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NORTH CAROLINA STATE UNIVERSITY GRADUATE COURSE ACTION FORM
DEPARTMENT/PROGRAM
COURSE PREFIX/NUMBER
PREVIOUS PREFIX/NUMBER
DATE OF LAST ACTION
COURSE TITLE
ABBREVIATED TITLE
SCHEDULING Fall Spring Summer Every Year Alt. Year Odd Alt. Year Even Other COURSE OFFERED BY DISTANCE EDUCATION ONLY ON CAMPUS ONLY
BOTH ON CAMPUS AND BY DISTANCE EDUCATION CREDIT HOURS
CONTACT HOURS Lecture/Recitation Seminar Laboratory Problem Studio Independent Study/Research Internship/Practicum/Field Work
GRADING ABCDF S/U
INSTRUCTOR (NAME/RANK) Graduate Faculty Status Associate Full
ANTICIPATED ENROLLMENT Per semester Max.Section Multiple sections Yes No
PREREQUISITE(S)
COREQUISITE(S)
PRE/COREQUISITE FOR
RESTRICTIVE STATEMENT
CURRICULA/MINORS Required Qualified Elective
PROPOSED EFFECTIVE DATE APPROVED EFFECTIVE DATE __________________________
CATALOG DESCRIPTION (limit to 80 words):
RECOMMENDED BY: _______________________________________________________________________ Department Head/Director of Graduate Programs Date ENDORSED BY: _______________________________________________________________________ Chair, College Graduate Studies Committee Date ________________________________________________________________________ College Dean(s) Date APPROVED: _______________________________________________________________________
Dean of the Graduate School Date
TYPE OF PROPOSAL New Course Drop Course
Course Revision Dual-Level Course
REVISION Content Prefix/Number Title
Abbreviated Title Credit Hours
Contact Hours Grading Method
Pre-Corequisites Restrictive Statement
Description Scheduling
DOCUMENTATION AS REQUIRED Please number all document pages
Course Justification
Proposed Revision(s) with Justification
Student Learning Objectives
Enrollment for Last 5 Years
New Resources Statement
Consultation with other Departments
Syllabus (Old and New)
Explanation of differences in requirements of dual-level courses
Population Health and Pathobiology Update Course Information Title: Biological Light and Electron Microscopy: Principles and Practice Coordinator: Dr. Michael J. Dykstra, PHP Department Prefix & Number: CBS 732 Scheduled: Fall, every other year beginning in 2005 Course Justification: This course was created in 1984, and its purpose was to “…provide veterinary medical science graduate students with the skills and knowledge to examine materials by transmission and scanning electron microscopy. Electron microscopy techniques specifically geared to animal research as it relates to physiology, anatomy, pathology, microbiology and clinical applications are not offered anywhere else on campus.” This has been a course concerned with the application of techniques to solve cell biology problems. A single published paper review session was added the last time the course was taught. It was so successful an effort, both in the eyes of the instructor and the students, that it is proposed to have three lecture periods devoted to the review of published papers in the future, which requires more lecture periods than exist in the current course design. Additionally, placing the revised course format in the fall would be more applicable for the instructor’s goals for the course. Additionally, the national society devoted to electron microscopy changed its name from the Electron Microscopy Society of America to the Microscopy Society of America in 1992 to embrace all forms of microscopy, including confocal scanning microscopy, light microscopy, scanning tunneling microscopy, etc. The course has slowly been changing to mirror this broadened base in the biological microscopy community, and the revised course would have significant additions in the areas of light microscopy, confocal scanning microscopy, and digital imaging. Which necessitates significant numbers of added lecture material. Also, each of the other technologies involved in imaging that have been discussed over the last 20 years have had numerous increases in complexity and new approaches, which have made the lecture schedule quite crowded for the last 7-10 years the course has been taught. During the twenty (20) years the course has been taught, a much wider variety of students from the College of Agriculture and Life Sciences and the College of Textiles has joined the originally exclusive College of Veterinary Medicine student population in the course. The course has slowly evolved into a general biologically-oriented electron microscopy course, which evolved to a depressing of the specific veterinary medicine focus of the course. So it has become appropriate to adapt the course to accommodate the broader biological bent of the course. Compared to its original narrower format, a changed course period, course length as well as course name will successfully bridge these needs. Enrollment: The average enrollment in this course since the first time it was taught in 1984 to the spring of 2005 has been 5.4 students, with a range from 3 to 8. With the broaden base of the course for fall semester, expected enrollment is 8 – 10.
