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JOURNAL OF CLINICAL MICROBIOLOGYVOLUME 3 NUMBER 6 JUNE 1976
EDITORIAL BOARDALBERT BALOWS, Editor-in-Chief (1979)
Center for Disease Control,Atlanta, Ga.
LORRAINE FRIEDMAN, Editor (1979)Tulane University Medical School,
New Orleans, La.
HENRY D. ISENBERG, Editor (1979)Long Island Jewish-Hillside Medical Center,
New Hyde Park, N. Y.NATHALIE J. SCHMIDT, Editor (1980)
California Department of Health,Berkeley, Calif
Daniel Amsterdam (1977)Libero Ajello (1976)William B. Cherry (1978)Walter R. Dowdle (1976)V. R. Dowell, Jr. (1977)Richard W. Emmons (1977)John C. Feeley (1977)James D. Fenters (1976)Sydney M. Finegold (1976)Maxwell Finland (1977)Herman Friedman (1978)Thomas L. Gavan (1977)Morris A. Gordon (1978)Dieter Groschel (1977)Leonor D. Haley (1977)James L. Hardy (1978)Melvin T. Hatch (1978)Harry R. Hill (1977)
Lillian V. Holdeman (1976)Milton Huppert (1978)S. S. Kalter (1978)Leo Kaufman (1977)George E. Kenny (1976)George P. Kubica (1976)Howard W. Larsh (1977)James D. MacLowry (1977)Harold Markowitz (1977)William J. Martin (1977)John M. Matsen (1977)Joseph L. Melnick (1976)Willum I. Metzger (1977)Maurice A. Mufson (1977)Barbara G. Painter (1977)Demosthenes Pappagianis (1977)Robert H. Purcell (1977)William E. Rawls (1977)
Glenn D. Roberts (1977)Jon E. Rosenblatt (1977)Margarita Silva-Hutner (1977)James W. Smith (1977)Louis DS. Smith (1978)Peter B. Smith (1976)Alex C. Sonnenwirth (1977)Welton I. Taylor (1977)John H. Thompson, Jr. (1977)Richard C. Tilton (1978)Marvin Turck (1977)Alexander von Graevenitz (1977)Kenneth W. Walls (1977)Lewis W. Wannamaker (1978)Robert E. Weaver (1978)Irene Weitzman (1978)Robert P. Williams (1978)Marion E. Wilson (1977)
Robert A. Day, Managing EditorGisella Poliock, Director of Editorial Services
Leslie Frymire, Production Editor1913 I St., N. W., Washington, D.C. 20006
EX OFF]Helen R. Whiteley, President (1975-1976)
J. Mehsen Joseph, Secretary
The Journal of Clinical Microbiology, a publication of theAmerican Society for Microbiology, 1913 I St., N. W., Wash-ington, D.C. 20006, is devoted to the dissemination of newknowledge concerning the applied microbiological aspects ofhuman and animal infections and infestations, particularlyregarding their etiologic agents, diagnosis, and epidemiology.Papers dealing with antibiotics and antimicrobial agents andchemotherapy, with fundamental aspects of infection andimmunity, and with food or dairy microbiology fall within thescope of other ASM publications. The Journal is publishedmonthly, and the twelve numbers are divided into two volumesper year. The nonmember subscription price is $50 per year.The member subscription price is $13 per year. Single copiesare $5. Correspondence relating to subscription, reprints,
Harlyn 0. Halvorson, Vice-President (1975-1976)Brinton M. Miller, Treasurer
defective copies, availability of back issues, lost or late proofs,disposition of submitted manuscripts, and general editorialmatters should be directed to the ASM Publications Office,1913 I St., N.W., Washington, D.C. 20006 (area 202833-9680).
Second-class postage paid at Washington, D.C. 20006, and atadditional mailing offices.Made in the United States of America.Copyright © 1976, American Society for Microbiology.1976, American Society for Microbiology.All Rights Reserved.
Author IndexBlattner, W. A., 593Blazevic, Donna J., 556Brashear, Mary D., 640Brock, Susan, 582
Chamberland, Mary, 582Clark, W. A., 632Clinard, Elva H., 604
Dart, R. K., 635
Evans, Charles A., 576
Fedorko, J., 599Fossieck, B. E., Jr., 599Fraumeni, J. F., Jr., 593
Gabridge, Michael G., 560Giard, D. J., 593Gunnell, M., 593
Hall, M. Marsha, 643Hebert, G. A., 609
Hines, Paula, 640
Ilstrup, Duane M., 643Ishizaki, Hiroshi, 545Iwatsu, Tokio, 545
Kalmakoff, J., 637Kaplan, N. M., 593Kariya, Hideo, 545
Lambe, Dwight W., Jr., 586Lubiniecki, A. S., 593Lunceford, Carl D., 613Lund, Maryls E., 556Lyons, Robert W., 582
Mason, T., 593Moroz, David A., 586Myers, Donald M., 548
Nakamura, Yoichi, 545
Otto, L. A., 566
Parker, R. H., 599Parkinson, A. J., 637Pickett, M. J., 566Prince, Alfred M., 626
Rubin, Sally J., 582
Saunders, George C., 604Shepard, Maurice C., 613Smith, Rodney F., 640Stevens, Robert J., 576Stretton, R. J., 635
Triantafellu, N., 593Tzianabos, T., 609
Vnek, John, 626
Washington, J. A., II, 643Wheat, Robert, 545White, L. A., 609Williams, D. N., 556Wong, James, 640
JOURNAL OF CLINICAL MICROBIOLOGY
INSTRUCTIONS TO AUTHORS
Correspondence. Submit manu-scripts in duplicate (original andone copy) to ASM PublicationsOffice, 1913 I St., N. W., Wash-ington, D.C. 20006.
General policy. Any manuscriptsubmitted must be a report of un-published original research or clin-ical investigation, which is notbeing considered for publicationelsewhere. Each manuscriptshould present the results of anindependent, cohesive study;numbered series papers are dis-couraged.
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Nomenclature of microorga-nisms. In general, the nomencla-ture for bacteria presented inBergey's Manual of DeterminativeBacteriology (8th ed., 1974) isused. If an author challenges thisnomenclature, his own judgmentwill be followed, but the name inBergey's Manual should follow inparentheses the first time thename is used in the text and inthe Abstract. Papers which in-clude extensive taxonomic mate-rial (e.g., description of new taxa)will not be published in this jour-nal. The proper place for publica-tion of taxonomic material is theInternational Journal of System-atic Bacteriology (IJSB), which ispublished by the ASM for the In-ternational Association of Micro-biological Societies. If the mainthrust of such a paper is not tax-onomy, the paper should be di-vided, the taxonomy portion be-ing submitted to IJSB and theother portion to this journal.
Notes. The accepted form forNotes is somewhat different fromthe foregoing. Contributors shouldconsult a recent issue of the Jour-nal for style. Notes should not ex-ceed 500 words. The abstractshould not exceed 25 words.
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INDEX TO ADVERTISERSPage
*Analytab Products, Inc...................................... Cover 2*Becton Dickinson ...................................... 12, 13*Bioquest..... 9, 17
Capco, Clinical Analysis Products Co...................................... 11*Clinical Sciences, Inc...................................... Cover 4*Difco Laboratories ...................................... 20Gibco Diagnostics ...................................... 7*Lab-Tek Products Co...................................... Cover 3*Microbiological Associates ............ .......................... 10Precision Dynamics Corporation ....................................... 14*Roche Diagnostics ...................................... 18, 19*Scott Laboratories, Microbiological Sciences, Inc............................... 15Wedco, Inc. ........................................ 8
* Sustaining Member, American Society for Microbiology.
Advertising in this journal is limited to products and services believed to be of interest to the readers. However, ASMdoes not test nor examine advertised products nor claims related thereto. Therefore, ASM endorsement or approval ofadvertised products should not be inferred.
An invitation to attend
ASM Conference onCLINICAL LABORATORY IMMUNOLOGY
Friday and Saturday, October 15 and 16, 1976Sheraton-Park Hotel, Washington, D.C.
This Conference will focus attention on newer developments in laboratory immu-nology which are directly relevant to clinical patient care as well as to a wide varietyof biomedical disciplines, including antimicrobial immunity, autoimmunity, trans-plantation immunity, and cancer immunology.
