Operation Manual - GYROZEN · 2019. 7. 5. · 6.1 Inspection - Fixed Angle Rotor - 80 6.2 Inspection - Swing-out Rotor - 81 6.3 Inspection - Bucket - 81 6.4 Inspection - Adapter -

Operation Manual

ROTORS, ACCESSORIES for

GYROZEN Centrifuges

Date of Purchase

Where Purchased

0.Safety ----------------------------------------------------------- 4

1. Rotors ---------------------------------------------------------- 51.1 Fixed Angle Rotor - 51.2 Swing-out Rotor - 51.3 Fixed Angle Rotor Structure - 61.4 Swing-out Rotor Structure - 81.5 Fixed Angle Rotor Chart - 101.6 Fixed Angle Rotor Specifications - 111.7 Adapters for Fixed Angle Rotors - 141.8 Tube Recommendations for Fixed Angle Rotor - 261.9 Swing-out Rotor Chart - 301.10 Swing-out Rotor Specifications - 311.11 Buckets for Swing-out Rotors - 311.12 Tube Recommendations for Swing-out Rotors - 52

2. Preparing and Using Angle Rotors -------------------- 682.1 Preparing the Rotor - 682.2 Using Tubes - 682.3 Installing the Rotor - 692.4 Removing the Rotor/Sample - 69

3. Preparing and Using Swing-out Rotors -------------- 703.1 Preparing the Rotor - 703.2 Installing the Rotor - 703.3 Loading the Bucket/Adapter/Sample - 713.4 Removing the Rotor/Tubes - 71

4. Bucket and Adapter --------------------------------------- 724.1 Adapter for Fixed Angle Rotor - 724.2 Using Adapter for Fixed Angle Rotor - 734.3 Bucket - 744.4 Using Bucket - 754.5 Adapter for Swing-out Rotor - 764.6 Using Adapters for Swing-out Rotor - 77

5. Tubes, Bottles ------------------------------------------ 795.1 Using Tubes, Bottle - 795.2 Filling Volume - 795.4 Tube/Bottle Sterilization - 79

6. Maintenance ------------------------------------------- 806.1 Inspection - Fixed Angle Rotor - 806.2 Inspection - Swing-out Rotor - 816.3 Inspection - Bucket - 816.4 Inspection - Adapter - 826.5 Storage - Fixed Angle Rotor - 836.6 Storage - Swing-out Rotor - 836.7 Cleaning - Rotor - 846.8 Sterilizing Rotor - 856.9 Cleaning - Bucket - 866.10 Sterilizing - Bucket - 866.11 Cleaning - Adapter - 876.12 Sterilizing Adapter - 87

7. Troubleshooting -------------------------------------- 887.1 Rotor - 887.2 Tube - 89

Appendix 1Centrifugation Separations - 90

Appendix 2RCF calculation - 94Separation time - 94

Appendix 3chemical resistance of Plastics - 95

Appendix 4Chemical resistance of Aluminum - 96

www.GYROZEN.com4 / 96

0. Safety

• Check the rotor, bucket, and tube are free of scratch and crack before operation.• Check the chemical resistance chart(Refer to Appendix3&4) and do not use any samples inapplicable to the tubes and accessories.• Do not use any samples that may generate volatile or explosive vapor.• Always use the same type of tubes. (weight, material,density and volume.)• Do not exceed the maximum rated speed of the rotors, buckets and tubes in use.• The maximum speed of the rotor may vary depending on the device or tube or Adapter.• Make sure that there is no foreign material in the chamber.• Be careful not to load the rotor with too much force.• Firmly tighten the rotor onto the motorshaft.• Remove the rotor from the chamber after centrifugation.• After using the rotor, remove the lid from the rotor body. Keep the rotor upside down.• Do not put the rotor in the boiling water for sterilize. • There is a rotor ID. magnetic in the rotor drive hole which is located on the bottom of the rotor. Make sure not to damage the rotor ID. magnetic when handling the rotor. • Only use rotors, parts, and accessories provided by GYROZEN. GYROZEN. is not responsible for damages of the device and accidents caused by using parts and accessories not recommended.• Inspect the rotor and accessories each time after using them. If you find any abnormality, stop using them and contact GYROZEN.• It is your responsibility to sterilize and/or decontaminate the centrifuge and accessories before requesting service by GYROZEN.• Maximum speeds are based on a solution density of 1.2 g/mL in all rotors

Rotors and accessories are may damaged in repeated use or careless handling. And damage may be worsened under centrifugal forces. Therefore even a small defect on the rotor or accessories may cause personal injury and instrument damage. Carefully read and understand this manual.

Technical Support : +82-2-3452-8966 / [email protected] 5 / 96

1. Rotors

Fixed angle rotor holds tubes at an angle to the axis of rotation. It is designed of the aluminum material, so fixed-angle rotor can withstand much higher gravitational forces than swing-out rotor. It is useful for pelleting subcellular particles and remove particles from a suspension and discard the debris, and isopycnic banding of DNA.

Swing-out holds tubes in the buckets(or Adapters) which swing horizontally.It is useful when samples are to be resolved in density gradients. The longer path length permits better separation of individual particle types from a mixture.

1.1 Fixed Angle Rotor

1.2 Swing-out Rotor

Supernatant

Pellet

Pellet Centrifugal Field

Fixed Angle Rotor

Swing-out Rotor

Differential Centrifugation Density Gradient Centrifugation


① Rotor Hole: Tube is inserted into the rotor hole.② Lid: Lid prevents tubes or parts from being bounced during centrifugation.③ Lid Handle: After loading the rotor, turn the rotor handle clockwise to tighten the rotor.④ Rotor Body: Body protects tubes and samples.⑤ Drive hole: Drive hole is where the motor shaft is connected.

②

③

④

①

⑤

1. Rotors

1.3 Fixed-angle Rotor Structure


③

1. Rotors

1.3 Fixed-angle Rotor Structure

① Rotor bolt: After loading the rotor turn the rotor bolt clockwise to tighten the rotor.② Sleeve: Tube is inserted into the sleeve③ Drive hole: Drive hole is where the motor shaft is connected.

①

②


① Pivot: Pivot is a hook that can hang bucket.② Rotor bolt: After loading the rotor turn the rotor bolt clockwise to tighten the rotor.③ Drive hole: Drive hole is where the motor shaft is connected.

① Plate bucket: Plate bucket holds microplate.② Rotor bolt: After loading the rotor, turn the rotor bolt clockwise to tighten the rotor.③ Drive hole: Drive hole is where the motor shaft is connected.

[4-place Swing-out rotor][6-place Swing-out rotor]

[2-place Swing-out rotor : For Microplates]

1. Rotors

1.4 Swing-out Rotors Structure

③

①

①

②

②

③


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www.GYROZEN.com10/96

1. RotorsRo

tor t

otal

capa

city

Speed/Max.RPM

1.5 Fixed-angle Rotor Chart (RPM and Capacity)

5K 10K 15K 20K 22K

GRF-L-1000-6

GRF-L-1000-4

GRF-L-500-6

GRF-L-250-6GRF-G-250-6GRF-B-250-6

GRF-L-85-6GRF-G-85-6GRF-B-85-6


GRF-L-c50-6

GRF-L-15c-12GRF-L-15-12GRF-G-c15-12

GRF-B-c15-12

GRF-B-15-12

GRF-G(B)-c50-6 GRF-L-50-6GRF-SR-50-6GRF-SR-c50-6

GRF-B-15-24GRF-G-15-24

GRA-SR-15-24AGRA-G-15-24

GRA-S-15-16A

GRA-15/10-12

GRA-15-6AGRF-B-m5.0-20

100

200

300

400

500

1,500

3,000

4,000

6,000

Technical Support : +82-2-3452-8966 / [email protected] 11/96

1. Rotors

1.6 Fixed-angle Specifications

-For Low Speed, Clinical-

Rotor Shape

Cat. No. GRA-15-6A GRA-15/10-12 GRA-S-15-16A GRA-SR-15-24 GRF-SR-50-6 GRF-SR-c50-6

Max. Capacity 6 x 15 mL 6 x 15 mL12 x 10 mL 16 x 15 mL 24 x 15 mL 6 x 50 mL 6 x 50 mL conical

Hole angle ∠45° ∠45° ∠45° ∠45° ∠25° ∠25°

Rotor hole dimension (mm)

18 x 90 (Sleeve)

18 x 90 (Sleeve)

18 x 90 (Sleeve)

18 x 90 (Sleeve) 29.5 x 95.4 30 x 108.6

Rotor hole bottom type

Flat with rubber pad

(Sleeve)


(Sleeve)


(Sleeve)


(Sleeve)Round Conical

Max. height for tube fit (mm)

125 (120 for conical/

widercap)

125(120 for conical/

widercap)


widercap)


widercap)120 125

Max. Radius* (mm) 116 116 151.5 138(Inner)155(Outer) 95 98.4

406

Max. RPM(rpm)Max. RCF(xg)

4,0002,075

4,0002,075

416 4,0002,700

624R 4,0002,469(I)/2,773(O)

6,0003,824

6,0003,960


- For Large Capacity/High Speed Centrifuge -

- For Multi prupose, High Speed Centrifuge -

1. Rotors


-Supra/Combi Series 로터-

Rotor Shape

Cat. No. GRF-L-15-12 GRF-L-c15-12 GRF-L-50-6 GRF-L-c50-6 GRF-L-50-8 GRF-L-85-6 GRF-L-250-6

Max. Capacity 12 x 15 mL 12 x 15 mL conical 6 x 50 mL 6 x 50 mL

conical 8 x 50 mL 6 x 85 mL 6 x 250 mL6 x 15 mL

Hole angle ∠30° ∠25° ∠30° ∠25° ∠30° ∠25° ∠25°Rotor hole dimension (mm) 17 x 93.2 17 x 107.2 29.5 x 92.2 30 x 108.6 29.5 x 93.2 38.5 x 100 62x100/17x94

(250/15)Rotor Hole Bottom type Round Conical Round Conical Round Round Flat/Round

Max. height for tube fit (mm) 125 125 120 125 120 125 130/120

(250/15)

Max.Radius (mm) 100 103 100 98.4 103 99.5 134

1736RMax. RPM(rpm)Max. RCF(xg)

17,00032,310

15,00025,910

17,00032,310

15,00024,753

15,00025,910

15,00025,029

10,00014,981

2236R 20,00044,720

20,00046,062

22,00054,111

17,00031,793

20,00046,062

20,00044,496

13,00025,318

Rotor Shape

Cat. No. GRF-G-c15-12Not for 1248 GRF-B-c15-12 GRF-B-15-12 GRF-G-15-24A GRF-B-15-24 GRF-G-15-24

Not for 1248GRF-G-c50-6Not for 1248

Max. Capacity 12 x 15 mL 12 x 15 mL conical 12 x 15 mL 24 x 15 mL 24 x 15 mL 24 x 15 mL 6 x 50 mL

conicalHole angle ∠25° ∠25° ∠30° ∠45° ∠30° ∠30° ∠25°Rotor hole dimension (mm) 17 x 107.2 17 x 107.2 17.1 x 94 18 x 90

(Sleeve) 17.1 x 94 17.1 x 94 30x108.6

Rotor Hole Bottom type Conical Conical Round


(Sleeve)Round Round Conical

Max. height for tube fit (mm) 125 125 125


widercap)120 120 125

Max.Radius (mm) 103 103 100 175.2 103(I)/124(O) 103(I)/124(O) 98.4

1236R/1248R/1248


12,00016,582

4,0003,134

8,0007,370(I)/8,872(O)

10,00011,101

1580

1580R 15,00025,910

8,0007,370(I)/8,872(O)

13,00018,592

1696R 15,00025,910

15,00025,155

8,000(I)7,370(O)8,872


- I means Inner line, O means Outer line.- Maximum speed varies when using adapter.

