Newsletter Department of Biochemistry and Molecular Biology

www.med.monash.edu.au/biochem

NEWS AND EVENTS

December 2016

How our immune system targets tuberculosisEvery 18 seconds someone dies from tuberculosis - it is the world’s most deadly infectious disease.

Mycobacterium tuberculosis, the causative agent of tuberculosis (TB), has infected more than one-third of the human population with an annual death toll of approximately 1.5 million people.

For the first time, an international team of scientists from Monash and Harvard Universities have seen how, at a molecular level, the human immune system recognises TB infected cells and initiates an immune response. Their findings, published in Nature Communications, are the first step toward developing new diagnostic tools and novel immunotherapies.

Lead author Professor Jamie Rossjohn from the Department of Biochemistry and Molecular Biology and Monash Biomedicine Discovery Institute said one of the main reasons for our current lack of knowledge was the complexity of the bacterium itself. Working with Professor Branch Moody’s team at Harvard, they have begun to gain key insight into how the immune system can recognise this bacterium.

Crucial to the success of M. tuberculosis as a pathogen is its highly unusual cell wall that not only serves as a barrier against therapeutic attack, but also modulates the host immune system. Conversely, its cell wall may also be the “Achilles’ heel” of mycobacteria as it is essential for the growth and survival of these organisms. This unique cell wall is comprised of multiple layers that form a rich waxy barrier, and many of these lipid components represent potential targets for T-cell surveillance.

Specifically, using the Australian Synchrotron, the team of scientists have shown how the immune system recognises components of the waxy barrier from the M. tuberculosis cell wall.

“With so many people dying from TB every year, any improvements in diagnosis, therapeutic design and vaccination will have major impacts,” Professor Moody says.

“Our research is focussed on gaining a basic mechanistic understanding of an important biomedical question. And may ultimately provide a platform for designing novel therapeutics for TB and treat this devastating disease,” Professor Rossjohn concludes.

Link to article: https://www.ncbi.nlm.nih.gov/pubmed/27807341

Image coutesy of Google Fungal Xmas tree Top: Talaromyces stipitatus, Tree: Aspergillus nidulans Ornaments: Penicillium marneffei, Trunk: Aspergillus terreus. Courtesy of J. Craig Venter Institute.

CHRISTMAS SHUTDOWN PERIOD UNIVERSITY IS CLOSED FROM 22nd Dec until

2nd Jan 2017 ****

Page 2Department of Biochemistry and Molecular BiologyNewsletter: December, 2016

Mobilising our immune system to fight fluOctober marked the 20th anniversary of Professors Doherty and Zinkernagel’s Nobel Prize in Medicine for discoveries concerning the immune system. 20 years on, and Monash-based researchers are making fundamental advances in how our immune system functions.The key to all immune responses – whether we are fighting off a cold, or our body is trying to eliminate a cancerous cell – are T cells. The T cell is the part of the immune system that recognises a foreign invader, such as the influenza virus, and triggers the cascade of events that kills such devastating pathogens. But the mechanism by which these T cells are recruited to fight disease remained unclear.Now a team of Monash researchers has provided key insight into how T cells ‘see’ viruses. In a landmark paper in the journal Immunity, researchers from the Monash Biomedicine Discovery Institute and the ARC Centre of Excellence in Advanced Molecular Imaging, have investigated this recognition in the arsenal of T cells that sit in wait to respond to virus infection.“It was thought that a common mechanism underpinned how our T cells recognised viruses, like a key in a lock” Professor La Gruta said. “But this is not the case. Our data suggests that T cells can recognize viruses in a completely different way, and still ‘unlock’ a T cell response. This discovery is critical because the way this initial T cell-virus interaction occurs shapes the entire immune response that follows.”Using the National Synchrotron, Dr Stephanie Gras, was able to gain key molecular insight into how the T cell sees the virus, and in doing so, challenged immunological concepts spanning decades.“This discovery is a major turn-around in the way we think of how the immune system works,” Professor La Gruta said.“Its fitting that in the 20th anniversary of Australian, Peter Doherty, being awarded The Nobel Prize in Physiology or Medicine with Rolf M. Zinkernagel for their insights into the way the immune system works, we are still unraveling new ways in which the human body fights off disease.”Read the full article: https://www.ncbi.nlm.nih.gov/pubmed/27717799

