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8/14/2019 Multiple Seq Analysis
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Bvjq`pjf {fyvflkf
ij`hlbflqF}qfl{`nl nd pi`sx`{f {fyvflkfij`hlbflq qn i jishfs {fq
Ijjnx{ `aflq`d`kiq`nl nd piqqfsl{ iksn{{jishfs lvbcfs{ nd {fyvflkf{
Ijjnx{ cv`ja`lh nd {fyvflkf psnd`jf{ ila
m`aafl Bisent bnafj{ -ji{q xffeCi{`{ dns bnsf {npm`{q`kiqfa ilij{`{bfqmna{ `lkjva`lh pmjnhflfq`k{
8/14/2019 Multiple Seq Analysis
2/128
Bvjq`pjf {fyvflkf
ij`hlbflq f}ibpjf
Ntis [QKTJ[IPXEA"JLLPMCnqi [QKTJ[IPXEA"JLLPM[v{k [QKTJ[IPXSLFJLLDM
Mn{i [QKBJHQP"YA"DLEDM
Siln [QKBJHQP"YA"JLEDM[i{i [QKTJHEJ[YF"JMEJY
8/14/2019 Multiple Seq Analysis
3/128
Bvjq`pjf ij`hlbflq v{f{
Il `bpnsqilq dikqns `l gvah`lh qmf yvij`qnd i bvjq`pjf {fyvflkf ij`hlbflq `{
knl{`afs`lh `q{ v{fQm`{ as`tf{ qmf `lqfspsfqiq`nl nd fikmpn{`q`nl `l qmf ij`hlbflq
Qmf bvjq`pjf ij`hlbflq psnkfavsf bilnq `lkjvaf qm`{ elnxjfahf ilaqmfsfdnsf cf `lknssfkq
8/14/2019 Multiple Seq Analysis
4/128
@lqfspsfqiq`nl nd
pn{`q`nl{Hflfsijj qmfsf isf qxn `lqfspsfqiq`nl{
nd i pn{`q`nl `l i bvjq`pjf {fyvflkf
ij`hlbflq7
Ftnjvq`nlis.m`{qns`kij
Dvlkq`nlij.{qsvkqvsij
@l bil ki{f{ qmf{f isf qmf {ibf* cvq
qmf bi lnq cf,
8/14/2019 Multiple Seq Analysis
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Ftnjvq`nlis.m`{qns`kij
Fikm pn{`q`nl `l qmf bvjq`pjf {fyvflkf
ij`hlbflq sfpsf{flq{ i knl{fstfa
m`{qns`kij pn{`q`nl `l qmf a`tfshflkf nd
qmf {fyvflkf{ dsnb i knbbnl
ilkf{qns,
Fikm lvkjfnq`af ns ib`ln ik`aa`ddfsflkf `l i knjvbl `{ qmf sf{vjq nd i
bvqiq`nl nd qmiq pn{`q`nl,
8/14/2019 Multiple Seq Analysis
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Dvlkq`nlij.{qsvkqvsij
Fikm pn{`q`nl `l qmf bvjq`pjf {fyvflkf
ij`hlbflq sfpsf{flq{ i knl{fstfa
dvlkq`nlij pn{`q`nl7 tis`lh ib`ln ik`a{
`l qm`{ pn{`q`nl lftfsqmfjf{{ isf jnkiqfa
`l qmf {ibf pjikf `l qmf psnqf`l* ila
{misf qmf {ibf dvlkq`nlHflfsijj ippj`kicjf qn ib`ln ik`a{
-ila qmf knssf{pnla`lh ALI {fyvflkf
8/14/2019 Multiple Seq Analysis
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[qsvkqvsij ij`hlbflq
@l qmf `afij ki{f* xf mitf qmf qmsff"
a`bfl{`nlij {qsvkqvsf nd qmf psnqf`l{
ila kil ksfiqf qmf bvjq`pjf {fyvflkf
ij`hlbflq ci{fa nl qmf qmsff
a`bfl{`nlij {qsvkqvsf
Qm`{ `{ qmf Hnja [qilaisa nd bvjq`pjf{fyvflkf ij`hlbflq{* cvq `{ sisfj
iti`jicjf,
8/14/2019 Multiple Seq Analysis
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Xmiq `{ i hnna bvjq`pjf
{fyvflkf ij`hlbflq>I{ dns qmf ki{f nd qxn {fyvflkf{* qmf
cf{q ij`hlbflq kil cf afd`lfa i{ qmf
nlf `l xm`km qmf lvbcfs nd kmilhf{
cfqxffl qxn {fyvflkf{ `{ b`l`b`{fa
Knbpvqf ntfs ijj pi`s{ nd {fyvflkf{
dns e {fyvflkf{ qmfsf isf e-e%9.3pi`sx`{f knbpis`{nl{
8/14/2019 Multiple Seq Analysis
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Bvjq`pjf {fyvflkf
ij`hlbflq ijhns`qmb@afij ippsnikm qn bvjq`pjf {fyvflkf
ij`hlbflq `{ qn f}qfla alib`k
psnhsibb`lh,
@l{qfia nd ij`hl`lh qxn {fyvflkf{ -qxn
a`bfl{`nlij hs`a xf ij`hl e {fyvflkf{
-e a`bfl{`nlij hs`aF}qfl{`nl `{ sfjiq`tfj {qsi`hmqdnsxisa
8/14/2019 Multiple Seq Analysis
10/128
Alib`k psnhsibb`lh
dns {fyvflkf ij`hlbflq
Sfkvssflkf sfjiq`nl
Qicvjis knbpvqiq`nlQsikfcike
_i`sx`{f sfkvssflkf sfjiq`nl -sft`{`nl7
[-`*g 2 bi}U[-`"9* g"9 % b-`*g* [-`"9* g % h* [-`* g"
9 % hR
b-`*g 2 {`b`jis`q biqs`} fh CJN[VB
h 2 hip pflijq
8/14/2019 Multiple Seq Analysis
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Qmfsf isf {ftfl ki{f{xmfl ij`hl`lh qmsff
{fyvflkf{
9 3 ; 1 6 4 8@ EPHEPHEPHE"HEPHE"HE"H
G EPHEPHE"HEPHE"HEPHE"HE EPHE"HEPHEPHE"HE"HEPH
8/14/2019 Multiple Seq Analysis
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Qmsff {fyvflkf
sfkvssflkf sfjiq`nl[-`*g*e 2 bi}U
[-`"9* g"9* e"9 % b-`*g % b-`*e % b-g*e*
[-`"9* g"9* e % b-`*g % h*[-`"9* g* e"9 % b-`*e % h* [-`*g"9* e"9 % b-g*e % h*[-`"9* g* e% h % h* [-`*
g"9* e % h % h* [-`* g*
e"9 % h % hRb-`*g 2 {`b`jis`q biqs`} fh CJN[VB
h 2 hip pflijq
8/14/2019 Multiple Seq Analysis
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Qxn qn qmsff
a`bfl{`nlij hs`a
8/14/2019 Multiple Seq Analysis
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F}qfla`lh alib`k
