Molecular Weight Markers

・DNA Step Ladders

・OneSTEP Ladders

・Ladders for 10minute separation

・Wide Range Ladders

・Markers ＆ OneSTEP Markers

・DGGE Markers

・PCR Marker Makers

・Easy SeparatorTM

・Negative Gel Stain MS Kit

・SuperSepTM Ace

・SuperSepTM DNA

・RNA Size Standard Markers

・RNA Step Ladder

・Loading Buffers

RNA Si St d

・Prestain markers

・ Western marker

・ Biotinylated marker

・ Markers for CBB dyeing

co.jp

・PCR Marker Makers

・Easy SeparatorTM

・Loading Buffers

Molecular-WeightMarkers

DNA Ladder

10 bp DNA StepLadder (10-100 bp)




DNA Step Ladder(10-300 bp)

100 bp DNA Step Ladder(0.1-1.5 kbp)

OneSTEP Ladder 50(0.05-2 kbp)

OneSTEP Ladder 100(0.1-2 kbp)

DNA Step Ladder (1~6)for low-molecular-range analysis.

Loading:1µL/lane

Loading:5-10µL/lane

Loading:5µL/lane

Concentration:0.69µg/5µL

Concentration:0.755µg/5µL

Loading:5µL/lane

1% Agarose

5% Agarose 21

3% Agarose 21

2% Agarose

3% Agarose 21

2% Agarose

3.5% Agarose

　　

2% Agarose

1% Agarose S

1% Agarose S

1% Agarose S

2% Agarose S

1

5 6 7 8

2 3 4

9 10 11 12

OneSTEP Ladder 500 (0.5-5 kbp)

OneSTEP Ladder 1 kb(1-10 kbp)

OneSTEP LadderSupercoiled Plasmid

Gene Ladder 100(0.1-2 kbp)

Loading:5µL/lane

Loading:5µL/lane

100

（bp）

（bp）（bp）（bp）（ng/5µL）

908070605040302010

（bp）（ng）500 60

（bp）

（bp）（kbp）（kbp）

800400 60 300 60 250 60 200 120 175 80 150 80 125 80 100 120 75 80 50 80 25 80

700600500400300

150200

10050

1,000900

1,500

800

717600500400

286

200

100

2,0001,8001,6001,4001,2001,000900800700600500400300200100

10090807060100454035305020151010

30020015010075503525201510

5,0004,500

3,5003,0002,5002,000

1,500

1,000

4,000

500

10.09.08.07.06.05.04.03.0

2.0

1.0 100

500

200

（bp）（ng/5µL）

300400

6007008009001,000

1,5002,000

100

75

203040

3035404550

75100

10.09.08.0

6.05.0

4.0

3.0

7.0

OneSTEP Ladder (7~12)for simultaneous measurement of DNA size and amount.

each fragment: 50ng/5µL



Concentration: 0.64µg/5µL

DN

A La

dder

DN

A La

dder

Readyto

use

With dye and glycerol already added, it is ready to be applied to the agarose gel.

6xBlue/OrangeLoading

Dye

6xBlue/OrangeLoading

Dye

－20℃ －20℃ －20℃ －20℃

Readyto

use

Readyto

use

Readyto

use

Readyto

use

Readyto

use

Readyto

use

－20℃

－20℃

Readyto

use

－20℃

300275250225200175150125100755025

（bp）

（bp）（ng/5µL）2,0001,5001,000900800700600500400450350300250200150100

50

1007550454035307522.52017.51537.5107.510

100

6xLoading

Dye

6xLoading

Dye

6xLoading

Dye

2

1% Agarose S

1% Agarose S

2% Agarose

4% Agarose 21

2% Agarose

2% Agarose

13 14 15 16

17

18

DNA Step Ladder(10-200 bp)

DNA Step Ladder(100-5,000 bp)

DNA Step Ladder(50-1,500 bp)

DNA Step Ladder(500-10,000bp)

Gene Ladder Fast 1(4 bands:0.1-2 kbp)

