Phn lp v nhn ging nm - (theo: MUSHROOM CULTIVATION)PRODUCING PDA
MEDIUMNui cy m nm Chn m nui cy Cy chuyn t mi trng PDA sang PDA
Nui cy m nm1. Chun b nguyn liu: Khoai ty: 200 gr. ng Dextrose:
20 gr. Thch Agar: 20 gr. Nc: 1 l.t Bng khng thm nc Ghi ch: - Nu
khng c ng Dextrose th mua ng ma, sacharose thay th cng c. - Thch v
bng mua siu th ch loi Agarose nguyn cht. - Khoai ty chn loi khng b
thi, ...
2. Khoai ty ra sch v ct thnh hnh khi mt cm;
3. chn v ra sch chai l dng (c th dng chai whiskey hoc chai coca,
sa u nnh - thy tinh trng).
4. Cho khoai vo ni, thm nc va mt lt, un si khong 15 n 20
pht;
5. Gn ly dch trong (nc chit khoai ty), thm nc n 1 lt
6. Mang vo bp. thm ng, Agar - khuy u tay vad un nh la cho tan
ht
7. Chia v cc chai, mi chai 5 - 10 mm mi trng.
8. y bng bng khng thm nc.
9. Kh trng 121oC trong 20 - 30 pht. ngui ni n 37oC.
10. t nghing (nh hnh) cc chai mi trng lan u sao cho mi trng khng
chm vo nt bng. Khi mi trng trong chai ng hn, ct vo ni kh ro, sch s
dng dn.
11. mt vi bnh ra ngoi mi trng kim tra. Nu sau mt thi gian thy cc
nt, hoc m trn b mt thch l mi trng cha hoan ton c tit trng
Chn m nm nui cy1. Chun b nguyn liu:
Dao m chuyn dng, kp ... kh trng n cn Cn 70 khng thm nc Bt la Cc
chai c mi trng PDA T cy n UV
2. Chn m nm khe mnh nui cy.
Cy nm khe. Khng qu gi, khng qu non. Khng qu m (t nht 2-3 gi sau
khi ti) Thn cng Khng nhim cc loi nm khc.
3. Lm sch phng v tt c cc dng c cn thit, bn trong v ngoi t cy bng
cn. Chuyn cc chai mi trng PDA, dng c cn thit vo khoang.
4. t tt c dng c v dng cn thit vo khoang, Bt n tia cc tm, qut
thoong gi t cy . Sau 10-15 pht, tt n UV, li qut thng gi sut thi
gian s dng.
5. Ra hai tay bng cn.
6. Gi dao ct bng hai ngn tay, nghing 45o trn ngon la n cn cho n
khi li dao hng ln.
7. Trong khi ch ngui, (15-20 giy - gi cho dao khng chm vo bt k b
mt no - c th cm ngc cn vo cc chun b trc).
8. Dng tay x cy nm theo chiu dc - khng dng dao
9. Dng dao ct mt mu nh (2 mm x 2 mm) m nm - v tr gia m nm v thn
nm. ly gia, chc chn mu sch v khng nhim cc loi vi sinh vt khc
10. H ming hcai quanh ngn la n cn. Dng tay khc m nt bng trc n
cn
11. a mu va ct vo gia b mt mi trng PDA trong chai - khng chm mu
vo thnh chai
12. H ming chai mt ln na v y li nt bng - vn gn ngn la Ch : y
chai lun thp hn ming chai v gn ngn la trong sut qu trnh thao
tc.
13. Dn nhn: Ngy thng, tn loi nm.
Cy chuyn t mi trng PDA sang PDAKhi cy vo mi trng PDA, h si pht
trin khong 15 ngy . (Ty thuc vo loi). 9. Dng dao ct mt mu nh (5 mm
x 5 mm) h si nm trn mi trng PDA . 10. H ming bnh, tho nt bng (ging
hnh).
11. a ming mi trng c cha h si nm vo gia b mt thch trong chai
12. y nt bng gn ng la.
13. Dn nhn, ghi y thng tin nh trn.
14. Thi gian nui cy khong 10 - 15 ngy, ty loi
15. cc chai c s nm pht trin ni kh ro, sch s. Kim tra s pht trin
hng ngy
16. Khi si nm lan kn mi trng, lu gi trong ngn mt t lnh.
17. Kim tra cc chai i changs xc nh nguyn nhn gy nhim, phng cc tc
nhn ny nhng l sau.
18. Ghi chp li y cc thng tin quan st c
Ngun trch dn (0) Tm tag: MUSHROOM CULTIVATION, nhn ging nm, PDA
medium, trng nm, mi trng, nhn ging