psnhsibb`lhCi{fa nl qmf f}qsipnjiq`nl dsnb qxn qn
qmsff {fyvflkf{* xf kil afd`lf qmf
sfkvssflkf sfjiq`nl dns il lvbcfs nd
{fyvflkf{ `l qmf {ibf xi
Qmf nqmfs {qfp{ " qicvjis knbpvqiq`nl
ila qsikfcike " isf anlf `l qmf {ibfxi i{ dns pi`sx`{f ij`hlbflq
8/14/2019 Multiple Seq Analysis
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Alib`k psnhsibb`lh
q`bf `lksfi{f{f}pnlflq`ijj
Q`bf qiefl dns ij`hlbflq c alib`k
psnhsibb`lh `{ N-l ' b dns qxn{fyvflkf{ l* b kmisikqfs{ jnlh,
Q`bf qiefl dns ij`hlbflq c alib`k
psnhsibb`lh `{ N-l ' b ' p dns qmsff{fyvflkf{ l* b* p kmisikqfs{ jnlh,
8/14/2019 Multiple Seq Analysis
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Alib`k psnhsibb`lh
q`bf `lksfi{f{f}pnlflq`ijj
Kjfisj* dns e {fyvflkf{* fikm
{fyvflkf l` kmisikqfs{ jnlh* qmf q`bfsfyv`sfa x`jj cf
e N- l`
`29Qm`{ `{ f}pnlflq`ij " N- le
Xf lffa qn d`jj nvq fikm cn} `l qmf hs`a
8/14/2019 Multiple Seq Analysis
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Mfvs`{q`k bvjq`pjf
{fyvflkf ij`hlbflqXmiq {mnsqkvq{ kil xf bief>
Mnx kil xf psftflq kijkvjiq`nl q`bf
dsnb hsnx`lh f}pnlflq`ijj x`qm lvbcfs
nd {fyvflkf{>
8/14/2019 Multiple Seq Analysis
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Mfvs`{q`k bvjq`pjf
{fyvflkf ij`hlbflqKvssflqj* bn{q psikq`kij bfqmna{ isf
m`fsiskm`ij bfqmna{
Dns f}ibpjf* pi`sx`{f ij`hlbflq{*
afd`l`lh m`fsiskm dnjjnxfa c
psnhsf{{`tf iaa`q`nl nd {fyvflkf{ qn
ij`hlbflq Kjv{qij X
8/14/2019 Multiple Seq Analysis
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[qfp{ `l bvjq`pjf
ij`hlbflqKnjjfkq {fq nd {fyvflkf{ qmiq kil cf
ij`hlfa* fh ci{fa nl CJI[Q biqkmf{
Fa`q {fyvflkf{ qn knlqi`l ij`hlicjf
sfh`nl{, Fh kmsnbn{nbij {fyvflkf{
bv{q cf kvq anxl qn nlj knlqi`l hflf
sfh`nl nd `lqfsf{qSvl bvjq`pjf ij`hlbflq psnhsib
8/14/2019 Multiple Seq Analysis
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[qfp{ `l bvjq`pjf
{fyvflkf ij`hlbflqI{{f{{ ij`hlbflq nvqpvq* pn{{`cj sf"ij`hl x`qm a`ddfsflq pisibfqfs{ ns
a`ddfsflq psnhsibIagv{q ij`hlbflq bilvijj `l {nbfki{f{* pisq`kvjisj sfh`nl{ x`qm bilhip{
Bi lffa qn sfbntf vlsfjiqfa{fyvflkf{ `lkjvafa c ikk`aflq* ns fa`qsfh`nl nd {fyvflkf `lkjvafa
8/14/2019 Multiple Seq Analysis
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Kjv{qij X
_n{{`cj bn{q knbbnlj v{fa bvjq`pjf
{fyvflkf ij`hlbflq psnhsib
Ci{fa nl Dflh $ Annj`qqjf{ 90=8 `afi
nd psnhsf{{`tf ij`hlbflq
Kjv{qijX knlqi`l{ i lvbcfs nd
`bpsntfbflq{ ntfs qmf psft`nv{Kjv{qijT -90==
8/14/2019 Multiple Seq Analysis
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Kjv{qijX `l`q`ij pi`sx`{f
ij`hlbflqIjj {fyvflkf{ ij hlfa pi`sx`{f qn ijj nqmfs{
e {fyvflkf{ h`tf{ e-e%9.3 ij`hlbflq{
Kjv{qijX `lkjvaf{ i kmn`kf nd alib`kpsnhsibb`lh -{jnx.ikkvsiqf ns
X`jcvs.J`pbil -di{q.ippsn}`biqf
iaipqiq`nl nd alib`k psnhsibb`lh
Idd`lf hip pflijq`f{ kil iagv{q hip npfl`lhila hip f}qfl{`nl pflijq
8/14/2019 Multiple Seq Analysis
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X`jcvs"J`pbil di{q
pi`sx`{f ij`hlbflqSfjiqfa qn CJI[Q {fiskm`lh
Ci{fa nl f}ikqj biqkm`lh e"qvpjf{ -9*3
dns psnqf`l5 3"1 dns lvkjfnq`af
F}qfla ij`hlbflq ijnlh a`ihnlij{ x`qmbil f}ikq e"qvpjf{
Iagikflq a`ihnlij{ knl{`afsfa xmflf}qfla`lh ij`hlbflq
Ijj qmf{f pisibfqfs{ kil cf {pfk`d`fa
8/14/2019 Multiple Seq Analysis
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F{qicj`{m`lh m`fsiskm`ij
nsafs[knsf{ dsnb pi`sx`{f ij`hlbflq{ ijjnx
v{ qn ksfiqf i m`fsiskm nd qmf
{fyvflkf{
Bn{q {`b`jis {fyvflkf{ {mnvja cf
ij`hlfa d`s{q
Bi lffa {ftfsij `lqfsbfa`iqfij`hlbflq{ qmiq x`jj jiqfs cf gn`lfa
8/14/2019 Multiple Seq Analysis
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Bvjq`pjf {fyvflkf
ij`hlbflq f}ibpjf
Ntis [QKTJ[IPXEA"JLLPMCnqi [QKTJ[IPXEA"JLLPM[v{k [QKTJ[IPXSLFJLLDM
Mn{i [QKBJHQP"YA"DLEDM
Siln [QKBJHQP"YA"JLEDM[i{i [QKTJHEJ[YF"JMEJY
8/14/2019 Multiple Seq Analysis
26/128
M`fsiskm nd iaa`q`nl
Ij`hl Ntis ila Cnqi d`s{q
Ij`hl Mn{i ila Siln
Ij`hl [v{k qn Ntis ila Cnqi
Ij`hl qmf{f qxn kjv{qfs{ qn fikm nqmfs
Ij`hl [i{i qn jishf ij`hlbflq
8/14/2019 Multiple Seq Analysis
27/128
Kjv{qij X psnhsf{{`tf
bvjq`pjf ij`hlbflqF}ibpjf `jjv{qsiqf{ ijj pn{{`cjf ki{f{7
