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Product No. 99 819 Version C 01/2007 mini API ® Service Manual bioMérieux sa 69280 Marcy l'Etoile / France Tel. 33 (0)4 78 87 20 00 - Fax 33 (0)4 78 87 20 90 http://www.biomerieux.com Printed in France / 673 620 399 RCS Lyon © 2006 – 2007 bioMérieux SA

mini API - Frank's Hospital Workshopfrankshospitalworkshop.com/equipment/documents/automated...tel. (64) 9 415 0601 fax (64) 9 415 0603 Norway bioMérieux Norge AS ∅kernveien 145

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  • Product No. 99 819 Version C 01/2007

    mini API®

    Service Manual

    bioMérieux sa 69280 Marcy l'Etoile / France

    Tel. 33 (0)4 78 87 20 00 - Fax 33 (0)4 78 87 20 90 http://www.biomerieux.com

    Printed in France / 673 620 399 RCS Lyon

    © 2006 – 2007 bioMérieux SA

  • 501-1584 A en

    Argentina bioMérieux Argentina Av. Congreso 1745 (C1428BUE) Capital Federal Buenos Aires tel. (54) 11 5555-6800 fax (54) 11 5555-6888

    Australia bioMérieux Australia P/L Unit 25, Parkview Business Center 1 Maitland Place Baulkham Hills NSW 2153 tel. (61) 2 8852 4700 fax (61) 2 8852 4777

    Austria bioMérieux Austria GmbH Eduard-Kittenberger-Gasse 97 Top 3 A-1230 Wien tel. (43) 186 50 650 fax (43) 186 50 661

    Belgium bioMérieux Benelux s.a./n.v. Media Square 18-19 Place des Carabiniers Bruxelles 1030 tel. (32) 2 743 01 70 fax (32) 2 733 55 97

    Brazil bioMérieux Brasil SA Estrada Do Mapua 491 Taquara - Jacarepaguá CEP 22710 261 Rio de Janeiro R.J Rio de Janeiro R.J tel. (55) 21 2444 1400 fax (55) 21 2445 6025

    Canada bioMérieux Canada, Inc. 7815, Henri-Bourassa West Saint Laurent, QC H4S 1P7 tel. (1) 514 336 7321 fax (1) 514 807 0015

    Chile bioMérieux Chile S.A. Seminario 131 Providencia Santiago tel. (56) 2634 20 92 fax (56) 2634 20 93

    China bioMérieux China Limited Room 1601-02B & 10 Est Ocean Centre n° 24A Jiang Guo Men Nei Street, 100004 Beijing tel. (86) 10 6515 6963 fax (86) 10 6515 6993 bioMérieux China Limited Room 2605, South Tower, World Trade Center 371-375 Huan Shi Dong East Road 510095 Guangzhou tel. (86) 20 8762 7010 fax (86) 20 8762 7015

    Colombia bioMérieux Colombia Ltda Avenida 15 No. 100-43 Piso 2 Bogotá D.C. tel. (57) 1 520 0080 fax (57) 1 520 0088 / 1 520 0831

    Czech republic bioMérieux CZ s.r.o. Business Park Kosice, Jinonická 80 158 00 Praha 5 tel. (420) 2 57 290 623 / (420) 2 57 290 232 fax (420) 2 57 290 964

    Denmark bioMérieux Danmark Aps Smedeholm 13C, 2730 Herlev tel. (45) 70 10 84 00 fax (45) 70 10 84 01

    Finland bioMérieux Suomi Oy Konalantie 47 C FI-00390 Helsinki tel. (358) 9 8545 6000 fax (358) 9 8545 6045

    France bioMérieux S.A. 69280 Marcy l’Etoile tel. 33 (0)4 78 87 20 00 fax 33 (0)4 78 87 20 90 http://www.biomerieux.com

    Germany bioMérieux Deutschland GmbH Weberstrasse 8 D-72622 Nürtingen tel. (49) 7022 30070 fax (49) 7022 36110

    Greece bioMérieux Hellas S.A. Papanikoli 70 15232 Halandri Athens tel. (30) 210 81 72 400 fax (30) 210 68 00 880

    Hungary bioMérieux Hungária Kft. Fóti út. 56 (5th Floor) H-1047 Budapest tel. (36) 1 231 3050 fax (36) 1 231 3059

    India bioMérieux India Pvt. Ltd A-32, Mohan Co-Operative Ind. Estate New Delhi 110 024 tel. (91) 11 42 09 88 00 fax (91) 11 24 64 88 30

    Indonesia Representation office bioMérieux Indonesia Enseval Building Kawasan Industri Pulo Gadung - JI. Pulo Lentut No. 10 Jakarta Timur 13920 tel. (62) 21 461 51 11 fax (62) 21 460 41 07

    Italy bioMérieux Italia S.p. A Via Fiume Bianco, 56 00144 Roma tel. (39) 0 6 523081 fax (39) 0 6 52308240

    Ivory Coast bioMérieux Afrique Occidentale 08 BP 2634 Abidjan 08 tel. (225) 22 40 93 93 / (225) 22 40 41 40 fax (225) 22 40 93 94

    Japan bioMérieux Japan, Ltd Seizan Bldg., 12-28 Kita-Ayoama 2-chome Minato-ku, Tokyo 107-0061 tel. (81) 3 5411 87 11 fax (81) 3 5411 87 10

    Korea bioMérieux Korea Co., Ltd 1st & 2nd Floor, Yoosung Building # 830-67 Yoksam-dong, Kangnam ku Seoul 135-080 tel. (82) 2 2188 4700 fax (82) 2 547 6263

  • Mexico bioMérieux México SA de CV Chihuahua 88, col. Progreso México 01080, D.F. tel. (52) 55 5481 9550 fax (52) 55 5616 2245

    Netherlands (The) bioMérieux Benelux BV Boseind 15 P.O. Box 23 5280 AA Boxtel tel. (31) 411 65 48 88 fax (31) 411 65 48 73

    New Zealand bioMérieux New Zealand Ltd 22/10 Airbourne Road North Harbour Auckland tel. (64) 9 415 0601 fax (64) 9 415 0603

    Norway bioMérieux Norge AS ∅kernveien 145 N - 0513 Oslo tel. (47) 23 37 55 50 fax (47) 23 37 55 51

    Philippines (The) Representation office bioMérieux Philippines Rep. Office 11th Floor, Pearlbank Centre 146 Valero Street, Salcedo Village 1227 Makati City tel. (632) 817 7741 fax (632) 812 0896

    Poland bioMérieux Polska Sp. Z.o.o. ul. Zeromskiego 17 01-882 Warsaw tel. (48) 22 569 85 00 fax (48) 22 569 85 54

    Portugal bioMérieux Portugal, Lda. Rua Alto do Montijo, Lotes 1 e 2 - Portela de Carnaxide 2794-070 Carnaxide tel. (351) 21 424 59 80 fax (351) 21 418 32 67

    Russia o.o.o. bioMérieux Petrovsko-Razoumovskii proyezd, 29 127287 Moscow tel. (7) 495 777 27 09 fax (7) 495 777 27 08 / (7) 495 214 95 41

    Spain bioMérieux España S.A. Manual Tovar, 45-47 28034 Madrid tel. (34) 91 358 11 42 fax (34) 91 358 06 29

    Sweden bioMérieux Sverige AB Hantverksvägen 15 436 33 Askim tel. (46) 31 68 84 90 fax (46) 31 68 48 48

    Switzerland bioMérieux Suisse s.a. 51, avenue Blanc Case postale 2150 1211 Genève 2 tel. (41) 22 906 57 60 fax (41) 22 906 57 42

    Taiwan Representation office bioMérieux China Limited - Taiwan Branch RM 608, No. 6-3 Ching Cheng Street Taipei 105 tel. (886) 2 2545 2250 fax (886) 2 2545 0959

    Thaïland bioMérieux Thaïland Ltd Regent House Bldg, 16 th Floor 183 Rajdamri Road, Lumpini, Pathumwan Bangkok 10330 tel. (66) 2 651 98 00 fax (66) 2 651 98 01

    Turkey bioMérieux Diagnostik A.S. Değirmen Sok. Nida Plaza Kat:6 34742 Kozyataği / Istanbul tel. (90) 216 444 00 83 fax (90) 216 373 16 63

    United Kingdom bioMérieux UK Ltd Grafton Way, Basingstoke Hampshire RG22 6HY tel. (44) 1256 461881 fax (44) 1256 816863

    USA bioMérieux, Inc. 100 Rodolphe Street Durham NC 27712 tel. (1) 919 620 20 00 fax (1) 919 620 22 11

    Vietnam Representation office bioMérieux Vietnam Rep. Office Room 4A, 4th Floor Green House Building 62A Pham Ngoc Thach Street, Ward 6 District 3 Ho Chi Minh City tel. (84) 88 209 906 fax (84) 88 209 905

    Distribution in over 130 countries

  • V.B 02/2005

    Information supplied in this manual may be subject to modifications before the products described become available. This manual may contain information or references relating to certain bioMérieux® SA products, software or services which are not available in the country of release; this shall not mean that bioMérieux® SA intends to market such products, software or services in such country To request copies of publications or for any technical request, contact bioMérieux® SA or your local distributor.

