Nikon Ti E service manual

R-4820.A

2004 NIKON CORPORATIONC

All rights reserved

Repair Manual

NIKON CORPORATION

ECLIPSE TI-E Inverted Research Microscope

R4820.A

R-4819 0 REV. RECORD R-4820 REV. NUMBER: 0

PAGE REV.MARK REV. DATE PAGE REV.MARK REV. DATE PAGE REV.MARK REV. DATE1 A 46 A 91 A 2 47 A 92 A 3 A 48 A 93 A 4 A 49 A 94 A 5 A 50 A 95 A 6 A 51 A 96 A 7 A 52 A 97 A 8 A 53 A 98 A 9 A 54 A 99 A

10 A 55 A 100 A 11 A 56 A 101 A 12 A 57 A 102 A 13 A 58 A 103 A 14 A 59 A 104 A 15 A 60 A 105 A 16 A 61 A 106 A 17 A 62 A 107 A 18 A 63 A 108 A 19 A 64 A 109 A 20 A 65 A 110 A 21 A 66 A 111 A 22 A 67 A 112 A 23 A 68 A 113 A 24 A 69 A 114 A 25 A 70 A 115 A 26 A 71 A 116 A 27 A 72 A 117 A 28 A 73 A 118 A 29 A 74 A 119 A 30 A 75 A 120 A 31 A 76 A 121 A 32 A 77 A 122 A 33 A 78 A 123 A 34 A 79 A 124 A 35 A 80 A 125 A 36 A 81 A 126 A 37 A 82 A 127 A 38 A 83 A 128 A 39 A 84 A 129 A 40 A 85 A 130 A 41 A 86 A 131 A 42 A 87 A 132 A 43 A 88 A 133 A 44 A 89 A 134 A 45 A 90 A 135 A

R4820.A

Overview ...........................................................................................................................................P.1

Points on Repair ................................................................................................................................P.1

1. Tools..................................................................................................................................................P.2

1-1. Tools for General Use and Preparations ....................................................................................P.2

1-2. Tools for Special Purposes.........................................................................................................P.3

2. Specifications ....................................................................................................................................P.8

2-1. Specifications .............................................................................................................................P.8

3. Schematic Diagram of the Optical System ........................................................................................P.9

3-1. Schematic Diagram of the Ti-E Optical System..........................................................................P.9

3-2. Components in the Optical System ..........................................................................................P.10

4. Connection Diagram........................................................................................................................ P.11

4-1. Inner Connection Diagram of Ti-E ............................................................................................ P.11

5. Removing/Installing the Optical Unit................................................................................................P.12

5-1. Removing the Accessories .......................................................................................................P.12

5-2. Removing the Optical Unit........................................................................................................P.13

5-3. Installing the Optical Unit..........................................................................................................P.17

6. Disassembling/Assembling the Up-Down Movement Unit...............................................................P.18

6-1. Disassembly.............................................................................................................................P.18

6-2. Assembly..................................................................................................................................P.26

7. Disassembling/Assembling the Joint Gears ....................................................................................P.29

7-1. Disassembly.............................................................................................................................P.30

7-2. Assembly..................................................................................................................................P.35

8. Disassembling/Assembling the Coaxial Coarse/Fine Focus Knob ..................................................P.36

8-1. Disassembling the Coaxial Coarse/Fine Focus Knob ..............................................................P.37

8-2. Assembling the Coaxial Coarse/Fine Focus Knob ...................................................................P.43

9. Disassembling/Assembling the Second Objective Lens..................................................................P.44

9-1. Disassembling the Second Objective Lens ..............................................................................P.45

9-2. Assembling the Second Objective Lens...................................................................................P.47

10. Disassembling/Assembling the Optical Path Change-Over Block ...................................................P.49

10-1. Disassembling the Optical Path Change-Over Block ...............................................................P.50

10-2. Assembling the Optical Path Change-Over Block ....................................................................P.52

Contents

R4820.A

11. Optical Adjustment ..........................................................................................................................P.53

11-1. Preparation for Optical Adjustment...........................................................................................P.53

11-2. Adjustment of Stop Position of Turret .......................................................................................P.57

11-3. L100 (Prism in Left Observation Optical System) Optical Axis Adjustment ..............................P.59

11-4. Focusing and Optical Axis Adjustment on the Objective Side ..................................................P.60

11-4-1. Preparation............................................................................................................................P.60

11-4-2. Adjustment of the Optical Axis and Parfocality of the 1x Second Objective Lens..................P.62

11-4-3. Adjustment of the Optical Axis and Parfocality of the 1.5x Second Objective Lens...............P.63

11-5. Preparations Common to Overall Optical Adjustments.............................................................P.64

11-5-1. Setting...................................................................................................................................P.64

11-5-2. Temporary Adjustment of Focus and Optical Axis Adjustment...............................................P.65

11-5-3. Focusing ...............................................................................................................................P.66

12. Overall Optical Adjustment ..............................................................................................................P.68

12-1. Focusing and Optical Axis Adjustment on the Observation System .........................................P.68

12-1-1. Preparation ...........................................................................................................................P.68

12-1-2. Focusing and Optical Axis Adjustment ..................................................................................P.69

12-1-3. Beam Adjustment..................................................................................................................P.70

12-2. Focusing and Optical Axis Adjustment on the Side Port...........................................................P.71

12-2-1. Preparation (for focusing and optical axis adjustment on the right- and left-side ports) ........P.71

12-2-2. Optical Axis Adjustment ........................................................................................................P.72

12-2-3. Focusing Adjustment.............................................................................................................P.73

13. Electrical Adjustments .....................................................................................................................P.75

13-1. Encoder Adjustment .................................................................................................................P.75

13-1-1. Preparation ...........................................................................................................................P.75

13-1-2. Adjustment of Tilt of Encoder Head.......................................................................................P.76

13-1-3. Amplitude (Gain) Adjustment of the Preamplifier Board ........................................................P.78

13-1-4. Center Level (Offset) Adjustment of the Preamplifier Board .................................................P.79

13-1-5. Confirmation of Lissajous Waveform ....................................................................................P.80

13-1-6. Signal Specifications.............................................................................................................P.81

13-2. Upper- and Lower-Limit Sensor Adjustment.............................................................................P.82

13-2-1. Checking Limit Sensor Operation .........................................................................................P.82

13-2-2. Limit Sensor Mounting Position Adjustment..........................................................................P.82

13-3. Adjustment of the Position Sensor in the Optical Path Change-Over Turret ............................P.83

13-3-1. Adjustment of the Stop Position Sensor................................................................................P.83

13-3-2. Adjustment of the Zero Position Detection Sensor................................................................P.84

R4820.A

Appendix: Test Mode................................................................................................................................P.85

1. Test Mode Hierarchy ................................................................................................................P.85

2. Displaying Information About the Main Body System...............................................................P.87

2-1. Page 1 -- Checking switch inputs........................................................................................P.88

2-2. Page 2 -- Reading limit sensors..........................................................................................P.89

2-3. Page 3 -- Checking set signals ...........................................................................................P.89

2-4. Page 4 -- Checking the piezoelectric focus drive ................................................................P.90

2-5. Page 5 -- PFS nosepiece information .................................................................................P.90

2-6. Page 6 -- Z motor drive and optical-path change motor drive .............................................P.91

2-7. Page 7 -- Bottom and offset lens motor drives ....................................................................P.91

3. Displaying Information About the Hub System .........................................................................P.92

3-1. Page 1 -- Motorized accessories.........................................................................................P.95

3-2. Page 2 -- Micrometer ..........................................................................................................P.95

3-3. Page 3 -- Stage...................................................................................................................P.96

3-4. Page 4 -- Shutter.................................................................................................................P.96

3-5. Page 5 -- DIA illumination ...................................................................................................P.97

3-6. Page 6 -- Intensilight ...........................................................................................................P.97

3-7. Page 7 -- DSC ....................................................................................................................P.98

3-8. Page 8 -- Handle and knob data .........................................................................................P.98

3-9. Page 9 -- PFS nosepiece PID.............................................................................................P.99

3-10. Page 10 -- Hub-side nosepiece PID....................................................................................P.99

3-11. Page 11 -- Ergonomic controller and joystick information .................................................P.100

4. Displaying/Editing PFS Parameters .......................................................................................P.101

4-1. Page 1 -- Speed control parameters .................................................................................P.102

4-2. Page 2 -- Control speed gain ............................................................................................P.102

4-3. Page 3 -- Automatic adjustment division boundary line.....................................................P.103

4-4. Page 4 -- Offset zero-position setting................................................................................P.104

5. Displaying/Editing Software DIP Switches .............................................................................P.105

5-1. Page 1 -- Speed control parameters .................................................................................P.106

6. Displaying/Editing PFS Parameters (for supporting sold products) ........................................P.107

6-1. Page 1 ..............................................................................................................................P.108

Overview R-4820.A

- 1 -

1. This repair manual covers Ti-E model. 2. This repair manual has been prepared as a textbook for the repair training session. It provides step-by-step instructions that are designed to enable service personnel to gain an

understanding of the structure of the mechanical and optical systems, and to learn how to repair them.

