Dick JohnsonMicroVision Medical

Meibergdreef 45 • 1105 BA Amsterdam • The Netherlands

E-mail d.johnson@microvisionmedical.comU.S. Tel 1.215.498.2111 • U.S. Fax 1.201.661.2882

PRICING

www.MicroVisionMedical.com

MicroVisionMedicala view to cure

MicroVisionMedicala view to cure

CURRENT PRICE

U.S. R.O.W.

$21,000 €14.995

VAT tax or other taxes additional. Instrument includes a battery pack and a 5x probe.

R.O.W. = Rest of World

MICROSCAN VIDEO MICROSCOPEwith AVA SOFTWARE PACKAGE

TO RECEIVE MORE INFORMATION,a formal quotation or to place your order for the new MicroScan™,

please contact:

SDF Versus other Technology OPS Imaging - Sidestream Dark Field imaging has characteristics which make it superior toother modes of imaging such as OPS imaging. OPS imaging illuminates the tissues withpolarized green light and measures the reflected light from the tissue surface after filtering outthe polarized portion of the reflected light. This filters out the surface reflection of the tissuesand allows the visualization of the underlying microcirculation. Due to the reflected andemitted light passing down the same light guide (mainstream) however, OPS imaging is highlysensitive to internal scatter of light. This results inlimited visualization of the capillaries due to blurring.The technique also requires high-powered bulky lightsources, limiting its utility in difficult conditions such ascritical care or intensive care medicine.

Dark Field Imaging - Sidestream Dark Fieldimaging is different from Mainstream Dark Fieldimaging. Dark Field imaging has been described asa way of improving contrast and lowering surfacereflectance, but it typically utilizes illumination andreflectance light paths that travel down and back thesame pathway. In the past, Sidestream Dark Fieldillumination has been applied by ring illumination toimprove epi-illumination, but it has not been appliedto achieve true dark field illumination by illuminatingone segment of a substrate and observing in anothersegment the image of the microcirculation and itsflowing cells.

Ease of UseThe lightweight and portable video microscope can be configured with either 5 X or 10 Xoptical probes. The MicroScan™ probe is covered with a sterilized disposable lens thatprevents direct contact between the instrument and the patient or animal when performing avariety of microcirculation observations and analyses. The MicroScan™ is sophisticated indesign but easy to use. When attached to a lap-top computer, it is capable of capturingimages and storing them directly on the hard drive for analysis. New analysis software isavailable now for this system and provides easy analysis of the basic parameters such asdiameter, red cell velocity and functional capillary density.

MicroVisionMedicala view to cure

Camera

Magnifyinglens

Green LED

Microcirculation

Ince C: The microcirculation is the motor of sepsis. Critical Care 2005, 9(suppl 4):S13-S19.

Boerma EC, Mathura, KR, van der Voort PH, Spronk, PE, Ince, C. Quantifying beside derviced imaging of microcirculatoryabnormalities in spetic patients: a prospective validation study. Crit. Care 2005:9 (6) R601-6 Epub 2005 Sep w22

SDF andMeasurements

Sidestream Dark Fieldillumination can be used to enable thecomprehensive evaluationof the functional state ofthe microcirculation. This is achieved by an analysisof the moving cells in the images, which permitsthe quantitativemeasurement of red bloodcell flow in the capillaries.This measurement isbelieved to represent atruly sensitive measurementwhich is indicative ofcardiovascular function ordysfunction. SidestreamDark Field illuminationutilizes reflectanceavoidance in combinationwith optical magnificationto provide a superiormethod of measurement of the functional state (e.g. perfusion) of the microcirculation.Additionally, morphologicalcharacteristics of themicrocirculation, such as functional capillarydensity and micro-vesselmorphology, can bemeasured using reflectanceavoidance imaging.Homogeneous perfusion of the capillaries is aprerequisite for normal function of themicrocirculation andabnormal perfusion, ordiminished capillaryperfusion, is considered an early and sensitiveindicator of cardiovasculardisease and failure.