    L i a b i l i t y d i s c l a i m e r

    This manual is provided “AS IS” without any warranty, whether express or implied, of merchantability, safety, quality, accuracy, performance of products described on this manual, including an implied warranty of merchantability or fitness for a particular purpose, non infringement of third parties intellectual property rights and against an incidental or direct damage. In no event shall bioMérieux® SA be liable for any direct or indirect damage or consequence related to, arising out of or in connection with, any use of this manual and/or its results by the User and/or any third party. In no event shall this manual be construed as an undertaking of bioMérieux® SA, who reserves the right to modify this manual without notice and shall incur no liability as a result of such modification. This manual is provided for information purposes only and except for the present provisions does not constitute a binding document legally.

    I n t e l l e c t u a l P r o p e r t y

    bioMérieux® SA is the sole owner of copyright, patrimonial rights and any other intellectual property rights in and to this manual and its content subject to possible third parties rights. This manual and its content are protected under the provisions of section L.111-1 and following articles of the French Intellectual Property Code and International Copyright and Author Rights Treaties. The rights to use this manual granted herein are non-exclusive and limited to the extent necessary to use the Software and Instrument. In no event shall the Users be granted herein any other right to use this manual including without limitation, the right to reproduce, represent, adapt or translate all or part of this manual by any mean whatsoever and in any country without the prior written consent of bioMérieux® SA. Any use of this manual other than expressly permitted hereunder shall be prosecuted.

    IMPORTANT! USE OF THIS MANUAL CONSTITUTES ACCEPTANCE OF THE CLAUSES ABOVE MENTIONED.

  • bioMérieux and the blue logo are used, pending and/or registered trademarks belonging to bioMérieux® SA or one of its subsidiaries. This manual refers to the following third parties’ trademarks: IBM is an International Business Machines Corporation trademark in some countries.

  • TABLE OF CONTENTS

    LIST OF FIGURES.........................................................................................................V-1

    1 HOW TO USE THIS MANUAL................................................................................. 1-1 1.1 Introduction..................................................................................................................................... 1-1 1.2 Scope of the manual ...................................................................................................................... 1-2 1.3 Finding topics and procedures ....................................................................................................... 1-3 1.4 How the manual is organised......................................................................................................... 1-4

    2 FUNCTIONAL DESCRIPTION................................................................................. 2-1 2.1 Introduction..................................................................................................................................... 2-1 2.2 Presentation ................................................................................................................................... 2-2 2.3 Introduction to identification and susceptibility testing ................................................................... 2-3 2.4 Description/Aim .............................................................................................................................. 2-4 2.5 General description ........................................................................................................................ 2-5

    2.5.1 Configuration components ...................................................................................................... 2-5 2.6 Principle of operation ..................................................................................................................... 2-7

    2.6.1 Functions ................................................................................................................................ 2-7 2.6.2 Presentation............................................................................................................................ 2-7 2.6.3 Characteristics ........................................................................................................................ 2-7

    2.7 General characteristics .................................................................................................................. 2-9 2.7.1 Environmental conditions........................................................................................................ 2-9 2.7.2 Physical features..................................................................................................................... 2-9 2.7.3 Electrical characteristics ....................................................................................................... 2-10 2.7.4 Characteristics of optical components .................................................................................. 2-10

    2.8 Computer...................................................................................................................................... 2-11 2.9 Central processing unit ................................................................................................................ 2-11 2.10 Software ....................................................................................................................................... 2-11 2.11 Consumables ............................................................................................................................... 2-12

    3 PRELIMINARY INSTRUCTIONS ............................................................................. 3-1 3.1 Recommendations for installation and use .................................................................................... 3-2 3.2 Unpacking the mini API ................................................................................................................. 3-2 3.3 Assembly and installation............................................................................................................... 3-3

    3.3.1 Choosing a location ................................................................................................................ 3-3 3.3.2 Setting up the strip tray ........................................................................................................... 3-5 3.3.3 Connections ............................................................................................................................ 3-5 3.3.4 Preparing for operation ........................................................................................................... 3-5 3.3.5 Adjusting the height of the mini API ......................................................................................... 3-5

    mini API Service Manual IV-1

  • 4 DESCRIPTION OF MODULES.................................................................................4-1 4.1 Introduction..................................................................................................................................... 4-1

    OVERVIEW.....................................................................................................................4-3 4.2 Precautions..................................................................................................................................... 4-5 4.3 Tools 4-5 4.4 Disassembly and assembly ............................................................................................................ 4-6

    mini API..........................................................................................................................4-7 4.5 Cable connections .......................................................................................................................... 4-8 4.6 Principle of operation.................................................................................................................... 4-10

    4.6.1 Colorimetric reading .............................................................................................................. 4-10 4.6.2 Nephelometric reading .......................................................................................................... 4-11

    4.7 Block diagram of analogue digital converter................................................................................. 4-12 4.7.1 Removing the casing (Casing ASSY) = 452 1040A.............................................................. 4-14

    POWER SUPPLY BLOCK ...........................................................................................4-15 4.8 Cable connections ........................................................................................................................ 4-16 4.9 Equipment .................................................................................................................................... 4-17 4.10 Removing the power supply block (451 1324A) .......................................................................... 4-17 4.11 Board power (450 0628A) ............................................................................................................ 4-18

    4.11.1 Location................................................................................................................................. 4-18 4.11.2 Removing the board power ................................................................................................... 4-18

    4.12 Principle of operation (power supply block).................................................................................. 4-20 4.12.1 Power supply circuit operation .............................................................................................. 4-20 4.12.2 Reader power supply ............................................................................................................ 4-20 4.12.3 Computer power supply ........................................................................................................ 4-20

    4.13 Principle of operation (board power) ............................................................................................ 4-21 4.13.1 Filter motor drive circuit ......................................................................................................... 4-21 4.13.2 Tray motor drive circuit.......................................................................................................... 4-22

    4.14 Testing and adjustment ................................................................................................................ 4-23 4.14.1 Reader power supply ............................................................................................................ 4-23 4.14.2 Board power .......................................................................................................................... 4-24 4.14.3 Procedure.............................................................................................................................. 4-25

    OPTICAL BLOCK.........................................................................................................4-27 4.15 Optical block cable connections ................................................................................................... 4-28 4.16 Equipment .................................................................................................................................... 4-29 4.17 Removing the optical block........................................................................................................... 4-29 4.18 Condenser (P/N 452 0409A) ........................................................................................................ 4-30

    4.18.1 Removing the condenser ...................................................................................................... 4-30 4.19 Lamp (P/N 451 0293A)................................................................................................................. 4-31

    4.19.1 Removing the Lamp .............................................................................................................. 4-31 4.19.2 Removing the lamp (cont'd) .................................................................................................. 4-32

    4.20 Board optic acquisition (P/N 450 0631A)...................................................................................... 4-33 4.20.1 Location................................................................................................................................. 4-33 4.20.2 Removing the board optic acquisition ................................................................................... 4-33

    4.21 Board detection tray (Sync Assy) (P/N 450 0630A) ..................................................................... 4-34 4.21.1 Location................................................................................................................................. 4-34 4.21.2 Removing the board detection tray ....................................................................................... 4-34

    IV-2 mini API Service Manual

  • 4.22 Board detection filter holder (P/N 450 0629A) ............................................................................. 4-35 4.22.1 Location ................................................................................................................................ 4-35 4.22.2 Removing the board.............................................................................................................. 4-35

