Nikon C2 Confocal Stup Manual

CONFOCAL MICROSCOPE C2/C2si

This manual is intended to provide setup instructions for Nikon representatives who have attended the lecture on laser safety and have been trained in setup operations. Attempts by others to set up the product may lead to accidents or equipment failure. NIKON is not responsible for personal injury or equipment damage resulting from equipment setup performed by unqualified persons. Be sure to observe the warnings and cautions described in this manual to ensure safety during setup.

M547E 13.7.Nx.3

WARNING

[Confidential]

i

Introduction

This setup manual is written for service personnel who is installing the Nikon Confocal Microscope C2/C2si.

To ensure correct installation, read this manual carefully before installing this product.

No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.

The contents of this manual are subject to change without notice.

The equipment described in this manual may differ from the actual product in its appearance

This manual contains confidential information. Do not disclose this manual to a third party other than the authorized Nikon personnel.

Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If you note any points that are unclear or incorrect, please contact a Nikon Service Department.

Some of the equipment described in this manual may not be included in the set you are installing.

If you intend to use any other equipment with this product, read the manual for that equipment too.

If this equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Microsoft, Windows, and Internet Explorer are registered trademarks of Microsoft Corporation in the U.S. and other countries. Other product and company names mentioned in this manual are trademarks or registered trademarks of their respective owners.

ii

Contents Introduction ........................................................................................................................................................... i Safety Precautions ............................................................................................................................................... vi

WARNING and CAUTION Symbols ................................................................................................. vi WARNING ................................................................................................................................. vii CAUTION ................................................................................................................................... ix

Chapter 1 System Configuration .................................................................................................................... 1 1.1 System ................................................................................................................................... 1 1.2 System Configuration ............................................................................................................ 2

Chapter 2 Laser Safety .................................................................................................................................... 9 2.1 Laser Paths ............................................................................................................................ 9

2.1.1 Laser Units............................................................................................................... 9 2.1.2 Scan Head ............................................................................................................. 10 2.1.3 Spectral Detector ................................................................................................... 12 2.1.4 Detector Unit .......................................................................................................... 13 2.1.5 Upright Microscope ................................................................................................ 14 2.1.6 Inverted Microscope .............................................................................................. 15

2.2 Safety Labels and Components with Apertures ................................................................... 16 2.2.1 Controller ............................................................................................................... 17 2.2.2 Scan Head and Microscope 80i/90i with DIH ......................................................... 18 2.2.3 Scan Head and Microscope 80i/90i with U-Epi ...................................................... 20 2.2.4 Scan Head and Microscope Ni-E/U [with Trinocular Tube] .................................... 22 2.2.5 Scan Head and Microscope Ni-E/U [with Quadrocular Tilting Tube] ...................... 24 2.2.6 Scan Head and Microscope FN1 [with C-TT-C Trinocular Tube T-C and NI-FL] ... 26 2.2.7 Scan Head and Microscope FN1 [with C1 Y-TT Trinocular Tube and U-Epi] ........ 28 2.2.8 Scan Head and Microscope FN1 [with Quadrocular Tilting Tube] ......................... 30 2.2.9 Scan Head and Microscope AZ100 ....................................................................... 32 2.2.10 Scan Head and Microscope Ti-E/U ........................................................................ 34 2.2.11 C-LU3EX 3 Laser Unit ........................................................................................... 36 2.2.12 LU4A 4 Laser Unit A .............................................................................................. 37 2.2.13 LU-LR 4 Laser PS Rack ........................................................................................ 38 2.2.14 C2-DU3 Detector Unit T ......................................................................................... 39 2.2.15 C2-DUS Spectral Detector Unit ............................................................................. 40 2.2.16 C2-DUT Diascopic Detector Unit ........................................................................... 41

Chapter 3 Part Descriptions .......................................................................................................................... 42 3.1 Scan Head ........................................................................................................................... 42 3.2 Controller ............................................................................................................................. 45 3.3 Laser Unit ............................................................................................................................ 48

3.3.1 C-LU3EX 3 Laser Unit and C1-AOM AOM Unit ..................................................... 48 3.3.2 LU4A 4 Laser Unit A .............................................................................................. 50 3.3.3 LU-LR 4 Laser PS Rack 100V/120V/230V ............................................................ 53

3.4 Detector ............................................................................................................................... 55 3.4.1 C2-DU3 Detector Unit T ......................................................................................... 55 3.4.2 C2-DUT Diascopic Detector Unit ........................................................................... 57

Contents

iii

3.4.3 C2-DUS Spectral Detector Unit ............................................................................. 58 3.5 Microscope .......................................................................................................................... 59

3.5.1 DIH ........................................................................................................................ 61 3.5.2 C-TT-C Trinocular Tube T-C or C1 Y-TT Trinocular Tube ..................................... 61 3.5.3 NI-TT Quadrocular Tilting Tube ............................................................................. 62 3.5.4 NI-TT-E Motorized Quadrocular Tilting Tube ......................................................... 62 3.5.5 NI-AST Analyzer Slider Stopper ............................................................................ 63 3.5.6 AZ-TE100LS Ergonomic Trinocular Tube 100 LS .................................................. 63 3.5.7 Laser Safety Kit for Ti-E/U ..................................................................................... 64

Chapter 4 Preparations .................................................................................................................................. 65 4.1 Checking Items and Tools ................................................................................................... 65

4.1.1 Required Items ...................................................................................................... 65 4.1.2 Tools ...................................................................................................................... 65

4.2 Setup Work Procedure ........................................................................................................ 67 Chapter 5 Setting up Microscopes ............................................................................................................... 69

5.1 Setting up Microscope 80i/90i with DIH ............................................................................... 70 5.2 Setting up Microscope 80i/90i with U-Epi............................................................................. 72 5.3 Setting up Microscope Ni-E/U with Trinocular Tube ............................................................ 73 5.4 Setting up Microscope Ni-E/U with Quadrocular Tilting Tube .............................................. 76 5.5 Setting up Microscope FN1 with Trinocular Tube ................................................................ 79 5.6 Setting up Microscope FN1 with Quadrocular Tilting Tube .................................................. 82 5.7 Setting up Microscope AZ100 .............................................................................................. 85 5.8 Setting up Microscope Ti-E/U .............................................................................................. 86

Chapter 6 Setting up Scan Head ................................................................................................................... 91 6.1 Preparation .......................................................................................................................... 91 6.2 Attaching Ring Adapter ........................................................................................................ 92 6.3 Fixing Scan Head in Place ................................................................................................... 94

6.3.1 Microscope 80i/90i ................................................................................................. 94 6.3.2 Microscope Ni-E/U ................................................................................................. 95 6.3.3 Microscope FN1 ..................................................................................................... 96 6.3.4 Microscope AZ100 ................................................................................................. 97 6.3.5 Microscope Ti-E/U ................................................................................................. 98

6.4 Inserting 1st Dichroic Mirror ................................................................................................. 99 6.5 Connecting Optical Fiber Cables ....................................................................................... 100

Chapter 7 Setting up Diascopic Detector ................................................................................................... 102 7.1 Preparation ........................................................................................................................ 102 7.2 Attaching Optical Path Selector Part .................................................................................. 103

7.2.1 Microscope 80i/90i, Ni-E/U, or FN1 ..................................................................... 104 7.2.2 Microscope AZ100 ............................................................................................... 105 7.2.3 Microscope Ti-E/U ............................................................................................... 109

7.3 Attaching Diascopic PMT ................................................................................................... 111 7.4 Attaching/Connecting Light Guide Fiber ............................................................................ 111

Contents

iv

Chapter 8 Setting up Detector Unit ............................................................................................................. 112 8.1 Preparation ........................................................................................................................ 112 8.2 Inserting Filter Cubes (Optional) ........................................................................................ 113 8.3 Connecting Optical Fiber ................................................................................................... 115 8.4 Attaching Diascopic PMT (MHE47300) ............................................................................. 117

