PCR Polymerases...Enzyme properties Enzyme Titanium Taq Advantage 2 Polymerase 5’–3’ exonuclease activity 3'–5' exonuclease activity Yes T/A overhangs or blunt T/A T/A Fidelity

PCR PolymerasesHigh yield. High fidelity. Lyophilized. Long PCR. GC-Rich. Direct PCR. Fast PCR. Standard PCR.

Whether you’re performing next-generation sequencing, cloning, genotyping, or using other technologies, the successful amplification of DNA is key. We understand how critical it is for your PCR polymerases to perform reliably for every single reaction, and that your time and resources are limiting. Each sample and every decision matters—because every result counts.

Your success is what drives our scientists to develop the highest-performing enzymes. Takara Bio has over 90 years of experience in pursuing excellence in biochemistry tools, beginning with the improvement of fermentation techniques for cultivating microbes. In 1950, we launched a biotech R&D center that has yielded cutting-edge advances. This established practice of quality and progress continues today—our extensive portfolio of PCR polymerases supports a wide variety of applications, from basic research to routine laboratory testing, for laboratories around the world. The technical support you receive from Takara Bio comes from decades of experience in fine-tuning enzyme performance.

High performance, value, and expert technical support are the reasons that scientists worldwide have published tens of thousands of peer-reviewed studies using PCR polymerases from Takara Bio. We create polymerases that simply work harder, better, and faster—so you can focus on obtaining the results you need.

DNA Polymerases for PCR success

Powerful enzymes deliver powerful results.Best-in-class enzymes backed by expert scientific support

We create

polymerases that

simply work harder,

better, and faster—

so you can focus

on pushing your

experiments to their

full potential.

Predecessor to Takara Bio begins as a small sake brewery business

Formally incorporates as Takara Shuzo Co., Ltd

Takara Bio founds a biotechnology R&D center

Commences sales of the first domestically produced restriction enzymes in Japan

1842 1925 1950 1979

takarabio.com 3

...whatever your needs.

Need commercial or custom products?We offer royalty-free DNA polymerase formulations, glycerol-free enzymes for lyophilization, OEM and customization services, such as custom packaging and product configurations. Please inquire at [email protected].

Acquires an exclusive distribution right in Japan for a gene amplification system using PCR technology

Clontech Laboratories, Inc. is established

Commences sales of Takara branded PCR products

Acquires Clontech Laboratories, Inc. of the United States

Acquires Rubicon Genomics

19881984 1993 2005 2017

High Yieldfor low-copy

targets

page 4

GC-Richfor NGS

library prep

page 8

Direct PCRfrom tissue

page 9

Fast PCRfor rapid screening

page 10

High Fidelityfor cloning,

mutagenesis

page 5

Lyophilizedfor field testing

page 6

Long PCRfor HLA Typing

page 7

Standard PCRfor PCR assays

page 11

PCR

Additional sizes available. Check product page at takarabio.com for more information.

High-Yield PolymerasesSome PCR applications require amplification of targets present at low copy, or even a single copy. In other situations, samples may be so limiting or precious that PCR failure is simply not an option. When yield is critical, we recommend the following polymerases.

Application Mammalian Genotyping

Library Construction Routine PCR Low Copy Number

and/or Dirty Samples

Recommended product

Titanium® Taq Advantage® 2 EmeraldAmp® MAX HS PCR Master Mix

TaKaRa Ex Taq® DNA Polymerase

Choose for: Sensitive and robust Long PCR and

enhanced fidelity Streamlined

workflow Higher fidelity

and yield

Amplicon size

gDNA <2 kb <6 kb <15 kb <20 kb

Plasmid/lambda <2 kb <18.5 kb NA <30 kb

cDNA <4 kb <8.5 kb NA NA

Enzyme properties

5'–3' exonuclease activity

Yes Yes


Yes Yes Yes

T/A overhangs or blunt

T/A T/A T/A T/A

Format/features

Premix Yes Available

Eco-friendly lyophilized format

Available Available

PCR kit with control template and primers available

Yes Yes

Dye added Yes Available

Hot start Yes Yes Yes Available

P R O D U C T S

Cat. # Product Size

639208 Titanium Taq DNA Polymerase 100 Rxns

639201 Advantage 2 Polymerase Mix 100 Rxns

RR330A EmeraldAmp MAX HS PCR Master Mix 160 Rxns

RR001A TaKaRa Ex Taq DNA Polymerase 250 Units

takarabio.com 5

Additional products and sizes available. Check product page at takarabio.com for more information.