Gene Ladder Fast 2(6 bands:0.1-10 kbp)

（bp）（bp）（bp）（bp）（ng/5µL）

（bp）（ng/5µL）

200100

50

20

2,000 251,000 25500 25100 25

50

200

400

850

1,500

10

100

400

850

2,000

（bp）

5,00010,0004,0002,000

1,000

500

10,000 254,000

252,000

25

1,00025

500 25100 25

Loading:20µL/lane

Loading:20µL/lane

Loading:20µL/lane

Loading:20µL/lane

Ladder for 10minute separation (13~18)

123456

7

8

9

10

1112131415161718

10 bp DNA Step Ladder (10-100 bp) 25 bp DNA Step Ladder (25-300 bp) 25 bp DNA Step Ladder (25-500 bp) 50 bp DNA Step Ladder (50-800 bp) DNA Step Ladder （10-300 bp） 100 bp DNA Step Ladder （0.1-1.5 kbp）

OneSTEP Ladder 50 （0.05-2.0 kbp）

OneSTEP Ladder 100 （0.1-2 kbp）

OneSTEP Ladder 500 (0.5-5 kbp)

OneSTEP Ladder 1 kb （1-10 kbp）

OneSTEP Ladder Supercoiled PlasmidGene Ladder 100 （0.1-2 kbp）DNA Step Ladder （10-200 bp）, Fast Separation TypeDNA Step Ladder （50-1,500 bp）, Fast Separation TypeDNA Step Ladder （100-5,000 bp）, Fast Separation TypeDNA Step Ladder （500-10,000 bp）, Fast Separation TypeGene Ladder Fast 1（4 bands:0.1-2 kbp）Gene Ladder Fast 2 （6 bands:0.1-10 kbp）

040-28721292-69001044-30711544-02311298-66301296-68801319-06044315-06041311-06043317-05244313-05241319-05243317-05364313-05361319-05363314-05374310-05371316-05373317-05381316-06951292-66201290-61001296-61101292-61201317-06981314-06991

32.5 μg (50 μL) 100 μg 500 μL 85 μg (250 μL) 50 μg 30 μg 25 μL500 μL500 μL ×2 25 μL500 μL500 μL ×2 25 μL500 μL500 μL × 3 25 μL500 μL500 μL × 3500 μL500 μL10 μg （0.5mL） × 210 μg （0.5mL） × 210 μg （0.5mL） × 210 μg （0.5mL） × 2500 μＬ × 2500 μＬ × 2

Code No.Number Product name Package Size Note

: Nippon Gene’s products

Store at ｰ20℃Store at 2～10℃

　　　　　　:

NO DISPLAY:

－20℃



Readyto

use

－20℃

Readyto

use

－20℃

Readyto

use

－20℃

Readyto

use

Readyto

use

Readyto

use

－20℃

6xLoading

Dye

6xLoading

Dye

6xLoading

Dye

6xLoading

Dye

3

DNA Step Ladder Mix(0.08-10 kbp)

Smart Ladder(0.2-10 kbp)

Gene Ladder Wide 1(0.1-20 kbp)

Gene Ladder Wide 2(0.1-20 kbp)

Marker 1

(λ/Hind Ⅲ)

Marker 2

(λ/Hind Ⅲ・EcoRⅠ)

Marker 3

(λ/Hind Ⅲ+λ/EcoRⅠ)

Marker 4

(φX174/Hae Ⅲ)

3% Agarose 21

1% Agarose S

1% Agarose S

1% Agarose S

1% Agarose S

1% Agarose

1% Agarose

3% Agarose 21

1% Agaose S

0.3% Agarose H

0.3% Agarose H

Marker 5

(φX174/HincⅡ)

Marker 6

(λ/StyⅠ)

Marker 7GT

(T4 GT7+T4 GT7/BglⅠ)

Marker 8GT

(T4 GT7+T4 GT7/BglⅡ)