    4.23 Principle of operation ................................................................................................................... 4-36 4.23.1 Lamp states .......................................................................................................................... 4-36 4.23.2 Board optic acquisition.......................................................................................................... 4-37 4.23.3 Board detection tray.............................................................................................................. 4-38 4.23.4 Board detection filter holder .................................................................................................. 4-38

    4.24 Testing and alignment .................................................................................................................. 4-39 4.24.1 Lamp and condenser ............................................................................................................ 4-39 4.24.2 Board optic acquisition.......................................................................................................... 4-43 4.24.3 Board detection tray.............................................................................................................. 4-44

    MANAGEMENT BLOCK.............................................................................................. 4-47 4.25 Cable connections........................................................................................................................ 4-48 4.26 Equipment .................................................................................................................................... 4-49 4.27 Removing the board COM GEST2............................................................................................... 4-49 4.28 Principle of operation ................................................................................................................... 4-50

    4.28.1 RS 232 serial communication ............................................................................................... 4-52 4.28.2 EPROM version communication ........................................................................................... 4-52 4.28.3 Checking the position of the protection rail ........................................................................... 4-52 4.28.4 Initialisation of the reader module ......................................................................................... 4-52 4.28.5 Reading mode....................................................................................................................... 4-52 4.28.6 Reading................................................................................................................................. 4-53 4.28.7 Alarm messages ................................................................................................................... 4-55 4.28.8 Management card ................................................................................................................. 4-57

    BOARD INTERFACE SERIAL LCD CPU .................................................................... 4-59 4.29 Removing the board interface (450 0625A) ................................................................................. 4-61 4.30 Cable connections........................................................................................................................ 4-62 4.31 Principle of operation ................................................................................................................... 4-63

    COMPUTER BLOCK WITH BOARD PCM4862 .......................................................... 4-65 4.32 Cable connections........................................................................................................................ 4-67 4.33 Central processing unit ................................................................................................................ 4-68 4.34 Board CPU PCM 4862 (450 0572A) ............................................................................................ 4-69 4.35 Introduction................................................................................................................................... 4-70

    4.35.1 Safety precautions ................................................................................................................ 4-70 4.35.2 Removing the board CPU PCM 4862 (450 0572A) .............................................................. 4-70

    4.36 Specification................................................................................................................................. 4-71 4.36.1 Standard SBC functions........................................................................................................ 4-71

    4.37 Jumpers and connectors.............................................................................................................. 4-74 4.38 SIMM memory modules ............................................................................................................... 4-78 4.39 Detailed information on serial ports.............................................................................................. 4-79

    4.39.1 RS-232 port connector (CN13) ............................................................................................. 4-79 4.39.2 RS-232/422/485 Serial Port (CN8) ....................................................................................... 4-80

    4.40 Jumper settings............................................................................................................................ 4-81

    mini API Service Manual IV-3

  • COMPUTER BLOCK WITH BOARD PCM4860...........................................................4-85 4.41 Cable connections ........................................................................................................................ 4-87 4.42 Central processing unit................................................................................................................. 4-88 4.43 Board CPU PCM 4860 ................................................................................................................. 4-89 4.44 Introduction................................................................................................................................... 4-90

    4.44.1 Safety precautions................................................................................................................. 4-90 4.44.2 Removing the board CPU PCM 4860 ................................................................................... 4-90

    4.45 Specifications ............................................................................................................................... 4-91 4.45.1 Standard SBC functions........................................................................................................ 4-91

    4.46 Jumpers and connectors .............................................................................................................. 4-94 4.47 SIMM memory modules................................................................................................................ 4-97 4.48 Detailed information on serial ports .............................................................................................. 4-98

    4.48.1 RS-232 port connector (JP14)............................................................................................... 4-98 4.48.2 RS-232/422/485 Serial Port (JP9)......................................................................................... 4-99

    4.49 Jumper settings .......................................................................................................................... 4-100 4.50 Floppy drive ................................................................................................................................ 4-101

    4.50.1 Features .............................................................................................................................. 4-101 4.50.2 Removing the floppy drive and hard disk ............................................................................ 4-101

    4.51 Hard disk .................................................................................................................................... 4-102 4.51.1 Features .............................................................................................................................. 4-102

    4.52 Display........................................................................................................................................ 4-104 4.52.1 Mechanical data .................................................................................................................. 4-104 4.52.2 Removing the display (451 1023A) ..................................................................................... 4-104

    4.53 Cable connections ...................................................................................................................... 4-105 4.53.1 Electrical absolute maximum ratings................................................................................... 4-106 4.53.2 Environmental absolute maximum ratings .......................................................................... 4-106 4.53.3 Electrical characteristics of backlight .................................................................................. 4-107 4.53.4 Precautions for use ............................................................................................................. 4-107 4.53.5 Storage................................................................................................................................ 4-107 4.53.6 Safety .................................................................................................................................. 4-107

    PRINTER BLOCK.......................................................................................................4-109 4.54 Outline ........................................................................................................................................ 4-111

    4.54.1 Removing the printer ........................................................................................................... 4-111 4.55 Part functional description .......................................................................................................... 4-112 4.56 General specifications ................................................................................................................ 4-113 4.57 Connectors and signals .............................................................................................................. 4-115 4.58 Setting of DIP switches............................................................................................................... 4-116 4.59 mini API printer setting .............................................................................................................. 4-117 4.60 Printer interface box (451 1024a) ............................................................................................... 4-117 4.61 Printer cable connections ........................................................................................................... 4-118

    IV-4 mini API Service Manual

  • 5 PREVENTIVE MAINTENANCE ............................................................................... 5-1 5.1 Introduction..................................................................................................................................... 5-1 5.2 Testing the mini API ...................................................................................................................... 5-2 5.3 Preventive maintenance................................................................................................................. 5-3

    5.3.1 Cleaning the mini API®........................................................................................................... 5-3 5.3.2 Changing the ribbon................................................................................................................ 5-9 5.3.3 Changing the roll of paper..................................................................................................... 5-10

    5.4 Changing the fuse ........................................................................................................................ 5-12 5.5 Field engineer preventive maintenance ....................................................................................... 5-13

    6 TROUBLESHOOTING GUIDE................................................................................. 6-1 6.1 Introduction..................................................................................................................................... 6-1 6.2 Overview ........................................................................................................................................ 6-2

    6.2.1 Determining the problem......................................................................................................... 6-2 6.2.2 Solving the problem ................................................................................................................ 6-2

    6.3 Error message troubleshooting ...................................................................................................... 6-3 6.3.1 Error messages appearing in the mini API software.............................................................. 6-3 6.3.2 Reference alarm ..................................................................................................................... 6-4

    6.4 Repair by unit replacement ............................................................................................................ 6-6 6.4.1 Flow chart (1) abnormal operation at power on ...................................................................... 6-7 6.4.2 Flow chart (2) abnormal strip tray operation ........................................................................... 6-8 6.4.3 Flow chart (3) incorrect data sent to the computer ................................................................. 6-9

    7 MODULE SPARE PARTS........................................................................................ 7-1 7.1 Introduction..................................................................................................................................... 7-1 7.2 List of tools ..................................................................................................................................... 7-2 7.3 Electronic board ............................................................................................................................. 7-2 7.4 Electrical parts................................................................................................................................ 7-3 7.5 Mechanical parts ............................................................................................................................ 7-4 7.6 Cables ............................................................................................................................................ 7-4

    8 DIAGRAMS.............................................................................................................. 8-1 8.1 Introduction..................................................................................................................................... 8-1

    9 APPENDIX - MAINTENANCE SOFTWARE .......................................................... 9-13 9.1 Introduction................................................................................................................................... 9-13 9.2 Getting started.............................................................................................................................. 9-14 9.3 Overview ...................................................................................................................................... 9-15

    9.3.1 Function "[F4] Filter Values" ................................................................................................. 9-15 9.3.2 Function "[F1] Config" ........................................................................................................... 9-15 9.3.3 Function "[F2] Adjustment" ................................................................................................... 9-16 9.3.4 Function "[F3] Reading" ........................................................................................................ 9-27

    10 GLOSSARY ........................................................................................................ 10-1