Ij`hl qxn {fyvflkf{ qn fikm nqmfs
Ij`hl i {fyvflkf qn il f}`{q`lh
ij`hlbflq
Ij`hl qxn ij`hlbflq{ qn fikm nqmfs
8/14/2019 Multiple Seq Analysis
28/128
8/14/2019 Multiple Seq Analysis
29/128
Ij`hl`lh ij`hlbflq{Nlkf {fyvflkf{ isf ij`hlfa ila hip{`lqsnavkfa* qmf{f isf lnq ijqfsfa
-m`fsiskmIj`hlbflq d`la{ i jnkij npq`bvb i{fisj ij`hlbflq afk`{`nl{ isf jnkefa `lc qmf hsffa ijhns`qmb
Fisj fssns{ x`jj cf psnpihiqfa ila bikiv{f d`lij ij`hlbflq qn cf xns{f
8/14/2019 Multiple Seq Analysis
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Kjv{qijX sfd`lfbflq{Ij`hlbflq pisibfqfs{ tis qmsnvhmnvq
ij`hlbflq psnkf{{
B-`*g bnsf a`{qilq {fyvflkf{ h`tfl
bnsf xf`hmq xmfl kijkvjiq`lh kmisikqfs
iq ` ns g
B-`*g a`ddfsflq CJN[VB biqs`} v{faci{fa nl {`b`jis`q nd {fyvflkf{
8/14/2019 Multiple Seq Analysis
31/128
Kjv{qijX sfd`lfbflq{H hip pflijq7
Jnxfs hip pflijq iq psf"f}`{q`lh hip
M`hmfs hip pflijq lfis psf"f}`{q`lh hip
Jnxfs hip pflijq `l masnpm`j`k sfh`nl{i{ qmf{f isf j`efj qn cf f}qfslij jnnp{
-psnqf`l nljHip pflijq ci{fa nl elnxl ib`ln ik`a
kmisikqfs`{q`k{
8/14/2019 Multiple Seq Analysis
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Kjv{qijX sfd`lfbflq{Lnqf qmiq qmf sfd`lfbflq{ isf bn{q
v{fdvj xmfl ij`hl`lh psnqf`l{
_snqf`l {qsvkqvsij `ldnsbiq`nl `{ tijvicjf
`l `lqfspsfq`lh {fyvflkf
Iqqfbpq qn kna`d c`njnh`kij* kmfb`kij
ila pm{`kij elnxjfahf `l ij`hlbflqijhns`qmb
8/14/2019 Multiple Seq Analysis
33/128
Kjv{qijX b`{ippj`faKjv{qij X ila nqmfs ijhns`qmb{ qmiq`lkjvaf i pi`sx`{f knbpis`{nl {qfp
bv{q lnq cf v{fa qn ij`hl {fyvflkf{qmiq an lnq ijj {misf i knbbnl cjnke
[fyvflkf{ qmiq an lnq {misf i knbbnlcjnke isf hflfsijj dsnb {fyvflkfi{{fbcj psngfkq{
8/14/2019 Multiple Seq Analysis
34/128
[fyvflkf i{{fbcj
8/14/2019 Multiple Seq Analysis
35/128
Bvjq`pjf {fyvflkf
ij`hlbflq f}ibpjf
Ntis [QKTJ[IPXEA"JLLPMCnqi [QKTJ[IPXEA"JLLPM[v{k [QKTJ[IPXSLFJLLDM
Mn{i [QKBJHQP"YA"DLEDM
Siln [QKBJHQP"YA"JLEDM[i{i [QKTJHEJ[YF"JMEJY
8/14/2019 Multiple Seq Analysis
36/128
Bvjq`pjf {fyvflkf
ij`hlbflqQmf ps`lk`pjf nd alib`k psnhsibb`lh
kil cf f}qflafa qn bvjq`pjf {fyvflkf{
Vldnsqvliqfj* qmf q`bf sfyv`sfa hsnx{
f}pnlflq`ijj x`qm qmf lvbcfs nd
{fyvflkf{ ila {fyvflkf jflhqm{
Ijhns`qmb{ `l v{f isf mfvs`{q`k ila bn{qisf psnhsf{{`tf.m`fsiskm`ij
8/14/2019 Multiple Seq Analysis
37/128
Kjv{qij XQmsff"{qfp ijhns`qmb
_i`sx`{f ij`hlbflq nd ijj pi`s{ qn
afqfsb`lf {fyvflkf {`b`jis`qAfd`lf il nsafs nd iaa`q`nl nd{fyvflkf{ qn ij`hlbflq{ ci{fa nl{`b`jis`q
Knl{qsvkq bvjq`pjf ij`hlbflqpsnhsf{{`tfj ci{fa nl afd`lfa nsafs
8/14/2019 Multiple Seq Analysis
38/128
Kjv{qij X psnhsf{{`tf
bvjq`pjf ij`hlbflqQmsff pn{{`cjf ki{f{7
Ij`hl qxn {fyvflkf{ qn fikm nqmfs
Ij`hl i {fyvflkf qn il f}`{q`lh
ij`hlbflq
Ij`hl qxn ij`hlbflq{ qn fikm nqmfs
Ijxi{ fddfkq`tfj pi`sx`{f ij`hlbflq{
8/14/2019 Multiple Seq Analysis
39/128
Ij`hl`lh ij`hlbflq{_i`sx`{f ij`hlbflq nd ij`hlbflq{ `{ ij{nkijjfa psnd`jf ij`hlbflq{
V{f alib`k psnhsibb`lh,[-`*g 2 bi}U[-`"9* g"9 % b-`*g* [-`"9* g % h*
[-`* g"9 % hR
b-`*g 2 {`b`jis`q {knsf itfsihfa ntfskmisikqfs{ iq qmiq pn{`q`nl
h 2 hip pflijq
8/14/2019 Multiple Seq Analysis
40/128
Ij`hl`lh ij`hlbflq{Nlkf {fyvflkf{ isf ij`hlfa ila hip{`lqsnavkfa* qmf{f isf lnq ijqfsfa
-m`fsiskmIj`hlbflq d`la{ i jnkij npq`bvb i{fisj ij`hlbflq afk`{`nl{ isf jnkefa `lc qmf hsffa ijhns`qmb
Fisj fssns{ x`jj cf psnpihiqfa ila bikiv{f d`lij ij`hlbflq qn cf xns{f
8/14/2019 Multiple Seq Analysis
41/128
Iagv{q`lh ij`hlbflqCfkiv{f ij`hlbflq{ isf anlf x`qm
mfvs`{q`k{ ila xf bi mitf c`njnh`kij
elnxjfahf ns v{f elnxjfahf lnq`lkjvafa `l ij`hlbflq* qmf isf ndqfl lnq
pfsdfkq
@q `{ dsfyvflqj v{fdvj qn ij`hl v{`lh{ftfsij bfqmna{ ns sfpfiq bfqmna{* ns
iagv{q ij`hlbflq bilvijj.c ff,
8/14/2019 Multiple Seq Analysis
42/128
Ikq`tf sf{fiskmQmfsf isf bil nqmfs bvjq`pjf{fyvflkf ij`hlbflq psnhsib{
Bil v{f {qsvkqvsij `ldnsbiq`nl qnknl{qsvkq il `l`q`ij psnd`jf
_nqflq`ij dns aftfjnpbflq nd lfxijhns`qmb{
Fl{vsf qsiafndd cfqxffl ijhns`qmb ilac`njnh`kij elnxjfahf
8/14/2019 Multiple Seq Analysis
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_mjnhflfq`k qsff{Mvbil
Bnv{f
Asn{npm`jiMnlf cff
DfslXmfiq
_`lfQ`bf
8/14/2019 Multiple Seq Analysis