Session 1. Humoral and Cellular Components of the Immune SystemSession 11. Immunodiagnosis in Infectious DiseasesSession III. Laboratory Examinations for Allergy, Immunodeficiency, and Auto-
immune DiseasesSession IV. Transplantation and Tumor Immunology
For further information, contact:Executive SecretaryAmerican Society for Microbiology1913 (Eye) Street, N.W., Washington, D.C. 20006
AUTHOR INDEX
VOLUME 3
Achanzar, David, 206Aikawa, T., 206Akiya, F. I., 21Alexander, David N., 42Alexander, Jane J., 381Allen, Alfred M., 465Alley, Cynthia C., 180Alon, Shlomit, 227Altmann, G., 390Amdur, B., 474Anderson, Richard A., 382
Bae, H. C., 211Bagley, L. R., 513Bahnemann, Hans G., 209Baker, C. N., 425Barden, Gertrude, 246Barile, Michael F., 110Barnard, Tom, 382Baron, Ellen S., 330Baron, Samuel, 223Bartenstein, Lynne, 8Bartlett, John G., 133Bartlett, Raymond C., 327Ben-Moshe, Hana, 227Bernstein, R. S., 474Bessudo, David M., 486Bettini, T. Manlio, 203Beutner, Karl R., 469Blakemore, William S., 264Blattner, W. A., 593Blazevic, Donna J., 313, 556Blue, J. L., 149Bodey, Gerald P., 14Boncyk, L. H., 463Bradbury, Janet M., 449Brady, Margaret I., 524Brashear, Mary D., 640Brasier, Francoise, 309Brock, Susan, 582Brooks, John B., 34, 180, 239Brosi, B. J. M., 541Brown, J., 149Bruun, Johan N., 264Buckingham, Judy M., 443Burgdorfer, W., 51Burson, G. Gene, 251, 258
Cahan, D., 390Cannon, Hubert, 465Cannonico, P. G., 513Carmody, Patricia, 469Carter, G. R., 344Casper, E. A., 51Chalgren, Sarah L., 359Chamberland, Mary, 582Chambers, R. W., 364Chester, Brent, 119
Chow, Anthony W., 128Chu, Fred C., 402Clark, W. A., 632Clinard, Elva H., 604Cole, Francis E., Jr., 460Cole, John R., Jr., 537Coleman, R. Marie, 281Corbett, Dorothy S., 306Coriell, Lewis L., 264Couch, Robert B., 309Cox, James H., 96Cummins, A. C., 5Cunningham, Patrick J., 128
D'Arcangelis, Dora C., 338Dajani, Adnan S., 318Dart, R. K., 635David, Hugo L., 414Davis, R. B., 149Deibel, Rudolf, 397DeWitt, Wallis E., 486DeYoung, Donald R., 47Diaz, Ramon, 203DiSalvo, Arthur F., 306Dizon, Delia, 211Dobos, P., 373Dorn, Gordon L., 251, 258Dowdle, Walter R., 233Dowell, V. R., Jr., 291Downie, Jean C., 233Drake, C. R., Jr., 359Duncan, W. Christopher, 14
Edberg, Stephen C., 49Eddy, Gerald A., 460Ederer, Grace M., 42Edwards, Earl A., 339Edwards, L. R., 501Elin, R. J., 21Evans, Charles A., 576Evans, G. L., 496
Facklam, R. R., 501Falco, Eleanora B., 453Farmer, J. J., III, 206, 350Fedorko, J., 599Feeley, John C., 486Finkelstein, Richard A., 382Flower, Margaret, 246Fossieck, B. E., Jr., 599Fraumeni, J. F., Jr., 593Freeburg, Pat W., 443Fuchs, Peter C., 75Furminger, I. G. S., 524
Gaafar, Hassan A., 438Gabridge, Michael G., 560Galphin, Judith C., 233
Gangarosa, Eugene J., 143Gelbart, Sheldon M., 62Gerichter, Ch. B., 390Gerna, G., 364Gershman, M., 214Giard, D. J., 593Gilman, Robert H., 456Gorbach, Sherwood L., 133Gordon, L. P., 102Goss, W. A., 406Grabowski, Marion W., 110Graves, S. R., 72Gray, Samuel B., Jr., 1Grove, S. B., 474Green, James H., 1Greenberg, J., 324Greenberg, Stephen B., 309Greenfield, Brian, 327Greenlee, Herbert B., 62Grillner, Lena, 86Gross, Peter A., 246Gunnell, M., 593Guze, Lucien B., 128
Hall, M. Marsha, 212, 643Harrell, W. Knox, 1Hawkins, Jean E., 453Haynes, John R., 251, 258Hebert, G. A., 609Hennessy, Joan N., 385Hentges, David J., 218Hickman, F. W., 350Higbee, James W., 137Hilderbrand, Richard L., 339Hines, Paula, 640Hinton, Norman A., 385Ho, Monto, 272Hochstein, H. D., 21Holdeman, Lillian V., 432Holder, Ian A., 175Hollick, Gary E., 302Hollis, D. G., 425Hornick, Richard B., 456Huppert, M., 186
Iannelli, Domenico, 203Iida, H., 206Ilstrup, Duane M., 212, 643Irwin, Gilbert R., 465Ishizaki, Hiroshi, 545Ito, H6mu, 91Iwatsu, Tokio, 545
Jacobs, Nicholas J., 287Jenkin, H. M., 72Jerris, Robert C., 506Jimenez-Ulate, F., 359Johns, Ronald W., 137
ii AUTHOR INDEX
Johnson, Dwight R., 533Johnson, Eugene A., 105Johnson, James E., 157, 334Johnson, Joyce B., 402Johnson, J. L., 359Jones, Dan, 309Jones, James R., 382Jones, Ronald N., 75Jones, W. D., Jr., 324Jones, Wallis L., 480Jordan, F. T. W., 449
Kalmakoff, J., 637Kalter, S. S., 463Kaplan, M. M., 593Kaplan, William, 272Kariya, Hideo, 545Karlson, Alfred G., 47Katz, David, 227Kaufman, Leo, 191Kaul, Bansi L., 469Kaye, Donald, 67Keen, Barbara M., 318Kellogg, Douglas S., Jr., 34Kennett, Margery L., 528Kenyon, R. H., 513Klein, George C., 458Knight, Ralph A., 67Knight, S. T., 72Knight, Vernon, 309
Lambe, D. W., Jr., 281, 506, 586Larghi, 0. P., 26Larsson, Lennart, 81Laslie, W. Wayne, 281Laver, W. Graeme, 233Lewis, F. A., 528Lewis, Jay F., 381Littlejohn, Dewitt C., 402Lombard, G. L., 291Lubiniecki, A. S., 593Lunceford, Carl D., 613Louie, Thomas J., 133Lukert, P. D., 149Lund, Maryls E., 556Luoma, M. K., 21Lutticken, Doris, 533Lutticken, Rudolf, 533Lyons, Robert W., 582
McBride, Charles M., 14McGrath, M. B., 406McBride, Mollie E., 14McCloud, C. J., 364McGarrity, Gerard J., 264Macks, Gerald C., 421MacLowry, James D., 421Mardh, Per-Anders, 81Marks-Hellman, Sherry, 272Martin, William T., 486Marucha, P. T., 474Mason, T., 593Matsen, John M., 42, 105Mazens, Mary, 327Merritt, Katharine, 287
Merson, Michael H., 143, 486Merz, W. G., 496Michaud, R. N., 406Mihailescu, Cornelia, 211Millstein, C. H., 463Milner, E. C. B., 21Morris, George K., 143, 486Morse, C. Dwayne, 34Morse, Stephen A., 8Moroz, David A., 586Murphy, Brian R., 223Myers, Donald M., 548
Nakamura, Yoichi, 545Nebel, A. E., 26
Oberhofer, Thomas R., 137Oblack, Donna, 175Ogra, Pearay L., 469Ormsbee, R. A., 51Oriel, Christine A., 449Orr, Henry C., 402Otto, L. A., 566Owens, Dwight R., 218
Palutke, Waldemar A., 77Parisi, Joseph T., 519Parker, R. H., 599Parkinson, A. J., 637Patton, Charlotte M., 143Peacock, M. G., 51Pedersen, Carl E., Jr., 113, 460,
513Penner, John L., 385Peterson, Martin W., 376Petricciani, John C., 402Philip, R. N., 51Phillips, Betty J., 272Phillips, S. E., 291Pickett, M. J., 566Porschen, Richard K., 161Prince, Alfred M., 626Probst, Peter G., 402Pursell, Alfred R., 537
Qadri, S. M. Hussain, 102
Reinhardt, George F., 62Richman, Douglas D., 223Riddle, Conradine F., 206Roberts, Glenn D., 47, 157, 302Robertson, E. Arthur, 421Robinson, David M., 460Robrish, S. A., 474Rocha, Heonir, 67Rodr;guez, A., 26Rolfe, Rial D., 218Rottem, Shlomo, 110Rowen, Joyce W., 137Rubin, Sally J., 582Rudback, J. A., 21Ruitenberg, E. J., 541Russell, H., 501
Sack, David A., 486
Sack, R. Bradley, 486Sammons, L. S., 513Samuels, Sandra, 49Sandok, P. L., 72Sanford, G., 496Saphir, D. A., 344Saunders, George C., 604Savy, V. L., 26Saz, Arthur K., 330Schlossberg, David, 239Schneider, Lothar G., 96Schreckenberger, Paul C., 313Schryver, George D., 397Schwarz, Jan, 175Sechter, I., 390Segal, Herbert E., 465Selin, Merle J., 180Selva-Sutter, Ernesta A., 414Shepard, Maurice C., 613Shulman, Jonas A., 234Sielaff, Bruce H., 105Silcox, Vella A., 414Sivendra, R., 70Smart, Ross A., 376Smibert, R. M., 432Smith, John A., 5Smith, Rodney F., 640Smith, Thomas F., 334Sniderman, Steve, 77Socransky, S. S., 474Spendlove, Rex S., 376Staat, Robert H., 378Stalons, Donald R., 161Standard, Paul G., 191Stargel, M. D., 206, 291Steerenberg, P. A., 541Stevens, Robert J., 576Stotzky, Guenther, 119Strannegard, Oxian, 86Stretton, R. J., 635Sullivan-Sigler, Nadine, 133Sultz, Harry A., 469Sun, S. H., 186Syed, Salam A., 200