20,00010,0007,000

600500400200100

5,0004,0003,000

2,0001,5001,000900800700

100 50 35

30 75 40 20100

75 40 30

100 30 504540 35

（ng/20µL）（bp）

（kbp）（kbp）（kbp）（bp）

（ng/5µL）（bp）（ng/5µL）（bp）（ng/5µL）（bp）

（bp）（kbp）（kbp）（kbp）

10,0008,0006,0005,0004,000

10,0008,0006,0005,0004,0003,0002,5002,0001,5001,000800600400200

100806050403025201510080604020

20016012010080

3,000 602,500 502,000 40

1,500 32

1,030 200900 180800 160700 140600 120500 200400 80300 60200 40100 2080 16

20,00010,0007,0005,0004,0003,000

2,0001,500

1,0001,300

700500400300200100

1005035754030

10030

10026

3575403020100

1% Agarose

19 20 21 22

27 28 29 30

23 24 25 26

23.13 21.23

5.15

4.98

4.27

3.52

2.02

1.91

1.58

1.38

0.95

0.83

23.13 1,353

31028127123419411872

21.239.427.426.565.815.654.884.363.522.322.02

9.426.56

4.36

2.322.02

1,078872603

1,057 19.33 165.65 165.6556.74

17.8217.7015.7114.2810.65

8.03

5.595.51

50.3141.77

23.4521.1017.70

10.06

7.746.22

4.263.47

2.69

1.88

1.49

0.93

770612495392345341335297291210162

79

DNA molecular-weight markers that set size standards for linear double-strand DNA. Each One-Step Marker comes with pre-added dye and glycerol, so they can be applied directly to the agarose gel.

Wide Range Ladder (19~22)for wide-range and simultaneous analysis of both DNA size and amount.

DNA molec lar eight markers that set si e standards for linear do ble strand DNA E

Marker & the OneSTEP Marker (23~33)

der (19~22)l i f b th DNA i d tl i f b th

DNA Ladder

DN

A La

dder

－20℃

－20℃

Readyto

use

Readyto

use

Readyto

use

6xLoading

Dye

Readyto

use

－20℃

4

3% Agarose 21

3% Agarose 21

3% Agarose 21

Marker 9

(φX174/HinfⅠ)

Marker 10

(pBR322/MspⅠ)

Marker 11

(pUC19/MspⅠ)

（bp）（bp）（bp）

31 32 33

72671355350042741741331124920015114011810082666648

622527404307242238217201190180160160147147123110907667

501489404331242190147111110

23

24

25

26

27

28

29

30

31

32

33

19

20

2122

DNA Step Ladder Mix （0.08-10 kbp）

Smart Ladder (0.2-10 kbp)

