Thyretain® - Thyroid Stimulating Immunoglobulin Reporter BioAssayThyroid Stimulating Immunoglobulins (TSI) are linked to an autoimmune thyroid

disorder called Graves’ disease1. In this disease, the TSI bind to the thyroid

stimulating hormone (TSH) receptors (TSHR) of the thyroid gland, causing an

over-production of thyroid hormones. This can lead to a number of different

symptoms, making the initial diagnosis of thyroid disorders difficult.2 However,

the specific detection of TSI in conjunction with other clinical determinations

can be used for differential diagnosis of Graves’ disease. Once diagnosed, the

disease has a variety of treatment options.3

Diagnostic Hybrids’ Thyretain specifically detects TSI in a user-friendly format.•Specific,sensitiveandreproducibletestresults

•SimplifiedcellculturetechniquesutilizingDHIfrozencellculturetechnology

•UseofwholeserumeliminatestheneedforIgGpurification,savingtime

and money

•Resultsin3to4hours,aftercellcultureisprepared,assuresaquick

turnaround time in the lab

With a procedure for easy handling, a patented testing technology, specific TSI detection and quick turnaround time, Thyretain offers a superior product coupled with accurate results that improve patient care and laboratory workflow.

A genetically engineered cell line for the specific detection of TSI. - As shown above, Thyretain utilizes a Chinese hamster ovary cell line engineered to express chimeric human TSH receptors and a cAMP-induced luciferase reporter gene (CHO Mc4). The specificity and simplicity of Thyretain allows the user to generate fast, reliable results.

References

1 Takasu N, Yamashiro K, Komiya I, Ochi Y, Sato Y, Nagata A. 2000. Remission of Graves’ hyperthyroidism predicted by smooth decreases of thyroid stimulating antibody and thyrotropin binding inhibitor immunoglobulin during antithyroid drug treatment. Thyroid 10:891-896.

2.Godfrey,J.(2004)TowardOptimalHealth:TheExpertsDiscussThyroidDisfunction.JournalofWomen’sHealth,Vol.13Issue2,p.141-146.

3 Gittoes, NJL, Franklyn, JA. 1998. Hyperthyroidism: Current Treatment Guidelines.Drugs55:543-553.

Function of a CHO Mc4 Cell

A Quidel Company For More Information, Contact Us www.dhiusa.com (866) 344-3477© 2010 Quidel Corporation. All rights reserved.

A Quidel Company For More Information, Contact Us www.dhiusa.com (866) 344-3477© 2010 Quidel Corporation. All rights reserved.

MKT0056v2010AUG11

Catalog Code

40-25000.v2 Thyretain TSI Reporter BioAssay

capable of testing 25 plates

Includes:ThyretainFreshFrozenCells® and Control Set (1),

Thyretain Media Set (1), Luciferase Assay Reagent Set (10)

40-50000.v2 Thyretain TSI Reporter BioAssay

capable of testing 50 plates

Includes:ThyretainFreshFrozenCells® and Control Set (1),

Thyretain Media Set (2), Luciferase Assay Reagent Set (20)

Individual Component

40-E2610.v2 Luciferase Assay Reagent Set

Thyretain Product Codes

Recommended Protocol

Dilute1vialofCHOMc4FreshFrozenCells® in5-mLThyretainGrowthMediumandseedthetreatedwells

Gelatintreatintheinner48wellsofablack,clear-bottom, 96 multi-well plate

Incubatecellsfor15to18hours

Prepare 1/11 dilutions of controls and specimens

Treat cells with prepared controls and specimens

Incubate cells for 3 hours

Lyse cells for 10 minutes and measure luminescence

Specimen≥140%ofthereferencecontrol Specimen<140%ofthereferencecontrol

Specimen is “Positive” Specimen is “Negative”