Innovation. Pure & Simple.

Most Popular Products

Who We Are

Since its inception in 1994, Zymo Research has been proudly serving the scientific community by providing innovative, reliable, and high quality research tools at affordable prices. Our vision “The Beauty of Science is to Make Things Simple” is now truer than ever. Whether it’s epigenetics, DNA, RNA, E. coli, or yeast based research, our philosophy remains the same: To provide the highest quality products in the industry while ensuring they are both simple to use and reliable in their performance.

Zymo Research stands on three pillars which form the foundation of our company: Innovation, Quality, and Customer Service. These pillars are fundamental to our culture and ensure our products meet your needs.

Innovation

Zymo Research is historically recognized for its innovation of high quality nucleic acid purification technologies. Under the branding DNA Purification Made Simple® and RNA Purification Made Simple®, our technologies are pushing the limits of what is possible with nucleic acid isolation. As The Epigenetics Company™, Zymo Research has also received much attention for its rapidly expanding portfolio of epigenetics products and services. It is our objective to develop and provide the most comprehensive set of tools for DNA, RNA, and epigenetic research and analysis available today. Thousands of peer-reviewed scientific publications from researchers around the world feature our technologies. Through innovation, our scientists have made streamlined DNA methylation detection possible, pioneered the micro-elution column for DNA and RNA purification, developed the simplest and the most sophisticated methods for high-quality plasmid DNA purification, and patented the first RNA purification directly from Trizol® without phase separation among many other leading technologies in the industry.

Quality

We are committed to quality and guarantee that all of our products and service will meet and exceed your expectations. Our products are constantly evaluated by scientists like you to help ensure their reliability and the highest standard of quality.

Customer Service

We strive for excellence in how we support your scientific endeavors. We pledge to be honest and responsible for everything we do with you. We will treat you as we would like to be treated. Together, we will build a brighter future.

Contents

Epigenetics

EZ DNA Methylation-Lightning® KitChIP DNA Clean & Concentrator® Kit

16

DNA PurificationZymoPURE™ Plasmid Kits

DNA Clean & Concentrator® -5 Kit Zymoclean™ Gel DNA Recovery Kit

Quick-DNA™ Miniprep Plus Kit Quick-cfDNA™ Serum & Plasma Kit

8 - 11

RNA Purification

Direct-zol™ RNA Miniprep Kit Quick-RNA™ Miniprep Plus Kit RNA Clean & Concentrator™-5

4 - 6

DNA/RNA Co-Purification

Quick-DNA/RNA™ Miniprep Plus

7

Sample Collection and Stabilization

DNA/RNA Shield™ Collection DevicesDNA/RNA Shield™ Reagent

2 - 3

MicrobiomicsZymoBIOMICS® Microbial Community Standards

ZymoBIOMICS® DNA Miniprep Kit Quick-DNA™ Fecal/Soil Microbe Miniprep Kit

Quick-16S™ NGS Library Prep Kit

13 - 15

E. coli

Mix & Go!™ Competent Cells (DH5 Alpha)

12

2

Sample Collection and Stabilization

Product Cat. No. Size

DNA/RNA Shield™ R1100-50 50 ml R1100-8-S (8 ml)

DNA/RNA Shield™ (2X Concentrate) R1200-25 25 ml –

DNA/RNA Shield™

• Nucleic acid preservation (at ambient temperature; cold-free).• Pathogen inactivation (bacteria, fungus, parasites & viruses).• Streamlined purification (no reagent removal, universally compatible, automatable).

0

40

30

20

10

Stool

0 7 14 30Days

0

5

10

15

20

25

30

35

40

Day 0 Day 7 Day 14 Day28

Aver

age

Ct

fecal

RNA

DNA

RNA

DNA

Ct

Viruses, bacteria and yeast are effectively inactivated by DNA/RNA Shield™. Samples containing the infectious agent (virus, bacteria, yeast) were treated for 5 minutes with DNA/RNA Shield™ or mock (PBS). Titer (PFU) was subsequently determined by plaque assay. Validated by: Influenza A - D. Poole and Prof. A. Mehle, Department of Medical Microbiology and Immunology, University of Wisconsin, Madison; Ebola (Kikwit) - L. Avena and Dr. A. Griffiths, Department of Virology and Immunology, Texas Biomedical Research Institute; HSV-1/2 - H. Oh, F. Diaz and Prof. D. Knipe, Virology Program, Harvard Medical School; E. coli, L. fermentum, B. subtilis, S. cerevisiae – Zymo Research).

