Embed Size (px)
Citation preview
Curriculum Vitae April 2015
MOHAMMED ARSHAD
EXPERIENCE
Voluntary Trainee – Research Department - King Khaled Eye Specialist Hospital [December 2014 - February 2015]
Responsibilities
Sample Collection (Iris, Cornea, Aqueous Humour, Donor Rims) from Operation Room/Clinical Labs.
Sample processing for RNA Extraction by Snap Freezing DNA Extraction from Blood samples (QIAGEN, Gentra puregene blood kit) RNA Extraction from Corneal tissues (Optimization, RNeasy mini kit) Nanodrop (Thermoscientific 2000/2000c) Primer designing Gel Electrophoresis Polymerase Chain Reaction (Gradient PCR, Hot Star PCR) and its
Optimization (Bio- rad) Purification of PCR product (Column Clean-up) Target/Sanger Gene Sequencing (Applied Bio systems Hitachi 3730 DNA
analyzer) Sequence Analysis (DNASTAR Laser gene Software, Bioinformatics tools) Monitoring cell culture room (maintaining Stocks, monitoring CO2 incubators,
Inverted Microscopes). Dissection of corneal rims by Dissection Microscope (Olympus TH4-200) Monitoring freezers and Refrigerators (4 oC, -20 oC, -80 oC) Periodic Maintenance of Liquid Nitrogen Levels. Weekly cleaning of sequencer Maintaining records of blood, tissue samples from patients Racking of tips, autoclaving liquids, instruments and lab wares. Detecting Antigens (Proteins) in tissue samples using Immunohistochemistry
technique (Autostainer link DACO)
Researcher - Research Centre, College of Science, King Saud University[September 2009 – November 2014]
Samples preparation of biologically active molecules such as indole & omeprazole derivatives, pyrazoles, thiazoles, pyrimidines, etc., for Nuclear Magnetic Resonance
Worked on Autochem analyzer to ascertain Temperature Programmed Reduction and Oxidation values of polymer samples.
Ensuring complete in-process quality control and continuous improvement in process capabilities.
Ordering, monitoring and maintaining records pertaining to chemicals and disposables in the lab.
Technical presentations of samples processed at periodic intervals and deliver training to new graduate students
+966-531085190 [email protected]
Curriculum Vitae April 2015
EDUCATION
Master of Sciences, College of Science, King Saud University, Riyadh, KSAYear of Graduation – 2014; GPA - 4.6/5
Core Module:
Cell Biology Microbial Molecular Genetics Molecular Biology Advanced Bacteriology Mechanism of Bacterial
Infection Advanced Virology
Microbiology Microbial Biotechnology Advanced Mycology
Research Thesis
Thesis Title: Polyhydroxy butyrate (PHB) production by Bacillus sp. isolated from Mangrove environments of eastern coast of Saudi Arabia.
Identification, isolation and screening of PHB producing bacterial strains from Saudi Arabian Mangroves.
Staining using Nile Blue stain and visualizing with fluorescent microscope. Positive samples were cultured with Complex Nitrogen Limiting Medium,
isolation of PHB and characterization using Nuclear Magnetic Resonance Spectroscopy, Fourier Transform Infrared Spectroscopy, and Liquid Chromatography-Mass Spectrometry.
Comparative bioinformatics screening based on Nucleotide Homology & Phylogenetic analysis yielded a bacterial strain (Bacillus thuringiensis) to be synthesizing PHB
Bachelor of Sciences,Bhavans New Science College, Affiliated to Osmania University, Hyderabad, India.Year of Graduation 2008, GPA - 4.0/5
Main Modules:
Genomics & Proteomics Immunology & Molecular Pathogenesis
Molecular Genetics
Biochemistry Microbiology Molecular Biology Industrial Biotechnology Bioinformatics Cell Biology Biopharmaceutical Technology
Food Science & Technology
Molecular Modelling & Drug Design
Project: Worked on Amylase production from Aspergillus niger at Bhavan’s New Science College Laboratory, Hyderabad, India. (2008)
+966-531085190 [email protected]
Curriculum Vitae April 2015
Certified Trainings:
CPR certified (American Heart Association BLS for Healthcare Providers Program, 9/2014- 9/2016).
Graduate Trainee – Molecular Biology & Recombinant DNA TechnologyIndigenese Biotechnology Center, Hyderabad, India. (2007)
Specific targeted sequences were amplified and cloned in a plasmid incorporated in an E coli. These were further cultured and the colonies were screened for expression of the targeted genes in them.
TraineeDeccan Diagnostic Center, Hyderabad, India. (2006)
Basic training for performing and handling human blood and urine samples diagnostic testing. These include tests for - bilirubin, creatin, glucose, total cholesterol, HDL-cholesterol, Triglycerides, LDL-cholesterol, serum creatin kinase.
PUBLICATIONS
A. Al-Arfaj, Arshad M, A. Murugan, Sholkamy E., Ahmed Abdel-Megeed (2015). Extraction and characterization of polyhydroxybutyrates (phb) from Bacillus thuringiensis ksadl127 isolated from mangrove environments of Saudi Arabia. (Submitted to Brazilian Archives of Biology and Technology).
COMPUTING & OTHER SKILLS
Good working knowledge of MS Office – Word, Excel, PowerPoint. Efficient presentation and skills obtained though several presentations during
Graduate study. Organisational skills acquired by execution of mini projects and group lab work.
+966-531085190 [email protected]
Curriculum Vitae April 2015
PERSONAL DETAILS
Address : Flat # 204, Paramount Heights, Tolichowki, HyderabadMobile : +966 531 08 5190Email : [email protected] : IndianDate of Birth : 12th Feb 1988Marital Status : MarriedIqama : 2279221200 (Transferable).
REFERENCES
Dr Alka Mahale Dr Leen Abu SafiehSenior Research Scientist (Stem Cell) Senior Research Scientist (Oncology) Research Department Research DepartmentKing Khaled Eye Specialist Hospital King Khaled Eye Specialist [email protected] [email protected]+966 (11) 4821234 Ext. 3018 +966 (11) 4821234 Ext. 3013
MOHAMMED ARSHAD
+966-531085190 [email protected]