Chapter 9 Setting up Spectral Detector ..................................................................................................... 118 9.1 Preparation ........................................................................................................................ 118 9.2 Releasing Optical Fiber ..................................................................................................... 119 9.3 Installing Spectral Board .................................................................................................... 120 9.4 Connecting Optical Fiber ................................................................................................... 121

Chapter 10 Setting up 4 Laser PS Rack ....................................................................................................... 122 10.1 Preparation ........................................................................................................................ 122

10.1.1 Mountable Laser Power Supplies ........................................................................ 122 10.1.2 Components (Accessories Included) ................................................................... 123

10.2 Mounting Laser Power Supplies ........................................................................................ 125 10.2.1 Power Supplies Mounted ..................................................................................... 125 10.2.2 Positions of Laser Power Supplies ...................................................................... 126 10.2.3 Mounting Procedure ............................................................................................ 129

10.3 Details on Mounting Laser Power Supplies ....................................................................... 131 10.3.1 Mounting Laser Diodes (56RCS Series) .............................................................. 131 10.3.2 Mounting Ar Lasers (IMA Series) ......................................................................... 132 10.3.3 Mounting DPSS Lasers (85BCD/85YCA Series) ................................................. 133 10.3.4 Mounting HeNe Laser (05LGP Series) ................................................................ 134

Chapter 11 Setting up 3 Laser Unit EX ......................................................................................................... 135 11.1 Preparation ........................................................................................................................ 136 11.2 Laser Installation ................................................................................................................ 140 11.3 Laser Rough Adjustment 1 ................................................................................................ 146 11.4 Laser Rough Adjustment 2 ................................................................................................ 156 11.5 Final Adjustment for Transmitting Ar Laser Light through Single-mode Optical Fiber ........ 163 11.6 Final Adjustment for Transmitting HeNe Laser and 405-nm Laser Light through

Single-mode Optical Fiber ................................................................................................. 168 11.7 Sheet Metal Cover Attachment .......................................................................................... 170

Chapter 12 Setting up 4 Laser Unit A ........................................................................................................... 172 12.1 Preparation ........................................................................................................................ 173 12.2 Laser Installation ................................................................................................................ 177 12.3 Laser Rough Adjustment 1 ................................................................................................ 184 12.4 Laser Rough Adjustment 2 ................................................................................................ 194 12.5 Final Adjustment for Transmitting Ar Laser Light through Single-mode Optical Fiber ........ 200 12.6 Final Adjustment for Transmitting Other Laser Light through Single-mode Optical Fiber .. 205 12.7 AOTF Driver Remote Controller Detachment .................................................................... 207 12.8 Operation Check ................................................................................................................ 208 12.9 Sheet Metal Cover Attachment .......................................................................................... 209

Contents

v

Chapter 13 Setting up Controller .................................................................................................................. 210 13.1 Installing Spectral Board (Optional) ................................................................................... 210

Chapter 14 Setting up PC and Software ....................................................................................................... 212 14.1 D/A Board Installation [Only when AOM Unit or 4 Laser Unit A Is Used] ........................... 212 14.2 Network Settings ................................................................................................................ 213 14.3 Software Installation .......................................................................................................... 215

14.3.1 Application Software ............................................................................................ 215 14.3.2 Setup Tool Software ............................................................................................ 215

14.4 Setup ................................................................................................................................. 216 14.4.1 Launching C2SetupTool ...................................................................................... 216 14.4.2 Hardware Configuration Setting ........................................................................... 217 14.4.3 Laser Information Setting ..................................................................................... 218 14.4.4 Scan Setting ........................................................................................................ 222 14.4.5 Confirmation Screen ............................................................................................ 223

14.5 System Adjustment ............................................................................................................ 224 14.5.1 Starting C2CalibrationTool ................................................................................... 224 14.5.2 Checking/Modifying/Creating Device Control Parameter Setting ......................... 225 14.5.3 Checking/Modifying/Creating Laser Calibration Setting ....................................... 226

14.6 System Simple Operation .................................................................................................. 227 14.6.1 [Live] Tab: Image Capturing Tool ......................................................................... 227 14.6.2 [Laser Power] Tab: Controlling Laser Power ....................................................... 228 14.6.3 [Detail Setting] Tab: Checking/Setting Details of XML File .................................. 229

Chapter 15 Cable Connections between Units ............................................................................................ 232 Chapter 16 General Adjustment .................................................................................................................... 237

16.1 Adjustment of Scan Head Inclination ................................................................................. 238 16.1.1 Microscope 80i/90i, Ni-E/U, or FN1 ..................................................................... 238 16.1.2 Microscope AZ100 ............................................................................................... 241 16.1.3 Microscope Ti-E/U ............................................................................................... 242

16.2 Adjustment of Condenser Lens in Front of Pinhole ............................................................ 245 16.3 Compensation of Image Offset for Bidirectional Scanning ................................................. 247 16.4 Microscope Settings for Diascopic DIC Microscopy ........................................................... 250 16.5 Accuracy Verification of Spectral Detector ......................................................................... 252

Chapter 17 Confirmation and Check Sheet Entry .......................................................................................... 255 Chapter 18 Troubleshooting ......................................................................................................................... 260 Chapter 19 Specifications ............................................................................................................................. 262

vi

Safety Precautions

To ensure correct and safe operation, read this manual before using this product.

WARNING and CAUTION Symbols

Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. To ensure correct installation, read this setup manual carefully and thoroughly before installing this product.

Safety instructions in this manual are marked with the following symbols to indicate their importance. For your safety, always follow the instructions marked with these symbols.

Symbol Description

WARNINGDisregarding instructions marked with this symbol may lead to serious injury or death.

CAUTIONDisregarding instructions marked with this symbol may lead to injury or property damage.

Safety Precautions

vii

! WARNING

WARNING

Use of controls or adjustments or performance of procedures other than those specified in this manual may result in hazardous radiation exposure.

1. Laser safety

This laser product is designed and built in compliance with the Performance Standards for Light-Emitting Products established by the U.S. FDA and the IEC Laser Product Safety Standard (IEC 60825-1:2007), except for deviations pursuant to Laser Notice No.50. Users are requested to take all appropriate safety measures specified by the preceding standards, in accordance with local laws and regulations. Use of this laser product must be supervised by a laser safety officer (LSO) and users must follow the LSOs instructions. IEC Class 3B Laser Product Wavelength: 400 to 700 nm (Visible CW (Continuous Wave) laser) Beam divergence: 0.26 to 2.74 rad Maximum power or energy output: Total power of 500 mW Max. (Visible CW laser)

2. Intended product use

This product is used only for microscopic observations. It must be attached to the specified microscope model. Do not use this product for any other purpose.

3. Microscopes and mounting

This product must be mounted to a specified microscope following the mounting procedure below. Failure to do so may result in blindness from inadvertent exposure to laser light emitted from the eyepiece section.

Specified microscope Products required for installation

Upright microscope

80i/90i DIH + C1 TE mount C-TT-C trinocular tube T-C C1 Y-TT trinocular tube

Ni-E/U

C-TT-C trinocular tube T-C NI-TT quadrocular tilting tube C2-NI-TT quadrocular tilting tube adapter NI-TT-E motorized quadrocular tilting tube C2-NI-TT quadrocular tilting tube adapter

FN1

C-TT-C trinocular tube T-C C1 Y-TT trinocular tube NI-TT quadrocular tilting tube C2-NI-TT quadrocular tilting tube adapter

AZ100 AZ-TE100LS ergonomic trinocular tube 100LS AZ 100 stage cover

Inverted microscope

Ti-E/U with laser safety kit Left side port through C1-TI TI mounting adapter

C o m p l i e s w i t h F D A p e r f o r m a n c e s t a n d a r d sf o r l a s e r p r o d u c t s e x c e p t f o rd e v i a t i o n s p u r s u a n t t o L a s e r N o t i c eN o . 5 0 , d a t e d J u n e 2 4 , 2 0 0 7 .

