Pathology Visions Conference

Oil Scanning

Amsterdam, Netherlands

Tuesday, December 4, 2007

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Pathology Visions: Solutions Gallery

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VIEWMANAGE

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4INNOVATION

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Primary Oil Immersions Applications

• Hematopathology, Microbiology, Cytology Applications• Blood Smears, Bone Marrow Smears, Gram Stains, ...

• Oil Immersion Digital Slide Scanning

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Oil Immersion ApplicationsBlood films:

• Representative areas in blood films can be scanned for full diagnostic workup.

• Resolution is sufficient to rule out abnormalities in:

• White blood cells

• Appreciate chromatin structure and identify nucleoli for accurate blast counts

• Distinguish all white cell precursors for an accurate differential count

• Red blood cells

• Spectrum of morphological changes including :

• Hypochromia / blisters, bites, acanthocytes, echinocytes, sickle cells and other schistocyte forms

• Inclusions down to the size of 1 to 2 microns such as Howell-Jolly, Pappenheimer bodies & malarial parasites

• Platelets

Oil ImmersionScanning

EXAMPLE: Hypochromia in red cells: http://www.objectivepathology.com/Public/CBC/Fe%20def.html

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Oil Immersion ApplicationsMicrobiology

• Infectious organisms down to the one or two micron level for : • speciation of malarial parasites in red cells, • identifying Leishmania spp. in histocytes from tissue sections, fungal forms such as Aspergillosis and

Candida.

• EXAMPLE: Leishmania amastigotes in tissue sections:

• http://www.objectivepathology.com/Public/Leishmaniasis/TK%20B07-904%20Leish%20100x.html

Cytology:

• Powerful technique for capturing the fine cytological details in fine needle aspiration cytology.

• For example the fine nuclear features of papillary carcinoma of the thyroid such as the powdery chromatin, intranuclear pseudo-inclusions, micronucleoli and linear nuclear ridges ("grooves").

• EXAMPLE: Papillary carcinoma of the thyroid:

• http://www.objectivepathology.com/PublicClientImages/CAP/Aug-2007/PAP.html

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Pathology ExamplesHypochromia in red cells Leishmania amastigotes in tissue sections:

•Papillary carcinoma of the thyroid:

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Oil Immersion ScanningProduct Offerings

• Products & Features

• ScanScope OS• Dedicated Oil Immersions Digital Slide Scanner• Scans at 100x

• ScanScope® (CS, GL, GL-E) with Oil Immersion• Upgrade installed ScanScopes with Oil Immersion Option• Add Oil Immersion to New ScanScope Orders• Scans at 20x Dry, 40x Dry, 83x with Oil Immersion

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Product OfferingsOil Scanner Product Portfolio

Product Offerings:

1. Scanning service (ScanScope OS)• http://aperio.com/productsservices/serv-scanning.asp

2. Dedicated oil scanner (ScanScope OS)

3. Oil upgrades to existing (ScanScope GL, GL-E, and CS)

4. Oil options for new (ScanScope GL, GL-E, and CS)

Note: No XT Oil Upgrades or Options

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Product Specs ScanScope OS• 100x optical magnification realized

with 1.35 NA Plan Apo oil emersion objective

• Resolution 0.14 μm/pixel resolution

• Single-slide tray

• 5x5mm region in ~5 min (est.)

• 10x10mm region in ~20 min (est.) Maximum scan area recommended

• Height 12.5” x 19” x 20.5” (318 x 465 x502 mm)

• Weight 55 lbs (25 kg)

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Integrated Color Management (ICM) “Aperio systems’ leading image quality is assured”

Glass Slide

ImageScopeColor

TransformNeutral Color Profile

Digital Slide Image Color Corrected for

ScanScope & Monitorcharacteristics

ScanScope

“HP2335.icc” file

sRGB (default)or

Monitor Color Profile(If available)

Default Color Profile(used if no Monitor Color Profile specified)

Digital Slide Image

ScanScope ICC Profile Digital Slide Image (.svs) file with embedded ScanScope Profile

ScanScope Color Profile

Digital Slide Image

• True Color For Lab Calibration Issues• Consistency Inter ScanScope & Inter Monitor• Image Analysis Advantages Eye / Algorithm Consistency

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Integrated Color Management “OFF”

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Integrated Color Management “ON”

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Integrated Color Management “ON” on Left “OFF” on Right

Click to Toggle Integrated Color Management “ON” or “OFF”

VIEWDisplayed if Integrated Color Management is “ON”

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“BigTIFF” Support – Digital Slides Larger Than 4GB “Aperio delivers leading technology based on open standards”

“Aperio systems support the highest resolution

image infrastructure in the industry”

• Until today, we supported images up to 4GB in size

• Now, we will be able to support images up to 1EB (Exabyte)

Today

Now in 9.0

1,000 Times LargerThan Before

1,048,576 Terabytes

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Note: 1. See it for your self at http://www.bigtiff.org 2. Only Oil Scans are typically larger than 4GB

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Spectrum™ Plus with Reporting“Aperio systems deliver Workflow Solutions”

• IHC Report• Breast Panel• Standard Template Supports

• Stains: H&E, HER2, ER, PR• Specimens: Up to 3 per case/project• Slides: Up to 4 per specimen

• Standard Templates Included: IHC, Research Project, Slide List

• Professional Services Available: • Customization of Static & Dynamic (DB) Content & Format

• FAQs:• How can I use Aperio Reporting Today?• What’s Included? What’s Not Included?• Compatible with current dictation workflow?• How do customers get it?

• Who implements it & How? Time? Cost?

• Workflow: Scan, View, Manage, Analyze, & Report

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Marketing Collateral ScanScope® OS

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Marketing Collateral ScanScope® with Oil Immersion

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Marketing Collateral Spectrum™ Plus with Reporting

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Aperio Website Updates

• Affected Web Pages

• ScanScope Systems Web Page

• Data Sheets in Quick Links - Right Nav

• ScanScope® OS

• ScanScope® with Oil Immersion

• Spectrum Web Page

• Data Sheets in Quick Links - Right Nav

• Spectrum™ Plus with Reporting

• Image Analysis Web Page

• Colocalization Algorithm Image & Info

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Global Availability

U.S. & International Distributors and Dealers

• Launch Announced: October 24, 2007

• First Customer Shipment: December 10, 2007

U.S. & International Distributors and Dealers

• Launch Announced: October 24, 2007

• First Customer Shipment: December 10, 2007

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• The Aperio Release 9.0 is an

Exciting Opportunity for all of us!

• ScanScope OS & ScanScope with Oil Immersion• 1st Oil Immersion Scanning Solution

• Spectrum Plus with Reporting • Digital Slide Scanning, Viewing, Management, and Analysis Solution

• Integrated Color Management – True Color Quality throughout

• … And much, much, more…

• Release 9.0 Offers Significant Improvements and New Innovations !

Conclusion

Thank You.

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Holland