10
1 BK NEWS # 385 /BE/ MKT DATE : 24-05-2016 TITLE : New graphic materials for bioelisa and bioblot product lines Dear colleagues, We are pleased to present the new materials for bioelisa and bioblot product lines, which are a very useful marketing and selling tool to promote these excellent product lines. These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments (Autoblot 3000 and Autoscan PRO) in the bioblot brochure. NEWS

NEWS/media/biokit/doc/news...These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments

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Page 1: NEWS/media/biokit/doc/news...These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments

1

BK NEWS # 385 /BE/ MKT DATE : 24-05-2016

TITLE : New graphic materials for bioelisa and bioblot product lines Dear colleagues, We are pleased to present the new materials for bioelisa and bioblot product lines, which are a very useful marketing and selling tool to promote these excellent product lines. These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments (Autoblot 3000 and Autoscan PRO) in the bioblot brochure.

NEWS

Page 2: NEWS/media/biokit/doc/news...These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments

2

The available materials are listed below:

T3100-9733 Flyer bioelisa Menu T3100-9785 Leaflet bioblot

Find attached the above mentioned materials in PDF (low resolution). Please contact us if you are interested in receiving these materials in another format. We remain at your disposal for any further information you may require. Best regards,

Biokit Marketing Team Ricard Forns

OEM & Marketing Vice President [email protected] Montse Armengol

Marketing Assistant [email protected]

Ernest Mas Marketing Manager

BIO-FLASH Product Manager/Clinical Lab [email protected]

Sandra Roselló Sales Support Manager Sales Support EEMEAI

[email protected] Jordi Gómez

OEM Business Manager [email protected]

Joan Vilà Werfen Business Manager Sales Support NOA & WE

[email protected] Joaquín Ortiz

Product Manager/Blood Bank Sales Support LatAm

[email protected]

Carlos Fernández Product Manager Instrumentation

Sales Support APAC [email protected]

David Padrosa OEM Account Manager

[email protected]

Veronica Vocero Product Specialist/Blood Bank [email protected]

www.biokit.com Biokit Phone: +34-938609000 Biokit Mktg Fax: +34-938609017

Page 3: NEWS/media/biokit/doc/news...These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments

bioelisa Assay Menu

Answering your daily challenges in the laboratory

I N F E C T I O U S D I S E A S E S

bioelisa

Page 4: NEWS/media/biokit/doc/news...These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments

T310

0-97

33 R

01 M

AY-

2016

Living Immunoassay Excellence

I N F E C T I O U S D I S E A S E S1

.

bioelisaFast and efficientHighly accurateFlexible

bioelisa Assay ReactionAntigen detection Antibody detection: Immunocapture

bioelisa Assay Menu

TORCHbioelisa TOXO IgGbioelisa TOXO IgM

bioelisa RUBELLA IgG colourbioelisa RUBELLA IgMbioelisa RUBELLA IgG

bioelisa CMV IgGbioelisa CMV colour 2.0

bioelisa HSV-1 IgGbioelisa HSV-2 IgG

HIVbioelisa HIV 1+2 Ag/Abbioelisa HIV 1+2 3.0

HTLVbioelisa HTLV I+II 5.0

Hepatitisbioelisa HAVbioelisa HAV IgM

bioelisa HBsAg 3.0bioelisa HBsAg confirmatorybioelisa anti-HBsbioelisa anti-HBc

bioelisa HCV 4.0

bioelisa HEV IgGbioelisa HEV IgM 3.0

Syphilisbioelisa SYPHILIS 3.0

Chagasbioelisa CHAGAS

Helicobacterbioelisa HELICOBACTER IgG

Page 5: NEWS/media/biokit/doc/news...These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments

Answering your daily challenges in the laboratory

Simple Use

Highly Accurate

Manual or Automated

08186 Lliçà d'Amunt - Barcelona - Spain • Tel.: (34) 93 860 90 00 - Fax: (34) 93 860 90 17 e-mail: [email protected] • website: www.biokit.com