1. Rotors


Rotor Shape

Cat. No. GRF-G-c15-12Not for 1248 GRF-B-c15-12 GRF-B-15-12 GRF-G-15-24A GRF-B-15-24 GRF-G-15-24

Not for 1248GRF-G-c50-6Not for 1248

Max. Capacity 12 x 15 mL 12 x 15 mL conical 12 x 15 mL 24 x 15 mL 24 x 15 mL 24 x 15 mL 6 x 50 mL

conicalHole angle ∠25° ∠25° ∠30° ∠45° ∠30° ∠30° ∠25°Rotor hole dimension (mm) 17 x 107.2 17 x 107.2 17.1 x 94 18 x 90

(Sleeve) 17.1 x 94 17.1 x 94 30x108.6

Rotor Hole Bottom type Conical Conical Round


(Sleeve)Round Round Conical



widercap)120 120 125

Max.Radius (mm) 103 103 100 175.2 103(I)/124(O) 103(I)/124(O) 98.4

1236R/1248R/1248


12,00016,582

4,0003,134

8,0007,370(I)/8,872(O)

10,00011,101

1580

1580R 15,00025,910

8,0007,370(I)/8,872(O)

13,00018,592

1696R 15,00025,910

15,00025,155

8,000(I)7,370(O)8,872

Rotor Shape

Cat. No. GRF-B-c50-6 GRF-G-50-8Not for 1248 GRF-B-50-8 GRF-G-85-6 GRF-B-85-6 GRF-G-250-6 GRF-B-250-6

Max. Capacity 6 x 50 mL conical 8 x 50 mL 8 x 50 mL 6 x 85 mL 6 x 85 mL 6 x 250/15 mL 6 x 250/15 mL

Hole angle ∠25° ∠30° ∠30° ∠34° ∠25° ∠25° ∠25°Rotor hole dimension (mm) 30 x 108.6 29.5 x 93.2 29.5 x 93.2 38.5 x 100 38.5 x 100 62x100/17x94

(250/15 mL)62x100/17x94(250/15 mL)

Rotor Hole Bottom type Conical Round Round Round Round Flat/Round

(250/15 mL)Flat/Round(250/15 mL)

Max. height for tube fit (mm) 125 120 120 122 125 130/120

(250/15mL)130/120

(250/15mL)

Max.Radius (mm) 98.4 103 103 110 110 134 134

1236R/1248R/1248


12,00016,582

10,00012,298

1580

1580R 15,00025,910

12,00017,709

8,0009,588

1696R 13,00018,592

15,00025,910

12,00017,709

8,0009,588

Rotor Shape

Cat. No. GRF-L-500-6 GRF-L-1000-4 GRF-L-1000-6

Max. Capacity 6 x 500 mL 4 x 1,000 mL 6 x 1,000 mL

Hole angle ∠25° ∠25° ∠25°Rotor hole dimension (mm) 70 x 137 98 x 144 98 x 144

Rotor Hole Bottom type Flat Flat Flat


Max.Radius (mm) 158 168.7 197.6

1736RMax. RPM(rpm)Max. RCF(xg)

8,00011,305 - -

2236R 10,00017,664

8,00012,071

7,00010,825


Applicable Adapters

Apdator

GAS-250(1000)

GAS-500(1000)

None

Tube capacity 250 mL 500 mL 1,000 mLAdapter hole dimension (Ф x L) 62 x 109 mm 69 x 149 mm -

Max.RPM/RCF 2236RGRF-L-1000-6 7,000rpm/8,990xg 7,000rpm/10,047xg 7,000rpm/10,825xgGRF-L-1000-4 8,000rpm/9,674xg 8,000rpm/11,055xg 8,000rpm/12,071xg

Adapter hole bottom type Flat -

Applicable Adapters

Apdator

GAS-250(500)

None

Tube capacity 250 1,000Adapter hole dimension (Ф x L) 69 x 100 -

Max.RPM/RCF(rpm/xg)1736R 8,000rpm/9,896xg 8,000rpm/11,305xg2236R 10,000rpm/15,462xg 10,000rpm/17,664xg

Adapter hole bottom type Flat -

25o

Max. capacity : 6 x 1,000 mLHole dimension: 98 x 144 mmMax. radius : 197.6 mmMax. height for tube fit: 175 mm

25o

Max. capacity: 6 x 500 mLHole dimension: 70 x 137 mmMax. radius: 158 mmMax. height for tube fit: 170 mm

25o

Max. capacity : 4 x 1,000 mLHole dimension: 98 x 144 mmMax. radius : 168.7 mmMax. height for tube fit: 175 mm

Fixed Angle RotorGRF-L-1000-6



1. Rotors

1.7 Adapters for Fixed-angle Rotors


Applicable Adapters

Apdator None None

Tube capacity 15 mL 250 mL

Max.RPM/RCF

1580R GRF-G-250-68,000rpm/9,395xg 8,000rpm/9,588xg

1696R GRF-B-250-61736R

GRF-L-250-610,000rpm/14,679xg 10,000rpm/14,981xg

2236R 13,000rpm/24,808xg 13,000rpm/25,318xg

Fixed Angle RotorGRF-G-250-6 / GRF-L-250-6 / GRF-B-250-6

1. Rotors


25o

Max. capacity : 6 x 250/15 mLHole dimension: (250mL) 62 x 100mm (15mL) 17 x 94mmMax. radius : 134 mmMax. height for tube fit: (250mL) 130mm (15mL) 120mm


Fixed Angle RotorGRF-G-85-6 / GRF-B-85-6 / GRF-L-85-6

Applicable Adapters

Apdator

GAS-15(85) GAS-c15(85) GAS-50(85)

Tube capacity 10 mL 15 mL 15 mL conicsl 50 mL Adapter hole dimension (Ф x L) 17 x 94 mm 17 x 98 mm 29 x 95 mm

Max.RPM/RCF

1580R GRF-G-85-612,000rpm/16,424xg 12,000rpm/16,582xg 12,000rpm/16,260xg 12,000rpm/16,743xg

1696R GRF-B-85-61236R/1248/1248R/1580 GRF-G-85-6 10,000rpm/11,404xg 10,000rpm/11,515xg 10,000rpm/11,292xg 10,000rpm/11,627xg

1736RGRF-L-85-6

15,000rpm/23,017xg 15,000rpm/23,268xg 15,000rpm/22,765xg 15,000rpm/23,520xg2236R 20,000rpm/40,919xg 20,000rpm/41,366xg 20,000rpm/40,472xg 20,000rpm/41,813xg

Adapter hole bottom type Round Conical Round

(G) 34o (B,L) : 25o

Max. capacity : 6 x 85 mLHole dimension: 38.5 x100 mm

Max. radius : (G,B) 110 mm (L) 99.5 mmMax. height for tube fit: (G)122mm (B,L)125mmSupplied with an O-ring

1. Rotors



Applicable Adapters

Apdator

GAS-c50(85)

None

Tube capacity 50 mL conical 85 mLAdapter hole dimension (Ф x L) 29.5 x 98 -

Max.RPM/RCF

1580R GRF-G-85-612,000rpm/16,421xg 12,000rpm/17,709xg

1696R GRF-B-85-61236R/1248/1248R/1580 GRF-G-85-6 10,000rpm/11,404xg 10,000rpm/12,298xg

1736RGRF-L-85-6

15,000rpm/23,017xg 15,000rpm/25,029xg2236R 20,000rpm/40,919xg 20,000rpm/44,496xg

Adapter hole bottom type Conical -

1. Rotors



Fixed Angle RotorGRF-G-50-8 / GRF-B-50-8 / GRF-L-50-8

Applicable Adapters

Apdator

GAS-15(50) GAS-c15(50)

None

Tube capacity 10 mL 15 mL 15 mL conical 50 mLAdapter hole dimension (Ф x L) 17 x 94 17 x 105 -

Max.RPM/RCF

2236R GRF-L-50-8 20,000rpm/41,366xg 20,000rpm/41,813xg 20,000rpm/42,931xg 20,000rpm/46,062xg1580R GRF-G-50-8

15,000rpm/23,268xg 15,000rpm/23,520xg 15,000rpm/24,149xg 15,000rpm/25,910xg1736R GRF-L-50-81696R GRF-B-50-81236R/1248R/1580 GRF-G-50-8 12,000rpm/14,892xg 12,000rpm/15,053xg 12,000rpm/15,455xg 12,000rpm/16,582xg

Adapter hole bottom type Round Conical -

30o Max. capacity : 8 x 50 mLHole dimension: 29.5 x 93.2 mmMax. radius : 103 mmMax. height for tube fit: 120 mmSupplied with an O-ring

1. Rotors



Fixed Angle RotorGRF-L-50-6 / GRF-SR-50-6

Applicable Adapters

Apdator

GAS-15(50) GAS-c15(50)

None

Tube capacity 10 mL 15 mL 15 mL 50 mLAdapter hole dimension (Ф x L) 17 x 94 17 x 105 -

Max.RPM/RCF2236R

GRF-L-50-6N/A N/A N/A 22,000rpm/54,111xg

1736R N/A N/A N/A 17,000rpm/32,310xg624R GRF-SR-50-6 6,000rpm/3,538xg 6,000rpm/3,578xg 6,000rpm/3,663xg 6,000rpm/3,824xg

Adapter hole bottom type Round Conical -

(L)30o (SR)25o

Max. capacity : 6 x 50 mLHole dimension: (L)29.5 x 92.2 mm (SR) 29.5 x 95.4 mmMax. radius : (L)100 mm (SR)95 mmMax. height for tube fit: 120 mmSupplied with an O-ring

1. Rotors



Fixed Angle RotorGRF-G-c50-6 / GRF-B-c50-6 / GRF-L-c50-6 / GRF-SR-c50-6

Applicable Adapters

Apdator

GAS-c15(c50)

None

Tube capacity 15 mL conical 50 mL conicalAdapter hole dimension (Ф x L) 17 x 105 -

Max.RPM/RCF

1580R GRF-G-c50-613,000rpm/17,685xg 13,000rpm/18,592xg

1696R GRF-B-c50-61236R/1248R1580 GRF-G-c50-6 10,000rpm/10,464xg 10,000rpm/11,101xg

1736RGRF-L-c50-6

15,000rpm/23,545xg 15,000rpm/24,753xg2236R 17,000rpm/30,242xg 17,000rpm/31,793xg

624R GRF-SR-c50-6 6,000rpm/3,767xg 6,000rpm/3,960xg

Adapter hole bottom type Conical -

25o Max. capacity : 6 x 50 mL conicalHole dimension: 30 x 108.6 mmMax. radius : 98.4 mmMax. height for tube fit: 125 mmSupplied with an O-ring

1. Rotors



Fixed Angle RotorGRF-L-15-12 / GRF-B-15-12

Fixed Angle RotorGRF-G-15-24 / GRF-B-15-24

Applicable Adapters

Apdator None None

Tube capacity 10 mL 15 mL

Max.RPM/RCF2236R

GRF-L-15-1220,000rpm/44,720xg

1736R 17,000rpm/32,310xg1696R GRF-B-15-12 15,000rpm/25,155xg

30o

Max. capacity : 12 x 15 mLHole dimension: 17 x 93.2 mmMax. radius : 100 mmMax. height for tube fit: 125 mmSupplied with an O-ring

Applicable Adapters

Apdator None

Tube capacity 15 mL

Max.RPM/RCF1248R/1580R1236R GRF-G-15-24 8,000rpm

(I) 7,370xg (O) 8,872xg1696R GRF-B-15-12

30o Max. capacity : 24 x 15 mLHole dimension: 17.1 x 94 mmMax. radius : Inner holes: 103 / Outer holes: 124mmMax. height for tube fit: 120 mmSupplied with an O-ring

(I) - Inner holes (O) Outer holes

1. Rotors



Fixed Angle RotorGRA-G-15-24A

Fixed Angle RotorGRA-SR-15-24

SleeveGLB-15A-FA

SleeveGLB-15A-FA

45o Max. capacity : 24 x 15 mLHole diameter: 18 mmMax. radius : 175.2 mmMax. height for tube fit: 125 mm (120 for conical/wider cap)

45o Max. capacity : 24 x 15 mLHole diameter: 18 mmMax. radius :(I)138mm (O)155 mmMax. height for tube fit: 125 mm (120 for conical/wider cap)

Hole dimension(øxL): 18 x 90 mmHole bottom type: Flat bottom with rubber pad


Applicable Adapters

Apdator

GAS-c5(15) GAS-3(15) GAS-15(15)None None None

Tube capacity 5 mL conical 3 - 4 mL 4 - 7 mL 10 mL 15 mL 15 mL conicalAdapter hole dimension (Ф x L) 14x20 13.5 x 61 13.5 x 85 - - -

Max.RPM/RCF

1236R1248/1248R1580/1580R

GRA-G-15-24A 4,000rpm/2,503xg 4,000rpm/2,776xg 4,000rpm/2,990xg 4,000rpm/3,121xg 4,000rpm/3,134xg 4,000rpm/3,068xg

