2.6366670.297377 31.00E-03 4.560 4.7 4.2 4.4866670.257941 3Final [ACh] (M) Smooth Muscle Contraction (gramsAverage Std Dev n1.00E-08 0 0 0 0 0 33.00E-08 0 0 0 0 0 31.00E-07 0 0 0 0 0 33.00E-07 0.12 0.35 44 14.82333 25.268 31.00E-06 0.95 0.78 0.91 0.880.088882 33.00E-06 0.95 0.88 1.79 1.2066670.506392 31.00E-05 1.63 0.36 2 1.330.860174 33.00E-05 2.25 0.78 2.62 1.8833330.973259 31.00E-04 3.45 2.47 2.95 2.9566670.490034 33.00E-04 4.25 1.83 3.25 3.111.216059 31.00E-03 5.55 3.09 4.3 4.3133331.230054 3Smooth Muscle Contraction (grams) Average Std Dev3.86 5.730 4 3.1 5.5633331.111077()#eriment *.+!ose,res#onse for A"h,mediated smoth muscle contraction (- #retreated /ith % ) %0,6 M TubocurarineExperiment #3:3 x 1!" M #ilocarpineFinal [#ilo] (M) 3 x 10-6 M PilocarpineFinal [ACh] (M) Smooth Muscle Contraction (gramsAverage Std Dev n1.00E-08 0.13 0.1 0.09 0.1066670.020817 33.00E-08 0.29 0.36 0.56 0.4033330.140119 31.00E-07 0.25 0.51 0.65 0.470.202978 33.00E-07 0.8 0.54 0.73 0.690.134536 31.00E-06 1 1.65 1.46 1.370.334215 33.00E-06 1.57 2.42 1.71 1.90.455741 31.00E-05 2.75 3.6 2 2.7833330.800521 33.00E-05 3.83 4.41 2.01 3.4166671.252251 31.00E-04 3.93 4.81 2.56 3.7666671.133858 33.00E-04 4.34 5.13 3.44 4.3033330.845596 31.00E-03 4.51 5.81 4.83 5.050.677348 3Final [#ilo] (M) Smooth Muscle Contraction (gramsAverage Std Dev n2.1 1.2 0.9 1.4 0.6245 3Final [ACh] (M) Smooth Muscle Contraction (gramsAverage Std Dev n1.00E-08 0 0.000 0 0 0 33.00E-08 0 0.000 0.00 0 0 31.00E-07 0 0.000 0.00 0 0 33.00E-07 0 0.000 0.00 0 0 31.00E-06 0.05 0.000 0.00 0.0166670.028868 33.00E-06 0.25 0.600 0.00 0.2833330.301386 31.00E-05 0.75 0.500 0.00 0.4166670.381881 33.00E-05 1.55 2.500 0.00 1.351.261943 31.00E-04 2.35 3.500 2.00 2.616667 0.78475 33.00E-04 4.15 5.300 6.00 5.150.934077 31.00E-03 5.65 6.900 7.70 6.751.033199 3Final [#ilo] (M) Smooth Muscle Contraction (grams) Average Std Dev0 0 0 0.1 0.033333 0.05Experiment #$:%ose!response &or ACh!me'iate' smoth muscle contraction (g) pretreate' (ith 3 x 1!" M ph)sostigmineExperiment #*:3 x 1!" M #ilocarpine pretreare' (ith 3 x 1!" M ph)sostigmine3 x 10-6 M PilocarpineExperiment #":%ose!response &or ACh!me'iate' smoth muscle contraction (g) pretreate' (ith 1 x 1!+ M atropineExperiment #+:3 x 1!" M #ilocarpine pretreare' (ith 1 x 1!+ M atropine3 x 10-6 M Pilocarpine()#eriment *%+!ose,res#onse for A"h,mediated smoth muscle contraction (- 1.00E-083.00E-08Std Error 1.00E-070 3.00E-070 1.00E-060 3.00E-060.090716 1.00E-050.171691 3.00E-050.189033 1.00E-040.207445 3.00E-040.136178 1.00E-030.1877350.1716910.148922Std Error00014.588480.0513160.2923660.4966220.5619110.2829210.7020920.710172n Std Error40.555538Pilocarpine5.563333()#eriment *.+!ose,res#onse for A"h,mediated smoth muscle contraction (- #retreated /ith % ) %0,6 M Tubocurarine 3 x 10-6 M Pilocarpine0.555538Std Error0.0120190.0808980.1171890.0776750.1929590.2631220.4621810.7229880.6546330.4882050.391067Std Error0.360555Std Error00000.0166670.1740050.2204790.7285830.4530760.539290.596518n Std Error4 0.025Experiment #$:%ose!response &or ACh!me'iate' smoth muscle contraction (g) pretreate' (ith 3 x 1!" M ph)sostigmineExperiment #":%ose!response &or ACh!me'iate' smoth muscle contraction (g) pretreate' (ith 1 x 1!+ M atropine0123456Res#onse of Pilocar#ine 0ith 1ther "hemicals"hemical ()#erimented 0ithSmooth Muscle "ontraction (-ramsExp 1 Exp 4 Exp 6 0.1184920.3148120.2480560 0.106667 0 00.012019 00 0.403333 0 00.080898 00 0.47 0 00.117189 00.194 0.69 0 0.0907160.077675 00.366667 1.37 0.016667 0.1716910.1929590.0166670.44 1.9 0.283333 0.1890330.2631220.1740050.51 2.783333 0.416667 0.2074450.4621810.2204791.026667 3.416667 1.35 0.1361780.7229880.7285831.716667 3.766667 2.616667 0.1877350.6546330.4530762.636667 4.303333 5.15 0.1716910.488205 0.539294.486667 5.05 6.75 0.1489220.3910670.596518PilocarpinePilocarpine and atropine1.4 0.0333330.360555 0.0250123456Res#onse of Pilocar#ine 0ith 1ther "hemicals"hemical ()#erimented 0ithSmooth Muscle "ontraction (-rams4.24.254.34.354.44.454.54.55Ma)imum Acetylcholine Res#onse A-ainst Ma)imum Tubucurarine"hemical ()#erimented 0ithSmooth Muscle "ontraction (-ramsMaximum c Maximum !u"ucurarine4.486667 4.3133330.00E#00 2.00E-04 4.00E-04 6.00E-04 8.00E-04 1.00E-03 1.20E-03012345678!ose Res#onse "ur2e of Acetylcholine and "hemicals cet$lc%olinecet$lc%oline and P%$&o&ti'minecet$lc%oline and tropineFinal Acetylcholine "oncentration (MSmooth Muscle "oncentration (-rams4.24.254.34.354.44.454.54.55Ma)imum Acetylcholine Res#onse A-ainst Ma)imum Tubucurarine"hemical ()#erimented 0ithSmooth Muscle "ontraction (-rams0.00E#00 2.00E-04 4.00E-04 6.00E-04 8.00E-04 1.00E-03 1.20E-03012345678!ose Res#onse "ur2e of Acetylcholine and "hemicals cet$lc%olinecet$lc%oline and P%$&o&ti'minecet$lc%oline and tropineFinal Acetylcholine "oncentration (MSmooth Muscle "oncentration (-rams