High-Fidelity PolymerasesCloning and expression, sequencing, structure-function studies, and genome variation are a few reasons to reach for a high-fidelity PCR polymerase. But not all high-fidelity enzymes are created equal. Many offered by other manufacturers lack the speed or processivity needed for demanding high-fidelity long PCR, fast PCR, or amplification of GC-rich or repetitive targets. Choose from these polymerases for versatile performance whenever accuracy is required.

Application In-Fusion® Cloning Long PCR, Sequencing,

Cloning Cloning and Expression,

DNA Sequencing

Recommended product CloneAmp™ HiFi PrimeSTAR® GXL DNA Polymerase

PrimeSTAR Max DNA Polymerase

Choose for: In-Fusion Cloning Excellent all-around performance on a

variety of templates Highest fidelity

Amplicon size

gDNA <6 kb (human), <10 kb (E. coli) <30 kb <6 kb

Plasmid/lambda <15 kb <40 kb <15 kb

cDNA <6 kb <13.5 kb <10 kb

Enzyme properties


3'–5' exonuclease activity Yes Yes Yes

T/A overhangs or blunt Blunt Blunt Blunt

Fidelity (sequencing) 29X Taq (GC-rich target) 6.5X Taq (GC-rich target) 29X Taq (GC-rich target)

Speed 5 sec/kb10 sec/kb

(rapid protocol)5 sec/kb

GC-rich targets Up to 73%

Format/features

Premix Yes Yes

Hot start Yes Yes Yes

P R O D U C T S

Cat. # Product Size

639298 CloneAmp HiFi PCR Premix 200 Rxns

R050A PrimeSTAR GXL DNA Polymerase 250 Rxns

R045A PrimeSTAR Max DNA Polymerase 100 Rxns


Lyophilized PolymerasesEcoDry™ polymerases offer a reduced environmental footprint, portability, and room-temperature storage convenience—without sacrificing performance. These “green” polymerases help reduce laboratory waste by providing minimized packaging and a lower carbon footprint, due to room-tem-perature shipping and storage. Choose from EcoDry premixes optimized for high yield or high fidelity.

Application High-Throughput Genotyping High-Throughput Genotyping

Recommended product High Yield PCR EcoDry Premix High Fidelity PCR EcoDry Premix

Choose for: High yield and ease of use Long PCR and enhanced fidelity

Amplicon size

gDNA <2 kb <6 kb

Plasmid/lambda <2 kb <18.5 kb

cDNA <4 kb <8.5 kb

Enzyme properties

Enzyme Titanium Taq Advantage 2 Polymerase

5’–3’ exonuclease activity

3'–5' exonuclease activity Yes

T/A overhangs or blunt T/A T/A

Fidelity (Kunkel) 1X Taq 3X Taq

Format/features

Premix Yes Yes

Eco-friendly lyophilized format Yes Yes

Dye added

Hot start Yes Yes

P R O D U C T S

Cat. # Product Size

639278 High Yield PCR EcoDry Premix 24 Rxns

639282 High Fidelity PCR EcoDry Premix 24 Rxns

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Long PCR PolymerasesWhen your polymerase can go the distance, you can greatly reduce the time and effort needed for your PCR. Instead of amplifying multiple regions across a 30-kb region to cover a gene, you can amplify an entire 30-kb product in one reaction, significantly simplifying your workflow for cloning or screening. Our enzymes are optimized for many needs, from maximum length to repetitive, AT-rich, or GC-rich sequences.

Application Long PCR, Sequencing,

Cloning Long PCR, GC-Rich, Cloning,

Sequencing Long PCR, Sequencing, Cloning

Recommended product TaKaRa LA Taq®

DNA Polymerase TaKaRa LA Taq DNA

Polymerase with GC Buffers PrimeSTAR GXL DNA Polymerase

Choose for: Maximum length High fidelity Excellent all-around

performance on a variety of templates

Amplicon size

gDNA <30 kb <17.5 kb <30 kb


Enzyme properties

5'–3' exonuclease activity Yes Yes


T/A overhangs or blunt T/A T/A Blunt

Fidelity (Kunkel) 6.5X Taq

(Standard %GC target) 6.5X Taq

(Standard %GC target)

