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Using the MicroscopeRules for Using the

Microscope1. Use two hands to

carry a microscope,one hand holding thearm, the other holdingthe base.

2. Only use lens paperand water to clean thelenses.

Parts of theMicroscope

eyepieces

bodyrevolvingnosepiecemechanical stage

light source

base

Parts of theMicroscope

fine focusadjustment knob

arm

coarse focusadjustment knob

Parts of theMicroscope

light switch

mechanical stagepositioning knobs

objective lenseson revolvingnosepiece

binocular headwith eyepieces

Binocular Headinterpupillary scale window

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Setting theCalibrated

Diopter Ring

diopter ring

set line

Revolving Nosepiece

Objectives

Scanning Objective: 4X

Low Power Objective: 10X

High Power Objective: 40X

Oil Immersion Objective: 100X

Rules for Using theMicroscope1. Always start off with

the lowest magnificationobjective and work upto higher power oneobjective at a time.

2. Always observe theobjective as it is rotatedinto place to ensure itdoesn’t crash into the slide.

Mechanical Stagemicroscope slide

Rules for Using theMicroscope1. Lower the stage to its

lowest point beforeplacing a slide on thestage.

2. Be sure the scanningobjective is in place.

3. Raise the stage to itshighest point withoutcrashing the slide intothe objective.

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Rules for Using theMicroscope4. Focus initially by

lowering the stageusing coarse focusadjustment knob.

5. After getting a closefocus with the coarsefocus adjustmentknob, improve it usingthe fine focusadjustment knob.

Substage Condenseriris

diaphragmlever

condenseradjustment

Rules for Putting Away theMicroscope1. Rotate scanning

objective into place.2. Lower the stage to its

lowest point.3. Clean lenses, stage,

body, and base ifneeded.

4. Replace protectivecover overmicroscope.

Lab ActivitiesGo to the Virtual Microscopewebsite and view theinstructional video online.

URL:http://www.udel.edu/Biology/ketcham/microscope/

Lab ActivitiesMake observations using thesimulated slides at the VirtualMicroscope website. Drawlabeled diagrams of vieweditems as described in theassignment.

Lab Activities

Complete the “try this” activities(see assignment for details).

Complete other tasks asdescribed in the assignment.