Mucosal immunity

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Mucosal immunity. Epithelial barriers. Skin Mucosa. Skin 3 m 2 Mucosa 300 m 2 ↑ Ag pressure ( food , bacteria of GIT..) 80% imunoactive cells 90% infections 5-9 g IgA / day. Mucosal immunity. Tasks Infectious diseases defense Tolerance of innocent Ag. - PowerPoint PPT Presentation

Text of Mucosal immunity

Znt

Mucosal immunityEpithelial barriersSkinMucosa

Skin3 m2

Mucosa300 m2 Ag pressure(food,bacteria of GIT..)80% imunoactive cells90% infections 5-9 g IgA /dayMucosal immunityTasks

Infectious diseases defense

Tolerance of innocent Ag

Pirozen imunita

Epiteliln bukyF-CE:Trvic (enzymy)

Ochrann Antimicrobiln peptidy (Lysosym, Lactoferin, Lactoperoxidza,Defensiny 20-100AA)Meditory zntuCytokiny, receptory pro cytokinyMHC, Adhesivn m. PRRpIgAR transport IgA na sliznin povrch

Moicroflora host symbiosisColonization rezistanceEpithelial cells activationImmune system development

? Why comensal bacteria dont induce inflammation under physiologic conditionsAg uptakeDCM cells.EnterocytesMacrophages

Ag presentationProffessional APC : DC, B cells, Macrophages

Innocent Ag presented by immature DC (CD80,86), IL-10, TGF-, Th2, Th3 Oral tolerancePathogen presented by mature DC ( CD80,86), IL-12Inflammation

Funkn struktura MALTMsta indukce im. odp. organizovan lymfatick tk

GIT - Peyersk plty , FAE + geerminln centra s T, B,DCRespiran trakt Waldayerv mzn okruh

Recirculace

Homing47 integrin (homing receptor) MadCAM 1(adresin)

Efektorov tkn difuzn lymfatick tk ve slizniciLPL (lamina propria lymphocytes): CD4+ T bb, plazmatick bb. IgA+ (IgM, IgG)

IEL (intraepithelial lymphocytes): CD8+ T bb, extrathymickho pvodu ? dohled nad integritou epitelu

Common mucosal immune systemSpolen sliznin imunitn systm

IgA transport

IgA properties and functionProteases resistanceNeutralization of viruses and toxins (also IC)Microbs Adherence and invasion inhibitionOpsonizationADCCAntiinflammatory effect

Oral toleranceHigh doses of Ag - Anergy,deletion of autoreactive lymphocytesLow doses of Ag - Active supression, inhibitory cytokines production (TGF-)Experimental modelsRASMDM

Mucosal vaccines

Replicating AgCorpuscular Ag aimed to M cells vectors (liposomes, micropatticles)Epithelial cell binding proteins (cholera toxin)

Sliznin imunitaHoming: Lymfocytrn integrin 47Endoteliln ligand Mad CAM 1

Fixace IEL v epiteluLymfocytrn integrin E7Epitel sliznic: E cadherinAntiinflammatory drugsCorticosteroids: decrease of circulating lymphocytes, lympholysisReduce phagocyting and killing ability of macrophages and neutrophilsStabilize lysosomal membraneReduce chemotaxisReduce expression of MHCIIReduce production of IL-1 in macrophagesNonsteroid antiinflammatory drugs:Reduction of arachidonic acid products