    11 INDEX.................................................................................................................. 11-1

    mini API Service Manual IV-5

  • LIST OF F IGURES

    Page 2. FUNCTIONAL DESCRIPTION Figure 2–1: Using mini API® ............................................................................................2-3 Figure 2–2: Description of the spectral zone ....................................................................2-6 Figure 2–3: Optics layout..................................................................................................2-6 Figure 2–4: Filter wheel ....................................................................................................2-6 Figure 2–5: Configuration components.............................................................................2-8 3. PRELIMINARY INSTRUCTIONS Figure 3–1: Setting up the strip tray..................................................................................3-4 Figure 3–2: Connections...................................................................................................3-4 4. DESCRIPTION OF MODULES Figure 4–1: Cable connections .........................................................................................4-8 Figure 4–2: Calculation of numerical values .....................................................................4-9 Figure 4–3: Transmission graph .....................................................................................4-10 Figure 4–4: Filter wheel ..................................................................................................4-10 Figure 4–5: Analogue-digital converter ...........................................................................4-12 Figure 4–6: Position of casing screws ............................................................................4-13 Figure 4–7: Power supply cable connections .................................................................4-16 Figure 4–8: Removing the power supply block ...............................................................4-17 Figure 4–9: Removing the board power .........................................................................4-18 Figure 4–10: Power supply circuit block diagram ...........................................................4-19 Figure 4–11: Filter motor drive circuit .............................................................................4-21 Figure 4–12: Tray motor drive circuit ..............................................................................4-22 Figure 4–13: Switching power supply card testing and adjustment ................................4-23 Figure 4–14: Board power ..............................................................................................4-24 Figure 4–15: Detection of the tray dt = 200ms (tray speed)............................................4-25 Figure 4–16: Optical block cable connections ................................................................4-28 Figure 4–17: Removing the optical block........................................................................4-29 Figure 4–18: Removing the condenser...........................................................................4-30 Figure 4–19: Removing the lamp....................................................................................4-31 Figure 4–20: Handling the lamp......................................................................................4-32

    mini API Service Manual V-1

  • Figure 4–21: Removing the board optic acquisition ....................................................... 4-33 Figure 4–22: Removing the board detection tray ........................................................... 4-34 Figure 4–23: Removing the board detection filter holder................................................ 4-35 Figure 4–24: Lamp control drive circuit .......................................................................... 4-36 Figure 4–25: Analogical switching.................................................................................. 4-37 Figure 4–26: Board detection tray circuit........................................................................ 4-38 Figure 4–27: Board detection filter holder circuit ............................................................ 4-38 Figure 4–28: Beam alignment ........................................................................................ 4-39 Figure 4–29: Strip code reading..................................................................................... 4-41 Figure 4–30: Oscillogram and holes on the tray............................................................. 4-41 Figure 4–31: Position of the beams ............................................................................... 4-43 Figure 4–32: Alignment of the board detection tray ....................................................... 4-45 Figure 4–33: Management block cable connections ...................................................... 4-48 Figure 4–34: Removing the board COM GEST2............................................................ 4-49 Figure 4–35: Management block diagram...................................................................... 4-51 Figure 4–38: Removing the board interface ................................................................... 4-60 Figure 4–39: Board interface serial LCD CUP cable connections.................................. 4-62 Figure 4–40: Computer cable connections..................................................................... 4-67 Figure 4–41: Removing the board CPU ......................................................................... 4-69 Figure 4–42: PCM-4862 jumpers ................................................................................... 4-73 Figure 4–43: PCM-4862 connectors .............................................................................. 4-76 Figure 4–44: PCM-4862 BIOS Settings ......................................................................... 4-83 Figure 4–45: Computer cable connections..................................................................... 4-87 Figure 4–46: Removing the board CPU ......................................................................... 4-89 Figure 4–47: PCM-4860 jumpers ................................................................................... 4-93 Figure 4–48: PCM-4860 connectors .............................................................................. 4-95 Figure 4–49: Display .................................................................................................... 4-103 Figure 4–50: Display cable connections....................................................................... 4-105 Figure 4–51: Printer ..................................................................................................... 4-110 Figure 4–52: Power supply connector.......................................................................... 4-115 Figure 4–53: DIP switch setting ................................................................................... 4-117 Figure 4–54: Printer cable connections........................................................................ 4-118 Figure 4–55: Printer interface circuit ............................................................................ 4-119

    V-2 mini API Service Manual

  • 5. PEVENTIVE MAINTENANCE Figure 5–1: Strip tray ........................................................................................................5-4 Figure 5–2: Preparing the photodiode cleaning tool .........................................................5-6 Figure 5–3: Cleaning the photodiodes..............................................................................5-6 Figure 5–4: Changing the ribbon and the roll of paper .....................................................5-8 Figure 5–5: Printer cover ..................................................................................................5-8 Figure 5–6: Paper cutter ...................................................................................................5-9 Figure 5–7: Loading the paper........................................................................................5-11

    Figure 5–8: Back panel of the mini API .........................................................................5-12 6. TROUBLESHOOTING Figure 6–1: Optical interrupt of the tray ............................................................................6-5

    TABLES Table A: Recommended tools ..........................................................................................4-5 Table B: Equipment required for maintenance .................................................................4-6 Table C: Limits................................................................................................................4-42 Table D: Symptom and flow chart references...................................................................6-6

    mini API Service Manual V-3

  • 1 HOW TO USE THIS MANUAL

    1 . 1 I N T R O D U C T I O N

    This chapter explains how to use the mini API® Service Manual. It is structured as follows:

    SCOPE OF THE MANUAL..........................................................................1-2 ♦

    FINDING TOPICS AND PROCEDURES.....................................................1-3

    HOW THE MANUAL IS ORGANISED.........................................................1-4

    mini API Service Manual 1-1

  • 1 . 2 S C O P E O F T H E M A N U A L

    The mini API® Service Manual is designed to enable the Field Service Engineers involved in maintenance tasks to accurately diagnose problems in order to repair the mini API. This chapter explains how the manual is organised and how it should be used. The Table of Contents lists all the chapters the manual deals with. Each chapter is subdivided into sections corresponding to a procedure or a precise description. The reply to a question can readily be found by referring to the Table of Contents or the Index at the back of the manual.

    1-2 mini API Service Manual

  • 1 . 3 F I N D I N G T O P I C S A N D P R O C E D U R E S

    The mini API® Service Manual is divided into 12 chapters to help you find what you are looking for.

    Table of contents

    The main table of contents of the manual is located on pages IV-1 to IV-5. It lists each chapter and the procedures or topics they contain.

    List of figures

    Pages V-1 to V-3 contain a list of the figures of the manual.

    Chapter contents

    Each chapter starts with a specific table of contents. Each table of contents shows the page from which the different sub-chapters start, on the right of the page.

    Page footers

    Each page of the manual has a page footer.

    The footers contain the title of the manual and the page number.

    Index

    A standard topical index is located at the back of the manual in chapter 11. It is used to locate a particular description or procedure.

    mini API Service Manual 1-3

  • 1 . 4 H O W T H E M A N U A L I S O R G A N I S E D

    This manual is designed to provide you with step-by-step procedures for proper maintenance of your mini API®. Use this Service Manual when you want background or more detailed information on the mini API components. This chapter explains how the Service Manual is organised and how it should be used.

    FUNCTIONAL DESCRIPTION

    Chapter 2 gives an overview of the mini API. − Instrument functions − Instrument operating principle − Characteristics and specifications

    PRELIMINARY INSTRUCTIONS

    Chapter 3 describes the installation procedure and the procedure for preliminary controls.

    DESCRIPTION OF THE MODULES

    Chapter 4 gives for each module:

    − Its function. − The position on the instrument. − A block diagram. − Diagrams and technical drawings. − Procedures allowing you to assemble/disassemble the module. − Procedures to maintain and repair the instruments.

    PREVENTIVE MAINTENANCE

    Chapter 5 describes the customer's schedule for maintenance as well as the ATI maintenance.

    TROUBLESHOOTING GUIDE

    Chapter 6 describes all possible failures and gives the decision tree.

    1-4 mini API Service Manual

  • MODULE SPARE PARTS

    Chapter 7 gives the list of specific tools required to perform the adjustments and carry out repairs on the instruments.

    It also gives you the list of spare parts.

    DIAGRAMS

    Chapter 8 gives you the block diagrams for the mini API® boards.

