Comments for pcDNA™3.1D/V5-His-TOPO®

5514 nucleotides

CMV promoter: bases 232-819T7 promoter/priming site: bases 863-882TOPO® recognition site 1: bases 930-934Overhang sequence (complementary strand): bases 935-938TOPO® recognition site 2: bases 939-943V5 epitope: bases 1011-1052Polyhistidine (6xHis) tag: bases 1062-1079BGH reverse priming site: bases 1102-1119BGH polyadenylation signal: bases 1108-1332SV40 early promoter and origin: bases 1833-2142Neomycin resistance gene: bases 2217-3011SV40 early polyadenylation signal: bases 3189-3319pUC origin: bases 3700-4373 (complementary strand)Ampicillin (bla) resistance gene: bases 4518-5378 (complementary strand)bla promoter: bases 5379-5477 (complementary strand)

Hin

d III

Asp

718

IK

pn I

Bam

H I

Eco

R V

Bst

X I

Not

IX

ho I

Xba

IA

pa I

Sac

II

Age

I

Pm

e I

pcDNA™3.1D/V5-His-TOPO®

5514 bp

P C M V

SV40oriA

mp

icillin

BGH pA

Neom

ycin

pUC ori

SV40pA

T7 StopCCC TT

TOPO

GGG AAG TGG

TOPO

AAG GGTTTC CCA V5 epitope 6xHis