3. When you conduct repair work, be very careful of any sharp edges, projecting object, and

conductive parts to avoid injury or electric shock. (It is recommended that you unplug the power cable and wear safety gloves while you are conducting repair work.)

Points on Repair Use a Phillips screwdriver that appropriately fits the slot in a screw head used. Clean plastic parts with mild detergent. Ether, thinner, alcohol and other organic solvents may cause discoloration or deterioration. To clean the glass parts, first blow the dust off them and then wipe them using lens tissue

moistened with alcohol (or alcohol-ether mixture).

Overview

1. Tools R-4820.A

- 2 -

Tool No Name Illustration J21084 Hex-key set Set (x 6) T91400 Phillips screwdriver set Set (x 4) T91000 Slotted screwdriver set Set (x 6) T91204 Phillips screwdriver

w/wood handle 0.236 in. in diameter

T91102 Slotted screwdriver w/wood handle

0.217 in. width

T91305 Phillips screwdriver bit (For JCIS screw M2, M2.6)

2.5mm115mm

T91320 Phillips screwdriver bit holder 8mm

90mm

T92041 Tweezers AA

T92162 Compass spanner A

T92163 Compass spanner B

E719 Handlap (Alcohol container)

Alcohol (or alcohol-ether mixture), lens tissue, blower, gasoline (for cleaning), bamboo stick (for applying lubricant and adhesive), dial gauge, scraper Lubricant and Adhesive: Refer to Parts Lists (KR-7959).

1-1. Tools for General Use and Preparations

3.543 in.

4.528 in.

0.315 in.

0.098 in.

1. Tools R-4820.A

- 3 -

J25417 Tool legs (four in a set; common to TE2000)

Note: To order, fill in the quantity as four, not as one set.

J25578 Ti Optical path adjustment mount 2 New J25433 Reference revolver for round dovetail (common to TE2000) J25425 Reference straight tube (for objective) (f200)

1-2. Tools for Special Purposes

1. Tools R-4820.A

- 4 -

J25575 Ti Reference straight tube (for body tube) (f210) New J25438 Alignment clamp screw (common to TE2000) Set (x 2) Note: To order, fill in the quantity as two. J25572B Ti Output port straight tube B (V-T straight tube) New J25572A Ti Output port straight tube A New


1. Tools R-4820.A

- 5 -

J25573A,B Ti Reference reticle J25574 Ti Tool body tube base J25220 Crosshair eyepiece J25221 Tool eyepiece


J15573A J15573B

1. Tools R-4820.A

- 6 -

J29102 Dioptric telescope J28005 1-minute reading collimator J28171 Tool for adjusting infinite optical path difference J28003 Parallel mirror

1-2. Tools for Special Purposes 1-2. Tools for Special Purposes

1. Tools R-4820.A

- 7 -

J28004 Grid sample J25194 Dark crosshair sample J25172 Centering scope


0.913 in.

2. Specifications R-4820.A

- 8 -

1. Name Ti-E INVERTED MICROSCOPE 2. Observation Optical System Three-time reflection, Erecting observation, Field number: 22 3. Output Optical System Side port: CCD camera, BA filter wheel, Confocal head, Adapter straight tube for the

photometric sensor (Attachable and removable) Observation port: Used with Ti-T-BPH EYEPIECE TUBE BASE UNIT WITH PH or Ti-T-BS

EYEPIECE TUBE BASE UNIT WITH SIDE PORT 4. Optical Path Selection Light Intensity Distribution EYE: Observation 100%, L100: Left side 100%, R100: Right side 100% L80: Observation 20% Left side 80% Note: R100 and L80 each are replaceable with an option prism. Motor: Two-phase pulse motor Change-over time: 1 sec or less (between adjacent positions) 5. Up-Down Movement Unit Stroke: From the reference position, up: 6.57mm or higher, down: 2.03mm or higher 100.2mm overall Motor: Five-phase pulse motor Operation Speed: Three types (Ex Fine/Fine/Coarse) Maximum speed: 2.5mm/sec (Reference value) Minimum step: 0.025 0.025m Refocus system: Using Escape/Refocus switch Escape is located 2mm below the reference position. 6. Others As requested by a person in charge, other specifications will be added with the approval of Nikon.

2. Specifications

3. Schematic Diagram of the Optical System R-4820.A

- 9 -

3-1. Schematic Diagram of the Ti-E Optical System

3. Schematic Diagram of the Optical System

Optical path change-over prism

Second objective lens

Fig. 3-1

3. Schematic Diagram of the Optical System R-4820.A

- 10 -

3-2. Components in the Optical System

Left-side port optical axis

Right-side port optical axis

Optical path change-over prism

Second objective lens

Objective mounting position

Specimen focus plane

Primary image plane

Optical axis of observation system

3. Schematic Diagram of the Optical System

Fig. 3-2

4. Connection Diagram R-4820.A

- 11 -

4-1. Inner Connection Diagram of Ti-E

4. Connection Diagram

5. Removing/Installing the Optical Unit R-4820.A

- 12 -

Remove the accessories that make up the microscope to prevent them from obstructing the disassembling, assembling and optical system adjustments of the internal components of the microscope. 5-1. Removing the Accessories 1. Stage

Set the movable part of the stage to the center of the movable range in the X and Y directions each and remove the stage.

2. Revolver Remove the revolver with the objective lens attached.

3. Light post

The center of gravity is located in the upper portion of the light post. Remove it being careful it does not to fall over.

4. Body tube Loosen the body tube clamp screw and remove the body tube upward from the body tube adapter.

5. Body tube adapter

Remove the body tube adapter from the microscope base.

5-1. Removing the Accessories

Revolver securing bolts

Stage securing bolts

Post securing bolt

Body tube clamp screw

Adapter securing bolts


- 13 -

5-2. Removing the Optical Unit 1. Remove the button bolts (#0202x2, #0222x2) from

the rear panel. (Fig. 5-1) 2. Lay the microscope base with its rear side down

and remove the hexagonal button bolts (#0202x8) to detach the bottom plate. (Fig. 5-2)

3. Remove the flat cable (#0814).

Step: Disconnect the stopper from J5 connector and the same from the cable retainer on the relay board (#0804). (Fig. 5-3)

5-2. Removing the Optical Unit

Fig. 5-3

Fig. 5-2

Fig. 5-1


- 14 -

4. Remove the hexagonal bolts with washers

(#0108x4) and detach guide plate (#0105). (Fig. 5-4)

5. Disconnect four connectors (J3, J4, J6, and J8)

from the CPU/memory board (#0801). Disconnect two connectors (J1 and J6) from the

interface board (#0802). (Fig. 5-5) 6. Remove the four hexagonal bolts (#203x4) securing

the interface board (#0802) and pull out the board. (Fig. 5-6)

Bolts securing the board

Fig. 5-6

J8

J3

J6

J4

J1

J6

Fig. 5-5

Hexagonal bolts

Fig. 5-4

5-2. Removing the Optical Unit 5-2. Removing the Optical Unit 5-2. Removing the Optical Unit


- 15 -

7. Disconnect the connector (Fig. 5-7) from the encoder cable (#0824) and disconnect the connector

(Fig. 5-8) from the laser safety switch cable (#0684). 8. Loosen the setscrews (#0653x2) securing the

second objective lens selector joint. Move the loosened joint connection in the direction

indicated by the arrow. (Fig. 5-9) 9. Remove the optical unit from the microscope base.

(#0203x6) (Fig. 5-10) [Notes]

When removing the bolts, securely hold the optical unit with your hand to prevent it from falling.

When removing the optical unit, exercise extreme care not to let the unit components bang against the surroundings.

Fig. 5-10

Fig. 5-8Fig. 5-7


Fig. 5-9

Setscrew #0653


- 16 -

10. Attach legs (J25417) to the optical unit. (Fig. 5-11)

Store the optical unit to protect it from dirt and dust. Now the removal of the optical unit is complete.

Fig. 5-11



- 17 -

5-3. Installing the Optical Unit 1. Lay the microscope base with its rear side down. 2. Set the secondary objective lens to the 1x position. 3. Detach the four legs from the optical unit. (Refer to Fig. 5-11.) 4. Put the optical unit into the microscope base while securely holding the unit with your hand. [Notes]

Do not get two harnesses (one connected to the precise encoder and the other connected to the interlock) inside the microscope front caught between the optical unit and the microscope base.

When installing the optical unit, exercise extreme care not to let the unit components bang against any other components.