Gene Ladder Wide 1 （0.1-20 kbp）Gene Ladder Wide 2 （0.1-20 kbp）

OneSTEP Marker 1 （lambda/Hind Ⅲ digest）

Marker 1（lambda/Hind Ⅲ digest）

OneSTEP Marker 2 （lambda/Hind Ⅲ + EcoR I double digest）

Marker 2（lambda/Hind Ⅲ + EcoR I double digest）

OneSTEP Marker 3 （lambda/Hind Ⅲ + lambda/EcoR I digest mixture）

Marker 3（lambda/Hind Ⅲ + lambda/EcoR I digest mixture）

OneSTEP Marker 4 （phiX174/Hae Ⅲ digest）

Marker 4 （phiX174/Hae Ⅲ digest）

OneSTEP Marker 5 （phiX174/Hinc Ⅱ digest）

Marker 5 （phiX174/Hinc Ⅱ digest）

OneSTEP Marker 6 （lambda/Sty Ⅰ digest）

Marker 6（lambda/Sty Ⅰ digest）

Marker 7 GT

Marker 8 GT

OneSTEP Marker 9 （phiX174/Hinf Ⅰ digest）

Marker 9 （phiX174/Hinf Ⅰ digest）

OneSTEP Marker 10 （pBR322/Msp Ⅰ digest）

Marker 10 （pBR322/Msp Ⅰ digest）

OneSTEP Marker 11 （pUC19/Msp Ⅰ digest）

Marker 11 （pUC19/Msp Ⅰ digest）

292-68401313-03943317-03941313-06961310-06971310-05251314-05254316-00454312-00456317-05261311-05264319-00564315-00566314-05271310-05273316-00574312-00576318-05791314-05793315-00664311-00666311-05801317-05803312-00674318-00676311-05281317-05283313-00964319-00966315-03981311-03983312-03991318-03993318-05811314-05813314-01491310-01493315-05821311-05823317-01501313-01503312-05831318-05833319-03261315-03263

1 mL25 μL500 μL500 μL × 2500 μL × 21500 μL1500 μL × 5 120 μg 120 μg × 51500 μL1500 μL × 5 80 μg80 μg × 5

1500 μL1500 μL × 580 μg80 μg × 5 375 μL375 μL × 515 μg15 μg × 5375 μL375 μL × 515 μg15 μg × 5

1500 μL1500 μL × 580 μg80 μg × 5 10 μg10 μg × 510 μg10 μg × 5375 μL375 μL × 515 μg15 μg × 5375 μL375 μL × 515 μg15 μg × 5375 μL375 μL × 515 μg15 μg × 5


Storage conditionsDNA MW Marker ：　

OneSTEP Marker：　Store at 2～10℃

－20℃


5

DGGE MarkerⅠ(5 fragments)

DGGE Marker Ⅱ(10 fragments)

DGGE Marker Ⅲ(10 fragments)

DGGE Marker Ⅳ(8 fragments)

DGGE Marker Ⅴ(9 fragments)

8% Acrylamide

8% Acrylamide

6% Acrylamide

6% Acrylamide

7% Acrylamide

No. of fragments (Conc.)Application

Constant voltageElectrophoresis time, Temp.

5 (50ng/5µL)For bacterial 16S rDNA

10 (100ng/5µL) For bacterial 16S rDNA

10 (100ng/5µL)For bacterial 16S rDNA

8 (80ng/5µL)For fungal bacteria16S rDNA

9 (50ng/5µL)For fungal bacteria 16S rDNA

120V8 hours, 60℃

120V8 hours, 60℃

50V18 hours, 58℃

50V20 hours, 60℃

75V16 hours, 60℃

3

5

1

2

4

Denaturant 35%

65%

Denaturant 35%

65%

Denaturant 50%

70%

Denaturant 20%

Denaturant 20%

45% 50%35 36 37 38

12345678

12345

78910

6

1

2

345

8

67

9

（bp）（bp）105777061249539234534133529729121016279

1005905800702601502398299200101

DNA markers for DGGE (denaturing gradient gel electrophoresis). A CG clamp is added at the end of one side of each fragment to enable separation / inspection by DGGE.

12

43

5678910

M：Marker 5 （φX174/Hinc Ⅱ）39：100L （101 bp, 200 bp, 299 bp, 398 bp, 502 bp）40：100H （601 bp, 702 bp, 800 bp, 906 bp, 1,005 bp）41：200U （101 bp, 299 bp, 502 bp, 702 bp, 906 bp）42：200E （200bp, 398 bp, 601 bp, 805 bp, 1,005 bp）43：Mixture of products from a 100L & 100H PCR

0.5 µg3 µL3 µL3 µL3 µL

3 µL each

DNA markers for DGGE (dena

DGGE Marker (34~38)

for preparing DNA size markers. Consisting of a mixture of template DNA, primer, and reaction buffer, the reagent initiates a PCR with the simple addition of a Tag DNA polymerase “Gene Tag.” It can also be used as a positive control while the PCR is under way.

for preparing DNA size markers Consisting of a mixt

PCR Marker Maker (39~42)

aturing gradient gel electrophoresis) A CG clamp is aaturing grad

4~38)

DNA Ladder

DN

A La

dder

34

－20℃ －20℃ －20℃ －20℃ －20℃

－20℃

34

35

36

37

38

39

40

41

42

DGGE Marker Ⅰ (5 fragments)