Population Health and Pathobiology Resources Required: There are no additional resources needed. Consultation with other Departments: The only other electron microscopy facility on campus that serves the biological community is the CALS facility managed by Dr. John Mackenzie. The existing course was set up with a mission that did not conflict with his offerings in 1985 and I believe the revised course description will not conflict with his current offerings (see attached letter from Dr. Mackenzie). Student Learning Objectives: Students will be able to work with preparative techniques for scanning electron microscopy, transmission electron microscopy, and light microscopy, and confocal scanning laser microscopy. Production of digital images by light microscopy as well as limited use of Adobe Photoshop to prepare images for Powerpoint presentations and publication in printed form will be examined. Finally, methods for morphometric analysis, utilizing Image-ProPlus will be explored. Go to our website (www.cvm.ncsu.edu/research/laelom) to see examples of images and techniques that will be covered in the course. Students completing this course will have learned basic principles for fixing and embedding biological materials for electron microscopy. They will be able to evaluate the suitability of a given preparative regimen for their research. They will have gained familiarity with enzymatic cytochemistry, non-enzymatic cytochemistry, and immunocytochemistry. They will have learned the purpose and procedures involved in cryopreservation of biological samples. Students will produce negative stain preparations of particulate samples utilizing film-coated grids which they have prepared. Students completing this course will have learned how to operate a transmission electron microscope, a scanning electron microscope, several ultramicrotomes, a vacuum evaporator, a critical point dryer, and a sputter coater. Darkroom techniques and digital imaging techniques, that will be learned, will include negative development, print making, design and assembly of materials for publication, Powerpoint presentations, and poster design. Students will be introduced to the principles of light microscopy utilizing different optical systems and will have the opportunity to have hands-on experience with the Olympus VANOX photomicroscope as well as the Nikon C-1 confocal laser scanning microscope in conjunction with a Nikon Eclipse 2000E motorized inverted microscope. They will also be instructed concerning the applications and capabilities of morphometry, digitizing/archiving programs, and telemedicine capabilities available today. Students will be made aware of the availability and uses for high voltage electron microscopy, intermediate voltage electron microscopy, electron tomography, field emission scanning electron microscopy, vacuum systems, electron optics, low vacuum scanning electron microscopy, environmental scanning electron microscopy, various light microscopy optical systems, and confocal microscopy.
December 21, 2004 Memorandum To: Dr. Michael J. Dykstra Research Professor & LAELOM Director Veterinary Medicine From: Dr. John M. Mackenzie, Jr. Coordinator, Center for Electron Microscopy Professor, Microbiology CALS Subject: Course I have reviewed the proposed changes to your course. I see no conflict with the courses in electron microscopy in the College of Agriculture and Life Sciences. JMM:dc
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Biological Light and Electron Microscopy: Principles and Practice CBS 732
Course Syllabus
Instructor and Course Coordinator: Michael J. Dykstra C-108 513-6202 [email protected] Goals and Objectives: This course is designed for students to learn about the basic light and electron optical methods that may be utilized for cytological evaluation of biological samples. To such a degree that they can help define structure-function relationships within cells, thus being able to relate biochemical, molecular, physiological, and visual information to better understand cellular behavior. Student Learning Objectives: Students will be able to work with preparative techniques for scanning electron microscopy, transmission electron microscopy, and light microscopy, and confocal scanning laser microscopy. Production of digital images by light microscopy as well as limited use of Adobe Photoshop to prepare images for Powerpoint presentations and publication in printed form will be examined. Finally, methods for morphometric analysis, utilizing Image-ProPlus will be explored. Go to our website (www.cvm.ncsu.edu/research/laelom) to see examples of images and techniques that will be covered in the course. Students completing this course will have learned basic principles for fixing and embedding biological materials for electron microscopy. They will be able to evaluate the suitability of a given preparative regimen for their research. They will have gained familiarity with enzymatic cytochemistry, non-enzymatic cytochemistry, and immunocytochemistry. They will have learned the purpose and procedures involved in cryopreservation of biological samples. Students will produce negative stain preparations of particulate samples utilizing film-coated grids which they have prepared Students completing this course will have learned how to operate a transmission electron microscope, a scanning electron microscope, several ultramicrotomes, a vacuum evaporator, a critical point dryer, and a sputter coater. Darkroom techniques and digital imaging techniques, that will be learned, will include negative development, print making, design and assembly of materials for publication, Powerpoint presentations, and poster design. Students will be introduced to the principles of light microscopy utilizing different optical systems and will have the opportunity to have hands-on experience with the Olympus VANOX photomicroscope as well as the Nikon C-1 confocal laser scanning microscope in conjunction with a Nikon Eclipse 2000E motorized inverted microscope. They will also be instructed concerning the applications and capabilities of morphometry, digitizing/archiving programs, and telemedicine capabilities available today. Students will be made aware of the availability and uses for high voltage electron microscopy, intermediate voltage electron microscopy, electron tomography, field emission scanning electron microscopy, vacuum systems, electron optics, low vacuum scanning electron microscopy, environmental scanning electron microscopy, various light microscopy optical systems, and confocal microscopy.