5. Secure the optical unit while pushing it against the reference mounting plane (left and rear: Refer to Fig. 5-12.) of the microscope base. (#0203x6)

6. Hold the joint against and secure it with two setscrews (#0653x2). (Refer to Fig. 5-9.)

Make sure that you can hear a "click" sound when you change the second objective lens position from 1x to 1.5x.

7. Connect the two harness connectors to each of the precision encoder and interlock inside the microscope base. (Refer to Figs. 5-7 and 5-8.)

8. Secure the interface board with the four hexagonal bolts (#023x4). (Refer to Fig. 5-6.) 9. Connect the four connectors to the CPU and memory board and connect the two connectors to the

interface board. (Refer to Fig. 5-5.) 10. Secure the guide plate (#0105) with the hexagonal bolts with washers (#0108x4). (Refer to

Fig. 5-4.) 11. Attach the flat cable (#0814). (Refer to Fig. 5-3.) Secure the flat cable using the stopper of the cable retainer. 12. Mount the bottom plate with the hexagonal button bolts (#0202x8). (Refer to Fig. 5-2.) 13. Bring the microscope back to horizontal.

5-3. Installing the Optical Unit

Fig. 5-12

Reference planes

6. Disassembling/Assembling the Up-Down Movement Unit R-4820.A

- 18 -

Note: Hereinafter, some illustrations are copied from MEA53100 repair parts list

KR-7959. 6-1. Disassembly

6-1. Disassembling the Up-Down Movement Unit


- 19 -

Some screws are gummed up. Melt the adhesive using alcohol before loosening them. 1. Remove the optical unit. (Refer to 5-2. Removing the Optical Unit.) 2. Disconnect the connector and harness. (Fig. 6-1) 3. Bring the microscope base back to horizontal. 4. Remove the button head screws (#0202x2)

securing the protective cover (#0118) and detach it. Then, slide the sledge (#0130) and remove it.

(Fig. 6-2)


Harness Connector

Fig. 6-1

Button head screw

Fig. 6-2


Fig. 6-2A


- 20 -

5. Remove the up-down movement cover (#0418).

(Fig. 6-3) (Hexagonal button bolts #0457x4)

6. Lift the up-down movement unit in the direction of the arrow to detach it from the microscope base. (Fig. 6-4)

(Hexagonal bolts with washers #0204x4) 7. Detach upper/lower limit sensor board (#0705).

(Fig. 6-5) (Hexagonal bolts #0453x2)

Fig. 6-4

Hexagonal bolts #0453

Limit sensor board #0705

Fig. 6-5


Fig. 6-3

Fig. 6-3A

Fig. 6-5A



- 21 -

8. Loosen the setscrew (#0462x1) and pull out the

linear encoder mirror mounting frame (#0409) to the right in the picture. (Fig. 6-6)

9. Detach the index of the linear encoder from the encoder support frame (#0416). (Fig. 6-8)

(Hexagonal bolts #0451x2) Note: Handle the index with care to prevent disconnection or deformation of a leaf spring.

Mirror mounting frame#0409

Fig. 6-6

Fig. 6-8Fig. 6-7


Setscrew #0462

Fig. 6-6A

Fig. 6-7A


Bolt securing the index #0451


- 22 -

10. Loosen the bolts through the openings of #0416 and pull the glass scale of the linear encoder out

from #0417 to the left in the picture (Fig. 6-9). (Figs. 6-9, 6-9A and 6-10) (Hexagonal bolts #0452x2)

When pulling the glass scale out, exercise care so as not to hit it against surrounding parts.

Fig. 6-10Fig. 6-9

Fig. 6-9A


Bolt securing the scale #0452


- 23 -

11. Detach the encoder preamplifier board from #0416.

(Fig. 6-11) (Hexagonal bolts #0455x3)

12. Remove the up-down movement stroke limiting

screw through #0416. (Fig. 6-12) (Hexagonal bolt with washer #0453x1)

13. Loosen the roller race adjusting screws (#0463x3)

on #0416. (Fig. 6-13)

Fig. 6-11

Fig. 6-12

Fig. 6-13

Fig. 6-11A



- 24 -

14. Aim the protruding part of the encoder downward

and remove the captive screw for the cylindrical roller bearing (#0454) on the opposite side (Fig. 6-15) to the rack.

(One location only) (Fig. 6-14) 15. Remove the hexagonal bolts with washers

(#0456x3) securing the roller race (#0407). (Fig. 6-15)

16. Slide the roller race (#0407) and remove it. (Fig. 6-16)

Fig. 6-14

Fig. 6-15

Fig. 6-16



- 25 -

17. Remove the race support (#0417) at the center from

the up-down movement base. (Fig. 6-17) When you remove the race pad, cylindrical roller

bearing (#0402) comes out and the rollers get scattered. Exercise care so as not to lose them.

Notes Remove the rack (#0406) if necessary. Replace the cylindrical roller bearing (#0402) and retainer (#0411) if rust, dent or wear is found. Remove rust, if any exists, from the roller race mounting surface with vinyl adhesive and fine

sharpening stone. Now, the up-down movement block has been disassembled. Clean the components.

Fig. 6-17


Fig. 6-17A


- 26 -

6-2. Assembly 1. Place the retainer on the roller race secured to the

up-down movement base and alternately put the fourteen cylindrical rollers with lubricant (L4010) applied. (Fig. 6-18)

2. Place the retainer on the roller race secured to the

race support and alternately put the fourteen cylindrical rollers with lubricant (L4010) applied. (Fig. 6-19)

3. Fit the race support into the up-down movement base. 4. Slide the roller race to fit to the unit and secure the race temporarily with the hexagonal bolts

(#0456x3). 5. Attach the captive screw for the cylindrical roller bearing (#0454, #0464) you removed in Step 6-1-14

to the roller race and apply adhesive (#757) to the screw. 6. When you place the retainer on the roller race, adjust the position of the retainer so that the center

of its movable part will align with that of the movable part of the up-down movement unit. 7. Move the race support of the movable parts to the center, tighten the adjusting screws (#0463x3)

(Fig. 6-13) and push the roller race you temporarily secured in Step 6-2-4 against the movable race support.

8. When you move the race support, the cylindrical roller bearing and retainer should smoothly move. Make sure that the race support of the movable parts smoothly moves and no uneven or rough movements exist.

Using a spring scale, push the movable part and ensure that the initial resistance when it starts moving is in the range from 100 to 150 g. If the initial resistance is outside the above range, follow Step 6-2-7 to adjust it using the adjusting screw.

Then, tighten the bolts (#0456x3) you temporarily secured in Step 6-2-4. * Tightening the bolts (#0456x3) may adversely affect the movement of the race support of the

movable parts. If that happens, repeat Step 6-2-7. 9. Apply adhesive (#757) to the threads of the adjusting screws (#0463x3) you tightened in Step 6-2-7.

6-2. Assembling the Up-Down Movement Unit

Fig. 6-18

Fig. 6-19


- 27 -

10. Fit the up-down movement stroke limiting screw (hexagonal bolt with washer #0453x1). 11. Attach the encoder preamplifier board you removed in Step 6-1-11. 12. Attach the scale you removed in Step 6-1-10 to the encoder. 13. Attach the index you removed in Step 6-1-9 to the encoder. 14. Attach the mirror mounting frame you removed in Step 6-1-8. 15. Adjust the encoder following the procedure described in Step 13-1. Encoder Adjustment. 16. Attach the upper/lower limit sensor board you removed in Step 6-1-7. 17. Fit the up-down movement unit into the microscope base, push it against the reference planes

(Fig. 6-20) in the back of the body and on the left side in the direction of the arrow (Fig. 6-21). (Hexagonal bolts with washers #0204x4)

Fig. 6-20 Fig. 6-21


Reference planes

Fig. 4-20A

Reference planes


- 28 -

18. Adjust the position where the limit sensor board is installed following the procedure described in

Step 13-2. Upper- and Lower-Limit Sensor Adjustment. 19. Attach the up-down movement cover (#0418). (Hexagonal button bolts #0457x4) Now, the up-down movement unit has been assembled.


7. Disassembling/Assembling the Joint Gears R-4820.A

- 29 -

7-1. Disassembling the Joint Gears


- 30 -

7-1. Disassembly Some screws are gummed up. Melt the adhesive using alcohol before loosening them. 1. Remove the optical unit. (Refer to 5-2. Removing the Optical Unit.) 2. Bring the microscope base back to horizontal. 3. Remove the protective cover (#0118). Slide the

dummy sledge (#0130) in the direction indicated by the arrow and remove it.