Taber, Larry H., 309Talbot, Henry W., Jr., 519Taylor, Welton I., 206Thirumoorthi, M. C., 318Thomas, K. R., 21Thompson, F. S., 291Thornsberry, C., 425Tiku, Moti L., 469Top, Franklin H., Jr., 465Treadwell, Thomas L., 287Triantafellu, N., 593Tu, Kevin K., 77Tzianabos, T., 609
Uhlendorf, Carol, 223
Valu, Joseph A., 172Vasil, Michael L., 382Vnek, John, 626Vukovich, K. R., 186
AUTHOR INDEX iii
Wang, Howard S., 302Wannamaker, Lewis W., 533Warren, Edward, 212Washington, John A., II, 212,
643Weaver, R. E., 425Weetall, Howard H., 402
Weissman, Jack B., 143Wells, Joy G., 486Wende, R. D., 102West, Susan E. H., 393Wheat, Robert, 545White, L. A., 609Wilkins, Tracy D., 359, 393
Wilkinson, Hazel W., 480Willhight, Milton, 465Williams, D. N., 556Williams, R. P., 102Wong, James, 640Wooley, R. E., 149
SUBJECT INDEX
VOLUME 3
Acetylmethylcarbinolproduced by N. gonorrhoeae, 34
Acute central nervous system infectionsviral antibody in, 397
Aerobic bacteriaschemata for identification, 161
Aeromonas hydrophilameningitis, 102
Affinity chromatographyelimination of bacteriophages
by, 402Agglutination test
false positive histoplasmin latex, 306serogrouping ofB. fragilis
subsp. fragilis, 586Anaerobes
survival in clinical specimens, 133Anaerobic bacteria
evaluation of schemata for detection of, 161in nonsupportive gassed transport system, 128modification of Minitek system to characterize,
291rapid fermentation testing of, 313
Anaerobic cell culture systemT. pallidum, 72
Antibodiesbronchitis virus, 149
Antigenhepatitis B surface, 626T, 593
API 20 C microtube systemto identify yeasts, 302
API 20S and API 20E kitsto identify Enterobacteriaceae, 421
Arcton 113to purify influenza virus, 524
ASO/ADB test kitevaluation of, 458
Autobac 1bacterial identification, 105
Bacillimethod for identification, 566
Bacteremiaeffect of volume of blood on, 643radiometric detection, 281
Bacteriaanaerobic, 161anaerobic, aerobic, 128effected by sonic treatment, 474tetrazolium reduction by, 327
Bacterial identificationcomputer-assisted, 105
Bacteriuriaassociation with bacteremia, 246
Bacteroides corrodensisolates of anaerobic gram-negative rods, 432
Bacteroides fragilisinhibition on blood agar plates, 359
Bacteroides fragilis subsp. fragilis
serogrouping by agglutination, 586Bacteroides melaninogenicus
description by fluorescent antibody staining, 506Bacti-Lab streptococci culture systems
to identify group A streptococci, 443Binary ethyleneimine
inactivation of viruses in serum with, 209Blood agar plates
inhibition of B. fragilis on, 359Blood cultures
Vibrio sp. isolated from, 425Blood culture technique
clinical evaluation, 251developmental phase, 258
Bronchitis virus antibodiesmicroneutralization test, 149
Brucella abortus antibodiesidentification of, 203
Cancermicrobial skin flora, 14
Candida agglutinin testin diagnosis of candidiasis, 175
Candidiasistests for diagnosis, 175
Catalase-negative strainS. aureus, 77
Ceratocystiscross-reactions with S. schenckii, 545
Cetylpyridinium chlorideeffect on fungi and N. asteroides in sputum, 272
Chagas' diseasedetection of antibodies, 67
Childrenanaerobic infections in, 318
Chlamydia subgroup Acomparison after incubation, 334
Choleracaused by enterotoxigenic E. coli, 382
Chromatographic detectionelectron capture gas, 34
Citrobacter diversusisolated from clinical material, 390
Clostridium chauvoeielectron capture gas chromatography study, 180
Clostridium septicumelectron capture gas chromatography study, 180
Coccidioides immitisidentification of, 186
Complement fixation testcompared with counterimmunoelectrophoresis
test, 67for histoplasmosis, 157
Conjugatesfluorescent antibody, 2
Coronavirusdetection by fluorescent virus precipitin test, 376
Cotton swab and scrub methodsfor differential quantitation of bacteria, 576
Counterimmunoelectrophoresisiv
SUBJECT INDEX v
compared with complement fixation test, 67of staphylococcal antibody, 599to recognize group B streptococci, 287
Cytomegalovirusdetection of antibodies, 364
Decarboxylasecompared with dihydrolase results, 75for differentiation of bacteria, 137
Dental plaque floramedium to detect gelatin-hydrolyzing activity,
200Diarrheamethods to detect E. coli, 486
Diarrhea virusinfant reovirus-like, 376neonatal calf, 376
Differential agar medium (A7)to identify U. urealyticum, 613
Dihydrolasecompared with decarboxylase results, 75for differentiation of bacteria, 137
Duodenal isolationof S. typhi, 456
Electron capture gas chromatography studyof C. septicum and C. chauvoei, 180
EnterobacteriaceaeAPI 20E and API 20S kits, 421unusual, 206
Enzyme-labeled antibody test, 604Equine encephalomyelitis viruses
preparation and testing of vaccines, 113Escherichia coli
as cause of clinical cholera, 382methods to detect, 486
Ethyleneimineviruses with, 209
Eubacterium lentumcontamination with S. sonnei, 206
Fluorescein-125I antibodyto detect virus-specific immunoglobulins, 637
Fluorescent antibody conjugatesstability of, 1
Fluorescent antibody stainingofB. melaninogenicus, 506of virus-infected cell cultures, 537
Fluorescent antibody testto diagnose gonorrhea, 438
Fluorescent virus precipitin testdetection of viruses, 375
Formate-fumerate energy metabolismB. corrodens, V. succinogenes, 432
Fungirecovery from clinical specimens, 47
Gas chromatographic characterizationof mycobacteria, 81
Gas chromatographyto detect cryptococcal meningitis, 239
Gelatin-hydrolyzing activityof human dental plaque flora, 200
Gentamicinclinical evaluation, 496
Germ tube dispersion testfor diagnosis of candidiasis, 175
Gingival florabacteria associated with dog bites, 344
Gloved-hand modelfor measurements of skin-degerming activity, 406
Gonorrheafluorescent antibody test, 438
Group B streptococcal typesIa, Tb, Ic, II, and III, 480
Group B streptococcirecognition by pigment production and counter-
immunoelectrophoresis, 287Gum tragacanth overlay
in plaque assay of viruses, 373
hardjo, from horses of Argentina, 548Hemadsorption inhibition method
filtration of Newcastle disease virus in, 227Hemolysis-in-gel test
for screening of rubella immunity, 86Hepatitis B surface antigen
in health care institution, 469persistence of antibody to, 465purification of, 626
Herpes simplex virus infectiondiagnosis by immunofluorescence, 309
Herpesvirus hominisuse of biological characteristics, 277
Hippurate hydrolysisby group B Streptococcus, 49
Histoplasmatest for identification of, 191
Histoplasmosiscomplement fixation tests, 157
Human diploid cell culturevaccine, 96
Immune globulinrabies fluorescein isothiocyanate-labeled, 609
Immunodiffusion testinfluenza virus A neuraminidase antigens, 233
Immunofluorescencediagnosis of herpes simplex virus infection, 309
Immunofluorescent identificationof mycoplasma colonies, 449
Immunological testto identify Histoplasma, 191
