Microscope Care and Use

1. Always carry the microscope by holding the arm of the microscope with one hand and supporting the base with the other hand

2. Place the microscope on a flat surface. The arm should be positioned toward you

3. Look through the eyepieces. Adjust the diaphragm so that light comes through the opening in the stage

4. Place a slide on the stage so that the specimen is in the field of view. Hold it firmly in place by using the stage clips5. Always focus first with the coarse adjustment and the low-power objective. Once the object is in focus on low power, the high-power objective can be used. Use ONLY the fine adjustment to focus the high-power lens.6. Store the microscope covered.

EYEPIECE

BODY TUBE

ARM

STAGE

COURSE ADJ.

FINE ADJUSTMENT

BASE

NOSEPIECE

OBJECTIVESTAGE CLIPSDIAPHRAGM

LIGHT SOURCE

OBJECTIVEOBJECTIVE

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Other Lab Equipment• Beakers

• Test tubes

• Flask What does all this equipment measure In the

(video) SI system?

Volume in liters.

• Biologists use a variety of tools to obtain information in an investigation.

Using tools

• Common tools include beakers, test tubes, hot plates, Petri dishes,

thermometers, balances, metric rulers, and graduated cylinders.

• Graduated cylinder:

• Thermometer:

What does this equipment measure In the SI system?

Temperature in Celsius.

What does this equipment measure In the SI system?

Volume in liters.

• Bunsen burner:

• Triple beam balance:

What does all this equipment measure In the

SI system? Mass in grams

More complex tools include:• Microscopes• Centrifuges,• Radiation detectors• Spectrophotometers• DNA analyzers • gas chromatographs

Using tools

What does the following measure?What are the SI units?

volume Liters, milliliters

What tool would one use to measure the volume of an ?

Beaker of water, measure before and after, difference is volume of eraser.

What is another tool used to measure volume?

Graduated cylinder

Flask

What is this tool called? Petri dish

What is the medium found in the Petri

Dish? Agar

What is grown on the agar?

Bacteria Colonies

What would one use to measure the mass of keys ?

Triple beam balance

What are the SI units used ?

Kilograms or grams

What would one use to measure the length of your ?

What units are used to measure length using SI?

Meter

It is the responsibility of persons conducting scientific investigations to minimize hazards to themselves and others working around them. Safety symbols warn of any dangers that may exist.

Sharp Object Safety This symbol appears when a danger of cuts or punctures caused by the use of sharp objects exists.

Clothing Protection Safety This symbol appears when substances used could stain or burn clothing.Eye Safety This symbol appears when a danger to the eyes exists. Safety goggles should be worn when this symbol appears.

Chemical Safety This symbol appears when chemicals used can cause burns or are poisonous if absorbed through the skin.

Safety Symbols