DGGE Marker Ⅱ (10 fragments)

DGGE Marker Ⅲ (10 fragments)

DGGE Marker Ⅳ (8 fragments)

DGGE Marker Ⅴ (9 fragments)

PCR Marker Maker 100L PCR Marker Maker 100L, Gene Taq attachedPCR Marker Maker 100HPCR Marker Maker 100H, Gene Taq attachedPCR Marker Maker 200U PCR Marker Maker 200U, Gene Taq attachedPCR Marker Maker 200E PCR Marker Maker 200E, Gene Taq attached

318-06411314-06413312-06414311-06401317-06403315-06404315-06921311-06923319-06924312-06931318-06933316-06934319-06941315-06943313-06944317-03061314-03071311-03081318-03091318-03111315-03121312-03131319-03141

30 μL （for 6 applications）150 μL （for 30 applications）150 μL × 5 （for 150 applications）30 μL （for 6 applications）150 μL （for 30 applications）150 μL × 5 （for 150 applications）30 μL （for 6 applications）150 μL （for 30 applications）150 μL × 5 （for 150 applications）30 μL （for 6 applications）150 μL （for 30 applications）150 μL × 5 （for 150 applications）30 μL （for 6 applications）150 μL （for 30 applications）150 μL × 5 （for 150 applications）For 10 applicationsFor 10 applicationsFor 10 applicationsFor 10 applicationsFor 10 applicationsFor 10 applicationsFor 10 applicationsFor 10 applications


39 40 41 42 43M

: Nippon Gene’s products6

aa b c d e f b c d e f

Xylene Cyanol FF

Bromophenol Blue

Orange G

1% formaldehyde Agarose

5% polyacrylamide-urea



RNA Size Standard

Marker ⅡRNA Size Standard

Marker Ⅲ (0.1-1 kb)

RNA Size Standard

Marker Ⅳ (0.28-6.58 kb)

RNA Ladder(0.125-6 kb)

RNA Ladder

1 2 3 4

9

750500400

300

200

100

（k base）（base）

1,000

（base）

6,583

（base）

6,0004,0003,0002,0001,5001,000500250125

4,9813,638

2,604

1,908

1,383

623

281

955

654

32.52

1.5

1

0.5

RNA Size Standard Marker (1~4) RNA molecular-weight markers prepared by in vitro transcription.

RN

A La

dder

－80℃ －70℃－70℃－70℃

2% Native Agarose

2% Formaldehyde Agarose

A sample can be easily applied during DNA gel electrophoresis to effectively predict the migration point.

Loading Buffer

1 % agarose S2 % agarose S3 % agarose 21

100 bp (approx.) 30 bp (approx.) 10 bp (approx.)

900 bp (approx.)300 bp (approx.)100 bp (approx.)

5,000 bp (approx.)1,500 bp (approx.) 500 bp (approx.)

Orange G Bromophenol Blue Xylene Cyanol FF

Marker 4(φX173/Hae Ⅲ)Product PCR(299 bp) 50 ng

TE Buffer

6 × Loading Buffer Triple Dye6 × Loading Buffer Orange G6 × Loading Buffer Triple Dye6 × Loading Buffer Orange G6 × Loading Buffer Triple Dye6 × Loading Buffer Orange G

abcdef

100

200

300400

6008001,000

100

200

300400

6008001,000

（base）（base）

RNA Step Ladder (0.1-1 kb)

5

123

4

5－－－－

RNA Size Standard Marker Ⅱ （0.5-9 kb） RNA Size Standard Marker Ⅲ （0.1-1 kb）RNA Size Standard Marker Ⅳ （0.28-6.58 kb）

RNA Ladder （0.125-6 kb）

RNA Step Ladder （0.1-1 kb）Loading Buffer6 × Loading Buffer OrangeG6 × Loading Buffer Double Dye6 × Loading Buffer Triple Dye

542-00651545-01621188-01831311-06261317-06263298-61301313-90111317-90251313-90351314-90261

50 μg50 μg50 μL25 μL25 μL × 240 μL × 510 mL1 mL × 31 mL × 31 mL × 3



（Store at room temp.）

－80℃

an RNA molecular-weight marker can be directly applied to native gel, denatured gel, or polyacrylamide gel.