*Disclaimer: This graph only displays results from E. coli inactivation. Each microbe was tested independently and were combined into one graph for brevity. Bacterial cultures were grown between 108 - 109 cells and yest cultures were grown between 107 - 108 cells.

No Reagent Removal. Simple, direct purification using Zymo Research Purification Products*.

*Also compatible with most other commercial products.

Sample inDNA/RNA Shield™ Bind directly

Eluted DNA/RNA

Simply add binding reagent

to a spin-column or Magbeads

is ready to use

DNA and RNA in stool is effectively stabilized in DNA/RNA Shield™ at ambient temperature. Graphs show: spike-in DNA and RNA controls from stool purified at the indicated time points and analyzed by (RT)qPCR.

SAMPLES AVAILABLE

1

3

7

9

5

PBS

HSV

Ebo

la

Influenza

E. c

oli

*

B. s

ubtil

isL.

ferm

entu

mE.

faec

alis

M. t

uber

culo

sis

L. m

ono

cyto

gen

esP.

aer

ugin

osa

S.

ent

eric

aS.

aur

eus

DNA/RNA Shield™

Bacteria YeastVirus

viru

s: lo

g p

fu/m

l

bac

teri

a/ye

ast:

log

cfu

Complete Inactivation

C. n

eofo

rman

sC

. alb

ican

sS.

cer

evis

iae

*

Sam

ple

Co

llect

ion

& S

tab

iliza

tion

3

DNA/RNA Shield™ Collection Devices

Blood Collection Tube

Evacuated blood tube (16 x 100 mm) filled with 6 ml of DNA/RNA Shield™ for the direct collection of 3 ml whole-blood (human or animal).

Fecal Collection Tube

15 ml container (prefilled with 9 ml DNA/RNA Shield™) for the direct collection of up to 1 gram and/or 1 ml stool. Collection spoon is included

in the container screwcap.

Microbe & Tissue Lysis Tubes

2 ml collection tube (prefilled with 1 ml DNA/RNA Shield™) and BashingBeads™ for the collection and homogenization of microbial or

tissue samples.

Swab and Collection Tube

5 ml screwcap container (12 x 80 mm) filled with 1 ml DNA/RNA Shield™ and a sterile swab for specimen collection.

Product Cat. No. Size

DNA/RNA Shield™ - Blood Collection Tube R1150 50 pack –

DNA/RNA Shield™ - Fecal Collection Tube R1101 10 pack –

DNA/RNA Shield™ Lysis Tube (Microbe) R1103 50 pack –

DNA/RNA Shield™ Lysis Tube w/ Swab (Microbe) R1104 50 pack –

DNA/RNA Shield™ Lysis Tube (Tissue) R1105 50 pack –

DNA/RNA Shield™ - Swab & Collection TubeR1106R1107

10 pack (1 ml fill)50 pack (1 ml fill)

R1106S (10 pack)

SAMPLES AVAILABLE

Samp

le Co

llection &

Stabilizatio

n

4

Product Cat. No. Size

Direct-zol™ RNA Miniprep Kit R2050 50 preps –

Direct-zol™ RNA Miniprep Plus Kit R2070 50 preps R2070S (10 preps)

RNA: Purification

Direct-zol™ RNA Miniprep Kit

TRIzol® and TRI Reagent® are registered trademarks of Molecular Research Center, Inc.

U.S. Patent No. 9,051,563 B2 and other pending patents. Direct-zol™ is a trademark of Zymo Research Corp.

• Easy Handling: No phase separation or precipitation steps.• NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).• Non-Biased: Complete RNA recovery without miRNA loss.

SAMPLES AVAILABLE

RNA purified from TRIzol® using Direct-zol™ RNA compared to an unbiased method (mirVana™, Ambion). Data is highly correlated (2837 overlapped miRNA: r2 = 0.9706). Analysis was performed using miRNA-Seq (MiSeq®, Illumina).

Direct-zol™ RNA kits recovered ~4-fold more miRNAs (< 40 nt) than conventional methods. miRNAs purified from cells and tissue were quantified using Bioanalyzer small RNA chip.

