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PBG 431 Plant Genetics Recitation Mendelian inheritance. Heart disease, high cholesterol, obesity. Eating barley can help! A principal active ingredient is Beta glucan , a complex carbohydrate found in soluble fiber - PowerPoint PPT Presentation
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PBG 431 Plant Genetics Recitation
Mendelian inheritance
Heart disease, high cholesterol, obesity
• Eating barley can help!• A principal active ingredient is Beta glucan, a
complex carbohydrate found in soluble fiber• Barley has the potential to have higher levels of
beta glucan than any other grain
High beta glucan varieties would be good but….
• Breeding for beta glucan per se is made complicated by quantitative inheritance
• The recessive (waxy starch) allele at the GBSSI locus has a pleiotropic effect - it increases beta glucan
• GBSSI is cloned and therefore the DNA sequence differences between waxy (wx) and non-waxy (Wx) be used as a basis for marker assisted selection
What is the candidate gene for waxy?
What cause the mutation?
Result of the mutation
Glucose
Amylose Amylopectin
Starch
GBSSI
90% amylopectinvs. 75% wild type
Scoring alleles at the Waxy locus
non-waxywaxy
SNP data
Hull-less (naked) varieties could make grain processing
easier……..• Breeding for naked seed is not difficult -
if you are willing to wait for plants to mature
• Nud___ genotypes are hulled; nudnud are hull-less
• The Nud gene has not been cloned - yet!
Hulled and hulless (naked)
Wx and Nud are on the same chromosome
• But they are far enough apart to show independent assortment (no linkage)
Oregon Wolfe Barley Map187 classical loci
BCD14340DsT-667Act8A12RbgMD17MWG837B22scind00046ABC165C25
Bmac039928GBM100730BCD09836GBM104249Bmag021159BG36994064ABC16079
JS10C109
MWG706A119KFP170126
MWG2028150ABC261151KFP257B152WMC1E8160MWG912162ABG387Ascssr04163166
1H
ABG0580scind026221ABG00813scssr1022641scssr0775945GBM106647Pox50scssr0338162Hot168Ebmac0684scssr0223669
scssr0033472ABG35675GBM102377scsnp0334390vrs196Bmag0125102DsT-41105MWG503110GBM1062111KFP203112MWG882A115ABG072133Ebmc0415150cnx1152Zeo1161GBM1019173Aglu5Aglu4176
MWG720179GBM1012185MWG949Ascssr08447193
2H
GBM10740MWG798B6Dst-279BCD70612
alm37Bmac020941ABC32545DsT-6748scssr2569163ABG37765Bmag022573
ABG49998
scsnp00940123
ABG004147
scind02281160MWG883165
HVM62183ABC805191
ABC172202
3H
MWG6340MWG07720HVM4023CDO54229scsnp0164830CDO12231hvknox335ABC303scssr2056941
CDO79544DST-46scind03751scind04312a
50
Tef2scind0172852
GBM102060Bmag035361scind1045568scssr1407980ABG47281GBM105984KFP22193Ebmac070195MWG652B96GBM1048102Hsh113HVM67114KFP241.1117ABG601127
4H
scssr023060MWG6187ABC48312ABG61013
ABG39541scssr0250348NRG045A61scsnp0426062Ale63
ABC30291scind1699194scssr1533496scsnp06144100srh111
RSB001A135scsnp001771420SU-STS1148ABG003B153
Tef3184MWG877188
ABG496203E10757Ascsnp02109224
ABG391228JS10B229ABC622236DsT-33239MWG602A253scssr03906255
5H
Bmac03160
MWG602B36JS10A48GBM102155GBM106867BG29929770HVM3173rob75scssr02093Bmag000976
ABG47487ABG38897scsnp21226103MWG820107
GBM1008134scind04312b142GBM1022145Bmac0040153DsT-32B157DsT-28170DsT-74171MWG798AMWG514173
6H
ABG7040
GBSS-I16AW98258024CDO475MWG08931
ABG38033
scsnp0046072ABC25573ABC165D74scssr1586487GBM103091MWG808DAK64294
scsnp00703103MWG2031104nud108lks2124ABC1024128Bmag0120136DsT-30137WG380B138ABC310B149Ris44151ABC253169ABG461A179WG380ADsT-69184GBM1065191KFP255215ThA1218
7H
• Short awns might be preferable to long awns…
LKs2____ = long awn lks2lks2 = short awn
Nud and LKs2 are on the same chromosome
• They are close enough to show linkage
Oregon Wolfe Barley Map187 classical loci
BCD14340DsT-667Act8A12RbgMD17MWG837B22scind00046ABC165C25
Bmac039928GBM100730BCD09836GBM104249Bmag021159BG36994064ABC16079
JS10C109
MWG706A119KFP170126
MWG2028150ABC261151KFP257B152WMC1E8160MWG912162ABG387Ascssr04163166
1H
ABG0580scind026221ABG00813scssr1022641scssr0775945GBM106647Pox50scssr0338162Hot168Ebmac0684scssr0223669
scssr0033472ABG35675GBM102377scsnp0334390vrs196Bmag0125102DsT-41105MWG503110GBM1062111KFP203112MWG882A115ABG072133Ebmc0415150cnx1152Zeo1161GBM1019173Aglu5Aglu4176
MWG720179GBM1012185MWG949Ascssr08447193
2H
GBM10740MWG798B6Dst-279BCD70612
alm37Bmac020941ABC32545DsT-6748scssr2569163ABG37765Bmag022573
ABG49998
scsnp00940123
ABG004147
scind02281160MWG883165
HVM62183ABC805191
ABC172202
3H
MWG6340MWG07720HVM4023CDO54229scsnp0164830CDO12231hvknox335ABC303scssr2056941
CDO79544DST-46scind03751scind04312a
50
Tef2scind0172852
GBM102060Bmag035361scind1045568scssr1407980ABG47281GBM105984KFP22193Ebmac070195MWG652B96GBM1048102Hsh113HVM67114KFP241.1117ABG601127
4H
scssr023060MWG6187ABC48312ABG61013
ABG39541scssr0250348NRG045A61scsnp0426062Ale63
ABC30291scind1699194scssr1533496scsnp06144100srh111
RSB001A135scsnp001771420SU-STS1148ABG003B153
Tef3184MWG877188
ABG496203E10757Ascsnp02109224
ABG391228JS10B229ABC622236DsT-33239MWG602A253scssr03906255
5H
Bmac03160
MWG602B36JS10A48GBM102155GBM106867BG29929770HVM3173rob75scssr02093Bmag000976
ABG47487ABG38897scsnp21226103MWG820107
GBM1008134scind04312b142GBM1022145Bmac0040153DsT-32B157DsT-28170DsT-74171MWG798AMWG514173
6H
ABG7040
GBSS-I16AW98258024CDO475MWG08931
ABG38033
scsnp0046072ABC25573ABC165D74scssr1586487GBM103091MWG808DAK64294
scsnp00703103MWG2031104nud108lks2124ABC1024128Bmag0120136DsT-30137WG380B138ABC310B149Ris44151ABC253169ABG461A179WG380ADsT-69184GBM1065191KFP255215ThA1218
7H
Wx vs. wx and Nud vs. nud data are from a doubled haploid
population
The expected segregation ratio is……..
If it were a backcross?An F2?
Phenotype #Observed #Expected O - E (O - E)2/E
Total 82 82
Let’s get some practical experience in segregation
analysis!!!!