Breakthrough on virus infecting rare and endangered parrotsAustralian researchers have unravelled the molecular makeup of a virus threatening some of the world’s most endangered species, paving the way for the potential development of a vaccine to save dwindling populations of the Australian birds.The research, led by Charles Sturt University (CSU) scientists and published in the international journal Nature Communications, revealed the structure of the smallest self-replicating virus behind beak and feather disease (BFD).The virus causes a debilitating disease affecting four rare species of native parrot, including the Western ground and Orange-bellied Parrots, of which less than 50 remain in the wild.Fellow researcher on the study, Associate Professor Fasséli Coulibaly, from the Department of Biochemistry and Molecular Biology and Monash Biomedicine Discovery Institute, said: “Our research revealed how the virus particles are pieced together and how the genome is packaged. To build this molecular model, we combined advanced structural biology approaches available at the Australian Synchrotron and the Ramaciotti Centre for Cryo Electron Microscopy.”CSU Professor in Veterinary Pathobiology Shane Raidal said, “The finding is significant because, by confirming how the viral structure forms, we can begin to develop a vaccine to interrupt these processes.”The BFD virus programs only two proteins to drive its replication and spread: one to assist the reproduction of the viral DNA, and one to construct the outer shell of the virus. This shell is built from 60 individual capsid proteins that self-assemble and fit together in a highly specific and ordered arrangement around the viral DNA.The outcomes of this research provide the atomic coordinates of approximately 200,000 atoms which make up the virus, and insights into how the viral DNA can bind to the shell, ensuring the protection and delivery of the viral DNA.At the Australian Synchrotron in Melbourne, landmark research infrastructure of the Australian Nuclear Science and Technology Organisation (ANSTO), the Micro Crystallography (MX2) beamline produced X-rays more than a million times brighter than the sun to create intricate diffraction patterns as light bounced off microscopic crystals of the viral capsid proteins. This allowed the researchers to identify the locations of the individual atoms and broader structure of the viral shells in stunning 3D detail.The research team, involving scientists from CSU, Monash University, the Australian Synchrotron, and Spain’s National Microbiology Centre and the Autonomous University of Madrid, have been working on the project since 2009.Along with the loss of habitat and feral predators, the BFD virus is one of the main threats to the affected parrots, which also include the Norfolk Parakeet (Norfolk Island) and the Swift Parrot (eastern and southern states). Infected birds face starvation and death as their feathers moult and their beaks soften.Mr Barry Baker, Chair of the national Orange-bellied Parrot Recovery Team said it’s important new and innovative fields of science work across conservation projects to protect Australia’s at-risk fauna.“BFDV is an awful disease, especially in small, short-lived species like Orange-bellied Parrots – young birds with the virus stand little chance of survival in the wild, and affected captive birds are often compromised, as the virus can affect the bird’s ability to fight off other health issues.”

Read the full article: https://www.ncbi.nlm.nih.gov/pubmed/27698405

A/Prof Fasséli Coulibaly

Page 3Department of Biochemistry and Molecular BiologyNewsletter: December, 2016

Victoria’s status as biomedical research hub strengthened with major prize win for MonashTwo of Victoria’s leading research minds from Monash University and the University of Melbourne have received the state’s most prestigious scientific award, the Victoria Prize for Science and Innovation, for world-leading research into how the immune system identifies and fights disease. This recognises the collaboration of two pioneering researchers who have worked collaboratively over 15 years of technical innovation.

NHMRC Australia Fellow, Monash University Professor Jamie Rossjohn, and University of Melbourne Deputy Vice-Chancellor (Research), Professor James McCluskey, received the Victoria Prize for Science and Innovation at a ceremony in Melbourne, celebrating a long partnership that has, amongst many finds, led to a pioneering understanding of how T lymphocytes from the immune system recognise harmful microbes. Their research could assist the treatment of inflammatory bowel disease, peptic ulcers, tuberculosis and lead to better vaccines and diagnostic tools.

