BIOLOGICAL SEED INOCULATIONwith unique, innovative

technology for maize, ears of grain, sunflowers, soya and other legumes

powered by:AGROsolution GmbH & CO. KGwww.agrosolution.eu

BIO

patent pending

Bacterial spores spores forming highly tolerant to UV and

solar radiation, heat & cold stress are tolerant to copper,

zinc and sulphur long shelf-life board application range “Shielded Knight”

Phases 0 - 1In Phases 0 to 1 the cells divide into unequal parts within the plant cell.

Phase 2The division and partition in Phase 2 produce what is known as the pre-spore, which moves freely within the cytoplasm of the mother cell.

Phase 3In Phase 3 the peptidoglycan between the two membranes is absorbed, which forms the cortex of the spore.

Phase 4In Phase 4 the spore continues to rip-en by means of hydration and forms a loose, cross-linked spore case.

Phase 5In the fifth and last phase the mother cell autolyses, which releases the thick-walled, ripe spores.

spore wall

germ cell wall

inner membrane

outer membrane

corecortex

spores

sporogenic bacteria

Formation

vegetative cell spore wall

mother cell

free endospore

development of the spore wall

2

5

0 1 3

4

Bacterial cells functional, vegetative highly sensitive to UV and

solar radiation, heat, cold perish in high concentrations

or copper, zinc and sulphur short shelf-life weak application range “Nude Man”

Bacteria vs Spore forming Bacteria

Based on the positive characteristics of the new cultivar of our strains working in wide application range, as compared to conventional bacteria.Due to their high tolerance to heat and cold, the spore forming bacteria start acting even at low temperatures and continue to work even in high temperatures.

Efficiency factor

pili/fimbriae

podcell wall plasma membrane

cytoplasm

ribosomes

flagellanucleoid

(helical DNA)

+ 2 °C + 20 °C

Activ

ity o

f the

pro

duct

s

Temperature

NovaFerm spore forming bacteria

normal bacteria

Rhizo.ABiological seed inoculation with unique, innovative technology for soya and other legumes

BIO

*Bradyrhizobium japonicum (Bj)**

Bj is responsible for forming the root nodules of legumes. Our Bradyrhizobium isolate is an extremophile strain. In our product the used bacterial strains show high tolerance level against the attack of different bacteriophage of rhizosphere to compensate the negative effect of bacteriophage infections. In addition, the innoculant well tolerate the elevated temperature, and cannot loss the activity in presence of heavy metals (i.e. starter fertilizers, comlex microelement minerals, etc).

*Rhizobium meliloti (Rm)**

Rm reduces the negative impact of the dangerous bacteriophages in Rhizobia.

*Bacillus licheniformis (Bl)

Bl is fungistatic bacteria, which grows parallel with the plant and protects it from seeding. Bl prevents the infection of mykotoxin, producing funghi.

WIDE RANGE RESISTANCEAGAINST RHIZOPHAGUS

Bacterial strains*Bradyrhizobium japonicumRhizobium melilotiBacillus licheniformis

** In case of other Legumen cultures, the role of virustrapping controvers depend on the other node formuling bacilli strain as Bradyrhizobium japonicum instead of Rhizobium meliloti.

Application / application rate

Soya: 2 litres Rhizo A per ton seed

All other legumes: 2 - 5 litres Rhizo A per ton seed, depend on the calibration of the seed(alfalfa, pea, beans)

gluten 150 g sugar/molasses per ton seed

Rhizo A can be used with all conventional inoculation processes.

The new spore forming bacteria, developed over 15 years, satisfy all the requirements

of highly effective seed inoculation.

2 l Rhizo A per ton seed conventional inoculant

Extremophile, N-fixing, associative bacteria. Can be coated on ears of grain and maize seed. With wide rangefungistatic effects.

Rhizo.BBiological seed inoculation with unique, innovative technology for maize, ears of grain and sunflowers

BIO

*Azospirillum lipoferum (Al)

Al fixes nitrogen from the air and converts the N2 into Ammonium NH4+ and stores this in a relatively large molecule. This means that the nitro-gen will not wash out. In our product the used bacterial strains show high tolerance level against the attack of different bacteriophage of rhizosphere to compensate the negative effect of bacteriophage infections. In addition, the innoculant well tolerate the elevated temperature, and cannot loss the activity in presence of heavy metals (i.e. starter fertilizers, comlex microelement minerals, etc).

*Bacillus licheniformis (Bl)

Bl is fungistatic bacteria, which grows parallel with the plant and protects it from seeding. Bl prevents the infection of mykotoxin, producing funghi.

Bacterial strains*Azospirillum lipoferumBacillus licheniformis

WIDE RANGE RESISTANCEAGAINST RHIZOPHAGUS

Application / application rate

5 Litres Rhizo B per ton seed

gluten 500 g sugar/ molasses per ton seed

Rhizo B can be used with all conventional inoculation processes.

The new spore forming bacteria, developed over 15 years, satisfy all the requirements

of highly effective seed inoculation.

without inoculant conventional inoculant 5 l Rhizo B per ton seed

Growth of Azospirillum by UV radiation

Growth of B. licheniformis by UV radiation

1,00E+06

0t (h) 6 3012 3618 42 54 60 66 7224 48 0t (h) 6 3012 3618 42 54 60 66 7224 48

cell number NS-AL-01 cell number AL B01937

1,00E+04

1,00E+02

1,00E+00

72; 1590000

72; 3000

1,00E+08

cell number NS-BL-01 cell number BL14580

1,00E+06

1,00E+02

1,00E+04

1,00E+00

72; 45500000

72; 11500

AGROsolution GmbH & CO. KGPrinz-Eugen-Straße 23, 4020 Linz, Austria

T: +43 732 77 43 66-0, F: +43 732 77 43 66-4office@agrosolution.eu, www.agrosolution.eu