North Carolina State University Oat Maker

Development

Peter MaloneyJeanette LyerlyPaul Murphy

Gina Brown-Guedira USDA-ARS

Crop Science Department840 Method Rd, Unit 3

Raleigh, NC 27695

Winter Hardiness ProjectGoal: To develop lines with increased tolerance to cold and

freezing temperatures and map Winter Hardiness Component Traits (WHCT)

Preliminary Work– Screened known Oat RFLP and SSR markers in Fulghum x

Norline RIP phenotyped for WHCT– Tested Wheat SSR primers for amplification in Oat

• Markers were randomly chosen and had a very low success rate• One oat SSR developed through this screening is associated with a

QTL for WHCT

Winter Hardiness ProjectSSR markers

• Collaborating with Dr. Joe Anderson’s lab at Purdue University to test newly developed SSR markers

• Evaluated primers generated by the Anderson lab on a panel of 11 different cultivars

– Used the Licor 4300 Analysis System

• Those that were polymorphic for Fulghum x Norline RIP were then mapped onto the population

– **See poster by Peter Maloney for more information**

Winter Hardiness ProjectSNP discovery

• Sequence PCR fragments• First SNP developed into CAPS1 marker

– See Linkage Group FN7 for location*

• Develop SNP markers from oat ESTs• Herman et al. (2006) described regions in wheat associated with

up or down-regulation during cold acclimation• ESTs from these genes were used to develop markers that were

screened for amplification in our panel of oat cultivars– Through this process 2 SNPs and 1 deletion were found with a 4%

success rate

(* See poster by Peter Maloney)

Future Plans• Develop cDNA libraries using oats that have

been exposed to sub-zero temperatures• Develop RIL to study BYDV resistance in oats

• Associate markers with BYDV resistance for marker assisted selection

• Develop RIL to study Grain Protein Content• Identify markers that can be used in marker assisted

selection for Grain Protein