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bacteria characteristics, morphology, culture media, History, clinical manifestation
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GROUP 2Capnocytophaga canimorsus
Spingomonas paucimobilisMoraxella spp.
Moraxella nonliquefaciensMoraxella lacunataMoraxella osloensis
CapnocytophagaGENERAL CHARACTERISTICS:Gram-negativeFacultative anaerobePleomorphicCapnophilicColor of colonies- pink to yellowishNon-hemolytic except: C.haemolytica
CapnocytophagaPATHOGENICITY:Oral diseases & brain abscessOro pharynx (periodontal diseases, opthalmic
lesions, RT infections, traumatic pericarditis, mediastinal or cervical abscesses & peritonitis)
Capnocytophaga5 NORMAL INHABITANTS OF ORAL
CAVITY:C. ochraceaC. gingivalisC. sputigenaC. haemolyticus andC. granulose *** Oxidase & Catalase (-)
Capnocytophaga canimorsusNormal inhabitants of the oral cavity of dogs
and cats Oxidase and Catalase (+) Penicillin is the drug of choice
SpingobacteriumGram negativeNon-fermentative bacilliRarely involved in human infectionsFlavobacterium regarded as members of the
genus at one timeSensitive to SXT and FluoroquinolonesResistant to amino glycosides, Clindamycin
and Polymyxin B.Susceptibility to B-lactame antibiotics is
variable
Spingobacterium6 Species:-S. spiritovorum-S. multivorum-S. mizutae-S. antarcticum-S. faecium-S. thalpophilum
Spingobacterium
2 most frequently isolated species: S.multivorum
-isolated from blood of patients (septicemia & peritonitis)
S. spiritovorum-isolated from clinical specimens (urine,blood & hospital environments)
***Very similar biochemically.
SpingobacteriumS.spiritovorum
-produces mannitol, ethanol and rhamnose (S.mutivorum)
Both produces yellow pigmentBoth were identified & processed for:
DNaseOxidaseUrease activityMotility test
Gram negative (-), rod-shapedNon-spore formingStrictly aerobic ChemoheterotrophicUbiquinone 1036 different species2 clinically signifant: S. paucimobilis & S.
parapaucimobilis
Spingomonas
S. paucimobilis-isolated environmentally from water, including swimming pools, hospital equipment & laboratory supplies.-causative agent of peritonitis associated with chronic ambulatory peritoneal dialysis, septicemia, meningitis, leg ulcer, empyema and splenic and brain abscesses
Spingomonas
Spingomonas
Biochemical charcteristics: yellow pigment on MAK agar (48hrs. for
growth on SBA) Isolates: Weakly oxidase (+) (Some strains may be (-) Motile at 18oC-22oC but not 37oC. Urease (-) Indole (-) Oxidizers
Resistant to antimicrobial agentsSusceptible to :
AminoglycosidesTetracyclinesChloramphenicol SXTPolymyxin B
*3rd generation Cephalosporins and Fluoroquinolones
Spingomonas
SpingomonasS. parapaucimobilis isolated from sputum, urine and vaginal
specimens Isolates have been found:EsterasesEndotoxinLipasesPhosphatasesS. parapaucimobilis resembles S.
paucimobilisExcept isolates of S. parapaucimobilis:
H2S (+) by the lead acetate methodSimon citrate (+)DNase (-)
Moraxella Family Moraxellaceae named after the Swiss ophthalmologist
Victor Morax Coccobacillary to bacillary gram-negative
bacilli Non-motile, but some has a “twitching”
mobility Encapsulated with pili 13 known Moraxella spp.
Moraxella
Biochemical characteristics: Strictly aerobic Strongly Oxidase (+) Beta-lactamase, Catalase and Dnase (+) Biochemically inert to carbohydrate
oxidation Susceptible to Penicillin
MORAXELLA
Cultural characteristics: Smooth, opaque, gray to white, colony may
be swept intact- “hockey pockey” 48 hour colony- “Wagon wheel” Grow on Nutrient Agar at 22◦C Failure to grow in TMA (Thayer Martin Agar) Reduces nitrate to nitrite Assacharolytic, tributyrin hydrolysis
POSITIVE Non-hemolytic on BAP
MoraxellaEpidemiology: Conjunctivitis Host range: Humans and mammals
Mode of Transmission: Person-to-person especially in hospitals Environmental contamination Aerosol transmission
MoraxellaHabitat: Mucous membranes of human & lower animals Isolated from:
- respiratory tract- urinary tract (genitourethral tract)- eye- inflammatory secretions of the middle ear- maxillary sinus- bronchial aspirate- nasal cavity- spleen- cerebrospinal fluid- joints- bursa
MoraxellaPathogenecity/Toxicity:o Moraxella catarrhalis
- acute otitis media- chronic & serious otitis media- acute & chronic sinusitis- upper and lower respiratory tract infections- systemic infections- meningitis - bacteremia- endocarditis- keratitis- suppurative arthritis
Moraxellao Moraxella nonliquefaciens
- upper & lower respiratory tract infections
o Moraxella lacunata- endocarditis- conjunctivitis- otolaryngologic infections - keratitis
Moraxella
o Moraxella osloensis- osteomyelitis- meningitis- arthritis
MoraxellaDRUG SUSCEPTIBILITY All are susceptible to Penicillin except
M.catarrhalis. M.catarrhalis
resistant to:-Penicillin-Ampicillin-Amoxicillin-Vancomycin-Clindamycin
Susceptible to Amoxicillin,Tetracyclines, Rifampin, and Erythromycin
MORAXELLA
SUSCEPTIBILITY TO DISINFECTANTS: Susceptible to 2-5% phenol 1% sodium hypochlorite 4% formaldehyde 2% glutaraldehyde 70% ethanol 70% propanol 2% peracetic acid 3-6% hydrogen peroxide Iodine
SURVIVAL OUTSIDE HOST:Moraxella bovis -survive up to 3 days on
insect legsM. catarrhalis - survive in expectorated
sputum for at least 3 weeksMoraxella nonliquefaciensSecond most commonly isolated member of
the genus after M.catarrhalis.Normal flora of respiratory tract; rarely cause
disease in humanCan survive up to 40 weeks (-70 & -80°C)
Moraxella
MoraxellaCultural characteristics: Smooth, translucent to semi-opaque;
occasionally, colonies spread & pit agar in 5%BA Non-lactose fermented in MAC
Biochemical characteristics: Catalase (+) DNase, Urease and Gelatin hydrolysis (-) Phenylalanine deaminase (PNA) negative Digests Loeffler’s Slant (-);Sodium Acetate
Utilization (-) Growth supported by Nutrient Agar
MORAXELLAPathogenecity:
Bacteremia Keratitis Endophthalmitis
o Moraxella osloensis-grows and produces an alkaline reaction in acetate medium-normal flora in genitourinary tract-Urease (+)-PDA (+)-a systemic serious disease
Moraxella
PATHOGENECITY:-endocarditis-meningitis-osteomyelitis-septic arthritis-vaginitis -bacteremia
Moraxella
o Moraxella lacunata Common conjunctival isolate small coccobacillus causative agent of
“blepharoconjunctivitis” Gelatin (+) Urease (-) Unable to grow on MAC agar PDA (+)
SPECIES MACAgar
BAPAgar
Catalase Dnase Urease
Capnocytophaga
-+ + -+ - -
Spingomonas + + + - -
M.nonliquefaciens
- + + - -
M.osloensis - + - - +
M.lacunata - + - - -
SUMMARY TABLE
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-Ana-Dayle
-Kristele-Mollie