Principles of Sidestream DarkField Imaging(SDF)

SDF (Sidestream Dark Field)imaging utilizes a novelmethod of reflectanceavoidance in which theilluminated light andreflected light travel viaindependent pathways. In this modality, a lightguide is surrounded bygreen (530 nm) lightemitting diodes (LEDs). The light from the LEDs is absorbed by thehemoglobin of erythrocytesand results in the ability toobserve the flowing cells.The concentrically placedLEDs at the tip of the probe,protected by the disposablecap, send penetratinggreen light deep into thetissue, illuminating themicrocirculation. By notbeing in direct opticalcontact with the sensingcentral core of the probe,no direct surface reflectionsare allowed to interferewith the image of themicrocirculation. A 5 or 10times magnifying lens isused to project the imageonto a video camera,providing clear images ofthe capillaries withoutblurring and allowing forbetter computer automaticanalysis of the images.The increased depth of light penetration into thetissue using SDF allows the deeper sublingualarterioles to be clearlyobserved.

Clinical Applicability

Following its introduction in 1996, Sublingual OPS(Orthogonal Polarization Spectral) imagingrevealed the central role of the microcirculation inthe pathophysiology, outcome and treatment ofsepsis. OPS imaging allowed clinicians tovisualize for the first time significant changes in themicrocirculation under clinical conditions. Theclinical significance of these observations and themeasurement of changes in response to treatmentof the smallest microvessels, the capillaries, haveestablished the value of this technology withClinicians throughout the world. Other researchusing OPS imaging has identified additionalpotential clinical applications.

Microvision Medical Creates the Future

Microvision Medical introduces the MicroScan™, the next generation instrument whichprovides real-time intravital video images of the microcirculation. The MicroScan™ offersimproved image quality in a wide variety of clinical scenarios. Because it has lower energyrequirements, the MicroScan™ can be powered by battery and/or portable computer toilluminate low-powered high-intensity bright LEDs. These LEDs provide direct illumination onthe tissue while observations are made adjacent to the light. This novel approach is calledSidestream Dark Field (SDF) imaging. It provides more light to the specific tissue whichresults in improved image quality.

SDF Versus other Technology OPS Imaging - Sidestream Dark Field imaging has characteristics which make it superior toother modes of imaging such as OPS imaging. OPS imaging illuminates the tissues withpolarized green light and measures the reflected light from the tissue surface after filtering outthe polarized portion of the reflected light. This filters out the surface reflection of the tissuesand allows the visualization of the underlying microcirculation. Due to the reflected andemitted light passing down the same light guide (mainstream) however, OPS imaging is highlysensitive to internal scatter of light. This results inlimited visualization of the capillaries due to blurring.The technique also requires high-powered bulky lightsources, limiting its utility in difficult conditions such ascritical care or intensive care medicine.

Dark Field Imaging - Sidestream Dark Fieldimaging is different from Mainstream Dark Fieldimaging. Dark Field imaging has been described asa way of improving contrast and lowering surfacereflectance, but it typically utilizes illumination andreflectance light paths that travel down and back thesame pathway. In the past, Sidestream Dark Fieldillumination has been applied by ring illumination toimprove epi-illumination, but it has not been appliedto achieve true dark field illumination by illuminatingone segment of a substrate and observing in anothersegment the image of the microcirculation and itsflowing cells.

Ease of UseThe lightweight and portable video microscope can be configured with either 5 X or 10 Xoptical probes. The MicroScan™ probe is covered with a sterilized disposable lens thatprevents direct contact between the instrument and the patient or animal when performing avariety of microcirculation observations and analyses. The MicroScan™ is sophisticated indesign but easy to use. When attached to a lap-top computer, it is capable of capturingimages and storing them directly on the hard drive for analysis. New analysis software isavailable now for this system and provides easy analysis of the basic parameters such asdiameter, red cell velocity and functional capillary density.

MicroVisionMedicala view to cure

Camera

Magnifyinglens

Green LED

Microcirculation

Ince C: The microcirculation is the motor of sepsis. Critical Care 2005, 9(suppl 4):S13-S19.