Non-Biased miRNA Recovery Highest Yields

Unb

iase

d M

etho

d (l

og

10)

Direct-zol (log10)

miRNA-Seq

2837 overlapped miRNA: r2 = 0.9706

High-quality intact small and large RNAs are efficiently recovered using a Direct-zol™ RNA kit compared to using a Supplier Q kit. RNA is DNA-free and ready for all downstream applications including NGS.

NGS-Ready RNA

Genomic DNAContamination

Small RNAs

1

10

100

80

6040

20

[kb]

[nt]

Direct-zol™ Supplier Q

Cells

mic

roRN

A (p

g)

mic

roRN

A (µ

g)

Conventional Method

10,000

8,000

6,000

4,000

2,000

0Conventional

Method

Tissue (liver)3

2

1

0Direct-zol™ Direct-zol™

RNA

5

Product Cat. No. Size

Quick-RNA™ Miniprep Plus Kit R1057 50 preps

Quick-RNA™ Miniprep Plus Kit

• Broad Range: Extract total RNA (including small/micro RNAs) from any sample.• DNA-Free: Genomic DNA removal column and DNase I included.• NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.

RNA isolated with Quick-RNA™ is DNA-free compared to a Supplier Q kit. Total RNA was isolated from 106 human epithelial cells (with in-column DNase treatment for both kits, n=3).

The Quick-RNA™ Miniprep Plus Kit is universal and accommodates any sample input including cultured cells, any tissue, whole blood, tough-to-lyse samples, etc.

High-quality RNA was consistently isolated with the Quick-RNA™ Miniprep Plus Kit (left), but not with Supplier Q kits (right). Quality was assessed using the Agilent 2200 TapeStation® system; red = low quality.

DNA-Free RNA

Universal Sample Compatibility

NGS-Ready RNA

Mammalian Cells Solid Tissue Whole Blood Bacteria, Yeast, Plant

25

HeL

a

HC

T116

WB

C

Live

r

Lung

Sple

en

Bra

in

Mus

cle

Hea

rt

Hum

an

Porc

ine

E. c

oli

L. fe

rmen

tum

S. c

erev

isia

e

Nic

otia

na s

p.

200

500

1000

2000

40006000

—— Quick-RNA™

- - - - Supplier Q

RT/PCR

PCR

RFU

Cycles

20

0

200

100

300

400

30 40

Live

r

Lung

Sple

en

Bra

in

Mus

cle

Hea

rt

Live

r

Lung

Sple

en

Bra

in

Mus

cle

Hea

rt

Quick-RNA™ Miniprep Plus Kit Kit 1 Kit 2 Kit 3Zymo Research Supplier Q

25

200

500

1000

2000

40006000

RNA

6

Product Cat. No. Size

RNA Clean & Concentrator™-5 R1015 50 preps R1015S (5 preps)

RNA Clean & Concentrator™-5 w/ DNase I R1013 50 preps –

RNA Clean & Concentrator™-5

• NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.• Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.• Maximum Recovery: Recover >90% and elute in as little as 6 μl.

RNA: Clean-Up

SAMPLES AVAILABLE

Based on cited journal articles, 67% of all RNA Clean & Concentrator™ (RCC™) journal citations are used for library preparation, and 52% of the same set of citations are used for Illumina protocols and workflows.

Widely Used for NGS

RCC™Usage

67%used for library prep

52%used in Illumina protocols

RNA was cleaned-up using the RCC™ kit and a Supplier Q kit (n=4). RNA purity (measured by A260/230) was greater than 1.8 for the RCC™ kit but not for the Supplier Q kit.

Increasing amounts of RNA was cleaned up using the RCC™ kit and a Supplier Q kit (n=2). RCC™ provides higher yields and more consistent recovery when compared to the Supplier Q kit.

Ultra-Pure High Recovery Rate

Zymo Research RCC™

1.2

1.8

1.4

2

1.6

2.2

2.4

Purity (A260/230)

Supplier Q

Zymo Research RCC™

RNA Input (µg)

0.2 10

20

40

60

80

100

5 10

RNA

Rec

ove

ry (%

)

Supplier Q

RNA

7

DNA/RNA Co-Purification

Product Cat. No. Size

Quick-DNA/RNA™ Miniprep Plus Kit D7003 50 preps

Quick-DNA/RNA™ Miniprep Plus

• Highest Yields: Efficient isolation of genomic DNA and total RNA (including miRNA) from any sample source.• NGS-Ready: DNA/RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.• Single-Cell Detection: Ready for high sensitivity assays down to a single-cell (6 µl elution volume).

