OS 213LAB 03: PARAGONIMUS WESTERMANI

OS 210LEC 0X: TITLE

OS 213: Human Disease and Treatment 3(Circulation and Respiration)LAB 03: PARAGONIMUS WESTERMANIExam 2 | Parasitology Faculty | August 17, 2012

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\\JM, Josh, Jer, ShamUPCM 2016 B: XVI, Walang Kapantay!1of 3

OUTLINEI. OvumII. VectorsA. Primary: AntemelaniaB. Secondary: SundathelpusaIII. MetacercariaIV. AdultV. Adult Reproductive OrgansA. Female Reproductive OrganB. Male Reproductive OrganVI. Other StructuresVII. Life CycleVIII. Actual Lab Specimen

OVUM Oval, yellowish-brown, thick-shelled 80-118 micrometers by 48-60 micrometers Flat but prominent operculum (larger end) Abopercular portion is thickened (smaller end) Unembryonated at oviposition Cystein proteinase of the cathepsin family in immature eggs Immature egg embryonates in water, moist soil or feces Miracidium develops within 2-7 weeks

Figure 1.Ovum

VECTORS

Primary: Antemelania First intermediate host: the snails Antemelania asperata; Antemelania dactylus Miracidium enters snail one sporocyst stage 2 redial stage cercaria emerges exit snail to infect 20 host Cercaria Covered with spines and has an ellipsoidal body with small tail Stylet present at dorsal side of the oral sucker

Figure 2. Antemelania

Secondary: Sundathelphusa Second intermediate host: mountain crab Sundathelpusa philippina Cercaria penetrates soft parts Encysts metacercaria in the gills, body muscles, viscera, legs Crab may be infected by eating infected snails

Figure 3. Sundathelphusa

METACERCARIA Definitive host (man) acquires infection by eating raw or insufficiently cooked crabs harboring metacercaria Metacercaria is round and measures from 381-457 micrometers After ingestion, metacercaria excysts in duodenum of host Cysteine proteinases secreted by metacercarias excretory bladder help in excystment and host immune modulation Adolescent worm then traverses through the intestinal wall, into the peritoneal cavity, where it wanders about and embeds itself in the abdominal wall for several days Afterwards, returns to coelom, migrates through diaphragm to pleural cavity where it develops into adulthood Completion of development in the definitive host: 65-90 days Can persists in man up to 20 years or more Can also infect dogs, cats, rats

Figure 4. Metacecaria

Figure 5. Actual Metacercaria Specimen

ADULT An adult may be as large as a coffee bean, hence it may be appreciated grossly Adults usually measure 7-12mm long, 4-6mm wide, and are 3.5-5mm thick Rounded anteriorly, slightly tapered posteriorly Tegument covered with single-spaced spines Two testes are lobed, midway between ventral sucker Ovary located anterior to testes but posterior to ventral sucker Vitellaria are branched extensively Found in pairs, threes in fibrotic capsules or cysts in the lungs of hosts Capsules have openings that allows eggs to escape; transported by ciliary epithelium along with lung exudates In the pharynx, the eggs are either swallowed or coughed out They are hermaphroditic

Figure 6.Adult

Figure 7. Paragonimus sp. Adult Lung Fluke.(Key: AC=acetabulum (ventral sucker); OV=ovary; CE=cecum; TE=testes; OS=oral sucker; UT=uterus; EB=excretory bladder)

ADULT REPRODUCTIVE ORGANS

Female Genitalia

Figure 8. Female Genitalia(Key: red/left arrow= Uterus distended with eggs; black circle=ventral sucker which is faintly stained; blue/right arrow=ovary)

Figure 9.Uterus in focus

Male Genitalia

Figure 10.Testes(Key: Paired lobulated pink structures)

OTHER STRUCTURES

Figure 11. Other Paragonimus Structures(Key: orange/upper left horizontal arrow=cecum; blue/upper left vertical arrow=uterus; violet/upper right arrow=ovary; yellow/lower right arrow=testes; surrounding green=vitellaria)

LIFE CYCLEFigure 12. Paragonimus Life Cycle

ACTUAL LAB SPECIMEN OF ADULT P. WESTERMANI

Figure 13. Head Part with oral sucker

Figure 14. Uterus

Figure 15. Paired Testis

Figure 16. Caudal part with caecum

END OF TRANSCRIPTIONGreetings:

JM: If you can dream it, you can do it! Walt Disney.Santolians, we can do this! Hi to Gerdys, Mentoring Grp, Sausage Fest, Blocks A and B. God bless us all!

Josh:

Sham:

Jer: Hi Shammmmmm!!! XDTransersUPCM 2016: XVI, Walang Kapantay!3of 3

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