Immunoperoxidase techniqueto detect antibodies to cytomegalovirus, 364
Immunosorbent assayfor streptococcal M protein antibodies, 501measurement of rucroplites, 541
Indirect enzyme-labeled antibody conceptscreening for antibodies, 604
InfectionsShigella, 143
Influenza A virusinterferon sensitivity, 223neuraminidase antigens, 233
Influenza viruspurification by Arcton 113, 524
Isolation of anaerobeseffectiveness of palladium chloride for, 218
Leifson flagella strain
vi SUBJECT INDEX
simplified, 632Leptospira biflexa, 548
isolation from horses, 548Long-chain fatty acids
of Sporothrix (Sporotrichum) schenckii, 632
McCoy's cells5-iodo-2-deoxyuridine-treated, 334
Mediumfor detecting dental plaque flora, 200
Meningitisdiagnosis by gas chromatography, 239due to A. hydrophila, 102
Methanolysatestrifluoracetylated whole-cell, 81
Methodscotton swab and scrub, 576modified hemadsorption, 227Moeller, 75rapid, 75, 566replicator plate, 75simple disk-plate, 172
Microbial skin florafrom cancer patients, 14
Micrococcaceaecoagulase-negative, 556
Microimmunofluorescence testfor study of Rocky Mountain spotted fever, ty-
phus, 51Microneutralization test
to determine bronchitis virus antibodies, 149Microrespirator chamber
to determine viability, 560Microtiter test
cholesterol requirement of mycoplasmas, 110Minitek system
for characterization of anaerobic bacteria, 291to identify N. gonorrhoeae, 8
Mitis-Salivarius agarinhibition of S. mutans, 378
Modified hemadsorption methodtitration of Newcastle disease virus, 227
Mycobacteriagas chromatographic characterization of, 81
Mycobacteriological culturerecovery of fungi, 47
Mycobacteriumresembling M. fortuitum, 453
Mycobacterium fortuitumthat produces brown pigment, 453use of phage F-4WJ-1, 324
Mycobacterium gordonaesimple procedure for detection, 211
Mycobacterium szulgaidifferential identification of, 414
Mycobacterium tuberculosisdiscerning more phage types, 324
Mycological mediawith gentamicin added, 496
Mycoplasma coloniesimmunofluorescent identification, 449
Mycoplasmascholesterol requirement of, 110
Neisseriasimple disk-plate method, 172
Neisseria gonorrhoeaeacetylmethylcarbinol produced by, 34identification with Minitek system, 8production of acid from glucose, 330
Neutralization testinfluenza virus A neuraminidase antigens, 233
Newcastle disease virusand its neutralizing antibodies, 227
Nocardia sp.use of C02, 463
Nocardia asteroideseffect of cetylpyridinium chloride on, 272
Nonsupportive gassed transport systemanaerobic and aerobic bacteria, 128
Palladium chlorideeffectiveness of, 218
Peptostreptococcus anaerobiusinhibition of, 393
Phage typingof S. epidermidis, 519
Phage typing setfor salmonellae, 214
Plaque assayof Sendai virus, 71
Precipitin testfor diagnose Candidiasis, 175
Procedureto detect M. gordonae, 211
Prophylactic immunizationof humans against rabies, 96
Proteus rettgeri 0-serotyping systemevaluation of, 385
Pseudomonas aerginosaepidemiology of infections, 264
Pseudomonas cepaciafrom water reservoirs, 62
Pseudomonas stutzeripitting of agar surface by, 381
Pyocen typingdetermining P. aerginosa infections, 264
Rabiesfluorescein isothiocyanate, 609prophylactic immunization, 96
Radioimmunoassayfor measuring group B streptococcal types, 480
Radiometric detectionof bacteremia, 281
Rapid decarboxylase testfor differentiation of bacteria, 137
Rapid fermentation testingof anaerobic bacteria, 313
Rhamnose-positive Y. enterocoliticabiochemical characteristics, 119
Rheumatoid factorblocking effect, 157
Rhinovirusisolation of, 528
Rocky Mountain spotted feverantibody response to, 513microimmunofluorescence test, 51
Rubella hemagglutination inhibitionevaluation of, 5
Salmonella
method for identifying, 339Salmonellaegroup Cl and C2, 214
Salmonella typhiduodenal isolation of, 456
Screening of rubella immunityhemolysis-in-gel-test, 86
Sendai virusplaque assay, 91
Serotype hardjofrom horses, 548
Serratia marcescens
serotyping and biotyping of, 582Shigellamethod for identifying, 339
Shigella infectionsseroepidemiology of, 143
Shigella sonneicontamination with E. lentum, 206
Simian virus 40-induced T-antigen expression, 593
Simple disk plate methodfor confirmation of Neisseria, 172
Single radial diffusionto identify B. abortus, 203
Skin-degerming activitygloved-hand model, 406
Sodium amylosulfatecompared with sodium polyanetholsulfonate, 212
Sodium polyanetholsulfonatecompared with sodium amylsulfate, 212P. anaerobius inhibition by, 393
Sonic treatmenteffect on bacteria, 474
Sporothrix schenckiicross-reactions with Ceratocystis, 545
Sporothrix (Sporotrichum) schenckiilong-chain fatty acids of, 635
Sporotrichotic patientsdelayed cross-reactions in, 545
Staphylococcal antibodycounterimmunoelectrophoresis of, 599
Staphylococcus aureus
catalase-negative strain, 77Staphylococcus epidermidisphage typing of, 519
Streptococcal M antibodiesmeasured by radioimmunoassay, 501
StreptococciBacti-Lab streptococci culture systems, 443
Streptococcus (group B)determination of Hippurate hydrolysis, 49
Streptococcus mutansinhibition by Mitis-Salivarius agar, 378
Streptococcus pyogenesnew method to detect M types of, 533
Techniqueblood culture, 251, 258immunoperoxidase, 364
Testagglutin, 175
SUBJECT INDEX vii
agglutination, 586complement fixation, 67counterimmunoelectrophoresis, 67decarboxylase, 75, 137dihydrolase, 75, 137enzyme-labeled antibody, 604fluorescent antibody, 438germ tube dispersion, 175hemolysis-in-gel, 86immunodiffusion, 227microimmunofluorescence, 51microtiter, 110neutralization, 233precipitin, 175rubella hemagglutination inhibition, 5to determine Hippurate hydrolysis, 49
Tetrazolium reductionby bacteria, 327
Treponema pallidumin an aerobic cell culture system, 72
Tuberculosisfalse positive histoplasmin latex agglutination
test, 306Typhusmicroimmunofluorescence test, 51
Ureaplasma urealyticumdifferential agar medium (A7) to identify, 613
Vaccinehuman diploid cell culture, 96prepared from equine encephalomyelitis viruses,
113Venezuelan equine encephalomyelitis, 460
Vaginal yeastsisolation on media, 640
Venezuelan equine encephalomyelitis vaccineimproved method for production of, 460
Vibriohalophilic, 425
Vibrio succinogenesisolates of anaerobic gram-negative rods, 432
Viral antibodyin acute central nervous system infections, 397
Virushuman cytomegalovirus, 364influenza A, 223Newcastle disease, 227Sendai, 91
Viruses in plaque assayuse of gum tragacanth overlay, 373
Virus neutralization testto determine bronchitis virus antibodies, 147
Virus-specific immunoglobulinsdetection of, 637
Yeastsidentified by API 20C microtube system, 302vaginal, 640
Yersinia enterocoliticarhamnose-positive, 119
INDEX TO DATE OF ISSUE
Month Date of Issue Pages
January 6 February 1976 1-80February 2 March 1976 81-221March 24 March 1976 223-384April 30 April 1976 385-464May 25 May 1976 465-543June 2 July 1976 545-646
JOURNALOF
MICROBIOLOGY
VOLUME 3
WASHINGTON, D.C. 20006
1976
JOURNAL OF CLINICAL MICROBIOLOGYVOLUME 3 1976
EDITORIAL BOARDALBERT BALOWS, Editor-in-Chief (1979)
Center for Disease Control,Atlanta, Ga.