RNA l l i ht k b di tl li d

RNA Step Ladder (0.1–1 kb) (5)

2XLoading

Dye

7

Electrophoresis-related ProductsProtein Size Marker

Prestained PrestainedⅡ Western

For low molecularrange

For middle molecularrange

For high molecularrange

Wide range

Biotinylated1 2 3 4

5 6 7 8

（kDa）（kDa）（kDa）

（kDa）（kDa）（kDa）（kDa）

（kDa）

30

20

15

140

100

70

50

40

79

42

31

21

14

42

30

20176.53.5

200

116

79

42

30

17

1801169779

42

30

20

146.5

79

42

30

2014

150120100

80

60504235

25

16

240140100

70

50

35

2520

15

7

Contains protein G with the ability to combine with the Fc region of immunoglobulin. Reacts with both primary and secondary antibodies in a western blot.

Prestained Marker (1, 2) :

Contains biotinylated protein. Capable of both chromogenetic and photogenetic detection by reacting with avidin HRP or avidin AP.Biotinylated Marker (4) :

Prote

in S

ize M

arker

Western Marker (3) :

With dye combined in its recombinant protein content.

－20℃ －20℃ －20℃

Marker for CBB Dyeing (5~8)Optimized for CBB dyeing. Obtains sharp, uniform bands because of a reduction alkyl process.

12345678

WIDE-VIEW TM Prestained Protein Size MarkerWIDE-VIEW TM Prestained Protein Size Marker 2WIDE-VIEW TM Western Size MarkerWIDE-VIEW TM Biotinylated Size MarkerMolecular Weight Marker, Low Range Molecular Weight Marker, Middle RangeMolecular Weight Marker, High RangeMolecular Weight Marker, Wide Range

230-02221239-02291233-02211233-02331294-63101131-14511134-14501296-63301

500 μL500 μL250 μL 1.5 mL1 mL1 mL1 mL1 mL

Wako Cat. No.Number Product name Package Size

Store at ｰ20℃Store at 2～10℃

　　　　　　:

NO DISPLAY:

－20℃

8

Easy Set Easy Remove

1 2 3

a

b

c

d

rat muscle glycogen phosphorylase (EC2.4.1.1)

relative abundance

Electrophoresis-related ProductsElectrophoresis-related Products

Easy to operate.

Requires only a small amount (about 250-350ml) of running buffer for electrophoresis.

Allows the gel plate to be removed without removing the running buffer after electrophoresis has been completed.

Features

12

3

Detectable limit of protein: 1 ng

Sensitive and Simple procedure with 2-step staining in 5~10 minutes

Optimized the staining intensity by either de-staining the excess stain or re-staining following de-stain of the gel.

Applicable to proteome analysis by mass spectrometry and sequencing

Featureas

12

3

4

Easy SeparatorTM (Wako Catalog No.058-07681 (1 set ) )

Easy SeparatorTM is an electrophoresis tank specially designed for “SuperSep” products to enable easy setting and removal of the gel plate.

Negative Gel Stain MS KitProtein bands separated by SDS/polyacrylamide gel electrophoresis (SDS-PAGE) can be visualized as stable, clear and transparent bands against the background of milky white gel stained by negative gel stain containing our improved imidazol derivative reagent in as little as 10 minutes. The staining technique is useful to obtain the clear and sensitive resolution pattern of the gel before immunoblotting as well as to excise and purify the band of interest from the gel without significant deterioration of amino acid residues for the subsequent studies of protein such as sequencing and mass analysis of peptide.

①Remove the cover after electrophoresis has been completed.

①Insert a gel. ②Turn the knobs toward you.

③Setting is completed.

②Turn the knobs away from you.