44/128
Ftnjvq`nlis m`{qns[pfk`f{ isf sfjiqfa qmsnvhm m`{qns
J`efj nlf ns`h`l nd j`df nl fisqm
Ijj kvssflq {pfk`f{ isf vjq`biqfj
af{kflailq{ nd qmf ns`h`lij j`df
[pfk`f{ {pfk`ij`{f ila a`tfshf
hsiavijj ntfs q`bf
Aisx`l7 Ns`h`l nd [pfk`f{ -9=60
8/14/2019 Multiple Seq Analysis
45/128
[fyvflkf ftnjvq`nlHflnbf nd {pfk`f{ kiss`fa ijnlh
qmsnvhm m`{qns
[fyvflkf{ kil kmilhf7 bvqiq`nl fqk
Hflf avpj`kiq`nl ila {pfk`ij`{iq`nl
Bil hflf dvlkq`nl{ isf kflqsijj
`bpnsqilq ila isf knl{fstfa {fjfkq`nlqn bi`lqi`l psnqf`l dvlkq`nl
8/14/2019 Multiple Seq Analysis
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Hflnbf pi{{fa nlMvbil
Bnv{f
Asn{npm`jiMnlf cff
DfslXmfiq
_`lfQ`bf
8/14/2019 Multiple Seq Analysis
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[fyvflkf ftnjvq`nlBn{q c`njnh`kij {fyvflkf ilij{`{ nljxnse{ cfkiv{f xf i{{vbf ftnjvq`nl
[fyvflkf{ isf {`b`jis cfkiv{f qmf isfaf{kflailq knp`f{ -x`qm tis`iq`nl ndqmf {ibf ns`h`lij {fyvflkf
_snqf`l{ x`qm {`b`jis dvlkq`nl ila ln{fyvflkf {`b`jis`q bi {vhhf{q`lafpflaflq ns`h`l nd dvlkq`nl
8/14/2019 Multiple Seq Analysis
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Ftnjvq`nlis
sfjiq`nl{m`p{Xf x`{m qn d`la qmf ftnjvq`nlis m`{qns
-pmjnhflfq`k qsff nd i {fq nd
{fyvflkf{.bvjq`pjf ij`hlbflq,
Ndqfl qmf {fyvflkf{ isf dsnb a`ddfsflq
{pfk`f{ ila qmf qsff dsnb nd qmf
{fyvflkf{ {mnvja cf qmf {ibf i{ qmfqsff nd qmf {pfk`f{
8/14/2019 Multiple Seq Analysis
49/128
Qmf v{f nd {fyvflkf
pmjnhflXmiq `d qmf {fyvflkf{ {vhhf{q i
a`ddfsflq m`{qns qmil qmf elnxl {pfk`f{
sfjiq`nl{m`p{>
8/14/2019 Multiple Seq Analysis
50/128
Qmf v{f nd {fyvflkf
pmjnhfl_mjnhflfq`k qsff{ dsnb {fyvflkf{ kil`lksfi{f vlafs{qila`lh nd {pfk`f{
sfjiq`nl{m`p{Kil `bpsntf f{q`biqf{ nd q`bf{ nd{pfk`iq`nl ila `lqfspsfqiq`nl nd dn{{`jsfknsa
Kil `lksfi{f vlafs{qila`lh ndc`njnh`kij dvlkq`nl iq bil jftfj{
8/14/2019 Multiple Seq Analysis
51/128
@bpj`k`q m`fsiskm`f{Qsff.kjv{qfs sfjiq`nl{m`p{ i{ sfpsf{flqfa c
pmjnhflfq`k qsff{ isf `bpj`k`qj i{{vb`lh qmiq qmf
aiqi `{ sfjiqfa `l i m`fsiskm
Killnq sfpsf{flq bnsf knbpjf} qpf{ nd
sfjiq`nl{m`p{ ibnlh aiqi
Lnqf qmiq qmf Kjv{qijX ijhns`qmb i{{vbf{ i
m`fsiskm nd sfjiq`nl{m`p{ mfvs`{q`k afpfla{ nl `q
8/14/2019 Multiple Seq Analysis
52/128
F}ibpjf{ nd lnl"
m`fsiskm`ij aiqiDib`j sfjiq`nl{m`p{7 @ ib sfjiqfa qn ijj
b knv{`l{ cvq qmf isf lnq sfjiqfa qn
fikm nqmfs
8/14/2019 Multiple Seq Analysis
53/128
F}ibpjf{ nd lnl"
m`fsiskm`ij aiqiBip a`{qilkf{
8/14/2019 Multiple Seq Analysis
54/128
Lfx Mibp{m`sf -Lfx`ke)
DnsbiqMvbil
Bnv{f
Asn{npm`jiMnlf cff
DfslXmfiq
_`lf
---Mvbil* Bnv{f)* -Asn{* Cff))* -Dfsl*-Xmfiq* _`lf))5
8/14/2019 Multiple Seq Analysis
55/128
Lfx Mibp{m`sf -Lfx`ke) Dnsbiq
MvbilBnv{f
Asn{npm`ji
Mnlf cffDfsl
Xmfiq
_`lf
---Mvbil* Bnv{f)* -Asn{* Cff))* -Dfsl*
-Xmfiq* _`lf))5
8/14/2019 Multiple Seq Analysis
56/128
Lfx Mibp{m`sf -Lfx`ke) Dnsbiq
MvbilBnv{f
Asn{npm`ji
Mnlf cffDfsl
Xmfiq
_`lf
---Mvbil* Bnv{f)* -Asn{* Cff))* -Dfsl*
-Xmfiq* _`lf))5
8/14/2019 Multiple Seq Analysis
57/128
Lfx Mibp{m`sf -Lfx`ke) Dnsbiq
MvbilBnv{f
Asn{npm`ji
Mnlf cffDfsl
Xmfiq
_`lf
---Mvbil* Bnv{f)*-Asn{* Cff))* -Dfsl*
-Xmfiq* _`lf))5
8/14/2019 Multiple Seq Analysis
58/128
Sfknl{qsvkq`lh
ftnjvq`nlis qsff{ dsnbbvjq`pjf {fyvflkf
ij`hlbflq{
Qmsff kji{{f{ nd bfqmna{7
_is{`bnl
A`{qilkf bfqmna{
Bi}`bvb j`efj`mnna
Isfi nd ikq`tf sf{fiskm
8/14/2019 Multiple Seq Analysis
59/128
Sfknl{qsvkq`lh
ftnjvq`nlis qsff{ dsnbnqmfs e`la{ nd aiqi
Bnspmnjnh`kij kmisikqfs`{q`k{* `,f,
pm{`kij ippfisilkf ila iliqnbQm`{ `{ qmf qsia`q`nlij bfqmna dns
afqfsb`l`lh mnx {pfk`f{ isf sfjiqfa
Hflfsijj v{f{ pis{`bnl bfqmna
8/14/2019 Multiple Seq Analysis
60/128
Sfknl{qsvkq`lh
ftnjvq`nlis qsff{ dsnbnqmfs e`la{ nd aiqi
Hflnb`k {kijf `ldnsbiq`nl `{ lfx ila
pnqflq`ijj tfs tijvicjf7 piqqfsl{ nd{misfa hflf{ cfqxffl {pfk`f{* nsafs nd
hflf{ ijnlh kmsnbn{nbf{ fqk
Bfqicnj`k ila c`nkmfb`kij aiqi7
bfqicnj`k lfqxnse{ j`efj qn cf bnsf
{`b`jis `l kjn{fj sfjiqfa {pfk`f{
8/14/2019 Multiple Seq Analysis
61/128
Sfknl{qsvkq`lh
ftnjvq`nlis qsff{ dsnbnqmfs e`la{ nd aiqi
B`ksnissi aiqi7 xm`km hflf{ isf cf`lh
f}psf{{fa ila mnx bvkm>B`ksnissi aiqi bfi{vsf{ bSLI jftfj{