I N F E C T I O U S D I S E A S E S

3100

-978

5 R

01 M

AR-

2016

C

M

Y

CM

MY

CY

CMY

K

AF_FOLL.TRIPTICO BIOFLAHS.pdf 1 15/10/15 16:05

bioblot

bioblot HIV-1 Plus 18 Tests Cod. 3000-1467

Ordering Information

bioblot HCV 18 Tests Cod. 3000-1474bioblot HTLV 18 Tests Cod. 3000-1471Autoblot 3000 Cod. 3710-0169Autoscan PRO Cod. 3710-0368

Answering your daily challenges in the immunoblot technology

Screening tests are widely available for detecting di�erent infections. Such tests can be extremely sensitive but have a potential for being less speci�c, leading to false positive interpretations. Independent supplemental tests of high speci�city are therefore necessary to further con�rm the presence of antibodies in repeated reactive samples of screening methods.bioblot products are based on immunoblot technology and are the perfect option to con�rm infection by HIV, HCV and HTLV.

Highly Accurate• High sensitivity• High specificity• High reproducibility

Efficient• 20µl sample• 2h 15min total incubation time• Internal band to ensure sample addition• Controls included in the kit• Same incubation time for all the protocols

Flexible• Independent strips • Reagents can be used manually or automated

bioblotI N F E C T I O U S D I S E A S E S

Page 6: NEWS/media/biokit/doc/news...These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments

C

M

Y

CM

MY

CY

CMY

K

AF_FOLL.TRIPTICO BIOFLAHS.pdf 2 15/10/15 16:05

bioblot HIV-1 PlusIntended Usebioblot HIV-1 Plus has been designed for the confirmation of HIV-1 infection, and the detection of HIV-2 in samples repeated reactive when analysed by screening methods. The separated specific HIV-1 viral antigens incorporated onto the strips via electrophoresis and electrotransblot procedures, combined with a specific HIV-2 synthetic peptide on the same strip allow for further delineation of the antibody responses to specific viral proteins.

MOLECULAR WEIGHT GENE ANTIGEN

gp160 ENV Polymeric form of gp41gp120 ENV Outermembranep66 POL Reverse transcriptase p55 GAG Precursor protein p51 POL Reverse transcriptase p39 GAG Fragment of p55 gp41 ENV Transmembrane p31 POL Endonuclease p24 GAG Core protein p17 GAG Core protein HIV-2 ENV Outermembrane recombinant

PerformanceThe sensitivity of bioblot HIV-1 Plus has been evaluated in different studies showing 100% for the detection of HIV-2 and 100% for the detection of HIV-1 ENV/GAG/POL bands.Specificity has been evaluated in normal donors and sera with other viral infections, demonstrating a very good performance.

HIV

-2

Spec

ific

band

Seru

m

Cont

rol

P17

P24

P31

P39

gp41 p5

1p5

5

P66

GP

120

GP

160

bioblot HTLVIntended UseThe bioblot HTLV is intended as a supplemental antibody assay for characterising samples found repeatedly reactive by screening methods. bioblot HTLV has a high sensitivity and specificity for both the confirmation and differentiation of HTLV-I and HTLV-II seroreactivities. This is accomplished by incorporating MTA-1, a unique HTLV-1 envelope recombinant protein (rgp46-I), combined with K55, a unique HTLV-II envelope recombinant protein (rgp46-II) and rgp21, a common yet specific HTLV-I and HTLV-II epitope recombinant envelope protein.

MOLECULAR WEIGHT GENE ANTIGEN

rgp 46-I ENV Envelop recombinant rgp 46-II ENV Envelop recombinantp53 GAG Nativegp46 GAG Nativep36 GAG Nativep32 GAG Nativep28 GAG NativeP26 GAG Nativep24 GAG Nativep19 GAG Nativergp-21 ENV Envelop recombinant

PerformanceSensitivity of bioblot HTLV was studied in different performance panels, showing results from 97.1% to 99.9%, in both cases obtaining higher sensitivity values than the reference method.bioblot HTLV has a high specificity that was demonstrated both in blood donor samples and clinical specimens.

