Special Abstracts / Journal of Biotechnology 150S (2010) S1–S576 S239

Reference

Li, X., Ma, H., Wang, Q., Matsumoto, S., Maeda, T., Ogawa, H.I., 2009. Isolation, identifi-cation of sludge-lysing strain and its utilization in thermophilic aerobic digestionfor waste acticated sludge. Bioresource Technology 100 (9), 2475–2481.

doi:10.1016/j.jbiotec.2010.09.093

[P-E.72]

Norovirus contamination in shellfish from seven harvestingareas in the Yellow Sea, South Korea

M Kwon 1, W.C. Choi 1, C Lee 1, H.-J. Lee 2, I Kang 1, S Cheong 1, S.W.Song 1, E.-G. Oh 2, H.-S. Yu 2, S.-B. Shin 2, S.-J. Kim 1,∗

1 Seoul National University, Republic of Korea2 National Fisheries Research & Development Institute, Republic ofKoreaKeywords: norovirus; shellfish; reverse transcription-nested PCR

Noroviruses (NoV) are the major cause of epidemic acute gas-troenteritis, and shellfish harvested from NoV-contaminated seaareas is considered as significant vectors of viral transmission. Weperformed reverse transcription-nested PCR (RT-nested PCR) toassess norovirus contamination in Korean shellfish. From Februaryto November 2007, shellfish samples were collected monthly fromseven different shellfish production sea areas in the Yellow Sea.The samples were concentrated, viral RNAs were extracted, andnoroviral presence was determined by RT-nested PCR, followed bysequence analysis of the amplicons. Of 70 shellfish samples, six-teen (22.9%) samples were positive for NoV genogroup II (NoVGII). NoV were frequently detected from spring to summer andtheir occurrence was different along sample types and collectingsites. By sequence analysis, all the NoV amplicons were closelyrelated to NoV GII/4 strains which cause worldwide outbreaks. Ourresults indicated that monitoring NoV contamination in shellfishfood could be useful to assess NoV-associated outbreaks from theconsumption of Korean shellfish.

doi:10.1016/j.jbiotec.2010.09.094

[P-E.73]

Novel derivatives of thiazolidinedione as selective algicidalagents against harmful algal bloom

Hoon Cho 1,4, EonSeon Jin 2, Thi Minh Hang 1, Si Wouk Kim 3,∗

1 Department of Polymer Science & Engineering, Chosun University,Republic of Korea2 Department of Life Science and Research Institute for Natural Sci-ences, Hanyang University, Republic of Korea3 Department of Environmental Engineering, Chosun University,Republic of Korea4 Research Center for Resistant Cells, Chosun University, Republic ofKoreaKeywords: Harmful algal bloom; thiazolidinediones; Cochlodiniumpolykrikoides; algicidal activity

This work reported the synthesis of 28 thiazolidinedione (TD)derivatives having algicidal activity against microalgae causingharmful algal bloom (HAB). Among 28 compounds tested, mostshowed highly effective algicidal activity against Cochlodiniumpolykrikoides and Chattonella marina, however, they were relativelytolerant to non-harmful algae. Compounds 6, 13, 22 and 26 werethe most potent against C. polykrikoides with IC50 values < 0.5 �M.

Interestingly, compounds 7, 13, 27 and 28 were selective to C.polykrikoides with IC50 values ranging from 0.1 to 2 �M, but theyare not sensitive to C. marina and H. akashiwo. The growth of C.marina was inhibited by compound 2 with IC50 value < 0.5 �M. Ourresults provided strong evidences that several TD derivatives haveeffective algicidal activity against HABs but are safe to non harmfulalgae.

This work was supported by the Pioneer Research Programthrough the National Research Program of Korea funded bythe Ministry of Education, Science and Technology (Grant No.M1071118001-08M1118-00110).

doi:10.1016/j.jbiotec.2010.09.095

[P-E.74]

Simultaneous decolorization of mixed dye wastewater and elec-tricity generation using a two chambered microbial fuel cell

M.H. Cho 1,∗, S. Kalathil 1, S.-H. Shim 1,2, Y.H. Lee 2, J. Lee 1

1 Yeungnam University, Republic of Korea2 Institute of Metabolic Engineering Co., Republic of KoreaKeywords: Microbial fuel cell; Power generation; Dye; Decoloriza-tion Wastewater treatment

Various dyes are commonly used in the textile industry. Thesedyes needed to be removed from the effluent prior to dischargeto sewage due to their intense colour and toxicity. In this studywe used two chambered microbial fuel cell, separated by a pro-ton exchange membrane with mixed real dye wastewater in anodechamber and 50 mM buffer solution at cathode with air supplyusing an air pump for simultaneous decolorization and electric-ity production. As we used mixed dye wastewater, we didn’t useany co-substrate like glucose for electricity generation. Usually, azodyes were cleaved biotically at the anode while electricity was pro-duced from readily biodegradable organic substrates present in themixed dye wastewater. A voltage of 0.3 V (40mW/m2) was obtainedwhile decolorization efficiency was 62% after 8 days of the MFCoperation. The slow decolorization efficiency may be due to theinhibition kinetics of various dyes present in the dye wastewaterand so it is pivotal to study the inhibition kinetics of different dyes.Also we have been investigating simultaneous aerobic-anaerobictreatment of the dye wastewater using biocathode microbial fuelcell by using the same mixed dye wastewater at the anode andcathode.

doi:10.1016/j.jbiotec.2010.09.096

[P-E.75]

Multiple community states of planktonic communities on func-tional gene structure in microbial electrolysis cell (MEC)

Aijie Wang 1,∗, Wenzong Liu 1, Ye Deng 2,3, Joy D. Van Nostrand JoyD. Van Nostrand 2,3, Jizhong Zhou 2,3

1 State Key Lab of Urban Water Resource and Environment, Harbin,China2 Institute for Environmental Genomics, Stephenson Research & Tech-nology Center, University of Oklahoma, Norman, United States3 Virtual Institute for Microbial Stress and Survival, United StatesKeywords: Planktonic microbial communities; functional gene;Microbial electrolysis cell; H2 production

Microbial electrolysis cells (MEC) show promise for efficientrecovery of hydrogen and methane from waste using exoelectro-