Kil knbpisf bSLI jftfj{ iksn{{
{pfk`f{ ila `l a`ddfsflq k`skvb{qilkf{fh {qsf{{ sf{pnl{f
8/14/2019 Multiple Seq Analysis
62/128
Kjv{qfs ilij{`{Lnl"ftnjvq`nlis aiqi bi ij{n cf cf{qaf{ks`cfa m`fsiskm`ijj hflfsijj
sfdfssfa qn i{ kjv{qfs ilij{`{F}ikqj qmf {ibf ps`lk`pjf{ i{ xmflknl{qsvkq`lh ftnjvq`nlis qsff{
B`ksnissi f}psf{{`nl piqqfsl{ ndqfl
ilij{fa x`qm kjv{qfs ilij{`{ qn `aflq`dhflf{ x`qm sfjiqfa f}psf{{`nl piqqfsl{
8/14/2019 Multiple Seq Analysis
63/128
Bfqmna{ dns bie`lh
ftnjvq`nlis qsff{Bfqmna{ lffa qn cf iaipqfa qn qief
ikknvlq nd fikm aiqi qpf
Lftfsqmfjf{{* bn{q bfqmna{ kil cf
kji{{`d`fa i{ pis{`bnl* a`{qilkf ns
bi}`bvb j`efj`mnna
Qmf{f bfqmna{ x`jj cf af{ks`cfa lf}qxffe dns bvjq`pjf {fyvflkf ij`hlbflq{
8/14/2019 Multiple Seq Analysis
64/128
Knl{qsvkq`lh
pmjnhflfq`k qsff{_is{`bnl
A`{qilkf bfqmna{
Bi}`bvb j`efj`mnna
Cnnq{qsipp`lh
8/14/2019 Multiple Seq Analysis
65/128
_is{`bnlNjaf{q* bn{q `lqv`q`tf bfqmna,
Dnsbijj af{ks`cfa c Mfll`h 9044
D`la qmf qsff `l xm`km qmf {bijjf{q
lvbcfs nd kmilhf{ isf lffafa qn
f}pji`l qmf nc{fstfa bvjq`pjf {fyvflkf
ij`hlbflq
8/14/2019 Multiple Seq Analysis
66/128
Mvbil HHQQIQKKQIKIQHQIQIBnv{f IKQQHQKKIIKHKHHIKISiq IKQKHQKKIIKHQHKIKI
Anh IHKQHKKQQIKHQIKIQI
Kiq IHKQHQKQQIKHQIKHQI
_is{`bnl f}ibpjf
8/14/2019 Multiple Seq Analysis
67/128
Mvbil
AnhKiq
Bnv{f
Siq
Mvbil
Bnv{fKiq
Anh
Siq
Bnv{f
AnhKiq
MvbilSiq
Kiq
Anh
MvbilBnv{f
Siq
8/14/2019 Multiple Seq Analysis
68/128
Mvbil H
Anh IKiq I
Bnv{f I
Siq I
Kn{q 9
Mvbil HHQQIQKKQIKIQHQIQI
Bnv{f IKQQHQKKIIKHKHHIKISiq IKQKHQKKIIKHQHKIKI
Anh IHKQHKKQQIKHQIKIQI
Kiq IHKQHQKQQIKHQIKHQI
8/14/2019 Multiple Seq Analysis
69/128
Mvbil H H
Anh I HKiq I H
Bnv{f I K
Siq I K
Kn{q 9 9
Mvbil HHQQIQKKQIKIQHQIQI
Bnv{f IKQQHQKKIIKHKHHIKISiq IKQKHQKKIIKHQHKIKI
Anh IHKQHKKQQIKHQIKIQI
Kiq IHKQHQKQQIKHQIKHQI
8/14/2019 Multiple Seq Analysis
70/128
Mvbil H H Q
Anh I H KKiq I H K
Bnv{f I K Q
Siq I K Q
Kn{q 9 9 3
Mvbil HHQQIQKKQIKIQHQIQI
Bnv{f IKQQHQKKIIKHKHHIKISiq IKQKHQKKIIKHQHKIKI
Anh IHKQHKKQQIKHQIKIQI
Kiq IHKQHQKQQIKHQIKHQI
8/14/2019 Multiple Seq Analysis
71/128
Mvbil H H Q Q
Anh I H K QKiq I H K Q
Bnv{f I K Q Q
Siq I K Q K
Kn{q 9 9 3 9 94
Mvbil HHQQIQKKQIKIQHQIQI
Bnv{f IKQQHQKKIIKHKHHIKISiq IKQKHQKKIIKHQHKIKI
Anh IHKQHKKQQIKHQIKIQI
Kiq IHKQHQKQQIKHQIKHQI
8/14/2019 Multiple Seq Analysis
72/128
Bnv{f I K Q Q
Anh I H K QKiq I H K QMvbil H H Q Q
Siq I K Q K
Kn{q 9 3 3 9 39
Mvbil HHQQIQKKQIKIQHQIQI
Bnv{f IKQQHQKKIIKHKHHIKISiq IKQKHQKKIIKHQHKIKI
Anh IHKQHKKQQIKHQIKIQI
Kiq IHKQHQKQQIKHQIKHQI
8/14/2019 Multiple Seq Analysis
73/128
Mvbil H H Q Q
Bnv{f I K Q QKiq I H K Q
Anh I H K QSiq I K Q K
Kn{q 9 3 3 9 39
Mvbil HHQQIQKKQIKIQHQIQI
Bnv{f IKQQHQKKIIKHKHHIKISiq IKQKHQKKIIKHQHKIKI
Anh IHKQHKKQQIKHQIKIQI
Kiq IHKQHQKQQIKHQIKHQI
8/14/2019 Multiple Seq Analysis
74/128
Kiq I H K Q
Anh I H K QMvbil H H Q Q
Bnv{f I K Q Q
Siq I K Q K
Kn{q 9 9 9 9 96
Mvbil HHQQIQKKQIKIQHQIQI
Bnv{f IKQQHQKKIIKHKHHIKISiq IKQKHQKKIIKHQHKIKI
Anh IHKQHKKQQIKHQIKIQI
Kiq IHKQHQKQQIKHQIKHQI
8/14/2019 Multiple Seq Analysis
75/128
Bn{q pis{`bnl`nv{ qsffQmf kn{q ntfs ijj pn{`q`nl{ dns i h`tfl
qsff `{ qmf qsff jflhqm
Qmfsf bi cf {ftfsij qsff{ x`qm qmf{ibf b`l`bvb qsff jflhqm
Qmf bn{q pis{`bnl`nv{ qsff `{ qmfl i
knl{fl{v{ nd qmf{f qsff{
8/14/2019 Multiple Seq Analysis
76/128
_is{`bnl
`bpjfbflqiq`nlLvbcfs nd qsff{ dns l {pfk`f{7
-3l"6) . --l";) 3l";
Knlkflqsiqf {fiskm lfis psft`nv{j"
dnvla hnna qsff{ csilkm {xipp`lh*
psvlf ila sf"hsidq
Csilkm"ila"cnvla fj`b`liqf kjfisj`ldfs`ns qsff{
8/14/2019 Multiple Seq Analysis
77/128
_is{`bnl tis`ilq{Xf kil i{{`hl a`ddfsflq kn{q{ qn kfsqi`l
kmilhf{7 fh T "< J kmfipfs qmil H "< P
Xf kil i{{`hl a`ddfsflq kn{q{ qn kfsqi`l{`qf{7 fh ;sa knanl pn{`q`nl kmfipfs
Nqmfs tis`ilq{ dns lnl"{fyvflkf aiqi
Bn{q nd qmf{f {jnx anxl knbpvqiq`nl
8/14/2019 Multiple Seq Analysis
78/128
_is{`bnl
knl{`afsiq`nl{[nbf sf{fiskmfs{ cfj`ftf `q mi{ bn{q
pm`jn{npm`kij {vppnsq -Nkkib{ siwns
Anf{ lnq afij x`qm sfpfiqfa kmilhf{ iqqmf {ibf {`qf xfjj {`bvjiq`nl{ mitf
sftfijfa jnlh csilkm iqqsikq`nl
Knbpvq`lh q`bf bi hsnx f}pnlflq`ijjx`qm bnsf aiqi* ij{n cia aiqi {jnx{ `q
8/14/2019 Multiple Seq Analysis
79/128