rgp2

1

p19

p24

p26

p32

p36

rgp4

6-II

p28

gp46

p53

Seru

m

Cont

rol

rgp4

6-I

bioblotI N F E C T I O U S D I S E A S E S

bioblot HCV

Intended UseBioblot HCV is a confirmatory test for the detection of HCV infection in samples previously reactive by screening methods. It is an enzyme immunoblot assay which incorporates on a same strip four HCV recombinant proteins, from the Core, NS3, NS4, and NS5 regions of the HCV genome. As the HCV proteins are expressed as GST fusion proteins, an additional GST control band is included to indicate reactivity to native GST.

GENE ANTIGEN

Core Core recombinantNS3-1 Non structural regionNS3-2 Non structural regionNS4 Non structural regionNS5 Non structural region

Performancebioblot HCV demonstrated a sensitivity >99.9% calculated in 300 HCV reactive samples, being able to detect genotypes from 1 to 6 in the BBI HCV Genotype panel. Specificity was assessed in different studies, showing high specificity both in blood donor samples and in clinical specimens from acute virall infections.

Anti-

IgG

cont

rol 3

+

Core

NS3

-1

NS3

-2

NS4 NS5

GST

co

ntro

l(n

ot v

isib

le)

IgG

co

ntro

l 1+

Autoblot 3000 Autoblot 3000 is an automated instrument for reagent dispensing, incubation with longitudinal agitation, washing and reaction stop.

Features- Allows simultaneous processing of up to 20 samples.- Disposable sample reaction tray.- Uses peristaltic pumps: Maximum reliability and precision.- Dispensing up to six different reagents per run: This feature, combined with to the standardized protocol allows the processing of two different bioblot assays in the same run.- Allows programming of up to 10 different protocol assays from the keyboard or by using an external computer.- Incubation times can be fixed or variable with a manual stop.- Safety system for recovery of the assay should a power failure occurs during the run. The instrument will prompt the user to either continue from the last step or to abort the run.

Autoscan PRO Autoscan PRO is a platform for automated interpretation of Western blot assays.

Features- A single platform programmed to interpret all Biokit immunoblots.- Analyses an entire batch of up to 36 samples/strips and generates a report in a single operation.- Correctly detects even weakly reacting proteins.- Reduces work load through comprehensive Work-List Management and reliable archiving options.- Option of easy data management feature for compliance with standard Laboratory Information Management System (LIMS).

Page 7: NEWS/media/biokit/doc/news...These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments

C

M

Y

CM

MY

CY

CMY

K

AF_FOLL.TRIPTICO BIOFLAHS.pdf 2 15/10/15 16:05

bioblot HIV-1 PlusIntended Usebioblot HIV-1 Plus has been designed for the confirmation of HIV-1 infection, and the detection of HIV-2 in samples repeated reactive when analysed by screening methods. The separated specific HIV-1 viral antigens incorporated onto the strips via electrophoresis and electrotransblot procedures, combined with a specific HIV-2 synthetic peptide on the same strip allow for further delineation of the antibody responses to specific viral proteins.

MOLECULAR WEIGHT GENE ANTIGEN

gp160 ENV Polymeric form of gp41gp120 ENV Outermembranep66 POL Reverse transcriptase p55 GAG Precursor protein p51 POL Reverse transcriptase p39 GAG Fragment of p55 gp41 ENV Transmembrane p31 POL Endonuclease p24 GAG Core protein p17 GAG Core protein HIV-2 ENV Outermembrane recombinant

PerformanceThe sensitivity of bioblot HIV-1 Plus has been evaluated in different studies showing 100% for the detection of HIV-2 and 100% for the detection of HIV-1 ENV/GAG/POL bands.Specificity has been evaluated in normal donors and sera with other viral infections, demonstrating a very good performance.