Presenting the prize, Victorian Minister for Small Business, Innovation and Trade, the Hon. Philip Dalidakis MP, said the honour was further evidence of Victoria’s claim as Australia’s premier biomedical research hub.

“Innovation in science and medical research allows us to develop new skills, create new products and access new markets that will not only enhance the lives of many Victorians, but will drive our economy into the future,” Minister Dalidakis said.

“Congratulations to Professor Jamie Rossjohn and Professor James McCluskey for receiving Victoria’s highest scientific honour. It’s fantastic to see our state’s leading researchers being recognised for the work they do – they are the reason we are a world-leading medical research hub,” Minister Dalidakis concluded.

Not only is Victoria home to a world-class critical mass of creativity and brainpower, but the state has an unparalleled suite of cutting-edge research institutes and facilities, technology platforms and data centres that creates an unparalleled medical research ecosystem.

Professor Rossjohn said the research partnership mirrored the Victorian biomedical research sector’s technological evolution, with the State Government’s decades of vision and commitment paying dividends that will continue to be realised into the future.

“When I completed my PhD in 1994, the computing power was a fraction of that of the smartphone now in my pocket. It took essentially the entire duration of a PhD to determine a protein structure, but now, fortunately, with the advancement of technologies, research progress in this vital area of biomedical science can be much more rapid,” Professor Rossjohn said.

“The investment in the Australian Synchrotron by the Victorian Government has really propelled our research,” Professor Rossjohn added. “Prior to 2008, we used to collect data in overseas synchrotrons. The facilities overseas were great, but very limiting in terms of access: it was very hard to be internationally competitive until the Synchrotron came along,” he explained.

“To have such a world-class facility on our doorstep has been of enormous value to our research. It means we can compete with the best in the world in a sustained manner.”

Professors Rossjohn and McCluskey noted that their success aligned with significant investments in infrastructure from Monash and Melbourne Universities along with the State of Victoria.

“The University of Melbourne has invested in state of the art flow cytometry facilities and other platform technologies in the Doherty Institute and Bio21 Institute,” Professor McCluskey said. “And, Monash has invested in sophisticated imaging platforms, including crystallisation robotics forming part of the Monash Biomedicine Discovery Institute.”

“Across our state the combination of clinicians, medical researchers, cutting-edge laboratories, biomedical companies and landmark research infrastructure provides an amazing and seamless network of moving parts dedicated to finding new and better ways of treating the diseases that affect so many Victorians,” Professor McCluskey said.

Prof Jamie Rossjohn and Mr Philip Dalidakis MP

For more Biochemistry news and events:please visit our website

www.med.monash.edu.au/biochem

Environmental Sustainability At MonashAnyone concerned with any environmental issues should contact

Shani Keleher (shani.keleher@monash.edu) or visit The Office of Environmental Sustainability (TOES)

http://www.fsd.monash.edu.au/environmental-sustainability.

Page 4Department of Biochemistry and Molecular BiologyNewsletter: December, 2016

Nanoparticle breast cancer treatment buoyed by fellowshipLaser-activated nanoparticles that destroy breast cancer cells sound like the fodder of science fiction – but this cutting-edge technology is exactly what Dr Kylie Wagstaff from the Monash Biomedicine Discovery Institute researches. To support this research, Dr Wagstaff has just been awarded a Career Development Fellowship from the National Breast Cancer Foundation.Dr Wagstaff, from the Department of Biochemistry and Molecular Biology, said her research would help to better target breast cancer treatments, as well as aiding the diagnosis of tumours.“The project aims to develop a new series of laser-activated nanoparticles that will specifically deliver and release drugs to breast cancer cells only, without harming nearby healthy cells. This should result in much better outcomes for patients,” Dr Wagstaff said.“The particles can also be seen using medical imaging techniques such as CT and MRI, but only when they accumulate (such as at a cancer site), so these particles can be used simultaneously for diagnostic and therapeutic purposes.”Dr Wagstaff has been awarded almost $670,000 over four years to fund her research.“I am honoured to receive this fellowship, and also very excited as this work will allow me to establish my own research group, as well as extend my interdisciplinary collaborations,” she said.The Career Development Fellowship scheme aims to develop the most outstanding and creative breast cancer researchers across a range of research disciplines.