Jnlh csilkm iqqsikq`nlI
C
k
A
I
C
k
A
8/14/2019 Multiple Seq Analysis
80/128
A`{qilkf bfqmna{Dns {fyvflkf aiqi* i qxn"{qfp psnkf{{
Knbpvqf pi`sx`{f a`{qilkf{ cfqxffl
{fyvflkf{D`la qsff ci{fa nl a`{qilkf biqs`}7
D`qkm"Bishnj`i{m -9048 jfi{q {yvisf{
Lf`hmcns"gn`l`lh -[i`qnv $ Lf` 90=8 B`l`bvb Ftnjvq`nl -Swmfq{e $ Lf`
900;
8/14/2019 Multiple Seq Analysis
81/128
Knbpvq`lh {fyvflkf
a`{qilkf{Xf kil {`bpj knvlq a`ddfsflkf{cfqxffl {fyvflkf{ -p"a`{qilkf
Xf kil knssfkq dns bvjq`pjf kmilhf{ iqqmf {ibf pn{`q`nl7 Gvef{"Kilqns 9040
Xf kil i{{`hl a`ddfsflq kn{q{ qna`ddfsflq kmilhf{
Xf kil knssfkq dns tis`iq`nl ibnlh{`qf{7 hibbi pisibfqfs bfqmna{
8/14/2019 Multiple Seq Analysis
82/128
ALI a`{qilkf{E`bvsi 3"pisibfqfs7 knssfkq dns bvjq`pjf
kmilhf{ ila xf`hmq qsil{`q`nl{ jf{{
qmil qsil{tfs{`nl{ -9083
Tis`fq nd bnsf knbpjf} a`{qilkf{ qmiq
`lkjvaf a`ddfsflq ci{f knbpn{`q`nl{*
a`ddfsflq siqf{ nd ci{f kmilhf ila
hibbi pisibfqfs,A`{qilkf{ qmiq qief psnqf`l qsil{jiq`nl
`lqn ikknvlq
8/14/2019 Multiple Seq Analysis
83/128
_snqf`l a`{qilkf{Ci{fa nl _IB ns CJN[VB biqs`kf{
Kiqfhns a`{qilkf{ -{`b`jis ib`ln
ik`a{
Mfvs`{q`k a`{qilkf{ ci{fa nl jishf
knjjfkq`nl{ nd {fyvflkf ij`hlbflq{ ila
_IB.CJN[VB biqs`kf{Lnq i{ xfjj"aftfjnpfa i{ dns ALI
8/14/2019 Multiple Seq Analysis
84/128
D`qkm Bishnj`i{m Jfi{q
[yvisf{Knbpisf qmf nc{fstfa a`{qilkf biqs`}qn qmf a`{qilkf{ ijnlh qmf csilkmf{ nd i
pisq`kvjis qsffDns i h`tfl qnpnjnh* xf kil d`la qmfcsilkm jflhqm{ qmiq b`l`b`{f{ qmf{yvisf nd qmf a`ddfsflkf
Kijkvjiqf jfi{q {yvisf dns fikm qsffqnpnjnh* d`la cf{q qnpnjnh
8/14/2019 Multiple Seq Analysis
85/128
D`qkm"Bishnj`i{mA`dd`kvjq qn kijkvjiqf ijj csilkm jflhqm{ iq nlkf*f}kfpq dns qmsff {pfk`f{7
Csilkm ` 2 -a-`*g %a-`*e a-g*e . 3
`
g
e
8/14/2019 Multiple Seq Analysis
86/128
D`qkm"Bishnj`i{mSfavkf i qsff x`qm bnsf qmil qmsff{pfk`f{ qn a-`*g* a-`*e* a-g*e xmfsf ` ila
g isf kjn{f{q {pfk`f{* e `{ i knbpn{`qfitfsihf ntfs ijj nqmfs {pfk`f{
Sfpfiq dns lf}q"kjn{f{q {pfk`f{
F{q`biqf nd csilkm jflhqm{ dns i h`tfl
qsff qnpnjnh* qmfl d`la cf{q f{q`biqfntfs ijj qsff{
8/14/2019 Multiple Seq Analysis
87/128
Lf`hmcns"gn`l`lh[qis afknbpn{`q`nl bfqmna
Afqfsb`lf xm`km pi`s nd {fyvflkf{
sfavkf{ jflhqm nd qnqij qsff bn{q
Sfpfiq vlq`j ijj {fyvflkf{ `lkjvafa
Tfs di{q dns jishf lvbcfs{ nd
{fyvflkf{Mfvs`{q`k hsffa ijhns`qmb
8/14/2019 Multiple Seq Analysis
88/128
[qis afknbpn{`q`nl
8/14/2019 Multiple Seq Analysis
89/128
[qis afknbpn{`q`nl
8/14/2019 Multiple Seq Analysis
90/128
[qis afknbpn{`q`nl
8/14/2019 Multiple Seq Analysis
91/128
[qis afknbpn{`q`nl
8/14/2019 Multiple Seq Analysis
92/128
Lf`hmcns"gn`l`lh
ijhns`qmb[qis qsff jflhqm 2 9. -l"9 a-`*g
Dns l {pfk`f{* ntfs ijj `* g
Gn`l`lh ` ila g h`tf{ qsff jflhqm 2
9 . 3-l"3 -a-`* e % a-g* e % ^ a-`* g % 9.
-l"3 a-e* jDns l {pfk`f{* xmfsf e ila j isf ijj
{pfk`f{ nqmfs qmil ` ila g
8/14/2019 Multiple Seq Analysis
93/128
Lf`hmcns"gn`l`lh
ijhns`qmbNlkf xf mitf dnvla qmf ` ila g {pfk`f{
qmiq sfavkf qmf qnqij qsff jflhqm qmf
bn{q* sfpjikf ijj a-`*e ila a-g*e x`qmqmf itfsihf
Lnx mitf i a`{qilkf biqs`} x`qm l"9
{pfk`f{* sfpfiq psnkf{{
[qnp nlkf qmfsf isf qmsff {pfk`f{ jfdq
8/14/2019 Multiple Seq Analysis
94/128
[qis afknbpn{`q`nl
8/14/2019 Multiple Seq Analysis
95/128
B`l`bvb Ftnjvq`nl[`b`jis qn D`qkm"Bishnj`i{m b`l`b`{f{yvisf nd a`ddfsflkf cfqxffl sfija`{qilkf biqs`} ila a`{qilkf{ ijnlh
qsff,Qsff x`qm qmf {mnsqf{q qnqij csilkmjflhqm{
Lf`hmcns"gn`l`lh `{ qmf hsffa mfvs`{q`kdns d`la`lh qmf b`l`bvb ftnjvq`nl qsff
@lksfi{`lhj pnpvjis
8/14/2019 Multiple Seq Analysis
96/128
V_HBIVlxf`hmqfa _i`s Hsnvp`lh x`qm
Is`qmbfq`k Bfil
Sisfj psnavkf{ qmf knssfkq qsff
Lftfsqmfjf{{ {q`jj knbbnlj v{fa cnqm
`l pmjnhflfq`k{ ila nqmfs kjv{qfs`lh
Sfknhl`{f ila itn`a `q)
8/14/2019 Multiple Seq Analysis
97/128
V_HBID`la {bijjf{q tijvf `l a`{qilkf biqs`}*{i a-i*c
Gn`l qmf{f qxn {pfk`f{5 sfpjikf a-i*k
ila a-c*k x`qm itfsihf a`{qilkf* dns ijjnqmfs {pfk`f{ k
Sfpfiq vlq`j qsff dnsbfa
Nlj psnavkf{ knssfkq qsff `d ijjcsilkmf{ isf qmf {ibf a`{qilkf dsnbns`h`l
8/14/2019 Multiple Seq Analysis
98/128
V_HBI knvlqfsf}ibpjf
I ila Kgn`lfa d`s{q
I
C
k
A
, I C KC ,66
K ,1? ,46A ,46 ,0? ,66
,9?