HIV

-2

Spec

ific

band

Seru

m

Cont

rol

P17

P24

P31

P39

gp41 p5

1p5

5

P66

GP

120

GP

160

bioblot HTLVIntended UseThe bioblot HTLV is intended as a supplemental antibody assay for characterising samples found repeatedly reactive by screening methods. bioblot HTLV has a high sensitivity and specificity for both the confirmation and differentiation of HTLV-I and HTLV-II seroreactivities. This is accomplished by incorporating MTA-1, a unique HTLV-1 envelope recombinant protein (rgp46-I), combined with K55, a unique HTLV-II envelope recombinant protein (rgp46-II) and rgp21, a common yet specific HTLV-I and HTLV-II epitope recombinant envelope protein.

MOLECULAR WEIGHT GENE ANTIGEN

rgp 46-I ENV Envelop recombinant rgp 46-II ENV Envelop recombinantp53 GAG Nativegp46 GAG Nativep36 GAG Nativep32 GAG Nativep28 GAG NativeP26 GAG Nativep24 GAG Nativep19 GAG Nativergp-21 ENV Envelop recombinant

PerformanceSensitivity of bioblot HTLV was studied in different performance panels, showing results from 97.1% to 99.9%, in both cases obtaining higher sensitivity values than the reference method.bioblot HTLV has a high specificity that was demonstrated both in blood donor samples and clinical specimens.

rgp2

1

p19

p24

p26

p32

p36

rgp4

6-II

p28

gp46

p53

Seru

m

Cont

rol

rgp4

6-I

bioblotI N F E C T I O U S D I S E A S E S

bioblot HCV

Intended UseBioblot HCV is a confirmatory test for the detection of HCV infection in samples previously reactive by screening methods. It is an enzyme immunoblot assay which incorporates on a same strip four HCV recombinant proteins, from the Core, NS3, NS4, and NS5 regions of the HCV genome. As the HCV proteins are expressed as GST fusion proteins, an additional GST control band is included to indicate reactivity to native GST.

GENE ANTIGEN

Core Core recombinantNS3-1 Non structural regionNS3-2 Non structural regionNS4 Non structural regionNS5 Non structural region

Performancebioblot HCV demonstrated a sensitivity >99.9% calculated in 300 HCV reactive samples, being able to detect genotypes from 1 to 6 in the BBI HCV Genotype panel. Specificity was assessed in different studies, showing high specificity both in blood donor samples and in clinical specimens from acute virall infections.

Anti-

IgG

cont

rol 3

+

Core

NS3

-1

NS3

-2

NS4 NS5

GST

co

ntro

l(n

ot v

isib

le)

IgG

co

ntro

l 1+

Autoblot 3000 Autoblot 3000 is an automated instrument for reagent dispensing, incubation with longitudinal agitation, washing and reaction stop.

Features- Allows simultaneous processing of up to 20 samples.- Disposable sample reaction tray.- Uses peristaltic pumps: Maximum reliability and precision.- Dispensing up to six different reagents per run: This feature, combined with to the standardized protocol allows the processing of two different bioblot assays in the same run.- Allows programming of up to 10 different protocol assays from the keyboard or by using an external computer.- Incubation times can be fixed or variable with a manual stop.- Safety system for recovery of the assay should a power failure occurs during the run. The instrument will prompt the user to either continue from the last step or to abort the run.

Autoscan PRO Autoscan PRO is a platform for automated interpretation of Western blot assays.

Features- A single platform programmed to interpret all Biokit immunoblots.- Analyses an entire batch of up to 36 samples/strips and generates a report in a single operation.- Correctly detects even weakly reacting proteins.- Reduces work load through comprehensive Work-List Management and reliable archiving options.- Option of easy data management feature for compliance with standard Laboratory Information Management System (LIMS).

Page 8: NEWS/media/biokit/doc/news...These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments

C

M

Y

CM

MY

CY

CMY

K

AF_FOLL.TRIPTICO BIOFLAHS.pdf 2 15/10/15 16:05

bioblot HIV-1 PlusIntended Usebioblot HIV-1 Plus has been designed for the confirmation of HIV-1 infection, and the detection of HIV-2 in samples repeated reactive when analysed by screening methods. The separated specific HIV-1 viral antigens incorporated onto the strips via electrophoresis and electrotransblot procedures, combined with a specific HIV-2 synthetic peptide on the same strip allow for further delineation of the antibody responses to specific viral proteins.