(Hexagonal button bolts #0202x2) (Fig. 7-1)

4. Remove the cover (#0115) from the microscope

base (#0101). (Hexagonal button bolts #0202x2) (Fig. 7-2) 5. Remove the up-down movement unit from the

microscope base. (Refer to 5-2. Removing the Optical Unit.) 6. Lay the microscope base with its rear side down. 7. Disconnect the pulse motor connector. (Fig. 7-3)


Fig. 7-1

Bolts securing the cover #0202

Fig. 7-2

Connector of pulse motor

Fig. 7-3

Fig. 7-1A


- 31 -

8. Detach the joint while holding it from inside. (Hexagonal bolts with washers #0202x4)

Fig. 7-4

Inside the base

Joint

Fig. 7-5


Fig. 7-5A


- 32 -

9. Loosen the two setscrews (#0377x2) and remove

the large reduction gear. (Fig. 7-6)

10. Detach the pulse motor (#0828). (Screws #0373x4) (Fig. 7-7) 11. Loosen the setscrew (#0377x1) and detach the pulley (#0356). (Figs. 7-8 and 7-9) Replace the pulse motor if necessary.


Fixing screws #0373

Fig. 7-7

Setscrew #0377

Fig. 7-8 Fig. 7-9

Fig. 7-6Fig. 7-6A

Reduction gear #0355

Setscrews #0377


- 33 -

Do the following if the reduction gear is required to be disassembled. 12. Remove the pan head screws (x6) and detach the

bearing (#360). (Fig. 7-10) 13. Remove five ball bearings (#0353x5) on the bearing

(#0360). 14. Remove gears (#0351, #0352, #0354, #357, #0358)

and ball bearings (#0353x5) from the shaft support. (Fig. 7-11)

Fig. 7-11


Fig. 7-7, 8, 9A

Fixing screws

Fig. 7-10

Bearing


- 34 -

Now, the joint gears have been disassembled. Check the ten ball bearings for movement. If there is inconsistent movement or galling, replace them.

Fig. 7-10, 11A



- 35 -

7-2. Assembly 1. Fit the ball bearings (#0353x5) into the shaft support. 2. Apply lubricant (G9110) to the teeth of each gear and fit the gears into the ball bearing in the shaft

support. (Refer to Fig. 7-11.) 3. Reinstall the ball bearings (#0353x5) to each gear shaft. 4. Reinstall the bearing and fit the ball bearing into the hole in the bearing (#0360). 5. Secure the bearing with the screws with washer (#0375x6). (Position the bearing using the pin

#0378.) 6. Apply adhesive (#757) to the specified screws. 7. Attach the pulley (#0356) to the pulse motor (#0828). Secure the screws securing the pulley on the D-Cut surface of the shaft of the pulse motor. 8. Reinstall the pulse motor to the joint with the four screws. 9. Reinstall the reduction gear (#0355). 10. Put the joint into the microscope base.

Secure the joint using the hexagonal bolts with washer (#0202x4) in the top of the microscope base while pressing the joint against the reference plane (toward upper left in the picture). (Fig. 7-12)

11. Secure the up-down movement unit with the hexagonal bolts while pressing it against the reference

plane of the microscope base. (Refer to 6-1. Disassembling the Up-Down Movement Unit.) 12. Attach the optical unit while pressing it against the reference plane. (Refer to 5-3. Installing the

Optical Unit.) 13. Bring the microscope base back to horizontal and secure the protective cover with the two

hexagonal button bolts. 14. Attach the cover. 15. Attach the dummy sledge. Now, the joint has been assembled.

Fig. 7-12

7-2. Assembling the Joint Gears

Reference planes

Fig. 7-13

8. Disassembling/Assembling the Coaxial Coarse/Fine Focus Knob R-4820.A

- 36 -

8-1. Disassembling the Coaxial Coarse/Fine Focus Knob


- 37 -

8-1. Disassembling the Coaxial Coarse/Fine Focus Knob 1. Remove the optical unit. (Refer to 5-2. Removing the Optical Unit.) 2. Using an appropriate tool such as chopsticks,

remove rubber cap (#0313) from each of the left and right fine control handles. (Fig. 8-1)

3. Remove fine control knob (#0302) on the right and

left sides. Remove the screws (two each for right and left

side) that secure the fine control knobs on both sides. (Fig. 8-2)

Fig. 8-1

Fig. 8-2

Setscrews


Fig. 8-1A

Fig. 8-2A


- 38 -

4. Remove left decorative ring (#0309) on the left side.

(Fig. 8-3) 1) Loosen the setscrews (#0377x3) that lock

retaining ring (#0305) to remove the ring. 2) Remove the decorative ring. Hexagonal bolts

(#0371x2)

5. Make the microscope upright with its rear side

down. 6. Remove right decorative ring (#0310) on the right

side. (Figs. 8-4 and 8-5) 1) Remove the inner hexagonal bolts (#0372x2).

Note: Be sure to hold the handle unit inside to prevent it from falling.


Fig. 8-3

Hexagonal bolts

Locking setscrews

Fig. 8-4

Hexagonal bolts

Fig. 8-5

Handle unit

Fig. 8-3A

Fig. 8-4A


- 39 -

7. Slide the handle in the direction of the arrow to

remove it from the microscope base. (Fig. 8-6)

8. Loosen the screws (#0376x2) fixing thrust retaining

ring (#0306) to remove it. (Fig. 8-7)

9. Remove Delrin washers (#0301) and corrugated

washer (#0303). (Fig. 8-8) Note: Be sure to remove the two Delrin washers.


Fig. 8-8

Fig. 8-6

Fig. 8-7

Thrust retaining ring #306

Setscrews #376

Fig. 8-5A

Fig. 8-8A


- 40 -

10. Remove the hexagonal bolts (#0372x2) to detach the encoder cover (#0312). (Figs. 8-9 and 8-10)

Note 1: Pay attention to Delrin washer (#0301) between the encoder cover and encoder scale (#0826).

2: The encoder scale tends to bend, so handle it with care. 11. Remove the encoder head (#0824).

1) Remove the two screws (#0375) fixing the encoder head. (Fig. 8-11)

12. Loosen the screws (#0376x2) fixing the thrust

retaining ring (#0308) and remove it. (Fig. 8-12)

Fig. 8-11

Screws fixing the encoder #0375

Fig. 8-12

Fixing screws #0376



Fig. 8-10

Encoder scale #0826

Fig. 8-9

Cover #0312

Hexagonal bolts #0372


- 41 -

13. Loosen the screws (x2) fixing encoder scale

(#0826) and detach the scale from the fine motion shaft. (Fig. 8-13)


Fig. 8-13

Encoder scale #0826

Screws fixing the scale

Fig. 8-9, 10, 11, 12, 13


- 42 -


retaining ring (#0306) and remove it from the handle shaft (#0304). (Fig. 8-14)


retaining ring (#0308) and remove it from the handle shaft (#0304). (Fig. 8-15)

Replace the encoder scale if it is bent or damaged, if necessary. Replace the fine motion shaft if it is bent or damaged, if necessary. Wipe the oil off of the oil holes after disassembling.

Fig. 8-14


Fixing screws #0376

Fig. 8-14A

Fig. 8-15


Fine motion shaft #0304

Fixing screws #0376



- 43 -

8-2. Assembling the Coaxial Coarse/Fine Focus Knob

1. Fit the thrust retaining ring so as to allow the setscrew to seat in the V groove of the fine motion shaft. (Refer to Fig. 8-15.)

2. After applying oil to the encoder holder (#0311), insert the fine motion shaft and fit the thrust retaining ring. (Refer to Fig. 8-14.)

3. Secure the encoder scale to the fine motion shaft with two fixing screws. (Refer to Fig. 8-13.)

4. Secure the thrust retaining ring to the fine motion shaft with two fixing screws. (Refer to Fig. 8-12.)

5. Install the encoder head with two screws. (Refer to Fig. 8-11.) Check that the encoder head does not come into contact with the scale.

6. Fit the Delrin washers and secure the encoder cover with the two hexagonal bolts. (Refer to Figs. 8-9 and 8-10.)

7. Insert the Delrin washer, corrugated washer and another Delrin washer in that order and secure the thrust retaining ring with the two screws. (Refer to Fig. 8-8.)