624R GRA-SR-15-24 4,000rpm(I)1,884/(O)2,170xg

4,000rpm(I)2,080/(O)2,367xg

4,000rpm(I)2,447/(O)2,733xg

4,000rpm(I)2,460/(O)2,764xg

4,000rpm(I)2,469/(O)2,773xg

4,000rpm(I)2,379/(O)2,665xg

Adapter hole bottom type Conical Round - - -

1. Rotors


(I) - Inner holes (O) Outer holes




Fixed Angle RotorGRA-S-15-16A

Fixed Angle RotorGRA-15/10-12

SleeveGLB-15A

SleeveGLB-15/10A

45o

Max. capacity : 16 x 15 mLHole diameter: 18 mmMax. radius : 150.9 mmMax. height for tube fit: 125 mm (120 for conical/wider cap)

45o

Max. capacity : 6 x 15 mL / 12 x 10 mLHole diameter: 18 mmMax. Radius : 116 mmMax. height for tube fit: 125 mm (120 for conical tube)

Applicable Adapters

Apdator

GAS-c5(15) GAS-3(15) GAS-5(15)None None None

Tube capacity 5 mL conical 3 - 4 mL 4 - 7 mL 10 mL 15 mL 15 mL conical Adapter hole dimension (Ф x L) 14x20 13.5 x 61 13.5 x 85 - - -Max.RPM/RCF 416 GRA-S-15-16A 4,000rpm/2,120xg 4,000rpm/2,397xg 4,000rpm/2,612xg 4,000rpm/2,692xg 4,000rpm/2,700xg 4,000rpm/2,638xg


Applicable Adapters

Apdator

GAS-3(15) GAS-3(15) GAS-5(15)None None None

Tube capacity 5 mL conical 3 - 4 mL 4 - 7 mL 10 mL 15 mL 15 mL conical Adapter hole dimension (Ф x L) 14 x 20 13.5 x 61 13.5 x 85 - - -Max.RPM/RCF 406 GRA-15/10-12 4,000rpm/1,458xg 4,000rpm/1,762xg 4,000rpm/1,986xg 4,000rpm/2,057xg 4,000rpm/2,075xg 4,000rpm/2,003xg


1. Rotors



Fixed Angle RotorGRA-G-c15-12 / GRA-B-c15-12 / GRA-L-c15-12

25o

Max. capacity : 12 x 15 mL conicalHole dimension: 17 x 107.2 mmMax. radius : 103 mmMax. height for tube fit: 125 mm Supplied with an O-ring

Applicable AdaptersAdapter NoneTube capacity 15 mL conical

Max.RPM/RCF

1236R1248R/1580 GRA-G-c15-12

12,000rrpm/16,582xg

1580R 15,000rrpm/25,910xg1736R GRA-L-c15-12

15,000rrpm/25,910xg1696R GRA-B-c15-12

2236R GRA-L-c15-12 20,000rrpm/46,062xg

1. Rotors

1.7 Adapter for Fixed-angle Rotors

Sleeve hole dimension(øxL): 18 x 90 mmHole bottom type: Flat bottom with rubber pad

Fixed Angle RotorGRA-15-6A

SleeveGLB-15A

45o

Max. capacity : 6 x 15 mLHole dimension: 18 x ??? mmMax. radius : 116 mmMax. height for tube fit: 125 mm (120 for conical tube)

Applicable Adapters

Apdator

GAS-3(15) GAS-3(15) GAS-5(15)None None None

Tube capacity 5 mL conical 3 - 4 mL 4 - 7 mL 10 mL 15 mL 15 mL conical Adapter hole dimension (Ф x L) 14x20 13.5 x 61 13.5 x 85 - - -Max.RPM/RCF 406 GRA-15-6A 4,000rpm/1,458xg 4,000rpm/1,762xg 4,000rpm/1,986xg 4,000rpm/2,057xg 4,000rpm/2,075xg 4,000rpm/2,003xg





1. Rotors

1.8 Tube Recommendation for Fixed-angle Rotors

Rotor AdapterHole diameter

Rotor(Ø)Adapter(Øxh)

Tube Capacity Tube Tube

Material Max. g-force(xg) Tube dimension O.D. x h

GRF-G-15-24AGRF-SR-15-24AGRA-S-15-16A

GAS-3(15) 13.5 x 61mm 3~4mLblood tube

Vacutainer 13 x 75 mmN/A N/A 13 x 75 mmVACUETTE 13 x 75 mm

AMPULAB 13 x 75 mm

GAS-5(15) 13.5 x 65mm5~7mL bloodtube


AMPULAB 13 x 100 mm

GAS-c5(15) 14 x 20mm 5 mLconical

Biosigma(Snap Cap) PP 21,000 xg 17 x 60mmEppendorf(Snap Cap) PP 25,000 xg 17 x 60mm

SPL PP 25,000 xg 17 x 62mm

-

18mm 8~10mL blood tube


AMPULAB 16 x 100 mm18 x 90mm 15mL Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8

18 x 90mm 15 mLconical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120

Eppendorf 0030122151 19,500 xg 17 x 121

Orange 4440300N 10,000 xg 17 x 121

GRA-15-6AGRA-15/10-12

GAS-3(15) 13.5x61mm 3~4mLblood tube


AMPULAB 13 x 75 mm



AMPULAB 13 x 100 mm


Biosigma(Snap Cap) PP 21,000 xg 17 x 60mm

Eppendorf(Snap Cap) PP 25,000 xg 17 x 60mm

SPL PP 25,000 xg 17 x 62mm

-



AMPULAB 16 x 100 mm

18 x 90mm 15mL Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8


Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120

Eppendorf 0030122151 19,500 xg 17 x 121Orange 4440300N 10,000 xg 17 x 121

▶ Microtubes are excluded.▶ Recommended microplates information is currently being updated.▶ These tube specifications above are retrieved from tube manufacturers information documents.

PP - PolypropylenePPCO - Polypropylene Copolymer

① Use with friction fit closures (Cat. No. DS3111-series) for high speed centrifugation.





Material Max. g-force(xg) Tube dimension O.D. x h

GRF-G-15-24AGRF-SR-15-24AGRA-S-15-16A

GAS-3(15) 13.5 x 61mm 3~4mLblood tube


AMPULAB 13 x 75 mm



AMPULAB 13 x 100 mm


Biosigma(Snap Cap) PP 21,000 xg 17 x 60mmEppendorf(Snap Cap) PP 25,000 xg 17 x 60mm

SPL PP 25,000 xg 17 x 62mm

-



AMPULAB 16 x 100 mm18 x 90mm 15mL Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8


Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120

Eppendorf 0030122151 19,500 xg 17 x 121

Orange 4440300N 10,000 xg 17 x 121

GRA-15-6AGRA-15/10-12

GAS-3(15) 13.5x61mm 3~4mLblood tube


AMPULAB 13 x 75 mm



AMPULAB 13 x 100 mm


Biosigma(Snap Cap) PP 21,000 xg 17 x 60mm


SPL PP 25,000 xg 17 x 62mm

-



AMPULAB 16 x 100 mm

18 x 90mm 15mL Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8


Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120



PP - PolypropylenePPCO - Polypropylene CopolymerPC - Polycarbonates FEP - Fluorinated ethylene propylene

1. Rotors





Material Max. g-force(xg) Tube dimension O.D. x h, mm

GRF-L-15-12 - 17mm 15mL Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8GRF-B-15-12 - 17mm 15mL Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8GRF-B-15-24GRF-G-15-24 - 17.5mm 15mL Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8

GRF-L-c15-12GRF-B-c15-12GRF-L-c15-12

- 17mm 15 mLconical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120


GRF-L-50-6GRF-SR-50-6GRF-L-50-8GRF-G-50-8GRF-B-50-8

GAS-15(50) 17 X 94mm12 mL

Nalgene 3110-0120 ① PPCO 50,000 xg 15.8 x 102.1 Nalgene 3117-0120 ① PC 50,000 xg 15.9 x 103.1

10 mL Nalgene 3114-0010 FEP 50,000 xg 16.0 x 79.015 mL Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8

GAS-c15(50) 17 X 105mm 15 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120


- 29.5mm 50 mL Nalgene 3119-0050 ② PPCO 100,605 xg 28.8 x 105.9

GRF-L-c50-6GRF-G-c50-6GRF-B-c50-6GRF-SR-c50-6

GAS-c15(c50) 17 x 94mm 15 mLconical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120


- 29.5mm 50 mLconical

Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115

Eppendorf 0030122178 19,500 xg 30 x 116.2Corning 4558 15,500 xg 29.1 x 114.4

① Use with friction fit closures (Cat. No. DS3111-series) for high speed centrifugation.② For leakproof performance >10,000 xg, use a sealing closure DS3131-0024.







GAS-15(85) 17 x 94mm

12 mLNalgene 3110-0120 ① PPCO 50,000 xg 15.8 x 102.1

Nalgene 3117-0120 ① PC 50,000 xg 15.9 x 103.1

10 mL Nalgene 3114-0010 FEP 50,000 xg 16.0 x 79.0

15 mL Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8

GAS-c15(85) 17 x 98mm 15 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5

Falcon 352096 12,000 xg 17 x 120

Eppendorf 0030122151 19,500 xg 17 x 121

Orange 4440300N 10,000 xg 17 x 121

GAS-50(85) 29.5mm 50 mL Nalgene 3119-0050 ② PPCO 100,605 xg 28.8 x 105.9

GAS-c50(85) 29.5mm 50 mLconical

Orange 4440100N

PP

12,000 xg 30 x 114

Falcon 352070 16,000 xg 30 x 115

Eppendorf 0030122178 19,500 xg 30 x 116.2

Corning 4558 15,500 xg 29.1 x 114.4

- L: 38.3mmG/B: 38.5mm 85 mL Nalgene 3118-0085 PC 50,000 xg 38.2 x 105.2

GRF-L-250-6GRF-G-250-6GRF-B-250-6

-

17mm 15 mL Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8

62mm 250 mL

Nalgene 3120-0250 ③ PPCO 13,200 xg 61.8 x 125.2

Nalgene 3122-0250 ③ PC 27,500 xg 61.8 x 125.2

Autofill 1801-RLS ④ PP 8,250 59 x 129

Autofill 1803-RLS ⑤ PP 27,500 59 x 129

Autofill 1802-RLS ④ PC 8,250 59 x 129

Autofill 1804-RLS ⑤ PC 31,700 59 x 129

1. Rotors


① Use with friction fit closures (Cat. No. DS3111-series) for high speed centrifugation.② For leakproof performance >10,000 xg, use a sealing closure DS3131-0024.③ To assure leakproof, especially at higher speeds and fill volumes, use sealing closure DS3131-0038.④ Bottle with screw cap for low speeds. ⑤ Bottle with leak-proof sealing cap for high speeds.








GRF-L-500-6

GAS-250(500) 69 x 100mm 250 mL

Nalgene 3120-0250 ① PPCO 13,200 xg 61.8 x 125.2Nalgene 3122-0250 ① PC 27,500 xg 61.8 x 125.2Autofill 1801-RLS ⑤ PP 8,250 59 x 129 Autofill 1803-RLS ⑥ PP 27,500 59 x 129 Autofill 1802-RLS⑤ PC 8,250 59 x 129 Autofill 1804-RLS ⑥ PC 31,700 59 x 129

- 70mm 500 mL

Nalgene 3120-9500 PPCO 13,700 xg 69.5 x 158.9 Nalgene 3122-0500 PC 13,700 xg 69.5 x 158.9Autofill 1805-RLS② PP 8,250 xg 68 x 196Autofill 1807-RLS ③ PP 13,500 xg 68 x 196Autofill 1806-RLS ② PC 8,250 xg 68 x 196Autofill 1808-RLS ③ PC 13,850 xg 68 x 196

GRF-L-1000-4GRF-L-1000-6

GAS-250(1000) 62 x 109mm 250 mL

Nalgene 3120-0250 ① PPCO 13,200 xg 61.8 x 125.2Nalgene 3122-0250 ① PC 27,500 xg 61.8 x 125.2Autofill 1801-RLS ⑤ PP 8,250 59 x 129 Autofill 1803-RLS ⑥ PP 27,500 59 x 129 Autofill 1802-RLS⑤ PC 8,250 59 x 129 Autofill 1804-RLS ⑥ PC 31,700 59 x 129

GAS-500(1000) 69 x 149mm 500 mL

Nalgene 3120-9500 PPCO 13,700 xg 69.5 x 158.9 Nalgene 3122-0500 PC 13,700 xg 69.5 x 158.9Autofill 1805-RLS② PP 8,250 xg 68 x 196Autofill 1807-RLS ③ PP 13,500 xg 68 x 196Autofill 1806-RLS ② PC 8,250 xg 68 x 196Autofill 1808-RLS ③ PC 13,850 xg 68 x 196

- 98mm 1,000 mL Nalgene 3120-1000 ④ PPCO 7,100 98.0 x 174.2

1. Rotors


① To assure leakproof, especially at higher speeds and fill volumes, use sealing closure DS3131-0038.② Bottle with screw cap for low speeds. ③ Bottle with leak-proof sealing cap for high speeds.④ To assure leakproof, especially at higher speeds and fill volumes, use sealing closure DS3132-0063.⑤ Bottle with screw cap for low speeds. ⑥ Bottle with leak-proof sealing cap for high speeds.