MOLECULAR WEIGHT GENE ANTIGEN

gp160 ENV Polymeric form of gp41gp120 ENV Outermembranep66 POL Reverse transcriptase p55 GAG Precursor protein p51 POL Reverse transcriptase p39 GAG Fragment of p55 gp41 ENV Transmembrane p31 POL Endonuclease p24 GAG Core protein p17 GAG Core protein HIV-2 ENV Outermembrane recombinant

PerformanceThe sensitivity of bioblot HIV-1 Plus has been evaluated in different studies showing 100% for the detection of HIV-2 and 100% for the detection of HIV-1 ENV/GAG/POL bands.Specificity has been evaluated in normal donors and sera with other viral infections, demonstrating a very good performance.

HIV

-2

Spec

ific

band

Seru

m

Cont

rol

P17

P24

P31

P39

gp41 p5

1p5

5

P66

GP

120

GP

160

bioblot HTLVIntended UseThe bioblot HTLV is intended as a supplemental antibody assay for characterising samples found repeatedly reactive by screening methods. bioblot HTLV has a high sensitivity and specificity for both the confirmation and differentiation of HTLV-I and HTLV-II seroreactivities. This is accomplished by incorporating MTA-1, a unique HTLV-1 envelope recombinant protein (rgp46-I), combined with K55, a unique HTLV-II envelope recombinant protein (rgp46-II) and rgp21, a common yet specific HTLV-I and HTLV-II epitope recombinant envelope protein.

MOLECULAR WEIGHT GENE ANTIGEN

rgp 46-I ENV Envelop recombinant rgp 46-II ENV Envelop recombinantp53 GAG Nativegp46 GAG Nativep36 GAG Nativep32 GAG Nativep28 GAG NativeP26 GAG Nativep24 GAG Nativep19 GAG Nativergp-21 ENV Envelop recombinant

PerformanceSensitivity of bioblot HTLV was studied in different performance panels, showing results from 97.1% to 99.9%, in both cases obtaining higher sensitivity values than the reference method.bioblot HTLV has a high specificity that was demonstrated both in blood donor samples and clinical specimens.

rgp2

1

p19

p24

p26

p32

p36

rgp4

6-II

p28

gp46

p53

Seru

m

Cont

rol

rgp4

6-I

bioblotI N F E C T I O U S D I S E A S E S

bioblot HCV

Intended UseBioblot HCV is a confirmatory test for the detection of HCV infection in samples previously reactive by screening methods. It is an enzyme immunoblot assay which incorporates on a same strip four HCV recombinant proteins, from the Core, NS3, NS4, and NS5 regions of the HCV genome. As the HCV proteins are expressed as GST fusion proteins, an additional GST control band is included to indicate reactivity to native GST.

GENE ANTIGEN

Core Core recombinantNS3-1 Non structural regionNS3-2 Non structural regionNS4 Non structural regionNS5 Non structural region

Performancebioblot HCV demonstrated a sensitivity >99.9% calculated in 300 HCV reactive samples, being able to detect genotypes from 1 to 6 in the BBI HCV Genotype panel. Specificity was assessed in different studies, showing high specificity both in blood donor samples and in clinical specimens from acute virall infections.

Anti-

IgG

cont

rol 3

+

Core

NS3

-1

NS3

-2

NS4 NS5

GST

co

ntro

l(n

ot v

isib

le)

IgG

co

ntro

l 1+

Autoblot 3000 Autoblot 3000 is an automated instrument for reagent dispensing, incubation with longitudinal agitation, washing and reaction stop.

Features- Allows simultaneous processing of up to 20 samples.- Disposable sample reaction tray.- Uses peristaltic pumps: Maximum reliability and precision.- Dispensing up to six different reagents per run: This feature, combined with to the standardized protocol allows the processing of two different bioblot assays in the same run.- Allows programming of up to 10 different protocol assays from the keyboard or by using an external computer.- Incubation times can be fixed or variable with a manual stop.- Safety system for recovery of the assay should a power failure occurs during the run. The instrument will prompt the user to either continue from the last step or to abort the run.

