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Prokaryotic control of genome expression 2 levels of control
Change in # of enzyme molecules produced Change in activity of enzymes present
Ex: feedback inhibition Genes turned on/off based on metabolic needs:
operons Operator + promoter + group of genes Often include several enzymes w/ in a pathway
* Regulator gene - a gene that codes for a repressor protein molecule. The repressor molecule binds to the operator and prevents RNA polymerase from binding to the promoter.
* Promoter - a short sequence of DNA where RNA polymerase first attaches when a gene is to be transcribed.
* Operator - a short sequence of DNA where the repressor binds, preventing RNA polymerase from attaching to the promoter.
* In addition, the part of chromosome (in eukaryotic cells) containing the gene must first decompact to form a "chromosome puffs" before the beginning of transcription.
2 Types of operons
Inducible: Default = off Inducer turns transciption on ex: an enzymatic pathway that allows the cell
to break down lactose Repressible:
Default = on Corepressor turns transcription off Ex: an enzymatic pathway that allows the cell
to synthesize tryptophan
The Lac Operon
Bacteria can use lactose if lactose is present and if there’s not enough glucose
B-galactosidase breaks down lactose Lactose present = transcription is on
Allolactose (inducer) inactivates the repressor Inactive repressor falls off of the operatorRNA polymerase binds
http://www.csam.montclair.edu/%7Esmalley/LacOperon.mov
The trp operon
Cell can get tryptophan from environment or by synthesizing it Will only synthesize tryptophan if there isn’t
any in the environment Tryptohan in the environment = no transcription
Tryptophan activates repressorTryptophan is a corepressorRepressor is from regulatory gene (trpR)
Repressor binds to operatorBlocks RNA polymerase
Positive gene regulation
Ex: lac operon transcription also depends on [glucose]
CRP: activates transcription (positive regulation) glucose cAMP cAMP activates CRP
Explain the events that would occur if. . . A cell was in an environment with plenty of
glucose but no lactose, and lactose was added
A cell was in an environment with tryptophan, then the tryptophan disappeared
A cell was in an environment with lactose and plenty of glucose, then the glucose disappeared