The Quick-DNA/RNA™ Plus technology is universal and accommodates any sample input including cultured cells, any tissue, blood, tough-to-lyse samples, etc.

Universal Sample Compatibility

Mammalian Cells

Solid TissueWhole Blood

Bacteria, Yeast, Plant

25

HeL

a

HC

T116

WB

C

Live

r

Lung

Sple

en

Bra

in

Mus

cle

Hea

rt

Hum

an

Porc

ine

E. c

oli

L. fe

rmen

tum

S. c

erev

isia

e

Nic

otia

na s

p.

200

500

1000

2000

40006000

100

250

400

600

900120015002000250030004000

70001500048500

RNA

DN

A

bp

nt

DNA and total RNA recovery is higher using the Quick-DNA/RNA™ Microprep Plus Kit compared to a Supplier Q kit. Nucleic acids were extracted from 50K HeLa cells (n=2).

HeLa cells were titrated down to a single cell, and DNA and total RNA were extracted using the Quick-DNA/RNA™ Microprep Plus Kit. Analysis by RT-qPCR shows high linear recovery of DNA & RNA down to the single-cell level (n=2).

Highest Yields Single-Cell Detection

DNA0

0.2

0.4

0.6

0.8

1

Yiel

d (µ

g)

Zymo Research Supplier Q

RNACell Number Input

0

5

10

15

20

25

35

30

Ct

1 10

DNARNA

100 1,000 10,000 100,000 1,000,000

R2 = 0.9952

R2 = 0.9859

DN

A/RN

A C

o-p

urification

8

DNA: Genomic DNA Isolation

Quick-DNA™ Miniprep Plus Kit

• Quick & Easy: Simple 20 minute procedure. • Highest Yield: Recover 3x more DNA.• Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.

Quick-cfDNA™ Serum & Plasma Kit

• High Processing Volume: Purify ≤ 10 ml of serum or plasma and elute with 35 µl.• Highest Yields: Consistently purify > 30% more cfDNA.• Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, etc.

M Quick-DNA™ QIAamp® QIAamp® QIAamp® M

Whole Blood Cells Muscle Tissue

Quick-DNA™ Quick-DNA™

The Quick-DNA™ Miniprep Plus Kit yields highly concentrated DNA without RNA contamination when compared to the QIAamp® (Qiagen) utilizing porcine whole blood, HeLa cells, and bovine muscle tissue. The resulting DNA from each sample was analyzed in a 1% (w/v) TAE/agarose/EtBr gel.

Cell-free DNA recovery scales linearly up to 10 ml using the Quick-cfDNA™ Serum & Plasma Kit.(A) Graphs and (B) gel image show the linear recovery of cfDNA from human plasma and serum (healthy female donors), as measured by Agilent 2200 TapeStation® (in duplicates).

Product Cat. No. Size

Quick-DNA™ Miniprep Plus Kit D4068 50 preps D4068S (10 preps)

Quick-cfDNA™ Serum & Plasma Kit D4076 50 preps –

SAMPLES AVAILABLE

A) Plasma/Serum

cfD

NA

[ng

/µl]

2

01 5 10 ml

R2=0.99 (36yr F serum)

R2=0.97 (58yr F serum)

R2=0.99 (54yr F plasma)

R2=0.99 (36yr F plasma)

R2=0.97 (58yr F plasma)

B)

36yr F plasma

100

200

300

400

500

700

1,000

1,500

[bp]

1 ml 5 ml 10 ml

DN

A

9

DNA Clean & Concentrator® -5 Kit

DNA: Clean-Up & Gel DNA Recovery

• Fast & Simple: Clean and concentrate DNA in 2 minutes.• Highly Concentrated: Elute in as little as 6 µl.• Ultra-Pure: Ready for Next-Gen Sequencing, PCR, ligations, etc.

Zymoclean™ Gel DNA Recovery Kit

• Superior Yields: Recover 80% of DNA from agarose gels.• Highly Concentrated: Elute in as little as 6 µl.• Ultra-Pure: Ready for Next-Gen Sequencing, PCR, ligations, etc.

Clean & Concentrated DNA. DNA samples, such as the PCR products shown here, can be efficiently purified and concentrated using the DNA Clean & Concentrator®-5.