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Textbook and Course Material: Suggested Textbook: Dykstra, M.J., and Reuss, L.E. 2003. Biological Electron Microscopy: Theory, Techniques, and Troubleshooting. 2nd Edition. Kluwer Academic/Plenum Publishers, New York. 534 p. -or- Bozzola, J.J., and Russell, L.D. 1999. Electron Microscopy: Principles and Techniques for Biologists. 2nd Edition. Jones and Bartlett Publishers, Boston. 670 p. Student Manual (provided by instructor at no cost): Dykstra, M.J. 2004. Electron microscopy in Veterinary Medicine. 84 p. Course Organization: The course is designed to be a five (5) hour course. This allows more time to discuss materials. The lecture and laboratory topics are listed in the course outline below. The course lectures hours will have some of the lecture sessions devoted to manuscript reviews to evaluate scientific approaches used, applicability of techniques, clarity of description of materials and methods used (for reproducibility), and quality of images created. The laboratories will be hands-on applications of the techniques discussed in lectures. This a five (5) credit graduate level course. There will be three fifty (50) minute lectures a week and two, two (2)-hour laboratories a week. The Special Projects labs are designed for students to finish their technical work that leads to the production of the laboratory hand-in that constitutes half of the grade for the course. The course objectives will be presented and covered through a combination of lectures, guided discussions, manuscript reviews, and laboratory exercises. Review of Manuscripts: Discussions of published literature will be used to highlight concepts considered during lectures. Personal Laboratory Project as Part of Laboratory Hand-in: Before the third week of class, each student will discuss a potential personal project with the instructor, which shall consist of two tissue or cell samples to be compared in some fashion, utilizing transmission electron microscopy techniques and ultramicrotomy. The results of this project, along with the other laboratory exercises, will be written up in scientific style, presented orally to the rest of the class, and the final write-up will be due the last day of classes.
Grading: The grading scale will be A to F. The grade will be comprised of: A+ 98-100 C 78-80.9 A 95-97.9 C- 76-77.9 A- 92-94.9 D+ 73-75.9 B+ 89-91.9 D 70-72.9 B 86-88.9 D- 68-69.9 B- 84-85.9 F <68 C+ 81-83.9
1. Two mid-term exams (25%) 2. A final exam (25%) 3. A Laboratory Hand-in (50%)
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Miscellaneous: This course is open to graduate students, staff, and faculty with a biology background. Permission of instructor is required so that students can be apprised of the effort required before they commit themselves. Attendance at all classes is expected, but justified absences can be made up by students working more in the laboratory to catch up, on their own schedule. The instructor will make every effort to help students who have unavoidable absences catch up through personal instruction or referral to reading material that covers the topics missed. No incompletes are given in this course and all laboratory hand-in material is due at the time of the final exam. ACADEMIC INTEGRITY: It is expected that both the students and instructor will abide by the University policy on academic integrity found in the Code of Student Conduct (http://www.ncsu.edu/policies/student_services/student_discipline/POL1.35.1.php. DISABILITY STATEMENT: Any student that may have a disability that may interfere with his/her work performance in the course is expected to notify the instructor during the initial conference to obtain permission to take the course. This will allow the College and/or instructor enough time to develop teaching aids or course modifications to accommodate the handicapped student’s needs. Any concerns about these issues can be answered by the Student Services Office or instructor prior to the course (http://www.ncsu.edu/provost/offices/affirm_action/dss/). Before any students are allowed to work in the laboratory, they are trained according to the Safety Plan for the LAELOM that is updated yearly. After the training, they sign certifications to that effect and are continually updated on safety issues during the term of the course. The only expense to be incurred by students beyond the optional textbook purchases will be a set of jeweler’s forceps, at a price from approximately $10-20.