LORRAINE FRIEDMAN, Editor (1979)Tulane University Medical School,
New Orleans, La.
HENRY D. ISENBERG, Editor (1979)Long Island Jewish-Hillside Medical Center,
New Hyde Park, N. Y.NATHALIE J. SCHMIDT, Editor (1980)
California Department of Health,Berkeley, Calif
Daniel Amsterdam (1977)Libero Ajello (1976)William B. Cherry (1978)Walter R. Dowdle (1976)V. R. Dowell, Jr. (1977)Richard W. Emmons (1977)John C. Feeley (1917)James D. Fenters (1976)Sydney M. Finegold (1976)Maxwell Finland (1977)Herman Friedman (1978)Thomas L. Gavan (1977)Morris A. Gordon (1978)Dieter Groschel (1977)Leonor D. Haley (1977)James L. Hardy (1978)Melvin T. Hatch (1978)Harry R. Hill (1977)
Lillian V. Holdeman (1976)Milton Huppert (1978)S. S. Kalter (1978)Leo Kaufman (1977)George E. Kenny (1976)George P. Kubica (1976)Howard W. Larsh (1977)James D. MacLowry (1977)Harold Markowitz (1977)William J. Martin (1977)John M. Matsen (1977)Joseph L. Melnick (1976)William I. Metzger (1977)Maurice A. Mufson (1977)Barbara G. Painter (1977)Demosthenes Pappagianis (1977)Robert H. Purcell (1977)William E. Rawls (1977)
Glenn D. Roberts (1977)Jon E. Rosenblatt (1977)Margarita Silva-Hutner (1977)James W. Smith (1977)Louis DS. Smith (1978)Peter B. Smith (1976)Alex C. Sonnenwirth (1977)Welton I. Taylor (1977)John H. Thompson, Jr. (1977)Richard C. Tilton (1978)Marvin Turck (1977)Alexander von Graevenitz (1977)Kenneth W. Walls (1977)Lewis W. Wannamaker (1978)Robert E. Weaver (1978)Irene Weitzman (1978)Robert P. Williams (1978)Marion E. Wilson (1977)
Robert A. Day, Managing EditorGisella Pollock, Director of Editorial Services
Leslie Frymire, Production Editor1913 I St., N. W., Washington, D.C. 20006
EX OFFICIOHelen R. Whiteley, President (1975-1976) Harlyn 0. Halvorson, Vice-President (1975-1976)
J. Mehsen Joseph, Secretary Brinton M. Miller, Treasurer
The Journal of Clinical Microbiology, a publication of theAmerican Society for Microbiology, 1913 I St., N. W., Wash-ington, D.C. 20006, is devoted to the dissemination of newknowledge concerning the applied microbiological aspects ofhuman and animal infections and infestations, particularlyregarding their etiologic agents, diagnosis, and epidemiology.Papers dealing with antibiotics and antimicrobial agents andchemotherapy, with fundamental aspects of infection andimmunity, and with food or dairy microbiology fall within thescope of other ASM publications. The Journal is publishedmonthly, and the twelve numbers are divided into two volumesper year. The nonmember subscription price is $50 per year.The member subscription price is $13 per year. Single copiesare $5. Correspondence relating to subscription, reprints,
defective copies, availability of back issues, lost or late proofs,disposition of submitted manuscripts, and general editorialmatters should be directed to the ASM Publications Office,1913 I St., N.W., Washington, D.C. 20006 (area 202833-9680).
Second-class postage paid at Washington, D.C. 20006, and atadditional mailing offices.Made in the United States of America.Copyright © 1976, American Society for Microbiology.1976, American Society for Microbiology.All Rights Reserved.
Volume 3 Contents for January Number I
BacteriologyAdaptation of the Minitek System for the Rapid Identification of Neisseria
gonorrhoeae. STEPHEN A. MORSE* AND LYNNE BARTENSTEIN .... .......... 8Electron Capture Gas Chromatographic Detection of Acetylmethylcarbinol Pro-
duced by Neisseria gonorrhoeae. C. DWAYNE MORSE, JOHN B. BROOKS,* ANDDOUGLAS S. KELLOGG, JR . .............................................. 34
Evaluation of an Adenosine 5'-Triphosphate Assay as a Screening Method toDetect Significant Bacteriuria. DAVID N. ALEXANDER, GRACE M. EDERER,AND JOHN M. MATSEN* ............. .................................... 42
Rapid, Colorimetric Test for the Determination of Hippurate Hydrolysis by GroupB Streptococcus. STEPHEN C. EDBERG* AND SANDRA SAMUELS .... ........ 49
Unusual Chromobacterium violaceum: Aerogenic Strains. R. SIVENDRA ....... 70Retention of Motility of Treponema pallidum (Nichols Virulent Strain) in an An-
aerobic Cell Culture System and in a Cell-Free System. P. L. SANDOK,*H. M. JENKIN, S. R. GRAVES, AND S. T. KNIGHT .......................... 72
Comparison of Amino Acid Decarboxylase and Dihydrolase Results by Moeller,Rapid, and Replicator Plate Methods. RONALD N. JONES,* PETER C.FUCHS, AND STEVE SNIDERMAN .......................................... 75
Isolation and Characterization of a Catalase-Negative Strain of Staphylococcusaureus. KEVIN K. Tu* AND WALDEMAR A. PALUTKE ..... ................ 77
Virology, Rickettsiology, and ChlamydiologyEvaluation of a Rubella Hemagglutination Inhibition Test System.
JOHN A. SMITH* AND A. C. CUMMINS .................................... 5
Ethylenimine-Inactivated Rabies Vaccine of Tissue Culture Origin. 0. P.LARGHI, V. L. SAVY, A. E. NEBEL, AND A. RODRIGUEZ* ..... .............. 26
Microimmunofluorescence Test for the Serological Study of Rocky MountainSpotted Fever and Typhus. R. N. PHILIP,* E. A. CASPER, R. A. ORMSBEE,M. G. PEACOCK, AND W. BURGDORFER ................................... 51
MycologyRecovery of Pathogenic Fungi from Clinical Specimens Submitted for Myco-
bacteriological Culture. GLENN D. ROBERTS,* ALFRED G. KARLSON, ANDDONALD R. DEYOUNG .............. .................................... 47
Nosocomial InfectionsMicrobial Skin Flora of Selected Cancer Patients and Hospital Personnel.
MOLLIE E. McBRIDE,* W. CHRISTOPHER DUNCAN, GERALD P. BODEY, ANDCHARLES M. MCBRIDE .................................................. 14
Pseudomonas cepacia Strains Isolated from Water Reservoirs of Unheated Nebu-lizers. SHELDON M. GELBART,* GEORGE F. REINHARDT, AND HERBERT B.GREENLEE ............................................................. 62
ParasitologyComparison of the Complement Fixation Test and Counterelectrophoresis Test
for the Detection of Antibodies in Chagas Disease. RALPH A. KNIGHT,*HEONIR ROCHA, AND DONALD KAYE ........ .............................. 67
Clinical Laboratory ImmunologyStability of Fluorescent Antibody Conjugates Stored Under Various Conditions.* Asterisk refers to person to whom inquiries regarding the paper should be addressed.