Marker

PCR

Product

Primers

600

300

200

100

Original PCR Purified PCR

DNA fragments recovered from an agarose gel using the Zymoclean™ Gel DNA Recovery Kit. Lanes: M: DNA Ladder; 1-5: individual ladder DNA fragments.

23 kb9 kb

2 kb

500 bp

M 1 2 3 4 5

DNA sequencing chromatogram of a PCR product recovered using the Zymoclean™ Gel DNA Recovery Kit. DNA was recovered from a 2% (w/v) agarose gel and used directly for sequencing.

6 µl elution volume, 2 minute procedure, 0 µl retention volume

Product Cat. No. Size

DNA Clean & Concentrator® -5 D4013 50 preps D4003S (10 preps)

Zymoclean™ Gel DNA Recovery Kit D4007 50 preps D4001S (10 preps)

SAMPLES AVAILABLE

DN

A

10

DNA: Plasmid DNA Purification

ZymoPURE™ Technology Overview

ZymoPURE™ Kit Comparison

Kit Processing Time Culture Volume Elution Volume Plasmid Yield Endotoxins

Miniprep ≤ 15 minutes ≤ 5 ml ≥ 25 µl 100 µg ≤ 1 EU/µg

Midiprep ≤ 20 minutes 50 ml ≥ 100 µl 300 µg ≤ 0.025 EU/µg

Maxiprep ≤ 20 minutes 150 ml ≥ 200 µl 1.2 mg ≤ 0.025 EU/µg

Gigaprep ≤ 50 minutes 2.5 L ≥ 2 ml 10 mg ≤ 0.025 EU/µg

Our EZ-Load™ technology replaces slow gravity flow columns with rapid binding and washing using a vacuum or centrifuge. The EZ-Elute™ system makes time-consuming alcohol precipitation steps obsolete by enabling elution of highly concentrated plasmid DNA from a spin-column using a microcentrifuge. The EndoZero™ Spin-Column removes endotoxins with a simple 1 minute spin.

Simple 20 minute EndoZero™ Midi/Maxi preps

ZymoPURE™ II Save > 1 hour

Protocol Length (min.)

Supplier Q

Supplier MN

30 90 15060 1200

rapid loading onto a spin-column via vacuum or centrifuge

bindfor ultra-pure endotoxin free plasmid DNA

washtransfection ready from spin-column

elute

DN

A

11

Supplier Q1 Supplier Q2ZymoPURE™ IIM

ZymoPURE™ Plasmid Kits (Mini, Midi, Maxi, Giga)

• Fastest: Simple 20 minute Midi/Maxipreps.• Highest Yield: 6x more plasmid.• Ultra-Pure: EndoZero™, vaccine grade*, and transfection ready.

Product Cat. No. Size

ZymoPURE™ Plasmid Miniprep Kit D4209 50 preps D4208S (10 preps)

ZymoPURE™ II Plasmid Midiprep Kit D4200 25 preps D4200S (2 preps)

ZymoPURE™ II Plasmid Maxiprep Kit D4202 10 preps D4202S (2 preps)

ZymoPURE™ II Plasmid Gigaprep Kit D4204 5 preps –

SAMPLES AVAILABLE

Ultra-pure Vaccine Grade Plasmid DNA

Highest Yield & Lowest Elution Volume

The EndoZero™ spin column removes exdotoxins with a simple spin

Endotoxins below FDA Limit for VaccinesFDA Limit for Vaccines < 0.04 EU/µg DNA

Supplier QEndoFreeZymoPURE

™ II

End

oto

xin

Uni

ts/µ

g P

lasm

id D

NA

Supplier Q

0.000

0.025

0.050

0.075

0.100

2

4

6

8

10

Stated manufacturers’ endotoxin levels for the ZymoPURE™ II Maxiprep kit compared to two separate kits from Supplier Q.

* Endotoxins

12

E. c

oli

Product Cat. No. Size

Mix & Go!™ Competent Cells - DH5 Alpha (10 x 100 μl) T3007 10 x 100 µl aliquots (10 tubes) T3007S (4 tubes)

Mix & Go!™ Competent Cells - DH5 Alpha (96 x 50 μl) T3009 96 x 50 µl aliquots (12 x 8-tube strips) –

E. coli: Competent Cells

Mix & Go!™ Competent Cells (DH5 Alpha)

• Simple 20 Second Transformation: No heat shock! Just add DNA and spread. • High Transformation Efficiencies: Achieve 108 – 109 transformants per µg of plasmid DNA.• Versatile: Excellent for general cloning, blue-white screening, and plasmid isolation.

