2211NE50thSt.Apt5,Seattle,WA98105M:2062148296,nbasisty@uw.eduNathanBasisty

August7,2015

SUMMARYProteomicistandcellbiologistwith5yearsofexperienceconductinglargescaleexperimentsonhighresMSinstrumentationanddevelopingbioinformaticsworkflowstoaddressquestionsinmammalianhealth/disease.ProficientinRprogrammingforhandlingandinterpretinglargebiologicaldatasets.

Establishedanewbioinformaticspipelinewhichincreasedefficiencyoflargeproteomicstudiesbymanyfold. WroteanddevelopedaseriesofRscriptsforanalysisoflargedatasets,including:optimization,filtering,

normalization,curation,databasematching,regressions,andvisualizations. ExperiencedincreatinganddeployingRapplicationsonthewebusingShiny.

Skilledinmassspectrometry(nLCMS/MS)withastrongrecordofperforminglabelfreeandlabeledshotgun(DDA)analysisofpeptidesusinghighresolutioninstrumentation

UtilizedmetaboliclabelingandMStosimultaneouslyquantifyglobalproteinabundancesandturnoverrates Developedanunprecedentedmethodofcharacterizingpolyubiquitinmediatedhomeostasisonalargescale

byintegratingbothabundanceandturnoverdatatothisenrichedsubsetofmodifiedproteins Proficientwithvariousanalysistoolsandsoftwareforlargeandsmallproteomicdatasets(seeskills). ExperienceoperatingtheLTQFTUltraandLTQOrbitrapVelosspectrometerswithananoACQUITYUPLC

chromatographysystem.Wetlaboratoryexperienceincludingproteinpreparation,activityassays,qPCR,westernsandmousework.

SKILLS

RProgramming BasicShinyapps

(nbasisty.shinyapps.io/qvalues) LCMS/MSTechniques XcaliburSoftware

SkylineSoftwareCertified TopographSoftware Proteinprep:pulldowns,

fractionation,precipitation MSDaPl(spectralcounting)

Sequest&Percolator(FDR) WesternBlots,QPCR,

EnzymeActivityAssays IngenuityPathwayAnalysis Mousework

EDUCATIONPh.D.MolecularBasisofDisease (expected)2015DepartmentofPathology,UniversityofWashington,Seattle,WAB.S.Biochemistry 2009UniversityofWashington,Seattle,WA

RESEARCHGraduateResearchAssociate(T32AG000057fellowshipaward) 20102015DepartmentofPathology,UniversityofWashington,Seattle,WAPrincipalInvestigator:PeterRabinovitch

UtilizedacombinationofMSandmetaboliclabelingacrossseveraltissuesinmicetoestimatechangesinabundancesandturnoverratesforthousandsofproteinsduringagingandseveralaginginterventions,includingcalorierestriction,rapamycin,mitochondriatargetedcatalase,andmitochondriatargetedSSpeptides.

Designedanovelproteomicmethodtoperformthefirsteverlargescaleturnoverestimationsofpolyubiquitinatedproteins,andwhilesimultaneouslydeterminingchangesinabundanceofthesemodifiedproteins.

Pioneeredtheuseofmanybioinformaticsandproteomictoolsinthelabandimplementedaworkflowwithgreatsuccesslargescalestudiesarenowroutinelydone5+timesfaster(frommonths/yearstoweeks).

Leveragedthisexpertisetowardmanyproductivecollaborationsbothwithinandoutsidethelab,includinginvestigatorsinotheruniversities.

LabTechnician/UndergraduateResearch 20082010DepartmentofPharmacology,UniversityofWashington,Seattle,WAPrincipalInvestigator:JoeBeavo

IndependentlyuncoveredactivityofphosphodiesterasevariantPDE8inmousebonemarrowderivedmacrophagesinmice,andperformedpreliminarycharacterizationofitsroleininflammation.

AssistedincharacterizationofPDE4ininflammationanditspotentialasatherapeutictargetinmousemodelsofchronicinflammatorydiseases,includingCOPDanddiabetes.

Benchwork:Doseresponsecurves,PDEactivityassays,westernblots,ELISAs,genotypingandqPCR. Mousework:specialdiets,druginjections,terminalbronchialalveolarlavages,terminalcardiacpunctures,

tissueharvests,bonemarrowextraction,tissueculture,andassistinginglucose/insulintolerancetests.MENTORING

YuxinLiu,SUNYUpstateMedicalUniversity 2014MedicalStudentTraininginAgingResearch(MSTAR)

Project:ProteostasisintheAgingLiver:InvestigationsinProteinAggregates SelectedforPosterPresentationattheAmericanMedicalAssociationResearchSymosium Selectedfororalpresentationat2015AmericanGeratricSocietyMeeting

JasonReynolds,IdahoStateUniversity 2011AmgenScholarsProgram Project:ProteinHomeostasisinAgingandLongevityAssociatedTreatmentsinMice

SELECTEDPUBLICATIONS(**cofirstauthor)PublishedKarunadharmaPP**,BasistyN**,ChiaoYA**,DaiDF,DrakeR,LevyN,RabinovitchPS(2015).RespiratoryChain

ProteinTurnoverRatesinMiceareHighlyHeterogeneousbutStrikinglyConservedAcrossTissues,Ages,andTreatments.TheFASEBJournal.