xi
JAMES H. GREEN, SAMUEL B. GRAY, JR., AND W. KNOX HARRELL* .1Preparation and Properties of a National Reference Endotoxin. J. A. RuD-
BACH,* F. I. AKIYA, R. J. ELIN, H. D. HOCHSTEIN, M. K. LUOMA, E. C. B.MILNER, K. C. MILNER, AND K. R. THOMAS .............................. 21
ERRATUMIndirect Hemagglutination Test for Human Antibody to Typhus and Spotted
Fever Group Rickettsiae. AKIRA SHIRAI, JOHN W. DIETEL, AND JOSEPHV. OSTERMAN*........................................................ 79
Volume 3 Contents for February Number 2Bacteriology
Gas Chromatographic Characterization of Mycobacteria: Analysis of Fatty Acidsand Trifluoroacetylated Whole-Cell Methanolysates. LENNART LARSSON*AND PER-ANDERS MARDH ........... .................................... 81
Meningitis Due to Aeromonas hydrophila. S. M. HUSSAIN QADRI,* L. P. GORDON,R. D. WENDE, AND R. P. WILLIAMS ........ .............................. 102
Computer-Assisted Bacterial Identification Utilizing Antimicrobial SusceptibilityProfiles Generated by Autobac 1. BRUCE H. SIELAFF,* EUGENE A. JOHNSON,AND JOHN M. MATSEN ............. .................................... 105
Temperature-Dependent Cultural and Biochemical Characteristics of Rhamnose-Positive Yersinia enterocolitica. BRENT CHESTER* AND GUENTHER STOTZKY . 119
Survival of Anaerobic and Aerobic Bacteria in a Nonsupportive Gassed TransportSystem. ANTHONY W. CHOW,* PATRICK J. CUNNINGHAM, AND LUCIEN B.GUZE ................................................................. 128
Anaerobes Survive in Clinical Specimens Despite Delayed Processing. JOHN G.BARTLETT,* NADINE SULLIVAN-SIGLER, THOMAS J. LOUIE, AND SHERWOOD L.GORBACH ..............................................................133
Evaluation ofthe Rapid Decarboxylase and Dihydrolase Test for the Differentiationof Nonfermentative Bacteria. THOMAS R. OBERHOFER,* JOYCE W. ROWEN,JAMES W. HIGBEE, AND RONALD W. JOHNS ...... ........................ 137
Evaluation of Simplified Dichotomous Schemata for the Identification of AnaerobicBacteria from Clinical Material. RICHARD K. PORSCHEN* AND DONALD R.STALONS .............................................................. 161
Simple Disk-Plate Method for the Biochemical Confirmation of Pathogenic Neis-seria. JOSEPH A. VALU ............ .................................... 172
Electron Capture Gas Chromatography Study of the Acid and Alcohol Products ofClostridium septicum and Clostridium chauvoei. JOHN B. BROOKS,* MERLE J.SELIN, AND CYNTHIA C. ALLEY ......... ................................. 180
A New Medium for the Detection of Gelatin-Hydrolyzing Activity of Human Den-tal Plaque Flora. SALAM A. SYED ........ .............................. 200
Unusual Enterobacteriaceae: H2S+ Shigella sonnei, One Authentic and One FalsePositive Due to Contamination with the Obligate Anaerobe Eubacteriumlentum. J. J. FARMER III,* CONRADINE F. RIDDLE, M. D. STARGEL, H. IIDA,T. AIKAWA, DAVID ACHANZAR, AND WELTON I. TAYLOR ...... .............. 206
Simple Procedure for Detection of Mycobacterium gordonae in Water CausingFalse-Positive Acid-Fast Smears. DELIA DIZON, CORNELIA MIHAILESCU, ANDH. C. BAE* ........................................................... 211
Comparison of Sodium Amylosulfate and Sodium Polyanetholsulfonate in Blood
xii CONTENTS
Culture Media. M. MARSHA HALL, EDWARD WARREN, DUANE M. ILSTRUP,AND JOHN A. WASHINGTON II* ......... ................................. 212
Effectiveness of Palladium Chloride for the Isolation of Anaerobes. DWIGHT R.OWENS,* RIAL D. ROLFE, AND DAVID J. HENTGES ...... ................... 218
Virology, Rickettsiology, and ChlamydiologyEvaluation of the Hemolysis-in-Gel Test for the Screening of Rubella Immunity
and the Demonstration of Recent Infection. LENA GRILLNER* AND ORJANSTRANNEGARD......................................................... 86
Plaque Assay of Sendai Virus in Monolayers of a Clonal Line of Porcine KidneyCells. H6MU ITO...................................................... 91
Prophylactic Immunization ofHumans Against Rabies by Intradermal Inoculationof Human Diploid Cell Culture Vaccine. JAMES H. Cox* AND LOTHAR G.SCHNEIDER ............................................................. 96
Preparation and Testing of Vaccines Prepared from the Envelopes of Venezuelan,Eastern, and Western Equine Encephalomyelitis Viruses. CARL E. PEDER-SEN, JR . .............................................................. 113
Comparison ofa Microneutralization Test in Cell Culture and Virus NeutralizationTest in Embryonated Eggs for Determining Infectious Bronchitis Virus Anti-bodies. R. E. WOOLEY,* J. BROWN, R. B. DAVIS, J. L. BLUE, AND P. D.LUKERT ............................................................... 149
Inactivation of Viruses in Serum with Binary Ethyleneimine. HANs G. BAHNE-MANN ................................................................ 209
MycologyBlocking Effect of Rheumatoid Factor and Cold Agglutinins on Complement Fixa-
tion Tests for Histoplasmosis. JAMES E. JOHNSON AND GLENN D. ROBERTS . 157Comparative Evaluation of the Candida Agglutinin Test, Precipitin Test, and
Germ Tube Dispersion Test in the Diagnosis of Candidiasis. DONNA OBLACK,JAN SCHWARZ, AND IAN A. HOLDER* ..................................... 175
Rapid In Vitro Conversion and Identification of Coccidioides immitis. S. H. SUN,M. HUPPERT,* AND K. R. VUKOVICH ..................................... 186
Specific Immunological Test for the Rapid Identification of Members of the GenusHistoplasma. PAUL G. STANDARD* AND LEO KAUFMAN ..... .............. 191
ImmunologyIdentification ofBrucella abortus Antibodies in Cattle Serum by Single Radial Dif-
fusion. DOMENICO IANNELLI,* RAMON DIAZ, AND T. MANLIO BETTINI ...... 203
EpidemiologyDiagnostic Value of Indirect Hemagglutination in the Seroepidemiology ofShigella
Infections. CHARLOTTE M. PArrON,* EUGENE J. GANGAROSA, JACK B. WEISS-MAN, MICHAEL H. MERSON, AND GEORGE K. MORRIS ..... ................. 142
Phage Typing Set for Group Cl and C2 Salmonellae. M. GERSHMAN .... ....... 214
MycoplasmatologyCholesterol Requirement of Mycoplasmas as Determined by a Microtiter Test
Using Polyene Antibiotics. MARION W. GRABOWSKI,* SHLOMO ROTTEM, ANDMICHAEL F. BERILE..................................................... 110
ERRATAPolypectate Digestion by Yersinia. V. LYLE VON RIESEN ..... ................ 221Convenient, Simplified Preparation of Less Commonly Used Media. V. LYLE
VON RIESEN ........................................................... 221
CONTENTS *x.ii
Improved Transport System for Neisseria gonorrhoeae in Clinical Specimens.DOROTHY A. SYMINGTON................................................. 1
Volume 3 Contents for March Number3Bacteriology
Polymicrobic Bacteriuria: Significant Association with Bacteremia. PETER A.GROSS,* MARGARET FLOWER, AND GERTRUDE BARDEN ...................... 246
Blood Culture Technique Based on Centrifugation: Developmental Phase. GOR-DON L. DORN,* JOHN R. HAYNES AND G. GENE BURSON .................... 251
Blood Culture Technique Based on Centrifugation: Clinical Evaluation. GORDONL. DORN,* G. GENE BURSON, AND JOHN R. HAYNES....................... 258
Clinical Comparison of Aerobic, Hypertonic, and Anaerobic Culture Media for theRadiometric Detection of Bacteremia. R. MARIE COLEMAN, W. WAYNELASLIE, AND D. W. LAMBE, JR.*...... ................................... 281
Rapid Recognition of Group B Streptococci by Pigment Production and Counter-immunoelectrophoresis. KATHARINE MERRITT,* THOMAS L. TREADWELL, ANDNICHOLAS J. JACOBS................................................... 287
Modification of the Minitek Miniaturized Differentiation System for Characteriza-tion of Anaerobic Bacteria. M. D. STARGEL,* F. S. THOMPSON, S. E. PHILLIPS,G. L. LOMBARD, AND V. R. DOWELL, JR ................................. 291
Rapid Fermentation Testing of Anaerobic Bacteria. PAUL C. SCHRECKENBERGER*AND DONNA J. BLAZEVIC............................................... 313
Anaerobic Infections in Children: a Prospective Survey. M. C. THIRUMOORTHI,BARBARA M. KEEN, AND ADNAN S. DAJANI .............................. 318