8. Fit the handle to the microscope and secure the right decorative ring with the two hexagonal bolts.

9. Secure the left decorative ring with the four hexagonal bolts.

10. Attach the retaining ring and secure it with the three locking screws.

11. Secure the right and left fine motion handles with screws, two each side.

12. Attach the rubber cap to each of the fine control handle.

8-2. Assembling the Coaxial Coarse/Fine Focus Knob

9. Disassembling/Assembling the Second Objective Lens R-4820.A

- 44 -

9-1. Disassembling the Second Objective Lens


- 45 -

9-1. Disassembling the Second Objective Lens Some screws are gummed up. Melt the adhesive using alcohol before loosening them. 1. Remove the optical unit from the microscope

base. (Refer to 5-2. Removing the Optical Unit.) 2. Loosen the setscrews (#0654x2) in the fulcrum

(center of rotation) of the magnification variable turret (#0502) and pull the unit out of #0518 upward. (Fig. 9-1)

3. Detach 1x lens cell holder (#0503) and 1.5x lens cell holder (#0507) from the magnification variable

turret. (Figs. 9-2 and 9-3) (Hexagonal bolts with washers #0669x2 each)


Securing bolts #0669

1x lens cell 1.5x lens cell

Fig. 9-2 Fig. 9-3

Fig. 9-1

Setscrews #0654x2

Securing bolts #0669

Setscrew #0653


- 46 -

4. Loosen the setscrew (#0653x2) in the 1x lens cell holder. Rotate the lens cell (#0504) counterclockwise and remove it. 5. Rotate the retaining ring (#0506) counterclockwise and detach it from the lens cell. This causes lens (#L001), separation ring (#0505) and lens (#L002) to detach. Particular care must be exercised so as not to damage the lens in the lens cell. 6. Loosen the setscrew (#0653x2) in the 1.5x lens cell holder. Rotate the lens cell (#0508) counterclockwise and remove it. 7. Rotate the retaining ring (#0510) counterclockwise and detach it from the lens cell. This causes lens (#0016), separation ring (#0509) and lens (#L007) to detach. Particular care must be exercised so as not to damage the lens in the lens cell. 8. Rotate the retaining ring (#0511) counterclockwise and detach it from the lens cell. This causes lens (#L008) to detach. Particular care must be exercised so as not to damage the lens in the lens cell. Now, the second object turret unit has been disassembled. Note: Using lens paper, clean the removed lenses. Wash hardware components.


Fig. 9-4 Fig. 9-5

Fig. 9-6 Fig. 9-7

Optical diagram of 1x lens cell

Optical diagram of 1.5x lens cell


- 47 -

9-2. Assembling the Second Objective Lens 1. Mount the lens (#L008) to the 1.5x lens cell (#0508) as

shown in Fig. 9-7, and then screw the retaining ring (#0511) down tight.

Apply adhesive (#757) to the threads of the retaining ring. 2. Mount the lens (#L007) to the same lens cell as shown in

Fig. 9-7, and fit the separation ring (#0509). Mount lens (#0016) on the separation ring as shown in Fig.

9-7 and screw retaining ring (#0510) down tight. Apply adhesive (#757) to the threads of the lens cell. 3. Screw the lens cell (#0504) onto the 1x lens cell holder

(#0503). Note: Screw the lens cell until you are not able to see its

threads any more. Secure the lens cell with the setscrews (#0653x2) to a

degree where you can rotate it slightly with your finger. (This is used to focus the lens by rotating the lens cell after

fitting the lens cell to the microscope.) 4. Mount the lens (#L002) to the lens cell as shown in Fig. 9-5,

and fit the separation ring (#0505). Mount the lens (#L001) on the separation ring in the

direction shown in the figure, and screw the retaining ring (#0506) down tight.

Apply adhesive (#757) to the threads of the retaining ring. 5. Screw the lens cell (#0508) onto the 1.5x lens cell holder

(#0507). Note: Screw the lens cell until you are not able to see its

threads any more. Secure the lens cell with the setscrews (#0653x2) to a

degree where you can rotate it slightly with your finger.

9-2. Assembling the Second Objective Lens

Fig. 9-8

Fig. 9-9


- 48 -

6. Follow the procedure below to temporarily secure the 1.5x lens cell holder to the magnification variable turret (#0502).

(1) Put the lens cell holder on the magnification

variable turret and secure it with two alignment clamp screws (J25438). (Fig. 9-10)

(2) Remove either of the alignment clamp screws and temporarily secure the lens cell holder with

the hexagonal bolt with washer (#0669). (This is to adjust the optical axis by moving the lens cell after fitting the lens cell to the

microscope.) (3) Remove the other alignment clamp screw and temporarily secure the lens cell holder with the

hexagonal bolt with washer (#0669). (This causes the 1.5x lens cell holder to be temporarily secured to the center of the hole.)

7. Position the 1x lens cell holder to the magnification variable turret using alignment clamp screws

(J25438) and then secure the holder with the hexagonal bolts with washers (#0669x2). Note: The 1x lens cell holder is not to be temporarily secured but must be permanently secured. 8. Secure the magnification variable turret to the optical path change-over unit with the setscrews

(#0654x2). Adjustments are to be carried out in the following sections. 11-4. Focusing and Optical Axis Adjustment on the Objective Side 11-4-2. Adjustment of the Optical Axis and Parfocality of the 1x Second Objective Lens 11-4-3. Adjustment of the Optical Axis and Parfocality of the 1.5x Second Objective Lens Make sure that the magnification variable turret smoothly rotates. Now, the second objective lens has been assembled.

9-2. Assembling the Second Objective Lens

Fig. 9-10

10. Disassembling/Assembling the Optical Path Change-Over Block R-4820.A

- 49 -

10-1. Disassembling the Optical Path Change-Over Block


- 50 -

10-1. Disassembling the Optical Path Change-Over Block * Some screws are gummed up. Melt the adhesive using alcohol before loosening them. 1. Remove the optical unit from the microscope base. (#0664)

a) Remove the optical unit. (Refer to 5-2.) b) Attach four legs (J25417) to the removed optical unit. (Fig. 5-11) c) Loosen the setscrews (#0654x2) on the second objective turret unit and remove the unit. (Refer to 9-1 Disassembling the Second Objective Lens.)

2. Remove the optical path change-over turret. (Fig.

10-1) (Hexagonal bolts on the center of the turret #0665x2) The removed optical path change-over turret must be handled and stored with care so as not to let it bang against the surroundings.

Fig. 10-1


J25417

Fig. 10-2

Fig. 10-3


- 51 -

When the optical path change-over turret is removed, you will be able to see the special washer (#0558). Underneath the special washer, needle bearings (#0557) are arranged in a circle. (Fig. 10-4) Replace the bearing if it does not move smoothly or if it is damaged or deformed.

* When the prism in the optical path change-over unit needs to be replaced, perform the following

procedure. 8. Remove the adhesive from the prism that is to be replaced.

Remove the prism or prism holder fixing screws from the back of the optical path change-over turret and detach the prism. Scrape off any remaining adhesive and clean the prism using alcohol.

9. Apply adhesive (#649) to the bonding surface shown in the illustration given in the part list (Fig. 7 for

MEA53100) and press each prism against the reference plane for bonding. For prism #0006, press it against the optical path change-over turret holder to bond it to the

intended surface. When joining the prism with adhesive, be sure to orientate the prism in the correct direction.

Check the prism in the optical path change-over unit, which is pre-assembled and set up for other similar products.

Now, the optical path change-over turret has been disassembled. Wash and clean the components.

Fig. 10-4



- 52 -

10-2. Assembling the Optical Path Change-Over Block 1. Put the special washer, the needle bearing with lubricant (G9124) applied, and another special

washer in that order into the location shown in Fig. 10-2. 2. Attach the optical path change-over turret. (Hexagonal bolts #0665x2)

Note: The slit in stop position detecting slit disc (#0562) must be at the center of the photointerrupter on stop position detection sensor board (#0818) when prism #0006 is oriented in the optical path. The slit in zero position detecting slit disc (#0565) must then be at the center of the photointerrupter on zero position detection sensor board (#0818).

Refer to 13-3. Adjustment of the Position Sensor in the Optical Path Change-Over Turret. 3. Checks and adjustments are to be carried out in the following sections.

11-1. Preparation for Optical Adjustment 11-2. Adjustment of Stop Position of Turret 11-3. L100 (Prism in Left Observation Optical System) Optical Axis Adjustment

4. Attach the second objective turret unit.

(Refer to 9-2. Assembling the Second Objective Lens.)

10-2. Assembling the Optical Path Change-Over Block

11. Optical Adjustment R-4820.A

- 53 -

Note: Pictures and illustrations in this document are provided for purposes of explaining operation

procedures, so they may differ from what you actually see.

11-1. Preparation for Optical Adjustment Tools to be used Ti Optical path adjustment mount 2 (J25628) 1-minute reading collimator (J28005) Parallel mirror (J28003) Legs (J25417) Preparation 1. Remove the optical unit from the

microscope base. (Refer to 5-2 Removing the Optical Unit.)

2. Attach legs (J25417) to the optical unit. Lift the legs and remove the flat cable support (#0586x3) from the back of the optical unit. (Screw

#0663x6) When using a model that has cable clamps mounted on the back of its base, remove the clamps and clean the surface on which they were mounted. (Remove adhesive residue.)

11-1. Preparation for Optical Adjustment

Fig. 11-1

Fig. 11-2


- 54 -

3. Remove the second objective turret unit from the optical unit. (Refer to 9-1. Disassembling the Second Objective Lens.)

4. Check that R100 (#0007+#0583: prism in the right

observation optical system) and L80/E20 (#003+#0585: prism in the left observation optical system) are pressed against the reference plane of the turret.