Autoscan PRO Autoscan PRO is a platform for automated interpretation of Western blot assays.

Features- A single platform programmed to interpret all Biokit immunoblots.- Analyses an entire batch of up to 36 samples/strips and generates a report in a single operation.- Correctly detects even weakly reacting proteins.- Reduces work load through comprehensive Work-List Management and reliable archiving options.- Option of easy data management feature for compliance with standard Laboratory Information Management System (LIMS).

Page 9: NEWS/media/biokit/doc/news...These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments

Answering your daily challenges in the laboratory

Simple Use

Highly Accurate

Manual or Automated

08186 Lliçà d'Amunt - Barcelona - Spain • Tel.: (34) 93 860 90 00 - Fax: (34) 93 860 90 17 e-mail: [email protected] • website: www.biokit.com

I N F E C T I O U S D I S E A S E S

3100

-978

5 R

01 M

AR-

2016

C

M

Y

CM

MY

CY

CMY

K

AF_FOLL.TRIPTICO BIOFLAHS.pdf 1 15/10/15 16:05

bioblot

bioblot HIV-1 Plus 18 Tests Cod. 3000-1467

Ordering Information

bioblot HCV 18 Tests Cod. 3000-1474bioblot HTLV 18 Tests Cod. 3000-1471Autoblot 3000 Cod. 3710-0169Autoscan PRO Cod. 3710-0368

Answering your daily challenges in the immunoblot technology

Screening tests are widely available for detecting di�erent infections. Such tests can be extremely sensitive but have a potential for being less speci�c, leading to false positive interpretations. Independent supplemental tests of high speci�city are therefore necessary to further con�rm the presence of antibodies in repeated reactive samples of screening methods.bioblot products are based on immunoblot technology and are the perfect option to con�rm infection by HIV, HCV and HTLV.

Highly Accurate• High sensitivity• High specificity• High reproducibility

Efficient• 20µl sample• 2h 15min total incubation time• Internal band to ensure sample addition• Controls included in the kit• Same incubation time for all the protocols

Flexible• Independent strips • Reagents can be used manually or automated

bioblotI N F E C T I O U S D I S E A S E S

Page 10: NEWS/media/biokit/doc/news...These materials offer an overview of the product lines, including all the bioelisa Assay Menu in the bioelisa brochure and the bioblot assay menu and instruments

Answering your daily challenges in the laboratory

Simple Use

Highly Accurate

Manual or Automated

08186 Lliçà d'Amunt - Barcelona - Spain • Tel.: (34) 93 860 90 00 - Fax: (34) 93 860 90 17 e-mail: [email protected] • website: www.biokit.com

I N F E C T I O U S D I S E A S E S

3100

-978

5 R

01 M

AR-

2016

C

M

Y

CM

MY

CY

CMY

K

AF_FOLL.TRIPTICO BIOFLAHS.pdf 1 15/10/15 16:05

bioblot

bioblot HIV-1 Plus 18 Tests Cod. 3000-1467

Ordering Information

bioblot HCV 18 Tests Cod. 3000-1474bioblot HTLV 18 Tests Cod. 3000-1471Autoblot 3000 Cod. 3710-0169Autoscan PRO Cod. 3710-0368

Answering your daily challenges in the immunoblot technology

Screening tests are widely available for detecting di�erent infections. Such tests can be extremely sensitive but have a potential for being less speci�c, leading to false positive interpretations. Independent supplemental tests of high speci�city are therefore necessary to further con�rm the presence of antibodies in repeated reactive samples of screening methods.bioblot products are based on immunoblot technology and are the perfect option to con�rm infection by HIV, HCV and HTLV.

Highly Accurate• High sensitivity• High specificity• High reproducibility

Efficient• 20µl sample• 2h 15min total incubation time• Internal band to ensure sample addition• Controls included in the kit• Same incubation time for all the protocols

Flexible• Independent strips • Reagents can be used manually or automated

bioblotI N F E C T I O U S D I S E A S E S