SAMPLES AVAILABLE

Mix & Go!™

Competent Cells Others

42°C 2 min. (Heatshock)

Place on ice

Spin to concentrate cells

Remove Supernatant

Add SOC

for 20 second* transformations!

Ice 45 min.

*Ampicillin selection only

Simple 20 second Transformation

13

Microbiomics: Microbial Analysis

ZymoBIOMICS® Microbial Community Standard

• Microbiome Standard: Mock microbial community of well-defined composition.• Identify Bias: Contains both tough-to-lyse and easy-to-lyse organisms.• Accurate Characterization: Ideal for validation, optimization, and quality control of complete microbiome workflows.

• Microbiome DNA Standard: Eight bacteria and two yeast genomes.• Identify Bias in Library Prep Methods: The DNA has a wide GC range of 15% - 85%. • Accurate Composition: Ideal for validation, optimization, and quality control of microbiome workflows.

ZymoBIOMICS® Microbial Community DNA Standard

DNA from three Gram-negative bacteria, five Gram-positive bacteria, and two tough-to-lyse yeasts. The ZymoBIOMICS® Microbial Community Standards are perfect for assessing bias in popular extraction methods. The microbial standards are accurately characterized, with a wide GC range (15% – 85%) and contain negligible impurities (< 0.01%), enabling easy exposure of artifacts, errors, and bias in microbiomics or metagenomic workflows.

Species Avg. GC (%) Gram StaingDNA Abun.

(%)

Pseudomonas aeruginosa 66.2 - 12

Escherichia coli 56.8 - 12

Salmonella enterica 52.2 - 12

Lactobacillus fermentum 52.8 + 12

Enterococcus faecalis 37.5 + 12

Staphylococcus aureus 32.7 + 12

Listeria monocytogenes 38.0 + 12

Bacillus subtilis 43.8 + 12

Saccharomyces cerevisiae 38.4 Yeast 2

Cryptococcus neoformans 48.2 Yeast 2

Product Cat. No. Size

ZymoBIOMICS® Microbial Community Standard D6300 10 preps

ZymoBIOMICS® Microbial Community DNA Standard D6306 2,000 ng

Theoretical

0%

40%

20%

60%

80%

100%

DNA Standard

Micro

bio

mics

14

Quick-DNA™ Fecal/Soil Microbe Miniprep Kit

• Boost Your Detection: Included BashingBeads™ ensure complete lysis of tough-to-lyse samples.• Inhibitor-Free: Ready for qPCR, Next-Gen Sequencing, arrays, etc. • Simple Workflow: Lyse, purify on column, and filter to remove PCR inhibitors.

• Microbiomics-grade DNA Extraction: Unbiased cellular lysis for accurate microbiome measurements and certified low bioburden.• Ultra-pure: Inhibitor-free DNA from any sample that is ready for qPCR, NGS, etc.• Simple 20 Minute Workflow: No precipitations or lengthy incubations.

ZymoBIOMICS® DNA Miniprep Kit

The ZymoBIOMICS® DNA Miniprep Kit provides accurate representation of the organisms extracted from the

ZymoBIOMICS® Microbial Community Standard.

Product Cat. No. Size

ZymoBIOMICS® DNA Miniprep Kit D4300 50 preps D4300S (5 preps)

Quick-DNA™ Fecal/Soil Microbe Miniprep Kit D6010 50 preps –

SAMPLES AVAILABLE

Bacillus subtilis (G+)

Listeria monocytogenes (G+)

Staphylococcus aureus (G+)

Enterococcus faecalis (G+)

Lactobacillus fermentum (G+)

Salmonella enterica (G-)

Escherichia coli (G-)

Pseudomonas aeruginosa (G-)

ZymoBIOMICS®

Unbiased Biased

Mic

rob

ial C

om

po

sitio

n (1

6S C

oun

ts)

Theoretical

0%

40%

20%

60%

80%

100%

HMP Protocol Supplier M Supplier Q

High-quality total DNA was isolated from different environmental sample sources using the Quick-DNA™ Fecal/Soil Microbe Kit and compared against other suppliers. (A) Equivalent amounts of feces were processed using each kit, then equal volumes of eluted DNA were analyzed on a 0.8% (w/v) agarose gel stained with EtBr. (B) Metagenomic DNA isolated from 5 soil samples. M: 1 kb marker (NEB); 1-5: soil samples (sand, sandy clay loam, hydrophobic sandy loam course, sandy loam, fine gravel).