1. Rotors

1.9 Swing-out Rotor Chart (RPM and Capacity)

3000

2000

1000

500

200

100

MTP

3.5K 4K 4.5K 5K

Roto

r tot

al ca

paci

tymL

3.5 K 4K 4.5K 5 K

GRS-L-r250-4GRS-G-r250-4GRS-B-r250-4

GRS-G-r750-4GRS-B-r750-4

GRS-G-750-4 GRS-B-750-4GRS-B-1000-4

4000

GRS-G-100-6GRS-B-100-6

GRS-S-15-6

GLP-S(SR)-mw-2

GRS-SR-15-6

GRS-G-250-4GRS-B-250-4

GRS-SR-50-4GRS-S-50-4

GRS-SR-100-4GRS-S-100-4

Max. RPM


1. Rotors

1.10 Swing-out Rotors Specifications

-Large Capacity, High Speed Centrifuges-

-Multi Purpose High Speed Centrifuges-

-Low Speed Clnical Centrifuges-

Rotor Shape

Cat. No. GRS-L-r250-4

Aangle ∠90°

Max. Capacity 4 x 250 mL

1736R, 2236R Max.RPM/RCF 4,000/3,134

Rotor Shape

Cat. No. GRS-G-100-6GRS-B-100-6

GRS-G-250-4GRS-B-250-4



GRS-G-750-4GRS-B-750-4 GRS-B-1000-4

Angle ∠90° ∠90° ∠90° ∠90° ∠90° ∠90°

Max. Capacity 6 x 100 mL 4 x 250 mL 4 x 250 mL 4 x 500 mL 4 x 750 mL 4 x 1,000 mL

1236R

Max.RPM/RCF

(G)4,000/3,105

5,000/5,394 4,000/3,134

1248R

1248

1580

3,600/3,159

(G)4,000/3,900

1580R

1696R (B)4,200/3,424

(B)4,600/5,157 3,700/,3337

Rotor Shape

Cat. No. GRS-S-15-6GRS-SR -15-6

GRS-S-50-4GRS-SR-50-4

GRS-S-100-4GRS-SR-100-4

GRS-S-mw-2GRS-SR-mw-2

Angle ∠90° ∠90° ∠90° ∠90°

Max. Capacity 6 x 15 mL 4 x 50 mL 4 x 100 mL 4 x MTP

416

Max.RPM/RCF

(S)4,000/2,826

(S)4,000/2,700

(S)4,000/2,774

(S)4,000/2,000

624R (SR)4,400/3,420

(SR)4,800/3,888

(SR)4,200/3,059

(SR)4,000/2,000


Applicable Adapters

Apdater

GAM-10-5(r1000)

GAM-15-28(r1000)

GAM-c15-22(r1000)

GAM-50-11(r1000)

GAM-c50-10(r1000)

GLP-mw-r1000

Tube 10 mL 15 mL 15 mL conical 50 mL 50 mL

conical MTP DWP

Rack capacity(ea/4) 35/140 28/112 22/88 11/44 10/40 6/24 2/8

Hole dimension(Ф x L) 16 x 85 mm 17.2 x 85 mm 17 x 90 mm 29.2 x 87 mm 29.5 x 92.5 mm 130 x 88.5 mm

Hole bottom type Round Round Conical Round Conical Flat

Hole dimension (w x d x h) : 97 x 134 x 94 mmMax. RPM/RCF : 3,700 rpm / 3,084 xgRadius: 201.5 mmHole bottom type : FlatMax. height for tube fit: 120(w/cap) 130(w/o cap) mm

1.11 Buckets for Swing-out Rotors

1. Rotors

Rotor GRS-B-1000-4 : 1696RBucket GLB-r1000-1000


1

1

2

2

3

3

4

4

5

5

6

6

A A

B B

C C

D D

User 2019-03-08

설계자 점검자 승인자 날짜

1 / 1 에디션 용지

날짜

Applicable Adapters

Apdater

GAM-5-48(h1000)

GAM-10-38(h1000)

GAM-15-29(h1000)

GAM-c15-29(h1000)

GAM-50-13(h1000)

GAM-c50-13(h1000)

GAM-250-2(h1000)

GLP-mw-r1000

Tube 3 mL 5 mL 10 mL 15 mL 15mL conical 50mL 50mL conical 250mL MTP DWP

Rack capacity(ea/4) 48/192 48/192 38/152 29/116 29/116 13/52 13/52 2/8 6/24 2/8

Hole dimension(Ф x L, mm) 13.2 x 50 13.2 x 85 16 x 85 17.2 x 85 17 x 90 29.2 x 87 29.5 x 92.5 62 x 87 130 x 88.5

Hole bottom type Round Round Round Round Conical Round Conical Flat Flat

Hole dimension (w x d x h) : 110 x 168.7 x 95 mmMax. RPM/RCF : 3,700 rpm / 3,092 xgRadius: 202 mmHole bottom type : FlatMax. height for tube fit: 130 mm


1. Rotors

Bucket GLB-h1000-1000

Rotor GRS-B-1000-4 : 1696R


Applicable Adapters

Apdater

GAM-250(750) GAM-c250(750)

GAM-c500(750)

GAM-500(750)

None None

Tube capacity 250 mL 250 mL conical

500 mLconical 500 mL 750 mL 1,000 mL

Rack capacity(ea/4) 1/4 1/4 1/4 1/4 1/4 1/4

Hole dimension(Ф x L) 62.3 x 87 mm 60.5 x 125 mm 95.6 x 58 mm 75.5 x 98.7 99 x 114 mm

(Bucket)99 x 114 mm

(Bucket)Hole bottom type Flat Conical Conical Flat Flat Flat

Hole dimension (w x d x h) : 99 x 120 mmMax. RPM/RCF : 3,700 rpm / 3,337 xgRadius: 218 mmHole bottom type : FlatMax. height for tube fit: 143(w/ cap)/170(w/o cap) mm

Applicable Adapters

Apdater

GAM-5-24(750)

GAM-10-21(750)

GAM-15-19(750)

GAM-c15-14(750)

GAM-50-7(750)

GAM-c50-5(750)

Tube capacity 3 mL 5 mL 10 mL 15 mL 15 mL conical 50 mL 50 mL conical

Rack capacity(ea/4) 24/96 21/84 19/76 14/56 7/28 5/20

Hole dimension(Ф x L) 13.2 x 58 mm 13.2 x 85 mm 16 x 85 mm 17.2 x 85 mm 17 x 85 mm 29.2 x 87 mm 29.2 x 88 mm

Hole bottom type Round Round Round Conical Round Conical

Rotor GRS-B-1000-4 : 1696R

Bucket GLB-b1000-1000


1. Rotors



1. Rotors



Bucket GLB-b750-750/ GLB-750-750 (For GRS-G-750-4)

Rotor GRS-G-750-4 / GRS-B-750-4

Applicable Adapters

Apdater

GAM-5-24(750)

GAM-10-21(750)

GAM-15-19(750)

GAM-c15-14(750)

GAM-50-7(750)

GAM-c50-5(750)

Tube capacity 3 mL 5 mL 10 mL 15 mL 15 mL conical 50 mL 50 mL conicalRack capacity(ea/4) 24/96 21/84 19/76 14/56 7/28 5/20Hole dimension(Ф x L) 13.2 x 58 mm 13.2 x 85 mm 16 x 85 mm 17.2 x 85 mm 17 x 85 mm 29.2 x 87 mm 29.2 x 88 mm

Hole bottom type Round Round Round Conical Round Conical

Hole dimension (∅ x L) : 99 x 105 mmMax. RPM/RCF : 4,600 rpm / 4,850 xgRadius: 205 mmBucket type : FlatMax. height for tube fit: 130(w/ cap)/142(w/o cap)

Hole dimension (∅ x L) : 99 x 101.5 mmMax. RPM/RCF : 4,000 rpm / 3,667 xgRadius: 205 mmBucket type : FlatMax. height for tube fit: 140(w/ cap)/160(w/o cap)

Bucket GLB-b750-750B(For GRS-B-750-4)

-G- : 1580, 1580R -B- : 1696R

Applicable Adapters

Apdater

GAM-250(750) GAM-c250(750) GAM-c500(750) GAM-500(750)

None

Tube capacity 250 mL 250 mL conical 500 mL conical 500 mL 750 mLRack capacity(ea/4) 1/4 1/4 1/4 1/4 1/4Hole dimension(Ф x L) 62.3 x 87 mm 60.5 x 125 mm 95.6 x 58 mm 75.5 x 98.7 mm 99 x 114mm (Bucket)

Hole bottom type Flat Conical Conical Flat Flat


1. Rotors


Applicable Adapters

Apdater None

Tube capacity 500 mLBucket

capacity(ea/4) 1/4

Hole dimension (∅ x L) : 70 x 135 mmMax. RPM/RCF : (GLB-500-750)4,000 rpm / 3,900 xg (GLB-500-750B)4,600 rpm / 5,157 xgRadius: 218 mmBucket type : FlatMax. height for tube fit: 165 mm

GLB-500-750 (For GRS-G-750-4) GLB-500-750B (For GRS-B-750-4)

Applicable Adapters

Apdater None

Tube capacity MTP DWPBucket

capacity(ea/4) 4/16 1/4

Holder dimension (∅ x L) : 88 x 128.5 x 80mmMax. RPM/RCF : 4,000rpm / 3,068xgRadius: 171.5 mmBottom type : Flat

Hole dimension (W x D x L) : 93 x 130 x 63 mmMax. RPM/RCF : 4,600rpm / 4,033xgRadius: 170.5 mmBucket type : FlatMax. height for tube fit : 63mm

GLP-mw-750 (For GRS-G-750-4) GLP-mw-750B (For GRS-B-750-4)


1. Rotors

Rotor GRS-G-750-4 / GRS-B-750-4 -G- : 1580, 1580R -B- : 1696R


Rotor GRS-G-r750-4 / GRS-B-r750-4

Applicable Adapters

Apdater

GAM-5-30(r750)

GAM-15-20(r750)

GAM-c15-16(r750)

GAM-50-8(r750)

GAM-c50-5(r750)

GAM-250(r750)

Tube capacity 3mL 5mL 10/15mL 15mL conical 50 mL 50 mL conical 250 mLRack capacity(ea/4) 30/120 20/80 16/64 8/32 5/20 1/4Hole dimension(Ф x L) 13 x 56.5 mm 13 x 85 mm 17.2 x 85 mm 17.5 x 85 mm 29.5 x 85 mm 29.5 x 85 mm 62.5 x 87 mm

Hole bottom type Round Round Conical Round Conical Flat

Hole dimension (W x D x H) : 99 x 99 x 98 mmMax. RPM/RCF : 3,600 rpm / 2,898 xgRadius: 200 mmBucket type : FlatMax. height for tube fit: 150 mm

Bucket GLB-r750-r750

-G- : 1580, 1580R -B- : 1696R

Applicable Adapters

Apdater

GAM-c250(r750)

GAM-c500(r750)

GAM-c500(r750)

Tube capacity 250 mL conical 500 mL conical 500 mLRack capacity(ea/4) 1/4 1/4 1/4Hole dimension(Ф x L) 61 x 93 mm 98 x 94 mm 74.1 x 92.5 mm

Hole bottom type Conical Conical Flat


1. Rotors


Applicable Adapters

Apdater None


capacity(ea/4) 1/4

Hole bottom type Flat

Hole dimension (∅ x L) : 70 x 135 mmMax. RPM/RCF : 3,600rpm / 3,159xgRadius: 218 mmBucket type : FlatMax. height for tube fit: 165 mm