,1? ,1?
,96 ,96
8/14/2019 Multiple Seq Analysis
99/128
Bi}`bvb j`efj`mnna
[qiq`{q`kij bfqmna " pnxfsdvj ila
djf}`cjf* ij{n knbpvqiq`nlijj knbpjf}
H`tfl i pisq`kvjis qsff ila i bnafj nd
qmf ftnjvq`nlis kmilhf* kijkvjiqf qmf
j`efj`mnna nd qmf qsff ci{fa nl aiqi* `,f,
qmf h`tfl bvjq`pjf {fyvflkf ij`hlbflq
Dfj{fl{qf`l 90=9
8/14/2019 Multiple Seq Analysis
100/128
Bi}`bvb j`efj`mnna
J`efj`mnna-qsff z aiqi psnpnsq`nlij qn _sncic`j`q-
aiqi z qsff
I{ `l pis{`bnl ila jfi{q {yvisf{ a`{qilkf bfqmna*
{fiskm qn d`la cf{q qsff* `,f, qsff x`qm bi}`bvbj`efj`mnna h`tfl aiqi
Bnafj nd ftnjvq`nlis kmilhf kil cf {`bpjf ns
knbpjf}* b`ssns`lh a`{qilkf bfqmna{
8/14/2019 Multiple Seq Analysis
101/128
Bi}`bvb j`efj`mnna
Qsff x`qm csilkmf{* te csilkm jflhqm{
_sncic`j`q nd kmisikqfs kmilhf _IK-q
dns I "< K `l vl`q nd q`bf q
Anlq elnx kmisikqfs {qiqf{ `l{`af qsff
-`l qmf pi{q {n kijkvjiqf dns ijj
pn{{`c`j`q`f{* f,h, I* K* H* Q
8/14/2019 Multiple Seq Analysis
102/128
Mvbil H
Anh I
Kiq I
Bnv{f I
Siq I
{?
{;
{9
{3
t1
t6
t4
t8
t=
t3
t;
t9
8/14/2019 Multiple Seq Analysis
103/128
Mvbil H
Anh I
Kiq I
Bnv{f I
Siq I
I
I
I
I
t1
t6
t4
t8
t=
t3
t;
t9
J 2 p-I) _II-t9) _II-t3) _IH-t1)_II-t6) _II-t4) _II-t;) _II-t8) _II-t=)
8/14/2019 Multiple Seq Analysis
104/128
Mvbil H
Anh I
Kiq I
Bnv{f I
Siq I
I
I
I
H
t1
t6
t4
t8
t=
t3
t;
t9
J 2 p-I) _II-t9) _IH-t3) _HH-t1)_HI-t6) _II-t4) _II-t;) _II-t8) _II-t=)
Mvbil {11
8/14/2019 Multiple Seq Analysis
105/128
Mvbil {1
Anh {6
Kiq {4
Bnv{f {8
Siq {=
{?
{;
{9
{3
t1
t6
t4
t8
t=
t3
t;
t9
J 2 p-{?) _{?{9-t9) _{9{3-t3) _{3{1-t1) _{3{6-t6)_{9{4-t4) _{?{;-t;) _{;{8-t8) _{;{=-t=)
8/14/2019 Multiple Seq Analysis
106/128
Kijkvjiq`lh _`g-T)
Siqf biqs`} nd `l{qilqilfnv{ kmilhf
Bvjq`pjf {vc{q`qvq`nl{ pfs {`qf `{
ikm`ftfa c bvjq`pj`lh `l{qilqilfnv{biqs`} c `q{fjd
_`e-t 2 _`e-`l{qilqilfnv{t
8/14/2019 Multiple Seq Analysis
107/128
@l{qilqilfnv{ ALI siqf
biqs`}, I K H Q
I 9-i9%i3%i;) i9 i3 i;
K i1 9-i1%i6%i4) i6 i4H i8 i= 9 -i8%i=%i0)i0H i9? i99 i93 9-i9?%i99%i93)
8/14/2019 Multiple Seq Analysis
108/128
ALI siqf biqs`}
Gvef{"Kilqns a`{qilkf bnafj fyv`tijflq qn ijji flqs`f{ fyvij
E`bvsi qxn"pisibfqfs fyv`tijflq qn qxntijvf{* dns qsil{`q`nl{ ila qsil{tfs{`nl
[`qf qn {`qf tis`iq`nl -j`ef hibbi a`{qilkfbnafj{ v{vijj ikm`ftfa c c`l{ nd {`qf{ x`qma`ddfsflq siqf{ nd kmilhf
Sftfs{`cjf bnafj bfil{ qmf siqf biqs`} `{{bbfqs`k
8/14/2019 Multiple Seq Analysis
109/128
F}ibpjf siqf biqs`kf{
Gvef{"Kilqns E`bvsi 3"pisibfqfs, I K H Q , I K H Q
I 9";i i i i I 9"i"3c c i cK i 9";i i i K c 9"i"3c c i
H i i 9";i i H i c 9"i"3c c
Q i i i 9";i Q c i c 9"i"3c
8/14/2019 Multiple Seq Analysis
110/128
[ppfa`lh vp bi}`bvb
j`efj`mnnaBi}`bvb j`efj`mnna anf{ cf{q `l{`bvjiq`nl cvq `{ ij{n {jnxf{q bfqmna
Tis`fq nd lfx mfvs`{q`k{ qn d`la BJ qsffdi{qfs
Kil cpi{{ {fiskm`lh ila kijkvjiqfj`efj`mnna nd {fjfkqfa.`lqfsf{q`lh qsff{
ila knbpisf nd knvs{f qmf qsvf BJqsff bi lnq cf `lkjvafa `l knbpis`{nl
8/14/2019 Multiple Seq Analysis
111/128
Kmnn{`lh i bnafj
Bnsf pisibfqfs{ af{`sicjf qn af{ks`cf{fyvflkf kmilhf ikkvsiqfj
Cvq bnsf pisibfqfs{ isf {jnxfs ilamisafs qn f{q`biqf
Fh 9??? {`qf{ `lqn dnvs hsnvp{ x`qm dnvsa`ddfsflq siqf{ nd kmilhf "< jf{{ aiqi dns
fikm kiqfhnsNtfspisibfqfs`{iq`nl
Xm` m qm a q c `ja
8/14/2019 Multiple Seq Analysis
112/128
Xm`km bfqmna qn cv`ja
b qsff x`qm>Knl{`afsicjf afciqf i{ qn xm`km `{ qmfcf{q bfqmna
A`ddfsflq bfqmna{ `bpj a`ddfsflqbnafj{ nd {fyvflkf kmilhf* a`ddfsflqpm`jn{npm`f{
@l dikq* qmf yvij`q nd qmf aiqi `{ bn{q
`bpnsqilq dikqns `ldjvflk`lh qsffknl{qsvkq`nl
8/14/2019 Multiple Seq Analysis
113/128
Yvij`q nd qmf qsff
_mjnhflfq`k qsff{ kil tis
asibiq`kijj x`qm {j`hmq kmilhf{ `l aiqi
Xf xilq qn elnx xm`km csilkmf{ isfsfj`icjf* ila xm`km csilkmf{ an lnq
mitf {qsnlh {vppnsq dsnb qmf aiqi
Cnnq{qsipp`lh `{ qmf bn{q knbbnlbfqmna v{fa -Dfj{fl{qf`l 90=6
