P/ACE MDQ plus Capillary Electrophoresis System Site Planning

P/ACE™ MDQ plus Capillary Electrophoresis System

Site Planning Guide

B54950AAFebruary 2015

AB Sciex Pte. Ltd and its affiliates disclaims all warranties with respect to this document, expressed or implied, including but not limited to those of merchantability or fitness for a particular purpose. In no event shall AB Sciex Pte. Ltd. and its affiliates be liable, whether in contract, tort, warranty, or under any statute or on any other basis for special, incidental, indirect, punitive, multiple or consequential damages in connection with or arising from this document, including but not limited to the use thereof.

For research use only. Not for use in diagnostic procedures.

The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners.

AB SCIEX™ is being used under license and SCIEX Separations is a part of AB Sciex.

© 2015 AB Sciex

Find us on the World Wide Web at www.sciex.com.

AB Sciex500 Old Connecticut PathFramingham, MA 01701USA

P/ACE™ MDQ plus Capillary Electrophoresis System Site Planning Guide

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http://www.sciex.com

Contents

Chapter 1 Safety, Notices and Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

General Safety Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5Alerts for Warning, Caution, Important, and Note . . . . . . . . . . . . . . . . . . . . . . . . . 6Instrument Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6Chemical and Biological Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8Moving Parts or Sharp Objects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8Disposal of Devices Containing Mercury Components . . . . . . . . . . . . . . . . . . . . . 9Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9Laser Safety (for Optional Laser Device). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10Safety Symbols and Labels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

High Voltage Electric Shock Risk Symbol . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10Attention Safety Symbol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10Sharp Object Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Laser Light (for Optional Laser Device). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Protective Earth or Ground Terminal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Fuse Warning Symbol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11Off Position of Convenience Switch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11On Position of Convenience Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12For Research Use Only . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

RoHS Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12China RoHS Caution Label. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12China RoHS Environmental Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Other Instrument Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13Recycling Label (WEEE) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13CE Mark Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13RCM Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14CSA Mark Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Chapter 2 Pre-Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15About This Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15Preparation Check List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15Pre-Installation Check List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16Computer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16Detectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Chapter 3 Site Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Site Planning Guide P/ACE™ MDQ plus Capillary Electrophoresis System

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Physical Layout Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19Recommended Bench Space . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20Ventilation Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20Access to Mains Supply Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20Other Environmental Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21

Electrical Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21

Chapter 4 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23P/ACE MDQ™ plus Instrument Specifications . . . . . . . . . . . . . . . . . . . . . . . . . .23

Sample Temperature Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24Capillary Temperature Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24Pressure and Vacuum System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24

Computer Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25Detector Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25

UV Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25Photo Diode Array (PDA) Detector (Optional) . . . . . . . . . . . . . . . . . . . . . . . . .26Laser Induced Fluorescence (LIF) Detector (Optional). . . . . . . . . . . . . . . . . . .26

Chapter 5 Training . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27Pre-Training Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27Additional Training . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27

Revision History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29


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CHAPTER 1

Safety, Notices and Labels

Overview

A description of the symbols and labels that are used on the SCIEX P/ACE™ MDQ plus Capillary Electrophoresis System, or that are shown in this guide, can be found in this section.

Do not attempt to perform any procedure before carefully reading all instructions. If in doubt as to how to proceed in any situation, contact your Sciex representative.

General Safety Information

To prevent personal injury or system damage, read, understand, and obey all safety precautions, warnings in this guide, and labels on the instrument. These labels are shown with internationally recognized symbols. Failure to heed these warnings could result in serious injury. This safety information is intended to supplement federal, state, provincial, and local environmental health and safety (EHS) regulations. The information provided covers system-related safety with regard to the operation of the instrument. It does not cover every safety procedure that should be practised. Ultimately, the user and the organization are responsible for compliance with federal, state, provincial, and local EHS regulations and for maintaining a safe laboratory environment.

For more information, refer to the appropriate laboratory reference material and standard operating procedures.

Sciex urges its customers and employees to comply with all national health and safety standards such as the use of barrier protection. This may include, but is not limited to, protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this or any other automated laboratory instrumentation.

WARNING

If the equipment is used in a manner not specified by Sciex, the protection provided by the equipment may be impaired.


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Alerts for Warning, Caution, Important, and Note

All warnings and cautions in this guide include an exclamation point, framed within a triangle.

The exclamation point symbol is an international symbol which serves as a reminder that all safety instructions should be read and understood before installation, use, maintenance, and servicing are attempted.

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury. It may also be used to indicate the possibility of erroneous data that could result in an incorrect determination. May be used to indicate the possibility of severe instrument damage.

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices. May be used to indicate the possibility of erroneous data that could result in an incorrect determination.

IMPORTANT Used for comments that add value to the step or procedure being performed. Following the advice in the Important adds benefit to the performance of a piece of equipment or to a process.

NOTE Used to call atttention to noteable information that should be followed during installation, use, or servicing of this equipment.

Instrument Safety Precautions

WARNING

Risk of operator injury if:

• All doors, covers and panels are not closed and secured in place prior to and during instrument operation.

• The integrity of safety interlocks and sensors is compromised.

• You contact moving parts.

• You mishandle broken parts.

• Doors, covers and panels are not opened, closed, removed and/or replaced with care.

• Improper tools are used for troubleshooting.

To avoid injury:


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• Keep doors, covers and panels closed and secured in place while the instrument is in use.

• Take full advantage of the safety features of the instrument. Do not defeat safety interlocks and sensors.

• Acknowledge and act upon instrument alarms and error messages.

• Keep away from moving parts.

• Report any broken parts to your Sciex representative.

• Use the proper tools when troubleshooting.

CAUTION

System integrity could be compromised and operational failures could occur if:

• This equipment is used in a manner other than specified. Operate the instrument as instructed in the system documentation.

• You introduce software that is not authorized by Sciex into your computer. Only operate your system’s computer with software authorized by Sciex.

• You install software that is not an original copyrighted version. Only use software that is an original copyrighted version to prevent virus contamination.

CAUTION

If you purchased this product from anyone other than Sciex or an authorized Sciex distributor, and, if it is not presently under a Sciex Service Maintenance Agreement, Sciex cannot guarantee that the product is fitted with the most current mandatory engineering revisions or that you will receive the most current information bulletins concerning the product. If you purchased this product from a third party and would like further information concerning this topic, contact your Sciex representative.


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Chemical and Biological Safety

WARNING

Normal operation of the system can involve the use of solvents and reagents that are toxic, flammable, or biologically harmful.

• Observe all precautionary information printed on the original solution containers.

• Operate the system in the appropriate environment.

• Take all necessary precautions when using pathology or toxic materials to prevent the generation of aerosols.

• Observe all applicable precautionary procedures when using flammable solvents in or near the instrument.

• Wear appropriate laboratory attire, for example, safety glasses, gloves, lab coat and breathing apparatus, when working with hazardous materials.

• Dispose of all waste solutions in a proper manner.

Moving Parts or Sharp Objects

WARNING

Risk of personal injury. To avoid injury due to moving parts, observe the following:

• Never attempt to exchange labware, reagents, or tools while the instrument is operating.

• Never attempt to physically restrict any of the moving components of the instrument.

• Keep the instrument work area clear to prevent obstruction of the movement.

Moving parts are limited to the sample handling system. Plate movement is safety interlocked through the sample access cover. To avoid injury due to moving parts, observe the following:

• Keep loose clothing and hair away from the plate area.

• Never attempt to physically restrict movement of the plate assembly.


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Disposal of Devices Containing Mercury Components

This product contains a mercury-added part. Recycle or dispose of according to local, state, or federal laws. It is very important that you understand and comply with the safe and proper disposal of devices containing mercury components (switch, lamp, battery, relay, or electrode). The mercury component indicator label can vary depending on the type of device.

Electrical Safety

WARNING

A high voltage power supply is used with this instrument. Safety interlocks disable high-voltage output while the capillary access cover is open and remove the risk of shock while performing routine instrument functions. However, removal of any panel may expose an individual to the possibility of severe electrical shock and/or mechanical injury. For this reason, any service requiring removal of a panel or otherwise overriding or disabling safety interlocks must be done by Sciex personnel only.

To prevent electrically related injuries and property damage, properly inspect all electrical equipment prior to use and immediately report any electrical deficiencies. Contact a Sciex representative for any servicing of equipment requiring the removal of covers or panels.

To reduce risk of electrical shock, all devices employ a three-wire electrical cable and plug to connect the equipment to the electrical mains supply and protective earth.

• Make sure that the wall outlet receptacle is properly wired and connected to protective earth.

• Make sure there is clear access to the mains supply connector on the instrument. The connector must be accessible to disconnect the instrument from the electrical mains supply.

• Do not use a three-to-two wire plug adapter.

• Do not use a two-wire extension cord or a two-wire multiple-outlet power strip.

• Disconnect the electrical mains supply cord from the system before performing maintenance.

• Do not remove any panels; panels should be removed only by qualified service personnel.


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Laser Safety (for Optional Laser Device)

WARNING

The laser (optional) is designated as “Class 3B.” The “3B” classification means that “direct intrabeam viewing of this type of laser is always hazardous to personnel.”

The laser (optional) and several other integral components are housed in a sealed container that together comprise the laser assembly. The laser assembly has no user serviceable parts. Service of the laser assembly is restricted to certified Sciex Field Service Employees (FSEs).

During normal operation of the system, laser light is not accessible to the user. Therefore, the overall laser classification of the CE Instrument is “Class 1,” defined as “lasers which are safe under reasonably foreseeable conditions of operation.”

To prevent users from potentially harmful laser light, observe all safety warnings and NEVER REMOVE THE OUTER CASING OF THE LASER ASSEMBLY.

Safety Symbols and Labels

High Voltage Electric Shock Risk Symbol

This symbol indicates that there is high voltage and there is a risk of electric shock, and the operator should use care when accessing this area.

Attention Safety Symbol

This symbol calls attention to important information to read, or is accompanied by another symbol indicating a particular safety hazard. The information is located either on the label with the symbol or in the P/ACE MDQ plus system documentation.


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Sharp Object Label

This symbol indicates that there are sharp objects, and the operator should use care when accessing this area.

Laser Light (for Optional Laser Device)

This product may contain a laser-added part. A separate guide included with that part will indicate that potential hazard to your personal safety exists from a laser source. The laser indicator label can vary depending on the type of device.

Protective Earth or Ground Terminal

This symbol identifies the location of the protective earth or ground terminal on the equipment.

Fuse Warning Symbol

This symbol indicates that the instrument fuse may be replaced only with the specified type andrating.

Off Position of Convenience Switch

This symbol graphically represents the equipment convenience push-button switch when it is in the off position.


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On Position of Convenience Switch

This symbol graphically represents the equipment convenience push-button switch when it is in the on position.

For Research Use Only

This label indicates that the system is for research use only and should not be used in diagnostic procedures.

RoHS Notices

These labels and materials declaration table (the Table of Hazardous Substance’s Name and Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Products” requirements.

China RoHS Caution Label

This label indicates that the electronic information product contains certain toxic or hazardous substances. The center number is the Environmentally Friendly Use Period (EFUP) date, and indicates the number of calendar years the product can be in operation. Upon the expiration of the EFUP, the product must be immediately recycled. The circling arrows indicate the product is recyclable. The date code on the label or product indicates the date of manufacture.

China RoHS Environmental Label

This label indicates that the electronic information product does not contain any toxic or hazardous substances. The center “e” indicates the product is environmentally safe and does not have an Environmentally Friendly Use Period (EFUP) date. Therefore, it can safely be used


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indefinitely. The circling arrows indicate the product is recyclable. The date code on the label or product indicates the date of manufacture.

Other Instrument Labels

This section provides information for some labels and symbols appearing on the P/ACE MDQ plus instrument housing. These labels and symbols may be associated with user-serviceable procedures. Individual hazards associated with a specific procedure in this guide may use these labels and symbols, and are included in Warnings or Cautions within the procedures for that task.

Recycling Label (WEEE)

The symbol of a crossed-out wheeled bin on the product is required in accordance with the Waste Electrical and Electronic Equipment (WEEE) Directive of the European Union.

The presence of this marking on the product indicates:

• That the device was put on the European Market after August 13, 2005 and

• That the device is not to be disposed via the municipal waste collection system of any member state of the European Union.

For products under the requirement of WEEE directive, please contact your dealer or local Sciex office for the proper decontamination information and details on the take back program that facilitates proper collection, treatment, recovery, recycling, and safe disposal of this device.

CE Mark Label

The CE symbol is a conformity marking indicating that the P/ACE MDQ plus instrument meets the requirements of applicable European Conformity directives within the European Economic Area (EEA).


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RCM Label

The RCM symbol on the P/ACE MDQ plus instrument is an identification marking that indicates this instrument is compliant with the Australian Communications and Media Authority’s (ACMA) regulatory arrangements for telecommunications, radio, EMC and electromagnetic energy (EME) requirements. This symbol is required on this instrument for the Australian and New Zealand markets.

CSA Mark Label

The CSA symbol on the P/ACE MDQ plus instrument indicates that the instrument has been certified by a Nationally Recognized Testing Laboratory (NRTL) to applicable Laboratory Equipment Safety Standards for the United States and Canada.


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CHAPTER 2

Pre-Installation

About This Guide

This Site Planning Guide assists designated operators in identifying the items required for laboratory preparation so that the installation of the SCIEX P/ACE™ MDQ plus Capillary Electrophoresis System can be completed efficiently.

The supplies you must have on hand prior to the installation of the instrument are specified. Also provided is a list of accessories available.

For your convenience, a checklist is included to help you prepare for the installation.

Preparation Check List

The following is a summary of the items that must be addressed prior to installation. Mark each item as it is completed. When these prerequisites have been met, contact your local Sciex representative to schedule the installation.

• Appropriate table or bench (Recommended Bench Space on page 20)

• Suitable electrical mains supply (Table 4.1 on page 23)

• Suitable laboratory environment (Table 4.1 on page 23)

• Training prerequisites met (Training on page 27)

Technical Support

If you encounter a problem that is not discussed in this guide, and you need technical support, contact your Sciex representative using the information below.

NOTE When you call your local dealer or Sciex, be sure to have your registration material, instrument serial number, and software version number available.


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Pre-Installation Check List

The following is a list of requirements and items necessary for installation of the P/ACE MDQ plus system. Use this list to check off the requirements and items you have and other items as you receive them. To ensure a successful installation, please verify that you have these items prior to scheduling the installation with your local office.

P/ACE MDQ plus Instrument

• Electrical mains supply requirements

• Bench space

• Capillary Test Kit PN 33843 (must be ordered separately)

Computer

• Supplied by Sciex (or equivalent computer system)

• Windows 7

• P/ACE MDQ plus software with 32 Karat software

• Printer (Optional)

• GPIB communications interface (USB-GPIB cable)

Detectors

• UV/VIS detector

• PDA detector (if ordered)

• LIF detector (if ordered)

Table 2.1 Technical Support Information

Instrument Serial Number:

P/ACE MDQ plus Software Version:

Firmware Version:

Dealer Name:

Dealer Phone Number:

Mail AB Sciex500 Old Connecticut PathFramingham, MA 01701 USA

Web Address: www.sciex.com


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Options

• Filters (spare for each filter)

• Lasers (if ordered)

• External detector adapter (if ordered)


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CHAPTER 3

Site Requirements

Physical Layout Requirements

The SCIEX P/ACE™ MDQ plus system consists of the P/ACE MDQ plus instrument, a computer, 22 inch monitor, keyboard, and mouse. Instrument dimensions are shown in Figure 3.1

Figure 3.1 P/ACE MDQ plus Instrument Dimensions


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Recommended Bench Space

In addition to space for the instrument itself, additional space for the computer and printer (if any) is required.

Laboratory Layout

Figure 3.2 illustrates the recommended system setup.

Figure 3.2 Typical P/ACE MDQ plus System Setup

Ventilation Requirements

Adequate space must be provided around instrument for ventilation and access to the communications connector. Allow least 4 inches clearance on all sides.

Access to Mains Supply Connector

Make sure there is clear access to the mains supply connector on the instrument. The connector must be accessible to disconnect the instrument from the electrical mains supply.

Table 3.1 Minimum Bench Footprint

Length 1.8 m (6 feet)

Width 91.4 cm (3 feet)

Height Allowance 1.07 m (3 1/2 feet)

Supportable Weight 227 kg (500 lbs)


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Other Environmental Requirements

Refer to Table 4.1 P/ACE MDQ plus Instrument Specifications on page 23 for humidity, altitude, temperature requirements.

Electrical Requirements

The P/ACE MDQ plus system is shipped with a mains supply cord suitable for North America (nominal 120 VAC, 60 Hz) installations. A mains supply cord suitable for non-North America installations will be provided by Sciex. The instrument requires one grounded electrical mains supply outlet.

The electrical mains supply to the laboratory should connect directly from an electrical line transformer at a supply known to be clear of erratic power loads, spikes, and electromagnetic interference. Electrical supplies reserved for the instrument must have adequate reserve capacity. Normal loading should not exceed 50% of nominal capacity to allow for start-up loads and the addition of new instruments.

A total of three grounded electrical mains supply outlets are required for the computer, the monitor and the P/ACE MDQ plus system. Any additional accessories, such as a printer, will require additional electrical mains supply outlets.


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CHAPTER 4

Specifications

Use this information to prepare the environment where the system is to be located.

P/ACE MDQ™ plus Instrument Specifications

Table 4.1 P/ACE MDQ plus Instrument Specifications

Specification Type Description

Weight 85.3 kg (188 lbs)

Firmware Version 10.2 or higher

Electrical Mains Supply Requirements Supply voltage must not exceed 10% of nominal

P/ACE MDQ plus instrument 100 VAC to 240 VAC, 8.0 A, 50/60 Hz; auto-sensing voltage power supply

PC (typical) 6.0 A, 50/60 Hz

Monitor (22 inch) 1.5 A, 50/60 Hz

Fuses T8.0 A 250 V 5 mm x 20 mm glass cartridge (2 ea.)

Maximum Heat Dissipation (to room) 400 W (1365 BTU/hour)

Ambient Operating Temperature Range

Operational: 5°C to 40°C (41°F to 104°F)Recommended for performance: 15°C to 30°C (59°F to 86°F)

Relative Humidity Range <80% (non-condensing) up to 30°C (59°F), decreasing linearly to 50% at 40°C (104°F)

Altitude Restrictions Up to 2000 m (6562 ft)

Installation Category Category II

Pollution Degree 2

Sound Pressure Level Maximum: 75 dBMaximum at 1 meter: 66 dB


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Sample Temperature Control

Capillary Temperature Control

Pressure and Vacuum System

Table 4.2 Sample Temperature Control Specifications


Temperature Range From 20°C below ambient to 60°C (140°F)Minimum setting: 4°C (39°F)

Temperature Stability ±1°C at 25°C ±3°C at 4°C and 60°C

Temperature Accuracy ±2°C within ±15°C of ambient temperature±3°C outside of ±15°C of ambient temperature

Response Time Temperatures between ambient and 20°C below ambient: 120 minutes Temperatures between ambient and 60°C (140°F): 120 minutes

Table 4.3 Capillary Temperature Control Specifications


Temperature Range From 10°C belowambient to 60°C (140°F)Minimum setting: 15°C (59°F)

Temperature Stability ±1°C at 25°C

Temperature Accuracy ±1°C within ±5°C of ambient temperature±2°C outside of ±5°C of ambient temperature

Response Time Temperatures between ambient and 10°C below ambient: 45 minutes Temperatures between ambient and 60°C (140°F): 45 minutes

Table 4.4 Pressure and Vacuum System Specifications


Pressure Range Injection: 0.1 psig to 25 psig (pressure) or 0.1 psig to 5.0 psig (vacuum)Rinse: 0.1 psig to 100.0 psig (pressure) or 0.1 psig to 5.0 psig (vacuum)

Pressure Accuracy ±0.3 psi at 25 psi±1.0 psi at 100 psi

Pressure Direction Applied at inlet or outlet for all pressure functions, rinses and injections. User-selectable from software.


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Computer Requirements

IMPORTANT Users must have Administrator or Power user permission on the local workstation.

IMPORTANT Install 32 Karat software in the C:\32Karat folder.

Detector Specifications

UV Detector

Table 4.5 Computer Specifications

Item Description

CPU Lenovo PC (validated on M82 model)

Memory 4 GB

Hard Drive 320 GB or larger

CD/DVD RW Required

USB Ports 2 open ports (one for license key and one for GPIB)

Monitor Size: 22 inch Resolution: 1680 x 1050Color: True Color

Keyboard and Mouse Microsoft compatible

Interface GPIB Data Communications Interface USB cable (National Instruments)

Operating System Microsoft Windows 7 (32-bit) with Service Pack 1

Software P/ACE MDQ plus software version 10.2 (includes 32 Karat software)

Table 4.6 UV Detector Specifications


Filter Selection 200 nm, 214 nm, 230 nm, and 254 nm (standard) with three open positions for additional filters.

Filter Dimensions Diameter: 0.5 inch (1.27 cm)Thickness: 0.20 inch (5 mm)

Filter Bandwidth 10 nm nominal

UV Source 30 W pre-aligned deuterium lamp

UV Source Lifetime 1000 hours


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Photo Diode Array (PDA) Detector (Optional)

Laser Induced Fluorescence (LIF) Detector (Optional)

Table 4.7 PDA Detector Specifications


UV Source 30 W pre-aligned deuterium lamp

UV Source Lifetime 1000 hours

Detector Wavelength Range 190 nm to 600 nm

Detector 256 element diode array

Detector Wavelength Accuracy 2 nm

Bandwidth 6 nm minimum (absorbance averaging)

Scan Collection Frequency 0.5 Hz to 32 Hz

Table 4.8 LIF Detector Specifications


Relative Fluorescence Units (RFU) Range

0 RFU to 1000 RFU

Dynamic Range (at a dynamic range setting of 1000)

>104

Sensitivity 1 x 10-11 1 M sodium fluorescein with a signal-to noise ratio >2

Baseline Noise <0.005 RFU peak to peak (Specifications are for an OPCAL with probe guide/lens.)

Baseline Drift <0.2 RFU per hour

Wavelength Ranges (for optics) Excitation: 300 nm to 700 nmEmission: 350 nm to 750 nm

Filters (optional) For 488 nm laser: 488 nm notch filter and 520 nm band-pass filter

For user-supplied lasers: Two filters are required: a laser filter to block stray laser light and an emission filter to select the wavelength of the emitted light. Both filters must have an outside diameter of 0.500 inch (+0.000 inch, -0.010 inch) and a thickness 0.350 inches. If multiple filters are used in a single channel, the total thickness cannot exceed 0.350 inches.

Laser 3 mW, 488 nm solid state laser


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CHAPTER 5

Training

Pre-Training Requirements

After the system has been installed, a Sciex Field Service Employee will train up to two operators in basic instrument operation.

Successful implementation of the installation is dependent upon the training environment. To ensure a focused and optimized training session, follow these guidelines:

1. It is important that the operators are available and uninterrupted throughout the initial training period.

2. No more than two operators can be trained on the instrument at any given time.

System operation requires a basic knowledge of Windows 7. The operator must have the following skills:

• Use Add/Remove programs

• Create, open, save, edit, move, and copy files

• Perform disk management operations

Additional Training

Our training program has been designed in phases to allow operators to focus on and master basic operational elements. The first phase involves the use of the P/ACE MDQ plus system electronic guides.


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Revision History

Initial Issue, B54950AA, February 2015P/ACE MDQ plus Software version 10.2.x (includes 32 Karat software)P/ACE MDQ plus Firmware version 10.2.x

This guide applies to the latest software and firmware listed above, and any higher subsequent versions. When a subsequent software or firmware version affects the information in this guide, a new issue will be released to the Sciex web site. For updates, go to www.sciex.com and download the latest version of the guide.

http://www.sciex.com


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Documents

P/ACE MDQ plus Capillary Electrophoresis System Site Planning