LASER RADIATIONAVOID EXPOSURE TO BEAMCLASS 3B LASER PRODUCTTotal Power 500mW MAX.CW 400~700nmIEC/EN60825-1: 2007

Safety Precautions

viii

WARNING

4. Pre-operation check items Before using the system, always confirm the following:

An objective or a cap is in place on each of the nosepiece sockets.

A dia-illumination lamphouse or an epi-illumination lamphouse is attached.

There are no mirrors or other highly reflective pieces of metal in the area surrounding the lens aperture.

The provided cap is in place on the rear port of the quadrocular tube when the upright microscope Ni-E/U or FN1 is used without the DSC zooming port for quadrocular tube.

A camera or a provided cap is in place on the camera port when the upright microscope FN1 is used with the FN-DP double port.

A cap or a camera is in place on the right side port when the inverted microscope Ti-E/U is used.

5. Never attempt to disassemble This product uses lasers. To ensure safety, never attempt to remove any part of the system. Before beginning operations, confirm that all of the parts listed below are in place. Removal of any part during use may result in unintended exposure of eyes or skin to laser light. Be sure to follow the LSOs instructions when replacing an objective.

Eyepiece tube, nosepiece, objective, epi-fluorescence attachment, stage, lamphouse, scan head, a cap on the rear port of the quadrocular tube for the upright microscope Ni-E/U or FN1 (when the dedicated DSC zooming port for quadrocular tube is not used), a cap or a camera mounted on the FN-DP double port for the upright microscope FN1, and a cap or a camera on the right side port of the inverted microscope Ti-E/U

6. Do not stare the laser light When the laser is being fired, a powerful laser light is emitted from the objective. Do not stare the laser light, and always wear laser-protection goggles when staying within the NOHD (Nominal Ocular Hazard Distance) *1 while the laser is fired. Do not place any reflective objects on the stage or on the optical path of diascopic illumination. Failure to do so results in exposure of eyes or skin to the hazardous laser light emitted from the objective and reflected on those objects.*2

*1: NOHD (Nominal Ocular Hazard Distance): 1.6 m (12.2 m for the AZ100)

*2: Corneal MPE (Maximum Permissible Exposure): 1 W/m2 (when the visible CW laser of 400 to 700 nm is used) Skin MPE (Maximum Permissible Exposure): 2000 W/m2 (when the visible CW laser of 400 to 700 nm is used)

7. Manuals and accessories All manuals and accessories used in this laser product and provided by the distributor must be provided to the user.

Safety Precautions

ix

! CAUTION

CAUTION

1. Setting up the system Setup of this system must be handled by trained service personnel. Assigning this task to any other personnel may result in improper setup, impairing system performance and exposing eyes or skin to the laser beam, resulting in injury.

2. Do not disassemble Disassembling the system may result in electric shock or other hazards. Never attempt to disassemble any piece of equipment included in the system. In particular, disassembling any of the components when the laser is on may cause the laser beam to be improperly emitted from the system. If you discover that the system is not functioning properly, immediately stop use, switch off the power switches to all pieces of equipment, and disconnect the power cables from the outlets.

3. Power supply ratings and power supply cords Before connecting power cords to wall outlets, check your power-line ratings to make sure that the input voltage and current-carrying capacity requirements of equipment are met. Use of a non-conforming power line may result in equipment malfunction, failure, or fire. Always use power cords that satisfy local safety regulations. Always use three-pole grounded outlets. Use of two-pole outlets may result in electric shock.

4. Cable connections Always switch off the power switches to all equipment before connecting or disconnecting cables. To prevent the wires from breaking, do not pull cables by the sheath; instead, firmly grasp the plug when connecting or disconnecting a cable.

5. Countermeasures against problems If you notice that the system is not working properly, quickly abort the software on the PC, turn off all power switches, and disconnect power cords from the wall outlets. If the 4 laser PS rack is abnormal, unplug the two power cables for it.

6. Prevent contact with moisture Water splashed onto any component of the system may cause short circuits, resulting in malfunction. If a piece of equipment is subject to contact with water, immediately switch off the power switch to that piece of equipment and wipe off the water with a dry cloth. If water enters a component, immediately suspend use of the system and disconnect the power cord from the outlet.

7. Do not place any objects on system equipment Never place any objects (heavy ones in particular) on the equipment, except for mounting the laser unit onto the 4 laser PS rack (given in the next caution item 8). Object can injure the human body when dropped. A heavy object may cause the deformation, breakage or malfunction of the equipment.

8. The laser unit and 4 laser PS rack setup The laser unit is designed to be used on top of the 4 laser PS rack. The laser unit is so heavy that it can injure the human body when dropped. To avoid injury, be careful not to accidentally push the laser unit off the 4 laser PS rack.

Safety Precautions

x

CAUTION

9. Handling

1. Handle with care

This system is a precision optical instrument. Make sure that the system is not subject to shock; handle equipment gently. Also be sure to handle cables with care. Optical-fiber cables, in particular, must not be bent or pulled with excessive force to prevent failure, malfunction, or break.

2. Installation conditions

To ensure safe and reliable operations, check the following conditions when installing the system:

To prevent equipment from falling and to avoid eventual failure, install each component of the system on a level surface.

To prevent performance degradation caused by dust or other foreign particles, use the system in a dirt-free environment.

Use the system in the environment specified in Environmental Conditions of Chapter 19, Specifications. To prevent condensation and failure, never attempt to use the system under conditions of high temperature and humidity.

Make sure that the system is not subject to strong vibrations, which may degrade image quality.

To prevent degradation, malfunction, or failure of your laser units and PC, always use power supply that is free from electric noise or sudden voltage fluctuations.

To prevent failure, do not block the air flow of the cooling fan and do not place any obstacles near the cooling fans of the controller, PC, laser units, or 4 laser PS rack. If a particular piece of equipment is installed close to a wall, be sure to provide at least 15 cm of space between the wall and the equipment.

3. Transporting

The laser unit and the 4 laser PS rack must be carried with their bottoms securely held by two or more persons.

Do not push the laser unit when carrying the 4 laser PS rack with laser unit mounted. Doing so may have laser unit fall off the 4 laser PS rack. Be sure to push the 4 laser PS rack when transporting.

The 3 laser unit EX weighs approximately 24 kg. The 4 laser unit A weighs approximately 43 kg. The 4 laser PS rack weighs approximately 20 kg. Take care not to get your hand or finger pinched.

4. Cleaning

Use a soft dry cloth to clean painted, plastic, and printed parts. If necessary, wipe these parts with a cloth moistened with diluted neutral detergent, then wipe off the moisture with a soft dry cloth. To prevent discoloration or deformation of painted or plastic parts or removal of the printed letters/figures, do not use organic solvents such as paint thinner or alcohol.

5. Other precautions

Be sure to follow the instructions specified in this manual and in the manual supplied with the software. Failure to do so may result in impaired system performance, malfunction, failure, or unexpected hazards.