Dr Kylie Wagstaff

Immunity researcher recognised with Young Tall Poppy Science AwardFighting infections relies on our immune system recognising microbes that cause disease, attacking the infection, and ‘remembering’ that infection if we encounter it again. This immune memory is crucial to giving us ongoing protection from disease, and is also key to vaccination.Dr Kim Jacobson, a researcher within the Department of Biochemistry and Molecular Biology, is an expert in understanding how we form immunity to infections. In recognition of her outstanding research and science communication to the wider community, Dr Jacobson was recently announced as a recipient of the 2016 Victorian Young Tall Poppy Science Award from the Australian Institute of Policy and Science.Dr Jacobson received the award for her research looking at the network of molecules that orchestrate the immune system’s ability to tailor its response to specific infections. Her research identified a key molecule that allows cells that produce antibodies – critical to immune memory – to survive.Dr Jacobson has also published articles for The Conversation, which were republished around the world and presented to state and federal politicians on issues facing women in science, technology, engineering, mathematics, and medicine (STEMM).Dr Jacobson said she was honoured to receive the award and excited to participate in science outreach programs open to award recipients.“The Young Tall Poppy Science Award is fantastic recognition of our research into how we form immunity and how this is subverted in disease, but also the importance of communicating that research to the public and politicians.” Dr Jacobson said.“Science illuminates the beauty of the world and the intricacies of the body, and I’m looking forward to sharing our scientific discoveries with school students and the broader community.”The Young Tall Poppy Science Award is presented to researchers with fewer than 10 years of postdoctoral experience, who combine world-class research with a passionate commitment to communicating science. Award winners spend a year sharing their knowledge with school students, teachers and the broader community through workshops, seminars and public lectures.

Recent PhD GraduatesSu-Ling Leck Thesis title: “Modulators of spinocerebellar ataxia type 3-associated ataxin-3 aggregation”Supervisors: Prof Mibel Aguilar (previously Rob Pike and Stephen Bottomley)Seong Hoong ChowThesis Title: “The role of Staphylococcus aureus Panton-Valentine leukocidin (PVL) in mammalian macrophages” Supervisors: Dr Thomas Naderer, Dr James Vince Katherine Grace LangleyThesis Title: “Investigating the role of toll-like receptor 4 in saturated fatty acid-induced inflammation” Supervisors: Prof Mark Febbraio, Graeme Lancaster