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Course Outline:
Light and Electron Microscopy: Principles and Practice CBS-732
Lecture 9:10-10:00 am Monday, Wednesday, and Friday Lab 2:35-4:25 pm Tuesday and Thursday Office hours 8:30-9:30 am Tuesday and Thursday Instructor: Dr. Michael J. Dykstra, C-108; 513-6202 Texts: Dykstra, M.J., and Reuss, L.E. 2003. Biological Electron Microscopy: Theory, Techniques, and Troubleshooting. 2nd Edition. Kluwer Academic/Plenum Publishers, New York. 534 p. -or- Bozzola, J.J., and Russell, L.D. 1999. Electron Microscopy: Principles and Techniques for Biologists. 2nd Edition. Jones and Bartlett Publishers, Boston. 670 p. Student Manual (provided by instructor at no cost): Dykstra, M.J. 2004. Electron microscopy in Veterinary Medicine. 84 p. Course Outline: Lecture # Topic 1 Course Introduction 2 Principles of Fixation
3 Fixation, Dehydration, Embedding of Tissues 4 Embedding Media, Infiltration, Polymerization 5 Support Films 6 Review of Published Papers, Preparative Techniques 7 Ultramicrotome Design 8 Ultramicrotomy and Knife Making 9 Ultramicrotomy 10 Historical Review of Electron Microscopy 11 Theory of the Electron Microscope 12 Electron Emission, Electron Optics 13 Electron Optics, Condensor Lenses
14 Electron Optics, Objective Lens, Image Formation and Apertures 15 Hourly Exam #2 16 Electron Optics, Projector Lenses, Viewing Screens and Cameras 17 Electron Optics, Projector Lenses, Viewing Screens and Cameras 18 Vacuum Systems
19 Capturing Images on Film and Producing Silver-Based Prints 20 Replica Techniques 21 Particulate Specimens, Negative Staining and Shadow Casting 22 Cryotechniques 23 Cryotechniques 24 Scanning Electron Microscopy 25 Scanning Electron Microscopy
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26 Scanning Electron Microscopy 27 Review of Published Papers, Cryotechniques and SEM 28 Intermediate and High Voltage Electron Microscopy 29 Electron Tomography 30 Microprobe Analysis 31 Microprobe Analysis, STEM, Dark Field Imaging 32 Cytochemistry 33 Cytochemistry 34 Immunocytochemistry 35 Light Microscopy 36 Light Microscopy
37 Digital Imaging/Telemedicine 38 Confocal Scanning Microscopy 39 Confocal Scanning Microscopy 40 Confocal Scanning Microscopy 41 Review of Published Papers, Light and Confocal Scanning Microscopy 42 Morphometry 43 Summation Course Outline: Lab Number Topic 1 Orientation to the Laboratory, Assemble Kits 2 Fixation and Tissue Embedment 3 Block Trimming, Glass Knives
4 Glass Knives, Semithin Sectioning 5 Microtomy 6 Microtomy (Ultrathin Sections) 7 Section Staining, Support Films, Section Pick-up 8 Introduction to the Philips/FEICO EM208S 9 Introduction to the EM208S, Continued 10 Stigmation, Through-Focus Series 11 Negative and Print Processing 12 Wehnelt Assembly, Column Alignment 13 SEM Tissue Preparation 14 Critique 15 SEM Operation in Conventional Secondary Mode
16 SEM, Backscatter Mode, Low Vacuum Mode, Poor-Man’s Cryo 17 Light Microscopy
18 Light Microscopy 19 Digital Image Production and Adobe Photoshop Use 20 Confocal Laser Scanning Microscopy 21 Confocal Laser Scanning Microscopy 22 Digital Graphics 23 Digital Graphics 24 Introduction to Morphometry 25 Special Projects 26 Special Projects 27 Special Projects 28 Special Projects 29 Final Critique
5 Year Enrollment Statistics for CBS 732
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Semester Section Total Students Total Credit Hrs Total
SPRG '00 1 6 24
Total: 1 6 24