Bucket GLB-500-r750

Applicable Adapters

Apdater None



Hole bottom type Flat

Bucket bore (W x D x L) : 88 x 128.5 x 80mmMax. RPM/RCF : 3,600rpm / 2,485xgRadius: 171.5 mmBucket type : Flat

Bucket GLP-mw-750


1. Rotors

Rotor GRS-G-r750-4 / GRS-B-r750-4 -G- : 1580, 1580R -B- : 1696R


Rotor GRS-G-r250-4 / GRS-L-r250-4 / GRS-B-r250-4

Applicable Adapters

Apdater

GAM-m2.0-20(r250)

GAM-5-12(r250)

GAM-15-12(r250)

GAM-c15-9(r250)

GAM-50-4(r250)

GAM-c50-3(r250)

Tube capacity 1.5/2.0 mL 3 mL 5 mL 15 mL 15 mL Conical 50 mL 50 mL conicalRack capacity(ea/4) 20 / 80 12 / 48 12 / 48 9 / 36 4 / 16 3 / 12Hole dimension(Ф x L) 11.3 x 39 mm 13.5 x 58 mm 13.5 x 80mm 17.5 x 90 mm 17.5 x 90 30.2 x 90 30.5 x 90

Bottom type Round Round Round Round Conical Round Conical

Hole dimension (W x D x H) : 86 x 70.3 x 102.5 mmMax. RPM/RCF : 4,000 rpm / 3,134 xgRadius : 175.2 mmBottom type : FlatMax. height for tube fit : 130 mm


Applicable Adapters

Apdater

GAM-s50-4(r250)

GAM-85-2(r250)

GAM-250(r250)

Tube capacity 50 mL syringe 15 mL 85 mL 250 mLRack capacity(ea/4) 4 / 16 2 / 8 1 / 4Hole dimension(Ф x L) 31.4 x 65 17 x 86.5 38.5 x 86.5 62.5 x 87

Bottom type Flat Round Flat

-G- : 1236R/1248R/1248/1580/1580R -L- : 1736R/2236R

-B- : 1696R


1. Rotors



Applicable Adapters

Apdater None



Hole dimension (W x D x H) : 88 x 128.5 x 80mmMax. RPM/RCF : 4,000rpm / 2,737xgRadius: 153 mmBottom type : Flat

Bucket GLP-mw-r250

-G- : 1236R/1248R/1248/1580/1580R -L- : 1736R/2236R

-B- : 1696R


1. Rotors



Rotor GRS-G-250-4 / GRS-B-250-4

Applicable Adapters

Apdater None

Tube capacity 250mLBucket

capacity(ea/4) 1 / 4

Applicable Adapters

Apdater None

Tube capacity 250mL conicalBucket


Hole dimension (ø x L) : 62 x 109 mmMax. RPM/RCF : 5,000 rpm / 5,394 xgRadius: 193 mmBottom type : FlatMax. height for tube fit : 153 mm

Bucket bore (ø x L) : 60.5 x 130.2 mmMax. RPM/RCF : 5,000 rpm / 5,394 xgRadius: 193 mmBottom type : ConicalMax. height for tube fit : 153 mm

Bucket GLB-250-250

Bucket GLB-c250-250

-G- : 1236R/1248R/1248/1580/1580R -B- : 1696R


1. Rotors


Bucket bore (ø x L) : 22.5 x 100(20)/16 x 90(10) mmMax. RPM/RCF : 5,000 rpm / 4,752(20)4,472(10) xgRadius: 170(20), 160(10) mmBottom type : FlatMax. height for tube fit : 132(20) / 125(10) mm

Applicable Adapters

Apdater

GAS-60s(60)

Tube capacity 60mL syringeRack capacity(ea/4) 1 / 4

Hole dimension Custom-made

Applicable Adapters

Apdater

GAS-10s(10) GAS-20s(20)

Tube capacity 10 mL syringe 20 mL syringeRack capacity(ea/4) 2 / 8 2 / 8

Hole dimension Custom-made Custom-made

Hole dimension (ø x L) : 31.5 x 130.2 mmMax. RPM/RCF : 5,000 rpm / 5,394 xgRadius: 193 mmBottom type : FlatMax. height for tube fit : 165 mm

Bucket GLB-60-250

Bucket GLB-d20/10-250


1. Rotors

Rotor GRS-G-250-4 / GRS-B-250-4 -G- : 1236R/1248R/1248/1580/1580R -B- : 1696R


Rotor GRS-G-100-6 / GRS-B-100-6 / GRS-S-100-4 / GRS-SR-100-4

Applicable Adapters

Apdater

GAM-m2.0-6(b100)

GAM-7-5(b100)

GAM-10-5(b100)

GAM-15-3(b100)

GAM-c15-3(b100)

GAM-c15(b100)

Tube capacity 1.5-2.0 mL 2.6-7 mL 10 mL 9-15 mL 15 mL conical 15 mL conicalRack capacity

for 4place rotor(ea/4)for 6place rotor (ea/6)

6 / 246 / 36

5 / 205 / 30

5 / 205 / 30

3 / 123 / 18

3 / 123 / 18

1 / 41 / 6

Hole dimension(Ф x L) 11 x 39 13.5 x 60 16 x 60 17.5 x 105 17.2 x 106.5 17.2 x 106.5Bottom type Conical Round Round Round Conical Conical

Hole dimension (ø x L) : 47 x 99 mmMax. RPM/RCF : (G)4,000 rpm / 3,105 xg (B)4,200 rpm / 3,424 xg (S)4,000 rpm / 2,700 xg (SR)4,200 rpm / 3,059 xgRadius: (G),(B)173.6mm (S),(SR)155.1mmHole bottom type : FlatMax. height for tube fit : 120(w/ cap)/130(w/o cap)

Supplied with an O-ringBucket GLB-b100-100

Applicable Adapters

Apdater

GAM-50(b100)

GAM-c50(b100)

GAM-85(b100)

GAM-100(b100)

Tube capacity 50 mL 50 mL conical 85mL 100 mLRackcapacity

for 4place rotor (ea/4)for 6place rotor (ea/6)

1 / 41 / 6

1 / 41 / 6

1 / 41 / 6

1 / 41 / 6

Hole dimension(Ф x L) 29.5 x 95.9 30 x 100 38.5 x 106.4 44.2 x 93Bottom type Round Conical Round Round

-G- : 1236R/1248R/1248/1580/1580R

-S- : 416-SR- : 624R

-B- : 1696R


1. Rotors


Applicable Adapters

Apdater

GAS-u15(50) GAS-c50(50)

None

Tube capacity 15 mL 15 mL conical 50 mL conical 50 mLRack capacity


1 / 41 / 6

1 / 41 / 6

1 / 41 / 6

1 / 41 / 6

Hole dimension(Ф x L) 17.3 x 87 29.5 x 17.5 -Bottom type Open Conical -

Supplied with an O-ringHole dimension (ø x L) : 30.5 x 97 mmMax. RPM/RCF : (G)4,000 rpm / 3,032 xg (B)4,200 rpm / 3,343 xg (S)4,000 rpm / 2,700 xg (SR)4,200 rpm / 2,976 xgRadius: (G)169.5mm (B)169.5mm (S),(SR)150.9mmHole bottom type : FlatMax. height for tube fit : 115(w/ cap)/125(w/o cap)

Bucket GLB-b50-100

Applicable Adapters

Apdater None

Tube capacity 50 mL conicalBucketcapacity


1 / 41 / 6

Hole dimension (ø x L) : 30 x 89 mmMax. RPM/RCF : (G)4,000 rpm / 3,105 xg (B)4,200 rpm / 3,424 xg (S)4,000 rpm / 2,774 xg (SR)4,200 rpm / 3,059 xgRadius: (G),(B)173.6mm (S),(SR)155.1mmHole bottom type : FlatMax. height for tube fit : 125 mm

Bucket GLB-dc50-100

-G- : 1236R/1248R/1248/1580/1580R

-S- : 416-SR- : 624R

-B- : 1696R


1. Rotors




Applicable Adapters

Apdater None

Tube capacity 15 mL roundBucketcapacity

for 4place rotor (ea/4) for 6place rotor (ea/6)

2 / 4 2 / 12

Applicable Adapters

Apdater None

Tube capacity 15 mL conicalBucketcapacity


2 / 8 2 / 12

Hole dimension (ø x L) : 17 x 98 mmMax. RPM/RCF : (G)4,000 rpm / 3,032 xg (B)4,200 rpm / 3,343 xg (S)4,000 rpm / 2,700 xg (SR)4,200 rpm / 2,976 xgRadius: (G),(B)169.5mm (S),(SR)150.9mmHole bottom type : RoundMax. height for tube fit: 123(w/ cap)/127(w/o cap)

Bucket bore (ø x L) : 17 x 102.5 mmBucket Max. RPM/RCF : (G)4,000 rpm / 3,105 xg (B)4,200 rpm / 3,424 xg (S)4,000 rpm / 2,774 xg (SR)4,200 rpm / 3,059 xgRadius: (G),(B)173.6mm (S),(SR)155.1mmBottom type : ConicalMax.height for tube fit :120(w/ cap)/125(w/o cap)

Bucket GLB-bd15-100

Bucket GLB-bdc15-100

Supplied with an O-ring

Supplied with an O-ring


1. Rotors

-G- : 1236R/1248R/1248/1580/1580R

-S- : 416-SR- : 624R

-B- : 1696R


Hole dimension (ø x L) : 17 x 102.5 mmMax. RPM/RCF : (G)4,000 rpm / 3,105 xg (B)4,200 rpm / 3,424 xg (S)4,000 rpm / 2,774 xg (SR)4,200 rpm / 3,059 xgRadius: (G),(B)173.6mm (S),(SR)155.1mmHole bottom type : ConicalMax.height for tube fit : 120 mm

Bucket GLB-10-8-100

Applicable Adapters

Apdater

GAS-3(10) GAS-5(10)

None

Tube capacity 3 mL 5 mL 10 mL

Rackcapacity


1 / 41 / 6

1 / 41 / 6

1 / 41 / 6

Hole dimension(Ф x L) 13.2 x 58 mm 13.2 x 82 mm -Hole bottom type Round Open -


1. Rotors

-G- : 1236R/1248R/1248/1580/1580R

-S- : 416-SR- : 624R

-B- : 1696R



Rotor GRS-S-50-4 / GRS-SR-50-4

Hole dimension (ø x L) : 30.5 x 91 mmMax.RPM/RCF : (S)4,000 rpm / 2,700 xg (SR)4,800 rpm / 3,888 xgRadius: 150.9mmHole bottom type : FlatMax. height for tube fit: 125mm

Bucket GLB-50-50

Bucket GLB-c50-50

Applicable Adapters

Apdater

GAS-u15(50)(b100) GAS-c50(50)

None

Tube capacity 15 mL 15 mL conical 50 mL conical 50 mLRack capacity(ea/4) 1 / 4 1 / 4 1 / 4 1 / 4Hole dimension(Ф x L) 17.3 x 87 29.5 x 17.5 -

Bottom type Open Conical -

Applicable Adapters

Apdater None

Tube capacity 50 mL conicalBucket


Hole dimension (ø x L) : 29.5 x 91.5 mmBucket Max. Force : (S)4,000 rpm / 2,700 xg (SR)4,800 rpm / 3,888 xgRadius: 150.9mmHole bottom type : ConicalMax. height for tube fit: 125mm

Supplied with a ring

-S- : 416 -SR- : 624R


1. Rotors


Hole dimension (ø x L) : 17 x 93 mmBucket Max. Force : (S)4,000 rpm / 2,700 xg (SR)4,800 rpm / 3,888 xgRadius: 150.9mmHole bottom type: RoundMax. height for tube fit: 125 mm

Hole dimension(ø x L) : 17 x 93 mmBucket Max. Force : (S)4,000 rpm / 2,700 xg (SR)4,800 rpm / 3,888 xgRadius: 150.9mmHole bottom type: ConicalMax. height for tube fit: 120 mm

Bucket GLB-15-50

Bucket GLB-c15-50

Applicable Adapters

Apdater None



Applicable Adapters

Apdater None




1. Rotors

Rotor GRS-S-50-4 / GRS-SR-50-4 -S- : 416 -SR- : 624R


Rotor GRS-S-50-4 / GRS-SR-50-4

Hole dimension (ø x L) : 17 x 91.5 mmBucket Max. Force : (S)4,000 rpm / 2,700 xg (SR)4,800 rpm / 3,888 xgRadius: 150.9mmHole bottom type : RoundMax. height for tube fit: 120mm