www.facebook.com/groups/NOTDRS

Page 5Department of Biochemistry and Molecular BiologyNewsletter: December, 2016

Message from the Head of Department- Professor Roger DalyDear Colleagues,With the end of 2016 fast-approaching it’s time to reflect on some of the past year’s highlights before taking a well-earned break over the holiday season. First, a number of Departmental researchers were recognised in 2016 for their outstanding achievements including: Jamie Rossjohn, who was awarded the 2016 Victorian Prize for Science and Innovation; Kim Jacobson, awarded the 2016 Victorian Young Tall Poppy Science Award; Di Yu, awarded the 2016 Milstein Young Investigator Award; Michelle Dunstone, awarded the 2016 Eppendorf Edman Award from ASBMB; and Max Cryle, who was awarded the Otto Schmeil prize.This year also saw the continued success of Departmental researchers in the NHMRC and ARC Discovery project grant systems with success rates of 28 % and 29 % respectively, approximately double the national averages. Departmental researchers were awarded a total of 15 NHMRC Project Grants, 1 NHMRC Development Grant and 5 ARC Discovery Project Grants. It was also exciting to see increased industry engagement for researchers in the department. For example, James Whisstock and Andrew Ellisdon entered into a major research collaboration with Roche to develop new treatment strategies for autoimmune disease.Congratulations also to Jamie Rossjohn who was awarded a prestigious ARC Australian Laureate Fellowship, Michael Lazarou and Jerome Le Nours who were awarded ARC Future Fellowships, Dominika Elmlund, awarded an ARC Discovery Early Career Researcher Award (DECRA), Cyril Reboul, awarded an NHMRC Early Career Fellowship and Kylie Wagstaff, awarded a Career Development Fellowship from the National Breast Cancer Foundation. Additionally, a special mention to James Whisstock who was appointed EMBL Australia Scientific Head.It has been another outstanding year for the Department with regards to publications. We increased the number of high-impact (Impact Factor >10) publications compared to last year with ground breaking publications led by Departmental researchers in the elite journals Nature Medicine, Nature Immunology, Immunity, Nature Structural Molecular Biology, and Nature Communications. This is an excellent achievement and of particular note, these high-impact publications were more broadly spread out across the department’s laboratories than in previous years.In addition to our research success we continued to see increased student intake into our undergraduate BMS and BCH units and strong honours student recruitment, which will hopefully fuel enhanced uptake into our postgraduate program. The Department also saw a record number of PhD students commencing with 26 beginning their journey in 2016. We also had 25 PhD completions in 2016, with 2 more students doing final amendments, a great result.As most will be aware, the Monash Biomedicine Discovery Institute was officially opened by the Prime Minister the Hon. Malcolm Turnbull MP on the 14th of November. The BDI complements the Department’s discipline-specific leadership in research, education and technology by consolidating disease-related research across SOBS into corresponding research programs and providing an effective interface for external engagement with industry and philanthropy. The latter interactions are particularly important in the current funding environment, and establishment of the BDI will ensure that our research maintains its competitive edge and is optimally-positioned for funding and sustainability.Finally, I’d like to thank everyone for their hard work and dedication throughout 2016, and wish you all a safe, happy and relaxing holiday season.All the best,Roger.

BDI official opening. Roger explaining our research to Prime Minister Turnbull.

CONTACT US: Department of Biochemistry and Molecular Biology Monash University, Ground Floor, Building 77, Wellington Road, Clayton VIC 3800 Australia Website: www.med.monash.edu.au/biochem Tel: +61 3 990 29400 Fax: +61 3 990 29500 Content and Layout: Brock.Conley@monash.edu

The Cutting EdgeA new feature of the Newsletter is a section focusing on recent breakthroughs in the field of biochemistry and molecular biology. These can be key conceptual developments or technological advances. Publishing in this section is open to postgraduate students and postdoctoral scientists in the Department and authors of published articles receive a $250 travel voucher to support attendance at a scientific conference. The article should be approximately 500 words long, written in a style suitable for a non-specialist reader and preferably accompanied by an explanatory figure. We will publish up to two articles in each edition of the Newsletter.Please submit articles to Brock Conley, brock.conley@monash.edu

Page 6Department of Biochemistry and Molecular BiologyNewsletter: December, 2016

SAFETY INDUCTIONS To be completed by ALL Biochem staff, students, visitors, collaborators, work experience students, etc1. Read Biochem Safety manual, prior to starting any lab/office position in Biochem. Send a signed copy of the Acknowledgement form to Safety Officer and keep a copy in your lab.2. Complete Monash OHS Online video induction, every 3 years. Take a screen shot as proof of completion and send to Safety Officer and keep a copy in your lab. (For externals, those without a Monash Authenticate, will not be able to complete this; hence continue to the next step.) (Any online generic Induction forms need not be completed as the ones in the manual are detailed specifically for the department.) http://www.monash.edu/ohs/ohs-training-and-induction/ohs-induction/ohs-induction-programs3. Complete the Lab Check list located in the Biochem Safety Manual. Send a copy to Safety Officer and keep a copy in your lab. (Update when relocating to another group, otherwise complete every 3 years) Biochem OHS Committee: Positions Vacant1.Environment Officer 2.Health & Safety Rep

OHS MATTERS

S.A.R.A.H. Online Incident and Hazard reporting systemThe link is locate in your MyMonash PortalThe S.A.R.A.H. online system is used to log ALL incidents within the first 24-48hrs no matter how trivial they seem. Hazards, when first noticed, should also be logged into the system.Lab heads can delegate all their group’s incident and hazards to the Safety Officer who will be responsible for completing the reports within the 2 week deadline. Otherwise it is the responsibility of the Lab head to do so before the 2 week deadline.If delegated to the Safety Officer, the lab head at anytime is able to read/edit the report as they see fit.