1

1.1 System

Figure 1.1-1

1 System Configuration

1 System Configuration >> 1.2 System Configuration

2

1.2 System Configuration Table 1.2-1: System overview (sheet 1 of 3)

Unit Name Code No. Remarks

Scan head &

controller unit

C2-SH C2 scanner and controller MHA43200 Including the controller and optical fiber

C2-SHS C2si Ready scanner and

controller MHA43300

Including the controller and optical fiber Available to be set up with the spectral detector

1st Dichroic

mirrors

(only the model

number of each

filter is shown)

C1 SI-BS 20/80 MHE46900 Select the mirror suitable for the laser. C1 1STDM 405/488/561 MHE46310

C1 1STDM 408/488/543 MHE46300

C2 1STDM EX405/488/594 MHE46500

C2 1STDM EX440/514/594 MHE46510

C2 1STDM EX 457 (440)/561/640 (633) MHE46520

C2 1STDM EX 457 (440)/543/640 (633) MHE46540

C1 1STDM 405/488/543/640 MHE46410

C1 1STDM 405/488/561/640 MHE46420

C2 1STDM EX440/514/561/640 MHE46530

Detector units C2-DU3 Detector Unit T MHE41400

Fluorescence

filter cube

(only the model

number of each

filter is shown)

C2-F 447/60 MHE46610 Used for 2nd DM See Table 1.2-4: Recommended filters. C2-F 482/35 MHE46620

C2-F 494/41 MHE46630

C2-F 510/84 MHE46640

A1-F 515/30 MHE57060

A1-F 525/50 MHE57030

C2-F 537/26 MHE46650

C2-F DAPI/CY5 Dual MHE46660

C2-F 514/30 585/65 MHE46690 Used for 3rd DM See Table 1.2-4: Recommended filters. C2-F 525/50 561LP MHE46700

C2-F 525/50 594LP MHE46710

C2-F 537/26 561LP MHE46720

C2-F 550/49 594LP MHE46730

C2-F 585/65 635LP MHE46740

C2-F 593/40 635LP MHE46750

C2-F 515/30 595/50 MHE46760

C2-F 525/50 595/40 MHE46770


3

Table 1.2-1: System overview (sheet 2 of 3)


Diascopic

detector &

adapter

C2-DUT diascopic detector unit MHE47300

C1-TD-J transmit adapter J MHE47700 Used for 80i/90i, Ni-E/U, or FN1 Applicable to A1 and C2

C2-DUT-AZ DUT mounting adapter MHE47800 Used for AZ100

A1-DUT-TI DUT mounting adapter MHE50330 Used for Ti-E/U Applicable to A1 and C2

Spectral

detector C2-DUS spectral detector unit MHE43000

Usable only when C2si scan head is mounted

4 laser unit &

accessories

LU4A 4 laser unit A MHF41402

LU4-C2 connection Kit MHF41412 Required when 4 laser unit A is used LU-LR 4 laser PS rack 100V MHF44000

LU-LR 4 laser PS rack 120V MHF44001

LU-LR 4 laser PS rack 230V MHF44002

3 laser unit &

accessories

C-LU3EX 3 laser unit MHF42305

C1-AOM AOM unit MHE48000

C1-AREX EX changer MHF43000

Switching 488/514 nm Required when a multiple lines Ar laser is used

C-LU3EX594 DM set MHV55100 Required only when the 594-nm solid-state laser is set to L3 position

Laser adapter C1-LAR408 laser adapter R MXA22043 Used for 3 laser unit EX C1-LAS408 laser adapter S MXA22044 Used for 3 laser unit EX Laser adapter 405/445/640C MXA22103 Used for 4 laser unit A C-LAR488 laser adapter MXA22070 Applicable to 3 laser unit EX/4 laser unit A C-LAR532/561 laser adapter MXA22069 Used for 3 laser unit EX Laser adapter 561 C MXA22110 Applicable to 3 laser unit EX/4 laser unit A Laser adapter 594 S MXA22109 Applicable to 3 laser unit EX/4 laser unit A


4

Table 1.2-1: System overview (sheet 3 of 3)


Peripheral

devices for

microscopes

C-RAL ring adapter L MXA22031 Applicable to any microscope C-RAS ring adapter S MXA22032 Applicable to any microscope

C2-RA C2 ring adapter MHV40000 Applicable to any microscope (referred as SS in tables)

D-DH-E-A1 digital imaging head for A1 MBD76105 Used for 80i/90i C1 Y-TT trinocular tube *1 MHB42200 Used for 80i/90i + U-Epi or FN1 C-TT-C trinocular tube T-C *1 MHB43200 Used for 80i/90i + U-Epi, Ni-E/U, or FN1 U-EPI FL universal epi attachment D-FL MBE74100 Used for 80i/90i or FN1 C1 TE mount MXA22033 Used for 80i/90i + DIH

A1-DIH interlock cable MXA22099 Used for 80i/90i + DIH Applicable to A1 and C2

NI-TT-E

motorized quadrocular tilting tube *2 MBB94510

Used for Ni-E

NI-TT quadrocular tilting tube *2 MBB94521 Used for Ni-E/U or FN1 NI-RPZ-E motorized

DSC zooming port for quadrocular tube *2 MBB96710

Used for Ni-E

NI-RPZ

DSC zooming port for quadrocular tube *2 MBB96721

Used for Ni-E/U or FN1

NI-FLT6-E

motorized epi-fluorescence cube turret MBE94300

Used for Ni-E/U

NI-FLT6-I

intelligent epi-fluorescence cube turret MBE94400

Used for Ni-E/U

NI-FLT6 epi-fluorescence cube turret MBE94500 Used for Ni-E/U or FN1 NI-FLEI epi-fluorescence attachment for Ni MBE94100 Used for Ni-E/U or FN1 NI-BAW-E motorized barrier filter wheel MBE94620 Used for Ni-E NI-PAU Ni photoactivation unit MBE94800 Used for Ni-E/U C2-NI-TT quadrocular tilting tube adapter MHV40100 Used for Ni-E/U or FN1 + quadrocular tube NI-AST analyzer slider stopper *3 MXA22120 Used for Ni-E/U or FN1

A1-TII interlock cable MXA22094 Used for Ni, FN1 + quadrocular tube, or Ti Applicable to A1 and C2

FN-DP variable magnification double port MBD75100 Used for FN1 AZ-TE100LS

ergonomic trinocular tube 100LS MHB43500

Used for AZ100

AZ 100 stage cover MXA22102 Used for AZ100 AZ-FL epi-fluorescence attachment MNE84000 Used for AZ100 C1-TI TI mounting adapter MHV55050 Used for Ti-E/U

Ti laser safety kit MXA22097 Used for Ti-E/U Applicable to A1 and C2

Software NIS-Elements C MHS50000 Applicable to A1 and C2 *1: Allowable combinations of the upright microscope FN1 and trinocular tube are as follows:

a With the C-TT-C trinocular tube T-C, the NI-FLT6 epi-fluorescence cube turret and the NI-FLEI epi-fluorescence attachment for Ni are applicable.


5

b With the C1 Y-TT trinocular tube, the U-EPI FL universal epi attachment D-FL is applicable (FN-DP variable magnification double port can be added).

*2: Allowable combinations of the quadrocular tilting tube and DSC zooming port for quadrocular tube are as follows:

a With the NI-TT-E motorized quadrocular tilting tube, NI-RPZ DSC zooming port for quadrocular tube or NI-RPZ-E motorized DSC zooming port for quadrocular tube are applicable.

b With the NI-TT quadrocular tilting tube, NI-RPZ DSC zooming port for quadrocular tube only is applicable.

*3: NI-AST analyzer slider stopper is necessary for the combinations shown below:

a When any of the NI-FLT6 epi-fluorescence cube turret, NI-FLT6-E motorized epi-fluorescence cube turret, or NI-FLT6-I intelligent epi-fluorescence cube turret is used with the upright microscope Ni-E/U

b When the NI-FLT6 epi-fluorescence cube turret is used with the upright microscope FN1

Table 1.2-2: Items provided by the distributor

Unit Name Remarks

PC Host PC system The 64-bit version of Windows 7, 1-Gbit Ethernet

LAN cable 1000BASE-T, crossover cable (Cat 5e minimum)

D/A board PCI-6711, PCI-6713, or PCI-6733 (National Instruments made)

Microscopes &

accessories

Microscope

Focus motor Provide the one suitable for the microscope.

Power cables For C2 controller (x 1)

100 VAC area: 125 VAC 7 A or higher;

200 VAC area: 250 VAC 6 A or higher

For 4 laser unit A (x 1) 100 VAC area: 125 VAC 7 A or higher;


For AOM controller (x 1) 100 VAC area: 125 VAC 7 A or higher;


For 4 laser PS rack (x 2)


200 VAC area: 250 VAC 6 A or higher, and



For detail, see Chapter 19, Specifications.


6

Table 1.2-3: Recommended lasers

Type Product SpecificationsMounted to

3 laser unit EX Mounted to

4 laser unit A

CW violet

diode laser

Manufacturer: Melles Griot

Head: 56 RCS/S 2780

Power supply: 56 IMA021

400 to 410 nm

(36 mW)

C1-LAS408 is

required.


Head: 56 RCS/S 2781

Power supply: 56 IMA021

440 to 445 nm

(20 mW)

C1-LAS408 is

required.

Manufacturer: Coherent

Head: CUBE405

Power supply: CUBE series

4055 nm

(50, 100 mW) ---

405/445/640C is

required.


Head: CUBE445


4455 nm

(40 mW) ---

405/445/640C is

required.


Head: RADIUS 405

Power supply: CDRH control box

4055 nm

(25 mW)

C1-LAR408 is

required.

---

CW air-cooled

argon ion laser


Head: IMA 101040 ALS

Power supply: 300 series

457 to 514 nm

(40 mW)

AREX EX changer

is required.


Head: IMA 101065 ALS

Power supply: 300 series

457 to 514 nm

(65 mW)

AREX EX changer

is required.

CW blue

solid-state

laser


Head: 85BCD010, 85BCD020, 85BCD030

Power supply: 85BCD series

4880.5 nm

(10, 20, 30 mW)

C-LAR488 is

required.

C-LAR488 is

required.


Head: Sapphire 488 LP

Power supply: Sapphire LP USB CDRH

Driver

4882 nm

(10, 20, 25, 30,

50, 75, 150,

200 mW)

C-LAR488 is

required.

C-LAR488 is

required.

CW green

helium-neon

laser


Head: 05LGP193

Power supply: 05LPL951-065 for 100V

05LPL915-065 for 115/230V

543.5 nm

(1 mW)

CW yellow

solid-state

laser


Head: 85YCA010, 85YCA020, 85YCA025

Power supply: 85YCA series (accessory)

561 nm

(10, 20, 25 mW)

C-LAR532/561 is

required.


Head: Sapphire 561LP-50

Power supply: Sapphire LP USB CDRH

Driver

5602 nm

(50 mW)

561C is

required.

561C is

required.

Manufacturer: Spectra Physics

Head: Excelsior 594 (CDRH model)

Power supply: Excelsior-PS-XC-CDRH

593.70.5 nm

(30, 50 mW)

594S is

required.

594S is

required.


7

Type Product SpecificationsMounted to

3 laser unit EX Mounted to

4 laser unit A

CW red

helium-neon

laser

Manufacturer: JDS Uniphase

Head: 1125P

Power supply: 1202-3 for 100V,

1202-1 for 115V,

1202-2 for 230V

633 nm

(5 mW) ---

CW red diode

laser


Head: 56RCS/S 2782

Power supply: 56IMA020

640+2/-5 nm

(15 mW)

C1-LAS408 is

required.


Head: CUBE640


640+2/-5 nm

(40 mW) ---

405/445/640C is

required.

Laser diode manufactured by Melles Griot is shown in Nikon specification model (Nikon-dedicated model name).

For the beam divergence and the maximum power, see the manual supplied with each laser.


8

Table 1.2-4: Recommended filters

Excitation laser Filter cube

1st laser 2nd laser 3rd laser 4th laser 2nd DM 3rd DM

408 488 543 --- C2-F 447/60

(MHE46610)

C2-F 514/30 585/65

(MHE46690)

408 488 561 --- C2-F 447/60

(MHE46610)

C2-F 525/50 561LP

(MHE46700)

408 488 594 --- C2-F 447/60

(MHE46610)

C2-F 525/50 594LP

(MHE46710)

440 514 561 --- C2-F 482/35

(MHE46620)

C2-F 537/26 561LP

(MHE46720)

440 514 594 --- C2-F 482/35

(MHE46620)

C2-F 550/49 594LP

(MHE46730)

457 543 640 --- C2-F 494/41

(MHE46630)

C2-F 585/65 635LP

(MHE46740)

457 561 640 --- C2-F 510/84

(MHE46640)

C2-F 593/40 635LP

(MHE46750)

488 543 640 --- A1-F 515/30

(MHE57060)

C2-F 585/65 635LP

(MHE46740)

488 561 640 --- A1-F 525/50

(MHE57030)

C2-F 593/40 635LP

(MHE46750)

514 561 640 --- C2-F 537/26

(MHE46650)

C2-F 593/40 635LP

(MHE46750)

408 488 543 640 C2-F DAPI/CY5 Dual

(MHE46660)

C2-F 515/30 595/50

(MHE46760)

408 488 561 640 C2-F DAPI/CY5 Dual

(MHE46660)

C2-F 525/50 595/40

(MHE46770)

9

2.1 Laser Paths

2.1.1 Laser Units

4 laser unit A 3 laser unit EX

Figure 2.1-1

(1) L1 (See the table below.)




(5) Motorized shutter

(6) Laser coupling mirror

(7) Laser outlet (single-mode optical fiber)

(8) Continuous brightness adjustment part (optional AOM unit)

(9) Wavelength selector

(10) AOTF

Table 2.1-1

L1 L2 L3 L4

3 laser unit EX

405-nm laser 440-nm laser 638-nm laser R-HeNe laser 594-nm solid-state laser

Ar laser (488 nm or 514 nm)

Single line or

multiple lines

488-nm solid-state laser

G-HeNe laser 561-nm solid-state laser

594-nm solid-state laser *

4 laser unit A 638-nm laser 405-nm laser 440-nm laser

Ar laser Single line or

multiple lines


G-HeNe laser 561-nm solid-state laser


* The laser coupling mirror needs to be replaced with the C-LU3EX594 DM set (MHV55100) when the 594-nm solid-state laser is set to L3 position on the 3 laser unit EX.

2 Laser Safety

2 Laser Safety >> 2.1 Laser Paths

10

2.1.2 Scan Head

C2 scan head (C2-SH)

Figure 2.1-2

(1) Laser inlet (single-mode optical fiber)

(2) Collimator lens

(3) 1st dichroic mirror

(4) Scanner mirror

(5) Scanner lens

(6) Condenser lens

(7) Pinhole

(8) Fluorescence outlet (multi-mode optical fiber)


11

C2si scan head (C2-SHS)

Figure 2.1-3

(1) Laser inlet (single-mode optical fiber)

(2) Collimator lens

(3) 1st dichroic mirror

(4) Scanner mirror

(5) Scanner lens

(6) Condenser lens

(7) Pinhole

(8) Fluorescence outlet to the detector unit (multi-mode optical fiber)

(9) Fluorescence outlet to the spectral detector (multi-mode optical fiber)


12

2.1.3 Spectral Detector

[Optical path detail]

Figure 2.1-4

(1) Fluorescence inlet into the spectral detector (multi-mode optical fiber)

(2) Collimator lens

(3) Polarized beam splitter

(4) Total reflection mirror

(5) Fresnel rhomb wavelength plate

(6) Diffraction grating

(7) Concave mirror

(8) Optical detector array

(6) (5) (4) (2) (1)

(8) (7)

(8) (7)

(7)

(2) (3)

(1)

(4)

(5)

(6)


13

2.1.4 Detector Unit

Figure 2.1-5

(1) Multi-mode optical fiber

(2) Condenser lens

(3) Filter cube (2nd DM)

(4) Filter cube (3rd DM)

(5) Collimator lens

(6) Optical detector (channel 1)




14

2.1.5 Upright Microscope

Figure 2.1-6

Scan head

Laser inlet

Single-mode optical fiber

Multi-mode optical fiber

Spectral detector

Detector unit

Light guide fiber

Laser beam

Optical path selector part of the diascopic detector


15

2.1.6 Inverted Microscope

Figure 2.1-7

Optical path selector part of the diascopic detector Light guidefiber

Spectraldetector

Detectorunit

Laser inlet

Single-modeoptical fiber

Multi-mode optical fiber Scan head

Laser beam

2 Laser Safety >> 2.2 Safety Labels and Components with Apertures

16

2.2 Safety Labels and Components with Apertures

(1) IEC warning label Laser hazard symbol

(2) IEC explanatory label (3) Safety standard label



Figure 2.2-1 Figure 2.2-2 Figure 2.2-3

(4) IEC aperture label (5) IEC aperture label (6) IEC caution label Class 3B areas

AVOID EXPOSURELASER LIGHT IS EMITTEDFROM THIS APERTURE

CAUTION CLASS 3BLASER RADIATION WHEN OPENAVOID EXPOSURE TO THE BEAM

VORSICHT LASERSTRAHLUNGKLASSE 3B,WENN ABDECKUNG GEFFNETNICHT DEM STRAHL AUSSETZEN


(7) IEC caution label Class 3B areas


(9) Safety standard label


(10-1) Safety standard label (100V)*

(10-2) Safety standard label (120V, 230V)*

(11) IEC caution label Class 4 areas


* These labels are for the 4 laser PS rack. The safety label is different depending on the ratings of your region.


17

2.2.1 Controller


(2) IEC explanatory label (3) Safety standard label






Figure 2.2-16

(1)

(2)

(3)


18

2.2.2 Scan Head and Microscope 80i/90i with DIH


(4) IEC aperture label (6) IEC caution label Class 3B areas





Figure 2.2-20

Figure 2.2-21

E

A

C

B

(6)

(6) (1)

D

(4)

(7)


19

A. Component with aperture B. Component with aperture C. Component with aperture


D. Component with aperture E. Head

Figure 2.2-25 Figure 2.2-26

(7)

Aperture

(6)

Aperture

(6)

Aperture

(6)

Aperture

(6)


20

2.2.3 Scan Head and Microscope 80i/90i with U-Epi







Figure 2.2-30

Figure 2.2-31

F

A

DC

E

B

(6)

(6)

(1) (4)

(7)


21



D. Component with aperture E. Component with aperture F. Head


(7)

Aperture

(7)

Aperture

(6)

Aperture

(6)

Aperture

(6)

Aperture

(6)


22

2.2.4 Scan Head and Microscope Ni-E/U [with Trinocular Tube]






Figure 2.2-41

F

A

DC

E

B

B

(6)

(4)

(7)

(6)


23





(7)

Aperture

(7)

Aperture Aperture

(6)

Aperture

(6)

Aperture

(6)

(6)


24

2.2.5 Scan Head and Microscope Ni-E/U [with Quadrocular Tilting Tube]






Figure 2.2-51

F

A

D

C

E

B B

(6)

(4)

(7) (6)


25





(7)

Aperture

(7)

Aperture Aperture

(6)

Aperture

(6)

Aperture

(6)

(6)


26

2.2.6 Scan Head and Microscope FN1 [with C-TT-C Trinocular Tube T-C and NI-FL]






Figure 2.2-61

F

A

D

C

E

B

(6)

(4)

(7)

(6)


27





(7)

Aperture

(7)

Aperture Aperture

(6)

Aperture

(6)

Aperture

(6)

(6)


28

2.2.7 Scan Head and Microscope FN1 [with C1 Y-TT Trinocular Tube and U-Epi]







Figure 2.2-71

Figure 2.2-72

F

A

D

C

E

B

B

H

G

(6)

(6)

(1)

(4) (6)

(6)

(7)


29





G. Component with aperture H. Component with aperture


(7)

Aperture

(7)

Aperture

(6)

Aperture

(6)

Aperture

(6)

Aperture

(6)

Aperture

(6)

(6)


30

2.2.8 Scan Head and Microscope FN1 [with Quadrocular Tilting Tube]






Figure 2.2-84

F

A

DC

E

B

(6)

(4)

(7) (6)


31





(7)

Aperture

(7)

Aperture Aperture

(6)

Aperture

(6)

Aperture

(6)

(6)


32

2.2.9 Scan Head and Microscope AZ100


(4) IEC aperture label








Figure 2.2-95

Figure 2.2-96

F

A

DC

B

E

(4)

(1) On each objective

(4)

(6)

(6)

(1)

(6)

(7)

(8)


33





(7)

Aperture

(7)

Aperture

(6)

Aperture

(6)

Aperture

(6)

Aperture

(6)


34

2.2.10 Scan Head and Microscope Ti-E/U


(4) IEC aperture label (5) IEC aperture label

AVOID EXPOSURELASER LIGHT IS EMITTEDFROM THIS APERTURE







*: Affix another pair of these labels on the other side of the microscope.

Figure 2.2-108

C

B A

(6)

(6)

(5) * (6) *

Aperture of analyzer point

(6) (1)

Objective aperture

(6)

Aperture (4)

(7)


35



(7)

Aperture

Aperture

(6)

Aperture

(6)


36

2.2.11 C-LU3EX 3 Laser Unit




Figure 2.2-114

(1)

(8)


37

2.2.12 LU4A 4 Laser Unit A


(9) Safety standard label (11) IEC caution label Class 4 areas


Figure 2.2-118

(1) (11)

(9)

View of Arrow A

A


38

2.2.13 LU-LR 4 Laser PS Rack

(10-1) Safety standard label (100V)

(10-2) Safety standard label (120V, 230V)


Front view

Rear view

Figure 2.2-121

(10-1) or (10-2)


39

2.2.14 C2-DU3 Detector Unit T




Figure 2.2-122

Figure 2.2-123

(6)

(6)


40

2.2.15 C2-DUS Spectral Detector Unit




Figure 2.2-124

View without the top cover

Figure 2.2-125

Internal cover

(6)


41

2.2.16 C2-DUT Diascopic Detector Unit




Figure 2.2-126

Figure 2.2-127

(6)

(6)

42

3.1 Scan Head

The scan head is the main optical unit of the system. It is made up of various components, including the galvano-scanner, pinhole unit, 1st dichroic mirror, and optical system components. The excitation light from the laser unit is led into this unit through a single-mode optical fiber, and the fluorescence from the specimen is led into the detector unit through a multi-mode optical fiber.

Two types of the scan head are available; the C2 scan head (MHA43200) and C2si scan head (MHA43300). The C2si scan head can be combined with the spectral detector, and the transmission of the fluorescence from the specimen is switchable to the detector unit or spectral detector.

WARNING

Do not remove the scan head from the microscope. Removing the scan head may cause the laser beam to be emitted through the scan head mounting hole, resulting in injury.

Front view Side view Rear view

Figure 3.1-1 C2si scan head (1) FC connector for the laser unit

This connector is used to transmit excitation light (the laser beam) from the laser unit into the scan head.

(2) SMA connector for the detector unit

This connector is used to transmit the fluorescence coming through the pinhole to the detector unit.

(3) FC connector for the spectral detector (only on the C2si scan head)

This connector is used to transmit the fluorescence coming through the pinhole to the spectral detector.

3 Part Descriptions

3 Part Descriptions >> 3.1 Scan Head

43

CAUTION

Do not touch the optical fiber while images are being captured, as doing so may deteriorate the images.

Do not detach the optical fiber from the scan head to prevent performance degradation and protect eyes and skin from laser radiation.

Optical-fiber cables, in particular, must not be bent or pulled with excessive force to prevent breakage.

(4) X connector

Connector for X-axis galvano scanner control

(5) Y connector

Connector for Y-axis galvano scanner control

(6) I/O connector

Connector for the motor control

(7) Laser indicator

The indicator lamp lights up in orange when the laser is ready to emit beam through the objective. The laser does not emit beam when the indicator lamp is not lit.

(8) 1st dichroic mirror (optional)

The 1st dichroic mirror, which is replaceable, is to separate the optical paths of excitation light and fluorescence depending on the excitation wavelength. For the types of the 1st dichroic mirror, see Table 1.2-1: System overview in Chapter 1, System Overview.

After 1st dichroic mirror replacement, the condenser lens may need to be adjusted. For details, see the condenser lens adjustment screw (11).

Figure 3.1-2

CAUTION

It is an optical component to separate the optical paths of laser beam and fluorescence. If the glass is not clean, the fluorescence yield lowers, thus do not touch the glass surface. In addition, damage on the metal part may cause performance deterioration; carefully handle it.

Select the 1st dichroic mirror for the laser (wavelength) used. After 1st dichroic mirror replacement, be sure to replace the filter cubes in the detector unit suitable for the wavelength.

To use the spectral detector, use the 1st dichroic mirror BS20/80. Other types of 1st dichroic mirror are also usable, but the spectrums obtained depend on the spectroscopic characteristics of the 1st dichroic mirror used.

3 Part Descriptions >> 3.1 Scan Head

44

(9) 1st dichroic mirror inlet

Insert the 1st dichroic mirror.

CAUTION

Do not allow any substance other than the 1st dichroic mirror to come into the 1st dichroic mirror inlet. Otherwise, a failure may occur. If a reflective substance is put in the inlet, the laser beam may irradiate eyes or skin, and it is dangerous.

(10) Optical path selector lever

Switch the lever to select whether to transmit the fluorescence from the specimen to the detector unit or spectral detector. Inside the optical path selector, a 100%-reflection mirror is installed.

Table 3.1-1

Fluorescence detector Lever direction

Detector unit

(filter)

Standard

(slanting)

Spectral detector

(diffraction grating)

Spectrum

(vertical)

(11) Condenser lens adjustment screw

It is placed at the access area for condenser lens adjustment in front of the pinhole. Although the 1st dichroic mirror is manufactured with high precision, pictures may get darkened after replacement or repairing. For such a case, acquire an image turning this adjustment screw with the hexagonal screwdriver supplied with the microscope and make an adjustment so that the center of the image is the brightest.

If not properly adjusted, fluorescent brightness may fluctuate during capturing images.

When adjusting, conform to the recommended condition shown below.

Specimen: Uniform fluorescence specimen Objective: Magnification of x60 or higher Pinhole size: S1 Field of view: Maximum

Figure 3.1-3

Adjust the condenser lens by turning the screws

3 Part Descriptions >> 3.2 Controller

45

3.2 Controller

The controller is used to control this system and has been adjusted with a set of the scan head.

The controller controls each unit and modulated laser, and generates images. Refer to Chapter 15, Cable Connections between Units for information on wiring.

CAUTION

Always use power cords that satisfy the appropriate ratings. Make sure your power cords meet the local safety requirements.



Figure 3.2-1 C2 controller

(1) Remote power button

Press the button; the power is turned on, the LED lights in blue, and the controller becomes active. To turn the power off, press the button while the power is on.

Do not turn the power off while the NIS-Elements C, the operation software for C2, is running.

(2) READY indicator

This indicator lights in green when the C2 controller activates normally.

(3) SCAN indicator

This indicator lights in orange when the Galvano scanner is in operation.

(4) ERR indicator

This indicator lights in red for 1 second when a failure of the C2 controller occurs.

It also lights in red if the system is in the laser interlock status.

(1)

(2)

(3)

(4)

(7)

(6)

(5)

(8)

(9)(10)

(11)(12)(13)

(15)

(18)

(14)

(19)

(17) (16)

(20)

(21) (22)

(23)

(25)

(26)

(24)


46

(5) LASER indicator

This indicator lights in orange while a laser is coming into the scan head.

This indicator may fail to light when the incident light intensity is not sufficient.

(6) LU indicator

This indicator lights in blue when communication between the 4 laser unit A and the controller is normal.

(7) DUS indicator

This indicator lights in green when communication between the spectral detector and the controller is normal.

(8) Detector connector

This connector is used to control the detector unit.

(9) 3EX connector

This connector is used to control the shutters inside the 3 laser unit EX.

(10) AOM connector

This connector is used to control the AOM controller.

(11) X connector

This connector is used to control the X-axis galvano scanner.

(12) Y connector

This connector is used to control the Y-axis galvano scanner.

(13) I/O connector

This connector is used to control the scan head motor.

(14) ILK connector

This connector is used for interlock signals.

(15) 4LU connector

This connector is used to control the 4 laser unit A.

(16) R connector

This connector is used for Ch1 of the detector unit.

(17) B connector


(18) G connector


(19) W connector

This connector is used for ChT (for diascopic detector) of the detector unit.

(20) Spectral board connector (optional)

This connector is used to control the spectral detector.


47

(21) Ext Trg Out connector

This connector is used to output external trigger signals.

(22) Ext Trg In connector

This connector is used to input external trigger signals.

(23) LAN connector

This connector is used for communication with PC. It is 1-Gbit Ethernet compliant.

(24) AC inlet

(25) Power switch

This is the main power switch of the controller. When this switch is on, power is supplied to the primary source only. Power is not supplied to the secondary source (stand-by state).

(26) Fuse box

3 Part Descriptions >> 3.3 Laser Unit

48

3.3 Laser Unit

3.3.1 C-LU3EX 3 Laser Unit and C1-AOM AOM Unit

CAUTION

The laser unit is designed to be used on top of the 4 laser PS rack. The laser unit is so heavy that it can injure the human body when dropped. To avoid injury, be careful not to accidentally push the laser unit off the 4 laser PS rack.

The 3 laser unit EX can accommodate up to 3 lasers. For the accommodatable laser types, see Table 1.2-3: Recommended lasers in Chapter 1, System Overview. For brightness control, turn the brightness control knob for the laser or install the optional AOM unit and use software. It has motorized shutters by laser, which can be opened/closed from the software. For use of the multi-line Ar laser, the optional AREX EX changer allows selection between 488 nm and 514 nm.

3 laser unit EX AOM controller (optional)

Figure 3.3-1 (1) Laser 1

One laser selected from the 405-nm laser, 440-nm laser, 638-nm laser, R-HeNe laser, or 594-nm solid-state laser can be attached.

(2) Laser 2

One laser selected from the Ar laser or 488-nm solid-state laser can be attached.

(3) Laser 3

One laser selected from the G-HeNe laser, 561-nm solid-state laser, or 594-nm solid-state laser can be attached.

(4) Single-mode optical fiber

(2)

(1)

(3)

(4)

(5)

(6)

(7)

(8) (6)

(6)

(16)

(15)

(14)(13) (12)(11)

(10)

(9)


49

(5) AOM connector

This connector is used for driving the AOM.

(6) Light control knob (optional AOM)

This knob controls light intensity in 11 discrete steps or continuously. Transmittance figures obtained when adjusting an Ar laser is shown in the table below. Laser beam does not emit when the marker on the knob is in the unusable area shown in the figure at right. You can also use the optional AOM unit to control light output.

Figure 3.3-2 Table 3.3-1

Transmittance (%) 100 80 50 25 10 3.5 1.3 0.5 0.15 0.05 0.02

(7) Shutter connector

This connector is used to control the shutters inside this unit.

(8) Ar wavelength selector (optional)

Select one of multi-line Ar laser wavelengths (488 nm or 514 nm).

(9) POWER switch

This is the main power switch of the AOM controller. When this switch is on, power is supplied to the primary source only. Power supply to the secondary source is subject to the state of the REMOTE switch (10).

(10) REMOTE switch

This is the remote power switch of the AOM controller. When this switch is on, power is supplied to the secondary source in conjunction with the power supply to the PC. When this switch is off, power is supplied to the secondary source when the POWER switch (9) is on, independently of the power supply to the PC.

(11) AC inlet

(12) C1-SYNC connector

This connector is used to input Sync signals.

(13) PC connector

This connector is used to control the AOM.

(14) LD-MOD connector (not used)

(15) LD-PWR connector

This connector is used to control power of LD (laser diode).

(16) AOM connector

This connector is used for driving the AOM.

Continuous light control area

Marker Step-by-step lightcontrol area

Unusable area


50

3.3.2 LU4A 4 Laser Unit A

CAUTION

The laser unit is designed to be used on top of the 4 laser PS rack. The laser unit is so heavy that it can injure the human body when dropped. To avoid injury, be careful not to accidentally push the laser unit off the 4 laser PS rack.

The 4 laser unit A can accommodate up to 4 lasers. For the accommodatable laser types, see Table 1.2-3: Recommended lasers in Chapter 1, System Overview. To adjust laser intensity, control the AOTF with software. Each laser on this unit is equipped with a software-controlled motorized shutter. Each one of the four orange LEDs on the front of the unit turns on when the motorized shutter is opened and the corresponding laser is emitted into the scan head. When the motorized shutter is closed, the corresponding LED is turned off.

Figure 3.3-3

Figure 3.3-4


51

(1) Laser 1

The 638-nm laser can be attached.

(2) Laser 2

One laser selected from the 405-nm laser or 440-nm laser can be attached.

(3) Laser 3

One laser selected from the Ar laser or 488-nm solid-state laser can be attached.

(4) Laser 4

One laser selected from the G-HeNe laser, 561-nm solid-state laser, or 594-nm solid-state laser can be attached.

(5) Single-mode optical fiber

(6) POWER indicator

This indicator lights in green when the power is turned on.

(7) L1 indicator

This indicator lights in orange when the shutter for laser 1 is opened.

(8) L2 indicator


(9) L3 indicator


(10) L4 indicator


(11) POWER switch

This is the main power switch of the 4 laser unit A. When this switch is on, power is supplied to the primary source only. Power supply to the secondary source is subject to the state of the REMOTE switch (13).

(12) AC inlet

(13) REMOTE switch

This is the remote power switch of the 4 laser unit A. When this switch is on, power is supplied to the secondary source in conjunction with the power supply to the C2 controller. When this switch is off, power is supplied to the secondary source when the POWER switch (11) is on, independently of the power supply to the C2 controller.

(14) AOTF/PC connector

This connector is used to control the AOTF.

(15) CONTROLLER connector

This connector is used to control the 4 laser unit A.

(16) USB connector

This connector is for future expansion. (Do not use in this system.)


52

(17) CONF connector

This connector is used for interlock signals with the controller.

(18) MIC connector

This connector is used for interlock signals with the microscope.

(19) TIRF connector

This connector is intended for extension. It is connected with the shorting pin jack (MHF41412).

When this connector is open, the laser interlock status is set and the shutters cannot be opened.

(20) LD connector

This connector is for future expansion. (Do not use in this system. Otherwise, a malfunction occurs.)

(21) LUSU connector

This connector is intended for extension. It is connected with the rectangular shorting connector (MHF41412).

When this connector is open, the laser interlock status is set and the shutters cannot be opened.


53

3.3.3 LU-LR 4 Laser PS Rack 100V/120V/230V

The 4 laser PS rack houses the power supplies for lasers mounted on the laser unit, and turns on multiple lasers or emits all lasers at a time. The laser unit can be mounted on top of the 4 laser PS rack.

Front view

Rear view

Figure 3.3-5

(1)

(2)

(3)

(4)

(5)

(6)

(7)(8)

(9)

(10)


54

(1) EMISSION laser indicator

This indicator lights in green when the laser emits.

(2) POWER laser indicator

This indicator lights when the power is turned on. The color varies depending on the system status. Orange: Only the 4 laser PS rack control system is energized. No laser is turned on (stand-by state). Green: The 4 laser PS rack control system and all lasers are energized.

(3) EMISSION key switch

This key switch turns on the laser and emits laser. When the key is in vertical position ( O ): The lasers stop emitting and the key can be extracted. When the key is in horizontal position ( I ): The lasers start emitting.

(4) Power switch

This is the main power switch of the 4 laser PS rack. When turning on this switch only, no laser is turned on (stand-by state).

(5) INTERLOCK connector

This connector is used to disable the remote interlock function. When the connector is open, no laser emits (remote interlock state). To disable the remote interlock function, attach the provided interlock jumper connector.

(6) Cooling fan

This fan cools the power supply devices in the 4 laser PS rack. When lasers are energized, the fan starts.

(7) Power breaker for 15 A line

This breaker protects over-current of the 4 laser PS rack controller and the Ar laser power supply. Normally it is turned on.

(8) Power breaker for 5 A line

This breaker protects over-current of the lasers other than Ar laser. Normally it is turned on.

(9) AC inlet for 15 A line

This inlet supplies power to the 4 laser PS rack controller and the Ar laser. When no power is supplied to the inlet, the 4 laser PS rack does not work.

(10) AC inlet for 5 A line

This inlet supplies power to lasers other than the Ar laser.

CAUTION

Use a power cable that satisfies the power ratings of the AC inlet and the safety standard of the country.

3 Part Descriptions >> 3.4 Detector

55

3.4 Detector

3.4.1 C2-DU3 Detector Unit T

This unit functions as the optical detector for confocal microscopy.

The fluorescence emitted from the specimen is led into this unit through the objective, scan head, and optical fiber for fluorescence (multi-mode optical fiber). Set two filter cubes (optional) in the unit to get desired fluorescence detection from each PMT (photomultiplier).

It is equipped with 3 PMTs, which can serve for simultaneous detection with 3 channels.

Figure 3.4-1

(1) Filter slot cover

Open this cover, and then change the filter cube (optional).

(2)

(3)

(4)

(5) (6)

(1)

(9)

(7)

(8)

(10)

3 Part Descriptions >> 3.4 Detector

56

(2) Diascopic detector mounting part

The diascopic detector (optional) is mounted to this part.

(3) T-PMT connector

This connector is used to control the diascopic detector.

(4) CONT connector

This connector is used to control the detector unit.

(5) PMT connector

This connector is used for Ch1 to Ch3, and ChT signals of the PMT.

(6) Multi-mode optical fiber inlet

(7) Filter cube (for 2nd DM) dovetail groove

Insert the filter cube for 2nd DM along with the dovetail groove.

(8) Filter cube (for 3rd DM) dovetail groove

Insert the filter cube for 3rd DM along with the dovetail groove.

(9) Filter cube adapter

Attach the filter cube (10) to the filter cube adapter, and then insert it along with the dovetail groove (7) or (8).

< How to attach the filter cube to the filter cube adapter >

Insert the filter cube to the filter cube adapter.

Figure 3.4-2

(10) Filter cube (optional)

This filter cube is designed to filter out excitation light and to separate fluorescence. For the types of the filter cube, see Table 1.2-1: System overview in Chapter 1, System Overview.

Also for the combination of the filter cubes, see Table 1.2-4: Recommended filters in Chapter 1, System Overview.

Figure 3.4-3

Filter cub

Documents

Nikon C2 Confocal Stup Manual