Bucket bore (ø x L) : 17 x 91.5 mmBucket Max. Force : (S)4,000 rpm / 2,700 xg (SR)4,800 rpm / 3,888 xgRadius: 150.9mmHole bottom type : ConicalMax. height for tube fit: 120mm

Bucket GLB-d15-50

Bucket GLB-dc15-50

Applicable Adapters

Apdater None



Applicable Adapters

Apdater None




1. Rotors

-S- : 416 -SR- : 624R


Rotor GRS-S-15-6 / GRS-S-15-6A / GRS-SR-15-6

Bucket bore (ø x L) : 17 x 93.8 mmBucket Max. Force : (S)4,000 rpm / 2,826 xg (SR)4,400 rpm / 3,420 xgRadius: 158mmBucket Material : Aluminium

Bucket GLB-15-15

Applicable Adapters

Apdater None

Tube capacity 10 mL 15 mLBucket



1. Rotors

-S- : 416 -SR- : 624R


1. Rotors

1.12 Tube Recommendation for Swing-out Rotors

Adapter Hole dimension (Ø x L, mm) Tube Capacity Tube Tube Material Max. g-force(xg) Tube dimension

O.D. x h, mm

GAM-5-24(750)

3mL - 13.2 x 58 3~4mLblood tube


AMPULAB 13 x 75 mm

5mL - 13.2 x 85

4~7mL blood tube


AMPULAB 13 x 100 mm

5 mL conicalBiosigma(Snap Cap) PP 21,000 xg 17 x 60mm


GAM-10-21(750) 16 x 85 9~10 mLblood tube


AMPULAB 16 x 100 mm

GAM-15-19(750) 17.2 x 85 15mLNalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0

GAM-c15-14(750) 17x 8515 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120



Eppendorf(Snap Cap) PP 25,000 xg 17 x 60mmGAM-50-7(750) 29.2 x 87 50 mL Nalgene 3119-0050 ② PPCO 100,605 xg 28.8 x 105.9GAM-50-7(750) 29.2 x 87 50 mL Nalgene 3117-0500 ① PC 50,000xg 28.8 x 103.1

GAM-c50-5(750) 29.2 x 88 50 mL conical

Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115


GAS-250(750) 62.3 x 87 250 mL

Nalgene 3120-0250 ③ PPCO 13,200 xg 61.8 x 125.2

Nalgene 3122-0250 ③ PC 27,500 xg 61.8 x 125.2

Autofill 1801-RLS ④ PP 8,250 59 x 129

Autofill 1803-RLS ⑤ PP 27,500 59 x 129

Autofill 1802-RLS ④ PC 8,250 59 x 129

Autofill 1804-RLS ⑤ PC 31,700 59 x 129

For GRS-B-1000-4




1. Rotors



O.D. x h, mm

GAS-500(750) 75.5 x 98.7 500 mL

Nalgene 3120-9500 PPCO 13,700 xg 69.5 x 158.9

Nalgene 3122-0500 PC 13,700 xg 69.5 x 158.9

Autofill 1805-RLS ① PP 8,250 xg 68 x 196

Autofill 1807-RLS ② PP 13,500 xg 68 x 196

Autofill 1806-RLS ① PC 8,250 xg 68 x 196

Autofill 1808-RLS ② PC 13,850 xg 68 x 196

GAS-c500(750) 95.6 x 57 500 mL conical Corning 431123 PP 6,000 xg -

- 99 x 105 750 mL Thermo 75003699 PP - 98 x 133

- 99 x 105 1,000 mLNalgene 3120-1000 ③ PPCO 7,100 xg 98.0 x 174.2

Nalgene 3122-1000 ③ PC 7,100 xg 98.2 x 177.0

For GRS-B-1000-4


PP - PolypropylenePC - PolycarbonatePPCO - Polypropylene Copolymer

① Bottle with screw cap for low speeds. ② Bottle with leak-proof sealing cap for high speeds.③ To assure leakproof, especially at higher speeds and fill volumes, use sealing closure DS3132-0063.



1. Rotors



O.D. x h, mm

GAM-5-48(h1000)



AMPULAB 13 x 75 mm

5mL - 13.2 x 85

4~7mL blood tube


AMPULAB 13 x 100 mm



GAM-10-38(h1000) 16 x 85 9~10 mLblood tube


AMPULAB 16 x 100 mm

GAM-15-29(h1000) 17.2 x 85 15mLNalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0

GAM-c15-29(h1000) 17 x 90 15 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120


GAM-50-13(h1000) 29.2 x 87 50 mLNalgene 3119-0050 ② PPCO 100,605 xg 28.8 x 105.9Nalgene 3117-0500 ① PC 50,000xg 28.8 x 103.1

GAM-c50-13(h1000) 29.5 x 92.5 50 mL conical

Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115


GAM-250-2(h1000) 62 x 87 250 mL

Nalgene 3120-0250 ③ PPCO 13,200 xg 61.8 x 125.2Nalgene 3122-0250 ③ PC 27,500 xg 61.8 x 125.2Autofill 1801-RLS ④ PP 8,250 59 x 129 Autofill 1803-RLS ⑤ PP 27,500 59 x 129 Autofill 1802-RLS ④ PC 8,250 59 x 129 Autofill 1804-RLS ⑤ PC 31,700 59 x 129

For GRS-B-1000-4

Bucket GLB-h1000-1000





1. Rotors



O.D. x h, mm

GAM-10-35(r1000) 16 x 85 9~10 mLblood tube


AMPULAB 16 x 100 mm

GAM-15-28(r1000) 17.2 x 85 15mLNalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0

GAM-c15-22(r1000) 17 x 90 15 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120


GAM-50-11(r1000) 29.2 x 87 50 mLNalgene 3119-0050 ② PPCO 100,605 xg 28.8 x 105.9Nalgene 3117-0500 ① PC 50,000xg 28.8 x 103.1

GAM-c50-10(r1000) 29.5 x 92.5 50 mL conical

Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115


For GRS-B-1000-4

Bucket GLB-r1000-1000





Adapter Bore (Ø x L, mm) Tube Capacity Tube Tube Material Max. g-force(xg) Tube dimension

O.D. x h, mm

GAM-5-24(750)



AMPULAB 13 x 75 mm

5mL - 13.2 x 85

4~7mL blood tube


AMPULAB 13 x 100 mm



GAM-10-21(750) 16 x 85 9~10 mLblood tube


AMPULAB 16 x 100 mm

GAM-15-19(750) 17.2 x 85 15mLNalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0

GAM-c15-14(750) 17x 8515 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120




GAM-50-7(750) 29.2 x 87 50 mLNalgene 3119-0050 ② PPCO 100,605 xg 28.8 x 105.9Nalgene 3117-0500 ① PC 50,000xg 28.8 x 103.1

GAM-c50-5(750) 29.2 x 88 50 mL conical

Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115


GAS-250(750) 62.3 x 87 250 mL

Nalgene 3120-0250 ③ PPCO 13,200 xg 61.8 x 125.2Nalgene 3122-0250 ③ PC 27,500 xg 61.8 x 125.2Autofill 1801-RLS ④ PP 8,250 59 x 129

Autofill 1803-RLS ⑤ PP 27,500 59 x 129

Autofill 1802-RLS ④ PC 8,250 59 x 129

Autofill 1804-RLS ⑤ PC 31,700 59 x 129

GAS-c250(750) 60.5 x 125 250 mL conical Corning 430776 PP 6,000 xg 59.7 x 171.2

GAS-c500(750) 95.6 x 57 500 mL conical Corning 431123 PP 6,000 xg -

- 99 x 105 750 mL Thermo 75003699 PP - 98 x 133

For GRS-G-750-4/GRS-B-750-4

Bucket GLB-750-750 / GLB-b750-750/ GLB-b750-750B


1. Rotors




O.D. x h, mm

- 69.2 x 135 500 mL

Nalgene 3120-9500 PPCO 13,700 xg 69.5 x 158.9 Nalgene 3122-0500 PC 13,700 xg 69.5 x 158.9Autofill 1805-RLS ① PP 8,250 xg 68 x 196





Bucket GLB-500-750 / GLB-500-750B


1. Rotors

① Bottle with screw cap for low speeds. ② Bottle with leak-proof sealing cap for high speeds.





O.D. x h, mm

GAM-5-30(r750)

3mL - 13 x 56.5 3~4mLblood tube


AMPULAB 13 x 75 mm

5mL - 13 x 85 4~7mL blood tube


AMPULAB 13 x 100 mm

GAM-15-20(r750) 17.2 x 85

15mLNalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0

9~10 mLblood tube


AMPULAB 16 x 100 mm

GAM-c15-16(r750) 17.5 x 8515 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120





GAM-c50-5(r750) 29.2 x 85 50 mL conical

Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115


GAS-250(r750) 62.5 x 87 250 mL

Nalgene 3120-0250 ③ PPCO 13,200 xg 61.8 x 125.2Nalgene 3122-0250 ③ PC 27,500 xg 61.8 x 125.2Autofill 1801-RLS ④ PP 8,250 59 x 129

Autofill 1803-RLS ⑤ PP 27,500 59 x 129

Autofill 1802-RLS ④ PC 8,250 59 x 129

Autofill 1804-RLS ⑤ PC 31,700 59 x 129

GAS-c250(750) 61x 93 250 mL conical Corning 430776 PP 6,000 xg 59.7 x 171.2

GAS-c500(750) 98 x 94 500 mL conical Corning 431123 PP 6,000 xg -

For GRS-G-r750-4/GRS-B-r750-4



1. Rotors





O.D. x h, mm

- 69.2 x 135 500 mL

Nalgene 3120-9500 PPCO 13,700 xg 69.5 x 158.9

Nalgene 3122-0500 PC 13,700 xg 69.5 x 158.9

Autofill 1805-RLS ① PP 8,250 xg 68 x 196




For GRS-G-r750-4/GRS-B-r750-4

Bucket GLB-500-r750


1. Rotors


① Bottle with screw cap for low speeds. ② Bottle with leak-proof sealing cap for high speeds.




O.D. x h, mm

- 62 x 109 250 mL

Nalgene 3120-0250 ① PPCO 13,200 xg 61.8 x 125.2Nalgene 3122-0250 ① PC 27,500 xg 61.8 x 125.2Autofill 1801-RLS ② PP 8,250 59 x 129 Autofill 1803-RLS ③ PP 27,500 59 x 129 Autofill 1802-RLS ② PC 8,250 59 x 129 Autofill 1804-RLS ③ PC 31,700 59 x 129


O.D. x h, mm- 60.5 x 130.2 250 mL conical Corning 430776 PP 6,000 xg 59.7 x 171.2


Bucket GLB-250-250

Bucket GLB-c250-250


1. Rotors

① To assure leakproof, especially at higher speeds and fill volumes, use sealing closure DS3131-0038.② Bottle with screw cap for low speeds. ③ Bottle with leak-proof sealing cap for high speeds.