S.A.R.A.H Online Risk Assessments Start uploading Risk Assessments online via SARAH• The link is locate in your MyMonash Portal• Best to start uploading all those which need updating and also

the ones which need reviewing (every 3 years)• Tab is located within the SARAH module once logged in • Be aware that the person who uploads a risk assessment

is the ONLY person who can alter/edit that particular risk assessment

STUDENT TO STAFF CONVERSIONSRegarding transferring Training Records from Students to Staff records, provide the Safety Officer with your full list of completed training modules and the dates completed, so that they can be transferred to your staff account.

XMAS SHUTDOWN PERIOD UNIVERSITY IS CLOSED FROM 22nd Dec until 2nd Jan 2017

****ALL MEMBERS PLANNING TO WORK ON CAMPUS DURING THE XMAS SHUTDOWN PERIOD PLEASE NOTE:You are permitted to come onto campus and into the buildings as usual during the Christmas shutdown period. However, due to the limited emergency response during this period please note the following:• You must carry your ID card with you at all times. Security will be doing spot checks so keep our card with you.• You must not allow any unauthorised entry to others (ie do not let people follow you in to areas where swipe card access is required)• You must check in and check-out with security during this period.ON ARRIVAL - please notify security of your presence in the building - call 9905 3059 (put this in your mobile phone)ON DEPARTURE - please let security know that you are leaving • Your supervisor is to be informed of your intention to work during this period.• Any activities deemed to be of HIGH RISK should NOT be undertaken during this period. If you are unsure please consult with your

Supervisor and/or Safety Officer with regards to what activities are suitable.In case of an EMERGENCY contact campus security by dialling 333 from any internal phone. Alternatively you can call the emergency services (000) as usual from your mobile.Also don’t forget to:1. Clean out your Freezers/fridges!2. Declutter your lab benches, underbenches where possible3. Store away any high risk chemicals (Flammables/Corrosives/Toxics)4. Ensure all your Scheduled Poisons are locked 5. Switch off any equipment which is not in use6. Switch off DRYING/BAKING OVENS, do not leave these on over the break!7. Switch off computers which are not in use8. Keep all eyewash stations/fire extinguishers UNBLOCKED, these must be easily accessible from all angles at all times.

Reversed T Cell Receptor Docking on a Major Histocompatibility Class I Complex Limits Involvement in the Immune Response. Gras et al. Immunity. 2016 Oct 18;45(4):749-760. (Gras/La Gruta/Rossjohn Labs)

T cell receptor recognition of CD1b presenting a mycobacterial glycolipid. Gras et al. Nat Commun. 2016 Oct 27;7:13257 (Gras/Rossjohn Labs)

Liver-Resident Memory CD8+ T Cells Form a Front-Line Defense against Malaria Liver-Stage Infection. Fernandez-Ruiz et al. Immunity. 2016 Oct 18;45(4):889-902. (Caminschi Lab)

CD1a on Langerhans cells controls inflammatory skin disease. Kim et al. Nat Immunol. 2016 Oct;17(10):1159-66. IF (Rossjohn Lab)Low-dose interleukin-2 treatment selectively modulates CD4(+) T cell subsets in patients with systemic lupus erythematosus. He et al.