Use of Phage F-OWJ-1 of Mycobacterium fortuitum to Discern More Phage Typesof Mycobacterium tuberculosis. W. D. JONES, JR.,* AND J. GREENBERG..... 324
Acceleration of Tetrazolium Reduction by Bacteria. RAYMOND C. BARTLETT,MARY MAZENS,* AND BRIAN GREENFIELD ................................. 327
Effect of Types of Media on the Production of Acid from Glucose by So-CalledGlucose-Negative Strains of Neisseria gonorrhoeae. ELLEN S. BARON ANDARTHUR K. SAZ*....................................................... 330
Method for Identifying Salmonella and Shigella Directly from the Primary Isola-tion Plate by Coagglutination of Protein A-Containing Staphylococci Sen-sitized with Specific Antibody. EARL A. EDWARDS* AND RICHARD L.HILDERBRAND.......................................................... 339
Gingival Flora of the Dog with Special Reference to Bacteria Associated withBites. D. A. SAPHIR AND G. R. CARTER* ................................ 344
Differentiation of Proteus mirabilis by Bacteriophage Typing and the Dienes Re-action. F. W. HICKMAN AND J. J. FARMER III* ..... ..................... 350
Inhibition of Bacteroides fragilis on Blood Agar Plates and Reversal of Inhibitionby Added Hemin. TRACY D. WILKINS,* SARAH L. CHALGREN, F. JIMENEZ-ULATE, C. R. DRAKE, JR., AND J. L. JOHNSON ..... ....................... 359
Inhibition of Streptococcus mutans Strains by Different Mitis-Salivarius AgarPreparations. ROBERT H. STAAT ........ ............................... 378
Pitting of Agar Surface by Pseudomonas stutzeri. JANE J. ALEXANDER AND JAYF. LEWIS* ............................................................ 381
Clinical Cholera Caused by Enterotoxigenic Escherichia coli. RICHARD A.FINKELSTEIN,* MICHAEL L. VASIL, JAMES R. JONES, RICHARD A. ANDERSON,AND TOM BARNARD..................................................... 382
XIV CONTENTS
Virology, Rickettsiology, and ChlamydiologyThree Strains of Influenza A Virus (H3N2): Interferon Sensitivity In Vitro and
Interferon Production in Volunteers. DOUGLAS D. RICHMAN, BRIAN R. MUR-PHY,* SAMUEL BARON, AND CAROL UHLENDORF ...... ..................... 223
Titration of Newcastle Disease Virus and Its Neutralizing Antibodies in Micro-plates by a Modified Hemadsorption and Hemadsorption Inhibition Method.DAVID KATZ,* HANA BEN-MOSHE, AND SHLOMIT ALON ..... ................ 227
Antigenic Relationships Among Influenza Virus A Neuraminidase (N2) Antigensby Immunodiffusion and Postinfection Neutralization Test. WALTER R.DOWDLE,* W. GRAEME LAVER, JUDITH C. GALPHIN, AND JEAN C. DOWNIE .. 233
Use of Biological Characteristics to Type Herpesvirus hominis Types 1 and 2 inDiagnostic Laboratories. SHERRY MARKS-HELLMAN AND MONTO Ho* ...... 277
Diagnosis of Herpes Simplex Virus Infection by Immunofluorescence. LARRYH. TABER,* FRANCOISE BRASIER, ROBERT B. COUCH, STEPHEN B. GREENBERG,DAN JONES, AND VERNON KNIGHT ........ ............................... 309
Comparison of Chlamydia Subgroup A Detection from Clinical Specimens After40 and 64 Hours of Incubation in 5-Iodo-2-Deoxyuridine-Treated McCoy'sCells. JAMES E. JOHNSON* AND THOMAS F. SMITH ..... .................. 334
Immunoperoxidase Technique for Detection of Antibodies to Human Cytomegalo-virus. G. GERNA,* C. J. MCCLOUD, AND R. W. CHAMBERS ..... ........... 364
Use of Gum Tragacanth Overlay, Applied at Room Temperature, in the PlaqueAssay of Fish and Other Animal Viruses. PETER DOBOS ..... ............ 373
Detection of Neonatal Calf Diarrhea Virus, Infant Reovirus-Like Diarrhea Virus,and a Coronavirus Using the Fluorescent Virus Precipitin Test. MARTINW. PETERSON, REX S. SPENDLOVE,* AND Ross A. SMART ..... .............. 376
MycologyPossibility of Diagnosing Meningitis by Gas Chromatography: Cryptococcal
Meningitis. DAVID SCHLOSSBERG, JOHN B. BROOKS,* AND JONAS A. SHULMAN 239
Effect of Cetylpyridinium Chloride on Pathogenic Fungi and Nocardia asteroidesin Sputum. BETTY J. PHILLIPS* AND WILLIAM KAPLAN ..... .............. 272
Evaluation of the API 20 C Microtube System for the Identification of ClinicallyImportant Yeasts. GLENN D. ROBERTS,* HOWARD S. WANG, AND GARY E.HOLLICK .............................................................. 302
Apparent False Positive Histoplasmin Latex Agglutination Test in Patients withTuberculosis. ARTHUR F. DISALVO* AND DOROTHY S. CORBETT .... ........ 306
EpidemiologyEpidemiology of Pseudomonas aeruginosa Infections: Determination by Pyocin
Typing. JOHAN N. BRUUN, GERARD J. MCGARRITY,* WILLIAM S. BLAKEMORE,AND LEWIS L. CORIELL ............. .................................... 264
Volume 3 Contents for April Number 4Bacteriology
Citrobacter diversus Isolated from Clinical Material. G. ALTMANN, I. SECHTER,*D. CAHAN, AND CH. B. GERICHTER ...................................... 390
Medium-Dependent Inhibition of Peptostreptococcus anaerobius by Sodium Poly-anetholsulfonate in Blood Culture Media. TRACY D. WILKINS* AND SUSAN E.H. WEST .............................................................. 393
Differential Identification of Mycobacterium szulgai and Other Scotochromogenic
CONTENTS xv
Mycobacteria. ERNESTA A. SELVA-SUTTER, VELLA A. SILCOX,* AND HUGO L.DAVID ................................................................ 414
Analysis of Cost and Accuracy of Alternative Strategies for EnterobacteriaceaeIdentification. E. ARTHUR ROBERTSON,* GERALD C. MACKS, AND JAMES D.MACLOWRY ............................................................ 421
Halophilic Vibrio Species Isolated From Blood Cultures. D. G. HOLLIS,* R. E.WEAVER, C. N. BAKER, AND C. THORNSBERRY ............................ 425
Clinical Isolates of Anaerobic Gram-Negative Rods with a Formate-FumerateEnergy Metabolism: Bacteroides corrodens, Vibrio succinogenes, and Uniden-tified Strains. R. M. SMIBERT* AND LILLIAN V. HOLDEMAN .... ........... 432
Evaluation of the Bacti-Lab Streptococci Culture Systems for Selective Recoveryand Identification of Group A Streptococci. PAT W. FREEBURG* AND JUDY M.BUCKINGHAM.......................................................... 443
Mycobacterium Resembling Mycobacterium fortuitum That Produces Brown Pig-ment. JEAN E. HAWKINS* AND ELEANORA B. FALCO ..... ................ 453
Duodenal Isolation ofSalmonella typhi by String Capsule in Acute Typhoid Fever.ROBERT H. GILMAN* AND RICHARD B. HORNICK ...... ..................... 456
Use of CO2 for More Rapid Growth of the Nocardia Species. L. H. BONCYK,* C. H.MILLSTEIN, AND S. S. KALTER .......... ................................. 463
Virology, Rickettsiology, and ChlamydiologyViral Antibody in the Cerebrospinal Fluid of Patients with Acute Central Nervous
System Infections. RUDOLF DEIBEL AND GEORGE D. SCHRYVER .... ........ 397Elimination of Bacteriophages from Tissue Culture Serum by Affinity Chromatog-
raphy. HENRY C. ORR,* HOWARD H. WEETALL, PETER G. PROBST, DEWITT C.LITTLEJOHN, FRED C. CHU, JOYCE B. JOHNSON, AND JOHN C. PETRICCIANI ... 402
Improved Method for Production of Attenuated Venezuelan Equine Encepha-lomyelitis (TC-83 Strain) Vaccine. FRANCIS E. COLE, JR.,* CARL E. PEDER-SEN, JR., DAVID M. ROBINSON, AND GERALD A. EDDY ..... ................. 460
ImmunologyFluorescent Antibody Test for the Serological Diagnosis of Gonorrhea. HASSAN
A. GAAFAR* AND DORA C. D'A.RCANGELIS ................................ 438Simple Method for Immunofluorescent Identification of Mycoplasma Colonies.
JANET M. BRADBURY,* CHRISTINE A. ORIEL, AND F. T. W. JORDAN .... ..... 449Evaluation of the Beckman Antistreptolysin 0 and Antideoxyribonuclease B
(ASO/ADB) Test Kit. GEORGE C. KLEIN ................................ 458
EpidemiologyEvaluation of a Proteus rettgeri O-Serotyping System for Epidemiological Investi-
gation. JOHN L. PENNER,* NORMAN A. HINTON, AND JOAN N. HENNESSY .. 385
Nosocomial InfectionsApplication of a Gloved-Hand Model for Multiparameter Measurements of Skin-
Degerming Activity. R. N. MICHAUD,* M. B. MCGRATH, AND W. A. Goss . 406
Volume 3 Contents for May Number5
BacteriologyEffect of Sonic Treatment on Pure Cultures and Aggregates of Bacteria. S. A.
