Nanobioanalysis: a multidisciplinar challenge

Montserrat Rivas,

José Ángel García, José Carlos Martínez

FORC technique

SPION RF biosensor

Research environment

Universidad de Oviedo

Prof. Marcos Tejedor

Magnetism & Magnetic Materials in the University of Oviedo

NanoBioAnalysis (NBA)

Oviedo University

Universitary Central Hospital

Materials Institute

NanoBioAnalysis (NBA)

Magnetic Materials Integrated

Optics

Electrochemical sensors

Electrophoresis &

Paper-based tech

Clinical Immunology

& Microbiology

Biotechnology & Biomedical

Essays

FORC technique

SPION RF biosensor

Research environment

The FORC idea

FORC: First-Order Reversal Curves

SFD: Switching Field Distribution

𝑆𝐹𝐷 𝐻ext, 𝐻𝑟 =𝜕𝑚

𝜕𝐻ext 𝐻𝑟

FORC diagram

𝜌 = −1

2

𝜕2𝑚

𝜕𝐻𝑒𝑥𝑡𝜕𝐻𝑟

J.C. Martínez-García et al., J. Phys. D: Apl. Phys. 47 (2014) 015001

Detection of non-interacting single domain particles using first‐order reversal curve diagrams

R. Egli et al, Geochemistry, Geophysics, Geosystems 11 (2010)

FORC diagrams measured on the Lake Ely sediment sample.

Magnetic and Mössbauer Spectral Study of Core/Shell Structured Fe/Au Nanoparticles

S. J. Cho et al., Chemistry of Materials 18 (2006) 960

Set of FORCs

J.C. Martínez-García et al., J. Alloy Compd. (2014)

Set of SFDs

FORC differential dissection of soft biphase magnetic ribbons

J.C. Martínez-García et al., J. Alloy Compd. (2014)

Co nanostructures in ordered templates: comparative FORC analysis

Co array samples of (a) nanowires with diameters of 65 nm, (b) nanopillars with diameters of 60 nm, and (c) nanotubes with diameters of 65 nm.

M.P. Proenca, Nanotechnology 24 (2013) 475703

FORC technique

SPION RF biosensor

Research environment

Magnetoimpedance-based detection

Magnetoimpedance

Co70Fe5Si10B15

amorphous ribbon

Samples and methods

Test fixture ribbon to impedance analyser (1-110 MHz) + Helmholz coils (dc field)

Superparamagnetic 10 nm Fe3O4 nanoparticles (SPIONs)

NPs deposited onto blotting paper strips

NPs detection with Co (GMI)

Magnetoimpedance

Co70Fe5Si10B15

amorphous ribbon

Cu-based detection

NPs detection with Cu

NPs detection with Cu

Cu-ribbon

Co-ribbon

Printed Circuit Board

Printed Circuit Board

NPs detection with PCB1

PCB1

Cu-ribbon

x 5

Meander-line circuit

NPs detection with meander-structure

Meander

PCB1

x 3

Planar spiral-like circuit

NPs detection with spiral-sensor

PCB1

Spiral

x 7

Planar spiral-like circuit

NPs detection with 5-turn spiral

5-turns

PCB1

x 30

Origin of the detection

Changing magnetic field

Induced electromotive forces

Switching magnetic moment

Skin effect

400 mm

35

mm

Active area

Cross-section of the conductor track

Proximity effect

Cross-section of three parallel traces

A frequency-related detection

Signal versus SPION mass

PWB5S

LFT: Lateral Flow Test

Sample

Conjugated SPIONs Test line

Control line

Magnetic immunoassay

LFT/SPION rf-detection

❶

❷

❸

❹

❺

❻

❶

❻

❷

❸

❹

❺

Magnetic immunoassay

Conclusions

Electromagnetic detection of SPIONs: highly sensitive, low-cost and simple fabrication/handling.

Biosensing via nanoparticles detection

Nano Bio

Sensor

Biomarkers

NPs synthesis

Size-effects

Characterization

Biotoxins Cells

Antibodies Proteins

Biointerfacing

idemm@uniovi.es

NanoBioAnalysis March 2012 http://grupos.uniovi.es/web/nba

Thank you for your kind attention