0

20

40

60

0 2 4 6 8

02468

1012

0 2 4 6 8

Oxa

late

(µ

mo

l/gF

W)

Itaconate (µmol/gFW)

r=-0.702**

Citr

ate

(µ

mo

l/gF

W)

r=0.389

0.000.020.040.060.080.100.12

0 2 4 6 8Is

oci

tra

te (

µm

ol/g

FW

)

0.0

0.5

1.0

1.5

2.0

0 2 4 6 8

Asc

orb

ate

(µ

mo

l/gF

W)

r=0.052

r=0.195

New Leaves

10 mM Itaconate 1 weeks 10 mM Itaconate 2 weeks 10 mM Itaconate 3 weeks

Itaconate (µmol/gFW)

Supplementary Data 1 Miyagi et al.

-2

-1

0

1

2

3

-2 -1 0 1 2 3

PC 1 (35.1 %)

PC 2 (18.1 %)

-0.06

-0.04

-0.02

0.00

0.02

0.04

0.06

-0.08-0.06-0.04-0.020.000.020.040.060.080.10

Stems

-2

-1

0

1

2

3

-3 -2 -1 0 1 2 3

-2

-1

0

1

2

3

-2 -1 0 1 2 3-0.15

-0.10

-0.05

0.00

0.05

0.10

0.15

0.20

: Organic acid: Amino acid: Phosphorylated

PC 2 (18.1 %)

PC 1 (35.1 %)

Iso

citr

ate

Orn

ithin

eA

sco

rba

teR

uBP

Cou

mar

ate

6P

GA

con

itate

Gly

FB

PA

spS

hiki

ma

teP

yru

vate

Suc

cin

ate

Citr

ate

Cys

Trp

Ser

Ala

DH

AP

G6P

Ru5

PR

5PG

luC

inn

am

ate

Citr

ulli

ne

GA

BA

Tyr

Phe

Le

uL

ysV

alIleA

snA

rgT

hr

Me

tP

roH

is2

OG

PE

P3

PG

AO

xala

teG

1PG

lnM

ala

teF

um

ara

te

Iso

citr

ate

Orn

ithin

eA

sco

rba

teR

uBP

Cou

mar

ate

6P

GA

con

itate

Gly

FB

PA

spS

hiki

ma

teP

yru

vate

Suc

cin

ate

Citr

ate

Cys

Trp

Ser

Ala

DH

AP

G6P

Ru5

PR

5PG

luC

inn

am

ate

Citr

ulli

ne

GA

BA

Tyr

Phe

Le

uL

ysV

alIleA

snA

rgT

hr

Me

tP

roH

is2

OG

PE

P3

PG

AO

xala

teG

1PG

lnM

ala

teF

um

ara

te

Iso

citr

ate

Orn

ithin

eA

sco

rba

teR

uBP

Cou

mar

ate

6P

GA

con

itate

Gly

FB

PA

spS

hiki

ma

teP

yru

vate

Suc

cin

ate

Citr

ate

Cys

Trp

Ser

Ala

DH

AP

G6P

Ru5

PR

5PG

luC

inn

am

ate

Citr

ulli

ne

GA

BA

Tyr

Phe

Le

uL

ysV

alIleA

snA

rgT

hr

Me

tP

roH

is2

OG

PE

P3

PG

AO

xala

teG

1PG

lnM

ala

teF

um

ara

te

PC 1 loadings

PC 2 loadings

PC 3 loadings

PC 3 (10.4%)

PC 3 (10.4%)

Control 0 week Control 1 week Control 2 weeks Control 3 weeks

10 mM Itaconate 0 week 10 mM Itaconate 1 weeks 10 mM Itaconate 2 weeks 10 mM Itaconate 3 weeks

a

b

c

d

e

f

Supplementary Data 2 Miyagi et al.

compound

0

1

2

3

0 1 2 3 4

0

1

2

3

4

0 1 2 3 4

0

20

40

60

0 1 2 3 4

0.00

0.01

0.02

0.03

0.04

0 1 2 3 4

Oxa

late

(µ

mo

l/gF

W)

Itaconate (µmol/gFW)

r=-0.113

Citr

ate

(µ

mo

l/gF

W)

r=-0.058

Iso

citr

ate

(µ

mo

l/gF

W)

Asc

orb

ate

(µ

mo

l/gF

W)

r=-0.488*

r=-0.396

Stems

10 mM Itaconate 0 week 10 mM Itaconate 1 weeks 10 mM Itaconate 2 weeks 10 mM Itaconate 3 weeks

Itaconate (µmol/gFW)

Supplementary Data 3 Miyagi et al.