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P15611 Digital Microfluidics Packaging 1 Operations Manual
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Table of Contents:
Getting Started:..............................................................................................pg 3 Fluid Delivery:................................................................................................pg 4 Thermoelectric Plate Setup:..........................................................................pg 5 Enclosure Sensor Outputs:.............................................................................pg 6 Running the Controller:...................................................................................pg 7
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Getting Started:
1. Set up the DMF chip on the chip stage using the copper shim for separation between the substrates.
2. Set the chip stage inside of the enclosure on the designated recessed cutout.
3. Place the lug of the ground wire over the hole on the copper shim and stage. And fasten using (1) M3 x 4mm Lg flat head cap screw.
4. Ensure no wires will impede the device from closing, and close the device. Make sure you hear a “clicking” sound from the latch making contact with the metal plate.
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Fluid Delivery:
1. Remove the plug from the desired delivery port (Use a flat object like a flat head screwdriver to make this easier), and replace with a tip leader attachment.
2. Select the appropriate pipette depending on the desired amount of fluid to be delivered.
3. Attach an Eppendorf microloader pipette tip to the pipette and draw the fluid to be delivered.
4. Slowly insert the pipette tip into the enclosure through the tip leader and guide to the location on the chip.
5. Deliver the droplet and slowly pull the pipette tip out of the enclosure.
Click on the following links successively to view the steps for fluid delivery: https://edge.rit.edu/edge/P15611/public/Photo%20Gallery/mloader.gif https://edge.rit.edu/edge/P15611/public/Photo%20Gallery/drawfluid.gif https://edge.rit.edu/edge/P15611/public/Photo%20Gallery/deliverdrop.gif
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Thermoelectric Plate Set-up: See page 37 in tc4820_manual.pdf for controller/peltier plate hookup diagram for power. Reference TE1271_42_5.pdf for peltier plate cold side vs hot side hookup. See page 43 in the tc4820_manual.pdf for computer RS232 communications hookup. Using the controller with computer RS232 connection: See pages 26 to 35, tc4820_manual.pdf Fan Hookup Diagram:
tc4820_manual.pdf http://edge.rit.edu/edge/P15611/public/Detailed%20Design%20Documents/Environmental%20Controls/tc4820_manual.pdf TE1271_42_5.pdf http://edge.rit.edu/edge/P15611/public/Detailed%20Design%20Documents/Environmental%20Controls/TE1271_42_5.pdf?rev=0
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Enclosure Sensor Outputs: 1. Connect the db9 cable from the Microfluidics Enclosure to the Electronics Housing.
2. Connect the USB to first port from the left on the enclosure to the USB terminal on the computer.
Load “Continuous Serial Write and Read.vi” and select COM 3 as the port to read and write from. Run the VI and select the Read Button on the VI to start reading from the sensor. If dialogue if blank or HTU21D Sensor not found, indicates that arduino board should be reset or sensor not working properly.
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Running the Controller:
1. If not already done, install the controller software using the supplied CD. Note: LabVIEW software will be required since the TC-48-20.exe is actually an executable version of a LabVIEW .vi file.
2. Navigate to the default location of the file and double click the .exe file (default location is C:\Program Files\TE Technology\TC-48-20\TC-48-20.exe). You will see the following screen:
3. Click on the “COMMUNICATIONS PORT” and select the port to which the controller is connected then click “CONNECT”.
4. Click on the “CONTROL TEMPERATURE” box to set the desired temperature. Note: The “CONTROL MODE” box allows you to select either cooling or heating which coincides with how the plate was set up.
5. Run the VI.
For a more detailed description of the controller operation, please refer to the tc4820_manual.pdf
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