Nat Med. 2016 Sep;22(9):991-3. (Yu Lab)CXCR5(+) follicular cytotoxic T cells control viral infection in B cell follicles. Leong et al. Nat Immunol. 2016 Oct;17(10):1187-96. (Yu Lab)An activated form of ADAM10 is tumor selective and regulates cancer stem-like cells and tumor growth. Atapattu et al. J Exp Med. 2016

Aug 22;213(9):1741-57. (Janes Lab)G9a regulates group 2 innate lymphoid cell development by repressing the group 3 innate lymphoid cell program. Antignano et al. J Exp

Med. 2016 Jun 27;213(7):1153-62. (Zaph Lab)Accessory subunits are integral for assembly and function of human mitochondrial complex I. Stroud et al. Nature. 2016 Sep

14;538(7623):123-126. (Ryan Lab)Regulation of the P450 Oxygenation Cascade Involved in Glycopeptide Antibiotic Biosynthesis. Peschke et al. J Am Chem Soc, 2016.

138(21): p. 6746-6753 (Cryle Lab)New Structural Data Reveal the Motion of Carrier Proteins in Nonribosomal Peptide Synthesis. Kittilä et al. Angew Chem Int Ed Engl.

2016 Aug 16;55(34):9834-40. (Cryle Lab)Deciphering the Molecular Signals of PINK1/Parkin Mitophagy. Nguyen et al. Trends Cell Biol. 2016 Oct;26(10):733-44 (Lazarou Lab)Killer cell immunoglobulin-like receptor 3DL1 polymorphism defines distinct hierarchies of HLA class I recognition. Saunders et al. J Exp

Med. 2016 May 2;213(5):791-807. (Rossjohn/Vivian Labs)The intracellular pathway for the presentation of vitamin B-related antigens by the antigen-presenting molecule MR1. McWilliam et al. Nat

Immunol. 2016 May;17(5):531-7. (Rossjohn Lab)Structural basis for therapeutic inhibition of complement C5. Jore et al. Nat Struct Mol Biol. 2016 May;23(5):378-86. (Elmlund Lab)Structure of the poly-C9 component of the complement membrane attack complex. Dudkina et al. Nat Commun. 2016 Feb 4;7:10588.

(Dunstone lab)Eliminating Legionella by inhibiting BCL-XL to induce macrophage apoptosis. Speir et al. Nature Microbiology 2016 Article number:

15034 (Naderer Lab)The kinome ‘at large’ in cancer. Fleuren et al. Nat Rev Cancer. 2016 Jan 29;16(2):83-98. (Daly Lab)Diversity of T Cells Restricted by the MHC Class I-Related Molecule MR1 Facilitates Differential Antigen Recognition. Gherardin et al.

Immunity. 2016 Jan 19;44(1):32-45. (Rossjohn Lab)Atypical natural killer T-cell receptor recognition of CD1d-lipid antigens. Le Nours et al. Nat Commun. 2016 Feb 15;7:10570. (Rossjohn

Lab)Ubiquitin in the activation and attenuation of innate antiviral immunity. Heaton et al. J Exp Med. 2016 Jan 11;213(1):1-13. (Borg Lab)

Page 8Department of Biochemistry and Molecular BiologyNewsletter: December, 2016

2016 High Impact Publications (First/Last Author from Dept.)

CENTRIFUGE HIGH SPEED &/or ULTRA TRAININGFollow the instructions as per this link:https://sites.google.com/a/monash.edu/sobs-equipment-repairs/sobs---centrifuge-high-speed-ultra-training

AUTOCLAVE TRAININGThe specific link for Autoclave training is not active as yet, hence submit your training requests via the SoBS google forms using this link:https://sites.google.com/a/monash.edu/sobs-equipment-repairs/sobs-repair-form

TRAINING REQUESTS & QUERIES FOR BIOCHEM IMAGING FACILITY

For all queries, training, assistance with imaging equipment, log into the Biochem JobDesk (it is on a first come first serve basis) using this link:http://jobdesk.monash.edu.au/login/index.cfm?jobdesk_id=27For booking equipment once trained and registered, use this specific Calcium Calendar link:https://biochem-calendar.med.monash.edu.au/Calcium40.pl?Op=Splash

Notice SOBS Infrastructure Faults and

RepairsALL equipment repairs, building faults, quote requests, light failures, water leaks, lab coats, access requests, in fact anything and everything, apart from a genuine emergency, should be logged using this site:https://sites.google.com/a/monash.edu/sobs-equipment-repairs/