Boerma EC, Mathura, KR, van der Voort PH, Spronk, PE, Ince, C. Quantifying beside derviced imaging of microcirculatoryabnormalities in spetic patients: a prospective validation study. Crit. Care 2005:9 (6) R601-6 Epub 2005 Sep w22

SDF andMeasurements

Sidestream Dark Fieldillumination can be used to enable thecomprehensive evaluationof the functional state ofthe microcirculation. This is achieved by an analysisof the moving cells in the images, which permitsthe quantitativemeasurement of red bloodcell flow in the capillaries.This measurement isbelieved to represent atruly sensitive measurementwhich is indicative ofcardiovascular function ordysfunction. SidestreamDark Field illuminationutilizes reflectanceavoidance in combinationwith optical magnificationto provide a superiormethod of measurement of the functional state (e.g. perfusion) of the microcirculation.Additionally, morphologicalcharacteristics of themicrocirculation, such as functional capillarydensity and micro-vesselmorphology, can bemeasured using reflectanceavoidance imaging.Homogeneous perfusion of the capillaries is aprerequisite for normal function of themicrocirculation andabnormal perfusion, ordiminished capillaryperfusion, is considered an early and sensitiveindicator of cardiovasculardisease and failure.

Principles of Sidestream DarkField Imaging(SDF)

SDF (Sidestream Dark Field)imaging utilizes a novelmethod of reflectanceavoidance in which theilluminated light andreflected light travel viaindependent pathways. In this modality, a lightguide is surrounded bygreen (530 nm) lightemitting diodes (LEDs). The light from the LEDs is absorbed by thehemoglobin of erythrocytesand results in the ability toobserve the flowing cells.The concentrically placedLEDs at the tip of the probe,protected by the disposablecap, send penetratinggreen light deep into thetissue, illuminating themicrocirculation. By notbeing in direct opticalcontact with the sensingcentral core of the probe,no direct surface reflectionsare allowed to interferewith the image of themicrocirculation. A 5 or 10times magnifying lens isused to project the imageonto a video camera,providing clear images ofthe capillaries withoutblurring and allowing forbetter computer automaticanalysis of the images.The increased depth of light penetration into thetissue using SDF allows the deeper sublingualarterioles to be clearlyobserved.

Clinical Applicability

Following its introduction in 1996, Sublingual OPS(Orthogonal Polarization Spectral) imagingrevealed the central role of the microcirculation inthe pathophysiology, outcome and treatment ofsepsis. OPS imaging allowed clinicians tovisualize for the first time significant changes in themicrocirculation under clinical conditions. Theclinical significance of these observations and themeasurement of changes in response to treatmentof the smallest microvessels, the capillaries, haveestablished the value of this technology withClinicians throughout the world. Other researchusing OPS imaging has identified additionalpotential clinical applications.

Microvision Medical Creates the Future

Microvision Medical introduces the MicroScan™, the next generation instrument whichprovides real-time intravital video images of the microcirculation. The MicroScan™ offersimproved image quality in a wide variety of clinical scenarios. Because it has lower energyrequirements, the MicroScan™ can be powered by battery and/or portable computer toilluminate low-powered high-intensity bright LEDs. These LEDs provide direct illumination onthe tissue while observations are made adjacent to the light. This novel approach is calledSidestream Dark Field (SDF) imaging. It provides more light to the specific tissue whichresults in improved image quality.

Dick JohnsonMicroVision Medical

Meibergdreef 45 • 1105 BA Amsterdam • The Netherlands

E-mail d.johnson@microvisionmedical.comU.S. Tel 1.215.498.2111 • U.S. Fax 1.201.661.2882

PRICING

www.MicroVisionMedical.com

MicroVisionMedicala view to cure

MicroVisionMedicala view to cure

CURRENT PRICE

U.S. R.O.W.

$21,000 €14.995

VAT tax or other taxes additional. Instrument includes a battery pack and a 5x probe.

R.O.W. = Rest of World

MICROSCAN VIDEO MICROSCOPEwith AVA SOFTWARE PACKAGE

TO RECEIVE MORE INFORMATION,a formal quotation or to place your order for the new MicroScan™,

please contact: