Pacific Northwest Pacific Northwest Ballast Water Research FacilityBallast Water Research Facility

Pacific Northwest Pacific Northwest Ballast Water Research FacilityBallast Water Research Facility

Andrea Copping

Marine Research OperationsPacific Northwest National Laboratory

Sequim, WADecember 2007

Today….Today….Today….Today….

PNW Ballast Water RDTE

Location at PNNL Marine Sciences Lab

Virtual tour of PNNL Sequim Labs

Explain PNNL to you

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RDTE Facility Pacific Northwest Ballast Water Treatment Facility

Pacific Northwest National Laboratory, Sequim WA

Sponsored by National Oceanic and Atmospheric Administrationand United States Fish and Wildlife Service

Northwest RDTE at PNNL SequimNorthwest RDTE at PNNL SequimNorthwest RDTE at PNNL SequimNorthwest RDTE at PNNL Sequim

$1.25M Award by NOAA to build RDTEDesign and construction: October ‘07-January ‘09Only commercial test bed on west coast; one of 2 in countryNRL Key West doing development workCapabilities for testing at Sequim (ecotox, species ecology, chemistry, etc.)

Rationale for Developing Rationale for Developing RDTE Ballast Water RDTE Ballast Water

Facility at SequimFacility at Sequim

Rationale for Developing Rationale for Developing RDTE Ballast Water RDTE Ballast Water

Facility at SequimFacility at Sequim

High quality abundant seawater and freshwater

Reliable permitted wastewater treatment system

Adequate land to add facilities

Established facilities management and O&M

Secure facility

Scientific and engineering staff, experience in protocol development, testing, ballast water testing

Marine Research Operations at SequimMarine Research Operations at SequimMarine Research Operations at SequimMarine Research Operations at Sequim

1965 - Battelle purchased Bugge Clam Cannery site

1973 - Beach wet laboratory & offices

1982 - Uplands analytical chemistry labs and offices

1992 - Twenty new offices at beach

2004 - New 44 office building completed

2006 - New Biotechnology Laboratory

19731965 1982

Today Tomorrow

Protecting people & the environment

Coastal assessment & restoration

Marine biotechnology/ecotoxicology

Coastal Security

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C a n a d a

W a s h i n g t o n

O r e g o n

o

1 6

1 5

1 4

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1 7

1 1

1 6

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1 1S e a S u r f a c e T e m p e r a t u r e

PNNL’s MRO Focused in 3 AreasPNNL’s MRO Focused in 3 AreasPNNL’s MRO Focused in 3 AreasPNNL’s MRO Focused in 3 Areas

Developing new products & energy sources

Securing our ports, coastlines

& waterways

Virtual Tour of the MROVirtual Tour of the MROVirtual Tour of the MROVirtual Tour of the MRO

Sea Water IntakeSea Water IntakeSea Water IntakeSea Water Intake

Two independent intakes

• Class AA seawater from Sequim Bay

Four separate distribution systems

• Seawater and freshwater to testing facilities

Ambient seawater (9°C to 11°C) at a rate of 250 gpm

Seawater supplied to labs at 4°C to 38°C

Up to 20 gpm freshwater from uncontaminated groundwater from 11°C to 30°C

Waste Water Treatment FacilityWaste Water Treatment FacilityWaste Water Treatment FacilityWaste Water Treatment Facility

40,000 gal ground holding tank comprised of four separate cells

Removal of particulates -sedimentation and filtration

Disinfection of biologic agents - breakdown of organics using ozonation

Removal of organics and disinfection byproducts via granular activated carbon adsorption

Treated Wastewater Discharge – permitted through the NPDES process.

Aquatic Wet Laboratory FacilitiesAquatic Wet Laboratory FacilitiesAquatic Wet Laboratory FacilitiesAquatic Wet Laboratory FacilitiesTwo wet laboratory facilities

~5300 sq ft Marine, estuarine, and freshwater bioassay testing

Over 40 species commonly tested

Tiered approach to environmental characterization

Natural resource damage assessment (NRDA)

Remedial investigation / feasibility studies

Risk assessment

Good laboratory practice (GLP) testing

Analytical and Biotech SupportAnalytical and Biotech SupportAnalytical and Biotech SupportAnalytical and Biotech Support

BSL 1 and BSL 2 Laboratories

Flow Cytometer & Microscope (FlowCAM)

• continuously monitors of plankton abundance

Pulsed Amplitude Modulation (PAM) chlorophyll fluorometer

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)

• detection of large, intact biomolecules

Digital imaging microscope

Marine / Estuarine ChemistryMarine / Estuarine ChemistryMarine / Estuarine ChemistryMarine / Estuarine Chemistry

Understanding the source and fate of chemicals in aquatic systems

Standard Water Quality ParametersMetals

Ultra-Low (part-per-trillion) Detection Limits in Seawater Arsenic Speciation & Methylmercury in Water, Sediment,

and Tissue

Organics Fuel Fingerprinting Semipermeable Membrane Devices Endocrine Disrupting Chemicals

Methods Development

Sequim Marine Research Operations Sequim Marine Research Operations Sequim Marine Research Operations Sequim Marine Research Operations

Operating since 1966

Located on 140 acres of uplands and tidelands at mouth of Sequim Bay, Strait of Juan de Fuca, 90 miles from ocean 11,000 sq ft of office space 6000 sq. ft of analytical and general purpose laboratories 1500 sq ft biotechnology laboratory 7700 sq. ft. of wet/bioassay and support laboratory space

Uplands facility ten analytical laboratories and 35 offices

Beach area is comprised of four principal buildings office building with 44 offices, a bioassay laboratory building

two climate-controlled bioassay/culturing labs two isolation rooms and supporting analytical labs

biotechnology laboratory seawater pumping and wastewater treatment facilities

HOW WILL TREATMENT TECHNOLOGIES BE EVALUATED?

Project Example EPA’s Endocrine Disruptor Screening

Program $34 M research program Development of tools for screening &

testing chemicals that have potential endocrine disruptor effects

No standardized tests for evaluating these compounds exist

Final product: EPA-certified protocol

Protocol /Method Development, Evaluation, Standardization and Validation

Expert Staff to meet the needs of the RDTEExpert Staff to meet the needs of the RDTEExpert Staff to meet the needs of the RDTEExpert Staff to meet the needs of the RDTE

The MRO has a total of 85 scientists and engineers in:

•biology•chemistry•physical oceanography •biological oceanography•ecological sciences•environmental sciences•ecotoxicology

•fisheries •marine invertebrate •carcinologist•coastal engineering•chemical engineering •civil engineering•marine engineering•electrical engineering

Team Partners

PNNL in the NorthwestPNNL in the NorthwestPNNL in the NorthwestPNNL in the Northwest

SeattleSeattleSequimSequim

RichlandRichland

Portland, ORPortland, OR

Battelle operates PNNL for the US Department of Energy

Battelle’s purposes stem from the 1925 will Battelle’s purposes stem from the 1925 will of steel industrialist Gordon Battelleof steel industrialist Gordon Battelle

Battelle’s purposes stem from the 1925 will Battelle’s purposes stem from the 1925 will of steel industrialist Gordon Battelleof steel industrialist Gordon Battelle

Non-profit charitable trust – began operations in 192975 years of scientific research and developmentCreative activities of a scientific nature

Application and licensing of inventions, discoveries, and developmentsMajor business is R&D, laboratory operations, and commercial ventures$2.9 billion in annual R&D19,000 employees worldwide

Continuous Operation of PNNL Since 1965Continuous Operation of PNNL Since 1965Continuous Operation of PNNL Since 1965Continuous Operation of PNNL Since 1965

• Battelle won the contract in 1964 and began operating PNL in 1965

• Original mission focused on nuclear technology

FY05 PNNL Business FactsFY05 PNNL Business Facts

Approximately $726 million in business volume

4,200 staff

$288 million in purchases for goods and services

Battelle Now Manages or Battelle Now Manages or Co-Manages 5 National LabsCo-Manages 5 National LabsBattelle Now Manages or Battelle Now Manages or Co-Manages 5 National LabsCo-Manages 5 National Labs

National RenewableEnergy Laboratory (with MRI)

Brookhaven National Laboratory (with SUNY– Stony Brook)

Oak Ridge National Laboratory (with the University of Tennessee)

More than $2 billion

15,000 staff

Idaho National Laboratory

Pacific Northwest National LaboratoryRichland and Sequim, Washington

Thank you for opportunity to be hereThank you for opportunity to be hereThank you for opportunity to be hereThank you for opportunity to be here

We would like to invite you to visit us at Sequim MRO

Andrea Coppingandrea.copping@pnl.gov