    MAINTENANCE SOFTWARE

    Chapter 9 contains the description of the maintenance software used for the reader.

    GLOSSARY

    Chapter 10 contains an alphabetical list of terms used in this manual and their definitions.

    INDEX

    A standard topical index is located at the back of the manual in chapter 11.

    mini API Service Manual 1-5

  • 2 FUNCTIONAL DESCRIPTION

    2 . 1 I N T R O D U C T I O N

    The following topics are presented in this chapter:

    PRESENTATION.........................................................................................2-2 ♦

    INTRODUCTION TO IDENTIFICATION AND SUSCEPTIBILITY TESTING .......................................................................2-3

    DESCRIPTION............................................................................................2-4

    GENERAL DESCRIPTION..........................................................................2-5 - Configuration components - Hardware - Software - Consumables

    PRINCIPLE OF OPERATION .....................................................................2-7 - Functions - Presentation - Characteristics

    GENERAL CHARACTERISTICS.................................................................2-9 - Environmental conditions - Physical features - Electrical characteristics - Characteristics of optical components

    COMPUTER..............................................................................................2-11

    CENTRAL PROCESSING UNIT................................................................2-11

    SOFTWARE ..............................................................................................2-11

    CONSUMABLES.......................................................................................2-12

    mini API Service Manual 2-1

  • 2 . 2 P R E S E N T A T I O N

    The mini API® is an automatic system for bacterial identification and susceptibility testing. It can be divided into four categories of elements:

    hardware

    software

    consumables

    user's manual

    2-2 mini API Service Manual

  • 2 . 3 I N T R O D U C T I O N T O I D E N T I F I C A T I O N A N D S U S C E P T I B I L I T Y T E S T I N G

    Standardisation of the inoculum

    Automate

    ID 32 or rapid ID 32 strips A

    Automatic reading and interpretation.

    mini API Service Manual

    Isolation medium orientation tests

    using the DENSIMAT

    Choice of strips

    Manual technique d technique

    Standardised inoculation using the electronic pipette

    TBTM or rapid ATB strips Visual reading. Entry of biochemical

    profiles via the keyboard. Automatic interpretation.

    mini API

    Figure 2–1: Using mini API®

    2-3

  • 2 . 4 D E S C R I P T I O N / A I M

    The mini API® is designed for the automatic identification and susceptibility testing of ID 32, rapid ID 32, ATBTM and rapid ATB strips. It also enables computer assisted interpretation of API® strips read visually.

    2-4 mini API Service Manual

  • 2 . 5 G E N E R A L D E S C R I P T I O N

    2.5.1 Configuration components

    The mini API® is an automated instrument for identification and susceptibility testing. It consists of: − hardware, − software, − consumables.

    2.5.1.1 Hardware

    The package includes:

    The mini API, a self-contained analytical module enabling: − reading of test strips, − management of results and data, − printing of results obtained.

    a DENSIMAT densitometer to standardise the turbidimetry of the bacterial suspension (please refer to the manual supplied with the densitometer).

    an electronic pipette to dispense the required amount of bacterial suspension into each cupule of the identification and susceptibility test strips (55 µl or 135 µl) (please refer to the manual supplied with the pipette).

    2.5.1.2 Software

    The mini API software enables: − interpretation of data, − interpretation of strip results (identification or susceptibility tests), − expert analysis of susceptibility tests, − storage of results on the hard disk, − printing of results, − extraction of data, − connection with a central computer.

    2.5.1.3 Consumables

    The mini API consumables include: − strips with associated media and reagents (see package inserts), − ribbons and paper for the printer, − pipette tips.

    mini API Service Manual 2-5

  • transmission %

    Wavelength in mm

    Figure 2–2: Description of the spectral zone

    Figure 2–3: Optics layout

    Figure 2–4: Filter wheel

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  • 2 . 6 P R I N C I P L E O F O P E R A T I O N

    2.6.1 Functions − Strip identification by strip decoding − Two types of readings:

    − turbinephelometric − colorimetric

    − Communication with the computer.

    2.6.2 Presentation − Black strip carriage. − Blue protection rail.

    2.6.3 Characteristics

    − Description of the spectral zone. − Reading principle of the test strip reader:

    Turbinephelometric reading Turbinephelometric reading is used for test strips containing assimilation tests:

    Example: ID 32 GN, ID 32 C, ATB UR. A turbinephelometric reading cycle occurs in two stages:

    1- Entry of the strip carriage. Detection of the strip code.

    2- Measurement without a filter. Exit of the strip carriage.

    At the end of the cycle, the result is transmitted to the computer.

    Colorimetric reading Colorimetric reading is used for strips containing chromogenic substrates:

    Example: ID 32 STAPH, rapid ID 32 A, rapid ID 32 E A colorimetric reading cycle occurs in 4 stages:

    1- 1st entry of the strip carriage: Detection of the strip code, Measurement under a K60 filter.

    2- 1st exit of the strip carriage: Measurement under a K40 filter.

    3- 2nd entry of the strip carriage: Measurement under a DTb (blue) filter.

    4- 2nd exit of the strip carriage: Measurement under a DTv (green) filter.

    At the end of the reading cycle, the result is transmitted to the computer.

    mini API Service Manual 2-7

  • 1- mini API® 2- Keyboard 3- Electronic pipette 4- DENSIMAT Densitometer

    Figure 2–5: Configuration components

    2-8 mini API Service Manual

  • 2 . 7 G E N E R A L C H A R A C T E R I S T I C S

    2.7.1 Environmental conditions

    Operating Temperature + 15° C to + 30°C (59° F to 86° F).

    Relative Humidity 10 to 70%, without condensation.

    Maximum altitude for installation 3000 m

    Type of installation Indoor, light industrial.

    Degree of Pollution No. 2

    Safety regulations are respected under environmental conditions defined in paragraph 1.4 of the IEC 61010-1 2nd Edition.

    2.7.2 Physical features

    Dimensions Instrument packed Instrument alone Height: 50 cm (19.7 inches) 34.5 cm (13.6 inches) Width: 60 cm (23.6 inches) 43 cm (17 inches) Depth: 65 cm (25.6 inches) - Protection rail in 46 cm (18.1 inches) - Protection rail out 63 cm (24.8 inches)

    Mass Instrument packed Instrument alone 32 kg (70.41 lb) 25 kg (55.11 lbs)

    mini API Service Manual 2-9

  • 2.7.3 Electrical characteristics

    Class Ι Equipment

    Power Supply 100 - 240 VAC

    The power supply is self-switching.

    Consumption Maximum 2 A from 90 to 120 VAC

    Maximum 1.1 A from 220 to 240 VAC

    Frequency 50 - 60 Hz

    Power Maximum 240 VA

    Safety fuse External (power input block)

    3.15 AT - 250 VAC

    2.7.4 Characteristics of optical components

    Light source: Halogen 12 V 20 W − Central photosensor: BP W21 − Side photosensor: SD 5421 − Strip decoding: BP W34

    2-10 mini API Service Manual

  • 2 . 8 C O M P U T E R

    The purpose of the computer is to control the reader and interpret data through the software. It is connected to the reader to ensure automatic analysis of the strips. It is equipped with peripherals for printing and archiving the files.

    Presentation – a Central Processing Unit, – an external Keyboard, – a Monitor, – a Floppy disk drive, – an integrated Printer, – a parallel Port/serial Port.

    2 . 9 C E N T R A L P R O C E S S I N G U N I T

    Characteristics Serial number greater than INS001882 – CPU board: PCM 4862 (ST486 DX4 100 Mhz) – Floppy disk drive: NEC FD1231H - 1.44 Mb, or other compatible model – Hard disk: MAXTOR 6E040L0 - 40 Gb, or other compatible model

    2 . 1 0 S O F T W A R E

    A specific application developed under Turbopascal. Its main functions are: – communication with the reader, – interpretation of results sent by the reader, – interpretation of strip results – storage of results, – printing of results, – bidirectional interface with a central computer, – management of patient files.

    mini API Service Manual 2-11

  • 2 . 1 1 C O N S U M A B L E S

    Strips Identification strips ∗ conventional identification with 18 to 24-hour incubation for:

    − Gram negative rods − staphyloccci − yeasts

    ∗ rapid identification with 4-hour incubation at 37°C for: − enterobacteria − anaerobes − streptococci

    Susceptibility test strips ∗ conventional susceptibility tests with two breakpoints and 18 to 24 hour

    incubation are possible for most organisms of clinical significance.