Fig. 11-4 Fig. 11-4

Reference pin

Reference plane Reference plane R100

L80/E20

Fig. 11-3

Fig. 11-5


- 55 -

5. Remove the legs from the optical unit and insert the unit into the Ti

Optical path adjustment mount 2 (J25628). (Fig. 11-6) 6. Place the widest section of the optical unit

onto the second post.


Reference plane

Reference plane

Second post

Fig. 11-6

Fig. 11-7


- 56 -

7. Hold a different position on the unit with one hand. Insert the unit again to pass over the rear

reference plane. Pull the optical unit toward the rear reference plane, press it against the side reference plane and then push a lever down to clamp the unit. (Figs. 11-8 and 11-9)

8. Rotate the optical path change-over turret to orient Eye100

(#0006) on the turret in the optical path. (Fig. 11-8) Put the 1-minute reading collimator on the optical path of the Ti Optical path adjustment mount 2 (over the open hole above Eye100).

9. Place the parallel mirror on the top surface of

Eye100 so that it can sit well balanced. (Fig. 11-9) Note: When placing the mirror, exercise care so

as not to cause scratch on the contact surface of Eye100.

10. Measure the inclination of X and Y of the parallel mirror (Eye100).

The reading must be within 6' If the reading is more than 6',please contact to Nikon.

Fig. 11-9


E100

1-minute reading collimator

Fig. 11-8


- 57 -

11-2. Adjustment of Stop Position of Turret 1. Rotate the optical path change-over turret to orient R100 in the optical path.

(Refer to Fig. 11-10.)

2. Put the 1-minute reading collimator on the hole in the top surface of the adjusting base plate. (Refer to Step 4 in 11-1. Preparation: refer to Fig. 11-8.)

Note: Do not move or rotate the position of the 1-minute reading collimator until the adjustment

procedures provided in this chapter are completed.

3. Measure the coordinates of R100 (prism in the right observation optical system). If the reading is more than 6', repeat Step 7 to adjust the mounting position of the click unit (#0547) so that the reading will be within 6'. Adjust the position to bring the reading as close to 0 as possible. Make a note of the value.

4. Rotate the optical path change-over turret to orient L80/E20 (prism in the left observation optical

system) in the optical path. (Fig. 11-11) 5. Measure the coordinates of L80/E20.

The reading must be within 6'. Also, difference in inclination from R100 must be within 3'. Be sure to satisfy these two requirements by adjusting the mounting position of the click unit (#0547).

6. Orient R100 and measure the coordinates.

The reading must be still within 6'.

Fig. 11-10 Fig. 11-11

11-2. Adjustment of Stop Position of Turret


- 58 -

7. To adjust the mounting position of the click unit

(#0547), do the following: 1) Loosen the click unit fixing screws (#0670x2).

2) Adjust the position of the click unit (#0547) by moving the whole unit while looking into the collimator so that the readings will be within the specifications. (Fig. 11-12)

3) After the click position is adjusted, repeat Steps 1 through 6 to check that the specifications are satisfied.

11-2. Adjustment of Stop Position of Turret

Fig. 11-12


- 59 -

11-3. L100 (Prism in Left Observation Optical System) Optical Axis

Adjustment 1. Rotate the optical path change-over turret to orient

L100 (#0007+#0581+#0582) in the optical path. (Figs. 11-13 and 11-14)

2. Measure the coordinates of L100.

The reading must be within 6'. Also, the mutual differences from R100 and L80/E20 must be within 6'. Be sure to satisfy these two requirements by adjusting the angular and rotational direction of L100.

To make the adjustments, do the following: 1) Loosen the fixing screws (#0688x3) on #0582 and

adjust the angular. 2) Loosen the fixing screws (#0688x4) on #0581 and

adjust the rotation direction. 3. After L100 is adjusted, measure the coordinates of

R100 and L80/E20. The readings each must be within 6'.

Each of the following differences in the readings must be within 6': Between R100 and L80/E20, between L80/E20 and L100, and between L100 and R100.

If the requirement above is not satisfied, repeat Steps 11-2 and 11-3.

11-3. L100 (Prism in Left Observation Optical System) Optical Axis Adjustment

#0582 fixing screw (A total of 3)

Fig. 11-13


Fig. 11-14


- 60 -

11-4. Focusing and Optical Axis Adjustment on the Objective Side Tools to be used Reference straight tube (for f200 objective) (J25425) Ti Reference reticle (J25573A) Reference revolver for round dovetail (J25433) Crosshair eyepiece (J25220) Dioptric telescope (J29102) Alignment scope (J25172) 11-4-1. Preparation 1. Remove the diaphragm (#0536) from the optical unit. (Fig. 11-16)

2. Attach Ti Reference reticle (J25573A) to the

reference plane of the optical unit with the diaphragm removed. (Figs. 11-15, 11-16, 11-17 and 11-18) Note: The reference reticle must be correctly

oriented.

Fig. 11-16

Fig. 11-15

Fig. 11-17Reference planes

Fig. 11-18

11-4. Focusing and Optical Axis Adjustment on the Objective Side


- 61 -

3. Attach the optical unit to the Ti Optical path adjustment mount 2. Note: This step is not required when you perform the

adjustment in this chapter following Steps in 11-1 through 11-3.

4. Attach the second objective turret to the optical path

change-over unit. (Setscrews x2) Check that the click stop appropriately works.

5. Orient Eye100 in the optical path. 6. Attach the reference revolver for round dovetail (J25433) to the objective lens with the two fixing

screws and attach the reference straight tube (for objective J25575) on it. (Fig. 11-18) Note: Since the focal position changes during focusing, remove the dustproof glass (#AL01) from

the microscope and insert the glass into the Ti Optical path adjustment mount 2. (Figs. 11-20 and 11-21)

Fig. 11-19


Fig. 11-20Fig. 11-21


- 62 -

11-4-2. Adjustment of the Optical Axis and Parfocality of the 1x Second

Objective Lens 1. Fit the crosshair eyepiece (J25220) to the reference

straight tube and focus on the crossline. (Fig. 11-18) 2. Set the magnification change-over lever to 1x. 3. Temporarily focus on the reference reticle.

Steps: Loosen the setscrews #0653. While looking through the eyepiece, rotate the 1x lens cell. (Fig. 11-22)

4. Make the crossline in the eyepiece coincide with the

center of the reference reticle by adjusting the angular and rotational direction of the mirror unit (#0009+#0534+#0535). (Fig. 11-23)

To make the adjustments, do the following:

1) Loosen the fixing screws (#0668x4) on #0535 and adjust the angular.

2) Loosen the fixing screws (#0688x4) on #0534 and adjust the rotation direction.

Specification: Within 0.004 in. 5. Fit the dioptric telescope (J29102) to the eyepiece

lens and bring each crossline into focus. (Fig. 11-24) 6. Rotate the lens cell to bring the three lines in the

dioptric telescope, crosshair eyepiece and reference reticle into focus.

7. When they are in focus, tighten the screws of the

lens cell. Specification: Within 0.024 in. 8. Remove the dioptric telescope and check that the

crossline in the eyepiece coincides with the center of the reference reticle.

If they do not, repeat Step 4 to adjust the angular and rotational direction of the mirror unit until they coincide.

9. When the optical path is changed from "E100" to

"R100" and "L80/E20", check that the amount of misalignment at the center of the reticle on the respective positions is within 0.006 in. (within 1.5 graduations on the graduated scale on the eyepiece).


Fig. 11-22

Fig. 11-23



Fig. 11-24


- 63 -

11-4-3. Adjustment of the Optical Axis and Parfocality of the 1.5x Second

Objective Lens 1. Change the magnification change-over lever to 1.5x. 2. Temporarily focus on the reference reticle.

Steps: Loosen the setscrews (#0653x2). While looking through the eyepiece, rotate the 1.5x lens cell. (Fig. 11-25)

Note: If the reference scale line is not visible in the view of eyepiece, loosen the bolts (#0669x2: Fig. 9-3) securing the 1.5x lens cell holder (#0507) and move the 1.5x lens cell until the line becomes visible.

3. Loosen the securing bolts on the lens cell holder. (#0669x2) If you loosened them in Step 2, leave them as they are and proceed to the next step. 4. Adjust the position of the lens cell to make the crossline in the eyepiece coincide with the center of

the reference reticle. (Fig. 11-25) When they coincide, secure the lens cell with the hexagonal bolts (#0669x2). Specification: Within 0.004 in. 5. Fit the dioptric telescope to the eyepiece lens and bring each crossline into focus. 6. Rotate the lens cell to bring the three lines in the crosshair eyepiece, dioptric telescope and

reference reticle into focus. 7. When they are in focus, tighten the setscrews. Specification: Within 0.024 in. 8. Remove the dioptric telescope and check that the crossline in the eyepiece coincides with the center

of the reference scale. If they do not, rotate the lens cell until they coincide. 9. When you change the magnification change-over lever from 1.0x to 1.5x or 1.5x to 1.0x, check that

the amount of misalignment from the optical axis and focus satisfies the following specifications: Optical axis: Within 0.06 in. Focus: Within 0.028 in. Now, the objective side has been adjusted. 10. Attach the optical unit to the microscope base. (Refer to 5-3. Installing the Optical Unit.)


Fig. 11-25


- 64 -

11-5-1. Setting Carry out the preparatory steps described in this section before starting the overall adjustment. The main purpose of this section is the same as that of the previous section (11-4. Focusing and Optical Axis Adjustment on the Objective Side). If you have carried out the previous section, you can skip this section. 1. Lay the microscope base with its rear side down. 2. Attach the optical unit while pressing it against the reference positions (on the left and back sides) of

the microscope base. (Hexagonal bolts #0203x6) (Refer to 5-3. Installing the Optical Unit.) 3. Remove four out of the six vibration dampening rubber legs shown

in the figure and replace them with four tool legs (J25417). After replacement, bring the microscope back to horizontal. (Fig. 11-26) Tools to be used Crosshair eyepiece (J25220) Dioptric telescope (J29102) Reference revolver for round dovetail (J25433) Reference straight tube (for objective) (J25425) Ti Reference reticle (J25573A) Ti Reference straight tube for body tube (J2575) Preparation (for focusing the reference reticle and adjustment of optical axis) 1. Attach the optical unit to the inside of the microscope while pressing it against the reference plane of

the microscope base. (Refer to 5-3. Installing the Optical Unit.)

2. Remove the diaphragm (#0536) from the optical unit.

3. Attach the Ti Reference reticle (J25573A) to the optical unit while pressing it against the reference plane. Note: The Ti Reference reticle must be correctly oriented.

4. Bring the microscope back to horizontal.

5. Remove the protective cover and dummy sledge. (Refer to 6-1. Disassembling the Up-Down Movement Unit.)

6. Remove the fixing screw (#0122x4) so that you can easily remove the dustproof glass holder when making adjustments. Note: Take care not to lose the rubber sheet under the dustproof glass holder.

11-5. Preparations Common to Overall Optical Adjustments

Fig. 11-26


- 65 -

7. Loosen the fixing screws (#0216x2) to detach the straight tube from the left-side port mount (if

attached). (This is to allow you to insert your finger into the hole when focusing the second objective lens and

make adjustment of its optical axis.)

8. Attach the reference revolver for round dovetail (J25433) to the microscope. (Hexagonal bolts x2) 9. Turn the optical path change-over dial to E100. 11-5-2. Temporary Adjustment of Focus and Optical Axis Adjustment 1. 1x objective lens

1) Turn the magnification change-over handle to "1x". 2) Fit the crosshair eyepiece (J25220) to the reference straight tube for objective (J25425) with the

dustproof glass holder attached. Turn the diopter ring to bring the crossline into focus. 3) Adjust the focus of the reference reticle by rotating the second objective lens cell (moving the

lens position up or down) on the 1x side. To make the adjustment, do one of the following: a) Insert your finger into the hole in the left-side port and rotate the second objective lens cell on

the 1x side. b) Remove the dustproof glass holder and rotate the second objective lens cell on the 1x side.

4) Follow the procedures below to make the crosshair eyepiece coincide with the center of the

reference reticle. a) Loosen the hexagonal bolts with washers (#0668x4) that secure the adjustment prism to the

main plate to a degree that the prism can be moved by hand (horizontal adjustment). b) Loosen the hexagonal bolts with washers (#0668x4) that secure the adjustment prism to its

support plate to a degree that the prism can be moved by hand (vertical adjustment). c) Manually adjust the position of the prism vertically and horizontally.

5) The amount of misalignment between the crosshair eyepiece and the center of the reference reticle must be within 0.004 in. (within 1 graduation on the graduated scale on the eyepiece).

After confirmation, tighten the hexagonal bolts with washers (#0668x8) that mount the adjustment prism.


Fig. 11-27


- 66 -

6) When the optical path change-over dial is changed from "E100" to "R100" and "L80/E20", check that the degree of misalignment at the center of the reticle on the respective positions satisfies the relevant specifications.

Specification: Within 0.006 in. (within 1.5 graduations on the graduated scale on the eyepiece)

2. 1.5x objective lens (The following is compliant with x1.)

7) Turn the magnification change-over handle to "1.5x". 8) Using the same procedure in Step 3), adjust the focus of the reference reticle by rotating the

second objective lens cell (moving the lens position up or down) on the 1.5x side. 9) Adjust the focus by moving the position of the 1.5x second objective lens cell holder that is

temporarily secured to the magnification variable turret to make the crosshair eyepiece and the center of the reference reticle coincide.

Adjustment procedure:

Insert your finger into the hole in the left-side port and move the second objective cell on the 1.5x side. Alternatively, remove the dustproof glass holder and adjust the focus by moving the position of the second objective lens cell on the 1.5x side. After adjustment, tighten the hexagonal bolts with washers (#0669x2) that mount the second objective lens cell.

Now, the temporary adjustment of the focus of the reference reticle and the optical axis adjustment have been completed. Then, carry out focusing. 11-5-3. Focusing 1. Fit the dioptric telescope to the crosshair eyepiece. Be sure to attach the dustproof glass holder. (Notes) Set the indicator of the dioptric telescope to "0". Turn the diopter ring to bring the double crosshair reticle into focus. Turn the correction ring to bring the scale of the crosshair eyepiece into focus. 2. Turn the optical path change-over dial to "E100". 3. Turn the magnification change-over handle to "1x" and "1.5x" and check that the focus of the

reference reticle that is observed with the graduated scale on the eyepiece diopter satisfies the following specification.

If the specification is not satisfied, as with the temporary adjustment, make fine adjustment by rotating the second objective lens cell (moving the lens position up or down) on the 1x and 1.5x sides.

Specification: Within 0.024 in. (within six graduations on the graduated scale on the eyepiece)

When the magnification change-over handle is changed to a range between 1x and 1.5x: Within 0.028 in. (within seven graduations on the graduated scale on the eyepiece)

4. Upon completion of adjustment, remove the dustproof glass holder from the microscope. Insert the machined hex key through the opening and secure the second objective lens cells on the

1x and 1.5x sides with the setscrews (#0669x2 for each).



- 67 -

5. Tighten the setscrews and then reattach the dustproof glass holder to the microscope. (Put the holder back in place.)

Then, reconfirm the focus of the reference reticle with the magnification change-over handle turned to "1x" and "1.5x" positions.

Now, the focusing has been completed. Then, reconfirm the optical axis. Checking Optical Axis 6. Check that the optical axis satisfies the following specification: Specification: Within 0.004 in. (within one graduation on the graduated scale on the eyepiece)

If the specification is not satisfied, repeat Steps in "11-5-2. Temporary Adjustment of Focus and Optical Axis Adjustment" to make the adjustment again.

7. Turn the magnification change-over handle to "1.5x" and check that the optical axis satisfies the

following specification: Specification: Within 0.004 in. (within one graduation on the graduated scale on the eyepiece)

If the specification is not satisfied, repeat Steps in "11-5-2. Temporary Adjustment of Focus and Optical Axis Adjustment" to make the adjustment again.

8. When the optical path change-over dial is changed from "E100" to "R100" and "L80/E20", check that

the degree of misalignment at the center of the reticle on the respective positions satisfies the relevant specifications.

Specification: Within 0.006 in. (within 1.5 graduations on the graduated scale on the eyepiece) 9. Works to be Performed Upon Completion of Adjustment

1) Secure the dustproof glass holder. (Fixing screws x4) 2) Note: Be sure to insert the rubber sheet between the dustproof glass holder and the microscope

base. 3) After reconfirmation, apply adhesive (#0757) to the threads of setscrews (#0669x2) that are

secured to the second objective lens cells on the 1x and 1.5x sides. Now, the focusing and optical axis adjustment on the objective side using the reference reticle has been completed. Then, move on to the focusing and optical axis adjustment on the observation system.


12. Overall Optical Adjustment R-4820.A

- 68 -

12-1. Focusing and Optical Axis Adjustment on the Observation System

Note: In this chapter, you use electrically-driven functions for some steps. Before starting this chapter, check that an electrical system is OK, or complete "13. Electrical Adjustments".

Tools to be used Crosshair eyepiece (J25220) Dioptric telescope (J29102) Alignment scope (J25172) Light post (product) Ti Reference reticle (J25573A)

Revolver (product) 10x objective lens (product) Ti Reference straight tube for f210 body

tube (J25575) Ti Tool body tube base (J25574)

12-1-1. Preparation 1. Attach the Ti Reference reticle (J25573A) to the

optical unit (Refer to 11-4. Focusing and Optical Axis Adjustment on the Objective Side).

2. Remove the four rubber legs and replace them

with four tool legs (J25417). (Refer to 11-5-1 Setting in the Preparations

Common to Overall Optical Adjustments section.) 3. Bring the microscope back to horizontal. 4. Install the light post (transmitted lighting). (Refer to 5-1 Removing the Accessories.) 5. Attach the Ti Tool body tube base (J25574) to the

microscope. 6. Loosen the screw (#0217) securing the lens cell

(#0112). 7. Loosen the hexagonal bolts in the lens cell holder

(#0111) to a degree it can be moved by hand. (Hexagonal bolts #0207x3)

8. Attach the Ti Reference straight tube for f210 body

tube (J25575) to the Ti Tool body tube base. 9. Fit the crosshair eyepiece lens (J25220) to the Ti

Reference straight tube (for body tube) and turn the diopter ring to bring the crossline into focus.

10. Turn the power on, turn the optical path

change-over dial to "E100" and then turn the power off.


Fig. 12-1


- 69 -

12-1-2. Focusing and Optical Axis Adjustment 1. Rotate the lens cell to bring the reference reticle

into focus. Insert your finger into a space in the tool to rotate

the lens cell and adjust the focus by moving the lens position up or down.

(This should be carried out as a temporary adjustment.)

2. Make the crossline in the eyepiece coincide with

the center of the reference reticle. Make this adjustment by rotating the lens cell by

hand. After adjustment, secure the lens cell holder.

(Hexagonal bolts #0207x3) 3. Fit the dioptric telescope to the crosshair eyepiece. 4. Bring the scale of each of the reference reticle,

crosshair eyepiece and dioptric telescope into focus. The adjustment method is the same as in Step 1. Specification: Within 0.012 in.

Checking Optical Axis Remove the dioptric telescope and check that the crossline in the eyepiece coincides with the center of the reference scale. If they do not, repeat Steps from 2. Specification: Within 0.008 in.

Eccentricity with respect to the reference reticle must be within 0.004 in. Now, the focusing and optical axis adjustment on the observation system has been completed. Then, move on to the beam adjustment.


Fig. 12-3


- 70 -

12-1-3. Beam Adjustment 1. Attach the revolver and mount the 10x objective lens. 2. Fit alignment scope (J25172) to the reference straight tube (tube). 3. Measure the beam and check that it satisfies the following specification. Specification: Within 5' If it fails to satisfy the specification, perform Step 4 described below to make the adjustment. 4. Adjust the beam using the three alignment screws in lens cell holder (#0538).

Note 1. These three alignment screws are not spaced 120 degrees apart with respect to the center

of the lens, so when you turn one of them, the lens may not move in your desired direction. Therefore, you should not adjust the beam using one or two screws. Be sure to use the

three screws to appropriately adjust the beam. (Fig. 12-4) 2. The beam adjustment may result in a slight misalignment of the optical axis. Be sure to

reconfirm the optical axis whenever you have made beam adjustment. For details on rechecking and readjusting

for the optical axis, refer to 12-1-2. Focusing and Optical Axis Adjustment.

Now, the focusing and optical axis adjustment on the observation system has been completed. Then, move on to the focusing and optical axis adjustment on the side port.


Fig. 12-4


- 71 -

12-2. Focusing and Optical Axis Adjustment on the Side Port Tools to be used Light post (product) Stage (product) Crosshair eyepiece (J25220) Dioptric telescope (J29102) Revolver (product) Grip sample (J28004) Dark crosshair sample (J25194)

10x objective lens (product) 1x objective lens (product) Ti Reference straight tube for body tube

(J25575) Ti Tool body tube base (J25574) Ti Output port straight tube A or B J25572 A/B Tool for adjusting infinite optical axis difference

(J28171) 12-2-1. Preparation (for focusing and optical axis adjustment on the right- and

left-side ports) 1. Install the light post (transmitted lighting). 2. Attach the revolver and mount the 10x objective lens. 3. Attach the stage. 4. Fit the dark crosshair sample to the stage. Its deposited surface must face the objective side. 5. Attach the Ti Tool body tube base (J25574). 6. Attach the Ti Reference straight tube for f210 body

tube (J25575) to the Ti Tool body tube base. 7. Fit the crosshair eyepiece to the Ti Reference

straight tube for body tube (J25575). (Fig. 12-5) 8. Turn the diopter ring to bring the crossline into

focus.

12-2. Focusing and Optical Axis Adjustment on the Side Port

Fig. 12-5


- 72 -

12-2-2. Optical Axis Adjustment Note: When you use tool for adjusting infinite optical axis difference (J28171) instead of the 10x

objective lens, Steps 2 and 3 are not necessary. 1. Turn the power on and turn the optical path change-over dial to "E100". 2. Using the focusing handle, bring the sample into focus. 3. By moving the stage, make the crosslines

of eyepiece lens coincide with the center of the sample.

4. Attach the Ti Output port straight tube A (J25572A)

to the left-side mount. (Fixing screws x2) If the straight tube is attached to the left-side

mount, attach the Ti Output port straight tube B (J255572B) and secure it. (Fixing screws x2)

5. Fit the crosshair eyepiece to the Ti Output port straight tube A or B. (Fig. 12-6) 6. Turn the optical path change-over dial to "L100". 7. Loosen the fixing screws (#0209x4) securing the side mount and adjust its position to make the

center of the sample coincide with the center of the crosshair eyepiece. After adjustment, tighten the screws. Now, adjust the optical axis of the right-side port. 8. Replace the Ti Output port straight tube A or B with the right-side mount. (Fixing screws x2) 9. Turn the optical path change-over dial to "R100". 10. Following Steps 5 through 7, adjust the optical axis of the right-side port. 11. After adjustment, turn the optical path change-over dial to "E100".


Fig. 12-6


- 73 -

12-2-3. Focusing Adjustment 1. Replace the 10x objective lens with the 1x lens. 2. Fit the dark crosshair sample to the stage. Its deposited surface must face the objective side. 3. Fit the crosshair eyepiece and dioptric telescope to the observation system. 4. Using the focusing handle, bring the dark crosshair sample into focus. (In this step, bring it into

focus roughly.) 5. Set the diopter ring on the crosshair eyepiece and the indicator of the dioptric telescope to "0". 6. Turn the diopter ring on the dioptric telescope to bring the double crosshair reticle into focus. From now on, keep your hand off the diopter rings on the crosshair eyepiece and on the dioptric

telescope. 7. Using the focusing handle, bring the dark crosshair sample into focus. 8. Press the "Z-RESET" switch on the Ti-E front panel to set the Z axis display to "0". 7. Remove the crosshair eyepiece with the

dioptric telescope attached to it from the reference straight tube (for body tube) of the observation system and attach it to Ti Output port straight tube A or B on the right-side port.

8. Using the focusing handle, bring the dark

crosshair sample into focus.

9. Read the Z axis display. (Measure an amount of misalignment with

respect to the focal position of the observation system.)

Specification: Within 0.008 in.

If the specification is satisfied, proceed to Step 12. If not, proceed to Step 10. 10. Detach the side mount (#0139). (#0209x4) 11. Do one of the following to adjust the focal position: (Fig. 12-7)

Remove the existing adjustment washer. Insert an appropriate adjustment washer between the side mount and microscope base (#0101).

Tips If the amount of misalignment is a negative value, insert an adjustment washer between the side

mount and the microscope base. If the amount of misalignment is a positive value, remove the adjustment washer between the

side mount and the microscope base. The following four types of adjustment washers are provided for focusing: 0.039 in. (#0151A), 0.2 in. (#0151B), 0.012 in. (#0151C), 0.039 in. (#0151D)

Note: Be sure to insert a pair of adjustment washers of the same thickness.


Fig. 12-7


- 74 -

Now, adjust the focus of the left-side port. 12. Remove the crosshair eyepiece with the dioptric telescope attached to it from the Ti Output port

straight tube A or B of the right-side port and attach it to the reference straight tube (for body tube) of the observation system.

13. Following the same procedures in Steps 4 through 8, adjust the focus of the observation system and set the Z axis display to "0".

14. Turn the optical path change-over dial to "L100". 15. Remove the crosshair eyepiece with the dioptric telescope attached to it from the reference straight

tube (for body tube) of the observation system and attach it to Ti Output port straight tube A or B on the left-side port.

16. Following the same procedures in Steps 7 through 11, adjust the focus of the left-side port. 17. Following the procedures given in 12-2-2, check optical axis on the right- and left-side ports and, if

necessary, readjust it.

18. When it is found that the specification is not satisfied when rechecking, readjust the focus following the procedures in Steps 3 through 16.

Now, the focusing and optical axis adjustment on the right- and left-side ports has been completed.


13. Electrical Adjustments R-4820.A

- 75 -

Tools

Oscilloscope 13-1-1. Preparation

1. Remove the up-down movement unit. (Refer to 5-2. Removing the Optical Unit, and 6-1. Disassembling the Up-Down Movement Unit.)

2. Connect J2 connector on preamplifier board (#0702) and J4 connector on interface board (

Documents

Nikon Ti E service manual