③Remove the gel without removing the running buffer.

Electr

ophoresis

-rela

ted P

roducts

Negative Gel Stain MS KitKit Contents:1) Staining S olution A (1 × 500 mL); 2) Staining Solution B (1 × 500 mL); 3)De-staining Solution (1 × 500 mL)

293-57701 20 tests

Wako Cat. No. Product name Package Size

SDS-PAGE analysis

MALDI-TOF/MS analysis

Figure 1. SDS-PAGE analysis of proteins using Negative Gel Stain MS Kit

a : rat phosphorylase (97k);b : bovine serum albumin (68k);c : hen egg white ovalbumin (45k);d : bovine carbonic anhydrase (31k).Lane 1: 50 ng; Lane 2: 100 ng; Lane 3: 150 ng

SDS-PAGE gel

Mass Spectrometry

Staining Sol. A

Staining Sol. B

Shake 5 ~ 10 min.Wash 5 ~ 10 sec. x 3

Shake 10 ~ 60 sec.Wash 1 min. x 3

Check staining intensityof the gel against a sheet of black paper

Procedure

Figure 2.

MALDI-TOF/MS of rat phosphorylase

The band was excised and treated with trypsin. Following the in-gel digestion and preparation, the sample was analyzed on MALDI-TOF mass spectrometer. (These data were provided by Dr. Y. Wada at Osaka Medical Center, Japan.)

1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000m/z

（Store at room temperature）

9

SuperSepTM SuperSepTM Ace

17well13wellSuperSepTM AceSuperSep™ Ace is a polyacrylamide gel of the highest quality, designed to resolve the problems of existing products (SuperSep™ and SuperSep™ HG). Tris-glycine buffer is used in the gel buffer, similar to existing products, but because its pH is neutralized, the gel’s chemical storage stability is improved. Physical storage stability is also improved by using vacuum packaging. And because it does not contain SDS, it can be used in SDS-PAGE and Native-PAGE by changing the running buffer.

① SuperSepTM: 10%

②SuperSepTM Ace: 10%

Plate size

Gel size

Number of wells

Well volume

Total amount of protein＊ (/well)

13 wells

30 μL

3.3～ 6.5 μg

17 wells

25 μL

1.3～ 3.9 μg

100 × 100 × 3 （mm）90 × 85 × 1 （mm）

Product specifications

＊Indicates total amount of protein that can be clearly separated.

Good separation and uniform bands

Can be used with a multi-channel pipette.

Reasonably priced and capable of long-term storage (for 6 months)

Box sticker allows easy identification of type of gel.

Comes in 13-well and 17-well types.

12345

0 1.5 3 4.5 6 7.5 9 10.5 12 (hours)

200

（kDa）

150

10075

50

37

25

20

Improved

separation

near the center!Improved

separation

near the center!

17well13wellSuperSepTM AceSuperSep™ Ace amide gel of the highest qualityis a polyacryla

Electrophoresis-related ProductsElectrophoresis-related Products

Electr

ophoresis

-rela

ted P

roducts

Features

Uniform

band!

Convenient

sticker for

gel

identification!

Convenient

sticker for

gel

identification!

SuperSepTMAce, 6%, 13well SuperSepTMAce, 6%, 17well SuperSepTMAce, 7.5%, 13well SuperSepTMAce, 7.5%, 17well SuperSepTMAce, 10%, 13well SuperSepTMAce, 10%, 17well SuperSepTMAce, 12.5%, 13well SuperSepTMAce, 12.5%, 17well SuperSepTMAce, 15%, 13well SuperSepTMAce, 15%, 17well SuperSepTMAce, 5-12%, 13well SuperSepTMAce, 5-12%, 17well SuperSepTMAce, 5-20%, 13well SuperSepTMAce, 5-20%, 17well SuperSepTMAce, 10-20%, 13well SuperSepTMAce, 10-20%, 17well SuperSepTMAce, 15-20%, 13well （Tricine Gel）

195-15171192-15181198-14941191-14931195-14951192-14961199-14971196-14981193-14991190-15001199-15191192-15201197-15011194-15021191-15031198-15041198-15301

10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.10 ea.