Fidelity (sequencing)6.5X Taq

(GC-rich target)

Speed 60 sec/kb 60 sec/kb10 sec/kb

(rapid protocol)

GC-rich targets Up to 67% Up to 74% Up to 73%

Format/features

Premix Available

Hot start Available Yes

P R O D U C T S

Cat. # Product Size

RR002A TaKaRa LA Taq DNA Polymerase (Mg2+ free buffer) 125 Units

RR02AG TaKaRa LA Taq DNA Polymerase with GC Buffer 125 Units

R050A PrimeSTAR GXL DNA Polymerase 250 Units


Polymerases for GC-rich TargetsNot every template has the same %GC content, and many commercially available polymerases fail to amplify GC-rich targets. Not so with these polymerases, which are capable of amplifying even the most recalcitrant GC-rich regions.

Application Robust Genotyping Long PCR, Sequencing, Cloning Amplification Prior to NGS

Recommended product Advantage GC 2 Polymerase Mix

TaKaRa LA Taq DNA Polymerase with GC Buffers

PrimeSTAR GXL DNA Polymerase

Choose for: High %GC Long PCR with GC-rich

templates Highest fidelity

Amplicon size

gDNA <3.5 kb <17.5 kb <30 kb


cDNA <2 kb NA <13.5

Enzyme properties



T/A overhangs or blunt T/A T/A Blunt

Fidelity (Kunkel) 3X Taq

(Standard %GC target) 6.5X Taq

(Standard %GC target)

Fidelity (sequencing) 6.5X Taq (GC-rich target)

Speed 60 sec/kb 60 sec/kb10 sec/kb

(rapid protocol)

GC-rich targets Up to 90% Up to 74% Up to 73%

Format/features

PCR kit with control template and primers

Available Available

Hot start Yes

P R O D U C T S

Cat. # Product Size

639119 Advantage GC 2 PCR Kit 100 Rxns

RR02AG TaKaRa LA Taq DNA Polymerase with GC Buffer 125 Units

R050A PrimeSTAR GXL DNA Polymerase 250 Units

takarabio.com 9


Direct PCR PolymerasesUsing tissue, blood, or cells directly for PCR saves sample handling time by allowing you to skip DNA extraction steps. Free yourself from the effort and cost of purifying DNA prior to PCR analysis: omitting the purification step improves sample turnaround times, resulting in expedited PCR screening conclusions.

Application Mouse Tissues Mouse Tissues Compatible with

Tough Tissue Direct Genotyping on FFPE Samples

Direct Genotyping on FTA Cards

Recommended product

Terra™ PCR Direct Polymerase Mix

Terra PCR Direct Red Dye Premix

Terra PCR Direct Genotyping Kit

Terra PCR Direct FFPE Kit

Terra PCR Direct Card Kit

Choose for: Genotyping Genotyping with

a streamlined workflow

Genotyping with an adaptable

workflow

Genotyping fixed samples

Genotyping archived samples

Amplicon size

gDNA <2 kb <2 kb <2 kb <2 kb <2 kb

Enzyme properties

T/A overhangs or blunt

T/A T/A T/A T/A T/A

GC-rich targets 70% 70% 70% 70% 70%

Format/features

Premix Yes

Dye added Yes Loading dye

included separately

Hot start Yes Yes Yes Yes Yes

Direct PCR Yes Yes Yes Yes Yes

P R O D U C T S

Cat. # Product Size

639270 Terra PCR Direct Polymerase Mix 200 Rxns

639286 Terra PCR Direct Red Dye Premix 200 Rxns

639285 Terra PCR Direct Genotyping Kit 200 Rxns

639284 Terra PCR Direct FFPE Kit 200 Rxns

639287 Terra PCR Direct Card Kit 200 Rxns


Fast PCR PolymerasesThe need for speed in PCR is driven by a sense of urgency. Completing PCR assays in less time means seeing your laboratory results sooner, enabling you to decide quickly on your next step. Our R&D scientists have developed high-speed polymerases that have been used in a range of applications, including innovative assays that are completed in a fraction of the time compared to protocols with conventional reagents.