Higher YieldsM 1 2 3 4 5

Supplier A Supplier B

Quick-DNA™ Fecal/Soil

Microbe Kit

Mic

rob

iom

ics

Microbiomics: Microbial Analysis

15

Micro

bio

mics

1.5 hourhands-on time

4 hourshands-on time

• Fast & Simple: Only 1.5 hours of hands-on time. No TapeStation® analyses or AMPure® clean-ups.• Accurate: Real-time PCR reduces PCR chimera formation by up to 10 times.• Increased Coverage: Novel primers increase phylogenetic coverage of bacteria and archaea, enabling species-level resolution for

human microbiome profiling.

The Quick-16S™ NGS Library Prep Kit minimizes PCR chimera formation compared to two common protocols: Human Microbiome Project (HMP) and Earth Microbiome Project (EMP). Equivalent amounts of the same fecal DNA sample were used as input. Chimeric sequences were predicted with Uchime (https://www.drive5.com/uchime).

The Quick-16S™ NGS Library Prep Kit is >2.5 times faster than the conventional 16S library prep method. The Quick-16S™ Kit simplifies the 16S library prep workflow by quantifying libraries using qPCR, instead of TapeStation® analyses, and by using a single-tube library cleanup.

A. The Quick-16S™ Primer Set V1-V2 includes coverage of common human-associated microbes, including Bifidobacterium, Propionibacterium, and Chlamydia, which are missed in common V1-V2 or V1-V3 primers.B. The Quick-16S™ Primer Set V3-V4 provides up to 87% coverage for archaea, organisms commonly found in the human gut. The common V3-V4 primers provide 0% coverage for archaea.

Minimize PCR Chimera Formation Best Phylogenetic Coverage

0

Quick-16S™

NGS Library Prep Kit

Chi

mer

ic S

eque

nces

(%)

HMP Protocol (30 cycles)

EMP Protocol (35 cycles)

2

4

6

8

10

12

Fastest 16S Library Prep

0Archaea

Quick-16S™ Primer Set V1-V2HMP V1-V3 PrimersLoman Lab V1-V3 Primers

Bacteria

Co

vera

ge

(%)

20

40

60

80

100

A.0

Archaea

Quick-16S™ Primer Set V3-V4Klindworth V3-V4 Primer Set

Bacteria

Co

vera

ge

(%)

20

40

60

80

100

B.

Quick-16S™ NGS Library Prep Kit

Product Cat. No. Size

Quick-16S™ NGS Library Prep Kit D6400 96 rxns

16

ChIP DNA Clean & Concentrator® Kit

• Fast: Two-minute DNA clean-up from any step in a standard ChIP protocol. • High-Quality: Ultra-pure, concentrated ChIP DNA can be eluted in as little as 6 μl.• Ready to Use: DNA is ideal for PCR, arrays, DNA quantification, Southern blot analysis, sequencing, and other molecular

applications.

Epig

enet

ics

Product Cat. No. Size

EZ DNA Methylation-Lightning® Kit D5030 50 rxns D5030S (10 rxns)

ChIP DNA Clean & Concentrator® (capped columns) D5205 50 preps –

Epigenetics

EZ DNA Methylation-Lightning® Kit

• Streamlined Process: Ready-to-use conversion reagent is added directly to DNA. Purified bisulfite converted DNA in < 1.5 hours.• High-Quality: Bisulfite-converted DNA has > 99.5% conversion efficiency with reduced fragmentation.• NGS-Ready: Low DNA input requirement makes it ideal for preparing whole genome or targeted enrichment bisulfite libraries for

methylation analysis.

SAMPLES AVAILABLE

The EZ DNA Methylation-Lightning® Kit exhibits more efficient amplification resulting in earlier quantification cycles (Cg) than the comparable kit from Supplier Q.

10000

7000

4000

1000

34 39 44

-2000

RFU

(103

)

MGMT

Cycles

Supplier Q

NTC

1-2 Cycles

EZ DNA Methylation-Lightning® Kit

29

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SAMPLES AVAILABLE

www.zymoresearch.com Toll Free: (888) 882-9682info@zymoresearch.com

Cat. No. B1011 Version 05

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