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min0 0.25 0.5 0.75 1 1.25 1.5 1.75 2
mAU
0255075
100125150175
mAU
min0 0.25 0.5 0.75 1 1.25 1.5 1.75 20
25
50
75
100
125
150
175
min0 0.25 0.5 0.75 1 1.25 1.5 1.75 2
mAU
0
25
50
75
100
125
150
175
3.5 um
5 um
12
3
45 6
7
12
3
4
56
7
12
3
45
67
1 4-Acetamidophenol2 Caffeine3 2-Acetamidophenol4 Acetanilide5 Acetylsalicylic Acid6 Phenacetin7 Salicylic Acid
LC ConditionsColumn: SB-C18, 4.6 x 30 mmDetector: 254 nmInjector: 1 ul, Mobile Phase: 1% Formic Acid Acetonitrile (82:18)Flow: 2.0 ml/min
1.8 um
Aumento della Risoluzione in funzione del Particle Size
0,0
0,2
0,4
0,6
0,8
1,0
1,2
1,4
1,6
1,8
2,0
2,2
2,4
2,6
2,8
3,0
3,2
3,4
3,6
3,8
4,0
Particle Size (um)
Meno particellepiù piccole
Piccola quantità di particelle più grandi
min1 2 3 4 5 6 70
1.8µm: Rs(4,5) = 5.45Peak Width 5s(9) = 0.075
5µm: Rs(4,5) = 3.63Peak Width, 5s (9) = 0.103
Rs = +50%
Aumento della Risoluzione in funzione del Particle Size
+ 50% Resolution (Theory: + 67%)+ 125% Efficiency (Theory: + 177%)
Colonna SB-C18 RRHT 4.6 x150mm
Massima lunghezza
Rs(1,2) = 3.1
0.5 1 1.5 2 2.5
12 34
.
4.6 x 250 mm, 5 m1 mL/min
min0 5 10 15 20 25 30
4.6 x 100 mm, 3.5 m1 mL/min
4.6 x 30 mm, 1.8 m 1 mL/min
4.6 x 30 mm, 1.8 m2 mL/min
min0 5 10 15 20 25 30
min0 5 10 15 20 25 30
min0 5 10 15 20 25 30
29.65
12.71
4.15
2.09
1 23
1
1
2
2
3
3
4
4
4
Rs(1,2) = 4.8
Rs(1,2) = 3.5
Rs(1,2) = 3.3
N=22680
N=11691
N=6104
N=6568
N=6460
N=6463
N=21848
1100 HT + 1.8um RRHT : • Reduction of analysis time from 30 min to 2 min
• Speed: 14x faster than 4.6 x 250mm, 5um
• Time saving: 92%• Resolution: -35%
AUMENTO DELLA VELOCITA’ D’ANALISI CON HPLC 1100
Speed
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0Time (m in)
1
23
4
5 67
1
23
4
56
7
1
23
4
5 6
7
Sample: 7 AnalgesicsColumn: Zorbax RRHT SB-C18,
1.8 um, 4.6 x 30 mm
Rs2,1 = 2.1
Rs2,1 = 2.2
Rs7,6 = 2.2
Rs7,6 = 2.2
Higher flow rate:
higher speed – same resolution – higher sensitivity/precision
2 ml/min – 90bar
3 ml/min – 133bar
4 ml/min – 181bar
2x faster than 2ml/min without loss in resolution!
Ultra-fast analysis in 26 sec4 peak width ~ 0.6 sec
< 50 sec cycle time
7 analgesics separated in 21 secfor peaks 1,2,3Total time Injection-to-Injection
HPLC, 40°CSB-C184.6 x 150mm, 5µm1.20ml/min, 40°CAnalysis Time = 11min
min0 2 4 6 8 10
Rapid Resolution HT Columns – Up to 20X FasterRapid Resolution HT Columns – Up to 20X Faster
RRHT SB-C182.1mm x 50mm 1.8µm1.00ml/min, 40°CAnalysis Time= 1.1min
RRLC, 40°C 10x faster
min0.2 0.4 0.6 0.8 10
0.2 0.60 0.4 min
RRHT SB-C182.1mm x 50mm 1.8µm2.40ml/min, 95°CAnalysis Time: 0.4min
RRLC, 95°C
27x faster >20X faster>20X faster
Up to 20x faster than conventional HPLC • With same or better performance (resolution, precision, sensitivity, carry over)• Compliant with strictest regulatory performance requirements
min1 2 3 4 5 6 70
4
5
1.8µm: Rs(4,5) = 5.45Peak Width 5s(9) = 0.075
5µm: Rs(4,5) = 3.63Peak Width, 5s (9) = 0.103
Rs = +50%
NON SOLO VELOCITA’ MA ANCHE EFFICIENZA
+ 50% Resolution (Theory: + 67%)+ 125% Efficiency (Theory: + 177%)
Colonna SB-C18 RRHT 4.6 x150mm
Massima lunghezza