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Michael D. Boyle
1
CURRICULUM VITAE
Name: Michael D. P. Boyle
Education:
1960-1967 Royal Belfast Academical Institution, Belfast, Northern Ireland
1967-1971 B.Sc. (with honors) Biochemistry, University of Glasgow, Glasgow, Scotland
1971-1974 Ph.D., Chester Beatty Research Institute, Belmont, Sutton, Surrey, England
(Thesis: Mechanism(s) of Tumor Cell Destruction by Alloimmune
Macrophages, In Vitro)
Brief Chronology of Employment:
1974-1976 Visiting Fellow, Humoral Immunity Section, Laboratory
of Immunobiology (formerly Immunochemistry Section,
Biology Branch), DCBD, National Cancer Institute, NIH,
Bethesda, Maryland
1976-1980 Expert, Humoral Immunity Section, Laboratory of
Immunobiology, DCBD, National Cancer Institute, NIH,
Bethesda, Maryland
1980 Visiting Scientist, Humoral Immunity Section, Laboratory of
Immunobiology, DCBD, National Cancer Institute, NIH
Bethesda, Maryland
1981-1985 Associate Professor, Department of Immunology and
Medical Microbiology, (Tenure 1983)
Associate Professor, Department of Pediatrics
University of Florida, College of Medicine,
Gainesville, Florida
1985-1988 Professor, Department of Immunology and Medical Microbiology,
Professor, Department of Pediatrics
University of Florida, College of Medicine, Gainesville, Florida
1988-1990 Adjunct Professor, Department of Immunology and
Medical Microbiology, Professor, Department of Pediatrics
University of Florida, College of Medicine
Gainesville, Florida
1988-2002 Professor, Department of Microbiology and Immunology
Medical College of Ohio, Toledo, Ohio
11/96-4/97 Visiting Professor, Department of Medicine, University of Florida,
Gainesville, Florida
1998-2002 Adjunct Professor of Bioengineering, Department of Bioengineering,
The University of Toledo College of Engineering
Michael D. Boyle
2
2002-2012 William J. von Liebig Professor of Biomedical Sciences, Department of
Biology, Juniata College, Huntingdon, Pennsylvania.
Research Interests:
Molecular Pathogenesis
Immunochemistry
Biotechniques
Wound Healing
Tumor Biology
Honors and Other Special Scientific Recognition
1971-1974 Holder of Institute of Cancer Research Studentship
1976-1990 Member, Foundation for the Advancement of Education in the Sciences
1977-present Member, American Association of Immunologists
1977-1992 Member, American Association for Cancer Research
1982-1988 Member, Southeastern Immunology Conference
1984 Stop! Children's Cancer Service Award
1985 Recipient of the University of Florida College of Medicine, Basic Research Prize
1985-1990 Member, American Association of Veterinary Immunology
1986-present Member, American Society of Microbiologists
1987 Senior Faculty Research Award, University of Florida, Chapter of Sigma Xi
1988-present Member Lancefield Society
1989-present Full Member Sigma Xi
1995-1997 American Society for Microbiology Foundation Lecturer
1999 Recipient of the Graduate Dean's Award for mentoring.
1999-2002 Alternate Counselor, American Society for Microbiology, Division E
(Immunology)
2003-2004 Counselor, American Society for Microbiology, Division E
(Immunology)
2003-2004 Ad hoc Nominating Committee to elect Members at Large on the Council
Policy Committee, American Society of Microbiology
2004-2005 President Allegheny Branch, American Society of Microbiology.
2008-2009 Co-director of the newly created Juniata College Center for the Scholarship of
Teaching and Learning
2010. Recipient of the Beachley Award for outstanding teaching.
Appointments to University Committees
1981 Search Committee for faculty member in the School of Veterinary Medicine
Michael D. Boyle
3
1983-1985 Member, Senate University of Florida
1984 Search Committee for faculty member in the school of Pharmacy,
University of Florida, College of Pharmacy
1985 Search Committee for faculty member in the School of Veterinary
Medicine University of Florida
1984-1986 Search Committee for Chairman, Department of Immunology and
Medical Microbiology, University of Florida College of Medicine.
1985 Member, Associate Professor Promotion and Tenure Committee
University of Florida College of Medicine
1986-1987 Member Selection Committee for College of Medicine Basic Research
Prize, University of Florida College of Medicine
1987-1988 Member, Associate Professor Promotion and Tenure
Committee, University of Florida, College of Medicine
1988-1989 Member L.C.M.E. Subcommittee on Basic Science and Graduate Education
1993-2002 Department Representative to the Academic Senate, Medical College of Ohio
1995-1996 Member Academic Senate Steering Committee
1995-1999 Member Conflict of Interest Committee; Chairman 1997-1999
1996 Member L.C.M.E. Subcommittee on Clinical Departments
1997-1998 Member Search Committee for Faculty Member in the Department of
Microbiology and Immunology
1997-1998 Member Tenure Committee, Medical College of Ohio
1998 Member Search Committee for Director, Center for Classroom of the Future
1998-1999 Member Evaluation Subcommittee of Medical School Curriculum Committee
1998-1999 Member Search Committee for Assistant Professor of Microbiology and
Immunology
1998-1999 Member Search Committee for Chair, Department of Pathology
1999 Member Selection Committee M.C.O. Research Award
1999-2002 Member Senate Faculty Affairs Committee
2001-2002 Co-Chair, M.C.O. and University of Findlay disaster-preparedness and
bioterrorism working group.
2001-2002 Member M.C.O. Bioterrorism response group.
2002-2009 Member and Chair of the von Liebig Committee.
2006-2012 Member of the Honorary Degree Committee Juniata College.
2009-2012 Convener of the Juniata College grants group.
Editorial and Peer-review Activities
Editor
Michael D. Boyle
4
1990 Michael D. P. Boyle, Editor, Bacterial immunoglobulin-binding proteins,
Volumes I and II, Academic Press, San Diego, California.
1993 Michael D. P. Boyle, Editor, Bacterial IgG-binding proteins, ImmunoMethods.
1993 Michael D. P. Boyle, Editor, Analysis of host-pathogen interactions. Implications for
pathogenesis, Special Issue Journal of Microbiological Methods.
2000 Michael D. P. Boyle and Richard Lottenberg, Co-Editors, Analysis of the role of the
plasminogen system in microbial pathogenesis, Methods, in press.
Editorial boards
1983-1987 Associate Editor, Molecular and Cellular Biochemistry
1986-2009 Member, Editorial Board, Journal of Microbiological Methods
1986-2012 Member Editorial Board Biotechniques
1989-1990 Member Research Section Editorial Board, Diagnostics and Clinical Testing
1993-2012 Member, Editorial Board, Methods (formerly ImmunoMethods)
Journal Review
Ad Hoc reviewer
Journal of Immunology Molecular Immunology
Journal of Infectious Disease Science
Journal of Immunological Methods Cancer Research
Infection and Immunity Gene
Journal of the National Cancer Institute Protein Engineering
Canadian Journal of Microbiology Journal of Bacteriology
European Journal of Biochemistry Microbiology
Biochemistry Proteomics
Proceedings of the National Academy of Sciences
Grant review:
Member N.C.I. Program Project Site Visit team, University of Philadelphia, 1989
Member N.I.H. Special Experimental Immunology Study Section, August 1988
Member NIDR Study Section for Research Centers in Oral Biology, 1992-1993
Member N.I.H. SBIR Study Section, September 1992
Member NIH SBIR Study Section, November 2000
Member American Heart Association Southern and Ohio Valley Research Consortium Peer
Review Committee 2001
Ad Hoc Reviewer:
N.I.H. - Bacteriology and Mycology Study Section
N.S.F. -Cellular Physiology Section; Engineering Directorate; S.B.I.R. program
United States Department of Agriculture
Michael D. Boyle
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American Heart Association, National; Florida Affiliate; Ohio Affiliate
American Cancer Society, National; Florida Affiliate; Ohio Affiliate
March of Dimes
Veterans Administration Merit Review Program
Department of Energy CRDA-program
Wellcome Foundation
Israeli Medical Research Council
Pennsylvania Greenhouse
Undergraduate medical and allied health education
Organizational activities
1981-1988 Joint Coordinator, Principles of Immunology for Graduate
Students, University of Florida, College of Medicine
1981-1988 Joint Coordinator, Phase A Microbiology for Medical and Dental
Students, University of Florida, College of Medicine
1983 Dean's Task Force for program in Cell Biology, University of
Florida, College of Medicine
1986 Member Review Group for Curriculum for undergraduate students
enrolled in the College of Liberal Arts and Sciences, University of Florida
1988-2002 Coordinator, Immunology Segment of the Microbiology course for
2nd year Medical Students, Medical College of Ohio
1996 Member Analysis and Synthesis Working Group for Curriculum 2000
Medical College of Ohio
1997-1999 Member Curriculum Review Task Force, Medical College of Ohio
1997-1999 Member Curriculum Committee Years 1 and 2. Medical College of Ohio
1997-1999 Member Curriculum Implementation Group, Medical College of Ohio
1997-1998 Director Immunity and Infection Core for New Curriculum
1999 Co-organizer AMA workshop on Patient Oriented Problem Solving Packages
(POPS) to teach immunology
1999-2002 Co-editor POPS Educational Review Advisory Board
2008-2010 Center for Scholarship and Learning Center Advisory Board.
2012- External Advisor for Juniata College’s Howard Hughes Medical Institute
Undergraduate Award.
Teaching responsibilities
Michael D. Boyle
6
Lectures, small group exercises, problem-based learning and laboratory exercises for medical and
dental students in the areas of Immunology, host defense and Microbiology.
Lectures and nurses on topics related to immunology and host defense.
Undergraduate courses in microbiology, with laboratory, and immunology for majors.
Freshman seminar and scientific writing for majors.
Non-major courses on infectious disease and society and the ethical and societal implications of the
human genome project.
Graduate Education
Organizational activities
1981-1988 Full member University of Florida Graduate Faculty
1985-1988 Graduate Coordinator, Department of Immunology and
Medical Microbiology, University of Florida College of Medicine
1986 Judge for Medical guild Graduate Student Research Prize, University
of Florida College of Medicine
1986-1988 Member M.D.-Ph.D. Student Training Program, University of
Florida College of Medicine
1988-2002 Full member Graduate Faculty Medical College of Ohio
1988-2002 Coordinator, Immunology for Graduate Students, Medical College of Ohio
1993-2002 Member Graduate Executive Committee, Medical College of Ohio
1995-2002 Member M.D.-Ph.D. Committee, Medical College of Ohio
1996 Judge, MCO Graduate Student Forum
1996-2002 Member, Bioengineering Center, University of Toledo
1997-2002 Member of Molecular Basis of Disease Ph.D. Program
Teaching responsibilities
Lectures, small group exercises and laboratory rotations for graduate students on topics related to
biotechnology, immunology and host defense. Act as major advisor and serve on graduate student
committees.
Graduate Students (major advisor)
1981-1983 Kathleen J. Reis, Ph.D. dissertation title: "Identification of Bacterial
Fc Receptors and Characterization of a Group C Streptococcal Fc Receptor."
1982-1985 Ervin Faulmann, Ph.D. dissertation title: "Regulation of Serum Protease Activity:
Action of C1-inhibitor on the Activity of Serum C1 and Nerve Growth Factor."
1982-1985 Michele S. Yarnall, Ph.D. dissertation title: "Isolation and Characterization of
Group A Streptococcal Fc Receptors."
Michael D. Boyle
7
1983-1985 David Yarnall, MS thesis title: "The Role of C8 and C9 in Determining the
Range of Targets that can be Lysed by Complement."
1985-1989 L. Jeannine Brady, Ph.D. dissertation title: "Isolation and Characterization of
Bacterial IgA Fc Receptors."
1985-1989 Christopher C. Broder, Ph.D. dissertation title: "Characterization of
Bacterial Plasmin Receptors."
1988-1991 Ronald Otten, Ph.D. dissertation title: "Analysis of selective binding activities
of bacterial Fc binding proteins."
1990-1993 Hong Wang, Ph.D. dissertation title: "Plasmin receptors and the invasive
potential of bacteria."
1991-1995 Todd Pack, Ph.D. dissertation title: "Analysis of heterogeneity of bacterial
IgG3-binding proteins."
1992-1997 Roberta Raeder, Ph. D. dissertation title: "The role of surface proteins reactive with
IgG on the pathogenesis of group A streptococcal disease."
1993-1998 Sybil D'Costa, Ph.D. dissertation title: "Analysis of group A streptococcal plasmin-
binding proteins."
1994-1998 Zhu Qing Li, Ph.D. dissertation title: "Molecular mechanism and biological
significance of the interaction between group A streptococci and the human
plasmin(ogen) system."
1999-2002 Myrna Saouda, dissertation title "Analysis of the role of SpeB in group A
streptococcal infections.
1999-2002 Tara Smith, dissertation title "Global regulation of virulence factors in
Streptococcus pyogenes, group A."
2000-2001 Abbey Carr, M.S. thesis title "Analysis of the functional properties of the Oxygen-
stable hemolysin (SLS) of Streptococcus pyogenes."
Undergraduate research.
2002-2012 Mentored 30 undergraduates in research projects at Juniata College. 90% of
these students continued their careers in either graduate school or medical
school.
Michael D. Boyle
8
Research-related activities
Academic
1971-1974 Assistant Radiation Control Officer, Department of Tumor Immunology,
Chester Beatty Research Institute, Sutton, Surrey, England
1985-1986 Program Planning Committee for Health Center Academic Wing,
University of Florida, College of Medicine
1989-1992 Institutional Committee on the Care and Use of Laboratory Animals,
Medical College of Ohio
1990-1991 Search Committee for Director of Laboratory Animal Medicine,
Medical College of Ohio
1990-1992 Member Dean's Committee on Research Space, Medical College of Ohio
1994- present Member Patent Committee, Medical College of Ohio
1994- present Member, Space Utilization Committee, NW Ohio Technology Park
1996-1997 Chairman ad hoc Committee on Intellectual Property Policy, Medical
College of Ohio
1997-1999 Member Medical College of Ohio Research Strategic Planning Committee
1997- 1998 Member New Programs and Technology Subcommittee Medical College,
of Ohio
1999-2002 Medical College of Ohio Liaison to Edison Biotechnology Center
2000-2002 Medical College of Ohio representative on the Board of Regents task force
on Biotechnology
Academic – Industrial
1985 University of Florida Health Center representative to the Governor's Task Force
on Biotechnology
1985-1988 Department of Pediatrics representative to the University of Florida, College of
Medicine Advisory Committee on Biotechnology
1990 Testified to National Biotechnology Panel on funding for Academic
Biotechnology, September 1990
1999-2002 Member of the Board of Directors, Center for Technology
Commercialization, Toledo, Ohio
1999-2002 Member, Technology Advisory Group, Center for Technology
Commercialization, Toledo, Ohio
1999 Member Edison Incubator Director's Council
1999-2000 Member, National Business Incubator Association
1999-2002 Medical College of Ohio Coordinator for SBIR/STTR Grant Writing
Consortium [Director, Pat Snyder, BioStart, Cincinnati, OH]
Michael D. Boyle
9
Technology transfer activities
1986-1993 President and Founder of Gator Microbiologicals Inc. a biotechnology
company formed to transfer bacterial IgG-binding protein technology
from my academic laboratory to the market place.
Gator Microbiologicals was taken over byReceptorPro in 1993.
2009-present Member board and chairman of scientific advisory board of PEARL.
Consultant
1989-present Consultant to Blake Laboratories, Great Neck, New York
1993-2000 Consultant to ReceptorPro, Toledo, Ohio
1996 Consultant NW Ohio Venture Fund, Toledo, Ohio
1996-present Consultant - Morphogenesis, Alachua, Florida
2007-present Consultant – APD Life Sciences, State College, PA.
Patents
US Patent # Title Issue date
US patent #4,883,754 Bacterial Fc receptors Issued November 28, 1989
US patent #4,900,660 Streptococcal FcRc Issued February 13, 1990
US patent #4,945,157 Novel extraction procedure for
protein G Issued July 31, 1990
US patent #4,948,725 Type VI Fc receptors Issued August 19, 1990
US patent #4,977,082 Type VI Fc receptors Issued December 11, 1990
US patent #5,071,756 Bacterial Fc Receptors Issued December 10, 1991
US patent #5,085,894 Novel type VI bacterial Fc
receptors Issued February 4, 1992
US patent #5,082,931 Type II bacterial Fc receptors Issued January 21, 1992
US patent #5,098,827 Novel bacterial markers for
pathogenic group B streptococci Issued March 24, 1992
US patent #5,237,050 Bacterial plasmin receptors as
fibrinolytic agents Issued August 17, 1993
US patent #5,328,996 Bacterial plasmin receptors as
fibrinolytic agents Issued July 12, 1994
U.S. patent #5,472,696 Antigen of group B streptococci Issued December 5, 1995
U.S. patent #5,721,339 Gamma antigen of pathogenic
group B streptococci Issued February 24, 1998
U.S. patent #6,136,323
Bacterial plasmin receptors as
fibrinolytic agents Issued October 24, 2000
U.S. patent #61/283,417 General method for generating Pending
Michael D. Boyle
10
ultra-high affinity binding
proteins.
Funding History: (*indicates award involving industrial collaboration)
Date Agency Title Amount P.I.
1981-1982 National Institutes of
Health-BSRG
Antibody complement
killing of nucleated cells
$3,000 Michael D. P. Boyle
1982-1983 American Cancer
Society (ACS-82-
067)
Susceptibility of leukemic
cells to antibody and
complement
$3,500 Michael D. P. Boyle
1982-1983 Milheim Foundation
(09551-00)
Purging of tumor cells from
bone marrow
$16,399 Michael D. P. Boyle
1983-1985 American Heart
Association, Florida
Affiliate
Clq Receptors and
Pathogenesis of Bacterial
Endocarditis
$34,000 Michael D. P. Boyle
1983-1986 National Institutes of
Health
Role of Fc Reactive
Proteins in the Pathogenesis
of Poststreptococcal
Nephritis
$122,624 Michael D. P. Boyle
1983-1986 National Institutes of
Health
Elimination of Neuroblasts
from Bone Marrow with
Antibody and Complement
$180,377 Michael D. P. Boyle
*1986-1987 Cilco Activation of Complement
Pathways by Intraoccular
Lens
$5,500 Michael D. P. Boyle
1986-1989 National Institutes of
Health
Role of Fc Reactive
Proteins in the Pathogenesis
of Poststreptococcal
Nephritis
$242,106 Michael D. P. Boyle
1985-1988 National Science
Foundation
Use of Bacterial Fc Reactive
Proteins in
Immunotechnology
$237,000 Michael D. P. Boyle
1986-1988 American Heart
Association Florida
Affiliate
Role of Plasmin Receptors
in Bacterial Endocarditis
Program Project Grant with
Drs. Lottenberg, Gee, and
Shands
$228,391 Richard Lottenberg
Principal Investigator
Michael D. P. Boyle
Co-Prin. Investigator
1987-1990 National Institutes of
Health
Islet Cell Autoantibodies
and the Natural History of
IDD
$312,389 Noel McClaren
Principal
Investigator
Michael D. Boyle,
Co-prin. Investigator
*1989-1990 Blake Laboratories Isolation and
characterization of a group
B streptococcal IgA-binding
$37,500 Michael D. P. Boyle
Michael D. Boyle
11
protein
Michael D. Boyle
12
Date Agency Title Amount P.I.
*1989-1990 Blake Laboratories Cloning a Group B
streptococcal IgA-binding
protein
$37,500 Ervin Faulmann,
Principal
Investigator
Michael D. Boyle,
Co-prin. Investigator
1989-1992 National Institutes of
Health
Characterization of a
streptococcal plasmin
receptor
$427,254
Subcon.
$100,227
Richard Lottenberg,
Principal
Investigator
Michael D. Boyle,
Co-prin. Investigator
1991-1993 American Heart
Association Ohio
Affiliate
Use of bacterial plasmin
receptor - plasmin
complexes as plasminogen
activators
$18,743 Michael D. P. Boyle
1992-1993 National Institutes of
Health-BSRG
Role of type II IgG-
binding proteins in group
A streptococcal infections
$7,850 Michael D. P. Boyle
*1993 AMVAX, Inc. Isolation of a non-IgA-
binding form of the group
B
streptococcal beta antigen
$1,250 Michael D. P. Boyle
*1993-1995 Ohio Edison
Biotechnology Center,
Biomedical
Partnership Grant
Production of
streptococcal protein G
$66,600 Michael D. P. Boyle
*1994-1995 Blake Laboratories Preparation of
recombinant protein B
$2,750 Michael D. P. Boyle
*1994-1995 National Institutes of
Health - STTR
Bacterial binding proteins
for human IgM
$100,000 Michael D. P. Boyle
1992-1998 National Institutes of
Health
Characterization of a
streptococcal plasmin
receptor
$628,300 Richard Lottenberg,
Principal
Investigator Michael
D. P. Boyle, Co-Prin.
Investigator
1996-1999 American Heart
Association (Ohio
Affiliate)
Influence of cytokines on
group A streptococcal
infections
$90,000 Dennis W. Metzger,
Principal
Investigator Michael
D. P. Boyle, Co-Prin.
Investigator
1993-1999
National Institutes of
Health
Human IgG subclass-
specific bacterial binding
proteins
$525,130 Michael D. P. Boyle
Michael D. Boyle
13
Date Agency Title Amount P.I.
1996-1999 National Institutes of
Health STTR program
Phase II
Bacterial binding proteins
for human IgM
$495,000 Michael D. P. Boyle
In collaboration with
ReceptorPro.
1998-2000 American Medical
Association
Patient-Oriented Problem
Solving (POPS) system
for teaching immunology
$48,000 P. Small, M.D.
Principal
Investigator
Michael D. P. Boyle
Co-Investigator
1999-2000 Ohio Department of
Development
Life Sciences Incubator
Feasibility Study
$39,000 Michael D. P. Boyle
1998-2003 National Institutes of
Health
Interaction of group A
streptococci with human
plasminogen system
$587,348 Michael D. P. Boyle
2002-2005 National Institutes of
Health (RO1)
Immunoproteomics $450,000 Michael D. P. Boyle,
*2002-
2004
National Institutes of
Health STTR Program
Identification of a bacterial
IgE-binding protein
$180,000
(sub-
contract)
In collaborator with
Biocheck Labs,
Toledo Ohio
*2002-
2004
Life Sciences
Greenhouse of
Central Pennsylvania
Identification of a bacterial
IgY-binding protein
$71,000
In collaborator with
Biocheck Labs,
Toledo Ohio
2003-2005 National Institutes of
Health (R03)
Immunoproteomics and
detection of viral disease
$100,000
In collaboration with
Dr. Mark Buller, St
Louis University
2004 Center for
Educational
Technology
Implications of the NIH
Roadmap for
Undergraduate Life
Sciences Education
$14,734 Michael D. P. Boyle,
*2005 Life Sciences
Greenhouse of
Central Pennsylvania
Business Opportunity
Validation Grants
$25,000
In collaborator with
Biocheck Labs,
Toledo Ohio
*2005 National Institutes of
Health/SBIR
Low-cost Affinity system
for Preparation of
plasma/serum for
Biomarker Analysis
$41,576 In collaborator with
APDLS, State
College, PA.
2005-2007 American Heart
Association)
Characterization of a
Putative Strepcoagulase
$120,000
Michael D. P. Boyle
2007-2010 National Institutes of
Health (R21)
Characterization of
streptococcal IDES
$150,000 Michael D. P. Boyle
*2010 PEARL Biosciences
State College PA.
Genetically engineer high
affinity IgG binding protein
$15,000 Michael D. P. Boyle
Michael D. Boyle
14
*2011-
2012
PEARL Biosciences
State College PA.
IgA binding protein $23,765 Michael D. P. Boyle
2011-2012 National Science
Foundation
RCN UBE Transforming
Undergraduate Education
through Increased Faculty
Access to NextGen
Sequencing Runs
$50,000 Michael D. P. Boyle
BIBLIOGRAPHY
Michael D. P. Boyle
Peer-reviewed articles
1. Boyle, M.D.P. and Ormerod, M.G. (1975) The destruction of tumor cells by alloimmune
peritoneal cells: Mechanism of action of activated macrophages in vitro. J.
Reticuloendothel. Soc. 17:73-83.
2. Boyle, M.D.P. and Ormerod, M.G. (1975) The destruction of allogeneic tumor cells by
peritoneal macrophages from immune mice: Purification of lytic effectors. Cell. Immunol.
13:247-258.
3. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1975) Lysis of tumor cells by antibody and
complement. III. Lack of correlation between antigen movement and cell lysis. J.
Immunol. 115:473-475.
4. Boyle, M.D.P. and Ormerod, M.G. (1976) Destruction of allogenic tumor cells by
peritoneal macrophages: Production of lytic effectors by immune mice. Transplantation
21:242-246.
5. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1976) Lysis of tumor cells by antibody and
complement. VI. Enhanced killing of enzyme pretreated tumor cells. J. Immunol.
116:661-668.
6. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1976) Detection of complement-dependent
antibody to tumor cells in sera of strain-2 guinea pigs cured of their tumors by BCG
treatment. J. Natl. Can. Inst. 56:623-626.
7. Jolley, G.M., Boyle, M.D.P. and Ormerod, M.G. (1976) The destruction of allogenic
tumor cells by antibody and adherent cells from peritoneal cavities of mice. Cell.
Immunol. 22:262-270.
Michael D. Boyle
15
8. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1976) Studies on the terminal stages of
antibody-complement mediated killing of a tumor cell. I. Evidence for the existence of an
intermediate T*. J. Immunol. 116:1272-1275.
9. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1976) Studies on the terminal stages of
antibody-complement mediated killing of a tumor cell. II. Inhibition of transformation of
T* to dead cells by 3'5' cAMP. J. Immunol. 116:1276-1279.
10. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1976) Studies on the terminal stages of
antibody-complement mediated killing of a tumor cell. III. Effect of membrane active
agents. J. Immunol. 117:106-109.
11. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1976) Lysis of tumor cells by antibody and
complement. VII. Complement-dependent 86Rb release--A non-lethal event? J. Immunol.
117:1346-1350.
12. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1977) Effect of Concanavalin A on the
function activity of hemolytic antibody. Immunol. Commun. 6:283-296.
13. Boyle, M.D.P. and Ohanian, S.H. (1977) Quantitation of antibody-complement mediated
lysis of tumor cells. J. Immunol. Methods. 15:135-146.
14. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1977) Effect of Concanavalin A on the
classical complement pathway. J. Immunol. 118:1622-1625.
15. Boyle, M.D.P., Langone, J.J., Ohanian, S.H. and Borsos, T. (1977) Effect of
Concanavalin A on the killing of tumor cells by antibody and complement. J. Immunol.
118:1626-1630.
16.* Schlager, S.I., Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1977) Effect of inhibiting
DNA, RNA, and protein synthesis of tumor cells on their susceptibility to killing by
antibody and complement. Cancer Res. 37:1432-1437.
17. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1977) 125 I Protein A: Applications to the
quantitative determination of fluid phase and cell-bound IgG. J. Immunol. Methods.
28:281-293.
18. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1978) Complement-dependent cytotoxic
antitumor antibody. I. Immunoglobulin class determined by interaction with Protein A or
Concanavalin A. J. Natl. Can. Inst. 60:411-418.
19. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1978) Effect of protease treatment on the
sensitivity of tumor cells to antibody-GPC killing. Clin. Immunol. Immunopathol. 10:84-
94.
Michael D. Boyle
16
20. Boyle, M.D.P., Langone, J.J. and Borsos, T. (1978) Studies on the terminal stages of
immune hemolysis. III. Distinction between the insertion of C9 and the formation of a
transmembrane channel. J. Immunol. 120:1721-1725.
21. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1978) Studies on the interaction between
Protein A and immunoglobulin G. I. Effect of Protein A on the functional activity of IgG.
J. Immunol. 121:327-332.
22. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1978) Studies on the interaction between
Protein A and immunoglobulin G. II. Composition and activity of complexes formed
between Protein A and IgG. J. Immunol. 121:333-338.
23. Boyle, M.D.P., Langone, J.J. and Borsos, T. (1978) Effect of lectins on the hemolytic
activity of complement components. Immunochemistry 15:465-470.
24. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1979) A solid phase immunoassay for
human immunoglobulin E. Use of 125I Protein A as the tracer. Analyt. Biochem. 93:207-
215.
25. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1979) Antigenic distinction between human
C9 and guinea pig C9. Clin. Immunol. Immunopathol. 13:103-113.
26. Boyle, M.D.P., Langone, J.J. and Borsos, T. (1979) Studies on the terminal stages of
immune hemolysis. IV. Effect of metal salts. J. Immunol. 122:1209-1213.
27. Lo, T.N. and Boyle, M.D.P. (1979) Relationship between the intracellular cyclic
adenosine 3':5'-monophosphate level of tumor cells and their sensitivity to killing by
antibody and complement. Cancer Res. 39:3158-3162.
28. Boyle, M.D.P., Langone, J.J. and Borsos, T. (1979) 1,10 Phenanthroline mediated
hemolysis--Evidence that 1,10 phenanthroline does not substitute specifically for C9.
Molec. Immunol. 16:307-312.
29. Boyle, M.D.P. and Borsos, T. (1979) Studies on the terminal stages of immune hemolysis.
V. Evidence that not all complement-produced transmembrane channels are equal. J.
Immunol. 122:71-76.
30. Boyle, M.D.P. and Langone, J.J. (1979) Complement-dependent cytotoxic antitumor
antibody. II. Quantitative determination of cell bound IgM. J. Natl. Can. Inst. 62:1537-
1544.
31. Boyle, M.D.P., Gee, A.P. and Borsos T. (1979) Studies on the terminal stages of immune
hemolysis. VI. Osmotic blockers of differing Stokes' radii detect complement-induced
transmembrane channels of differing size. J. Immunol. 122:77-82.
Michael D. Boyle
17
32. Gee, A.P., Borsos, T. and Boyle, M.D.P. (1979) Interaction between components of the
human classical complement pathway and immobilized Cibacron Blue F3GA. J. Immunol.
Methods. 30:119-126.
33. Boyle, M.D.P. and Langone, J.J. (1980) A simple procedure to use whole serum as a
source of either IgG or IgM specific antibody. J. Immunol. Methods. 32:51-58.
34. Boyle, M.D.P. and Borsos, T. (1980) The terminal stages of immune hemolysis-A brief
review. Molec. Immunol. 17:425-432.
35. Gee, A.P., Boyle, M.D.P. and Langone, J.J. (1980) Effect of Concanavalin A on the
hemolytic activity of the components of the classical complement pathway. Molec.
Immunol. 17:143-146.
36. Boyle, M.D.P. and Ohanian, S.H. (1980) Evidence for the influence of the initial
complement components on the assembly and activity of the membrane attack complex. J.
Immunol. 124:2824-2827.
37. Gee, A.P., Boyle, M.D.P. and Borsos, T. (1980) Distinction between C8-mediated and
C8/C9-mediated hemolysis on the basis of independent 86Rb and hemoglobin release. J.
Immunol. 124:1905-1910.
38. Okada, M., Boyle, M.D.P. and Borsos, T. (1980) Evidence for a temperature-dependent
and temperature-independent pathway in the generation of complement-mediated
transmembrane channels. Biochem. Biophys. Res. Commun. 94:406-412.
39. DeLisi, C., Boyle, M.D.P. and Borsos, T. (1980) Analysis of the colloid osmotic step of
complement-mediated immune hemolysis. J. Immunol. 125:2055-2062.
40. DeLisi, C., Boyle, M.D.P. and Borsos, T. (1980) Mathematical analysis of the reaction of
EAC1-8 with C9: Identification of parameters defining conditions for molecular titration.
J. Immunol. 125:2334-2338.
41. Boyle, M.D.P., Gee, A.P., Okada, M. and Borsos, T. (1980) Differences in cell-bound C8
sites on chicken erythrocytes measured by their reactivity with guinea pig and human C9.
J. Immunol. 125:2818-2822.
42. Boyle, M.D.P. (1981) Inhibition by zinc of the binding of C1 to antigen-antibody
complexes. Biochem. Biophys. Res. Commun. 103:856-862.
43. Boyle, M.D.P., Gee, A.P. and Borsos, T. (1981) Heterogeneity in the size and stability of
transmembrane channels produced by whole complement. Clin. Immunol. Immunopathol.
20:287-295.
Michael D. Boyle
18
44. Gee, A.P., Boyle, M.D.P. and Borsos, T. (1981) Differences in the functional activity of
human and guinea pig C1 based on their reactivity with IgG at low temperatures.
Molecular Immunology 18:609-613.
45. Boyle, M.D.P. and Young, M. (1982) Nerve growth factor: Activation of the classical
complement pathway by specific substitution for component C1. Proc. Natl. Acad. Sci.
USA 79:2519-2522.
46. Reis, K.J., Ayoub, E.M. and Boyle, M.D.P. (1983) Detection of receptors for the Fc
region of IgG on Streptococci. J. Immunol. Methods 59:83-94.
47. Gee, A.P., Boyle, M.D.P., Lawman, M.J. P., Munger, K.L. and Young M. (1983) Nerve
growth factor: Stimulation of polymorphonuclear leukocyte chemotaxis in vitro. Proc.
Natl. Acad. Sci. 80:7215-7218.
48. Barrett, D.J. and Boyle, M.D.P. (1984) Restoration of complement function in vitro by
plasma infusion in factor I (C3b inactivator) deficiency. J. Pediatr. 104:76-81.
49. Reis, K.J., Boyle, M.D.P., and Ayoub, E.M. (1984) Identification of distinct Fc-receptor
molecules on streptococci and staphylococci. J. Clin. Lab. immunol. 13:75-80.
50. Boyle, M.D.P. (1984) Is the membrane attack complex of complement an enzyme? Mol.
Cell. Biochem. 61:5-1553.
51. Reis, K.J., Ayoub, E.M., and Boyle, M.D.P. (1984) Streptococcal Fc receptors: I.
Isolation and partial characterization of the receptor from a group C streptococcus. J.
Immunol. 132:3091-3097.
52. Reis, K.J., Ayoub, E.M., and Boyle, M.D.P. (1984) Streptococcal Fc receptors: II.
Comparison of the reactivity of a receptor from a group C streptococcus with
staphylococcal protein A. J. Immunol. 132:3098-3102.
53. Lawman, M.J.P., Thurmond, M.C., Reis, K.J., Gauntlett, D.R., and Boyle, M.D.P. (1984)
Solid phase radioimmunoassay for the detection of immunoglobulins against bovine
Brucella abortus. Vet. Immunol. Immunopathol. 6:291-305.
54. Lawman, M.J.P., Boyle, M.D.P., Gee, A.P., and Young, M. (1984) A rapid technique for
measuring leukocyte chemotaxis in vitro. J. Immunol. Methods 69:197-206.
55. Reis, K.J., Yarnall, M., Ayoub, E.M. and Boyle, M.D.P. (1984) Effect of mouse passage
on Fc receptor expression by group A streptococci. Scand. J. Immunol. 20:433-439.
56. Boyle, M.D.P. (1984) Applications of bacterial Fc receptors in immunotechnology.
Biotechniques 2:334-340.
Michael D. Boyle
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57. Gee, A.P., van Hilten, J., Graham-Pole, J., and Boyle, M.D.P. (1985) Antibody
complement killing of neuroblasts. Progress in Clinical and Biological Research 175:471-
483.
58. Boyle, M.D.P., Lawman, M.J.P., Gee, A.P., and Young, M. (1985) Nerve growth factor:
A chemotactic factor for polymorphonuclear leukocytes in vitro. J. Immunol. 134:564-568.
59. Yarnall, M., Reis, K.J., Ayoub, E.M., and Boyle, M.D.P. (1985) An immunoblotting
technique for the detection of bound and secreted bacterial Fc receptors. J. Microbiol.
Methods. 3:83-93.
60. Gee, A.P., Rolfe, A.E., Worthington-White, D., Graham-Pole, J., and Boyle, M.D.P.
(1985) A rapid alternative to the clonogenic assay for measuring antibody and complement
mediated killing of tumor cells. Clin. Immunol. Immunopathol. 34:263-271.
61. Young, M., Gee, A.P., Munger, K.L., Lawman, M.J.P., and Boyle, M.D.P. (1985)
Leukocyte chemotaxis mediated by nerve growth factor. A non-proteolytic function of the
proteolytic active site. Mol. Cell. Biochem. 66:65-69.
62. Gee, A.P., Bruce, K.M., Morris, T.M. and Boyle, M.D.P. (1985) Evidence for an anti-
complementary factor associated with human bone marrow cells. J. Natl. Cancer Inst.
75:441-445.
63. Reis, K.J., Ayoub, E.M. and Boyle, M.D.P. (1985) A rapid method for the isolation and
characterization of a homogeneous population of streptococcal Fc receptors. J. Microbiol.
Methods 4:45-58.
64. Boyle, M.D.P., Wallner, W.A., von Mering, G.O., Reis, K.J., and Lawman, M.J.P. (1985)
Interaction of bacterial Fc receptors with goat immunoglobulin. Mol. Immunol. 22:1115-1121.
65. Lawman, M.J.P., Boyle, M.D.P., Gee, A.P. and Young, M. (1985) Nerve growth factor
accelerates the early cellular events associated with wound healing. Exp. Mol. Pathol.
43:274-281.
66. Lawman, M.D.P., Joiner, S., Gauntlett, D.R., and Boyle, M.D.P. (1985) A rapid
purification for bovine IgG2. Evidence for IgG2a and IgG2b sub-classes. Compar.
Immunol. Microbiol. and Infect. Dis. 8:1-8.
67. Yarnall, M., and Boyle, M.D.P. (1986) Identification of a unique receptor on a group A
streptococcus for the Fc region of human IgG3. J. Immunol. 136:2670-2673.
68. Yarnall, M., and Boyle, M.D.P. (1986) Influence of Dipeptides on Interaction of
Immunoglobulins with Bacterial Fc Receptors. Biochem. Biophys. Res. Comm. 135:1105-
1111.
Michael D. Boyle
20
69. Yarnall, M. and Boyle, M.D.P. (1986) Isolation and partial characterization of a type II Fc
receptor from a group A streptococcus. Mol. and Cell Biochem. 70:57-66.
70. Lawman, M.J.P., Ball, D.R., Hoffman, E.M., Des Jardin, L.E., and Boyle, M.D.P. (1986)
Production of Brucella abortus specific protein A reactive antibodies (IgG2) in infected and
vaccinated cattle. Vet. Microbiol. 12:43-53.
71. Yarnall, M., Ayoub, E.M., and Boyle, M.D.P. (1986) Analysis of Surface Receptor
Expression on Bacteria Isolated from Patients with Endocarditis. J. Gen. Microbiology
132:2049-2051.
72. von Mering, G.O. and Boyle, M.D.P. (1986) Comparison of type III Fc receptors
associated with group C or group G streptococci. Mol. Immunol. 23:811-821.
73. Yarnall, D.P. and Boyle, M.D.P. (1986) Restriction in the Lytic Efficiency of Complement
on Different Erythrocyte Targets: A Re-examination of the Activities of Horse C8 and C9.
Mol. Immunol., 23:823-829.
74. Yarnall, M., and Boyle, M.D.P. (1986) Isolation and Characterization of Type IIa and
Type IIb Streptococcal Fc Receptors. Scand J. Immunol. 24:549-557.
75. Gee, A.P., Bruce, K.M., van Hilten, J., Siden, E.J., Braylan, R.C., Bauer, P.C., and Boyle,
M.D.P. (1987) Selective Loss of Expression of a cALLa Associated antigen on a Human
Leukemic Cell Line Induced by Treatment with Monoclonal Antibody and Complement. J.
Natl. Cancer Inst. 78:29-35.
76. Boyle, M.D.P. and Gee, A.P. (1987) Low antigen density tumor cells - an obstacle to
effective autologous bone marrow purging? Cancer Invest. 5:113-118.
77. Faulmann, E., Young, M., and Boyle, M.D.P. (1987) Interaction of nerve growth factor
and C1 inhibitor. J. Immunol. 138:4336-4340.
78. Faulmann, E., and Boyle, M.D.P. (1987) Regulation of activation of C1 in serum.
Molecular Immunol. 24:655-660.
79. Boyle, M.D.P., Chiodo, V.A., Lawman, M.J.P., Gee, A.P., and Young, M. (1987)
Urokinase: A chemotactic factor for polymorphonuclear leukocytes in vitro. J. Immunol.
139:169-174.
80. Boyle, M.D.P. and Reis, K.J. (1987) Bacterial Fc receptors. Biotechnology 5:697-703.
81. Lottenberg, R., Broder, C., and Boyle, M.D.P. (1987) Identification of a specific receptor
for plasmin on a group A streptococcus. Infect. Immun. 55:1914-1928.
Michael D. Boyle
21
82. Wallner, W.A., Lawman, M.J.P., and Boyle, M.D.P. (1987) Reactivity of bacterial Fc
receptors with bovine immunoglobulins: Implications and limitations for quantitative
immunochemistry. Appl. Microbiol. Biotechnol. 27:168-173.
83. Reis, K.J., Siden, E.J., and Boyle, M.D.P. (1988) Selective Colony Blotting to Expand
Bacterial Surface Receptors: Applications to Receptors for Rat Immunoglobulins.
Biotechniques 6:130-136.
84. Gee, A.P. and Boyle M.D.P. (1988) Purging Tumor Cells From Bone Marrow by Use of
Antibody and Complement: A Critical Appraisal. J. Natl. Cancer Inst. 80:154-159.
85. Reis, K.J., von Mering, G.O., Karis, M.A., Faulmann, E.L., Lottenberg, R., and Boyle,
M.D.P. (1988) Enzyme-labeled type III bacterial Fc receptors - a versatile tracer for
immunoassay. J. Immunol. Methods 107:273-280.
86. Feher, S.L., Boyle, M.D.P., and Halliwell, R.E.W. (1988) Identification of protein A from
Staphylococcus intermedius isolated from canine skin. Am. J. Vet. Res. 49:697-701.
87. Broeseker, T.A., Boyle, M.D.P., and Lottenberg, R. (1988) Characterization of the
interaction of human plasmin with its specific receptor on a group A streptococcus.
Microbial Pathogenesis 5:19-27.
88. Brady, L.J., Daphtary, U.D., Ayoub, E.M., and Boyle, M.D.P. (1988) Two novel antigens
associated with group B streptococci identified by a rapid two stage radioimmunoassay. J.
Infect. Dis. 158:965-972.
89. Young, M., Blanchard, M.H., Sessions, F., and Boyle, M.D.P. (1988) The subunit structure
of high molecular weight mouse nerve growth factor. Biochemistry 27:6675-6681.
90. DesJardin, L.E., Lottenberg, R., and Boyle, M.D.P. (1989) Group A streptococci bind
human plasmin but not other structurally related proteins. Thrombosis Res. 55:187-193.
91. Brady, L.J., and Boyle, M.D.P. (1989) Identification of non-immunoglobulin A-Fc-
binding forms and low-molecular-weight secreted forms of the group B streptococ
antigen. Infect. Immun. 57:1573-1581.
92. Broder, C.C., Lottenberg, R., and Boyle, M.D.P. (1989) Mapping of the domain of human
plasmin recognized by its unique group A streptococcal receptor. Infect. Immun. 57:2597-
2605.
93. Faulmann, E.L., Otten, R.A., Barrett, D.J., and Boyle, M.D.P. (1989) Immunological
Applications of Type III Fc Binding Proteins: Comparison of Different Sources of Protein
G. J. Immunological Methods 123:269-281.
Michael D. Boyle
22
94. Coleman, S.E., Brady, L.J., and Boyle, M.D.P. (1990) Colloidal gold immunolabelling of
Immun., 58:332-340.
95. Heath, D.G., Boyle, M.D.P. and Cleary, P.P. (1990) Isolated DNA repeat region from
fcrA76, the Fc-binding protein gene from an M-type 76 strain of group A streptococci,
encodes a protein with Fc-binding activity. Mol Microbiol., 4:2071-2079.
96. Raeder, R., Otten, R.A., and Boyle, M.D.P. (1991) Isolation and partial characterization of
a type IV bacterial immunoglobulin binding protein. Mol Immunol., 28:661-671.
97. Raeder, R., Faulmann, E.L., and Boyle, M.D.P. (1991) Evidence for functional
heterogeneity in IgG Fc binding proteins associated with group A streptococci. J.
Immunol., 146:1247-1253.
98. Chun, C.Y., Brady, L. J., Boyle, M.D.P., Dillon, H. C., and Ayoub, E. M. (1991) Group B
streptococcal C protein-associated antigens: Association with neonatal sepsis. J. Infect.
Dis.,163:786-791.
99. Raeder, R., Otten, R.A., and Boyle, M.D.P. (1991) Comparison of albumin receptors
expressed on bovine and human group G streptococci Infect. Immun., 59:609-616.
100. Broder, C.C., Lottenberg, R., von Mering, G.O., Johnston, K.H., and Boyle, M.D.P. (1991)
Isolation of a prokaryotic plasmin receptor: Relationship to a plasminogen activator
produced by the same micro-organism. J. Biol. Chem. 266:4922-4928.
101. Faulmann, E.L., and Boyle, M.D.P. (1991) A simple preparative procedure to extract and
purify protein G from group G streptococci. Preparative Biochem. 21:75-86.
102. Faulmann, E. L., Duvall, J. L., and Boyle, M. D. P. (1991) Protein B: A versatile bacterial
Fc-binding protein selective for human IgA, Biotechniques 10:748-755.
103. Raeder, R. A. and Boyle, M. D. P., (1991) Detection of rheumatoid-like factors in serum of
chickens immunized with bacterial immunoglobulin binding proteins. J. Immunol. Methods
138:201-209.
104. Otten, R. A. and Boyle, M. D. P. (1991) The mitogenic activity of type III bacterial
immunoglobulin binding proteins (protein G) for human peripheral blood lymphocytes is
not related to their ability to react with human serum albumin or IgG , J. Immunol.
146:2588-2595.
105. Otten, R. A. and Boyle, M. D. (1991) Characterization of protein G expressed by human
group C and G streptococci, J. Microbiol. Methods 13:185-200.
106. Otten, R. A., Raeder, R. A., Heath, D. G., Lottenberg, R., Cleary, P. P., and Boyle,
Michael D. Boyle
23
M. D. P. (1992) Identification of two type IIa IgG-binding proteins expressed by a single
group A streptococcus. J. Immunol. 148:3174-3182..
107. Lottenberg, R., DesJardin, L. E., Wang, H. and Boyle, M.D.P. (1992) Streptokinase
producing streptococci grown in human plasma acquire unregulated cell-associated plasmin
activity. J. Infect. Dis. 166:436-440.
108. Lottenberg, R., Broder, C.C., Boyle, M.D.P., Kain, S. J., Schroeder, B.L. and Curtiss, R.
(1992) Cloning, sequence analysis, and expression in Escherichia coli of a streptococcal
plasmin receptor. J. Bact. 174:5204-5210.
109. Raeder, R., Otten, R., Chamberlin, L., and Boyle, M.D.P. (1992) Functional and
serological analysis of type II IgG-binding proteins expressed by pathogenic group A
streptococci. J. Clin. Microbiol., 30:3074-3081.
110. Raeder, R. and Boyle, M.D.P. (1993) Association between expression of immunoglobulin
G-binding proteins by group A streptococci and virulence in a mouse skin infection model.
Infect. Immun., 61:1378-1384.
111. Boyle, M.D.P. and Raeder, R. (1993) Analysis of heterogeneity of IgG-binding proteins
expressed by group A streptococci. ImmunoMethods, 2:41-55.
112. Raeder, R. and Boyle, M.D.P. (1993) Association of type II IgG-binding protein
expression and survival of group A streptococci in human blood. Infect. Immun., 61:3696-
3702.
113. Boyle, M.D.P., Faulmann, E.L., and Metzger, D.W. (1993) Use of Antibodies for Analysis
of Bacterial Proteins, J. Microbiol. Methods, 18:289-303.
114. Boyle, M.D.P. (1993) Analysis of host-bacterial interactions - implications for
pathogenesis, J. Microbiol. Methods, 18:163-165.
115. Wang, H., Lottenberg, R. and Boyle, M.D.P. (1994) Analysis of plasmin (ogen)
acquisition by clinical isolates for group A streptococci incubated in human plasma. J.
Infect. Dis. 169:143-149.
116. Lottenberg, R., Minning-Wenz, D. and Boyle, M.D.P. (1994) Capturing host plasmin
(ogen): a common mechanism for invasive pathogens? Trends in Microbiol. 2:20-24.
117. Boyle, M.D.P., Hawlitzky, J. Raeder, R. and Podbielski, A. (1994) Analysis of the genes
encoding two unique type IIa IgG-binding proteins expressed by a single group A
streptococcus. Infect. Immun, 62:1336-1347.
Michael D. Boyle
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118. Pack, T.D., Otten, R.A., Raeder, R.H. and Boyle, M.D.P. (1994) Identification of two
functional forms of IgG3-binding protein expressed by group A streptococci. Infect.
Immun, 62:2104-2107.
119. Podbielski, A., Hawlitzky, J., Pack, T.D., Flosdorff, A. and Boyle, M.D.P. (1994) A group
A streptococcal Enn protein potentially resulting from intergenomic recombination exhibits
atypical immunoglobulin-binding characteristics. Mol. Microbiol., 12:725-736.
120. Wang, H., Lottenberg, R. and Boyle, M.D.P. (1995) A role for fibrinogen in the
streptokinase-dependent acquisition of plasmin(ogen) by group A streptococci J. Infect.
Dis. 171:85-92.
121. Boyle, M.D.P. (1995) Variation of multifunctional surface binding proteins - a virulence
strategy for group A streptococci? J. Theoretical Biol., 173:415-426.
122. Boyle, M.D.P., Weber-Heynemann, J., Raeder, R. and Podbielski, A. (1995) Characterization of
a gene coding for a type IIo bacterial IgG-binding protein. Mol. Immunol., 32:669-678.
123. Wang, H., Lottenberg, R. and Boyle, M.D.P. (1995) Analysis of the Interaction of Group
A Streptococci with Fibrinogen, Streptokinase and Plasminogen. Microbial Pathogen.,
78:153-166.
124. Raeder, R. and Boyle, M.D.P. (1995) Analysis of IgG-binding expression by invasive
isolates of Streptococcus pyogenes. Clin. Diagnos. Lab. Immunol., 2:484-486.
125. Raeder, R. and Boyle, M.D.P. (1995) Distinct profiles of IgG-binding protein expression
by invasive M1 serotype isolates of Streptococcus pyogenes. Clin. Diagnos. Lab.
Immunol., 2:478-483.
126. Metzger, D.W., Raeder, R., Van Cleave, V.H. and Boyle, M.D.P. (1995) Protection of
mice from group A streptococcal skin infection by interleukin 12. J. Infect. Dis., 171:1643-
1645.
127. Pack, T.D. and Boyle, M.D.P. (1995) Characterization of a type II'o group A streptococcal
immunoglobulin-binding protein. Mol. Immunol., 32:1235-1243.
128. Podbielski, A., Schnitzler, N., Beyhs, P. and Boyle, M.D.P. (1996) M-related protein
(Mrp) contributes to group A streptococcal resistance to phagocytosis by human
granulocytes. Mol. Microbiol., 19:429-441.
129. Christner, R. and Boyle, M.D.P. (1996) Role of staphylokinase in the acquisition of
plasmin(ogen)-dependent enzymatic activity by staphylococci. J. Infect. Dis., 173:104-
112.
Michael D. Boyle
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130. Pack, T.D., Podbielski, A., and Boyle, M.D.P. (1996) Identification of an amino acid signature sequence predictive of protein G-inhibitable IgG3-binding activity in group A streptococcal IgG-binding proteins. Gene, 171:65-70.
131. Raeder, R. and Boyle, M.D.P. (1996) Properties of IgG-binding proteins expressed by
Streptococcus pyogenes isolates are predictive of invasive potential. J. Infect. Dis.,
173:888-895.
132. Krebs, B., Kaufhold, A., Boyle, M.D.P. and Podbielski, A. (1996) Different alleles of the
fcrA/mrp gene of Streptococcus pyogenes encode M-related proteins exhibiting an identical
immunoglobulin-binding pattern. Med. Microbiol. Immunol., 185:39-47.
133. Tsivitse, M. and Boyle, M.D.P. (1996) Identification of distinct IgG-subclass binding
domains within a group A streptococcal type IIo IgG-binding protein. Can. J. Microbiol.,
42:1172-1175.
134. Christner, R., Li, Z., Raeder, R., Podbielski, A. and Boyle, M.D.P. (1997) Identification of
key gene products required for acquisition of plasmin-like enzymatic activity by group A
streptococci. J. Infect. Dis., 175:1115-1120.
135. Boyle, M.D.P. and Lottenberg, R. (1997) Plasminogen Activation by Invasive Human
Pathogens. Thrombosis Haemostasis, 77:1-10.
136. Talanin, N.Y., Shelley, W.B., Raeder, R., Shelley, E.D. and Boyle, M.D.P. (1997)
Detection of streptococcal class I M protein in psoriasis by confocal immunofluorescent
microscopy. Acta Dermator-Venereologica, 77:175-180.
137. D’Costa, S., Wang, H., Metzger, D. and Boyle, M.D.P. (1997) Group A streptococcal
isolate 64/14 expresses surface plasmin-binding structures in addition to Plr. Res. in
Microbiol., 148:559-572.
138. Schmidt, K-H., Podbielski, A., Raeder, R. and Boyle, M.D.P. (1997) Inactivation of single
genes within the virulence regulon of an M2 group A streptococcal isolate in differences in
virulence for chicken embryos and for mice. Mol. Pathogen., 23:347-355.
139. Li, Z., Gutierrez, J. A., Bleiweis, A.S., and Boyle, M.D.P. (1997) Efficient Insertional
Mutagenesis In Group A Streptococci Mediated By Tn917 Transposon. J. Micro. Methods.,
30:205-215.
140. Boyle, M.D.P., Raeder, R., Flosdorff, A., and Podbielski, A. (1998) Role of emm and mrp
genes in the virulence of group A streptococcal isolate 64/14 in a mouse model of skin
infection. J. Infect. Dis., 177:991-997.
141. Boyle, M.D.P., Vogel, L., Sisson, S., and Raeder. R. (1998) Do chickens immunized with
bacterial IgG-binding proteins produce rheumatoid factor-like antibodies? Scand. J. of
Immunol., 47:218-222.
Michael D. Boyle
26
142. Donabedian, H., and Boyle, M.D.P. (1998) Clot formation by group A streptococci.
Infect. Immun., 60:2362-2364.
143. D'Costa, S., and Boyle, M.D.P. (1998) Interaction of a group A streptococcus within
human plasma results in assembly of a surface plasminogen activator that contributes to
occupancy of surface plasmin-binding structures. Microb. Path., 24:341-349.
144. Raeder, R., Woischnik, M., Podbielski, A., and Boyle, M.D.P. (1998) A secreted
streptococcal cysteine protease can cleave a surface expressed M1 protein and alter the
immunoglobulin-binding properties. Res. Microbiol., 149:539-548.
145. Li, Z., Ploplis, V., French, E.L., and Boyle, M.D.P. (1999) Interaction between group A
streptococci and the plasmin(ogen) system promotes virulence in a mouse skin infection
model. J. Infect. Dis., 179:907-914.
146. Li, Z., Sledjeski, D., Kreikemeyer, B., Podbielski, A. and Boyle, M.D.P. (1999)
Identification of pel, a S. pyogenes locus that affects both surface and secreted proteins. J.
Bacteriol., 181:6019-6027.
147. Schroeder, B., Boyle, M.D.P., Sheerin, B.R., Asbury, A.C. and Lottenberg, R. (1999)
Species-specificity of plasminogen activation and the acquisition of surface-associated
proteolytic activity by group C streptococci grown in plasma. Infect. Immun. 67:6487-
6495.
148. Raeder, R., Barker-Merrill, L., Lester, T., Boyle, M.D.P. and Metzger, D.W. (2000) A
pivotal role for interferon-gamma in protection against group A streptococcal skin
infection. J. Infect. Dis., 181:639-645.
149. Raeder, R., Horokopathis, E., Hollingshead, S., and Boyle, M.D.P. (2000) Absence of
SpeB Production in Virulent Large Capsular Forms of Group A Streptococcal Strain 64.
Infect. Immun., 68:744-751.
150. Yamakami, K., Yoshizawa, N., Wakabayashi, K., Takeuchi, A., Tadakuma, T. and Boyle,
M.D.P. (2000) The potential role for nephritis associated plasmin receptor in acute post-
streptococcal glomerulonephritis. Methods, 21:185-197.
151. D'Costa, S., and Boyle, M.D.P. (2000) Interaction of group A streptococci expressing Glu-
plasminogen-binding M proteins with the human plasminogen system. Methods, 21:165-
177.
152. Kreikemeyer, B., Boyle, M.D.P., Buttaro, B.A., Heinemann, M. and Podbielski, A. (2000)
Group A streptococcal growth phase-associated virulence factor regulation by a novel
operon (Fas) with homologies to two-component type regulators requires a small RNA
molecule. Mol. Micro., 39:392-406.
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153. D'Costa, S., Romer, T.G. and Boyle, M.D.P. (2000) Analysis of expression of a cytosolic
enzyme on the surface of Streptococcus pyogenes. Biochem. Biophys. Res. Commun.,
278:826-832.
154. Boyle, M.D.P., Romer, T.G., Meeker, A.K. and Sledjeski, D. (2001) Use of surface
enhanced laser desorption ionization protein chip system to analyze streptococcal exotoxin
B activity secreted by Streptococcus pyogenes. J. Microb. Meth., 46:87-97.
155. Sledjeski, D.D., Coco, C., Corriher, C. and Boyle, M.D.P. (2001) Isolation of human
plasma-inducible, growth phase- and temperature-regulated gene fusions in Streptococcus
pyogenes using a Tn917-lacZ transposon. J. Micro. Meth., 46:107-117.
156. Bai, H., Buller, R.M.L., Chen, N. and Boyle, M.D.P. (2001) Viral binding proteins as
surrogates in immunoassays for immune mediators. BioTechniques, 32.160-171.
157. Saouda, M., Wu, W., Conran, P. and Boyle, M.D.P. (2001) Streptococcal pyrogenic
exotoxin B (SpeB) enhances tissue damage initiated by other Streptococcus pyogenes
products. J. Infect. Dis., 184:723-731.
158. Carr, A., Sledjeski, D.D., Podbielski, A., Boyle, M.D.P. and Kreikemeyer, B. (2001)
Similarities between complement-mediated and streptolysin S-mediated hemolysis. J. Biol.
Chem., 276:41790-41796.
159. Eberhard, T. H., Sledjeski, D.D. and Boyle, M.D.P. (2001) Mouse skin passage of a
Streptococcus pyogenes Tn917 mutant of sagA/pel restores virulence, beta-hemolysis and
sagA/pel expression without altering the position or sequence of the transposon. BMC
Microbiol. 1:33..
160 Saouda, M., Romer T. and Boyle, M.D.P. (2002) Application of immuno-mass
spectrometry to analysis of a bacterial virulence factor. BioTechniques, 32:916-92.
161. Romer T., Rescellah M. and Boyle, M.D.P. (2002) Use of surface enhanced laser
desorption ionization time-of-flight mass spectrometry to analyze post-translational
modification of bacterial virulence factors. Research Signpost. 37:1-18
162. Romer T. and Boyle, M.D.P. (2003) Application of immunoproteomics to analysis of
post-translational processing of the anti-phagocytic M protein of Streptococcus pyogenes.
Proteomics. 3:29-35.
163. Smith T., Sledjeski, D.D. and Boyle, M.D.P. (2003) Regulation of protein H
expression in M1 serotype isolates of Streptococcus pyogenes. FEMS Microbiology
Letters 219:9-15.
Michael D. Boyle
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164. Smith T., Sledjeski, D.D. and Boyle, M.D.P. (2003) Streptococcus pyogenes infection in
mouse skin leads to a time-dependent up-regulation of protein H expression. Infect.
Immun. 71:6079-6082.
165. Rezcallah MS, Boyle MD, Sledjeski DD.(2004) Mouse skin passage of Streptococcus
pyogenes results in increased streptokinase expression and activity. Microbiology
150:365-371.
166. White A.A. and Boyle M.D.P. (2004) 15:1785-1793. Raw data analysis: are students
aware of all the variables? Microbiology Education Review in press.
167. Yoshizawa, N., Wakabayaski, K., Takeuchi, A., Yamakami, K., Tadakuma, T. and Boyle,
M.D.P. (2004) Nephritis-associated Plasmin Receptor and Acute Poststreptococcal
Glomerulonephritis: Characterization of the Antigen and Associated Immune Response.
J. Am. Soc. Nephrology. 15:1785-1793.
168. Hess J., L Blazer, T. Romer, L Faber, R. M. Buller and Boyle, M.D. P(2005)
Immunoproteomics, J. Chromatog. B. 815: 65–75.
169. Blazer L, Denning E, Hemmis C, Boyle M.D. and Mulfinger L. (2005) Monitoring Peptide
Modification by SELDI-TOF Mass Spectrometry , American Laboratory, 37:15-18.
170. Coyle E, Blazer L., White A.A., Hess J.L. and Boyle M.D. (2005) Practical applications of
high-affinity, albumin-binding proteins from a group G streptococcal isolate. Applied
Microbiology and Biotechnology. 27:1-7.
171. Hess JL, Boyle MD. 2006 Fibrinogen fragment D is necessary and sufficient to anchor a
surface plasminogen-activating complex in Streptococcus pyogenes. Proteomics. 6:375-8.
172. Hess JL, Porsch EA, Shertz CA Boyle MD. (2007) Immunoglobulin-cleavage by the
streptococcal cysteine protease IdeS can be detected using protein G capture and mass
spectrometry. J Microbiological Methods , 70:284-91.
173. Boyle MD, Lottenberg R Bacterial adaptation and infection. Inflamm Allergy Drug
Targets. 2010 9(4):238-48.
174. Porsch EA, Shertz CA, Boyle MD. Novel Sample Preparation for Mass Spectral Analysis
of Complex Biological Samples. Curr Proteomics 2010 Jul;7(2):90-101.
175. Buonaccorsi VP, Boyle MD, Grove D, Praul C, Sakk E, Stuart A, Tobin T, Hosler J,
Carney SL, Engle MJ, Overton BE, Newman JD, Pizzorno M, Powell JR, Trun N.
GCAT-SEEKquence: genome consortium for active teaching of undergraduates through
Michael D. Boyle
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increased faculty access to next-generation sequencing data.CBE Life Sci Educ. 2011
10:342-5.
Chapters in Books and Other Non-Peer Reviewed Articles
1. Boyle, M.D.P. and Boros, T. (1983) Tissue damage caused by the direct and indirect
action of complement. In Reticuloendothelial Society Treatise on Immunopathology (eds.
H. Friedman, S. Reichard and M. Escobar). Plenum Press, Chapter 3, pp. 43-76.
2. Boyle, M.D.P., Faulmann, E.L., and Andres, J.M. (1988) Complement dependent
inflammatory reactions mediated by Type III bacterial Fc receptors. In Inflammatory
Bowel Disease: Current Status and Future Approaches (ed. R.P. MacDermott). Excerpta
Medica. 775:371-376.
3. Boyle, M.D.P., Lawman, M.J.P., Gee, A.P., and Young, M. (1988) Measurement
leukocyte chemotaxis In Vivo. (G. DiSabato, ed.) Methods in Enzymology, 162:101-114.
4. Boyle, M.D.P. (1990) The type I bacterial immunoglobulin binding protein:
Staphylococcal Protein A. In Bacterial Immunoglobulin Binding Proteins Volume I
(M.D.P. Boyle, ed.) Academic Press, Chapter 2 pp. 17-28.
5. Boyle, M.D.P. (1990) Complement Activation and Bacterial Immunoglobulin Binding
Proteins. In Bacterial Immunoglobulin Binding Proteins Volume I (M.D.P. Boyle, ed.)
Academic Press, Chapter 25 pp. 295-304.
6. Boyle, M.D.P. and Reis, K.J. (1990) Antigenic Relationships Among Bacterial IgG
Binding Proteins. In Bacterial Immunoglobulin Binding Proteins Volume I (M.D.P. Boyle,
ed.) Academic Press, Chapter 15 pp. 175-186.
7. Brady, J.L. and Boyle, M.D.P. (1990) IgA Fc Binding Proteins Associated with Group B
Streptococci. In Bacterial Immunoglobulin Binding Proteins Volume I (M.D.P. Boyle,
ed.) Academic Press, Chapter 18 pp. 201-225.
8. Faulmann, E.L. and Boyle, M.D.P. (1990) Type IIa and Type IIb Immunoglobulin
Binding Proteins Associated With Group A Streptococci. In Bacterial Immunoglobulin
Binding Proteins Volume I (M.D.P. Boyle, ed.) Academic Press, Chapter 6 pp. 69-83.
9. Reis, K.J. and Boyle, M.D.P. (1990) Type III Fc Immunoglobulin Binding Proteins:
Isolation and Characterization from a Group C Streptococcus. In Bacterial
Immunoglobulin Binding Proteins Volume I (M.D.P. Boyle, ed.) Academic Press, Chapter
8 pp. 101-112.
Michael D. Boyle
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10. Reis, K.J., Salpeter, J. and Boyle, M.D.P. (1990) Type IV Bacterial Immunoglobulin
Binding Proteins. In Bacterial Immunoglobulin Binding Proteins Volume I (M.D.P. Boyle,
ed.) Academic Press, Chapter 12 pp. 149-154.
11. Reis, K.J. and Boyle, M.D.P. (1990) Isolation and Characterization of a Type VI Bacterial
Immunoglobulin Binding Protein. In Bacterial Immunoglobulin Binding Proteins Volume
I (M.D.P. Boyle, ed.) Academic Press. Chapter 14 pp. 165-174.
12. Boyle, M.D.P., Faulmann, E.L., Otten, R. and Heath, D. (1990) Streptococcal
Immunoglobulin Binding Proteins. E. M. Ayoub, G. H. Cassell, W. C. Branche, T. J. Henry
(eds.) Microbial Determinants of Virulence and Host Response. A.S. M. Press Washington
D.C. pp. 19-44. Feb. 1989.
13. Boyle, M.D., Lawman, M.J.P., and Gee, A.P. (1990) Application of Fc binding proteins
for the detection of specific antibodies. In Bacterial Immunoglobulin Binding Proteins
Volume II (M.D.P. Boyle, ed.) Academic Press, pp. 181-195.
14. Boyle, M.D.P. (1990) Bacterial immunoglobulin binding proteins and complement. In
Bacterial Immunoglobulin Binding Proteins Volume II (M.D.P. Boyle, ed.) Academic
Press, pp. 369-391.
15. Boyle, M.D.P. (1990) Introduction to bacterial immunoglobulin binding proteins. In
Bacterial Immunoglobulin Binding Proteins Volume II (M.D.P. Boyle, ed.) Academic
Press, pp. 1-21.
16. Boyle, M.D.P., and Faulmann, E.L. (1990) Application of bacteria expressing
immunoglobulin binding proteins to immunoprecipitation reactions. In Bacterial
Immunoglobulin Binding Proteins Volume II (M.D.P. Boyle, ed.) Academic Press, pp.
273-289.
17. Boyle, M.D.P., Reis, K.J., and Faulmann, E.L. (1990) Isolation and functional
characterization of bacterial immunoglobulin binding proteins. In Bacterial
Immunoglobulin Binding Proteins Volume II (M.D.P. Boyle, ed.) Academic Press, pp. 71-
89.
18. Boyle, M.D.P., Reis, K.J., Otten, R.A., and Faulmann, E.L. (1990) Extraction and
monitoring of soluble immunoglobulin binding proteins. In Immunoglobulin Binding
Proteins Volume II (M.D.P. Boyle, ed.) Academic Press, pp. 49-70.
19. Brady, L., J. Musselman, C. Chun, E.M. Ayoub, and Boyle, M.D.P. (1990) Use of Fc-
binding proteins to identify cell surface and secreted antigens associated with group B
streptococci, pp. 161-179.
Michael D. Boyle
31
20. Lawman, M.J.P., Gee, A.P., Lawman, P.D., and Boyle, M.D.P. (1990) Measurement of in
vivo leucocyte chemotaxis mediated by Fc-binding proteins. In Bacterial Immunoglobulin
Binding Proteins Volume II (M.D.P. Boyle, ed.) Academic Press, pp. 405-415..
21. Otten, R.A., and Boyle, M.D.P. (1990) Bacterial immunoglobulin binding proteins -
future trends. In Bacterial Immunoglobulin Binding Proteins Volume II (M.D.P. Boyle,
ed.) Academic Press, pp. 425-452.
22. Reis, K.J., and Boyle, M.D.P. (1990) Application of enzyme labeled IgG binding proteins
in immunoassay. In Bacterial Immunoglobulin Binding Proteins Volume II (M.D.P. Boyle,
ed.) Academic Press, pp. 145-160.
23. Reis, K.J., and Boyle, M.D.P. (1990) Detection and enhancement of expression of
bacterial cell surface immunoglobulin binding proteins. In Bacterial Immunoglobulin
Binding Proteins Volume II (M.D.P. Boyle, ed.) Academic Press, pp. 23-47.
24. Reis, K.J., and Boyle, M.D.P. (1990) Production of polyclonal antibodies to
immunoglobulin binding proteins. In Bacterial Immunoglobulin Binding Proteins Volume
II (M.D.P. Boyle, ed.) Academic Press, pp. 105-124.
25. Reis, K.J., Lawman, M.J.P., and Boyle, M.D.P. (1990) Use of bacteria expressing
immunoglobulin binding proteins in coagglutination assays. In Immunoglobulin Binding
Proteins Volume II (M.D.P. Boyle, ed.) Academic Press, pp. 291-299.
26. Boyle, M.D.P., Faulmann, E. L., and Metzger, D. W. (1993) Applications of bacterial
immunoglobulin-binding proteins to the purification of immunoglobulins. In Molecular
Interactions and Bioseparation (T.T. Ngo, ed.) Plenum Press, pp. 91-112.
27. Boyle, M.D.P. (1993) Bacterial IgG-binding proteins. (M.D.P. Boyle, ed.)
ImmunoMethods, 2:1-3.
28. Boyle, M.D.P. and Metzger, D.W. (1994) Antibody-binding bacterial proteins and
immunoreagents. In Antibody Techniques. A Guide for Nonimmunologists (E.P. Lillehoj
and V.S. Malik, eds.) Academic Press, pp. 177-209.
29. Pack, T.D. and Boyle, M.D.P. (1995) The enn gene of group A streptococcal isolate A992
encodes an IgG3-binding protein. Dev. Biol. Stand. 85: 203-207.
30. Boyle, M.D.P. (1998) Bacterial IgG-binding proteins. Encyclopedia of Immunology. 2nd
edition, pp. 323-327.
31. Boyle, M.D.P. (2000) Immunoglobulin-binding proteins expressed by gram-positive
bacteria. In: Effects of Microbes on the Immune System (Madeleine W. Cunningham,
ed.), Lippincott, Williams & Wilkins, pp.195-217
Michael D. Boyle
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32. Boyle, M.D.P. and Lottenberg, R. (2000) Approaches to analysis of the role of the
plasmin(ogen) system. Editorial in Special Issue of Methods, 21:99-102.
33. Boyle, M.D.P., Raeder, R. and Podbielski, A. (2001) Ig-Binding Proteins: a Review.
EOS, 20:37-42.
34. Kreikemeyer, B., Boyle, M.D.P., Elsner, A. and Podbielski. A. (2000) Characterization of
a potential signaling peptide affecting group A streptococcal virulence factor expression.
In: Streptococcal and Streptococcal Diseases: Entering the new Millennium. (Diana R.
Martin, John R. Tagg, eds.), Proceedings of the XIV Lancefield International Symposium
on Streptococci and Streptococcal Diseases, pp. 375-379.
35. Yoshizawa, N., Yamakami, K., Fujino M., Oda T., Tamuri, K., Matsumoto K., Sugisaki T.,
and Boyle, M.D.P. (2000) The potential role for NAPLR in acute post-streptococcal
glomerulonephritis. In: Streptococcal and Streptococcal Diseases: Entering the new
Millennium. (Diana R. Martin, John R. Tagg, eds.), Proceedings of the XIV Lancefield
International Symposium on Streptococci and Streptococcal Diseases, pp. 667-671.