KarunadharmaPP**,BasistyN**,DaiDF,ChiaoYA,QuarlesE,CrispinD,BielasJ,EricsonN,BeyerRP,MacKayV,MacCossMJ,RabinovitchPS.(2015).SubacuteCalorieRestrictionandRapamycinDiscordantlyAlterMouseLiverProteomeHomeostasisandReverseAgingEffects.AgingCell.

DaiDF**,KarunadharmaPP**,ChiaoYA**,BasistyN**,CrispinD,HsiehEJ,ChenT,GuH,DjukovicD,RafteryD,BeyerRP,MacCossMJ,RabinovitchPS(2014).AlteredProteomeTurnoverandRemodelingbyShorttermCaloricRestrictionorRapamycinRejuvenatetheAgingHeart.AgingCell.13,529539.(winnerofAgingCellBestPaperPrize2014)

InpreparationBasistyN,DaiDF,GagnidzeA,LevyN,LemuelG,FredricksonJ,BeyerRP,HsiehEJ,MacCoss

MJ,RabinovitchPS.OverexpressionofMitochondrialTargetedCatalaseExhibitsReverseAntagonisticPleiotropyintheAgingProteome.AntioxidantandRedoxSignaling.

BasistyN,KarunadharmaPP,ReynoldsJ,LiuY,FredricksonJ,MacCossMJ,RabinovitchPS.TheChangingLandscapeofPolyUbiquitinMediatedHomeostasisinAging,CalorieRestriction,andRapamycinTreatment.

SELECTEDPRESENTATIONSOralPresentationsMitochondriaandMetabolismInterestGroupSeminar.UWSouthLakeUnion,Seattle,WA.June6th,2014.High

HeterogeneityinTurnoverRatesofRespiratoryChainProteinsisConservedacrossTissuesandTreatmentsinMice.

AmericanAgingAssociation43rdAnnualMeeting.SanAntonio,Texas.May31,2014.TheChangingLandscapeofPolyUbiquitinMediatedHomeostasisinAging,CalorieRestriction,andRapamycinTreatment.

PosterPresentationsUSHUPO10thAnnualConference.Seattle,WA.April7th,2014.HighHeterogeneityinTurnoverRatesofRespiratory

NATHANBASISTY PAGE3ChainProteinsisConservedacrossTissuesandTreatmentsinMice

GerontologySocietyofAmerica66thAnnualScientificMeeting.NewOrleans,Louisiana.Nov21,2013.MultiTissueAssessmentofMitochondrialRespiratoryChainTurnoverwithAge,CR,andRapamycinTreatment.

GRANTS/FELLOWSHIPSGeneticApproachestoAgingTrainingGrantNIAT32AG00057 2012PresentGlenAFARScholarshipforResearchintheBiologyofAging2015Present

HONORS/AWARDSAgingCellBestPaperPrize2014 2015GlenAFARScholarshipforResearchintheBiologyofAging2015HonorSocietyDeansListWhosWhoAmongStudentsinAmericanJuniorCollegesPhiThetaKappaHonorsScholarProgramVicePresidentialHonorRollRunningStartDeansList

2013201520082009200520062005200620042006200420062004200620042005

ADMINPathPresentsGraduateStudentSpeakerSelectionCommitteeAbstract/symposiumsubmissionreviewer,GerontologySocietyofAmerica

201520142015

AVOCATIONALModeratorforAskScience(http://www.reddit.com/r/AskScience)ModeratorforGerontology(http://www.reddit.com/r/Gerontology)SportsMedicineClubSecretaryStudentsforProgressiveChangememberInternationalServiceLearningProjectCulturalCafHost

201420152008200920042006200520062005200620052006

REFERENCESPeterS.Rabinovitch,MD.,PhD.UniversityofWashingtonDepartmentofPathologyBox3577051959NEPacificStreet,HSBK081Seattle,WA9819577052066853761petersr@uw.edu

GeorgeM.Martin,M.D.UniversityofWashingtonDepartmentofPathologyBox3574701959NEPacificStreet,HSBK543ASeattle,WA981952065435088gmmartin@uw.edu

MichaelMacCoss,PhDUniversityofWashingtonDepartmentofGenomeSciencesFoegeBuildingS113372015thAveNESeattle,WA9819550652063954123maccoss@uw.edu

MattKaeberlein,PhDUniversityofWashingtonDepartmentofPathologyBox3574701959NEPacificStreet,HSBD514Seattle,WA9819574702062214849kaeber@uw.edu