Objective SWBAT identify the process of transcription as it
relates to The Central Dogma by accurately transcribing 13/15 DNA
sequences into mRNA
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Central Dogma of molecular biology
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Central Dogma of molecular biology Main or important Belief or
principle Main Belief The flow of genetic information in cells is
from __DNA___ to __RNA__ to __protein______. It involves 2
steps:transcription &translation
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Transcription DNARNAPROTEIN Central Dogma of molecular biology
Central Dogma part 1
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DNA RNA ribosomes endoplasmic reticulum vesicle Golgi apparatus
vesicle protein on its way! protein finished protein Making
Proteins TO: nucleus
What is Transcription? Comes from the word _________________ To
transcribe something means to _______ it in another way. transcribe
copy transcription In love Knockin on heavens door Like a rolling
stone All along the water tower
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Why is Transcription Important? Why do we need to make a new
copy? Help, I cant get out! Ribosome DNA cannot Leave the
__________. How can a message get to the ribosome? ___________ mRNA
Nucleus m = Messenger
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Transcription: making a copy
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Step 1: RNA Helicase ________ DNA unwinds TATA TATA
AGCTCGAACGGA TCCGTTCGAGCT A G C T C G AA C G G A T C C GTTCG A G C
T 3 5 3 RNA Helicase
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Transcription: making a copy Step 2: RNA Polymerase
______nucleotides to mRNA in the 5 3 direction adds TATA TATA A G C
T C G AA C G G A T C C GTTCG A G C T 3 5 3 UCCGUUCGAGC RNA
polymerase mRNA Exception to base-pair rule: ___ instead of ___
UT
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Practice: What is the complementary DNA strand? 1.Template: 5
TACT 3 1.Template: 3 GCAT 5
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*Step 2A: There are useless DNA codes called INTRONS that get
spliced out. The pieces that are left, called EXONS, form the
actual mRNA. Pre-mRNA: UCCGGAGUAAC AGUGGA intron exon GAGUAA mRNA:
UCCGC Exons are expressed! DNA RNA = Transcription
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Transcription: making a copy Step 3: DNA _________ to double
helix and mRNA ________ the nucleus returns TATA TATA A G C T C G
AA C G G A 3 5 3 UCCGUUCGAGC mRNA leaves T C C GTTCG A G C T
AGCTCGAACGGA TCCGTTCGAGCT
Slide 16
DNA RNA ribosomes endoplasmic reticulum vesicle Golgi apparatus
vesicle protein on its way! protein finished protein Making
Proteins TO: nucleus
Slide 17
Video: Transcription Summary
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PRACTICE DNA Strand 1: ATA CGA CAT AGG AAC TAT CAG mRNA Strand:
DNA Strand 2: AAA GGG CCC GAT AGA ACC GAA mRNA Strand: DNA Strand
3: GGG CCC GAT AGG GAA AAT GAG mRNA Strand: DNA Strand 4: GGA TAC
GAT CAT ATA CCA ATC mRNA Strand:
