MicroscopesMicroscopes

Development of the Development of the microscopemicroscope

Anton van Anton van LeeuwenhoekLeeuwenhoek

Father of Father of MicrobiologyMicrobiology

Made his own lenses Made his own lenses (some as powerful as (some as powerful as the microscopes we the microscopes we use in class)use in class)

First person to see First person to see living living microorganismsmicroorganisms

Type of microscope Focuseswhat

Through/Surface

Magnifies up to

Live organisms

?

Microscopes

Compound light Compound light microscopemicroscope

Focuses lightFocuses light Shines light Shines light throughthrough

objectsobjects Magnifies up to 1000 Magnifies up to 1000

times (ours are 400x)times (ours are 400x) Can view live Can view live

organismsorganisms Uses combination of Uses combination of

lenseslenseswww.science.smith.edu/.../Labmanual/01CELLS.htm

http://www.bioweb.uncc.edu/1110Lab/notes/notes1/labpics/Elodea%20Leaf40c.jpg

Dissecting microscopeDissecting microscope

Focuses lightFocuses light See surface of objectsSee surface of objects Magnifies up to 40 Magnifies up to 40

timestimes Can view live Can view live

organismsorganisms Uses combination of Uses combination of

lenseslenses

wappingersschools.org/.../visualvocab/page4.htmlwappingersschools.org/.../visualvocab/page4.html

http://www.keweenawalgae.mtu.edu/ALGAL_IMAGES/charophyceans/Chara_d2_LApond2e_3.15x.jpg

Electron microscopeElectron microscope

Two types:Two types:

Transmission electron microscope Transmission electron microscope (TEM)(TEM)

Scanning electron microscope (SEM)Scanning electron microscope (SEM)

Transmission electron Transmission electron microscopemicroscope

Focuses electronsFocuses electrons Sees through objectsSees through objects Magnifies up to Magnifies up to

100,000 times100,000 times Can view only dead Can view only dead

or inanimate objectsor inanimate objects

www.phy.cuhk.edu.hk/.../CM120/CM120.htmlwww.phy.cuhk.edu.hk/.../CM120/CM120.html

Transmission electron Transmission electron microscopemicroscope

Mammalian mitochondrionMammalian mitochondrionbiology.unm.edu/.../Summaries/Cell.htmlbiology.unm.edu/.../Summaries/Cell.html

Scanning electron Scanning electron microscopemicroscope

Uses electronsUses electrons View surface of View surface of

objectsobjects Magnifies up to Magnifies up to

10,000 times10,000 times Views only dead or Views only dead or

inanimate objectsinanimate objects

http://gsc.nrcan.gc.ca/ebeam/sem_e.phphttp://gsc.nrcan.gc.ca/ebeam/sem_e.php

Scanning electron Scanning electron microscopemicroscope

Bee eye Bee eye www.rdg.ac.uk/cfam/facilities/sem.htmwww.rdg.ac.uk/cfam/facilities/sem.htm

Viruses buddingViruses budding

from a cellfrom a cell

Transmission Transmission electron electron

microscopemicroscope

Scanning electron Scanning electron miscroscopemiscroscope

www.www.

aecom.yu.edu/aif/gallery/sem/sem.htmaecom.yu.edu/aif/gallery/sem/sem.htm

JEOL microscopeJEOL microscope Uses finely-focused Uses finely-focused

electronselectrons surfacesurface Magnifies up to at Magnifies up to at

least 2,000,000 least 2,000,000 timestimes

Cannot view live Cannot view live organismsorganisms