Wako Cat. No. Product name Package Size

（Store at 2～10℃）

10

20-200 bp DNA was clearly separated by using SuperSepTM DNA and Tris-glycine buffer. PCR products under 200 bp can be used for separation from gel, especially when cloning small RNA. 30-200 bp DNA was clearly separated with TBE buffer.

Features

（bp）（bp）（bp）（bp）

1009080706050403020

200150

25

（bp）（bp）

100

50

30

20

EtBr staining EtBr staining

100

200 200150

100

75

50

25

150

100

75

50

25

90

80706050

40

30

20

（bp）

100

50

3020

200150

25

（bp）

1% Agarose SuperSepTM DNA（15%, 17 well）

SuperSepTM DNA（15%, 17 well）

2.5% Agarose

Example of electrophoresis

Improved

separation

near the center!

1

3

2

The acrylamide concentration, degree of cross-linkage, and number of wells are optimized for the electrophoresis of low-molecular-weight DNA.

Cleaner separation image than that obtained with agarose gel.

Ideal for 20-200 bp separation.

Plate size

Gel size

Number of wells

Well volume

100 × 100 × 3 （mm）

90 × 85 × 1 （mm）17 wells

25 μL

Product specifications

①With Tris-Glycine running buffer ②With TBE running buffer

SuperSepTM DNA (15%, 17 wells) (Wako Catalog No.190-15481 (10 ea.) )

Polyacrylamide gel is used in the separation of low-molecular-weight DNA because it has a finer gel mesh structure than agarose. However, the DNA size will vary widely depending on the acrylamide concentration and the degree of cross-linkage of the gel. In SuperSepTM DNA, the acrylamide concentration and the degree of cross-linkage of the gel have been optimized for the separation of low-molecular-weight DNA. This product is therefore effective for the separation of samples with a DNA size from 20-200 bp.

Electrophoresis-related Products

Convenient

sticker for

gel

identification!

EtBr Solution 315-90051 10 mLWako Cat. No.Product name Package Size

（Store at 2～10℃）

Staining Reagent

11

Store at or below ｰ20℃

【How to read the chart】【Display codes】

10 bp 100 bp 1 kbp 10 kbp 100 kbp 200 kbp

DNA Ladders

10000 b 1000 b 100 b 10 b

10000 b 1000 b 100 b 10 b

Protein Size Markers 10 kDa 100 kDa 1000 kDa

10 kDa 100 kDa 1000 kDa

●1 , p2

●1 , p1

●2 , p2●3 , p2

●6 , p2●7 , p2●8 , p2

●1 , p7●2 , p7

●5 , p7

●3 , p7

●1 , p8●2 , p8

●3 , p8●4 , p8

●5 , p8●6 , p8

●7 , p8●8 , p8

10 b

RNA Ladders

●10 , p2●9 , p2

●11 , p2●12 , p2

●17 , p3●18 , p3

●13 , p3●14 , p3

●15 , p3●16 , p3

●19 , p4●20 , p4

●21 , p4●22 , p4

●26 , p4●27 , p4

●23 , p4●24 , p4●25 , p4

●28 , p4●29 , p4●30 , p4

●31 , p5●32 , p5●33 , p5

●40 , p6●41 , p6●42 , p6

●39 , p6

●4 , p7

【How to read the chart】【Display codes】Selection Guide

Number Reference page

Store at 2～10℃NO DISPLAY Store at or below ｰ80℃

Store at or below ｰ70℃

－80℃

－70℃－20℃

●4 , p2●5 , p2

10 bp 100 bp 1 kbp 10 kbp 100 kbp 200 kbp

08X05開01

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09Y3IBF

・This brochure may contain products that cannot be exported to your country due to regulations.・Listed products are intended for laboratory research use only, and not to be used for drug, food or human use.

Documents

Molecular Weight Markers