    ∗ rapid ATB susceptibility tests with one breakpoint and 4-hour incubation are possible for enterobacteria and staphylococci.

    Ribbon and paper for the printer bioMérieux paper: Product no. 99 720 bioMérieux Ink ribbon: Product no. 99 721

    2-12 mini API Service Manual

  • 3 PRELIMINARY INSTRUCTIONS

    This chapter covers the very first steps you need to take before you can use the mini API®. Procedures found in this chapter are:

    RECOMMENDATIONS FOR INSTALLATION AND USE ...........................3-2 ♦

    UNPACKING THE mini API........................................................................3-2

    ASSEMBLY AND INSTALLATION ..............................................................3-3 - Choosing a location - Setting up the strip tray - Connections - Preparing for operation - Adjusting the height of the mini API

    mini API Service Manual 3-1

  • 3 . 1 R E C O M M E N D A T I O N S F O R I N S T A L L A T I O N A N D U S E

    In order to facilitate the assembly of mini API®, it is recommended to unpack the components and carefully read the instructions below before starting.

    CAUTION! Before unpacking the instruments, it is advisable to have previously planned where they will be placed. Avoid exposing the equipment to direct sunlight, excessive heat, humidity or dust. Only power cords and accessories supplied by bioMérieux SA should be used to connect the instruments. All connections should be performed with the power turned off. Do not use sockets controlled by wall-mounted switches or power programmers. Power cuts can destroy data in the memories of the computer and analyser.

    3 . 2 U N P A C K I N G T H E m i n i A P I

    Before opening the boxes Make sure that no damage has been caused during transportation. •

    If damage has occurred, make a claim to the transport company and notify bioMérieux SA or your local distributor.

    When opening the boxes Make sure that all the items in the packing list have been delivered.

    If possible keep packaging materials in case the mini API instruments have to be moved in the future.

    CAUTION! Any damage directly or indirectly resulting from the transport of the instrument without adequate containers will not be covered by the warranty or maintenance contract.

    3-2 mini API Service Manual

  • 3 . 3 A S S E M B L Y A N D I N S T A L L A T I O N

    This manual contains information and warnings which have to be respected by the purchaser or the leasor to ensure safe operation and to maintain the instruments in good condition.

    DANGER! Any break in the grounding protective conductor inside or outside the equipment or disconnection of the protective ground terminal may render the equipment dangerous. Deliberate interruption of such conductors is forbidden. Opening covers or removing components, except those manoeuverable by hand, may give access to parts that can be dangerous if touched. Prior to any adjustment, replacement, servicing or repair, disconnect the instrument if it has to be opened. After opening, if it is imperative to switch the instrument on, any adjustment, servicing or repair should be performed by a qualified technician, well aware of the possible risks.

    CAUTION! Never load the computer with programs and floppy disks other than those provided by bioMérieux® SA. Should this recommendation not be respected, the computer may become a potential computer virus carrier. Any intervention directly or indirectly resulting from the presence of such a virus cannot be covered by the warranty or the maintenance contract.

    3.3.1 Choosing a location

    CAUTION! Avoid exposing the equipment to direct sunlight, excessive heat, humidity or dust.

    Place the equipment on a flat stable surface allowing : • − access to the ON/OFF switch, − access to the disk drive, − use of the protection rail, − opening of the printer cover, − circulation of air.

    mini API Service Manual 3-3

  • 1- Left-hand roller 2- Optical switch for detection

    of the strip tray 3- Right-hand roller

    Figure 3–1: Setting up the strip tray

    1- Keyboard cable connection 2- Serial port for bidirectional interface 3- Power cord connection 4- Printer connection box 5- Printer "Reset" button 6- Printer

    Figure 3–2

    3-4

    : Connections

    mini API Service Manual

  • 3.3.2 Setting up the strip tray

    • Place the strip tray between rollers 1 and 3 and gently push it inside the reader (see Figure 3–1).

    3.3.3 Connections

    Connect the power cord to the male outlet on the instrument.

    Connect the keyboard cable (see Figure 3–2).

    3.3.4 Preparing for operation

    Prior to switching on the mini API®, make sure that: – The AC plug is connected to a grounded outlet. – Spare fuses with appropriate rating and of specified type are available.

    IMPORTANT! Using repaired fuses and short-circuiting fuses is prohibited.

    CAUTION! In case of intervention by unauthorised personnel, the Supplier rejects any responsibility and reserves the right to cancel the warranty.

    3.3.5 Adjusting the height of the mini API

    The mini API is equipped with feet allowing its height to be adjusted (two positions). At the time of delivery the mini API is set in the highest position. To ensure proper functioning of the instrument the feet should be adjusted at the time of installation.

    To put the mini API in the lowest position, Remove the feet spacers and replace the 4 M5x30 CHC screws with the 4 M5x16 CHC screws provided with the instrument.

    mini API Service Manual 3-5

  • 4 DESCRIPTION OF MODULES

    4 . 1 I N T R O D U C T I O N

    This chapter is structured as follows:

    OVERVIEW ................................................................................................4-3 ♦

    mini API® ....................................................................................................4-7

    POWER SUPPLY BLOCK.........................................................................4-15

    OPTICAL BLOCK......................................................................................4-27

    MANAGEMENT BLOCK............................................................................4-47

    BOARD INTERFACE SERIAL LCD CPU ..................................................4-59

    COMPUTER BLOCK WITH BOARD PCM4862 ........................................4-65

    COMPUTER BLOCK WITH BOARD PCM4860 ........................................4-85

    PRINTER BLOCK....................................................................................4-109

    mini API Service Manual 4-1

  • OVERVIEW

    This section includes:

    PRECAUTIONS...........................................................................................4-5 ♦

    TOOLS ........................................................................................................4-5

    DISASSEMBLY AND ASSEMBLY ..............................................................4-6

    This section describes the functions of each module and gives the positions of the modules on the instrument. It also includes the block diagrams, schematic diagrams, technical drawings and procedures allowing you to disassemble or assemble the modules and make any mechanical, electronic or optical adjustments.

    mini API Service Manual 4-3

  • 4 . 2 P R E C A U T I O N S

    Take the following precautions before you disassemble or assemble the module.

    WARNING! Before disassembling, assembling or adjusting the modules, disconnect the power supply cable from the AC power outlet. Failure to do so may cause physical injury.

    CAUTION! To maintain optimum module operation, use the tools recommended for maintenance work. Adjust each module as described in this manual. Before any of the following procedures can be performed, the mini API® must be partially disassembled.

    4 . 3 T O O L S

    Below is a list of the tools recommended for disassembling, assembling or adjusting the modules. Only use tools that meet these specifications.

    Tools Part No.

    2mm Screwdriver

    1.5mm Hex key

    2.5mm Hex key

    3mm Hex key

    4mm Hex key

    5mm Hex key

    5.5mm Wrench

    7mm Wrench

    60W Soldering iron

    mini API (1525 calibration kit) 70010 Neutral filter 455 0128 B Screwdriver for adjustment

    Tool cleaner 455 0409 A Antistatic wrist strap 455 0272 A

    Table A: Recommended tools

    mini API Service Manual 4-5

  • Description Specifications

    Multimeter (Fluke or equivalent)

    Oscilloscope 20 Mhz mini API maintenance software Product no. 455 0613 A

    Table B: Equipment required for maintenance

    4 . 4 D I S A S S E M B L Y A N D A S S E M B L Y

    This section describes the procedure for disassembling and assembling the main components of each module. In general, you can install a component in each module by simply reversing the procedure for removing it. Therefore this chapter does not give the assembly procedures for all the cases.

    4-6 mini API Service Manual

  • mini API

    This section includes:

    CABLE CONNECTIONS .............................................................................4-8 ♦

    PRINCIPLE OF OPERATION ...................................................................4-10 - Colorimetric reading - Nephelometric reading

    BLOCK DIAGRAM OF ANALOGUE DIGITAL CONVERTER ...................4-12 - Removing the casing (Casing ASSY) = 452 1040A

    mini API Service Manual 4-7

  • 4 . 5 C A B L E C O N N E C T I O N S

    Figure 4–1: Cable connections

    4-8 mini API Service Manual

  • Raw reference measurement Raw value measurement Raw value measurement Sampling Sampling Sampling 112 Acquisitions 56 Acquisitions 5 Acquisitions

    Smoothing curve based on mobile average at level 5:

    '3M=5

    5M+4M+3M+2M+1M'4M=

    56M+5M+4M + '3M+2M

    2Off-ReffRe =′

    fRe209 x Vm'Vr′

    =

    ; etc

    Reference Maximum value Offset Ref Vm Off

    Vm' = Vm - Off

    Restored value:

    Figure 4–2: Calculation of numerical values

    mini API Service Manual 4-9

  • 4 . 6 P R I N C I P L E O F O P E R A T I O N

    4.6.1 Colorimetric reading

    The mini API® measures the transmission of light in four regions of the visible spectrum.

    % of transmission Dtb blue Dtv green

    K60

    K40

    Wavelength (nm)

    Figure 4–3: Transmission graph

    Figure 4–4: Filter wheel

    4-10 mini API Service Manual

  • Each biochemical test is characterised by four numerical values obtained at the end of the reading cycle:

    1st Stage Under a K60 filter Reading of the strip code −

    Measurement of the reference Measurement of the values

    2nd Stage Under a K40 filter Measurement of the reference Measurement of the values

    3rd Stage Under a Dtb blue filter Measurement of the reference Measurement of the values

    4th Stage Under a Dtv green filter Measurement of the reference Measurement of the values

    5th Stage Calculation of the numerical values (see Figure 4–2).

    4.6.2 Nephelometric reading

    For each cupule of the strip the mini API® performs two types of measurements: Turbidimetry: measurement of the intensity of transmitted light (T) which is inversely proportional to the amount of bacterial growth.

    • Nephelometry: measurement of the intensity of scattered light (S) at 30°, directly proportional to the amount of bacterial growth.

    The reader transmits these two values which enable the estimation of the density of bacterial growth in the medium (turbi-nephelometry) according to the following ratio: Turbi-nephelometry = S / T x 256.

    mini API Service Manual 4-11

  • 4 . 7 B L O C K D I A G R A M O F A N A L O G U E D I G I T A L C O N V E R T E R

    Figure 4–5: Analogue-digital converter

    4-12 mini API Service Manual

  • C

    B A

    Figure 4–6: Position of casing screws

    mini API Service Manual 4-13

  • Before any of the following procedures can be performed, the mini API® must be partially disassembled. To reach the proper stage of disassembly, proceed as follows:

    4.7.1 Removing the casing (Casing ASSY) = 452 1040A

    − Pull out the protection rail. − Remove the tray. − Remove the three M5x10 CHC screws from the back panel of the instrument

    (A). − Remove the two M5x10 CHC screws from the front of the instrument (B). − Place your hands flat on either side of the instrument and lift off the casing.

    WARNING! Do not place your fingers underneath the instrument to lift off the casing.

    4-14 mini API Service Manual

  • POWER SUPPLY BLOCK

    This section includes:

    CABLE CONNECTIONS ...........................................................................4-16 ♦

    EQUIPMENT .............................................................................................4-17

    REMOVING THE POWER SUPPLY BLOCK (451 1324A)...............................................................................................4-17

    BOARD POWER (450 0628A) ..................................................................4-18 - Location - Removing the board power

    PRINCIPLE OF OPERATION (POWER SUPPLY BLOCK) .........................4-20 - Power supply circuit operation - Reader power supply - Computer power supply

    PRINCIPLE OF OPERATION (BOARD POWER)..........................................4-21 - Filter motor drive circuit - Tray motor drive circuit

    TESTING AND ADJUSTMENT .................................................................4-23 - Reader power supply - Board power - Procedure

    mini API Service Manual 4-15

  • 4 . 8 C A B L E C O N N E C T I O N S

    Figure 4–7: Power supply cable connections

    4-16 mini API Service Manual

  • 4 . 9 E Q U I P M E N T

    Designation Part Number Board Power 4500628 A Power Supply Block 4511324 A

    4 . 1 0 R E M O V I N G T H E P O W E R S U P P L Y B L O C K ( 4 5 1 1 3 2 4 A )

    − Remove the casing (see page 4-14). − Remove the printer (see page 4-111). − Remove the three M4x8 CHC screws from the back of the instrument and take

    off the back panel. − Disconnect the power supply cable on the hard disk and the floppy disk. − Disconnect the cables from connectors J3 and J9 on the board COM GEST2. − Disconnect the cables from connectors JC4 and JC5 on the board power. − Remove the four M4x8 CHC screws located on the right and left-hand sides of

    the power supply block. − Remove the power supply block.

    Power supply block

    4 M3X8 CHC Screws Bottom

    Figure 4–8: Removing the power supply block

    mini API Service Manual 4-17

  • 4 . 1 1 B O A R D P O W E R ( 4 5 0 0 6 2 8 A )

    4.11.1 Location

    This board is fastened to the front part of the power supply block.

    4.11.2 Removing the board power

    It is not necessary to remove the power supply block in order to remove the board power. − Remove the casing (see page 4-14). − Remove the printer (see page 4-111). − Disconnect the cables from connectors JC1, B1, JC4 and JC5 on the board

    power. − Disconnect the cables from connector J3 on the COM GEST2 board. − Remove the three M3x6 CHC screws on the board power. − Remove the board power.

    Power supply block

    3 CHC (M3X8)

    Board power Bottom

    Figure 4–9: Removing the board power

    4-18 mini API Service Manual

  • Computer power supply

    Smoothing Circuit

    Full Wave Rectification Circuit

    +12V switching regulator

    +5V switching regulator

    +12V integrated regulator

    Printer

    Floppy disk

    Board CPU

    Hard disk

    Filter

    circuit

    AC

    Input

    +5V regulator

    +15V regulator

    -15V regulator

    +5V regulator

    -12V regulator

    +12V regulator

    FAN

    +24V adjustable regulator

    +11,5V Lamp adjustable regulator

    To B1 on Board power Full Wave Rectification Circuit

    Smoothing Circuit

    Switching Circuit Photo coupler

    Current Overload Protection Circuit

    Switching Circuit

    Photo coupler Current Overload Protection Circuit

    To J9 on Com Gest 2

    To JCI on on Board power

    Inhibit and Prog

    From JC1 on Board powerTemp control

    Reader power supply Figure 4–10: Power supply circuit block diagram

    mini API Service Manual 4-19

  • 4 . 1 2 P R I N C I P L E O F O P E R A T I O N ( P O W E R S U P P L Y B L O C K )

    4.12.1 Power supply circuit operation

    Figure 4–10 shows the power supply circuitry in the form of a block diagram.

    When AC power enters mini API® from an external power source, the filter circuit removes the noise.

    4.12.2 Reader power supply

    It provides the board COM GEST2 with stabilised +5VDC and +/- 15VDC from the 220V mains. It provides the board power with stabilised +5VDC and +/- 12VDC. The AC voltage then undergoes full wave rectification and is smoothed to produce direct current. A voltage detector circuit is connected to the switching circuit. This feedback control arrangement ensures a stable voltage supply. An automatic electronic current-limiting circuit with automatic recovery limits short-circuit output current to a safe preset value, protecting load and power supply when direct shorts occur. It provides the board power with +24VDC to supply motors and +11,5VDC to supply the lamp.

    4.12.3 Computer power supply

    It provides the board CPU, hard disk and floppy drive with stabilised +5VDC and +12VDC. It provides the printer with +12VDC. An automatic electronic current-limiting circuit with automatic recovery limits short-circuit output current to a safe preset value, protecting load and power supply when direct shorts occur.

    4-20 mini API Service Manual

  • 4 . 1 3 P R I N C I P L E O F O P E R A T I O N ( B O A R D P O W E R )

    The board power has two main functions:

    4.13.1 Filter motor drive circuit

    The filter motor, which is a bipolar stepper motor, is used to change the filter during a colorimetric reading. It advances 3.6° per step. COM GEST2 U13 circuit ports (PB2, PB4, PB7) are used to control the filter motor. The filter motor drive circuit (GS D200S) is a complete controller and driver that incorporates all the power functions to directly interface a microprocessor and a two-phase permanent magnet motor. Very little information is sent by the microprocessor to the module.

