Only ONE Change to Dramatically Improve

ZORBAX®

SB METHODS

Agilent® Technologies

Core-Shell Technology

The simple change

is…

0

0

100

200

mAU

20860

2 4 6 8 10 min

a

b c d*

0 2 4 6 8 10 12 14 min

a

b c d

0

100

mAU

20859

*

20785

6 8 10 12 14 min

0

100

200

mAU

Kinetex 5 µm core-shell columns provide considerable improvements for methods devel-oped on standard HPLC systems with conventional 3 µm or 5 µm fully porous columns - at low backpressures.

Agilent, StableBond, and ZORBAX are registered trademarks of Agilent Technologies, Inc. (Palo Alto, CA, USA). Phenomenex is not affiliated with Agilent Technologies. Comparative separations may not be representative of all applications

…Replace your 3 or 5 µm ZORBAX® StableBond® (SB) Column with a

NEW Kinetex 5 µm Core-Shell HPLC Column

™

5 µm

Conditions for all columns:Columns: Kinetex 5 µm XB-C18

ZORBAX 5 µm SB-C18Dimensions: 150 x 4.6 mm

Mobile Phase: A: Water with 0.1% TFAB: Acetonitrile with 0.1% TFA

Gradient: Time (min)0 20

% B10 70

Flow Rate: 1.2 mL/minTemperature: Ambient

Detection: UV @ 210 nmSample: Pharmaceutical degradation sample

Better Chromatographic Performance• HigherEfficiency

• ImprovedSensitivity

• IncreasedResolution

Increased Resolution by substituting fully porous column with Kinetex 5 µm

Higher Productivity by then reducing column length

Lower Costs by lastly adjusting gradient profile proportionally

20784

6 8 10 12 14 min

0

100

200

mAU

Phenomenex Kinetex® 5 µm XB-C18

Agilent® Technologies ZORBAX® 5 µm SB-C18

120 Bar

Rs = 1.6

120 Bar

Rs = 1.3

22 % increase in resolution

Ap

p ID

207

84A

pp

ID 2

0785

a

b c d0

20

40

60

80

100

120

140

160mAU

20766

0 10 20 min

*0

20

40

60

80

100

120

140

160mAU

20767

0 10 20 min

a

b c d*

Conditons for all columns, except where notedColumn: Kinetex 5 µm C18

Fully porous 5 µm C18Dimensions: 250 x 4.6 mm

Mobile Phase: A: TFA/Water/THF (5/900/100) B: TFA/Acetonitrile/THF (5/900/100)

Gradient: Time (min) % B 0 10 40 80

Flow Rate: 1.5 mL/minTemperature: 40 °C

Detection: UV @ 295 nmSample: 1. Impurity a 4. Unidentified (*)

2. Impurity b 5. Paroxetine3. Impurity c 6. Impurity d

Ap

p ID

207

66

Ap

p ID

207

67

Kinetex® 5 µm XB-C18

Kinetex® 5 µm XB-C18

Fully Porous 5 µm C18

Kinetex® 5 µm XB-C18

Dimensions: 150 x 4.6 mm Dimensions: 150 x 4.6 mmGradient: Time (min) % B

0 10 24 80

Critical Advantages of Increased Resolution: •Savetimeandmoney byadjustingmethod

•Separatecriticalpairsordifficultmixtures

•Lessriskoffailingsystem suitabilityrequirements

Ap

p ID

208

59

Ap

p ID

208

60

•Shorterruntime

•Morerunsperday•Lesssolventused•Reducedisposal

costs

Drop-in a Kinetex 5 µm Columnand instantly attain . . .

Drop-in Kinetex 5 µm + Simple Method Adjustmentsto achieve...

OR

See Kinetex 5 µm on Your Method Inlessthan2minutes,theKinetex5µmCalculator computes the performance and cost benefits achievablebyreplacingyourfullyporouscolumn.

http://www.phenomenex.com/methodmakeover

Phenomenex l WEB: www.phenomenex.com Phenomenex l WEB: www.phenomenex.com

Only ONE Change to Dramatically Improve

ZORBAX®

SB METHODS

Agilent® Technologies

If you are not completely satisfied withKinetex core-shell columns, send in yourcomparative data to a similar productwithin45daysforaFULLREFUND.

Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.

Trademarks PhenomenexandKinetexareregisteredtrademarks,SecurityGuardisatrademarkofPhenomenex.Agilent, StableBond, and ZORBAX are registered trademarks of Agilent Technologies, Inc.

Disclaimer Phenomenex is not affiliated with Agilent Technologies. Comparative separations may not be representative of all applications.

© 2012 Phenomenex, Inc. All rights reserved.FL96

4908

12_W

Kinetex® 5 µm Columns (mm)

SecurityGuard™ ULTRA Cartridges*50 x 2.1 3/pk

XB-C18 00B-4605-AN AJ0-8782C18 00B-4601-AN AJ0-8782PFP 00B-4602-AN AJ0-8787Phenyl-Hexyl 00B-4603-AN AJ0-8788

for 2.1 mm ID

SecurityGuard ULTRA Cartridges*50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 3/pk

XB-C18 00B-4605-E0 00D-4605-E0 00F-4605-E0 00G-4605-E0 AJ0-8768C18 00B-4601-E0 00D-4601-E0 00F-4601-E0 00G-4601-E0 AJ0-8768PFP 00B-4602-E0 00D-4602-E0 00F-4602-E0 00G-4602-E0 AJ0-8773Phenyl-Hexyl 00B-4603-E0 00D-4603-E0 00F-4603-E0 00G-4603-E0 AJ0-8774* SecurityGuard ULTRA cartridges require holder, Part No. AJ0-9000. Kinetex 5 µm columns are also available in 21.2 mm ID.

for 4.6 mm ID