A cost-efficient micro fluidic test carrier and easy to store. This requires a sable). Counteracting centrifugal and

enables a dependable quantification of miniaturization of the optic unit and capillary forces are used to transport

different parameter concentrations by therefore, a folding of the light ray path them to different reservoirs without the

analyzing simultaneously optical into the plane of the disc (in-plane- help of pumps and valves and hold them

measurements of dry chemical tests e.g. detection). Optical features such as

in medical practices and intensive care mirrors and shutters are integrated at the

units. For the development of the optical same time as the micro fluidic ones by

measuring and analysis unit different using injection molding.

constraints have to be considered. The

test chemistry is selected in a way that it For the spectroscopic analysis of a para-

reacts optically to the concentration of the meter, two different wavelengths are

parameters measured. Main parameters necessary. In addition to the measuring

wavelength a reference there for defined reaction times. Each

wavelength is used to trial requires only a 10 min measurement

correct the influence of time. This processing includes the

scattering from the test application of the blood sample, the

chemistry on absorption centrifugation of the erythrocytes, and

tests. LEDs are used as a the dosing and aqueous dilution of the

cost-efficient and fast remaining plasma and the dissolution of

modulated illumination the freeze-dried reagents with the

unit. Photodiodes are homogenized sample.

used for the detection.

T h e e n t i r e o p t i c a l

module is thereby loca-

ted on the front part of

the disc. The back part is

left free for the rotary

drive, a carrier for the

d i s p o s a b l e a n d a

temperature control if

needed. The current

design as well includes

an amplifying electronic

unit directly located at

the photodiode to reme-such as cholesterol and hsCRP for risk dy deviations and parasitic errors.assessments of heart attacks can be

determined that way. In order to comply Blood and diluents (buffer) are dosed and with the requirements of an application applied separately to the center of an in the PoC range, both measuring injection molded plastic disc (dispo-instrument and disposable must be small

Optical blood analysesfor cardiac risk diagnostic at the Point-of-CareAuthors:Daniel Rohleder, Dioptic GmbH, Weinheim (Germany)Valérie Winckler-Desprez, Roche Diagnostics GmbH, Mannheim (Germany)Christoph Klaunick, thinXXS Microtechnology AG, Zweibrücken (Germany)

Patient-side Point-of-Care blood analyzing provides lifesaving information in emergency situations. PoC-Tests help to control the progress of major diseases, save time and reduce the effort of the medical staff. The significance of the diagnostic conclusion is increased by analyzing specific combinations of biomarkers at the same time. Therefore, in the German BMBF-funded collaborative project 'µ-Kardio', a fast optical multi-parameters test is developed.

Notice: This is a summary of an article from „BioPhotonik“, special edition 1/2011, April 2011. Approved by the main author, Dr. Rohleder. You will find the complete article at www.bio-photonik.de (webcode B1001)

Detection

Mixing

Measurecircuit withmore than2000 measuringpositions

Microfluidic testting cartridge of thinXXS with dosing in the inner ring, followed by concentric mixing and reaction chambers as well as cuvettes lying outside. The in-plane detection takes place on the yellow marked circle.

Principle of the In-Plane-Detection

Pipette port

Measuringcuvette

vent

reflectingsurfaces

referencecuvette

Layout of a first disposable version for testing the optical functionality in rotation with reflecting surfaces as well as measuring and reference cuvettes.