For GRS-G-r250-4/GRS-L-r250-4/GRS-B-r250-4



O.D. x h, mm

GAM-5-12(r250)



AMPULAB 13 x 75 mm

5mL - 13.5 x 80 4~7mL blood tube


AMPULAB 13 x 100 mm

GAM-15-12(r250) 17.5 x 90 15 mLNalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0


Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120




Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115


GAM-85-2(r250)17 x 85.6 15 mL

Nalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0

38.5 x 86.5 85 mL Nalgene 3118-0085 PC 50,000 xg 38.2 x 105.2

GAS-250(r250) 62.5 x 87 250 mL

Nalgene 3120-0250 ③ PPCO 13,200 xg 61.8 x 125.2Nalgene 3122-0250 ③ PC 27,500 xg 61.8 x 125.2Autofill 1801-RLS ④ PP 8,250 59 x 129 Autofill 1803-RLS ⑤ PP 27,500 59 x 129 Autofill 1802-RLS ④ PC 8,250 59 x 129 Autofill 1804-RLS ⑤ PC 31,700 59 x 129


1. Rotors





For GRS-G-100-6/GRS-B-100-6/GRS-S-100-4/GRS-SR-100-4Bucket GLB-b100-100


O.D. x h, mm

GAM-7-50(b100) 13.5 x 60

3~4mLblood tube


AMPULAB 13 x 75 mm

4~7 mLblood tube


AMPULAB 13 x 100 mm

GAM-10-5(b100) 16 x 60

9~10 mLblood tube


AMPULAB 16 x 100 mm

12 mLNalgene 3110-0120 ① PPCO 50,000 xg 15.8 x 102.1 Nalgene 3117-0120 ① PC 50,000 xg 15.9 x 103.1


GAM-15-3(100) 17.5 x 105 15 mLNalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0

GAS-c15(b100) 17.2 x 106.5 15 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120


GAS-50(b100) 29.5 x 95.9 50 mLNalgene 3119-0050 ② PPCO 100,605 xg 28.8 x 105.9Nalgene 3117-0500 ① PC 50,000xg 28.8 x 103.1

GAS-c50(b100) 30 x 100 50 mL conical

Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115


GAS-85(b100) 38.5 x 96.3 85 mL Nalgene 3118-0085 PC 50,000 xg 38.2 x 105.2GAS-100(b100) 44.2 x 93 100 mL Hettich 0526 Glass N/A 44 x 100


1. Rotors






O.D. x h, mm

GAS-u15(50) 17.3 x 87

15 mLNalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0

15 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120


GAS-c50(50) 29.5 x 17.5 50 mL conical

Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115


- 30.5 x 97 50 mLNalgene 3119-0050 ② PPCO 100,605 xg 28.8 x 105.9Nalgene 3117-0500 ① PC 50,000xg 28.8 x 103.1

For GRS-G-100-6/GRS-B-100-6/GRS-S-100-4/GRS-SR-100-4

Bucket GLB-b50-100Adapter Bore (Ø x L, mm) Tube Capacity Tube Tube Material Max. g-force(xg) Tube dimension

O.D. x h, mm

GAM-7-50(b100) 13.5 x 60

3~4mLblood tube


AMPULAB 13 x 75 mm

4~7 mLblood tube


AMPULAB 13 x 100 mm

GAM-10-5(b100) 16 x 60

9~10 mLblood tube


AMPULAB 16 x 100 mm

12 mLNalgene 3110-0120 ① PPCO 50,000 xg 15.8 x 102.1 Nalgene 3117-0120 ① PC 50,000 xg 15.9 x 103.1


GAM-15-3(100) 17.5 x 105 15 mLNalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0

GAS-c15(b100) 17.2 x 106.5 15 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120


GAS-50(b100) 29.5 x 95.9 50 mLNalgene 3119-0050 ② PPCO 100,605 xg 28.8 x 105.9Nalgene 3117-0500 ① PC 50,000xg 28.8 x 103.1

GAS-c50(b100) 30 x 100 50 mL conical

Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115


GAS-85(b100) 38.5 x 96.3 85 mL Nalgene 3118-0085 PC 50,000 xg 38.2 x 105.2GAS-100(b100) 44.2 x 93 100 mL Hettich 0526 Glass N/A 44 x 100


1. Rotors


① Use with friction fit closures (Cat. No. DS3111-series) for high speed centrifugation. ② For leakproof performance >10,000 xg, use a sealing closure DS3131-0024.




O.D. x h, mm

- 30 x 89 50 mL conical

Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115



O.D. x h, mm

- 17 x 102.5 15 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120



O.D. x h, mm

- 16 x 89 9~10 mLblood tube


AMPULAB 16 x 100 mm


O.D. x h, mm

- 17 x 98 15 mLNalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0

For GRS-G-100-6/GRS-B-100-6/GRS-S-100-4/GRS-SR-100-4

Bucket GLB-dc50-100

Bucket GLB-bdc15-100

Bucket GLB-10-8-100

Bucket GLB-bd15-100


1. Rotors






O.D. x h, mm

- 29.5 x 91.5 50 mL conical

Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115



O.D. x h, mm

- 17 x 102.5 15 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120



O.D. x h, mm

- 17 x 93 15 mLNalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0

For GRS-S-50-4/GRS-SR-50-4

Bucket GLB-c50-50

Bucket GLB-50-50

Bucket GLB-c15-50

Bucket GLB-15-50


O.D. x h, mm

GAS-u15(50) 17.3 x 87


15 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120


GAS-c50(50) 29.5 x 17.5 50 mL conical

Orange 4440100N

PP

12,000 xg 30 x 114Falcon 352070 16,000 xg 30 x 115


- 30.5 x 97 50 mLNalgene 3119-0050 ② PPCO 100,605 xg 28.8 x 105.9Nalgene 3117-0500 ① PC 50,000xg 28.8 x 103.1


1. Rotors






O.D. x h, mm

- 17 x 91.5 15 mL conical

Corning 430052

PP

12,000 xg 17 x 118.5Falcon 352096 12,000 xg 17 x 120



O.D. x h, mm

- 17 x 91.5 15 mLNalgene 3110-0150 ① PPCO 50,000 xg 15.8 x 112.8Nalgene 3117-0150 ① PC 50,000 xg 16.0 x 114.0


O.D. x h, mm

- 17 x 91.5



9~10 mLblood tube


AMPULAB 16 x 100 mm

Bucket GLB-dc15-50

Bucket GLB-d15-50

Bucket GLB-15-15




1. Rotors







2. Preparing and Using Fixed-angle Rotor

2.1 Preparing the Rotor

2.2 Using Tubes

1. Check the rotor,tube, Adapter for any cracks, foreign objects and pitting. [Refer to 6. Maintenance].2. Be sure the motor shaft is not damaged3. Check the level using a leveler tool after installing the rotor.4. Check the tubes you want to use are listed on the [1.8 & 1.12 Tube Recommendation].5. Refer to [Appendix 3.Chemical resistance of Plastics] before loading sample.

- Load tubes symmetrically with identical volume.- Always use the same type of tubes. (weight, material,density and volume.)- Insert sets of two tubes in opposite holes.- Opposing tubes must be filled to the same volume with sample of the same density.

[Correct arrangement]


2. Preparing and Using Fixed-angle Rotor

2.3 Installing the Rotor

1. Check the motor shaft and chamber are free of foreign objects.2. Gently insert the rotor onto the motor shaft.3. Place the rotor lid on the rotor body and turn the rotor handle clockwise until it secured.4. Make sure the device and the rotor is balanced using a leveler tool.4. For operation, refer to the operating manual of the device.

NEVER use the centrifuge unless the rotor is properly intalled.

2.4 Removing the Rotor/Sample

1. Turn the rotor handle counterclockwise and remove the rotor lid.2. Carefully remove the tubes from the rotor.3. Carefully remove the rotor from the chamber.


3. Preparing and Using Swing-out Rotor

3.1 Preparing the Rotor

3.2 Installing the Rotor

1. Check the motor shaft and chamber are free of foreign objects.2. Gently insert the rotor onto the motor shaft.3. Insert the rotor bolt into the center hole of the rotor.4. Turn the rotor bolt clockwise using the wrench.5. Make sure the device is balanced using the leveler tool.6. For Operation, refer to the operating manual of the device.

1. Check the rotor,tube, Adapter for any cracks, foreign objects and pitting. [Refer to 6. Maintenance].2. Be sure the motor shaft is not damaged.3. Check the level using a leveler tool after installing the rotor.4. Check the tubes you want to use are listed on the [1.8 & 1.10 Recommended Tubes].5. Refer to [Appendix 3&4] before loading sample.


3. Preparing and Using Swing-out Rotor

3.4 Removing Rotor/Tubes

1. Take buckets out.2. Remove Adapters and tubes from the bucket.3. Turn the rotor bolt counterclockwise.4. Remove the rotor vertically from the chamber gently.

3.3 Loading the Bucket/Adapter/Sample

1. Load the buckets on all pivots. ▶ For unused pivots, load the empty buckets. ▶ Buckets must be loaded symmetrically.

2. Swing the buckets by hand to ensure it moves smoothly.

3. Always use the same type of tubes. (weight, material,density and volume.)4. Ensure symmetrical loading, insert sets of two tubes in opposite holes.

[Correct tube arrangement for Adapters and microplates ]

[Correct bucket arrangement]

Bucket with tubes

Empty bucket


4. Bucket and Adapter

4.1 Adapter for Fixed-angle Rotor

Use only Adapters that provided by GYROZEN.

Many rotors can accommodate various tube sizes by using adapters.



4.2 Using Adapter for Fixed-angle Rotor

Make sure that the Adapter you are using is for a round tube or a conical tube to prevent damage of the device or tube.

Before use

After use

Using Adapter

Adapter storage

1. Check the Adapter for any cracks or foreign objects.2. Check the Adapter hole is free of foreign objects or moisture.

1. Remove the Adapter from the rotor. 2. Check for Adapter for sample leaks. If necessary, clean or autoclave it.

1. Install the rotor onto the motor shaft.2. Load adapters into the rotor.3. Load tubes into the Adapters.

1. Keep the Adapter in a dry place. 2. Keep the Adapter out of the direct rays or heater.

There is a hole on the bottom of the Adapter.

The bottom of the Adapter is blocked.(No hole)

Adapters for Conical tubes Adapters for Round tubes


4.3 Bucket

Swing-out rotor holds tubes using the buckets and adapters.The bucket swing out to a horizontal position while centrifugation.


Use only buckets that provided by GYROZEN.


Before Use

After Use

Using Bucket

Adapter Strorage

1. Check the bucket for any cracks or foreign objects. Particularly frequent use makes it easy for foreign objects to stick on the bucket grooves.2. Check the bucket grooves are lubricated. 3. Swing the bucket with a hand to check for the bucket moves smoothly.

1. Remove the bucket from the rotor.2. After run, check the Adapter for sample leaks. If necessary, clean or autoclave the bucket.

1. For buckets with lid, remove the lid.2. Keep the bucket upside down on a flat place.3. Always keep the bucket empty.

1. Install the rotor onto the motor shaft.2. Hang the buckets on the rotor pivot.3. Load the Adapters and tubes.

4.4 Using Bucket


Many buckets can accommodate various tube sizes by using adapters.


Use only Adapters that provided by GYROZEN.

4.5 Adaptor for Swing-out Rotor


Swing-out rotors can accommodate various tube sizes by using adaptors.


4.6 Using Adapters for Swing-out Rotor

Before Use

After Use

Using Adapter

Adapter Storage

1. Remove the Adapters from the bucket.2. After run, check the Adapter for sample leaks. If necessary, clean or autoclave the Adapter.

1. Install the Swing-out rotor onto the motor shaft.2. Hang the buckets on the rotor pivots.3. Load the Adapters and tubes.


1. Check the Adapters for any cracks or foreign objects.2. When using the 3-stage Adapter, assemble them to fit the bucket groove line. [Refer to 6.4 Inspection-Adapter]2. When using the 3-stage Adapter, insert the handle to the handle groove.3. Swing the bucket with a hand to check for the bucket moves smoothly.4. Make sure that the Adapter you are using is for a round tube or a conical tube to prevent damage of the device or tube.

1. Keep the Adapter in a dry place. 2. Keep the Adapter out of the direct rays or heater.


5.1 Using Tubes, Bottles

Correct arrangement for Angle rotor

Correct arrangement for Swing-out rotor Wrong arrangement for Swing-out rotor

An improperly loaded rotor will be unbalanced.

Balancing

5. Tubes, Bottles

- Load tubes symmetrically with identical volume.- Always use the same type of tubes. (weight, material,density and volume.)- Insert sets of two tubes in opposite holes.- Opposing tubes must be filled to the same volume with sample of the same density.


5.2 Filling Volume

5.3 Tube/Bottle Sterilization

- It is generally recommended to fill more than 80% of the the tube or bottle. However, there is an exception that must be filled to the end of the container to prevent damage. Thus the minimum and maximum filling volumes should be different in accordance with the manufacturer's guidelines.

- There is a risk of tube breakage when filling volume of sample is too less compared to the tube capacity. In this case, use a tube and adapter that meet the sample volume.

Observe manufacturer’s sterilization guidelines.

Wrong use of tubes

Different Caps Different DensityDifferent Volume Different Tubes

5. Tubes, Bottles


6. Maintenance

6.1 Inspection - Angle Rotor

(*1) After run, make sure that the sample is not leaked to the rotor hole. If the rotor has been contaminated by aggressive chemicals, immediately clean it by means of a mild cleaning agent.

Check the rotor as follows after or before every use.

1. Make sure that the rotor thread is free of wear .

3. Make sure that there is no cracks or other signs of damage on the rotor body.

2. Make sure that there is no foreign objects in the rotor holes.(*1)

4. Make sure that there is no foreign objects on the drive hole.

5. Make sure that there is no foreign objects on the motor shaft.

Rotors and accessories are may damaged in repeated use or careless handling. And damage may be worsened under centrifugal forces.