XVi CONTENTS
ROBRISH,* S. B. GROVE, R. S. BERNSTEIN, P. T. MARUCHA, S. S. SOCRANSKY,AND B. AMDUR......................................................... 474
Description of a Polyvalent Conjugate and a New Serogroup of Bacteriodesmelaninogenicus by Fluorescent Antibody Staining. DWIGHT W. LAMBE,JR.,* AND ROBERT C. JERRIS ......... ................................... 506
Application of a New Method for Detecting Streptococcal Nicotinamide AdenineDinucleotide Glycohydrolase to Various M Types of Streptococcus pyogenes.RuDoLF LUTTIcKEN,* DORIS LuTTrIcKEN, DWIGHT R. JOHNSON, AND LEWIS W.WANNAMAKER........................................................ 533
Virology, Rickettsiology, and ChlamydiologyPersistence of Antibody to Hepatitis B Surface Antigen. GILBERT R. IRWIN,*
ALFRED M. ALLEN, HERBERT E. SEGAL, MILTON WILLHIGHT, HUBERT CANNON,AND FRANKLIN H. Top, JR ............................................. 465
Hepatitis B Infection in Health Care Personnel of an Institution for MentallyHandicapped Children and Adults. MOTI L. TIKU, KARL R. BEUTNER,PATRICIA CARMODY, HARRY A. SULTZ, BANSI L. KAUL, AND PEARAY L. OGRA* 469
Antibody Response to Rocky Mountain Spotted Fever. R. H. KENYON,* P. G.CANONICO, L. S. SAMMONS, L. R. BAGLEY, AND C. E. PEDERSEN, JR......... 513
Purification of Influenza Virus with Arcton 113. MARGARET I. BRADY* AND I. G.S. FURMINGER ......................................................... 524
Comparison of Rhinovirus-Sensitive HeLa Cells and Human Embryo Fibroblastsfor Isolation of Rhinoviruses from Patients with Respiratory Disease. F. A.LEWIS* AND MARGERY L. KENNETT ...................................... 528
Procedure for Fluorescent-Antibody Staining of Virus-Infected Cell Cultures inPlastic Plates. ALFRED R. PURSELL* AND JOHN R. COLE, JR .............. 537
ImmunologyRadioimmunoassay for Measuring Antibodies Specific for Group B Streptococcal
Types Ia, Ib, Ic, II, and III. HAZEL W. WILKINSON* AND WALLIS L. JONES.. 480Enzyme-Linked Immunosorbent Assay for Streptococcal M Protein Antibodies.
H. RUSSELL,* R. R. FACKLAM, AND L. R. EDWARDS ........................ 501Direct Measurement of Microplates and Its Application to Enzyme-Linked Im-
munosorbent Assay. E. J. RUITENBERG,* B. J. M. BROSI, AND P. A.STEERENBERG......................................................... 541
EpidemiologyLaboratory Investigation of Diarrhea in Travelers to Mexico: Evaluation of
Methods for Detecting Enterotoxigenic Escherichia coli. GEORGE K. MOR-RIS,* MICHAEL H. MERSON, DAVID A. SACK, Joy G. WELLS, WILLIAM T.MARTIN, WALLIS E. DEWITT, JOHN C. FEELEY, R. BRADLEY SACK, AND DAVIDM. BESSUDO ........................................................... 486
Phage Typing of Staphylococcus epidermidis. HENRY W. TALBOT, JR., ANDJOSEPH R. PARISI*..................................................... 519
MycologyClinical Evaluation of the Addition of Gentamicin to Commercially Prepared
Mycological Media. W. G. MERZ, G. SANFORD, AND G. L. EVANS .......... 496
ERRATAPhage Typing Set for Group C, and C2 Salmonellae. M. GERSHMAN .... ...... 543Clinical Comparison of Aerobic, Hypertonic, and Anaerobic Culture Media for the
Radiometric Detection of Bacteremia. R. MARIE COLEMAN, W. WAYNELASLIE, AND D. W. LAMBE, JR.* ........ ................................ 543
x*iiCONTENTS
Volume 3 Contents for June Number 6Bacteriology
Serological Studies and Isolations of Serotype hardjo and Leptospira biflexaStrains from Horses of Argentina. DONALD M. MYERS ..... .............. 548
Significance of Urinary Isolates of Coagulase-Negative Micrococcaceae. D. N.WILLIAMS,* MARYLS E. LUND, AND DONNA J. BLAZEVIC ..... .............. 556
Rapid Method for Identification of Gram-Negative, Nonfermentative Bacilli. L.A. OTTO AND M. J. PICKETT* ............................................ 566
A Simplified Leifson Flagella Stain. W. A. CLARK .......................... 632Effect of Volume of Blood Cultured on Detection of Bacteremia. M. MARSHA
HALL, DUANE M. ILSTRUP, AND JOHN A. WASHINGTON II* ...... ........... 643
Virology, Rickettsiology, and ChlamydiologySystematic Variables Affecting Simian Virus 40-Induced T-Antigen Expression
and Transformation in Human Cells. M. M. KAPLAN, A. S. LUBINIECKI,*W. A. BLArrNER, T. MASON, D. J. GIARD, M. GuNNELL, N. TRIANTAFELLU, ANDJ. F. FRAUMENI, JR. .............................................. 593
Preparation of Rabies Fluorescein Isothiocyanate-Labeled Immune Globulin fromMouse Hyperimmune Ascitic Fluids. T. TZIANABOS,* G. A. HEBERT, ANDL. A. WHITE ............. ................................ 609
Large-Scale Purification of Hepatitis B Surface Antigen. JOHN VNEK AND AL-FRED M. PRINCE* .................... ......................... 626
Detection of Virus-Specific Immunoglobulins Using a Doubly Labeled Fluorescein-125I Antibody. A. J. PARKINSON* AND J. KALMAKOFF ....... .............. 637
ImmunologyCounterimmunoelectrophoresis of Staphylococcal Antibody. J. FEDORKO, B. E.
FOSSIECK, JR.,* AND R. H. PARKER ................ ...................... 599Rapid Micromethod of Screening for Antibodies to Disease Agents Using the
Indirect Enzyme-Labeled Antibody Concept. GEORGE C. SAUNDERS* ANDELVA H. CLINARD .................... ......................... 604
EpidemiologyCombined Serotyping and Biotyping of Serratia marcescens. SALLY J. RUBIN,*
SUSAN BROCK, MARY CHAMBERLAND, AND ROBERT W. LYONS ..... .......... 582Serogrouping of Bacteroides fragilis subsp. fragilis by the Agglutination Test.
DWIGHT W. LAMBE, JR.,* AND DAVID A. MOROZ ........ .................. 586
MycologyDelayed Hypersensitivity Cross-Reactions Between Sporothrix schenckii and
Ceratocystis Species in Sporotrichotic Patients. HIROSHI ISHIZAKI,* YOICHINAKAMURA, HIDEO KARIYA, TOKIO IWATSU, AND ROBERT WHEAT .... ....... 545
Long-Chain Fatty Acids of Sporothrix (Sporotrichum) schenckii. R. J. STRETTONAND R. K. DART*....................................................... 632
Comparative Isolation of Vaginal Yeasts on Selective and Nonselective Media.MARY D. BRASHEAR, JAMES WONG, PAULA HINES, AND RODNEY F. SMITH* .. 640
Nosocomial InfectionsDifferential Quantitation of Surface and Subsurface Bacteria of Normal Skin by
the Combined Use of the Cotton Swab and the Scrub Methods. CHARLESA. EVANS* AND ROBERT J. STEVENS ........ ............................. 576
xviii CONTENTS
CONTENTS xix
MycoplasmatologyMicrorespirometer Chamber for Determinations of Viability in Cell and Organ
Cultures. MICHAEL G. GABRIDGE ....................................... 560Differential Agar Medium (A7) for Identification of Ureaplasma urealyticum
(Human T Mycoplasmas) in Primary Cultures of Clinical Material. MAU-RICE C. SHEPARD* AND CARL D. LUNCEFORD .............................. 613
ERRATAEffects of Types of Media on the Production of Acid from Glucose by So-Called
Glucose-Negative Strains of Neisseria gonorrhoeae. ELLEN S. BARON* ANDARTHUR K. SAZ........................................................ 646
Rapid In Vitro Conversion and Identification ofCoccidioides immitis. S. H. SUN,M. HUPPERT,* AND K. R. VUKOVICH ..................................... 646