    Step clock •

    Direction (clockwise or counterclockwise CC/CCW).

    Reset and enable. Based on this information, the module generates the proper four-phase sequence to directly drive a two-phase bipolar motor. The GS D200S incorporates a thermal protection that switches off the power stages when the junction temperature of active components reaches 150°C.

    Figure 4–11: Filter motor drive circuit

    mini API Service Manual 4-21

  • 4.13.2 Tray motor drive circuit

    The tray motor (continuous motor) is used to move the tray in two directions. The P2 potentiometer allows you to adjust the output voltage to drive the motor and thus adjust the speed of the tray. COM GEST2 U13 circuit ports (PB3, PB5, PB1) are used to control the tray motor.

    Figure 4–12: Tray motor drive circuit

    4-22 mini API Service Manual

  • 4 . 1 4 T E S T I N G A N D A D J U S T M E N T

    Material required: − Digital multimeter (Fluke 8050A or equivalent). − Screwdriver for adjustment. − mini API maintenance software (product no. 455 0613 A).

    4.14.1 Reader power supply

    Adjustment procedure: +24VDC Adjustment − Connect DMM between PT6 (Gnd) and PT1 (24V) on the board power. − Adjust with the RV1 potentiometer on the power supply block (see Figure 4–13)

    so that the voltage is between 24VDC +/- 0.1V.

    Output voltage Test Point Board Notes Max. intensity+5V ± 50 mV PT25-PT24 COMGEST Non-Adj 1.5A

    +15V ± 500 mV PT17-PT26 COMGEST Non-Adj 0.3A -15V ± 500 mV PT17-PT27 COMGEST Non-Adj 0.3A +24V ± 500 mV PT1-PT6 POWER Adj. with RV1 1.5A +12V ± 500 mV PT6-PT8 POWER Non-Adj 2.2A -12V ± 500 mV PT6-PT11 POWER Non-Adj 2.2A

    +11,5 VDC Lamp Adjustment To adjust the lamp, use the maintenance software (see chapter 10). − Adjust RV2 on the power supply block to obtain 11.5V +/- 0.1V.

    CAUTION! Take measurements directly from the lamp pins on the lamp holder board.

    Power supply block

    RV1

    RV2

    Figure 4–13: Switching power supply card testing and adjustment

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  • 4.14.2 Board power

    Pin 13 and pin 18 of the IC11: L293E allow you to control the supply voltage of the tray motor (continuous motor) which is adjusted using P2 on the board power. This verification is possible on pin 1 and 3 of the B1 connector on the board 450 0283A. PT3 is not used during the voltage adjustment. The FU1 fuse protects the +24V on the GS D200S chip against overloading. − T 1A Slow blow fuse.

    Fuse

    Tray speed adjustment

    Figure 4–14: Board power

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  • 4.14.3 Procedure

    After initialisation of the system and the mini API® maintenance software (see chapter 9), follow the instructions below. On the board COM GEST2:

    Ground of the oscilloscope to PT17. •

    • Channel A of the oscilloscope to PT33 setup of oscilloscope 2V/div 100ms/div.

    − Start a reading using F3 and Color imetr ic in the ATB / mini API maintenance software.

    − Adjust P2 in order to obtain about 200 ms between the gaps of the tray. − Repeat the operation until you obtain the correct value. Note: Adjustment with frequencies digital meter. The target is 4.8 to 5.2 Hz (4.95 Hz average

    between IN and OUT tray movements).

    Figure 4–15: Detection of the tray dt = 200ms (tray speed)

    mini API Service Manual 4-25

  • OPTICAL BLOCK

    This section includes:

    OPTICAL BLOCK CABLE CONNECTIONS..............................................4-28 ♦

    EQUIPMENT .............................................................................................4-29

    REMOVING THE OPTICAL BLOCK .........................................................4-29

    CONDENSER (P/N 452 0409A) ................................................................4-30 - Removing the condenser

    LAMP (P/N 451 0293A) .............................................................................4-31 - Removing the Lamp - Removing the lamp (cont'd)

    BOARD OPTIC ACQUISITION (P/N 450 0631A)...........................................4-33 - Location - Removing the board optic acquisition

    BOARD DETECTION TRAY (SYNC ASSY) (P/N 450 0630A).....................4-34 - Location - Removing the board detection tray

    BOARD DETECTION FILTER HOLDER (P/N 450 0629A) .......................4-35 - Location - Removing the board

    PRINCIPLE OF OPERATION ...................................................................4-36 - Lamp states - Board optic acquisition - Board detection tray - Board detection filter holder

    TESTING AND ALIGNMENT ....................................................................4-39 - Lamp and condenser - Board optic acquisition - Board detection tray

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  • 4 . 1 5 O P T I C A L B L O C K C A B L E C O N N E C T I O N S

    Figure 4–16: Optical block cable connections

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  • 4 . 1 6 E Q U I P M E N T

    Designation Part Number Condenser 452 0409A StripTray 452 0406A Tray Motor ASSY 451 1031A Filter Motor ASSY 451 1030A Board Detection Tray 450 0630A Lamp Holder Board 450 0283A 12V 20W Tungsten Lamp 451 0293A Board Detection Filter Holder 450 0629A Board Optic Acquisition 450 0631A Anticaloric Filter 452 0361A DTb Blue Filter 452 0365A DTv Green Filter 452 0364A K60 Filter 452 0363A K40 Filter 452 0362A

    4 . 1 7 R E M O V I N G T H E O P T I C A L B L O C K − Remove the tray. − Remove the casing (see page 4-14). − Remove the printer (see page 4-111). − Remove the earth wire on the tray motor assembly (A). − Remove the earth wire on the board optic acquisition from the bottom of the

    instrument (B). − Disconnect the cables from connectors J4 and JC5 on the board power. − Disconnect the cable from connector JC1 on the board detection tray. − Disconnect the cable from connector J4 on the board COM GEST2. − Remove the four HM5 nuts (C) from the bottom of the mini API® (see page

    4-14). − Remove the optical block.

    Figure 4–17: Removing the optical block

    B A

    mini API Service Manual 4-29

  • 4 . 1 8 C O N D E N S E R ( P / N 4 5 2 0 4 0 9 A )

    4.18.1 Removing the condenser

    − Remove the casing (see page 4-14). − Remove the printer (see page 4-111). − Remove the optical block (see page 4-29). − Remove the two CHC (M3X8) screws on the protection plate (A). − Remove the protection plate. − Disconnect the cable from connector B1 on the lamp holder board. − Remove the two CHC (M3X8) screws on the condenser (B). − Remove the condenser by pulling it away from the optical block.

    A B

    Figure 4–18: Removing the condenser

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  • 4 . 1 9 L A M P ( P / N 4 5 1 0 2 9 3 A )

    4.19.1 Removing the Lamp

    − Remove the casing (see page 4-14). − Remove the printer (see page 4-111). − Remove the two CHC (M4X10) screws on the lamp holder board. − Remove the lamp holder board. − Untighten the CHC (M3X6) screws on the square lamp support. − Untighten the CHC (M2X10) screws on the block fixing the lamp. − Remove the lamp.

    Lamp CHC (M3X6)

    Lamp Holder Card

    Square Lamp Support CHC (M2X10)

    Condenser

    Board Detection Tray

    Tray

    Figure 4–19: Removing the lamp

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  • 4.19.2 Removing the lamp (cont'd)

    CAUTION! The lamp must be handled with care. Do not touch it directly with your fingers.

    350°C

    Figure 4–20: Handling the lamp

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  • 4 . 2 0 B O A R D O P T I C A C Q U I S I T I O N ( P / N 4 5 0 0 6 3 1 A )

    4.20.1 Location

    The board is fastened underneath the optical block.

    4.20.2 Removing the board optic acquisition

    − Remove the casing (see page 4-14). − Remove the optical block (see page 4-29). − Remove the three CHC (M3X6) screws underneath the optical block (A). − Remove the board.

    A

    Figure 4–21: Removing the board optic acquisition

    mini API Service Manual 4-33

  • 4 . 2 1 B O A R D D E T E C T I O N T R A Y ( S Y N C A S S Y ) ( P / N 4 5 0 0 6 3 0 A )

    4.21.1 Location

    The board is fastened on the left-hand side of the lower plate of the optical block.

    4.21.2 Removing the board