①

②

③

④

⑤


2. Make sure that there is no foreign objects in the rotor holes.(*1)


5. Make sure that there is no foreign objects on the motor shaft.

6.3 Inspection - Bucket

1. Make sure that there is no foreign objects in the bucket.

3. Make sure that the bucket grooves are clean. Regularly lubricate the bucket grooves.

1. Make sure that there is no foreign objects on the rotor pivots.

2. Make sure that there is no foreign objects on the bucket threads. (Only for a bucket using a cap)


4. Make sure that there is no cracks or other signs of damage on the bucket.

①

②

②

③

③

④

④

6. Maintenance

6.2 Inspection - Swing-out Rotor

2. Make sure that the threads are free of wear .

3. Make sure that there is no cracks or other signs of damage on the rotor.

①


1. Make sure that there is no foreign objects in the Adapter.

6.4 Inspection - Adapter

1. Make sure that there is no foreign objects in the adapter hole.

3. Make sure that the Adapter you are using is for a round tube or a conical tube to prevent misuse.

3.When using the disk Adapter, assemble them to fit the bucket

4. When using the 3-stage adpater, insert the handle to the handle groove.

2. Make sure that there is no cracks or other signs of damage on the Adapter.

①

①

②

②

③

③

④

6. Maintenance

2. Make sure that there is no cracks or other signs of damage on the Adapter.


6.5 Storage - Angle Rotor

6.6 Storage - Swing-out Rotor

1. After centrifugation, remove the rotor from the chamber.2. Remove the lid and keep it upside down. 3. Spread the grease lightly on the rotor thread after cleaning or periodically.

1. After using the rotor, remove the rotor from the chamber.2. Keep the rotor on a flat surface.3. Spread the grease lightly on the rotor pivot after cleaning or periodically.

6. Maintenance


6.7 Cleaning - Rotor

Clean the rotor immediately if salts or other corrosive materials are leaked.

When foreign objects adheres to the rotor

1. Wash the rotor with a mild detergent(pH 5-9), using a soft brush.2. Rinse thoroughly with distilled water. 3. Wipe off the moisture with a soft cloth and dry the rotor upside down

Soak the rotor in warm water for a while, then gently remove foreign objects with a soft brush.

[Angle Rotor]After drying the rotor, spread the grease lightly on the rotor threads.[Swing-out Rotor]After drying the rotor, spread the grease lightly on the rotor pivot.

- Do not use sharp tools on the rotor, as the surface can get scratched.- Do not use any aggressive chemicals on the rotor, such as acetone, strong acids/base, or formaldehyde etc.

6. Maintenance


Clean the rotor immediately if salts or other corrosive materials are leaked.

- Do not use sharp tools on the rotor, as the surface can get scratched.- Do not use any aggressive chemicals on the rotor, such as acetone, strong acids/base, or formaldehyde etc.

6.8 Sterilizing Rotor

1. Remove the lid and place the rotor upside down in an autoclave. : Autoclave at 121°C for 20 minutes.2. Remove any contaminants with a cloth soaked in ethanol(70%).

Check the proper time and temperature before autoclaving.Do not put the rotor in boiling water.

- Ethanol is a flammable substance.- Do not use ethanol near the centrifuge.

6. Maintenance


6.9 Cleaning - Bucket

6.10 Sterilizing Bucket

6. Maintenance

Clean the bucket immediately if salts or other corrosive materials are leaked.

1. Wash the bucket with a mild detergent(pH 5-9), using a soft brush.2. Rinse thoroughly with distilled water. 3. Wipe off the moisture with soft cloth and dry the bucket upside down

- Do not use sharp tools on the bucket, as the surface can get scratched.- Do not use any aggressive chemicals on the bucket, such as acetone, strong acids/base, or formaldehyde etc.

- Autoclave at 121°C for 20 minutes.

Observe time and temperature when using an autoclave.Do not autoclave the bucket lid.


- Do not use sharp tools on the bucket, as the surface can get scratched.- Do not use any aggressive chemicals on the bucket, such as acetone, strong acids/base, or formaldehyde etc.

6.11 Cleaning - Adapter

6.12 Sterilizing Adapter

1. Wash the Adapter with a mild detergent (pH 5-9) using a soft brush.2. Do not use sharp tools on the Adapter, as the surface can get scratched.3. Rinse thoroughly with distilled water. 4. Wipe off the moisture with soft cloth.

Clean the adapter immediately if salts or other corrosive materials are leaked.

6. Maintenance

Observe time and temperature when using an autoclave.

1. Autoclave at 121°C for 20 minutes.


7. Troubleshooting

7.1 Rotor

Problem Solution

Lid can not be tightened - Check that the rotor threads are greased.- Check the rotor threads and motor shaft for foreign matters.

Rotor is discolored - When cleaning the rotor, it may appear discolored if not rinsed thoroughly. Rinse the rotor thoroughly with water.- Anodizing may be worn. Contact GYROZEN.

Rotor ID. is not identified Check the drive hole for foreign matters.

Noise and vibration duringrunning

Please re-check the coupling status of the following three matches tominimize the noise.1. the balanced way of coupling of the rotor into the motor shaft.2. the completeness of fixing of the rotor locking nut on the rotor.3. the matching status of rotor lid with the rotor.


7. Troubleshooting

7.2 Tube

Problem Solution

Sample leaks out

- Close the tube cap tightly.- Tube volume exceeds maximum filling volume. Refer to the tube manufacturer’s guideline for tube filling volumes.

Tube is cracked

- Tubes may crack if they are used below their lower temperature limit or higher g-force. Refer to the tube manufacturer’s guideline for temperature limit and Max. g-force.- Repeated use of tube may cause cracks. Dispose of cracked tubes.

Tube is broken

- Sample volume is too low to support tube wall. Refer to the tube manufacturer’s guideline for tube volumes.- Tubes run above their rated speed. Refer to the tube manufacturer’s guideline for maximum speed of tubes.


Appendix 1.

Pelleting, Differential Centrifugation

Differential Centrifugation

Centrifugation Separations

The most common centrifugation method is pelleting.Pelleting separates particles of different sedimentation coefficients.During centrifugation, the largest particles in the sample traveling to the bottom of the tube first.Differential centrifugation is the successive pelleting of particles from previous supernatant of decreasing sedimentation velocities, using increasingly higher forces and/or long run times.Through centrifugation two fractions are gained. A pellet and a supernatant of unsedimented material.The supernatant can be re-centrifuged at higher speeds to get a new pellet and supernatant.

Centrifugation Time

Cent

rifug

al F

orce

A. B. C. D.


Appendix 1.

Nucleic Acids

Yeast tRNA

1

2

3

4

6

7

8

9

20

40

60

80

200

400

600

800

2000

4000

6000

8000

Viruses

Rous sarcomaFeline leukemiaBacteriophage T2

PoliomyelitisTobacco mosaicEquine encephalitis

E. coli rRNACalf liver DNAVesicular stomatitis virus RNABacteriophage T5 DNABacteriophage T2 & T4 DNAs

Broad bean mottle

SubcellularParticles

Soluble Proteins

Plasma membranes

Cytochrome c

Collagen

Albumin

Luteinizing hormone

Immunoglobulin G

Aldolase

Catalase

-Macroglobulin

5

0

10

100

1 000

10 000

100 000

Mitochondria

Microsomes

Polysomes

Ribosomes

Ribosomal subunits

Sedimentation Coefficients


Density gradient Centrifugation

Samples are layered on top of the density gradient. Under centrifugal force, particles sediment through the gradient column in zones. Each zone consisting of size, sedimentation rate of particles.When applying the sample to the density gradient, insert it carefully so that it does not mix with the density gradient.The centrifugation should be stopped before any of the separated zones reach the bottom of the tube.

Isopycnic gradient separates particles on the basis of density rather than size, independent of time.Each particle sediments in the tube where the gradient density is equal to the particle density.Unlike Rate-zonal centrifugation method, density gradient and the sample are mixed in the tube.

Density gradient Centrifugation permits for the complete separation of several or all components in a sample. Tube is partially filled with a solution having a decreasing density from the bottom to the top of the tube. This method is mainly suited to the viruses.There are two methods in which density gradients may be used. : Rate-zonal and isopycnic.

Rate-zonal Centrifugation

Rate-zonal Centrifugation Isopycnic Centrifugation

Isopycnic Centrifugation

Sample zone

Density gradient

Cent

rifug

al F

orce

A. B. C.

Cent

rifug

al F

orce

A. B.

Appendix 1.


Density gradient medium

Appendix 1.

Gradient medium type Principle uses

Polyhydric alchols

- Sucrose Oraganelles, membrane vesicle, virus, protein, ribosome, polysome

- Glycerol Mammalian cells(infrequent), protein

- Sorbitol NonMammalian subcellular particles

- Ficoll, polysucrose and dextrans

Mammalian cells(sometimes in combination with iodinated density gradient media), mammalian subcellular particles(infrequent)

Inorganic salts

- CsCl DNA, Virus, Protein

- Cs2SO4 DNA, RNA

- KBr Plasma lipoprotein

Iodinated gradient media

- Diatrizoate Mainly as component of commercial lymphocyte isolation media

- Nycodenz Histodenz Mammalian cells, organelles, membrane, vesicle, virus

- Percoll Mammalian cells, organelles, membrane, vesicle(infrequent)


RCF calculation

Separation time

RCF (relative centrifugal force) refers to the amount of acceleration or force exerted on the sample in a centrifuge.RCF is obtained from the below formula.

The sedimentation time t is calcurated using sedimentation codfficients s and k factor of the rotor.

rmax : Max. Rotor Radius rmin : Min. Rotor Radius K : k-factor (rotor efficancy)S : Sedimenation coefficient

Appendix 2.


Tube/Bottle Material polypropylene Polycarbonate Polypropylene

Copolymer

Acids(dilute or weak) S M S

Acids S U S

Alcohols S U S

Aldehydes M M M

Bases S U S

Esters M U M

Hydrocarbons(aliphatic) M U M

Hydrocarbons(aromatic) M U M

Hydrocarbons(halogenated) M U M

Ketones M U M

Oxidizing agents(strong) U M U

S: Satisfactory resistance M: Marginal resistance U: Unsatisfactory resistance

※ This information has been assembled from a variety of sources.Resistance may vary depending on temperature and pressure.To prevent failure, always recommend test run before use.

Appendix 3.

Chemical resistance of Plastics

Before inserting the sample into the tube, refer to below table.


Gradient MediumCesium acetate MCesium bromide MCesium chloride MCesium formate MCesium iodide MCesium sulfate MDextran sulfate MFicoll paque MGlycerol MMetrizamide MRubidium bromide M

Rubidium chloride M

Sodium bromide USucrose MSucrose, alkaline M

Gradient MediumAcetone(50%) SAmyl alcohol SBenzene SCresol SDiethyl ether SEthyl acetate MEthyl alcohol SMethylene chloride U

S: Satisfactory resistance M: Marginal resistance U: Unsatisfactory resistance

Appendix 4.

Chemical resistance of Aluminum

Gradient MediumAcetic acid S

Acetic acid(5%) SAcetic acid(60%) S

Acetic acid(glacial) U

Aqua regia UChromic

acid(10%) U

Chromic acid(50%) U

Hydrochloric acid(10%) U

Hydrochloric acid(35%) U

Hydrofluoric acid(10%) U

Hydrofluoric acid(50%) S

Lodoacetic acid SOleic acid UPicric acid U

Sulfuric acid(10%) U



Before inserting the tubes into the rotor, refer to below table.

※ This information has been assembled from a variety of sources.Resistance may vary depending on temperature and pressure.To prevent failure, always recommend test run before use.

Before inserting the tubes into the rotor, refer to below table.

Rotor & Accessories Manual

© 2019 GYROZEN

- This contents in this manual are subject to change for improvement without notice.

RECYCLABLE

GYROZEN Co., Ltd.30-12 Gyeryong-ro 141-gil, Yuseong-gu, Daejeon 34187, KoreaTel: +82-42-719-8200 Fax: +82-42-826-9848www.gyrozen.com

[email protected]ㅣ[email protected] Doc.No. : C99DC002PD1904

Documents

Operation Manual - GYROZEN · 2019. 7. 5. · 6.1 Inspection - Fixed Angle Rotor - 80 6.2 Inspection - Swing-out Rotor - 81 6.3 Inspection - Bucket - 81 6.4 Inspection - Adapter -