Application Cloning, Sequencing Colony PCR High-Throughput

Recommended product PrimeSTAR Max DNA Polymerase

SapphireAmp® Fast PCR Master Mix

SpeedSTAR™ HS DNA Polymerase

Choose for: Fast, convenient, and

highest fidelity Fast, routine PCR

Fast, moderate fidelity PCR

Amplicon size

gDNA <6 kb <6 kb <20 kb


cDNA <6 kb

Enzyme properties

5'–3' exonuclease activity Yes Yes


T/A overhangs or blunt Blunt T/A T/A

Speed 5 sec/kb 10 sec/kb 10 sec/kb

Format/features

Premix Yes Yes

Dye added Yes

Hot start Yes Yes Yes

P R O D U C T S

Cat. # Product Size

R045A PrimeSTAR Max DNA Polymerase 100 Rxns

RR350A SapphireAmp Fast PCR Master Mix 160 Rxns

RR070A SpeedSTAR HS DNA Polymerase 250 Units

takarabio.com 11


Standard PCR PolymerasesWhether you’re genotyping samples or performing a PCR-based screening assay, there’s nothing “routine” or “standard” about your reactions. Your progress depends on getting the job done, and having a reliable polymerase is critical. Count on these polymerases for robust performance whether you are running 10 reactions at a time—or 10,000.

Application General Applications Genotyping High Yield Genotyping

Recommended product TaKaRa Taq™ DNA

Polymerase EmeraldAmp GT PCR Master Mix

EmeraldAmp MAX PCR Master Mix

Choose for: Routine use Value choice Streamlined workflow

Amplicon size

gDNA <3 kb <4 kb <15 kb

Plasmid/lambda <12 kb

Enzyme properties

5'–3' exonuclease activity Yes Yes Yes (weak)


T/A overhangs or blunt T/A T/A T/A

Format/features

Premix Available Yes Yes

Dye added Yes Yes

Hot start Available Available

P R O D U C T S

Cat. # Product Size

R001A TaKaRa Taq DNA Polymerase 250 Units

RR310A EmeraldAmp GT PCR Master Mix 160 Rxns

RR320A EmeraldAmp MAX PCR Master Mix 160 Rxns

Expert support and resources facilitate your PCR success.

See how Takara™ and Clontech® polymerases have been used in case studies for translational research:

Long PCR for efficient targeted resequencing

Direct amplification from whole blood samples

Detecting rare alleles with parallel sequencing

takarabio.com/translational-resources

02.17 IN (633712)

Learn more:takarabio.com/pcr

Application Mammalian Genotyping

Library Construction Routine PCR Low Copy Number and/or Dirty Samples

Recommended product Titanium Taq Advantage 2 EmeraldAmp MAX HS PCR Master Mix

TaKaRa Ex Taq DNA Polymerase

Choose for: Sensitive and robust Long PCR and enhanced fi delity

Streamlined workfl ow

Higher fi delity and yield

Amplicon size

gDNA <2 kb <6 kb <15 kb <20 kb

Plasmid/lambda <2 kb <18.5 kb NA <30 kb

cDNA <4 kb <8.5 kb NA NA

Enzyme properties

5'–3' exonuclease activity � �

3'–5' exonuclease activity � � �

T/A overhangs or Blunt T/A T/A T/A T/A

Format/Features

Premix � Available

Eco-friendly lyophilized format

Available Available

PCR kit with control template and primers available � �

Dye added �

Hot start � � � Available

� High Yield

PCR Selection Guide

M 1 2 3 4 5 6 7 8 9 MM 1 2 3 4 5 6 7 8 9 10 M

Human genomic DNA Lambda DNA

Online selection guides

Citation lists

Technical notes

Experienced technical support scientists

Webinars

takarabio.com/pcr

takarabio.com

Takara Bio USA, Inc. United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.565.6999

For Research Use Only. Not for use in diagnostic procedures. © 2017 Takara Bio Inc. All Rights Reserved. All trademarks are the property of Takara Bio Inc. or its affiliate(s) in the U.S. and/or other countries or their respective owners. Certain trademarks may not be registered in all jurisdictions. Additional product, intellectual property, and restricted use information is available at takarabio.com.

Notice to PurchaserOur products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without prior written approval of Takara Bio USA, Inc.

Documents

PCR Polymerases...Enzyme properties Enzyme Titanium Taq Advantage 2 Polymerase 5’–3’ exonuclease activity 3'–5' exonuclease activity Yes T/A overhangs or blunt T/A T/A Fidelity