8/14/2019 Multiple Seq Analysis
114/128
Cnnq{qsipp`lh
Cnnq{qsipp`lh `{ i hflfsij {qiq`{q`kijqfkml`yvf dns afqfsb`l`lh mnx bvkm
fssns `{ `l i {fq nd sf{vjq{Cnnq{qsipp`lh `{ ippj`kicjf `d f`qmfs7
Xf isf lnq {vsf nd qmf {ibpjf {pikfqmf aiqi `{ asixl dsnb
Qmf bfqmna ippj`fa qn qmf aiqi killnqcf fi{`j ilij{fa
8/14/2019 Multiple Seq Analysis
115/128
Cnnq{qsipp`lh
_{fvansfpj`kiqf{ nd aiqi {fq
Silanbj asix `qfb{ dsnb aiqi {fq x`qm
sfpjikfbflq* qn {`wf nd ns`h`lij aiqi {fqKijkvjiqf af{`sfa sf{vjq dns fikm
p{fvansfpj`kiqf
F}ib`lf a`{qs`cvq`nl nd sf{vjq iksn{{jishf {fq nd p{fvansfpj`kiqf{
C q q `
8/14/2019 Multiple Seq Analysis
116/128
Cnnq{qsipp`lh {fyvflkf
ij`hlbflq{Dns fikm p{fvansfpj`kiqf* asixknjvbl{ nd aiqi silanbj x`qm
sfpjikfbflq qn ksfiqf i bvjq`pjf{fyvflkf ij`hlbflq nd {ibf jflhqm
Knl{qsvkq pmjnhflfq`k qsff dns fikm*v{`lh {ibf bfqmna ila pisibfqfs{
Afs`tf knl{fl{v{ qsff* knbpisf qnns`h`lij qsff
8/14/2019 Multiple Seq Analysis
117/128
F}ibpjf ij`hlbflq
9999999993;1648=0?93;1648
Ntis [QKTJ[IPXEAFJLLPMCnqi [QKTJ[IPXEA"JLLPM[v{k [QKTJ[IPXSL"JLLDM
Mn{i [QKBJHQP"YA"DLEDMSiln [QKBJHQP"YA"JLEDM[i{i [QKTJHEJ[YF"JMEJY
S a c `qm
8/14/2019 Multiple Seq Analysis
118/128
Silanb lvbcfs{ x`qm
sfpjikfbflq@l qmf f}ibpjf* qmfsf isf 98 pn{`q`nl{ `l
qmf ij`hlbflq
Kmn{f i silanb lvbcfs cfqxffl 9ila 98* 98 q`bf{
[nbf x`jj cf kmn{fl {ftfsij q`bf{*
{nbf lnq iq ijj6 9? 98 9? 8 9; 96 6 3 8 8 = 9 9? = 8 99
8/14/2019 Multiple Seq Analysis
119/128
99999999
93;1648=0?93;1648Ntis [QKTJ[IPXEAFJLLPM
Cnqi [QKTJ[IPXEA"JLLPM
[v{k [QKTJ[IPXSL"JLLDMMn{i [QKBJHQP"YA"DLEDMSiln [QKBJHQP"YA"JLEDM
[i{i [QKTJHEJ[YF"JMEJY99 3 13 ;9 9 9 9
8/14/2019 Multiple Seq Analysis
120/128
8/14/2019 Multiple Seq Analysis
121/128
Knl{fl{v{ qsff
9??? p{fvansfpj`kiqf{ "< 9??? qsff{
Knl{fl{v{ qsff7 @aflq`d csilkmf{
psf{flq `l ijj qsff{ d`s{qQmfl d`la csilkm {ffl `l bn{q qsff{
D`la lf}q bn{q knbbnl csilkm*knlq`lvf
An lnq `lkjvaf csilkmf{ qmiq knldj`kqx`qm psft`nv{j"iaafa csilkmf{
8/14/2019 Multiple Seq Analysis
122/128
Knl{fl{v{ qsff
I
C K
A
FFF
F
A
AA
K
KC
C
CC
C
C
C
C
C
K
K
K
K
K
K
K
II
I
I
I
I
I
I
I
A
A
A
A
A
A
F
F
F
F
FF
88&
66&
Xmiq an qmf cnnq{qsip
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123/128
Xmiq an qmf cnnq{qsip
tijvf{ bfil>Cnnq{qsip tijvf{ dns pmjnhflfq`k qsff{ an lnq dnjjnxpsnpfs {qiq`{q`kij cfmit`nvs
Cnnq{qsip tijvf 06& ikqvijj kjn{f qn 9??&
knld`aflkf `l qmiq csilkmCnnq{qsip tijvf 86& ndqfl kjn{f qn 06& knld`aflkf
Cnnq{qsip tijvf 4?& `{ bvkm jnxfs knld`aflkf
Jf{{ qmil 6?& cnnq{qsip7 ln knld`aflkf `l qmiqcsilkm ntfs il ijqfsliq`tf
8/14/2019 Multiple Seq Analysis
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Cnnq{qsip j`b`qiq`nl
Cfkiv{f xf isf sf{ibpj`lh dsnb qmf
f}`{q`lh aiqi {fq* xf killnq ftfs mitf
p{fvansfpj`kiqf{ x`qm knjvbl{ lnqnc{fstfa `l qmf ns`h`lij aiqi {fq
Bi {nbfq`bf{ jfia qn ntfsf{q`biq`nl
nd cnnq{qsip tijvf dns pisq`kvjis csilkm
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125/128
8/14/2019 Multiple Seq Analysis
126/128
_sikq`kij knl{`afsiq`nl{
Cfkiv{f LG `{ tfs di{q ila h`tf{ psfqqhnna qsff{* v{f LG qn qf{q a`ddfsflqpisibfqfs{ ila cnnq{qsipp`lh
Ij{n qf{q a`ddfsflq ij`hlbflq{ qn {ffmnx bvkm qmf ij`hlbflq iddfkq{ qmf qsff
V{f Bi}`bvb J`efj`mnna dns bn{q
sfj`icjf qsff5 ns qn knbpisf {fjfkqfaqsff{
8/14/2019 Multiple Seq Analysis
127/128
_mjnhflfq`k{ {ndqxisf
_MPJ@_ Gnf Dfj{fl{qf`l bn{q v{fa{ndqxisf, Dsff* bnavjis* jishf tis`fq ndbfqmna{
_IV_' " Ait`a [xnddnsa Ds`flaj`fsv{fs `lqfsdikf* ns`h`lijj gv{q pis{`bnl*lnq dsff
BFHI3 [vam`s Evbis Dsff*{qsflhqm `l lf`hmcns"gn`l`lh* _K{ nlj
8/14/2019 Multiple Seq Analysis
128/128
_mjnhflfq`k{ {ndqxisf
Bil {pfk`ij`{q pikeihf{ lfxbfqmna{ `bpjfbflqfa c ivqmns{
Bi mitf a`ddfsflq `lpvq ila nvqpvqdnsbiq{
F}qfl{`tf `laf} iq
mqqp7..ftnjvq`nl,hflfq`k{,xi{m`lhqnl,fav.pmj`p.{ndqxisf,mqbj